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Laboratory Aspirators for Cell Culture Media Removal and Supernatant Aspiration

 

Laboratory aspirators use an oil-free vacuum pump (−30 to −750 mbar, adjustable) to remove spent cell culture media, supernatants, and wash buffers from culture vessels, microplates, and centrifuge tubes through an autoclavable POM handpiece with standard 200–300 µL tip or Pasteur pipette adapter. A 2-liter collection bottle with overflow protection valve and a replaceable hydrophobic in-line filter protect the pump. Designed for biosafety cabinet use. MBP is a registered vendor to Howard Hughes Medical Institute, MD Anderson Cancer Center, and Vanderbilt University.

Contact customerservice@mbpinc.net to compare laboratory aspirator systems and request pricing or availability information.

Laboratory Aspirator

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Aspire™ Laboratory Aspirator
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USD1,510.09 - USD1,510.09
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USD1,078.63 - USD1,078.63
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Extra tubing set, 1 meter
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USD70.75
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USD50.54
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Graduated, PC collection bottle and lid (included)
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USD228.59
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USD163.28
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Handheld vacuum controller with single channel adapters
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USD426.22
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USD304.44
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Hydrophobic filter, pk/1 ( 1 each included)
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USD72.00
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USD51.43
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Option quick disconnect kit, includes 2 male/female attachments & silicone tubing
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USD210.57
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USD150.41
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Optional eight channel adapter, fine tip stainless steel
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USD208.97
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USD149.26
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Optional eight channel adapter, for use with 200ul pipette tips
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USD342.95
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USD244.97
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Optional single channel adapter, extra long (80mm) stainless steel
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USD77.11
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USD55.08
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Silicone O-ring for Aspire PC Collection Bottle
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USD70.75
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USD50.54
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Tabletop Vacuum Aspirator System
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USD743.01
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USD530.72
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What Are Laboratory Aspirators?

 

Laboratory aspirators are compact vacuum systems designed to remove liquids from cell culture vessels, microplates, centrifuge tubes, and other laboratory containers without manual pipetting. These systems typically consist of an oil-free vacuum pump, adjustable pressure controls, a collection bottle, overflow protection mechanisms, replaceable filters, tubing, and handheld aspiration tools. Laboratory aspirators are widely used for cell culture media removal, supernatant aspiration, and washing procedures in applications such as ELISA, immunoassays, and general laboratory workflows.

 

What you will find:

 

  • Laboratory Aspirator: Innovative systems like tabletop vacuum models and the Aspire™ model designed for routine waste handling, liquid removal, and cleanup of benchtop samples.
  • Laboratory Aspirator Accessories: Adapters (single and multi-channel), silicone tubing, hydrophobic filters, handheld controllers, and collection bottles for suction setup optimization.

 

How to Choose a Laboratory Aspirator

 

Vacuum Pressure Range and Adjustability
Choose a system with adjustable vacuum settings that match your applications. Lower vacuum levels are suitable for delicate cell culture work, while higher settings support rapid liquid removal from tubes, flasks, and larger vessels.

Collection Bottle Capacity
Collection bottle size influences how frequently waste must be emptied during operation. Standard capacities are suitable for most workflows, while larger bottles support high-throughput or extended use.

Overflow Protection Features
Reliable overflow protection prevents liquid from reaching the vacuum pump and ensures safe handling of biological materials.

Handpiece and Tip Compatibility
Ensure the handpiece supports pipette tips, Pasteur pipettes, and required adapters for your workflow.

Noise Levels and Biosafety Cabinet Compatibility
Compact, quiet systems are suitable for use in or near biosafety cabinets without disrupting airflow or workspace access.

Autoclavable Components
Check which parts are autoclavable to simplify sterilization and maintain laboratory hygiene standards.

 

Specifications Context

 

Modern laboratory aspirators typically include oil-free pumps, adjustable vacuum controls, overflow protection systems, and replaceable hydrophobic filters for safe and efficient liquid removal.

Proper maintenance, including filter replacement and tubing inspection, helps maintain performance and extends equipment lifespan. Laboratory aspirators remain essential tools in research, clinical, and biotechnology laboratories.

Your hunt for a laboratory aspirator is over. Reach out to the expert team at Molecular Biology Products Inc. (MBP) today for a personalized quote.

