Name: Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube
SKU: C60622-EFB050A
Availability: InStock

Centrifuge Tubes

<h2>What Are Centrifuge Tubes?</h2>
 Centrifuge tubes are cylindrical laboratory containers with caps that are used to separate, pellet, and store biological or chemical samples through centrifugal force. They are commonly made from polypropylene (PP) or polystyrene (PS) and are available in sizes ranging from small 0.5 mL microtubes to larger 50 mL conical tubes. These tubes are widely used for cell pelleting, protein precipitation, DNA and RNA extraction, tissue homogenate clarification, and long-term sample storage. Depending on the material, they can withstand temperatures from &minus;80 &deg;C to +121 &deg;C.
In short, the best centrifuge tube depends on your centrifuge rotor, required g-force, sample volume, and sterility needs. 
<h2>What You Will Find: </h2>
<ul>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes" title="All Centrifuge Tubes">All Centrifuge Tubes</a>: Comprehensive selection of 15 mL and 50 mL conical tubes with clear graduations for daily lab workflows.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/oak-ridge-style-centrifuge-tubes" title="Oak Ridge Style Centrifuge Tubes">Oak Ridge Style Centrifuge Tubes</a>: High-speed, chemical-resistant tubes designed to withstand extreme RCF without deformation.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/centrifuge-bottles" title="Centrifuge Bottles">Centrifuge Bottles</a>: Heavy-duty, larger-capacity vessels engineered for bulk sample processing and high-velocity runs.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes" title="Large Volume Centrifuge Tubes">Large Volume Centrifuge Tubes</a>: Industrial-strength 250 mL and 500 mL tubes optimized for pilot-scale harvesting and bioprocessing.</li>
</ul>
&nbsp;
<h2>How to Choose Centrifuge Tubes</h2>
 Volume and Rotor Compatibility
Select a tube size that matches both your sample volume and centrifuge rotor. Microtubes ranging from 0.5&ndash;2 mL are commonly used in microcentrifuges and can typically handle speeds up to 20,000 &times; g. Midi tubes in the 5&ndash;10 mL range are suitable for intermediate sample volumes, while 15 mL and 50 mL conical tubes are commonly used in clinical, research, and preparative centrifuges.
Material: Polypropylene vs. Polystyrene
Polypropylene (PP) tubes offer excellent chemical resistance and can tolerate temperatures from &minus;80 &deg;C to +121 &deg;C. They are suitable for autoclaving and exposure to many laboratory solvents. Polystyrene (PS) tubes provide superior optical clarity, making it easier to visually inspect samples and liquid levels, but they have lower resistance to solvents and extreme temperatures.
Sterility and Contamination Control
Sterile centrifuge tubes are typically gamma- or E-beam-irradiated and certified free of RNase, DNase, and pyrogens. These tubes are recommended for RNA extraction, cell culture, and clinical applications where contamination must be minimized. Non-sterile tubes may still be RNase- and DNase-free and are often suitable for routine DNA work and general sample storage.
Cap Design
Attached screw caps help prevent cap mix-ups and sample contamination, especially in high-throughput laboratories. Separate caps can be quicker to open and may be preferred in workflows that require frequent access to samples. If tubes will be used with automated systems, cap dimensions should be verified for compatibility with liquid handling equipment.
Packaging Options
Racked tubes provide convenient organization and allow direct pipetting while the tubes remain in the rack. Bulk-bagged tubes offer a more economical option for laboratories that process large volumes of samples and already have tube storage systems in place.
<h2> Comparing Common Centrifuge Tube Types</h2>
 Microtubes (0.5&ndash;2 mL) are ideal for PCR preparation, microcentrifuge applications, and storage of small sample volumes. They can generally withstand centrifugal forces up to 20,000 &times; g.
Midi tubes (5&ndash;10 mL) are designed for intermediate sample volumes such as culture supernatants and can typically tolerate forces up to 16,000 &times; g.
