ABM Reagents

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

DataSheet

MSDS

PCR Product Guide

miRNA All-In-One cDNA Synthesis Kit

G898 miRNA All-In-One cDNA Synthesis Kit by Applied Biological Materials provides a 20-reaction, single-tube poly(A) tailing + reverse transcription workflow for miRNA first-strand cDNA synthesis using Poly(A) Polymerase, OneScript&reg; Plus RT, and a modified Oligo d(T) Adapter for universal miRNA coverage. Compatible with BlasTaq&trade; qPCR (G891). Store at &minus;20&deg;C; stable 2 years.

Unit Quantity

Storage Condition

Shipping Condition

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Mitochondria Labeling Lentivirus (MTS)(GFP)

LVP721 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing GFP under a mitochondria-targeting sequence (MTS) for stable, specific green fluorescence labeling of mitochondria in transduced mammalian cells. GFP companion to LVP722 (MTS-RFP). For live-cell mitochondrial dynamics imaging, mitophagy assays (GFP quenches in lysosomes), and two-color co-localization with RFP markers (LVP731). Store at &minus;80°C.

Vector

Titer	

Mitochondria Labeling Lentivirus (MTS)(RFP)

LVP722 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing RFP under a mitochondria-targeting sequence (MTS) for stable, specific red fluorescence labeling of mitochondria in transduced mammalian cells. For live-cell mitochondrial dynamics imaging, mitophagy assays, and co-localization studies. Store at &minus;80°C; single-use aliquots.

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
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Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

mKate2-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)

AX0010032 mKate2-P2A-LOCa3 by Applied Biological Materials is a lentiviral construct that co-expresses LOCa3 and mKate2 far-red fluorescent protein via a P2A self-cleaving peptide for stoichiometric bicistronic co-expression and constitutive fluorescent cell tracking. Store at &minus;80&deg;C.

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
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Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
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<h2 data-path-to-node="5">What you will find:</h2>
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<ul data-path-to-node="6">
<li style="font-family: arial, helvetica, sans-serif;">
Tissue-specific primary cell culture medium kits
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Specialized growth media for endothelial and epithelial cells
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Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
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<li style="font-family: arial, helvetica, sans-serif;">
Differentiation media for progenitor and stem cells&nbsp;
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<li style="font-family: arial, helvetica, sans-serif;">
Serum-free bioprocessing and cryopreservation reagents
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<li style="font-family: arial, helvetica, sans-serif;">
Targeted fibroblast, smooth muscle, and myocyte growth kits
</li>
</ul>
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<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
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Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
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Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Multi-Lineage Progenitor Cell Differentiation Media Kit

Multi-Lineage Progenitor Cell Differentiation Media Kit (TM114) by Applied Biological Materials is an antibiotic-free PriGrow X Series kit (TM114-C basal medium + TM114-C-S supplement mix) for in vitro differentiation of multi-lineage progenitor cells. Complete medium expires 30 days post-supplementation. May become more opaque after mixing — expected. Store basal at 4–8°C; supplement at –20°C.

Sterility

Appearance

Disclaimer

Myc EYFP Reporter Adenovirus

000774A by Applied Biological Materials is the Stemporter™ Myc EYFP Reporter Adenovirus (1.0 ml in DMEM/2.5% glycerol) expressing EYFP under a Myc promoter for non-integrating, transient identification of Myc-active iPSC clones by yellow fluorescence. EYFP excitation ~514 nm, emission ~527 nm. Spectrally compatible with Oct4-ECFP reporter (000776A) for two-color Stemporter experiments.

