ABM Reagents

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
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Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
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<h2 data-path-to-node="5">What you will find:</h2>
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Tissue-specific primary cell culture medium kits
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Specialized growth media for endothelial and epithelial cells
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Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
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Differentiation media for progenitor and stem cells&nbsp;
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Serum-free bioprocessing and cryopreservation reagents
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Targeted fibroblast, smooth muscle, and myocyte growth kits
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<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
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Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
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<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
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Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Bronchial Endothelial Cell Growth Medium

Bronchial Endothelial Cell Growth Medium TM092 (ABM) — 500 ml proprietary ready-to-use medium for primary bronchial endothelial cell expansion. Sterility tested, fully QC validated. No supplementation required. Store 2–8 °C. RUO.

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TM044 - MSDS

Flyer

Bronchial Epithelial Medium Kit

TM044 by Applied Biological Materials is a low-serum Bronchial Epithelial Medium Kit (500 mL basal + antibiotic-free supplement) supporting bronchial epithelial cell culture with lot-specific sterility, pH, osmolality, and endotoxin QC testing.

<h2 dir="ltr">What Are LAMP Polymerases?</h2>
 The defining enzyme of loop-mediated isothermal amplification is Bst DNA polymerase, isolated from Geobacillus stearothermophilus (formerly Bacillus stearothermophilus). Its high strand-displacement activity allows it to synthesise new DNA strands while displacing the existing complementary strand, without requiring thermal denaturation, the property that makes isothermal amplification possible. Choose standard Bst DNA polymerase for DNA-template LAMP; choose engineered Bst variants (Bst 2.0, Bst 3.0, or lyo-ready formats) for RT-LAMP applications requiring combined reverse transcriptase activity, faster reaction speed, or room-temperature-stable formats. 
<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="6ukg4n" data-start="2051" data-end="2199">Strong strand-displacement DNA synthesis using Bst DNA polymerase variants, enabling rapid isothermal amplification without thermal cycling.</li>
<li data-section-id="7ycop5" data-start="2200" data-end="2330">High-efficiency Phi29 DNA polymerase systems supporting whole-genome amplification through processive strand displacement.</li>
<li data-section-id="18wphsf" data-start="2331" data-end="2463">Enzyme formats optimised for fast, constant-temperature nucleic acid amplification across LAMP and related isothermal methods.</li>
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<h2 dir="ltr">How to Choose a LAMP Polymerase</h2>
 Strand-Displacement Activity Bst DNA polymerase's large fragment (Bst LF) provides the core strand-displacement activity that enables LAMP, operating at a temperature optimum near 65 &deg;C -- this activity allows continuous synthesis and primer annealing on single-stranded regions generated during amplification, without thermal cycling. Reverse Transcription Capability Engineered variants such as Bst 3.0 may offer enhanced reverse transcription capability, enabling RT-LAMP workflows by supporting RNA-to-cDNA conversion within the same isothermal reaction environment. Reaction Speed Optimised Bst formulations can support rapid amplification in LAMP reactions, with performance depending on primer design, template quality, and reaction conditions. Format: Liquid vs. Lyophilised Lyo-ready (lyophilised) formats support room-temperature shipping and storage, relevant for field-deployable or point-of-need applications where cold-chain logistics are impractical. Standard liquid formulations require frozen storage but may offer cost advantages for laboratory-based use. Pairing with Reverse Transcriptase For RT-LAMP using polymerases without intrinsic RT activity, pairing with a reverse transcriptase whose temperature optimum overlaps with the Bst polymerase's working temperature (commonly 60-65 &deg;C) helps maintain the isothermal format -- engineered high-thermostability reverse transcriptases are better suited to this than standard AMV or M-MLV enzymes with lower temperature optima. 
<h2 dir="ltr"> Specifications Context</h2>
 Bst DNA polymerase large fragment drives LAMP through strand-displacement activity at its temperature optimum near 65 &deg;C; because LAMP uses this single-temperature synthesis instead of thermal denaturation, the enzyme's strand-displacement capability -- rather than thermostability across a wide cycling range as with Taq -- is the key performance attribute. When combining AMV or M-MLV reverse transcriptases (with lower native temperature optima) with Bst polymerase for RT-LAMP, the temperature mismatch can require a two-temperature protocol, undermining the isothermal format; this has driven engineering efforts producing polymerases such as Bst 3.0 with intrinsic reverse transcriptase activity functioning at the Bst temperature optimum. Comparative testing has shown lyo-ready Bst DNA polymerase formulations can provide significantly higher RT-LAMP reaction speed than some other commercially available Bst polymerases when tested with the same RNA targets and primer sets. LAMP polymerases are typically stored at &minus;20 &deg;C (liquid formats) or at room temperature (lyo-ready formats per product specification); lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to find polymerases suited to your LAMP and isothermal amplification workflows.