Sterile 15 mL conical tubes are frequently used for cell pelleting, RNA extraction, and clinical sample processing, with typical ratings between 12,000 and 15,000 &times; g.
Sterile 50 mL conical tubes are commonly selected for larger-volume applications including tissue culture work, density gradient preparation, and large-scale sample processing.
Polystyrene conical tubes in 15 mL and 50 mL formats are often chosen when visual sample inspection is important and solvent exposure is minimal.
<h2> Specifications and Features</h2>
 Polypropylene centrifuge tubes from Globe Scientific and Nest Scientific are manufactured from USP Class VI virgin resin and do not contain plasticizers or processing additives. Sterile products are typically irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶. Most 15 mL tubes feature graduation marks at 1 mL intervals, while 50 mL tubes are usually graduated every 5 mL. MBP offers both bagged and racked centrifuge tube formats, including 15 mL and 50 mL conical tubes as well as smaller microtube and midi tube options.
MBP supports procurement departments by accepting purchase orders and offering institutional pricing for qualified high-volume customers. <a href="https://mbpinc.net/contact-us">Contact the MBP team today</a> for a customized quote.

<h2 data-section-id="155qizo" data-start="0" data-end="33">What Are All Centrifuge Tubes?</h2>
&nbsp;
The All Centrifuge Tubes collection includes the complete range of tube sizes commonly used in research, clinical, and laboratory environments. These tubes range from small 0.5 mL and 1.5&ndash;2 mL microtubes used in microcentrifuge applications to 5 mL and 10 mL midi tubes for intermediate sample volumes, as well as 15 mL and 50 mL conical tubes designed for cell culture, density gradient preparation, and larger-volume sample processing. They are available in polypropylene (PP) for enhanced chemical resistance and temperature tolerance or polystyrene (PS) for improved optical clarity.
&nbsp;
What you will find:
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microcentrifuge-tubes" title="Microcentrifuge Tubes">Microcentrifuge Tubes</a> &ndash; Precision-engineered for small-volume sample preparation.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/screw-cap-tubes" title="Screw Cap Tubes">Screw Cap Tubes</a> &ndash; Secure, leak-proof closures for long-term storage and transport.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/15ml-and-50ml-centrifuge-tubes" title="15ml &amp; 50ml Centrifuge Tubes">15ml &amp; 50ml Centrifuge Tubes</a> &ndash; Standard conical bottom tubes for large-volume processing.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/midicentrifuge-tubes-5ml-and-10ml" title="Midicentrifuge Tubes (5ml &amp; 10ml)">Midicentrifuge Tubes (5ml &amp; 10ml)</a> &ndash; The perfect bridge for intermediate sample sizes.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microtubes-box" title="Microtubes Box">Microtubes Box</a> &ndash; Durable storage racks for organized sample management and protection.
</li>
</ul>
&nbsp;
<h2 data-section-id="1f93yys" data-start="914" data-end="963">How to Choose From All Centrifuge Tube Formats</h2>
&nbsp;
Volume and Rotor Compatibility
Selecting the correct tube size is essential for safe and efficient centrifugation. Microtubes ranging from 0.5&ndash;2 mL are designed for microcentrifuges, while 5&ndash;10 mL midi tubes fit compatible benchtop centrifuge rotors. Larger 15 mL and 50 mL conical tubes are commonly used in clinical and research centrifuges. Always verify rotor compatibility before introducing a new tube format into your workflow.
Sterility Requirements
For sensitive applications such as RNA extraction, cell culture, and clinical diagnostics, sterile tubes certified free of RNase, DNase, and pyrogens are recommended. Non-sterile tubes can provide a cost-effective solution for routine DNA analysis, protein assays, and general sample storage where sterility is not critical.
Material Selection
Polypropylene tubes offer excellent durability, can be autoclaved at 121 &deg;C, and resist many laboratory chemicals and solvents. Polystyrene tubes provide superior transparency, making it easier to monitor liquid levels and sample contents, although they are less resistant to heat and solvents.
