PCR Reagents & Kits

<h2>What Is Reverse Transcriptase?</h2>
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Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
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<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
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<h2>How to Choose a Reverse Transcriptase</h2>
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Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
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<h2>Specifications Context</h2>
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Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

Reverse Transcriptase

DataSheet

MSDS

PCR Product Guide

OneScript Hot Reverse Transcriptase

G593 OneScript&reg; Hot Reverse Transcriptase by Applied Biological Materials is a thermostable (60&ndash;72&deg;C), RNase H-deficient M-MLV RT derivative for high-yield cDNA synthesis from difficult, low-abundance, or partially degraded RNA, formulated with oxidation-resistant RNaseOFF inhibitor. ISO 13485:2016 MDSAP certified; 100 rxn; store at &minus;20&deg;C.

Unit Quantity

Storage Condition

Shipping Condition

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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cDNA Synthesis Kits

OneScript Hot RT cDNA Synthesis Kit

G594 OneScript&reg; Hot cDNA Synthesis Kit by Applied Biological Materials is a complete 100 rxn kit for cDNA synthesis at 60&ndash;72&deg;C using thermostable, RNase H-deficient OneScript&reg; Hot RT with RNaseOFF inhibitor, dNTPs, Oligo(dT), and Random Primers. The first kit optimized for 60&deg;C cDNA synthesis. ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

OneScript Plus cDNA Synthesis Kit

G236 by Applied Biological Materials is the OneScript&reg; Plus cDNA Synthesis Kit (100 rxn) built on an engineered RNase H-null, thermally stable M-MLV RT. Synthesizes cDNA up to 12 kb at 50–55°C, with RNaseOFF inhibitor stable at &lt;1 mM DTT. Three primer options (oligo(dT), random, gene-specific). ISO 13485:2016 MDSAP Certified. Equivalent to G236EU. Store at &minus;20°C.

Application

OneScript Plus Reverse Transcriptase

G237 OneScript&reg; Plus Reverse Transcriptase by Applied Biological Materials is a thermostable (50&ndash;55&deg;C), RNase H-deficient M-MLV RT for cDNA synthesis up to 15 kb in 10&ndash;15 minutes, formulated with RNaseOFF inhibitor. Supplied as 100 rxn (100 µl enzyme + 400 µl 5X RT Buffer). ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

<h2 dir="ltr">What Is a Mycoplasma PCR Detection Kit?</h2>
 A Mycoplasma PCR detection kit provides primers, master mix, ultrapure water, and a positive control for rapid PCR-based screening of cell culture samples for Mycoplasma contamination. Because Mycoplasma species lack a cell wall and do not produce visible turbidity or media discoloration, contamination frequently goes undetected without periodic molecular testing, making these kits a standard component of cell culture quality assurance programmes. Run a Mycoplasma PCR detection kit periodically on actively maintained cell lines and before banking, sharing, or publishing results from a cell line as routine quality assurance against a contaminant that produces no visible signs. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Detection &amp; Elimination Kits: All-encompassing systems such as Mycoplasma Pro crafted to accurately identify contamination while also enabling complete eradication of infected cultures.