<h2 data-section-id="1fu0kiw" data-start="0" data-end="45">What are additional cell culture reagents?</h2>
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Additional cell culture reagents are specialized products used to support cell culture procedures that extend beyond routine media preparation, supplementation, and contamination control. These reagents perform specific functions such as cryopreservation, stable cell line selection, transfection, cell attachment enhancement, suspension culture protection, and metabolic supplementation.
While basal media, sera, buffers, and standard supplements provide the foundation for cell growth, additional cell culture reagents help researchers manipulate, preserve, modify, and optimize cells for specialized applications in research, biotechnology, and biomanufacturing.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
High-Purity Animal Sera for enhanced cell proliferation and maintenance, e.g., USDA Fetal Bovine Serum (FBS)&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Specialized Growth Supplements for primary cell cultivation, e.g., Macrophage Culture Supernatants and ECGS
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Cell Viability and Counting Stains for accurate analysis of cellular density and health, e.g., Standardized Trypan Blue solutions&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Isotonic Buffer Solutions for reagent dilution and gentle cell washing, e.g., High-quality PBS and DPBS and&nbsp; formulations
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Antimicrobial and Hormonal Additives for optimization of cultures, e.g., Hydrocortisone and targeted Mycoplasma elimination cocktails
</li>
</ul>
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<h2 data-section-id="1e70xzx" data-start="889" data-end="938">How to choose additional cell culture reagents</h2>
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Cryopreservation reagents
Cryopreservation reagents protect cells during freezing and long-term storage. Dimethyl sulfoxide (DMSO) remains the most widely used cryoprotectant and is commonly included at 5&ndash;10% concentration in freezing media to reduce ice crystal formation that can damage cell membranes.
Ready-to-use freezing media are available in both DMSO-containing and DMSO-free formulations, allowing researchers to select the most appropriate option for sensitive cell types and specialized applications.
Selection antibiotics
Selection antibiotics are used to identify and maintain cells that have successfully incorporated a genetic construct carrying a corresponding resistance gene.
The optimal working concentration must be determined experimentally using a kill curve to identify the lowest concentration that completely eliminates non-resistant cells.
Transfection reagents
Transfection reagents facilitate the delivery of DNA, RNA, or gene-editing components into cultured cells.
Cationic lipid reagents are among the most commonly used transfection tools because they form complexes with nucleic acids that can enter cells through endocytosis. Polybrene is frequently used during viral transduction to improve vector binding and increase transduction efficiency.
Attachment factors
Certain primary cells, neuronal cultures, stem cells, and other sensitive cell types require enhanced surface adhesion. Attachment factors are applied as coatings to culture vessels before cell seeding.
Common attachment factors include:
<ul data-start="2797" data-end="2857">
<li data-section-id="vje5ia" data-start="2797" data-end="2812">Poly-D-lysine</li>
<li data-section-id="1gc9n1l" data-start="2813" data-end="2823">Collagen</li>
<li data-section-id="qxi803" data-start="2824" data-end="2837">Fibronectin</li>
<li data-section-id="x7geko" data-start="2838" data-end="2847">Laminin</li>
<li data-section-id="ixblas" data-start="2848" data-end="2857">Gelatin</li>
</ul>
These coatings improve cell attachment, spreading, survival, and differentiation.
Cell-protective agents
Suspension cultures and bioreactor systems expose cells to mechanical stress from agitation and aeration. Cell-protective surfactants such as Pluronic F-68 help reduce shear damage and improve cell viability during large-scale culture processes.
Glucose supplementation
Some cell culture protocols require glucose concentrations higher than those supplied by the basal medium. Concentrated glucose solutions can be added to support high-density cultures, serum-free systems, or specialized metabolic studies.
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<h2 data-section-id="1fljvas" data-start="4355" data-end="4380">Specifications context</h2>
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Selection antibiotic optimization remains a critical step in stable cell line development, with kill-curve experiments routinely performed before large-scale selection. Attachment factors continue to play an important role in primary cell, neuronal, and stem cell culture, while Pluronic F-68 remains widely used in suspension and bioreactor-based production systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to request a quote for downstream-ready additional cell culture reagents.

Additional Cell Culture Reagents

G398-MSDS

Flyer

Mycoplasma Elimination Cocktail

MycoAway™ 1000X Mycoplasma Cocktail (G398) by Applied Biological Materials is a 1.0 ml 1000X concentrate that eliminates 70+ Mycoplasma species from adherent and suspension cell cultures in 1–2 weeks (2–3 passages), non-toxic at 1X working concentration. Pair with G238 or G239 for complete detect-and-eliminate workflow. Store at –20°C.