Packaging and Inventory Planning
Microtubes are commonly available in pack sizes ranging from 500 to 1,000 units, while 15 mL and 50 mL conical tubes are often supplied in cases of 500. Laboratories with consistent usage may benefit from establishing recurring purchase orders to ensure uninterrupted supply.
&nbsp;
<h2 data-section-id="urfo3f" data-start="2395" data-end="2433">Compare Centrifuge Tube Size Groups</h2>
&nbsp;
Microtubes (0.5&ndash;2 mL) are primarily used for PCR preparation, microcentrifuge applications, and storage of small sample volumes. These are typically manufactured from polypropylene and are available in pack sizes of 500&ndash;1,000 units.
Midi tubes (5&ndash;10 mL) support intermediate-volume centrifugation and supernatant collection. They are commonly made from polypropylene and supplied in packs ranging from 100&ndash;500 units.
15 mL conical tubes are widely used for cell pelleting, RNA isolation, and clinical sample processing. They are available in both polypropylene and polystyrene formats and are generally packaged in cases of 500.
50 mL conical tubes are designed for larger-volume workflows, including tissue culture, density gradient preparation, and sample processing. Like 15 mL tubes, they are available in polypropylene and polystyrene and are commonly sold in cases of 500.
&nbsp;
<h2 data-section-id="1ezckh3" data-start="3318" data-end="3348">Specifications and Features</h2>
&nbsp;
Globe Scientific and Nest Scientific centrifuge tubes are manufactured from USP Class VI virgin polypropylene or polystyrene resin and are certified free from detectable RNase, DNase, and pyrogens. Sterile products are irradiated using gamma or E-beam methods to achieve a sterility assurance level (SAL) of 10⁻⁶. MBP offers both racked and bagged formats for 15 mL and 50 mL conical tubes, along with dispenser-box options for selected microtube product lines that support efficient use in high-throughput laboratory environments.
For procurement teams, MBP accepts purchase orders and offers institutional pricing for bulk and high-volume purchases. <a href="https://mbpinc.net/contact-ushttps://mbpinc.net/contact-us" title="Contact the MBP team" style="color: #236fa1;">Contact the MBP team</a> today to learn more

All Centrifuge Tubes

<h2 data-section-id="hcowtr" data-start="0" data-end="45">What Are 15 mL and 50 mL Centrifuge Tubes?</h2>
&nbsp;
15 mL and 50 mL centrifuge tubes are conical-bottom vessels in polypropylene (PP) or polystyrene (PS), the two most commonly used tube formats in research and clinical laboratories worldwide. The 15 mL tube is the standard for cell pelleting, RNA isolation, small-scale protein purification, and clinical sample preparation. The 50 mL tube handles larger culture volumes, gradient preparation, tissue homogenate clarification, and bulk precipitation steps. Both formats from Globe Scientific and Nest Scientific are rated to 12,000&ndash;15,000 &times; g in a supported rotor.
15 mL for cell and molecular workflows under 12 mL; 50 mL for culture volumes, gradients, and any prep where a 15 mL conical runs out of headroom.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Attached flat top screw cap: Leak-proof, one-hand use</li>
<li>Racked: Pre-organized for high-throughput</li>
<li>Self-standing: Stable skirted base</li>
<li>Sterile: For contamination-sensitive work</li>
<li>Non-sterile: General, cost-effective use</li>
<li dir="ltr" role="presentation">Bulk: High-volume, economical</li>
<li dir="ltr" role="presentation">Racked: Organized &amp; ready-to-use</li>
<li dir="ltr" role="presentation">PS racked: Extra stability with polystyrene racks</li>
</ul>
&nbsp;

<h2 data-section-id="1j0vxh6" data-start="772" data-end="821">How to Choose 15 mL and 50 mL Centrifuge Tubes</h2>
&nbsp;
Material: PP vs PS
Polypropylene tubes (Globe Scientific, Nest Scientific) are resistant to most organic solvents, stable from &minus;80 &deg;C to +121 &deg;C, and autoclavable. Polystyrene tubes offer greater optical clarity &mdash; the tube wall is transparent enough to read the sample meniscus and assess pellet quality without removing the tube from the rack. Choose PS when visual inspection is a workflow step; choose PP for all other applications.