</li>
<li dir="ltr" aria-level="1">
PCR Detection Kits: Standardized, validated reagents that provide a quick and economical solution for regular laboratory testing and quality assurance.
</li>
<li dir="ltr" aria-level="1">
Mycoplasma gDNA Kits: Tailored genomic DNA extraction solutions specifically designed for isolating low-abundance mycoplasma templates from intricate biological specimens.
</li>
</ul>
<h2 dir="ltr"> How to Choose a Mycoplasma PCR Detection Kit</h2>
 Species/Strain Coverage Kits vary in the breadth of Mycoplasma genera and species detected -- some formats detect over 200 strains, while enhanced ("Pro"-type) formats may extend coverage to 300+ strains, targeting conserved regions shared across common contaminant species such as M. arginini, M. hyorhinis, M. orale, and others. Sample Preparation Requirements Many Mycoplasma PCR detection kits allow direct addition of cell culture media to the PCR reaction without a separate DNA extraction step, simplifying the workflow and reducing time-to-result -- confirm whether your chosen kit requires extraction or supports direct sample addition. Internal Control Availability Enhanced kit formats may include an internal amplification control, providing additional confidence that a negative result reflects true absence of contamination rather than a failed or inhibited PCR reaction -- particularly valuable for samples with potential PCR inhibitors. Detection Sensitivity Standard kits can detect contamination at typical levels found in actively growing contaminated cultures; ultra-sensitive qPCR-based Mycoplasma detection formats (a related but distinct product type) can detect as few as 10 genome copies directly from media for earlier-stage contamination detection. Result Interpretation A positive result is typically indicated by a PCR product in a defined size range (commonly in the 260-550 bp range depending on the specific primer design), distinguishable from the positive control band; a clean no-template control validates assay specificity for each run. 
<h2 dir="ltr"> Specifications Context</h2>
 Mycoplasma PCR detection kits use primers highly specific to conserved rDNA regions across Mycoplasma genomes, enabling a single primer mix to detect dozens to hundreds of species and strains -- including commonly encountered laboratory contaminants - in one reaction. The streamlined workflow (combine sample with master mix and primer mix, run PCR, analyse by gel electrophoresis) typically delivers results within 1-2 hours, in contrast to culture-based Mycoplasma testing methods that can take days to weeks. A positive result appears as a distinct PCR product within the kit's expected size range, while the included positive control confirms the reaction performed correctly even when test samples are negative. Routine screening intervals vary by institutional policy, but testing before banking a new cell line, after receiving cells from an external source, and periodically during long-term culture are common checkpoints. Mycoplasma PCR detection kits are typically stored at &minus;20 &deg;C; positive controls are included with the kit. 
Safeguard the integrity of your culture&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">reach out to the MBP team</a> now for a quote on our expert Mycoplasma detection services.

