ABM Reagents

<h2 dir="ltr"> What Are Cas9 Enzymes?</h2>
 Cas9 is the catalytic core of the CRISPR/Cas9 editing system. Sourced from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9), recombinant Cas9 proteins are expressed and purified to high purity for direct use in ribonucleoprotein (RNP) complexes &mdash; currently the most widely recommended format for mammalian cell editing due to rapid activity onset and minimised off-target effects compared to plasmid-based delivery. Genome-editing performance depends on the appropriate nuclease format. Choose the Cas9 enzyme that aligns with your editing strategy.
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<h2 dir="ltr">What you will find:</h2>
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NLS-Tagged Cas9 and saCas9 Nucleases: Recombinant proteins of high purity designed for quick nuclear localization and strong initiation of double-strand breaks (DSBs) at specific genomic sites.
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Precision Cas9 Nickase (H840A): Tailored variants created for dual-nicking approaches to improve genomic stability and notably minimize off-target alterations.
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Lyophilized Nuclease Preparations: Nuclease-free, shelf-stable proteins that offer optimal adaptability for reconstitution and high-throughput automated CRISPR-related processes.
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Catalytically Inactive Null Mutants: "Dead" Cas9 (dCas9) proteins designed for DNA-binding research and specific epigenetic modification without changing the original sequence.
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Cas12a (asCpf1) Nucleases: Alternative CRISPR systems that focus on T-rich PAM sequences to broaden the range of spatial transcriptomics and targetable genomic areas.
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GFP-Tagged Cas9 Proteins: Fluorescently marked nucleases developed for real-time assessment of transfection efficacy and visual verification of cellular absorption.
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Endotoxin-Free Enzyme Formulations: Highly pure mixtures crucial for preserving cell viability in sensitive lentiviral applications and primary cell modifications.
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<h2 dir="ltr"> How to Choose a Cas9 Enzyme</h2>
 PAM Requirement Wild-type SpCas9 recognises any genomic locus flanked by an NGG protospacer adjacent motif (PAM), occurring roughly every 8&ndash;12 bp in mammalian genomes. SaCas9 requires NNGRRT, though engineered PAM variants (KKH, KRH, KRKH) expand targetable space. Off-Target Sensitivity High-fidelity Cas9 variants carry substitutions in the recognition lobe that reduce non-specific DNA contacts, lowering off-target cleavage while maintaining on-target activity comparable to wild-type SpCas9 &mdash; recommended for therapeutic cell engineering and base editing. Editing Mechanism Wild-type SpCas9 generates a blunt double-strand break repaired by NHEJ (indels for knockout) or HDR (precise insertions). Nickase (D10A) cuts only one strand for paired-nick HDR strategies with reduced off-target indels. dCas9 carries mutations in both nuclease domains, enabling CRISPRa/CRISPRi gene regulation without DNA cleavage. AAV Compatibility SaCas9, at approximately 1 kb smaller than SpCas9, fits a complete Cas9 + sgRNA expression cassette within the ~4.7 kb AAV packaging capacity &mdash; enabling single-vector in vivo delivery without split-vector complexity. Storage and Handling Recombinant Cas9 proteins are supplied for stability during shipping and short-term storage at &minus;20 &deg;C or &minus;80 &deg;C. Repeated freeze-thaw cycles reduce activity; aliquoting upon receipt is recommended.
<h2 dir="ltr"> Specifications Context</h2>
 An RNP is a pre-formed complex of recombinant Cas9 protein and sgRNA; once electroporated, it acts immediately and is degraded within hours, reducing off-target cleavage and avoiding immune stimulation associated with plasmid or mRNA delivery.&nbsp;Recombinant Cas9 in RNP format is validated across HEK293, HeLa, iPSCs, T cells, primary neurons, and hepatocytes, though electroporation parameters typically require cell-type-specific optimisation. Product offerings reflect MBP's catalogue as of mid-2026. 
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Cas9 Enzymes

DataSheet

MSDS

Cas9 Nickase H840A NLS Protein Functional Test Data

Cas9 Nickase H840A NLS Protein

K138 by Applied Biological Materials is spCas9 Nickase H840A NLS protein (SV40 NLS, C-terminus; H840A HNH mutation) at 10 µM (1.60 mg/mL), 40 µg / 250 pmol (25 µL) with 10× Reaction Buffer. PAM: 5′-NGG-3′. Paired double-nickase design (~40 nt recognition) for high-specificity CRISPR editing with minimal off-target effects. Store at −20°C.

