PCR Reagents & Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

DataSheet

MSDS

PCR Product Guide

All-In-One 5X RT MasterMix with gDNA Removal

G592 All-In-One 5X RT MasterMix with gDNA Removal by Applied Biological Materials is a complete single-tube RT master mix with integrated temperature-sensitive DNase for in-reaction gDNA elimination, OneScript&reg; Hot thermostable RT (RNase H-deficient, 60&ndash;72&deg;C), and RNaseOFF inhibitor. 100 rxn (400 µl); ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

Unit Quantity

Application

Storage Condition

Concentration

Shipping Condition

<h2 dir="ltr">What Is a Hot Start Taq Master Mix?</h2>
 Hot start 2x master mixes combine the convenience of the ready-to-use master mix format with the improved specificity of hot-start technology. These products are the standard reagent format for high-throughput genotyping, clinical diagnostic PCR, and multiplex reactions where non-specific amplification would compromise results. The right hot-start master mix supports cleaner amplification and streamlined setup. Select a formulation designed for reliable PCR performance.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Hot-start formulations from Azura, Accuris, BlasTaq, and Zymo Research for reduced non-specific amplification and primer-dimer formation.</li>
<li>Antibody-mediated hot-start activation in selected products, including ZymoTaq PreMix and Accuris Hot Start Taq Master Mix.</li>
<li>2X master mix and premix formats containing hot-start Taq polymerase, MgCl₂, dNTPs, and optimized PCR buffers.</li>
<li>Red-dye formulations for direct gel loading, including Azura 2X HS Taq Red Mix and Accuris Hot Start Taq Master Mix Red Dye.</li>
<li>Buffer systems optimized for endpoint PCR, multiplex PCR, and challenging template amplification.</li>
</ul>
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<h2 dir="ltr">How to Choose a Hot Start Taq Master Mix</h2>
 Activation Mechanism Format Antibody-mediated hot-start master mixes activate during the standard initial denaturation step; chemically modified formats may require a longer or dedicated activation incubation &mdash; confirm cycling protocol compatibility before adopting a new product. Component Pre-Optimisation A 2x hot-start Taq master mix is a pre-formulated, double-concentrated solution containing hot-start Taq DNA polymerase, dNTPs, MgCl2, and PCR buffer &mdash; the hot-start polymerase remains inactive at room temperature until the activation step is reached during cycling. Multiplex Compatibility For multiplex PCR (multiple primer pairs in a single reaction), hot-start master mixes substantially reduce primer-primer cross-reactivity compared to standard Taq master mixes, improving reliable detection of all intended targets. Diagnostic and Clinical Applications High-specificity master mixes are the standard reagent format for clinical diagnostic PCR, where false-positive bands from non-specific amplification could affect result interpretation. Throughput and Format 2x hot-start master mixes are designed for high-throughput genotyping pipelines, requiring only template and primer addition &mdash; minimising pipetting steps across large sample sets. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start 2x master mixes are set up like standard master mixes&mdash;combined with template and primers at the manufacturer-specified ratio to achieve a 1x working concentration&mdash;but the polymerase remains inactive until the activation step, typically during initial denaturation for antibody-mediated formats. This combination of convenience and specificity has made hot-start master mixes a widely used choice for high-throughput genotyping, multiplex PCR, and other applications requiring reliable target-specific amplification. Product availability reflects MBP's catalogue as of mid-2026. Labs validating a new hot-start master mix should evaluate performance using known positive and negative samples and document results by lot number to support quality assurance, audits, and troubleshooting. 
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Hot Start Taq Master Mix

Flyer

BlasTaq 2X PCR MasterMix

G895 BlasTaq&trade; 2X PCR MasterMix by Applied Biological Materials is a ready-to-use 2X PCR master mix with BlasTaq&trade; DNA Polymerase (6 kb/min; 40&ndash;70% faster than Taq), 1.5 mM Mg2+, and integrated gel loading dye. Produces 3&prime;-dA-tailed TA-cloning-compatible amplicons. 800 rxn (10.0 ml); ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

Primer-Probe

ROX Machine Compatibility

BlasTaq™ 2X qPCR MasterMix Product Testing Report

Blastaq™ 2X qPCR MasterMix Product Stability Report

BlasTaq 2X qPCR MasterMix

G891 BlasTaq&trade; 2X qPCR MasterMix by Applied Biological Materials is a SYBR Green-compatible 2X qPCR master mix with BlasTaq&trade; DNA Polymerase (up to 70% faster than Taq), intercalating dye, and ROX Reference Dye (50 µl, separate). 500 rxn (4 &times; 1.25 ml); also available as G892 (2,500 rxn). ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

G892 by Applied Biological Materials is the BlasTaq&trade; 2X qPCR MasterMix (2,500 rxn / 25.0 ml) with next-generation BlasTaq&trade; DNA Polymerase (up to 70% faster than wild-type Taq), SYBR Green&trade;/EvaGreen&trade;-compatible intercalating dye, and separate ROX Reference Dye (240 µl). 2-year shelf life at &minus;20°C. ISO 13485:2016 MDSAP Certified. For 500 rxn format, see G891.

