Cells, Tissue & Blood DNA/RNA Co-Extraction Kits
Cells/tissue/blood DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA from mammalian cell pellets, solid tissue biopsies, and whole blood in a single workflow that delivers separate fractions in under 15 minutes. Column-switching architecture physically separates gDNA from RNA before column binding—no sample splitting required.
MBP carries Zymo Research Quick-DNA/RNA Miniprep Plus, Microprep Plus, and MagBead kits for registered vendors, including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. Request a quote for DNA/RNA co-extraction kits designed for mammalian cells, tissue biopsies, and blood samples requiring matched genomic and transcriptomic analysis by contacting customerservice@mbpinc.net.
Cells/Tissue/Blood
Showing 1 to 5 of 5 results
What are Cells/Tissue/Blood DNA/RNA Co-Extraction Kits?
Cells/tissue/blood DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA from the same mammalian cell pellet, solid tissue biopsy, or blood sample in a single lysis and purification workflow that delivers separate DNA and RNA fractions without splitting the input. The approach conserves sample and eliminates the spatial heterogeneity introduced when a tissue biopsy is cut into two portions for separate DNA and RNA extractions, a significant limitation for samples with variable cellularity (tumor biopsies, mixed-cell-type tissues). Choose a cells/tissue/blood co-extraction kit when paired genomic sequencing and transcriptome data are required from the same physical sample, when sample volume is limited, or when downstream analyses demand that DNA and RNA originate from the same exact cellular population.
What you will find:
- Spin-column: Efficient silica-membrane kits including Quick-DNA/RNA™ Miniprep (D7001), Microprep Plus (D7005), and specialized Blood RNA Mini (R6411-00) tools for rapid, manual purification workflows.
- Mag Beads: Automated, high-throughput separation systems utilizing the Quick-DNA/RNA™ MagBead Kit (R2130) for high-yield, magnetic-based nucleic acid co-extraction.
How to Choose a Cells/Tissue/Blood DNA/RNA Co-Extraction Kit
Input Format: Cells vs. Tissue vs. Blood
For cell culture pellets (up to 5x10^6 cells): the Zymo Quick-DNA/RNA Miniprep or Miniprep Plus Kit provides the broadest compatibility. For solid tissue biopsies (up to 30 mg): the Plus kit (with DNA/RNA Shield and Proteinase K) handles tissue disruption without additional grinding. For whole blood (up to 50 ul): the Miniprep Plus kit is validated; for larger blood volumes requiring higher RNA mass, consider a dedicated blood RNA system for the RNA fraction. For very small inputs (needle biopsy cores, 500-5,000 cells): use the Microprep Plus Kit with 6-25 ul elution volume.
Spin-Column vs. Magnetic Bead Format
Spin-column co-extraction kits (Quick-DNA/RNA Miniprep, QIAGEN AllPrep) handle 1-24 samples per run and require only a microcentrifuge. The Quick-DNA/RNA MagBead Kit processes 96 samples per run on compatible automation platforms including Tecan, Hamilton, and Beckman Biomek. For clinical sample processing programs, gene therapy tissue panels, or large AAV biodistribution studies (10+ tissues x multiple animals), the magnetic bead format provides the throughput that prevents bottlenecks.
DNase I Treatment for the RNA Fraction
Physical gDNA depletion via the IIICG/IICR column-switching architecture removes most genomic DNA from the RNA fraction. For RNA-seq applications where any gDNA mapping artefacts are unacceptable, add the optional on-column DNase I treatment step using the included DNase I enzyme in Plus kits. Validate gDNA removal with a no-reverse-transcriptase control in RT-qPCR before proceeding to RNA-seq library preparation.
Small RNA Retention
If downstream applications include miRNA quantification, miRNA-seq, or small RNA sequencing alongside RNA-seq, confirm the kit retains RNAs >=17 nt. Zymo Quick-DNA/RNA kits retain the full small RNA spectrum on the IC column. QIAGEN AllPrep DNA/RNA kits under standard protocol retain RNA >=200 nt; a modified protocol in the AllPrep Micro Kit handbook enables small RNA retention from cell inputs.
FFPE Tissue
For archived FFPE tissue where both DNA mutation and RNA expression data are needed from the same section (paired cancer sequencing and transcriptomics), use the Zymo Quick-DNA/RNA FFPE Miniprep Kit, which includes deparaffinization solution, Proteinase K, and heat-mediated crosslink reversal for recovery of crosslinked DNA and fragmented RNA from paraffin-embedded material.
Specifications Context
Quality benchmarks for cells/tissue/blood co-extractions: DNA A260/A280 >= 1.8, fragment size >= 20 kb for whole-exome sequencing (>= 5 kb minimum for most NGS workflows); RNA A260/A280 >= 1.8, RIN >= 7 from fresh tissue (RIN 3-6 from FFPE). The Zymo Quick-DNA/RNA Miniprep Plus Kit delivers DNA and total RNA (including small RNAs >=17 nt) from any biological sample in less than 15 minutes, with both analytes ready for next-generation sequencing, RT-qPCR, and microarray without further cleanup. DNA/RNA co-extraction from the same tissue input is standard practice in cancer multi-omics research and gene therapy biodistribution studies - a 2024 PMC-published study validated automated co-extraction across 10 tissue types for simultaneous AAV vector copy number (DNA, qPCR) and transgene expression analysis (RNA, RT-qPCR), achieving a 100% sample pass rate for both analytes.
For kit selection guidance and pricing on the Zymo Quick-DNA/RNA product line for cells, tissue, and blood applications, contact the MBP team.
FAQ