Polymerases

DataSheet

MSDS

Bst DNA Polymerase

G880 by Applied Biological Materials is Bst DNA Polymerase Large Fragment from Geobacillus stearothermophilus. Strand-displacement + 5′→3′ synthesis; no exonuclease. Optimal at 60–65°C. For LAMP, SDA, WGA, and isothermal nucleic acid amplification. Store at −20°C.

Antibiotics

Carbenicillin

Carbenicillin G054 — 1.0 g β-lactam antibiotic powder. More stable than ampicillin; greatly reduces satellite colonies on selection plates. For bla-gene plasmid selection in E. coli at 50–100 µg/ml. Working concentration determined by end user. Store −20 °C, protect from light, up to 1 year.

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<h2 dir="ltr"> What Are Cas9 Enzymes?</h2>
 Cas9 is the catalytic core of the CRISPR/Cas9 editing system. Sourced from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9), recombinant Cas9 proteins are expressed and purified to high purity for direct use in ribonucleoprotein (RNP) complexes &mdash; currently the most widely recommended format for mammalian cell editing due to rapid activity onset and minimised off-target effects compared to plasmid-based delivery. Genome-editing performance depends on the appropriate nuclease format. Choose the Cas9 enzyme that aligns with your editing strategy.
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<h2 dir="ltr">What you will find:</h2>
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NLS-Tagged Cas9 and saCas9 Nucleases: Recombinant proteins of high purity designed for quick nuclear localization and strong initiation of double-strand breaks (DSBs) at specific genomic sites.
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Precision Cas9 Nickase (H840A): Tailored variants created for dual-nicking approaches to improve genomic stability and notably minimize off-target alterations.
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Lyophilized Nuclease Preparations: Nuclease-free, shelf-stable proteins that offer optimal adaptability for reconstitution and high-throughput automated CRISPR-related processes.
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Catalytically Inactive Null Mutants: "Dead" Cas9 (dCas9) proteins designed for DNA-binding research and specific epigenetic modification without changing the original sequence.
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Cas12a (asCpf1) Nucleases: Alternative CRISPR systems that focus on T-rich PAM sequences to broaden the range of spatial transcriptomics and targetable genomic areas.
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GFP-Tagged Cas9 Proteins: Fluorescently marked nucleases developed for real-time assessment of transfection efficacy and visual verification of cellular absorption.
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Endotoxin-Free Enzyme Formulations: Highly pure mixtures crucial for preserving cell viability in sensitive lentiviral applications and primary cell modifications.
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<h2 dir="ltr"> How to Choose a Cas9 Enzyme</h2>
 PAM Requirement Wild-type SpCas9 recognises any genomic locus flanked by an NGG protospacer adjacent motif (PAM), occurring roughly every 8&ndash;12 bp in mammalian genomes. SaCas9 requires NNGRRT, though engineered PAM variants (KKH, KRH, KRKH) expand targetable space. Off-Target Sensitivity High-fidelity Cas9 variants carry substitutions in the recognition lobe that reduce non-specific DNA contacts, lowering off-target cleavage while maintaining on-target activity comparable to wild-type SpCas9 &mdash; recommended for therapeutic cell engineering and base editing. Editing Mechanism Wild-type SpCas9 generates a blunt double-strand break repaired by NHEJ (indels for knockout) or HDR (precise insertions). Nickase (D10A) cuts only one strand for paired-nick HDR strategies with reduced off-target indels. dCas9 carries mutations in both nuclease domains, enabling CRISPRa/CRISPRi gene regulation without DNA cleavage. AAV Compatibility SaCas9, at approximately 1 kb smaller than SpCas9, fits a complete Cas9 + sgRNA expression cassette within the ~4.7 kb AAV packaging capacity &mdash; enabling single-vector in vivo delivery without split-vector complexity. Storage and Handling Recombinant Cas9 proteins are supplied for stability during shipping and short-term storage at &minus;20 &deg;C or &minus;80 &deg;C. Repeated freeze-thaw cycles reduce activity; aliquoting upon receipt is recommended.
<h2 dir="ltr"> Specifications Context</h2>
 An RNP is a pre-formed complex of recombinant Cas9 protein and sgRNA; once electroporated, it acts immediately and is degraded within hours, reducing off-target cleavage and avoiding immune stimulation associated with plasmid or mRNA delivery.&nbsp;Recombinant Cas9 in RNP format is validated across HEK293, HeLa, iPSCs, T cells, primary neurons, and hepatocytes, though electroporation parameters typically require cell-type-specific optimisation. Product offerings reflect MBP's catalogue as of mid-2026. 
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Reach out to the supportive <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quotation for the perfect Cas9 enzyme for your study.