Note

<h2 dir="ltr">What Is Hot Start Taq DNA Polymerase?</h2>
 Hot-start Taq DNA polymerase is the enzyme of choice for PCR applications where non-specific amplification or primer dimer formation has been problematic with standard Taq. By keeping the polymerase inactive until a high-temperature activation step, hot-start formulations prevent the room-temperature primer extension that drives non-specific products in standard Taq reactions. Choose a polymerase suited to your assay requirements.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1hsv5i3" data-start="233" data-end="391">Hot-start Taq DNA polymerases are designed to support multiplex PCR by reducing non-specific amplification and primer-primer interactions during reaction setup.</li>
<li data-section-id="bhgh5r" data-start="392" data-end="516">Formulations suitable for high-throughput PCR workflows requiring a stable room-temperature setup before thermal activation.</li>
<li data-section-id="1h2z623" data-start="517" data-end="640">Improved specificity for amplification of low-abundance targets compared with conventional non-hot-start Taq polymerases.</li>
<li data-section-id="1nifv8r" data-start="641" data-end="743">Cleaner amplicon generation for downstream applications such as cloning, sequencing, and genotyping.</li>
<li data-section-id="bof93a" data-start="744" data-end="892">Enzyme and buffer systems formulated to support amplification of challenging templates, including GC-rich regions and complex genomic DNA samples.</li>
</ul>
<h2 dir="ltr"> How to Choose Hot Start Taq DNA Polymerase</h2>
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Inactivation Mechanism Antibody-mediated formulations use a heat-sensitive antibody that reversibly binds and inhibits the enzyme until denatured during the initial high-temperature step. Chemically modified formulations use covalent modifications to active-site residues that are reversed by heat during cycling. Standard vs. Hot-Start Comparison Standard Taq DNA polymerase is a formulation of recombinant Taq that remains active at ambient temperatures, permitting non-specific primer extension during reaction setup &mdash; hot-start formulations are reversibly inactivated at ambient temperature specifically to prevent this. Buffer Compatibility As with standard Taq, optimised 10x PCR buffer formulations are designed to complement the specific hot-start enzyme's activation and activity profile. Activation Time Considerations Confirm whether your chosen hot-start formulation activates within a standard initial denaturation step or requires an extended/dedicated activation incubation, as this affects total cycling protocol time. Application Fit Hot-start Taq is recommended wherever specificity is a concern: multiplex PCR, low-template-abundance amplification, diagnostic PCR, and any workflow where primer dimer formation has been observed with standard Taq. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start Taq DNA polymerase is a recombinant Taq formulation that remains inactive during reaction setup, preventing the non-specific primer extension that standard Taq can permit before thermal cycling begins. This improved specificity is particularly valuable for multiplex PCR, low-abundance templates, and applications where non-specific products could affect result interpretation. Hot-start enzymes are typically stored at &minus;20 &deg;C and commonly supplied with matched PCR buffer. Because antibody-mediated and chemically modified formats can require different activation conditions, initial denaturation parameters should be verified when introducing a new enzyme. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote and identify the most suitable hot start Taq DNA polymerase for your workflow.

Hot Start Taq DNA Polymerase

BlasTaq DNA Polymerase

G894 BlasTaq&trade; DNA Polymerase by Applied Biological Materials is a next-generation Taq enzyme with 6 kb/min extension speed (40&ndash;70% faster than wild-type Taq), 5&prime;&rarr;3&prime; exonuclease activity, and 3&prime;-dA-tailed TA-cloning-compatible products. Supplied as 400 rxn (200 µl) with 5X buffer (1.5 mM Mg2+). ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

BlasTaq™ HotStart 2X PCR MasterMix Product Testing Report

BlasTaq™ HotStart 2X PCR MasterMix Product Stability Report

BlasTaq HotStart 2X PCR MasterMix

G598 BlasTaq&trade; HotStart 2X PCR MasterMix by Applied Biological Materials is a room-temperature-stable Hot Start 2X PCR master mix with antibody-inhibited BlasTaq&trade; polymerase (up to 70% faster than Taq), integrated gel loading dye, and 3&prime;-dA-tailed products. 800 rxn (10.0 ml); ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

BlasTaq HotStart DNA Polymerase

G595 by Applied Biological Materials is BlasTaq&trade; HotStart DNA Polymerase (400 rxn; 200 µl enzyme + 5X Buffer 1 with 1.5 mM Mg2+). Antibody-mediated HotStart eliminates non-specific amplification and primer-dimers at room temperature setup. Up to 70% faster than wild-type Taq; 3'-dA overhangs for TA cloning. ISO 13485:2016 MDSAP Certified. MasterMix: G598. Store at &minus;20°C.