Unit Quantity

Cas Type

Cas Origin

Cas Protein Marker

Concentration

Format General

Storage Buffer

Storage Condition

Caution

Shipping Condition

Cas9 Nickase H840A Protein Functional Test Data

Cas9 Nickase H840A Protein

K136 by Applied Biological Materials is a purified spCas9 H840A Nickase (HNH inactivated; RuvC1 active), supplied at 10 µM (40 µg / 250 pmol / 25 µL) with 10X Reaction Buffer. Cuts the non-complementary strand only. Use in paired-nickase strategies (with K132 D10A or a second K136 guide) for 40-nucleotide recognition specificity and minimal off-target genome editing. Store at &minus;20°C.

<h2 dir="ltr">What Are Lentiviral &amp; Vector Systems for CRISPR?</h2>
 Lentiviral vector (LV) systems deliver CRISPR/Cas9 components with the capacity to carry complex transgenes and sustain robust, long-term expression across dividing and non-dividing cell types. Unlike transient transfection, lentiviral transduction integrates the transgene into the host genome, making it the method of choice for generating stable Cas9-expressing cell lines and pooled sgRNA library screens. The right vector system influences gene delivery efficiency, expression control, and experimental success. Select the platform that best fits your research objectives.
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<h2 dir="ltr">What you will find:</h2>
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Epigenetic Modulator Lentivirus (TET1CD/DNMT3A): Tailored viral platforms created for precise DNA methylation or demethylation to investigate gene control and epigenetic changes at particular sites.
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CRISPRa &amp; CRISPRi Delivery Mechanisms (VPR/KRAB): Advanced dCas9 lentiviral vectors designed for strong transcriptional enhancement or repression while preserving the original DNA sequence.
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Cas9 Nuclease &amp; Nickase Lentiviruses: Immediately usable viral particles that ensure effective transport of double-strand break or single-strand nicking enzymes for lasting site-directed mutagenesis.
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Lentiviral Cloning &amp; Expression Vectors: Adaptable transfection-ready genetic frameworks equipped with C-terminal tags and enhanced promoters for the creation of tailored guide RNA and effector proteins.
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Non-Viral Cas9 Vector Options: High-quality plasmid-based systems intended for temporary expression in scenarios where viral incorporation is not needed.
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High-Titer Packaging Solutions: Viral preparations safe from endotoxins, enhanced for optimal transduction efficiency in delicate therapeutic candidate evaluation and disease modeling.
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<h2 dir="ltr"> How to Choose Lentiviral/Vector CRISPR Systems</h2>
 Packaging Generation Second- and third-generation packaging designs progressively remove viral accessory genes (nef, vif, vpr, vpu) for enhanced biosafety. Third-generation systems split packaging components across two plasmids and eliminate the Tat transactivator, producing replication-incompetent particles suitable for BSL-2 settings. CRISPR-Specific Format Cas9 lentiviral plasmids provide stable Cas9 integration; pre-linearised sgRNA lentiviral plasmids are ready for guide sequence insertion; dox-inducible (Tet-On) Cas9 systems allow temporally controlled editing. Paired Cas9 and sgRNA vectors generate inducible stable editing cell lines. Transient vs. Stable Needs For experiments requiring transient editing without permanent genomic modification, all-in-one or dual-plasmid Cas9/sgRNA transfection systems (lipid-based or electroporation) are appropriate. For stable, heritable expression, lentiviral integration is required. Cell Type Lentiviral vectors transduce both dividing and non-dividing cells, including post-mitotic neurons and macrophages &mdash; applications that gamma-retroviral vectors cannot support. Screening Scale Pooled sgRNA libraries transduced at low MOI enable genome-wide functional screens; stable lentiviral Cas9 cell lines, generated once and banked, provide a consistent starting point for multiple guide RNA screen iterations. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral and vector systems support pooled genome-wide CRISPR knockout screens, stable Cas9 cell line generation, inducible gene editing models, hard-to-transfect primary cell engineering, and CAR-T cell development workflows. Lentiviral plasmids contain LTRs prone to recombination in standard E. coli strains &mdash; recombination-resistant strains such as Stbl3 are recommended for plasmid propagation. Integrase-deficient lentiviral vectors (IDLVs) or RNP electroporation are preferred where insertional mutagenesis risk must be minimised, such as therapeutic cell engineering. For research-scale work where stable integration is the goal, third-generation lentiviral systems remain the standard choice given their established safety record and broad cell-type compatibility across academic and industry labs. Labs new to lentiviral work often start with a well-characterised packaging system and a small pilot transduction before scaling to a full screen or stable line generation campaign.
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quote for the perfect Lentivirus system tailored to your research needs.

