ABM Reagents

<h2 dir="ltr">What Are Luciferase Reporter Lentiviral Vectors?</h2>
 Luciferase reporters have been foundational to gene expression research since their first use as reporter genes in 1985. The bioluminescent signal produced when luciferase oxidises its substrate provides a sensitive, quantitative, non-radioactive readout measurable over a wide dynamic range with standard plate reader luminometers. Lentiviral delivery produces stable, integrated reporter cell lines that maintain consistent signal over weeks to months. Accurate reporter assays depend on appropriate detection systems. Select a luciferase construct that fits your research needs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Luciferase Reporters: Reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background controls with <a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">luciferase reporter systems</a>.</li>
<li>Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li>Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li>Promoter &amp; UTR Reporter Systems: Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li>Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose a Luciferase Reporter</h2>
 Reporter Role Firefly luciferase is typically driven by the promoter or response element under investigation as the experimental reporter; Renilla luciferase &mdash; driven by a constitutive promoter (SV40 or CMV) &mdash; provides within-sample normalisation correcting for cell number, transfection efficiency, and lysis variability. Substrate and Emission Firefly luciferase uses D-luciferin and emits at ~550&ndash;570 nm (green/yellow); Renilla uses coelenterazine and emits at ~480 nm (blue-green) &mdash; both can be measured sequentially from the same lysate in a dual-luciferase assay. Stable vs. Transient Use Lentiviral delivery produces stable reporter cell lines in 1&ndash;2 weeks via antibiotic selection &mdash; essential for high-throughput compound screening, long-term pathway activity monitoring, and in vivo bioluminescence imaging. Pathway-Specific Reporters Promoter or transcriptional response element (TRE) inserts &mdash; such as Wnt/TCF-LEF, NF-&kappa;B, NFAT, or VEGF promoter &mdash; provide a quantitative readout of pathway activity when the cognate transcription factor is activated. In Vivo Imaging Needs CMV-GFP-T2A-Luciferase dual-reporter lentivectors co-express GFP and luciferase for combined fluorescence and bioluminescence tracking of implanted cells in live animals. 
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 Cells are lysed and mixed with the appropriate substrate in a plate reader or tube luminometer; light emission is measured in relative light units (RLU), proportional to luciferase activity and reflecting promoter activity or transgene expression level. A promoter-less dual luciferase vector establishes the minimum signal level for the assay system, identifying the true zero-activity baseline. Stable lentiviral luciferase reporter cell lines are essential tools for tracking xenograft tumour growth, metastasis, and therapy response in preclinical mouse models. EF1&alpha;-driven constructs maintain high expression for 30+ days without selection, while CMV-driven constructs may undergo progressive silencing in some cell types. Product availability reflects MBP's catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get optimized luciferase reporters for advanced cloning and CRISPR-related workflows.

Luciferase Reporter

AAV Packaging Protocol

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Luciferase-mCherry Lentivirus (CMV)

LVP010138 by Applied Biological Materials is a pre-packaged Luciferase-mCherry dual-reporter lentiviral virus. mCherry (CMV, constitutive) + Photinus pyralis Luc2 (3×HRE-SV40, hypoxia-inducible, D-luciferin). Kanamycin + Puromycin selection. 108 IU/mL, 3 × 250 µL. Plasmid: LV010138. Store at −80°C; shipped on dry ice.

Unit Quantity

Accession Number

Species

Vector Map

Titer

Shipping Condition

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Lysosome Labeling Lentivirus (LAMP1)(GFP)

LVP718 Lysosome Labeling Lentivirus (LAMP1)(GFP) by Applied Biological Materials delivers LAMP1-GFP for stable green fluorescent lysosomal labeling in transduced mammalian cells. Green-channel counterpart to LVP719 (LAMP1-RFP); spectrally compatible with RFP-based organelle markers for dual-color imaging. Store at &minus;80&deg;C.

Vector

Titer	

Storage Condition

Lysosome Labeling Lentivirus (LAMP1)(RFP)

LVP719 Lysosome Labeling Lentivirus (LAMP1)(RFP) by Applied Biological Materials delivers a LAMP1-RFP fusion for stable red fluorescent labeling of the lysosomal compartment in transduced mammalian cells. Spectrally compatible with GFP-based markers for dual-channel imaging; store at &minus;80&deg;C.