FAQ

A laboratory aspirator is a compact benchtop vacuum system used to remove liquids from laboratory vessels—cell culture flasks, multi-well plates, centrifuge tubes, and Petri dishes—by suction rather than manual pipetting. It consists of a quiet, oil-free vacuum pump, an adjustable vacuum pressure control (−30 to −750 mbar), a 2-liter collection bottle with overflow protection, a replaceable hydrophobic in-line filter, silicone tubing, and an autoclavable handheld handpiece that holds 200–300 µL pipette tips or Pasteur pipettes. Primary applications are removal of spent cell culture media, supernatants after centrifugation, and wash buffers from immuno-assay plates.
Laboratory aspirators provide fully adjustable vacuum from −30 to −750 mbar. For gentle aspiration of media from cell monolayers without detaching adherent cells, set vacuum to −50 to −150 mbar and aspirate at the edge of the well, angling the flask to pool media away from the cell layer. For rapid removal of supernatant from centrifuge tubes, −300 to −500 mbar provides faster aspiration without sacrificing control. For emptying large culture flasks quickly, maximum vacuum (up to −750 mbar) is appropriate. Always verify the pressure gauge reading before starting a cell culture aspiration session.
The overflow protection valve is a float-actuated safety valve built into the collection bottle lid that closes the vacuum line automatically when the liquid level reaches the bottle's maximum fill capacity. Without this valve, a full collection bottle would allow liquid to be drawn through the tubing into the vacuum pump, damaging the pump motor and potentially spraying biohazardous waste into the pump and surrounding area. The overflow protection valve is the primary safety feature of laboratory aspirator systems; inspect it for proper function before each use and replace the collection bottle if the valve mechanism is compromised.
The in-line hydrophobic/antibacterial filter is a disposable filter cartridge installed between the collection bottle and the vacuum pump in the aspirator tubing circuit. It captures aerosols, moisture, and biological contaminants before they reach the pump, protecting the pump motor from liquid damage and preventing release of biohazardous aerosols into the lab environment. Replace the in-line filter when it becomes visibly wet, darkened, or after every bottle emptying session involving biohazardous liquid—the filter's hydrophobic membrane loses effectiveness once saturated.
Yes—the handheld vacuum controller and adapter inserts on the Accuris Aspire, Benchmark Scientific, and equivalent laboratory aspirator models are made of POM (Polyoxymethylene), a high-stiffness engineering polymer that withstands autoclave sterilization at 121°C for 20 minutes. This allows the handpiece to be sterilized between cell culture experiments or between working with different cell lines, reducing cross-contamination risk. Confirm the autoclavability of each component in the OEM documentation before sterilizing—silicone tubing is also autoclavable, but polycarbonate collection bottles should be inspected for crazing or stress whitening before autoclaving.
The standard laboratory aspirator handpiece (Accuris Aspire/Benchmark V0020 design) accepts standard 200–300 µL disposable pipette tips via one adapter cone, and standard Pasteur pipettes and serological pipettes via the main opening. A second accessory adapter cone provides a thin stainless-steel capillary tip (~40 mm) for fine-control aspiration in narrow wells, small tubes, or locations where a full pipette tip would contact the cell layer. The 200–300 µL tip allows precise placement at the edge of microplate wells; the serological pipette adapter allows rapid clearing of larger vessels.
Laboratory aspirators are specifically designed for biosafety cabinet (BSC) use during cell culture work. Their compact footprint (typically 240 × 200 × 340 mm), quiet pump (≤60 dBA), and flexible handheld controller make them compatible with standard Class II BSC work zones. The collection bottle should be positioned within the BSC or immediately adjacent; the vacuum tubing passes through or under the BSC sash. The in-line hydrophobic filter must be functional whenever biohazardous material is aspirated inside the BSC, to prevent aerosol contamination of the pump and lab environment.
House (centralized building) vacuum systems provide vacuum through a fixed wall outlet, requiring the lab to connect a collection bottle and filter between the wall outlet and the aspiration handpiece. Laboratory aspirators include a self-contained oil-free vacuum pump, collection bottle, filter, and handpiece in one benchtop unit—no connection to building vacuum infrastructure required. Benchtop aspirators offer adjustable vacuum pressure control and a pressure gauge, allowing the operator to set gentle or aggressive suction for different applications; house vacuum pressure is typically fixed and unregulated at the point of use.
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