Cas9 Enzymes

Cas12a (asCpf1) Nuclease NLS Protein

K188 by Applied Biological Materials is AsCas12a (AsCpf1) Nuclease NLS Protein. TTTV PAM; crRNA-only guide (no tracrRNA); staggered 5-nt 5′ overhang DSB; ssDNA trans-cleavage (DETECTR). NLS for nuclear import. No GFP. For RNP editing at AT-rich loci and multiplexed CRISPR. Store at −20°C.

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Cas12a (fnCpf1) Nuclease NLS Protein

K187 by Applied Biological Materials is Cas12a (fnCpf1) Nuclease NLS Protein from Francisella novicida (380 µg / 2.5 nmol / 250 µl). Uses a single crRNA (no tracrRNA), TTTN PAM, and produces 5'-overhang staggered cuts. Intrinsic RNase activity enables multiplex pre-crRNA processing. Deliver as RNP by electroporation or lipofection for CRISPR genome editing at AT-rich or TTTN PAM sites. Store at &minus;80°C.

Cas9 D10A Nickase GFP NLS Protein

K149 by Applied Biological Materials is a purified spCas9 D10A Nickase with C-terminal GFP and SV40 NLS fusions, supplied at 10 µM (47 µg / 250 pmol / 25 µL) with 10X Reaction Buffer. HNH-active nickase cuts the complementary strand only; use in paired-guide strategies for 40-nucleotide specificity. GFP enables delivery confirmation and Cas9 clearance tracking. Store at &minus;20°C.