<h2 data-section-id="185nm5c" data-start="0" data-end="38">What is classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture for decades, including DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's F-10, Ham's F-12, IMDM (Iscove's Modified Dulbecco's Medium), and DMEM/F-12. These media provide the amino acids, vitamins, glucose, salts, buffering agents, and other nutrients required to support cell survival and growth in vitro. They form the basis of many modern specialized, serum-free, and advanced media formulations used in academic research, biotechnology, pharmaceutical development, and biomanufacturing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
&nbsp;Availability of both low and high carbohydrate levels of balanced basal formulations for the distinct metabolic requirements of diverse mammalian cell lines.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Maintenance of precise physiological pH during long-term incubation cycles using optimized buffering configurations with integrated chemical stabilizers&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Nutrient-dense cell media supplemented with vitamins and essential amino acids to support&nbsp; hybridomas and rapidly proliferating continuous cultures
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Optimized serum reduction along with enhanced cell attachment using&nbsp;Multi-purpose 1:1 formulation hybrids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Salt solutions supplemented with essential minerals to facilitate enzyme preparation, cell washing, and reliable short-term osmotic maintenance.
</li>
</ul>
&nbsp;
<h2 data-section-id="1blio5n" data-start="931" data-end="964">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acids and vitamins found in MEM, along with higher glucose concentrations, making it suitable for cell types with elevated nutrient demands. It is widely used for primary cells, stem cells, and many transformed mammalian cell lines. MEM, based on the original Eagle's formulation, remains a common choice for standard monolayer culture and is available with different salt formulations to support various applications.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640 was originally developed for culturing human leukemic cells and remains the preferred medium for lymphocytes, hybridomas, and other hematopoietic cell types. Its formulation includes glutathione and elevated levels of biotin, vitamin B12, and PABA. Although designed for suspension cultures, RPMI 1640 also supports a wide range of adherent cell lines when appropriately supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were developed for CHO cells and other specialized cell types. Their formulations include additional nutrients such as zinc, putrescine, hypoxanthine, and thymidine, making them useful for hepatocyte culture, toxicity testing, viral fusion studies, and other specialized applications. These media can be used with or without serum depending on the experimental requirements.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 combines the nutrient-rich composition of DMEM with the specialized components found in Ham's F-12. The resulting formulation supports demanding cell types including MDCK cells, glial cells, fibroblasts, and endothelial cells. It is also widely used as the foundation for many serum-free and advanced culture systems.
IMDM for high-density rapid proliferation
IMDM is an enriched derivative of DMEM that contains additional amino acids, vitamins, HEPES buffer, sodium pyruvate, and potassium nitrate. Its enhanced nutrient profile makes it particularly suitable for rapidly proliferating and high-density cultures such as Jurkat cells, COS-7 cells, and macrophages.
Liquid vs powder format
Liquid classic media are supplied ready for use after the addition of serum and required supplements. Powdered media offer lower shipping costs and greater flexibility for high-volume laboratories but require reconstitution, sodium bicarbonate addition, and sterile filtration before use.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4382" data-end="4407">Specifications context</h2>
&nbsp;
Classic media are manufactured according to their original published formulations, with only stability-related modifications where necessary. Most liquid formulations contain sodium bicarbonate as the primary buffering system and require incubation in a 5&ndash;10% CO₂ atmosphere to maintain physiological pH. Powdered formulations require reconstitution, sodium bicarbonate supplementation, and sterile filtration before use.
As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the most widely used classic cell culture media across academic, biotechnology, pharmaceutical, and biomanufacturing laboratories. IMDM continues to gain popularity for high-density culture and bioprocessing applications.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents such as GlutaMAX, NEAA, sodium pyruvate, and HEPES, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized classic media for your lab.

