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Ultraviolet Chambers for DNA & Surface Decontamination

 

Ultraviolet chambers use high-intensity UV-C lamps at 254 nm to decontaminate exposed instrument surfaces, pipettes, and molds in enclosed stainless-steel boxes, eliminating DNA and inactivating bacteria and viruses without heat or chemicals. They are standard equipment in PCR labs, forensics, molecular diagnostics, and clinical sample preparation to prevent carry-over contamination. MBP stocks UV chambers and accessories.

Contact customerservice@mbpinc.net to compare UV chamber models and request pricing or a quote.

Ultraviolet Chamber

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Optional extra shelf, UV Transparent
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USD249.52
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USD178.23
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Optional extra shelf, stainless steel
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USD93.31
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USD66.65
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UVC Indicator Cards, pack of 25
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USD164.48
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USD117.48
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UVC Indicator Stickers, pack of 40
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USD150.02
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USD107.16
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What Are Ultraviolet Chambers?

 

Laboratory ultraviolet (UV) chambers are enclosed stainless-steel benchtop boxes equipped with high-intensity UV-C lamps operating at 254 nm, used to decontaminate exposed surfaces of instruments, pipettes, molds, and small tools that cannot be autoclaved or chemically disinfected. UV-C irradiation at 254 nm disrupts microbial DNA by forming pyrimidine dimers, preventing replication and rendering exposed microorganisms non-viable. Stainless-steel interior walls reflect UV radiation from all angles, minimizing shadow zones on items placed inside. UV chambers are standard equipment in forensic, PCR, molecular diagnostics, and clinical sample processing labs where DNA carry-over contamination between samples is a critical concern.

 

What you will find:

 

  • Stainless steel extra shelves: for expanded sample capacity
  • UV transparent shelves: for optimized UV exposure
  • UVC indicator stickers (pack of 40): for exposure verification
  • UVC indicator cards (pack of 25): for validation of sterilization cycles

 

How to Choose a UV Chamber

 

Chamber Volume and Work Area

Benchtop UV chambers range from compact units with an internal volume of approximately 15–20 liters (suitable for individual pipettes and small tools) to larger models accommodating full instrument sets or multi-item loads. Air Science UV-Box models, for example, have an internal height of 11.25 inches (286 mm), accommodating most standard lab instruments.

Lamp Configuration and Dose Delivery

UV-C efficacy is a function of lamp intensity (µW/cm²), the number and placement of lamps, and exposure time. Optimally positioned lamp arrays eliminate shadow zones; some models specify D99 dosages—the intensity and duration required for a 2-log (99%) reduction in specific pathogens. For critical applications such as forensic DNA work, request dose data for your target organisms from the manufacturer.

Safety Interlocks

Specify a model with a door interlock preventing lamp activation until the door is fully closed, a UV-absorbing transparent window for safe visual monitoring, and a keyed lock for controlled access. These features are standard on most current laboratory UV chambers.

Timer and Programmability

A built-in timer allowing user-defined exposure cycles (typically 15–30 minutes) is standard. Microprocessor-controlled models with programmable cycle libraries and a color viewscreen allow documentation of each decontamination event for GLP/GMP records.

Accessories

Interior shelves and hanging rods allow items to be positioned for maximum UV exposure on all surfaces. Verify accessory compatibility (shelving dimensions, hanging rod diameter) with your specific model before ordering separately. See the Ultraviolet Chamber Accessories sub-category for available options.

 

Specifications Context

 

UV-C wavelength 254 nm is the germicidal peak for DNA disruption; chambers operating outside the 250–265 nm range are less effective against target organisms. Lamp output degrades over time—replace 254 nm UV-C lamps per manufacturer schedule (typically annually) to maintain decontamination efficacy. UV-C light does not penetrate opaque materials, so all surfaces to be decontaminated must have a direct line of sight to the lamps; repositioning items partway through the cycle improves coverage. As of 2026, research published in Infection Control & Hospital Epidemiology confirms UV-C irradiation at 254 nm achieves greater than 99.999% inactivation of coronavirus analogs under defined dose conditions.

Contact now with the helpful team of Molecular Biology Products Inc. (MBP) to equip your lab with the right ultraviolet chamber.

FAQ

A laboratory UV chamber uses high-intensity shortwave UV-C light at 254 nm to destroy exposed surface DNA and inactivate bacteria and viruses on instruments, pipettes, molds, and small tools placed inside the enclosed stainless-steel box. UV-C irradiation at 254 nm disrupts the DNA of microorganisms, preventing replication and rendering them non-viable without chemical disinfectants or heat.
Laboratory UV decontamination chambers operate at 254 nm, in the UV-C band (200–280 nm), which is the wavelength range most effective for disrupting microbial DNA. Some forensic and clinical UV chambers specify dose-delivery data in µW·s/cm² (microwatt-seconds per square centimeter) for specific pathogens—consult the product documentation for exact dose and exposure time recommendations for your application.
Benchtop UV chambers decontaminate exposed surfaces of items placed inside the enclosed box; they do not sterilize the surrounding lab room, liquids, or bulk materials. UV-C light cannot penetrate opaque surfaces or liquids, so it is a surface-treatment step only. For room-level air disinfection, room-mounted UV germicidal irradiation (UVGI) fixtures are used separately.
Standard safety features on laboratory UV chambers include a UV-absorbing transparent door window (allowing visual monitoring without direct UV exposure), a door interlock that prevents lamp activation until the door is securely closed, a keyed door lock to prevent unauthorized operation, and a programmable timer for controlled exposure cycles. These features protect lab workers from UV-C radiation, which causes corneal damage and skin burns with direct exposure.
Typical exposure times for UV-C decontamination chambers range from 15 to 30 minutes for standard instrument surface decontamination, though exact times depend on lamp intensity, chamber geometry, and the target organism. Items must have all surfaces directly exposed to the UV lamps—UV-C does not penetrate around corners or beneath overlapping surfaces. Repositioning items halfway through the cycle improves coverage.
Standard accessories for laboratory UV chambers include interior shelves (to support flat or larger items), hanging rods (to suspend smaller tools for full 360-degree exposure), replacement UV-C lamps (at 254 nm, replaced per manufacturer schedule, typically annually), and turntables for rotating items during irradiation. MBP stocks UV chamber accessories under the Ultraviolet Chamber Accessories sub-category.
A UV chamber and an autoclave serve different decontamination purposes and cannot substitute for each other. UV chambers decontaminate exposed surfaces of small, heat-sensitive instruments that cannot tolerate autoclaving (e.g., PCR pipettes, molds, lab tools). Autoclaves sterilize to SAL 10⁻⁶ via moist heat at 121°C and are required for media, glassware, and biohazardous waste. Labs typically use both.
UV decontamination chambers are standard in forensic labs (to prevent DNA carry-over between evidence samples), PCR and molecular diagnostics labs (to eliminate amplicon contamination on pipettes and surfaces), clinical microbiology labs (to surface-decontaminate shared instruments), and pharmaceutical QC labs. They are also used in veterinary, dental, and pharmaceutical compounding settings for tool decontamination between procedures.
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