<h2 dir="ltr">What Are Lentiviral &amp; Vector Systems for CRISPR?</h2>
 Lentiviral vector (LV) systems deliver CRISPR/Cas9 components with the capacity to carry complex transgenes and sustain robust, long-term expression across dividing and non-dividing cell types. Unlike transient transfection, lentiviral transduction integrates the transgene into the host genome, making it the method of choice for generating stable Cas9-expressing cell lines and pooled sgRNA library screens. The right vector system influences gene delivery efficiency, expression control, and experimental success. Select the platform that best fits your research objectives.
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<h2 dir="ltr">What you will find:</h2>
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Epigenetic Modulator Lentivirus (TET1CD/DNMT3A): Tailored viral platforms created for precise DNA methylation or demethylation to investigate gene control and epigenetic changes at particular sites.
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CRISPRa &amp; CRISPRi Delivery Mechanisms (VPR/KRAB): Advanced dCas9 lentiviral vectors designed for strong transcriptional enhancement or repression while preserving the original DNA sequence.
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Cas9 Nuclease &amp; Nickase Lentiviruses: Immediately usable viral particles that ensure effective transport of double-strand break or single-strand nicking enzymes for lasting site-directed mutagenesis.
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Lentiviral Cloning &amp; Expression Vectors: Adaptable transfection-ready genetic frameworks equipped with C-terminal tags and enhanced promoters for the creation of tailored guide RNA and effector proteins.
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Non-Viral Cas9 Vector Options: High-quality plasmid-based systems intended for temporary expression in scenarios where viral incorporation is not needed.
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High-Titer Packaging Solutions: Viral preparations safe from endotoxins, enhanced for optimal transduction efficiency in delicate therapeutic candidate evaluation and disease modeling.
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<h2 dir="ltr"> How to Choose Lentiviral/Vector CRISPR Systems</h2>
 Packaging Generation Second- and third-generation packaging designs progressively remove viral accessory genes (nef, vif, vpr, vpu) for enhanced biosafety. Third-generation systems split packaging components across two plasmids and eliminate the Tat transactivator, producing replication-incompetent particles suitable for BSL-2 settings. CRISPR-Specific Format Cas9 lentiviral plasmids provide stable Cas9 integration; pre-linearised sgRNA lentiviral plasmids are ready for guide sequence insertion; dox-inducible (Tet-On) Cas9 systems allow temporally controlled editing. Paired Cas9 and sgRNA vectors generate inducible stable editing cell lines. Transient vs. Stable Needs For experiments requiring transient editing without permanent genomic modification, all-in-one or dual-plasmid Cas9/sgRNA transfection systems (lipid-based or electroporation) are appropriate. For stable, heritable expression, lentiviral integration is required. Cell Type Lentiviral vectors transduce both dividing and non-dividing cells, including post-mitotic neurons and macrophages &mdash; applications that gamma-retroviral vectors cannot support. Screening Scale Pooled sgRNA libraries transduced at low MOI enable genome-wide functional screens; stable lentiviral Cas9 cell lines, generated once and banked, provide a consistent starting point for multiple guide RNA screen iterations. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral and vector systems support pooled genome-wide CRISPR knockout screens, stable Cas9 cell line generation, inducible gene editing models, hard-to-transfect primary cell engineering, and CAR-T cell development workflows. Lentiviral plasmids contain LTRs prone to recombination in standard E. coli strains &mdash; recombination-resistant strains such as Stbl3 are recommended for plasmid propagation. Integrase-deficient lentiviral vectors (IDLVs) or RNP electroporation are preferred where insertional mutagenesis risk must be minimised, such as therapeutic cell engineering. For research-scale work where stable integration is the goal, third-generation lentiviral systems remain the standard choice given their established safety record and broad cell-type compatibility across academic and industry labs. Labs new to lentiviral work often start with a well-characterised packaging system and a small pilot transduction before scaling to a full screen or stable line generation campaign.
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quote for the perfect Lentivirus system tailored to your research needs.

Vector & Lentivirus Systems

Lentivirus Systems

Cas9 Double Mutant Lentiviral Vector

K012 is a lentiviral vector expressing catalytically dead Cas9 (dCas9) with D10A and H840A mutations that abolish both nuclease and nickase activity. Supplied as 10 µg, it enables stable dCas9 expression for CRISPRi, CRISPRa, and chromatin imaging applications when paired with target-specific sgRNAs.

Vector

Cas9 Double Mutant Lentivirus

K013 Cas9 Double Mutant Lentivirus (dCas9) by Applied Biological Materials expresses catalytically dead Cas9 (D10A + H840A; no nuclease or nickase activity) for sgRNA-guided DNA binding without cleavage, enabling CRISPRi, dead-Cas9 controls, and stable dCas9 cell lines for effector delivery. 107 IU/mL; 3 &times; 250 µl; shipped dry ice, store &minus;80&deg;C.