Classic Media

M199 Medium

M199 Medium (TM502) by Applied Biological Materials is a 500 ml sterile basal medium containing L-glutamine, sodium bicarbonate (2.2 g/L), and phenol red (no HEPES) for broad-species primary cell culture, virology, and vaccine production. Supplement as required; requires 5–10% CO₂. Store at 2–8°C, protected from light.

Sterility

Appearance

Disclaimer

Caution

<h2 data-section-id="1fu0kiw" data-start="0" data-end="45">What are additional cell culture reagents?</h2>
&nbsp;
Additional cell culture reagents are specialized products used to support cell culture procedures that extend beyond routine media preparation, supplementation, and contamination control. These reagents perform specific functions such as cryopreservation, stable cell line selection, transfection, cell attachment enhancement, suspension culture protection, and metabolic supplementation.
While basal media, sera, buffers, and standard supplements provide the foundation for cell growth, additional cell culture reagents help researchers manipulate, preserve, modify, and optimize cells for specialized applications in research, biotechnology, and biomanufacturing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
High-Purity Animal Sera for enhanced cell proliferation and maintenance, e.g., USDA Fetal Bovine Serum (FBS)&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Specialized Growth Supplements for primary cell cultivation, e.g., Macrophage Culture Supernatants and ECGS
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Cell Viability and Counting Stains for accurate analysis of cellular density and health, e.g., Standardized Trypan Blue solutions&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Isotonic Buffer Solutions for reagent dilution and gentle cell washing, e.g., High-quality PBS and DPBS and&nbsp; formulations
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Antimicrobial and Hormonal Additives for optimization of cultures, e.g., Hydrocortisone and targeted Mycoplasma elimination cocktails
</li>
</ul>
&nbsp;
<h2 data-section-id="1e70xzx" data-start="889" data-end="938">How to choose additional cell culture reagents</h2>
&nbsp;
Cryopreservation reagents
Cryopreservation reagents protect cells during freezing and long-term storage. Dimethyl sulfoxide (DMSO) remains the most widely used cryoprotectant and is commonly included at 5&ndash;10% concentration in freezing media to reduce ice crystal formation that can damage cell membranes.
Ready-to-use freezing media are available in both DMSO-containing and DMSO-free formulations, allowing researchers to select the most appropriate option for sensitive cell types and specialized applications.
Selection antibiotics
Selection antibiotics are used to identify and maintain cells that have successfully incorporated a genetic construct carrying a corresponding resistance gene.
The optimal working concentration must be determined experimentally using a kill curve to identify the lowest concentration that completely eliminates non-resistant cells.
Transfection reagents
Transfection reagents facilitate the delivery of DNA, RNA, or gene-editing components into cultured cells.
Cationic lipid reagents are among the most commonly used transfection tools because they form complexes with nucleic acids that can enter cells through endocytosis. Polybrene is frequently used during viral transduction to improve vector binding and increase transduction efficiency.
Attachment factors
Certain primary cells, neuronal cultures, stem cells, and other sensitive cell types require enhanced surface adhesion. Attachment factors are applied as coatings to culture vessels before cell seeding.
Common attachment factors include:
<ul data-start="2797" data-end="2857">
<li data-section-id="vje5ia" data-start="2797" data-end="2812">Poly-D-lysine</li>
<li data-section-id="1gc9n1l" data-start="2813" data-end="2823">Collagen</li>
<li data-section-id="qxi803" data-start="2824" data-end="2837">Fibronectin</li>
<li data-section-id="x7geko" data-start="2838" data-end="2847">Laminin</li>
<li data-section-id="ixblas" data-start="2848" data-end="2857">Gelatin</li>
</ul>
These coatings improve cell attachment, spreading, survival, and differentiation.
Cell-protective agents
Suspension cultures and bioreactor systems expose cells to mechanical stress from agitation and aeration. Cell-protective surfactants such as Pluronic F-68 help reduce shear damage and improve cell viability during large-scale culture processes.
Glucose supplementation
Some cell culture protocols require glucose concentrations higher than those supplied by the basal medium. Concentrated glucose solutions can be added to support high-density cultures, serum-free systems, or specialized metabolic studies.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4355" data-end="4380">Specifications context</h2>
&nbsp;
Selection antibiotic optimization remains a critical step in stable cell line development, with kill-curve experiments routinely performed before large-scale selection. Attachment factors continue to play an important role in primary cell, neuronal, and stem cell culture, while Pluronic F-68 remains widely used in suspension and bioreactor-based production systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to request a quote for downstream-ready additional cell culture reagents.