BlasTaq Probe 2X qPCR MasterMix

G890 BlasTaq&trade; Probe 2X qPCR MasterMix by Applied Biological Materials is a TaqMan&trade; probe-based 2X qPCR master mix containing BlasTaq&trade; DNA Polymerase (6 kb/min; 40&ndash;70% faster than Taq), dNTPs, MgCl&sub2;, and proprietary buffer. Supplied as 500 rxn (4 &times; 1.25 ml) with ROX Reference Dye (50 µl). ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

BlasTaq™ Probe One-Step RT-qPCR

G596 BlasTaq&trade; Probe One-Step RT-qPCR by Applied Biological Materials combines reverse transcription and TaqMan&trade; probe-based qPCR in a single tube for direct RNA quantification. Kit includes BlasTaq&trade; Probe 2X qPCR MasterMix (1.25 ml), RT-qPCR Enzyme Mix (100 rxn/40 µl), ROX Dye (15 µl), and Nuclease-Free H&sub2;O. ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

<h2 dir="ltr">What Are LAMP Polymerases?</h2>
 The defining enzyme of loop-mediated isothermal amplification is Bst DNA polymerase, isolated from Geobacillus stearothermophilus (formerly Bacillus stearothermophilus). Its high strand-displacement activity allows it to synthesise new DNA strands while displacing the existing complementary strand, without requiring thermal denaturation, the property that makes isothermal amplification possible. Choose standard Bst DNA polymerase for DNA-template LAMP; choose engineered Bst variants (Bst 2.0, Bst 3.0, or lyo-ready formats) for RT-LAMP applications requiring combined reverse transcriptase activity, faster reaction speed, or room-temperature-stable formats. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="6ukg4n" data-start="2051" data-end="2199">Strong strand-displacement DNA synthesis using Bst DNA polymerase variants, enabling rapid isothermal amplification without thermal cycling.</li>
<li data-section-id="7ycop5" data-start="2200" data-end="2330">High-efficiency Phi29 DNA polymerase systems supporting whole-genome amplification through processive strand displacement.</li>
<li data-section-id="18wphsf" data-start="2331" data-end="2463">Enzyme formats optimised for fast, constant-temperature nucleic acid amplification across LAMP and related isothermal methods.</li>
</ul>
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<h2 dir="ltr">How to Choose a LAMP Polymerase</h2>
 Strand-Displacement Activity Bst DNA polymerase's large fragment (Bst LF) provides the core strand-displacement activity that enables LAMP, operating at a temperature optimum near 65 &deg;C -- this activity allows continuous synthesis and primer annealing on single-stranded regions generated during amplification, without thermal cycling. Reverse Transcription Capability Engineered variants such as Bst 3.0 may offer enhanced reverse transcription capability, enabling RT-LAMP workflows by supporting RNA-to-cDNA conversion within the same isothermal reaction environment. Reaction Speed Optimised Bst formulations can support rapid amplification in LAMP reactions, with performance depending on primer design, template quality, and reaction conditions. Format: Liquid vs. Lyophilised Lyo-ready (lyophilised) formats support room-temperature shipping and storage, relevant for field-deployable or point-of-need applications where cold-chain logistics are impractical. Standard liquid formulations require frozen storage but may offer cost advantages for laboratory-based use. Pairing with Reverse Transcriptase For RT-LAMP using polymerases without intrinsic RT activity, pairing with a reverse transcriptase whose temperature optimum overlaps with the Bst polymerase's working temperature (commonly 60-65 &deg;C) helps maintain the isothermal format -- engineered high-thermostability reverse transcriptases are better suited to this than standard AMV or M-MLV enzymes with lower temperature optima. 
<h2 dir="ltr"> Specifications Context</h2>
 Bst DNA polymerase large fragment drives LAMP through strand-displacement activity at its temperature optimum near 65 &deg;C; because LAMP uses this single-temperature synthesis instead of thermal denaturation, the enzyme's strand-displacement capability -- rather than thermostability across a wide cycling range as with Taq -- is the key performance attribute. When combining AMV or M-MLV reverse transcriptases (with lower native temperature optima) with Bst polymerase for RT-LAMP, the temperature mismatch can require a two-temperature protocol, undermining the isothermal format; this has driven engineering efforts producing polymerases such as Bst 3.0 with intrinsic reverse transcriptase activity functioning at the Bst temperature optimum. Comparative testing has shown lyo-ready Bst DNA polymerase formulations can provide significantly higher RT-LAMP reaction speed than some other commercially available Bst polymerases when tested with the same RNA targets and primer sets. LAMP polymerases are typically stored at &minus;20 &deg;C (liquid formats) or at room temperature (lyo-ready formats per product specification); lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to find polymerases suited to your LAMP and isothermal amplification workflows.