Titer

<h2 dir="ltr">What Are AAV and Adenovirus CRISPR Delivery Systems?</h2>
 AAV and adenovirus-based delivery systems exploit the natural cell-entry machinery of these viruses to deliver Cas9 and guide RNA cargo into cells that resist conventional transfection &mdash; including primary cells, stem cells, neurons, and in vivo tissues. AAV is the most widely used viral vector for CRISPR-based gene editing, valued for high transduction efficiency, low immunogenicity, and an established safety profile in human gene therapy. The right viral delivery system influences transduction efficiency, expression profile, and genome-editing outcomes. AAV and adenoviral vectors offer distinct advantages depending on your research goals.
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<h2 dir="ltr">What you will find:</h2>
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<li data-section-id="17k5oh4" data-start="0" data-end="142">Adenoviral Cas9 &amp; Nickase Nucleases: Viral delivery systems for rapid, transient Cas9 or nickase expression without genomic integration.</li>
<li data-section-id="12kolej" data-start="144" data-end="280">AAV6 &amp; AAV8 Viral Vectors: Serotype-specific AAV vectors optimized for transduction of hematopoietic stem cells and liver tissues.</li>
<li data-section-id="81toc4" data-start="282" data-end="412">saCas9 AAV Serotypes (1&ndash;10): Diverse AAV serotypes designed to support targeted cellular tropism across CRISPR applications.</li>
<li data-section-id="18h5j1a" data-start="414" data-end="554">Ampicillin-Selected AAV Vectors: AAV vector backbones containing ampicillin resistance markers for cloning and transformant selection.</li>
<li data-section-id="xfx9g1" data-start="556" data-end="697" data-is-last-node="">High-Titer Viral Particles: Purified viral preparations optimized to maintain genomic integrity and minimize endotoxin-related effects.</li>
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<h2 dir="ltr"> How to Choose AAV/Adenovirus CRISPR Delivery</h2>
 Genome Capacity Requirements AAV's ~4.7 kb packaging capacity is too small for full-length SpCas9 (~4.2 kb) plus a promoter and guide RNA. Split-vector systems (Cas9 across two AAV particles) or compact Cas9 orthologs such as SaCas9 (~3.2 kb) address this limit. Adenovirus offers 34&ndash;43 kb capacity for larger cargo. Serotype and Tissue Tropism AAV serotypes differ in their ability to transduce specific cell types and tissues, making serotype selection an important part of CRISPR delivery design. The optimal serotype depends on the target tissue, model system, administration route, and experimental goals. MBP offers saCas9 AAV viruses in multiple serotypes (AAV1&ndash;AAV11) to support a range of research applications. Cell Division Status Both AAV and adenovirus transduce dividing and non-dividing cells, making them suitable for primary neurons, hepatocytes, and other post-mitotic populations that resist standard transfection. Immunogenicity Considerations AAV has low immunogenicity and minimal integration risk, supporting widespread pre-clinical and clinical in vivo use. Adenovirus offers higher initial transduction efficiency and larger cargo capacity but generates a stronger immune response, which can limit in vivo utility. Packaging System Helper-free packaging systems eliminate the need for replication-competent helper virus, designed for BSL-2 containment &mdash; though institutional biosafety committee (IBC) approval is required before starting viral vector work. 
<h2 dir="ltr"> Specifications Context</h2>
 AAV and adenoviral CRISPR delivery systems support retinal gene therapy models, CNS editing studies, liver-targeted in vivo CRISPR, muscle cell engineering, primary immune cell editing, iPSC-derived cell modification, and genome-modified animal model generation. Capsid engineering through directed-evolution platforms continues to expand AAV tropism options, including enhanced CNS penetration following systemic delivery, an active area of pre-clinical research relevant to labs planning long-term in vivo programs. All recombinant AAV production and adenoviral vector work requires IBC approval at BSL-2 containment in most jurisdictions; MBP can provide biosafety classification documentation. Product availability reflects MBP's catalogue as of mid-2026. 
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">team at MBP Inc</a>. now to ask for a quote for a suitable AAV or Adenovirus system for your lab.

AAV/Adenovirus Systems

Cas9 Nickase Adenovirus

K007 by Applied Biological Materials is a standard-titer Cas9 Nickase D10A adenovirus (1.0 ml at 106 pfu/ml, Dry Ice) with N-terminal SV40 NLS. D10A RuvC inactivation creates single-strand nicks; paired offset sgRNAs convert nicks to a functional DSB with reduced off-target editing compared to standard Cas9 nuclease. Non-integrating, transient expression. Two sgRNAs (offset 0–100 bp) required for paired-nicking strategy. Store at &minus;80°C.

Cas9 Nickase D10A NLS Protein Functional Test Data

Cas9 Nickase D10A NLS Protein

K134 by Applied Biological Materials is Cas9 Nickase D10A NLS Protein — spCas9 D10A (RuvC1 inactivated; HNH intact); nicks non-complementary strand. SV40 NLS. 10 µM (1.60 mg/mL); 40 µg/250 pmol/25 µL; 10× Buffer. For paired double-nickase high-specificity editing and HDR. No GFP. Store at −20°C.

Cas9 Nickase D10A Protein Functional Test Report

Cas9 Nickase D10A Protein

K132 by Applied Biological Materials is a purified spCas9 Nickase with the D10A RuvC1 domain mutation, supplied at 10 µM (250 pmol / 25 µL) with 10X Reaction Buffer. Requires paired guide RNAs for DSB induction, effectively doubling target specificity to 40 nucleotides and minimizing off-target genome editing effects. Store at &minus;20°C.