Mycoplasma PCR Detection Kit

Flyer

G238-List of Detectable Mycoplasma Species/Strains

PCR Mycoplasma Detection Kit

PCR Mycoplasma Detection Kit (G238) by Applied Biological Materials is a 100-reaction kit detecting 200+ Mycoplasma strains in under 2 hours with no DNA isolation. Includes BlasTaq™ MasterMix with gel loading dye, Primer Mix, Positive Control, and Nuclease-Free H₂O. ISO 13485:2016 MDSAP certified. Outperforms Sigma and Lonza kits (Russell et al., 2020). Store at –20°C; stable 2 years.

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
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Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

Taq PCR Master Mixes

PCR Sure™ Kit

G065 by Applied Biological Materials is the PCR Sure&trade; Kit (8 × 10 rxn) — eight distinct PCR formulations for systematic troubleshooting of difficult, failed, or non-specific PCR amplifications. Addresses GC-rich templates, long amplicons, secondary structure, and primer non-specificity. Add template + primers to each formulation; compare results to identify the optimal condition. Consult the PCR Product Guide to scale up with the best-matching abm PCR reagent.

<h2 dir="ltr">What Are LAMP Polymerases?</h2>
 The defining enzyme of loop-mediated isothermal amplification is Bst DNA polymerase, isolated from Geobacillus stearothermophilus (formerly Bacillus stearothermophilus). Its high strand-displacement activity allows it to synthesise new DNA strands while displacing the existing complementary strand, without requiring thermal denaturation, the property that makes isothermal amplification possible. Choose standard Bst DNA polymerase for DNA-template LAMP; choose engineered Bst variants (Bst 2.0, Bst 3.0, or lyo-ready formats) for RT-LAMP applications requiring combined reverse transcriptase activity, faster reaction speed, or room-temperature-stable formats. 
<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="6ukg4n" data-start="2051" data-end="2199">Strong strand-displacement DNA synthesis using Bst DNA polymerase variants, enabling rapid isothermal amplification without thermal cycling.</li>
<li data-section-id="7ycop5" data-start="2200" data-end="2330">High-efficiency Phi29 DNA polymerase systems supporting whole-genome amplification through processive strand displacement.</li>
<li data-section-id="18wphsf" data-start="2331" data-end="2463">Enzyme formats optimised for fast, constant-temperature nucleic acid amplification across LAMP and related isothermal methods.</li>
</ul>
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<h2 dir="ltr">How to Choose a LAMP Polymerase</h2>
 Strand-Displacement Activity Bst DNA polymerase's large fragment (Bst LF) provides the core strand-displacement activity that enables LAMP, operating at a temperature optimum near 65 &deg;C -- this activity allows continuous synthesis and primer annealing on single-stranded regions generated during amplification, without thermal cycling. Reverse Transcription Capability Engineered variants such as Bst 3.0 may offer enhanced reverse transcription capability, enabling RT-LAMP workflows by supporting RNA-to-cDNA conversion within the same isothermal reaction environment. Reaction Speed Optimised Bst formulations can support rapid amplification in LAMP reactions, with performance depending on primer design, template quality, and reaction conditions. Format: Liquid vs. Lyophilised Lyo-ready (lyophilised) formats support room-temperature shipping and storage, relevant for field-deployable or point-of-need applications where cold-chain logistics are impractical. Standard liquid formulations require frozen storage but may offer cost advantages for laboratory-based use. Pairing with Reverse Transcriptase For RT-LAMP using polymerases without intrinsic RT activity, pairing with a reverse transcriptase whose temperature optimum overlaps with the Bst polymerase's working temperature (commonly 60-65 &deg;C) helps maintain the isothermal format -- engineered high-thermostability reverse transcriptases are better suited to this than standard AMV or M-MLV enzymes with lower temperature optima. 
<h2 dir="ltr"> Specifications Context</h2>
 Bst DNA polymerase large fragment drives LAMP through strand-displacement activity at its temperature optimum near 65 &deg;C; because LAMP uses this single-temperature synthesis instead of thermal denaturation, the enzyme's strand-displacement capability -- rather than thermostability across a wide cycling range as with Taq -- is the key performance attribute. When combining AMV or M-MLV reverse transcriptases (with lower native temperature optima) with Bst polymerase for RT-LAMP, the temperature mismatch can require a two-temperature protocol, undermining the isothermal format; this has driven engineering efforts producing polymerases such as Bst 3.0 with intrinsic reverse transcriptase activity functioning at the Bst temperature optimum. Comparative testing has shown lyo-ready Bst DNA polymerase formulations can provide significantly higher RT-LAMP reaction speed than some other commercially available Bst polymerases when tested with the same RNA targets and primer sets. LAMP polymerases are typically stored at &minus;20 &deg;C (liquid formats) or at room temperature (lyo-ready formats per product specification); lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to find polymerases suited to your LAMP and isothermal amplification workflows.

Polymerases

MSDS - Ultra-Pure Phi29 DNA Polymerase

Phi29 DNA Polymerase

E014 by Applied Biological Materials is Ultra-Pure Phi29 DNA Polymerase, 100 µL (~200 reactions) with 5× Reaction Buffer (0.5 µg/µL BSA). Powerful strand-displacement + 3′→5′ proofreading. Isothermal RCA, MDA, WGA. Ultra-pure: TaqMan qPCR DNA contamination verified. Store at −20°C.