Vector & Lentivirus Systems

Lentivirus Systems

Restriction Enzyme Analysis of K005

Cas9 Nickase Lentiviral Vector

K005 Cas9 Nickase Lentiviral Vector by Applied Biological Materials is a 10 µg plasmid DNA lentiviral transfer vector encoding Cas9 Nickase (D10A, S. pyogenes) with N-terminal SV40 NLS for in-house packaging of high-specificity single-strand-nick CRISPR editing lentivirus. For pre-packaged particles, see K006. Store at &minus;20&deg;C or below.

Vector

Note

QC - Lot. No. VH7954

Cas9 Nickase Lentivirus

K006 Cas9 Nickase Lentivirus by Applied Biological Materials expresses Cas9 Nickase (D10A mutation) with N-terminal SV40 NLS for single-strand nick-based CRISPR editing with reduced off-target effects. Supplied at 107 IU/mL as 3 &times; 250 µl; shipped on dry ice, store at &minus;80&deg;C.

Titer

<h2 dir="ltr">What Are AAV and Adenovirus CRISPR Delivery Systems?</h2>
 AAV and adenovirus-based delivery systems exploit the natural cell-entry machinery of these viruses to deliver Cas9 and guide RNA cargo into cells that resist conventional transfection &mdash; including primary cells, stem cells, neurons, and in vivo tissues. AAV is the most widely used viral vector for CRISPR-based gene editing, valued for high transduction efficiency, low immunogenicity, and an established safety profile in human gene therapy. The right viral delivery system influences transduction efficiency, expression profile, and genome-editing outcomes. AAV and adenoviral vectors offer distinct advantages depending on your research goals.
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="17k5oh4" data-start="0" data-end="142">Adenoviral Cas9 &amp; Nickase Nucleases: Viral delivery systems for rapid, transient Cas9 or nickase expression without genomic integration.</li>
<li data-section-id="12kolej" data-start="144" data-end="280">AAV6 &amp; AAV8 Viral Vectors: Serotype-specific AAV vectors optimized for transduction of hematopoietic stem cells and liver tissues.</li>
<li data-section-id="81toc4" data-start="282" data-end="412">saCas9 AAV Serotypes (1&ndash;10): Diverse AAV serotypes designed to support targeted cellular tropism across CRISPR applications.</li>
<li data-section-id="18h5j1a" data-start="414" data-end="554">Ampicillin-Selected AAV Vectors: AAV vector backbones containing ampicillin resistance markers for cloning and transformant selection.</li>
<li data-section-id="xfx9g1" data-start="556" data-end="697" data-is-last-node="">High-Titer Viral Particles: Purified viral preparations optimized to maintain genomic integrity and minimize endotoxin-related effects.</li>
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<h2 dir="ltr"> How to Choose AAV/Adenovirus CRISPR Delivery</h2>
 Genome Capacity Requirements AAV's ~4.7 kb packaging capacity is too small for full-length SpCas9 (~4.2 kb) plus a promoter and guide RNA. Split-vector systems (Cas9 across two AAV particles) or compact Cas9 orthologs such as SaCas9 (~3.2 kb) address this limit. Adenovirus offers 34&ndash;43 kb capacity for larger cargo. Serotype and Tissue Tropism AAV serotypes differ in their ability to transduce specific cell types and tissues, making serotype selection an important part of CRISPR delivery design. The optimal serotype depends on the target tissue, model system, administration route, and experimental goals. MBP offers saCas9 AAV viruses in multiple serotypes (AAV1&ndash;AAV11) to support a range of research applications. Cell Division Status Both AAV and adenovirus transduce dividing and non-dividing cells, making them suitable for primary neurons, hepatocytes, and other post-mitotic populations that resist standard transfection. Immunogenicity Considerations AAV has low immunogenicity and minimal integration risk, supporting widespread pre-clinical and clinical in vivo use. Adenovirus offers higher initial transduction efficiency and larger cargo capacity but generates a stronger immune response, which can limit in vivo utility. Packaging System Helper-free packaging systems eliminate the need for replication-competent helper virus, designed for BSL-2 containment &mdash; though institutional biosafety committee (IBC) approval is required before starting viral vector work. 
<h2 dir="ltr"> Specifications Context</h2>
 AAV and adenoviral CRISPR delivery systems support retinal gene therapy models, CNS editing studies, liver-targeted in vivo CRISPR, muscle cell engineering, primary immune cell editing, iPSC-derived cell modification, and genome-modified animal model generation. Capsid engineering through directed-evolution platforms continues to expand AAV tropism options, including enhanced CNS penetration following systemic delivery, an active area of pre-clinical research relevant to labs planning long-term in vivo programs. All recombinant AAV production and adenoviral vector work requires IBC approval at BSL-2 containment in most jurisdictions; MBP can provide biosafety classification documentation. Product availability reflects MBP's catalogue as of mid-2026. 
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">team at MBP Inc</a>. now to ask for a quote for a suitable AAV or Adenovirus system for your lab.

