Name: Ni-IDA Agarose Resins
SKU: G250
Availability: InStock

<h2>What are Protein Crosslinking, Labeling, and Modification Reagents?</h2>
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These reagents covalently bond proteins together or attach functional labels to proteins for downstream analysis. They are broadly divided into homobifunctional and heterobifunctional crosslinkers based on the type of reactive groups they contain.
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<h2 dir="ltr">What you will find:</h2>
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Ni-IDA Agarose Resins: High-capacity purification systems (G250) utilizing immobilized Ni2+ cations and iminodiacetic acid groups for the rapid, one-step isolation of His-tagged proteins under both native and denaturing conditions.
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Versatile Purification Formats: Ready-to-use resins suitable for batch or gravity-flow chromatography, offering an economical and reusable solution for antibody purification and the study of protein-DNA interactions at any scale.
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<h2>How to Choose Crosslinking and Labeling Reagents</h2>
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Decide Between Homobifunctional and Heterobifunctional Chemistry
Homobifunctional crosslinkers contain identical reactive groups at both ends and are used to stabilize or capture existing protein interactions. Heterobifunctional crosslinkers contain two different reactive groups, allowing controlled stepwise conjugation between specific molecules.
Choose Water-Soluble Crosslinkers for Aqueous or Cellular Work
BS3 is the water-soluble form of DSS and is preferred for reactions in aqueous buffers or intact cell systems while maintaining the same chemistry and spacer length.
Match Spacer Arm Length to Molecular Distance
The spacer arm determines the maximum distance between two reactive groups that can be linked. Incorrect spacing can reduce efficiency or increase non-specific crosslinking.
Use Heterobifunctional Chemistry for Defined Conjugates
Sulfo-SMCC enables sequential coupling of amine-containing molecules with sulfhydryl-containing partners, commonly used for antibody-enzyme or antibody-carrier conjugation workflows.
Consider Cleavable Crosslinkers for Reversible Studies
Cleavable crosslinkers such as DTSSP contain disulfide bonds that can be reduced later, enabling separation of linked proteins for downstream analysis such as mass spectrometry.
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<h2>Specifications Context</h2>
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DSS has an 11.4 &Aring; spacer arm that defines the maximum distance between linked amine groups, while BS3 provides the same chemistry with improved water solubility for aqueous applications. Cleavable crosslinkers are increasingly used in mass spectrometry workflows for mapping protein-protein interactions.
Streamline your protein modification workflows&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Ni-IDA resin solutions.

Crosslinking, Labeling and Protein Modification

Ni-IDA Agarose Resins

Ni-IDA Agarose Resins (G250, G251, G253) by Applied Biological Materials are nickel-charged IDA agarose resins for IMAC purification of 6×His- and 10×His-tagged recombinant proteins under native or denaturing conditions. Available in multiple pack sizes.