PCR Reagents & Kits

<h2 dir="ltr">What Is a Hot Start Taq Master Mix?</h2>
 Hot start 2x master mixes combine the convenience of the ready-to-use master mix format with the improved specificity of hot-start technology. These products are the standard reagent format for high-throughput genotyping, clinical diagnostic PCR, and multiplex reactions where non-specific amplification would compromise results. The right hot-start master mix supports cleaner amplification and streamlined setup. Select a formulation designed for reliable PCR performance.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Hot-start formulations from Azura, Accuris, BlasTaq, and Zymo Research for reduced non-specific amplification and primer-dimer formation.</li>
<li>Antibody-mediated hot-start activation in selected products, including ZymoTaq PreMix and Accuris Hot Start Taq Master Mix.</li>
<li>2X master mix and premix formats containing hot-start Taq polymerase, MgCl₂, dNTPs, and optimized PCR buffers.</li>
<li>Red-dye formulations for direct gel loading, including Azura 2X HS Taq Red Mix and Accuris Hot Start Taq Master Mix Red Dye.</li>
<li>Buffer systems optimized for endpoint PCR, multiplex PCR, and challenging template amplification.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a Hot Start Taq Master Mix</h2>
 Activation Mechanism Format Antibody-mediated hot-start master mixes activate during the standard initial denaturation step; chemically modified formats may require a longer or dedicated activation incubation &mdash; confirm cycling protocol compatibility before adopting a new product. Component Pre-Optimisation A 2x hot-start Taq master mix is a pre-formulated, double-concentrated solution containing hot-start Taq DNA polymerase, dNTPs, MgCl2, and PCR buffer &mdash; the hot-start polymerase remains inactive at room temperature until the activation step is reached during cycling. Multiplex Compatibility For multiplex PCR (multiple primer pairs in a single reaction), hot-start master mixes substantially reduce primer-primer cross-reactivity compared to standard Taq master mixes, improving reliable detection of all intended targets. Diagnostic and Clinical Applications High-specificity master mixes are the standard reagent format for clinical diagnostic PCR, where false-positive bands from non-specific amplification could affect result interpretation. Throughput and Format 2x hot-start master mixes are designed for high-throughput genotyping pipelines, requiring only template and primer addition &mdash; minimising pipetting steps across large sample sets. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start 2x master mixes are set up like standard master mixes&mdash;combined with template and primers at the manufacturer-specified ratio to achieve a 1x working concentration&mdash;but the polymerase remains inactive until the activation step, typically during initial denaturation for antibody-mediated formats. This combination of convenience and specificity has made hot-start master mixes a widely used choice for high-throughput genotyping, multiplex PCR, and other applications requiring reliable target-specific amplification. Product availability reflects MBP's catalogue as of mid-2026. Labs validating a new hot-start master mix should evaluate performance using known positive and negative samples and document results by lot number to support quality assurance, audits, and troubleshooting. 
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Hot Start Taq Master Mix

DataSheet

MSDS

PCR Product Guide

Kodaq 2X PCR MasterMix

G883 Kodaq 2X PCR MasterMix by Applied Biological Materials is a ready-to-use 2X master mix containing Kodaq DNA Polymerase with 3&prime;&rarr;5&prime; proofreading activity, dNTPs, optimized buffer, and gel loading dye. Delivers 800 reactions (10.0 ml); tolerates A/T-rich, G/C-rich, and inhibitor-containing templates.

Unit Quantity

Note

Shipping Condition

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

One-Step RT-PCR Kits

MegaFi One-Step RT-PCR Kit

G597 by Applied Biological Materials is the MegaFi&trade; Pro One-Step RT-PCR Kit (100 rxn) — single-tube RT + PCR with OneScript&reg; Hot RT (thermostable, 50–80°C cDNA synthesis), MegaFi&trade; Pro Fidelity Polymerase (&gt;2000X lower error than Taq), and RNaseOFF Inhibitor. 2X Buffer includes gel loading dye. ISO 13485:2016 MDSAP Certified. Store at &minus;20°C.