AAV/Adenovirus Systems

Cas9 Nuclease Adenovirus

K004 by Applied Biological Materials is a standard-titer adenovirus (1 ml at 106 pfu/ml) expressing spCas9 nuclease with dual NLS (N-terminal SV40 + C-terminal Nucleoplasmin) for efficient nuclear import. Non-integrating transient Cas9 delivery for CRISPR editing in primary and post-mitotic cells. Separate sgRNA (5'-NGG PAM) required. Store at &minus;80°C in small aliquots.

Cas9 Nuclease GFP NLS Protein

K148 by Applied Biological Materials is Cas9 Nuclease GFP NLS Protein — high-concentration spCas9-eGFP-SV40 NLS. 10 µM (1.88 mg/mL); 47 µg/250 pmol/25 µL; 10× Buffer. GFP enables visual Cas9 delivery confirmation, FACS sorting, and clearance tracking. NGG PAM. Store at −20°C.

K048 by Applied Biological Materials is Cas9 Nuclease GFP NLS Protein — spCas9-eGFP-SV40 NLS. 1,000 nM (188 µg/mL); 9.5 µg/50 pmol/50 µL; 10× Buffer. GFP for delivery confirmation, FACS sorting, Cas9 clearance tracking. NGG PAM. Standard-scale entry-level format. High-concentration format: K148. Store at −20°C.

Cas9 Nuclease Lentiviral Vector

K002 Cas9 Nuclease Lentiviral Vector by Applied Biological Materials is a 10 µg plasmid DNA lentiviral transfer vector expressing S. pyogenes Cas9 with dual NLS (SV40 + Nucleoplasmin) and Puromycin resistance, for in-house lentiviral packaging or direct Cas9 delivery in CRISPR gene editing applications. Store at &minus;20&deg;C or below.

QC - Lot. No. VH7955

Cas9 Nuclease Lentivirus

K003 Cas9 Nuclease Lentivirus by Applied Biological Materials expresses S. pyogenes Cas9 with dual NLS tags (SV40 + Nucleoplasmin) and Puromycin resistance at 107 IU/mL for stable CRISPR gene editing in mammalian cells. Supplied as 3 &times; 250 &micro;l; store at &minus;80&deg;C.

Cas9 Nuclease NLS Protein Functional Test Report

Cas9 Nuclease NLS Protein

K030 by Applied Biological Materials is a purified spCas9 nuclease with a C-terminal SV40 NLS, supplied at 1000 nM (8.0 µg / 50 pmol / 50 µL) with 10X Reaction Buffer. For RNP-based CRISPR genome editing, NHEJ gene disruption, and HDR knock-in in mammalian cells. Active nuclear import via NLS. Store at &minus;20°C.

K130 by Applied Biological Materials is recombinant spCas9 Nuclease NLS protein (SV40 NLS, C-terminus) at 10 µM (1.60 mg/mL), supplied as 40 µg / 250 pmol (25 µL) with 10× Reaction Buffer. PAM: 5′-NGG-3′. No GFP. For RNP-based CRISPR gene knockout and HDR in primary cells. Store at −20°C.

Cas9 Nuclease NLS Protein (Lyophilized)

K151 by Applied Biological Materials is a lyophilized spCas9 nuclease with a C-terminal SV40 NLS for active nuclear import, supplied as 200 µg (1.25 nmol) with 10X Reaction Buffer. Resuspend in 125 µL nuclease-free water. Suitable for RNP-based genome editing, NHEJ-mediated gene disruption, and HDR knock-in in mammalian cells. Store at &minus;20°C.