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250 mL Conical Centrifuge Tubes for Large-Scale Cell Processing and Bioprocessing

 

250 mL centrifuge tubes are polypropylene conical-bottom vessels measuring 60 mm × 160 mm, rated to 6,000–7,500 × g in a supported rotor with polyetherimide cushions, used for cell harvesting, viral concentration, and large-scale precipitation at process scale. MBP supplies sterile 250 mL conical tubes with HDPE plug-seal caps, certified RNase-free, DNase-free, and pyrogen-free, in cases of 70 tubes, shipping to US, Canadian, and international labs.

Need help selecting the right 250 mL centrifuge tubes for your application? — Contact our team at customerservice@mbpinc.net for product recommendations, bulk pricing, and purchasing assistance.

250ml Centrifuge Tubes

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What Are 250 mL Centrifuge Tubes?

 

250 mL centrifuge tubes are polypropylene conical-bottom vessels with approximate dimensions of 60 mm outer diameter and 160 mm height. They are rated for centrifugation speeds of approximately 6,000–7,500 × g when used in a compatible swing-bucket rotor with proper support cushions. These tubes fill the volume gap between 50 mL conical tubes and 500 mL large-volume tubes, making them suitable for mid- to large-scale processing workflows.

They are commonly used for cell culture harvests in the range of 150–240 mL per tube, viral concentration at bench-to-pilot scale, density-gradient separations, and preparative protein precipitation steps where multiple 50 mL tubes would otherwise be required for a single batch. The polypropylene tube body and HDPE plug-seal cap provide chemical resistance and temperature stability from −80 °C to +120 °C, and the tubes are autoclavable when required.

250 mL centrifuge tubes are ideal for process volumes around 100–240 mL, reducing the number of runs compared to 50 mL tubes while remaining easier to handle than 500 mL formats.

 

What we offer at MBP:

 

  • 250ml Tubes with Leak-Proof Caps – High-clarity PP vessels featuring molded graduations for precise volume tracking.

  • Tubes with Bi-directional Transfer Caps – Integrated silicone tubing for secure, closed-system fluid handling.

  • Gamma-Irradiated Sterile Tubes – Ready-to-use containers validated for sensitive biopharmaceutical workflows.

  • Autoclavable Conical Vessels – Durable labware capable of repeated sterilization without loss of function.

  • RNase/DNase Free Laboratory Tubes – Certified protection for nucleic acids during critical molecular preparations.

  • High-RCF Bioprocessing Tubes – Reinforced geometry engineered to maintain seals under intense centrifugal forces.

 

How to Choose 250 mL Centrifuge Tubes

 

Rotor Compatibility and Support Cushions

These tubes require a compatible swing-bucket rotor system designed for their size, along with PEI support cushions. The tube footprint is approximately 60 mm in diameter and 160 mm in height, and many preparative centrifuge systems from major manufacturers support this format with the correct adapters. It is essential to confirm rotor and cushion compatibility before use, as improper support can lead to tube deformation or failure during centrifugation.

 

Sterility Requirements

Sterile 250 mL centrifuge tubes are gamma-irradiated to a sterility assurance level (SAL) of 10⁻⁶ and are certified RNase-free, DNase-free, and non-pyrogenic. These are required for applications involving cell culture harvest, viral vector production, and primary cell isolation. Non-sterile tubes are suitable for chemical processing, precipitation steps, and downstream purification workflows where sterility is not necessary.

 

Graduations and Measurement

These tubes typically include molded graduations that remain readable even after autoclaving or repeated handling. The graduations allow approximate volume measurement without removing the tube from the centrifuge bucket, typically in larger increments suitable for high-volume processing workflows.

 

Pack Quantity and Ordering

250 mL centrifuge tubes are commonly supplied in packs of 5 tubes per bag, with 14 bags per case, totaling approximately 70 tubes per case. Because of moderate case sizes and frequent usage in process labs, many facilities plan short reorder cycles or maintain standing purchase orders to ensure uninterrupted supply.

 

250 mL Centrifuge Tube Specifications

 

250 mL centrifuge tubes are manufactured from high-clarity polypropylene (PP) resin and are designed for compatibility with a wide range of laboratory applications. They are supplied with HDPE plug-seal caps that provide secure closure and leak resistance up to approximately 95 kPa (14 psi), supporting safe handling of biohazardous materials.

These tubes are sterile when required, achieved through gamma irradiation in compliance with ISO 11137 standards (SAL 10⁻⁶). They are certified RNase-free, DNase-free, and non-pyrogenic. The working temperature range is approximately −80 °C to +120 °C, making them suitable for both cold storage and autoclave sterilization workflows.

As of 2026, MBP supplies 250 mL large volume conical centrifuge tubes, and availability may vary by brand and lot. Support cushions are required accessories for proper rotor operation and must be confirmed compatible with the centrifuge system prior to ordering.

Contact the Molecular Biology Products Inc. team for a technical quote today to equip your lab with precision tools.

FAQ

250 mL polypropylene conical centrifuge tubes are rated to 6,000–7,500 × g depending on the manufacturer, in a fully supported swing-bucket rotor with polyetherimide cushions matched to the 60 mm tube diameter. Without cushions, the tube sidewall collapses under centrifugal force.
250 mL conical tubes (60 mm OD × 160 mm height) are compatible with preparative swing-bucket rotors that accept 250 mL adapters, such as the Beckman Coulter JS-5.3, Thermo Fisher TX-750, and equivalent platforms. Confirm adapter and cushion part numbers against your centrifuge documentation before ordering.
MBP 250 mL centrifuge tubes are available sterile, gamma-irradiated to SAL 10⁻⁶ per ISO 11137, and certified RNase-free, DNase-free, and non-pyrogenic. Sterile tubes are required for viral vector production, vaccine concentration, and primary cell culture harvest workflows.
250 mL centrifuge tubes are packed 5 per sterile bag and sold in cases of 14 bags (70 tubes per case) — a higher per-case count than 500 mL tubes (40 per case). Labs running 10–20 harvests per month should plan 2-week reorder cycles or set up a standing PO with MBP.
Choose 250 mL tubes when your process volume per centrifuge run is 100–240 mL — the 250 mL format reduces overfill risk compared with a 500 mL tube at low fill volume, and the 60 mm diameter fits a wider range of preparative rotor adapters than the 95 mm 500 mL format. Use 500 mL when volumes reach 300–480 mL per tube.
250 mL polypropylene conical centrifuge tubes require polyetherimide (PEI) support cushions in the rotor bucket unless the rotor has V-bottom holders matched to the 60 mm tube diameter. Cushions are sold separately — confirm the correct part number for your rotor before the first use.
250 mL centrifuge tubes are used in bench-to-pilot-scale cell culture harvest (150–240 mL volumes), AAV and lentiviral vector concentration, density-gradient preparation, large-volume protein precipitation, and batch clarification steps in bioproduction workflows where 50 mL conicals require six or more repetitions.
MBP accepts institutional purchase orders for 250 mL centrifuge tubes and all consumables. MBP is a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. Contact the MBP team to set up a PO account or standing order, or use the Quick Order portal for repeat purchases.
Polypropylene 250 mL centrifuge tubes are thermally stable from −80 °C to +120 °C and are suitable for frozen sample storage and freeze-thaw cycling. The HDPE plug-seal cap maintains its seal integrity across freeze-thaw at −80 °C without cracking or leaking.
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