Sterility Grade
Sterile tubes (gamma- or E-beam-irradiated, RNase-free, DNase-free, non-pyrogenic) are required for RNA work, primary cell culture, and clinical sample handling. Non-sterile, DNase/RNase-free tubes are appropriate and more cost-effective for DNA extractions, protein assays, and general preparative spins.
Cap Style: Flat-Top vs Plug-Seal
Flat-top screw caps (high-density polyethylene, HDPE) provide a flat writing surface for sample labeling and a double-threaded design that reduces cross-threading in high-throughput workflows. Plug-seal screw caps (HDPE or PP) create a secondary internal seal for leak-prone applications. Attached caps come pre-assembled on sterile tubes; separate caps are standard on non-sterile tubes.
Pack Format: Racked vs Bagged
Racked tubes (foam or PS rack, 50 &times; 15 mL or 25 &times; 50 mL per rack, 500 per case) allow direct pipetting without removing tubes and enable viewing of graduations without disturbing the rack. Bagged tubes (25 or 50 per bag, 500 per case) offer a lower cost per tube and suit labs with robotic liquid handlers or in-house racking systems.
&nbsp;
<h2 data-section-id="up19i6" data-start="2392" data-end="2431">Compare 15 mL and 50 mL Tube Options</h2>
&nbsp;
Globe Scientific 15 mL polypropylene tubes with flat-top blue caps are available in both sterile and non-sterile formats with attached or separate caps. These tubes can be purchased in racked or bagged packaging configurations, with 500 tubes per case, and are rated to withstand centrifugal forces up to 15,000 &times; g.
Globe Scientific 15 mL polystyrene tubes with red caps provide enhanced optical clarity for visual sample inspection and are available in sterile and non-sterile versions. They are supplied with attached or separate caps, offered in racked or bagged formats, and rated up to 12,000 &times; g.
Globe Scientific 50 mL polypropylene tubes with flat-top blue caps are designed for larger-volume centrifugation workflows. Available in sterile and non-sterile configurations with attached or separate caps, these tubes are supplied in racked or bagged packaging and can withstand centrifugal forces up to 15,000 &times; g.
Globe Scientific 50 mL polystyrene tubes with red caps combine large-volume capacity with excellent transparency for easy sample visualization. They are available in sterile and non-sterile formats, supplied with attached or separate caps, packaged either racked or bagged, and rated up to 12,000 &times; g.
Nest Scientific 15 mL polypropylene tubes are supplied sterile with attached caps and packaged in polystyrene racks containing 500 tubes per case. These tubes are rated to 12,000 &times; g and are commonly used in cell culture, clinical, and molecular biology applications.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3950" data-end="3975">Specifications Context</h2>
&nbsp;
Globe Scientific 15 mL and 50 mL centrifuge tubes are molded from USP Class VI virgin polypropylene or polystyrene, certified RNase-free, DNase-free, and non-pyrogenic by lot. The 15 mL PP tube is rated to 15,000 &times; g; 50 mL PP tubes to 15,000 &times; g in a supported rotor. Graduated markings on 15 mL tubes read every 1 mL (with sub-milliliter graduations below 1 mL); 50 mL tubes every 5 mL. Globe Scientific is ISO 9001:2015 certified. As of 2026, MBP stocks flat-top (blue cap) and plug-seal (red cap) variants in PP and PS, with sterile and non-sterile options available.
Ensure your high-speed separations remain consistent and safe by choosing tubes built for extreme RCF ratings. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the Molecular Biology Products Inc. team</a> for a quote.