Storage Condition

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
</ul>
<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

High-Fidelity Master Mix

MegaFi™ Pro Fidelity 2X Mastermix

Flyer

MegaFi Pro Fidelity 2X PCR MasterMix

G887 MegaFi&trade; Pro Fidelity 2X PCR MasterMix by Applied Biological Materials is an ultra-high-fidelity 2X PCR master mix with &gt;2,000&times; lower error rate than Taq, Hot Start monoclonal antibody, blunt-end products, and integrated gel loading dye. 800 rxn (10.0 ml); ISO 13485:2016 MDSAP certified; stable 2 years at &minus;20&deg;C.

Concentration

<h2>What Are High-Fidelity DNA Polymerases?</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases transformed PCR from a tool for detection and amplification into a precise DNA engineering platform. By combining thermostability with 3' to 5' proofreading exonuclease activity, modern high-fidelity enzymes detect and remove misincorporated bases immediately after insertion during DNA synthesis. Polymerase fidelity can significantly affect downstream results, so choosing an enzyme that meets your accuracy and performance requirements is essential.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>High-fidelity DNA amplification with proofreading activity using Azura TruFi&trade; II DNA Polymerase.</li>
<li>Proofreading polymerase formulations with matched reaction buffers, including ExtremeTaq&trade; DNA Polymerase with 5x Reaction Buffer.</li>
<li>Accurate PCR amplification with Accuris High Fidelity DNA Polymerase for cloning, sequencing, and mutagenesis workflows.</li>
<li>High-fidelity enzyme systems featuring proofreading activity, including MegaFi Pro Fidelity DNA Polymerase.</li>
</ul>
&nbsp;
<h2>How to Choose a High-Fidelity DNA Polymerase</h2>
&nbsp;
Enzyme Family Selection
High-fidelity polymerases differ in processivity, extension speed, fidelity, and template compatibility. Selection should consider amplicon length, GC content, template complexity, and downstream application requirements.
Format: Enzyme vs. Master Mix
Individual high-fidelity enzymes with optimized HF buffers suit custom reaction optimization, particularly for difficult templates requiring buffer additive titration. In contrast, 2x master mixes streamline routine high-fidelity amplification for cloning and mutagenesis workflows.
Error Rate Requirements
For applications where amplicons will be cloned and sequence-verified downstream, the approximately 50- to 100-fold lower error rate of high-fidelity enzymes compared with standard Taq polymerase substantially reduces the frequency of sequence-confirmed clones carrying PCR-introduced mutations.
Extension Speed and Cycling Time
High-fidelity polymerases often extend faster than standard Taq per kilobase, which can shorten overall cycling time for long amplicons. However, optimal cycling parameters are enzyme-specific and should follow the manufacturer's recommendations.
Cloning Method Compatibility
Some high-fidelity polymerases generate blunt-ended PCR products suited to Gibson Assembly or blunt-end cloning strategies, while others may require additional steps for TA cloning. Confirm compatibility with your intended downstream cloning method before selection.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The development of high-fidelity thermostable DNA polymerases&mdash;combining thermostability with 3' to 5' proofreading exonuclease activity&mdash;transformed PCR from a detection and amplification tool into a precise DNA engineering platform, enabling reliable cloning, site-directed mutagenesis, and sequencing-grade amplification. The proofreading mechanism corrects misincorporated bases immediately after insertion, reducing error rates to approximately 10&minus;6 per nucleotide. Selection between high-fidelity polymerases often depends on template characteristics such as GC content, amplicon length, secondary structure, and downstream cloning requirements. High-fidelity enzymes are typically stored at &minus;20 &deg;C with matched HF buffer systems, and lot-specific Certificates of Analysis (CoAs) are available upon request. For sequencing-bound amplicons, confirm that the selected polymerase's documented error rate meets the level of sequence accuracy required, as even a 50- to 100-fold improvement over standard Taq may not be sufficient for applications demanding extremely low mutation rates across very long amplicons. Product availability reflects MBP's catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP </a>today to find high-fidelity DNA polymerase solutions for streamlining your laboratory workflows.