Polymerases

Bst DNA Polymerase

G880 by Applied Biological Materials is Bst DNA Polymerase Large Fragment from Geobacillus stearothermophilus. Strand-displacement + 5′→3′ synthesis; no exonuclease. Optimal at 60–65°C. For LAMP, SDA, WGA, and isothermal nucleic acid amplification. Store at −20°C.

<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
</ul>
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<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

dNTP Mix

dNTP Mix G129 (ABM) — 1.0 ml pre-mixed aqueous solution of dATP, dCTP, dGTP, and dTTP, each at 10 mM. &gt;99% HPLC purity, DNase/RNase-free, functionally tested in PCR and qPCR. Stable 2 years at −20 °C. For PCR, RT-PCR, cDNA synthesis, and LAMP/RDA/MDA.

Product Features

Caution

Shipping condition

<h2 dir="ltr">What is a dNTP Set?</h2>
 A dNTP Set is a complete set of individual, highly pure deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - each supplied as a separate 100 mM solution in water. Each nucleotide can be diluted and mixed with any of the other three to the desired final concentration or ratio, giving researchers full control over custom PCR, qPCR, or labelling mix preparation. Choose a dNTP Set when you need to prepare custom-concentration mixes, run multiple applications with different nucleotide requirements, or want individual stocks for non-equimolar formulations.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="color: #000000;" data-section-id="rmv9gj" data-start="173" data-end="310">Pre-mixed deoxynucleotide triphosphate (dNTP) sets for PCR and DNA synthesis applications, including PureStrand&trade; dNTP Set (Azura)</li>
<li style="color: #000000;" data-section-id="1u2l5t9" data-start="311" data-end="437">High-concentration nucleotide formulations such as Accuris 100mM dNTP Set for flexible reaction setup and optimization</li>
<li style="color: #000000;" data-section-id="kkyf1s" data-start="438" data-end="533">Standard dNTP set formats suitable for routine PCR workflows, including dNTP Set (G050)</li>
<li style="color: #000000;" data-section-id="1j5qt5c" data-start="534" data-end="653">Nucleotide triphosphate formulations for enzymatic and molecular biology applications, including NTP Set (G946)</li>
<li style="color: #000000;" data-section-id="1nhlqes" data-start="654" data-end="773">Ready-to-use balanced nucleotide sets supporting DNA polymerase activity in PCR and related amplification reactions</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a dNTP Set</h2>
 Custom Mix Preparation A dNTP Set's primary advantage is flexibility - preparing a working mix at any desired total concentration and ratio, including non-standard formulations for specific applications such as nucleotide analog substitution (e.g., dUTP in place of dTTP for anti-carryover-contamination strategies). Multiple Application Support Labs running PCR, qPCR, cDNA synthesis, and DNA labelling from the same stock benefit from a single dNTP Set, since each application may call for slightly different concentrations or ratios prepared from the same individual 100 mM tubes. Stock Concentration 100 mM is the standard individual stock concentration for dNTP Sets, providing substantial dilution headroom - a 100 mM stock can be diluted 1000-fold to reach a 0.1 mM working concentration, or combined at lower dilution ratios for higher-concentration premixes. Equimolar Mixing Discipline When preparing a standard equimolar mix from a dNTP Set, equal volumes of each 100 mM nucleotide are combined with water to the desired total concentration - for example, equal-volume mixing of all four 100 mM stocks yields a 25 mM-each premix at 100 mM total. Purity and Lot Testing PCR-grade dNTP Sets are typically tested at greater than 99% purity by HPLC, with concentration verified by optical density spectrometry and absence of human/E. coli DNA confirmed by qPCR - important documentation for labs requiring lot-specific quality records. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard equimolar working mix from a dNTP Set, combining equal volumes of each 100 mM individual stock yields a mix at 25 mM of each nucleotide (100 mM total); this can be further diluted to common working stocks such as 10 mM each (a 50x stock relative to the 0.2 mM PCR final concentration) or 2.5 mM each (a 10x stock). The performance of each lot is typically functionally tested in a PCR requiring all four nucleotides, with concentration verified by optical density spectrometry and purity confirmed by HPLC. dNTP Sets are stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing each individual tube thoroughly before use is recommended. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get reliable dNTP sets for your next successful experiment.

dNTP Set

dNTP Set G050 (ABM) — four separate 250 µl vials of dATP, dCTP, dGTP, and dTTP, each at 100 mM aqueous solution. &gt;99% purity (HPLC), DNase/RNase-free, functionally tested in PCR and qPCR. Stable 2 years at −20 °C. For PCR, RT-PCR, cDNA synthesis, and LAMP/RDA/MDA.