Additional Cell Culture Reagents

Macrophage Culture Supplement/L-cell Supernatant

Macrophage Culture Supplement / L-cell Supernatant (TM142) by Applied Biological Materials is a 40 ml sterile M-CSF-rich conditioned medium supplement for macrophage growth media, supporting BMDM differentiation and primary macrophage culture for up to 7 days. Lot-tested for sterility, pH, osmolality, and endotoxin. Store at –20°C.

Shipping Conditions

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
&nbsp;
Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
&nbsp;
<h2 data-path-to-node="5">What you will find:</h2>
&nbsp;
<ul data-path-to-node="6">
<li style="font-family: arial, helvetica, sans-serif;">
Tissue-specific primary cell culture medium kits
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Specialized growth media for endothelial and epithelial cells
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Differentiation media for progenitor and stem cells&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Serum-free bioprocessing and cryopreservation reagents
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Targeted fibroblast, smooth muscle, and myocyte growth kits
</li>
</ul>
&nbsp;
<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
&nbsp;
Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
&nbsp;
Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Flyer

Mammary Epithelial Medium Kit

Mammary Epithelial Medium Kit (TM043) by Applied Biological Materials — 500 mL Basal Medium with antibiotic-free Supplement Mix. Low-serum, growth factor-supplemented formulation for mammary epithelial cell culture. QC-tested for sterility, pH, endotoxin, and cell performance. 30-day shelf life post-preparation.

McCoy’s 5A  Medium

McCoy's 5A Medium (TM506) by Applied Biological Materials is a 500 ml sterile basal medium (osmolarity 270–330 mOsm/kg) containing L-glutamine, sodium bicarbonate (2.2 g/L), and phenol red — without HEPES or sodium pyruvate — for primary cultures from adrenal, bone marrow, lung, kidney, and other tissues. Supplement with 10% FBS; requires 5–10% CO₂. Store at 2–8°C, protected from light.

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

One-Step RT-PCR Kits

DataSheet

MSDS

PCR Product Guide

MegaFi One-Step RT-PCR Kit

G597 by Applied Biological Materials is the MegaFi&trade; Pro One-Step RT-PCR Kit (100 rxn) — single-tube RT + PCR with OneScript&reg; Hot RT (thermostable, 50–80°C cDNA synthesis), MegaFi&trade; Pro Fidelity Polymerase (&gt;2000X lower error than Taq), and RNaseOFF Inhibitor. 2X Buffer includes gel loading dye. ISO 13485:2016 MDSAP Certified. Store at &minus;20°C.

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
</ul>
<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

High-Fidelity Master Mix

MegaFi™ Pro Fidelity 2X Mastermix

MegaFi Pro Fidelity 2X PCR MasterMix

G887 MegaFi&trade; Pro Fidelity 2X PCR MasterMix by Applied Biological Materials is an ultra-high-fidelity 2X PCR master mix with &gt;2,000&times; lower error rate than Taq, Hot Start monoclonal antibody, blunt-end products, and integrated gel loading dye. 800 rxn (10.0 ml); ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