High-Fidelity DNA Polymerase

MegaFi Pro Fidelity DNA Polymerase

G886 MegaFi&trade; Pro Fidelity DNA Polymerase by Applied Biological Materials delivers an error rate &gt;2,000&times; lower than Taq polymerase via a Hot Start monoclonal antibody inhibitor mechanism, producing blunt-end PCR products for NGS library prep, cloning, CRISPR edit detection, and mutagenesis. ISO 13485:2016 MDSAP certified. Supplied as 400 rxn; stable 2 years at &minus;20&deg;C.

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

miRNA All-In-One cDNA Synthesis Kit

G898 miRNA All-In-One cDNA Synthesis Kit by Applied Biological Materials provides a 20-reaction, single-tube poly(A) tailing + reverse transcription workflow for miRNA first-strand cDNA synthesis using Poly(A) Polymerase, OneScript&reg; Plus RT, and a modified Oligo d(T) Adapter for universal miRNA coverage. Compatible with BlasTaq&trade; qPCR (G891). Store at &minus;20&deg;C; stable 2 years.

Mycoplasma PCR Detection Kit

Mycoplasma PCR Detection & Elimination Kit

Mycoplasma PCR Detection &amp; Elimination Kit (G238-G398) by Applied Biological Materials combines the Mycoplasma PCR Detection Kit (G238, 200+ strains, &lt;2 hr, no DNA isolation) with MycoAway™ 1000X Mycoplasma Cocktail (G398, 70+ species eliminated in 2–3 weeks). ISO 13485:2016 MDSAP certified. Shipped on ice pack.

G398-MSDS

Mycoplasma Pro PCR Detection Kit Competitor Comparison

Mycoplasma Pro PCR Detection & Elimination Kit

Mycoplasma Pro PCR Detection &amp; Elimination Kit (G239-G398) by Applied Biological Materials combines the upgraded G239 Pro kit (300+ strains, internal PCR control, A. laidlawii coverage, &lt;2 hr) with MycoAway™ G398 (70+ species eliminated in 2–3 weeks). ISO 13485:2016 MDSAP certified. Shipped on ice pack.

List of Detectable Species/Strains (Cat. G239)

Mycoplasma Pro PCR Detection Kit

Mycoplasma Pro PCR Detection Kit (G239) by Applied Biological Materials is a 100-reaction PCR kit detecting 300+ Mycoplasma and Acholeplasma strains (including A. laidlawii) in under 2 hours with no DNA isolation. Includes internal PCR control, BlasTaq™ MasterMix, Pro Primer Mix, and positive control. ISO 13485:2016 MDSAP certified. Store at –20°C; 2-year stability.

<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
</ul>
&nbsp;
<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

NTP Mix

G945 NTP Mix by ABM — pre-mixed ATP, CTP, GTP, UTP (25 mM each) in 1.0 ml; &gt;99% HPLC purity; IVT-tested; stable 2 years at −20°C; for SP6, T3, T7 IVT reactions. Simplifies NTP addition vs. individual NTP Set (G946). Shipped on Ice Pack.