Format General

Storage Buffer

Caution

<h2 dir="ltr">What is a qPCR, Titer Kit?</h2>
 A qPCR titer kit provides the primers, probes, or dye-based detection chemistry, standards, and master mix components needed to quantify viral vector genome copy number by real-time PCR. For lentiviral preparations, this typically involves amplifying a conserved region of the viral genome (such as the LTR or a packaging signal sequence) and comparing the signal to a standard curve of known copy number to determine vector genomes per mL of the preparation. Use a qPCR titer kit for rapid, same-day genomic titer determination of in-house viral vector preparations, understanding that genomic titer (genome copies/mL) is related to, but distinct from, functional (infectious) titer measured by transduction assays.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Lentivirus &amp; Retrovirus Titer Kits: Advanced quantitative qPCR-based tools designed for rapid and accurate assessment of viral genome copy number in lentiviral and retroviral preparations, supporting consistent transduction workflow optimisation in mammalian cell culture systems.
</li>
<li dir="ltr" aria-level="1">
AAV Titer Kits: Optimised qPCR solutions for the quantification of Adeno-Associated Virus vectors, providing precise genome copy data required for vector dosing and therapeutic research studies.
</li>
</ul>
<h2 dir="ltr"> How to Choose a qPCR Titer Kit</h2>
 Viral Vector Type Compatibility qPCR titer kits are commonly available for lentivirus, with related kits for AAV and other viral vector types -- confirm the kit targets the appropriate conserved genomic region for your specific vector type and packaging system. Genomic vs. Functional Titer qPCR titer kits report genomic titer (vector genome copies per mL), which can differ from functional or infectious titer measured by transduction-based assays (such as a GFP or antibiotic-resistance endpoint assay) -- understanding this distinction is important when translating a qPCR titer result into an experimental MOI. Standard Curve and Quantification Range The kit's included standards define the quantification range over which results are reliable -- confirm your expected titer falls within this range, diluting samples as needed to bring concentrated preparations into range. Turnaround Time qPCR titer kits provide same-day results (the time required for a qPCR run plus data analysis), substantially faster than functional transduction-based titer assays, which require a transduction step followed by several days of cell culture and an endpoint readout. Compatibility with Existing qPCR Infrastructure Since qPCR titer kits use standard real-time PCR instrumentation, confirm that the kit's detection chemistry (dye-based or probe-based) and any reference dye requirements (such as ROX) are compatible with your existing real-time PCR instrument. 
<h2 dir="ltr"> Specifications Context</h2>
 qPCR-based viral titer determination amplifies a conserved genomic region of the vector -- for lentivirus, commonly within the LTR or packaging signal -- and compares the resulting signal against a standard curve of known copy number to calculate vector genomes per mL of the test preparation. Because genomic titer counts total vector particles containing the packaged genome regardless of whether they are capable of productive transduction, it typically yields a higher number than functional titer, which counts only particles that successfully transduce and express in target cells; researchers calculating MOI for an experiment should be clear about which titer type they are using, since the appropriate MOI range can differ depending on which titer figure is the basis for the calculation. qPCR titer kits are typically stored at &minus;20 &deg;C, with standards and master mix components included; lot-specific CoAs are available on request. 
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qPCR Titer Kits

ROX Machine Compatibility

qPCR AAV Titer Kit

G931 by Applied Biological Materials is a 100-reaction qPCR AAV titer kit. One-step lysis + qPCR with proprietary NTC-eliminating primers. Includes DNase I Reaction Mix for unencapsidated DNA removal. SYBR Green–comparable dye; ROX supplied separately. ISO 13485:2016 MDSAP certified. Store at −20°C; stable 2 years.

Note

qPCR Lentivirus Titer Kit

LV900 by Applied Biological Materials is a 100-reaction one-step RT-qPCR lentiviral titer kit. No RNA purification required — direct use on clarified supernatant. Proprietary primer design eliminates NTC amplification. SYBR Green–comparable dye; ROX supplied separately. ISO 13485:2016 MDSAP certified. Store at −20°C; stable 2 years.

qPCR Retrovirus Titer Kit

G949 by Applied Biological Materials is the qPCR Retrovirus Titer Kit — 100 reactions. One-step RT-qPCR directly from viral supernatant; no purification needed. BlasTaq™ MasterMix (SYBR/EvaGreen-comparable dye); proprietary primers — no NTC amplification. ISO 13485:2016 MDSAP Certified. ROX Reference Dye included. 2-year stability. Store at −20°C.

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
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<h2 data-start="432" data-end="459">What you will find:</h2>
&nbsp;
<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
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Miscellaneous Items

Miscellaneous items

Random Primer (6N)

Random Primer (6N) G139 — 10 µM random hexamers in ddH₂O, 500 µl. For reverse transcription cDNA synthesis from total RNA or mRNA. Recommended final concentration: 0.5–1 µM. Store at −20 °C up to 60 months; avoid freeze–thaw cycles.

Recommended Protocol

Shipping condition

Taq 2X PCR MasterMix

G888 Taq 2X PCR MasterMix by Applied Biological Materials is a cost-effective, ready-to-use 2X Taq PCR master mix (1.5 mM Mg2+; gel loading dye) for routine amplification producing 3&prime;-dA-tailed TA-cloning-compatible products. 400 rxn (10.0 ml); ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.