Concentration

<h2>What Are High-Fidelity DNA Polymerases?</h2>
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The development of high-fidelity thermostable DNA polymerases transformed PCR from a tool for detection and amplification into a precise DNA engineering platform. By combining thermostability with 3' to 5' proofreading exonuclease activity, modern high-fidelity enzymes detect and remove misincorporated bases immediately after insertion during DNA synthesis. Polymerase fidelity can significantly affect downstream results, so choosing an enzyme that meets your accuracy and performance requirements is essential.
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<h2>What you will find:</h2>
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<ul>
<li>High-fidelity DNA amplification with proofreading activity using Azura TruFi&trade; II DNA Polymerase.</li>
<li>Proofreading polymerase formulations with matched reaction buffers, including ExtremeTaq&trade; DNA Polymerase with 5x Reaction Buffer.</li>
<li>Accurate PCR amplification with Accuris High Fidelity DNA Polymerase for cloning, sequencing, and mutagenesis workflows.</li>
<li>High-fidelity enzyme systems featuring proofreading activity, including MegaFi Pro Fidelity DNA Polymerase.</li>
</ul>
&nbsp;
<h2>How to Choose a High-Fidelity DNA Polymerase</h2>
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Enzyme Family Selection
High-fidelity polymerases differ in processivity, extension speed, fidelity, and template compatibility. Selection should consider amplicon length, GC content, template complexity, and downstream application requirements.
Format: Enzyme vs. Master Mix
Individual high-fidelity enzymes with optimized HF buffers suit custom reaction optimization, particularly for difficult templates requiring buffer additive titration. In contrast, 2x master mixes streamline routine high-fidelity amplification for cloning and mutagenesis workflows.
Error Rate Requirements
For applications where amplicons will be cloned and sequence-verified downstream, the approximately 50- to 100-fold lower error rate of high-fidelity enzymes compared with standard Taq polymerase substantially reduces the frequency of sequence-confirmed clones carrying PCR-introduced mutations.
Extension Speed and Cycling Time
High-fidelity polymerases often extend faster than standard Taq per kilobase, which can shorten overall cycling time for long amplicons. However, optimal cycling parameters are enzyme-specific and should follow the manufacturer's recommendations.
Cloning Method Compatibility
Some high-fidelity polymerases generate blunt-ended PCR products suited to Gibson Assembly or blunt-end cloning strategies, while others may require additional steps for TA cloning. Confirm compatibility with your intended downstream cloning method before selection.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases&mdash;combining thermostability with 3' to 5' proofreading exonuclease activity&mdash;transformed PCR from a detection and amplification tool into a precise DNA engineering platform, enabling reliable cloning, site-directed mutagenesis, and sequencing-grade amplification. The proofreading mechanism corrects misincorporated bases immediately after insertion, reducing error rates to approximately 10&minus;6 per nucleotide. Selection between high-fidelity polymerases often depends on template characteristics such as GC content, amplicon length, secondary structure, and downstream cloning requirements. High-fidelity enzymes are typically stored at &minus;20 &deg;C with matched HF buffer systems, and lot-specific Certificates of Analysis (CoAs) are available upon request. For sequencing-bound amplicons, confirm that the selected polymerase's documented error rate meets the level of sequence accuracy required, as even a 50- to 100-fold improvement over standard Taq may not be sufficient for applications demanding extremely low mutation rates across very long amplicons. Product availability reflects MBP's catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP </a>today to find high-fidelity DNA polymerase solutions for streamlining your laboratory workflows.

High-Fidelity DNA Polymerase

MegaFi Pro Fidelity DNA Polymerase

G886 MegaFi&trade; Pro Fidelity DNA Polymerase by Applied Biological Materials delivers an error rate &gt;2,000&times; lower than Taq polymerase via a Hot Start monoclonal antibody inhibitor mechanism, producing blunt-end PCR products for NGS library prep, cloning, CRISPR edit detection, and mutagenesis. ISO 13485:2016 MDSAP certified. Supplied as 400 rxn; stable 2 years at &minus;20&deg;C.

Melanocyte Medium Kit

Melanocyte Medium Kit TM047 (ABM) — low-serum, antibiotic-free medium for primary melanocyte culture. Supplied as 500 ml basal medium (TM047) + supplement mix (TM047-S). Full QC tested (sterility, pH, osmolality, endotoxin, morphology). Complete medium stable 30 days post-supplementation. Store basal at 4–8 °C; supplement at −20 °C.

MEM α, No Nucleosides Medium

MEM α, No Nucleosides Medium (TM512) by Applied Biological Materials is a 500 ml sterile basal medium with Earle's salts, L-glutamine, sodium bicarbonate (2.2 g/L), and phenol red — without ribonucleosides or deoxyribonucleosides — for DHFR-negative cell selection and general mammalian cell culture. Requires 5–10% CO₂. Store at 2–8°C, protected from light.