<h2 dir="ltr">What Is a Mycoplasma PCR Detection Kit?</h2>
 A Mycoplasma PCR detection kit provides primers, master mix, ultrapure water, and a positive control for rapid PCR-based screening of cell culture samples for Mycoplasma contamination. Because Mycoplasma species lack a cell wall and do not produce visible turbidity or media discoloration, contamination frequently goes undetected without periodic molecular testing, making these kits a standard component of cell culture quality assurance programmes. Run a Mycoplasma PCR detection kit periodically on actively maintained cell lines and before banking, sharing, or publishing results from a cell line as routine quality assurance against a contaminant that produces no visible signs. 
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Detection &amp; Elimination Kits: All-encompassing systems such as Mycoplasma Pro crafted to accurately identify contamination while also enabling complete eradication of infected cultures.
</li>
<li dir="ltr" aria-level="1">
PCR Detection Kits: Standardized, validated reagents that provide a quick and economical solution for regular laboratory testing and quality assurance.
</li>
<li dir="ltr" aria-level="1">
Mycoplasma gDNA Kits: Tailored genomic DNA extraction solutions specifically designed for isolating low-abundance mycoplasma templates from intricate biological specimens.
</li>
</ul>
<h2 dir="ltr"> How to Choose a Mycoplasma PCR Detection Kit</h2>
 Species/Strain Coverage Kits vary in the breadth of Mycoplasma genera and species detected -- some formats detect over 200 strains, while enhanced ("Pro"-type) formats may extend coverage to 300+ strains, targeting conserved regions shared across common contaminant species such as M. arginini, M. hyorhinis, M. orale, and others. Sample Preparation Requirements Many Mycoplasma PCR detection kits allow direct addition of cell culture media to the PCR reaction without a separate DNA extraction step, simplifying the workflow and reducing time-to-result -- confirm whether your chosen kit requires extraction or supports direct sample addition. Internal Control Availability Enhanced kit formats may include an internal amplification control, providing additional confidence that a negative result reflects true absence of contamination rather than a failed or inhibited PCR reaction -- particularly valuable for samples with potential PCR inhibitors. Detection Sensitivity Standard kits can detect contamination at typical levels found in actively growing contaminated cultures; ultra-sensitive qPCR-based Mycoplasma detection formats (a related but distinct product type) can detect as few as 10 genome copies directly from media for earlier-stage contamination detection. Result Interpretation A positive result is typically indicated by a PCR product in a defined size range (commonly in the 260-550 bp range depending on the specific primer design), distinguishable from the positive control band; a clean no-template control validates assay specificity for each run. 
<h2 dir="ltr"> Specifications Context</h2>
 Mycoplasma PCR detection kits use primers highly specific to conserved rDNA regions across Mycoplasma genomes, enabling a single primer mix to detect dozens to hundreds of species and strains -- including commonly encountered laboratory contaminants - in one reaction. The streamlined workflow (combine sample with master mix and primer mix, run PCR, analyse by gel electrophoresis) typically delivers results within 1-2 hours, in contrast to culture-based Mycoplasma testing methods that can take days to weeks. A positive result appears as a distinct PCR product within the kit's expected size range, while the included positive control confirms the reaction performed correctly even when test samples are negative. Routine screening intervals vary by institutional policy, but testing before banking a new cell line, after receiving cells from an external source, and periodically during long-term culture are common checkpoints. Mycoplasma PCR detection kits are typically stored at &minus;20 &deg;C; positive controls are included with the kit. 
Safeguard the integrity of your culture&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">reach out to the MBP team</a> now for a quote on our expert Mycoplasma detection services.

Mycoplasma PCR Detection Kit

Mycoplasma PCR Detection & Elimination Kit

Mycoplasma PCR Detection &amp; Elimination Kit (G238-G398) by Applied Biological Materials combines the Mycoplasma PCR Detection Kit (G238, 200+ strains, &lt;2 hr, no DNA isolation) with MycoAway™ 1000X Mycoplasma Cocktail (G398, 70+ species eliminated in 2–3 weeks). ISO 13485:2016 MDSAP certified. Shipped on ice pack.

Mycoplasma Pro PCR Detection Kit Competitor Comparison

Mycoplasma Pro PCR Detection & Elimination Kit

Mycoplasma Pro PCR Detection &amp; Elimination Kit (G239-G398) by Applied Biological Materials combines the upgraded G239 Pro kit (300+ strains, internal PCR control, A. laidlawii coverage, &lt;2 hr) with MycoAway™ G398 (70+ species eliminated in 2–3 weeks). ISO 13485:2016 MDSAP certified. Shipped on ice pack.

List of Detectable Species/Strains (Cat. G239)

Mycoplasma Pro PCR Detection Kit

Mycoplasma Pro PCR Detection Kit (G239) by Applied Biological Materials is a 100-reaction PCR kit detecting 300+ Mycoplasma and Acholeplasma strains (including A. laidlawii) in under 2 hours with no DNA isolation. Includes internal PCR control, BlasTaq™ MasterMix, Pro Primer Mix, and positive control. ISO 13485:2016 MDSAP certified. Store at –20°C; 2-year stability.

Myocyte Medium Kit

Myocyte Medium Kit (TM072) by Applied Biological Materials is a low-serum, antibiotic-free kit (basal medium TM072 + supplement mix TM072-S) formulated to support robust myocyte growth while maintaining characteristic morphology and markers in vitro. Complete medium expires 30 days post-supplementation. Store basal at 4–8°C; supplement at –20°C.

Ng2 promoter-CRE-IRES-dsRED Adenovirus

000844A Ng2 Promoter-CRE-IRES-dsRED Adenovirus by Applied Biological Materials expresses CRE recombinase under the NG2 cell-type-specific promoter with IRES-linked dsRED reporter for transient, non-integrating CRE delivery selectively in NG2+ cells (pericytes, OPCs). 1.0 ml in DMEM/2.5% glycerol; store at &minus;80&deg;C.