Application

Product Features

Caution

Shipping condition

<h2 dir="ltr">What is a dNTP Set?</h2>
 A dNTP Set is a complete set of individual, highly pure deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - each supplied as a separate 100 mM solution in water. Each nucleotide can be diluted and mixed with any of the other three to the desired final concentration or ratio, giving researchers full control over custom PCR, qPCR, or labelling mix preparation. Choose a dNTP Set when you need to prepare custom-concentration mixes, run multiple applications with different nucleotide requirements, or want individual stocks for non-equimolar formulations.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="color: #000000;" data-section-id="rmv9gj" data-start="173" data-end="310">Pre-mixed deoxynucleotide triphosphate (dNTP) sets for PCR and DNA synthesis applications, including PureStrand&trade; dNTP Set (Azura)</li>
<li style="color: #000000;" data-section-id="1u2l5t9" data-start="311" data-end="437">High-concentration nucleotide formulations such as Accuris 100mM dNTP Set for flexible reaction setup and optimization</li>
<li style="color: #000000;" data-section-id="kkyf1s" data-start="438" data-end="533">Standard dNTP set formats suitable for routine PCR workflows, including dNTP Set (G050)</li>
<li style="color: #000000;" data-section-id="1j5qt5c" data-start="534" data-end="653">Nucleotide triphosphate formulations for enzymatic and molecular biology applications, including NTP Set (G946)</li>
<li style="color: #000000;" data-section-id="1nhlqes" data-start="654" data-end="773">Ready-to-use balanced nucleotide sets supporting DNA polymerase activity in PCR and related amplification reactions</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a dNTP Set</h2>
 Custom Mix Preparation A dNTP Set's primary advantage is flexibility - preparing a working mix at any desired total concentration and ratio, including non-standard formulations for specific applications such as nucleotide analog substitution (e.g., dUTP in place of dTTP for anti-carryover-contamination strategies). Multiple Application Support Labs running PCR, qPCR, cDNA synthesis, and DNA labelling from the same stock benefit from a single dNTP Set, since each application may call for slightly different concentrations or ratios prepared from the same individual 100 mM tubes. Stock Concentration 100 mM is the standard individual stock concentration for dNTP Sets, providing substantial dilution headroom - a 100 mM stock can be diluted 1000-fold to reach a 0.1 mM working concentration, or combined at lower dilution ratios for higher-concentration premixes. Equimolar Mixing Discipline When preparing a standard equimolar mix from a dNTP Set, equal volumes of each 100 mM nucleotide are combined with water to the desired total concentration - for example, equal-volume mixing of all four 100 mM stocks yields a 25 mM-each premix at 100 mM total. Purity and Lot Testing PCR-grade dNTP Sets are typically tested at greater than 99% purity by HPLC, with concentration verified by optical density spectrometry and absence of human/E. coli DNA confirmed by qPCR - important documentation for labs requiring lot-specific quality records. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard equimolar working mix from a dNTP Set, combining equal volumes of each 100 mM individual stock yields a mix at 25 mM of each nucleotide (100 mM total); this can be further diluted to common working stocks such as 10 mM each (a 50x stock relative to the 0.2 mM PCR final concentration) or 2.5 mM each (a 10x stock). The performance of each lot is typically functionally tested in a PCR requiring all four nucleotides, with concentration verified by optical density spectrometry and purity confirmed by HPLC. dNTP Sets are stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing each individual tube thoroughly before use is recommended. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get reliable dNTP sets for your next successful experiment.

dNTP Set

NTP Set

G946 NTP Set by ABM — 4×250 µl of ATP, CTP, GTP, and UTP (100 mM each); &gt;99% HPLC purity; functionally tested; stable 2 years at −20°C; for in vitro transcription with SP6, T3, and T7 RNA polymerases. Shipped on Ice Pack.

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
&nbsp;
<h2 data-start="432" data-end="459">What you will find:</h2>
&nbsp;
<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
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Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
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Miscellaneous Items

Miscellaneous items

Oligo(dT)18 Primer

Oligo(dT)18 Primer G140 — 10 µM, 500 µl in ddH₂O. Universal poly-A mRNA primer for reverse transcription cDNA synthesis at 0.5–1 µM final concentration. For RT-PCR, RT-qPCR, and cDNA library construction from poly-A mRNA. Store −20 °C, avoid freeze–thaw; stable up to 60 months.

Recommended Protocol

Product Literature

Product Stability Report

OneScript Hot 5X MasterMix

G590 OneScript&reg; Hot 5X RT MasterMix by Applied Biological Materials is a complete, ready-to-use 5X reverse transcription master mix containing OneScript&reg; Hot thermostable RT (RNase H-deficient, active at 60&ndash;72&deg;C), RNaseOFF inhibitor, dNTPs, and mixed primers for high-yield cDNA synthesis from challenging RNA. ISO 13485:2016 MDSAP certified; 100 rxn (400 µl); store at &minus;20&deg;C.