15ml & 50ml Centrifuge Tubes

<h2 data-section-id="kb9bww" data-start="0" data-end="42">What Are Large Volume Centrifuge Tubes?</h2>
&nbsp;
Large volume centrifuge tubes are polypropylene conical-bottom vessels available in 250 mL and 500 mL formats, designed for preparative and process-scale centrifugation at speeds of up to 6,000&ndash;7,500 &times; g in compatible swing-bucket or fixed-angle rotors. These tubes are used for applications such as cell harvesting from bioreactors, viral vector and vaccine production, large-scale protein precipitation, gradient preparation, and clarification of culture supernatants where 50 mL tubes are insufficient.
Polypropylene construction provides resistance to most organic solvents, stability across a temperature range of &minus;80 &deg;C to +120 &deg;C, and full autoclavability for sterile processing workflows.
Use 250 mL or 500 mL centrifuge tubes for process-scale volumes above 50 mL when compatible large-capacity rotors and support systems are available.
&nbsp;
<h2 dir="ltr">This section contains:</h2>
&nbsp;
<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/250ml-centrifuge-tubes" title="250mL Centrifuge Tubes">250mL Centrifuge Tubes </a>&ndash; Optimized for pilot-scale harvesting and efficient mid-range sample concentration.
<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/500ml-centrifuge-tubes" title="500mL Centrifuge Tubes">500mL Centrifuge Tubes</a> &ndash; Industrial-strength vessels designed for bulk bioprocessing and high-capacity separations.
&nbsp;
<h2 data-section-id="1riycx1" data-start="904" data-end="950">How to Choose Large Volume Centrifuge Tubes</h2>
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250 mL vs 500 mL
Choose 250 mL tubes when process volumes per tube run 150&ndash;240 mL &mdash; overfilling beyond ~80% of rated volume risks spill and cap failure. 500 mL tubes are used for volumes from 300&ndash;480 mL, common in vaccine and viral vector production, large-scale mammalian cell culture harvest, and preparative density-gradient centrifugation. The 500 mL tube has a larger diameter (95 mm) and shorter height (146 mm) than the 250 mL tube (60 mm &times; 160 mm), requiring a different rotor adapter.
Support Cushions and Rotor Compatibility
Large volume centrifuge tubes require properly fitted rotor support cushions (typically made from polyetherimide or similar materials) unless the rotor is specifically designed for direct tube support. These cushions prevent tube wall deformation under centrifugal force. Without correct support, tube failure may occur. Compatibility between tubes, cushions, and rotor buckets must always be confirmed before use.
Cap Type: Plug-Seal vs Flat Screw Cap
Plug-seal screw caps made from high-density polyethylene (HDPE) provide an internal sealing mechanism that improves leak resistance during high-force centrifugation, especially with viscous or biohazardous materials. Flat screw caps are generally used for lower-risk applications involving mixing or storage. Large volume polypropylene tubes are typically supplied with plug-seal caps rated for reliable containment under pressure conditions.
Sterility and Certification
Sterile large volume centrifuge tubes are gamma-irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶ and are required for applications such as viral vector production, vaccine manufacturing, and primary cell culture workflows. Non-sterile RNase-free and DNase-free tubes are suitable for chemical processing steps and downstream analytical procedures where sterility is not critical.
&nbsp;
<h2 data-section-id="1xe8cvr" data-start="3019" data-end="3066">Compare Large Volume Centrifuge Tube Formats</h2>
&nbsp;
250 mL polypropylene conical centrifuge tubes are designed for intermediate process-scale applications. These tubes typically measure around 60 mm in diameter and 160 mm in height and are rated for centrifugal forces up to approximately 6,000&ndash;7,500 &times; g. They are commonly supplied sterile with HDPE plug-seal caps and are packaged in bags, often in case quantities of around 70 tubes per case.
500 mL polypropylene conical centrifuge tubes are intended for large-scale processing applications involving high culture volumes or bulk precipitation workflows. These tubes typically measure around 95 mm in diameter and 146 mm in height and are rated up to approximately 6,000 &times; g. They are supplied sterile with HDPE plug-seal caps and are commonly packaged in case quantities of around 40 tubes per case.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3881" data-end="3906">Specifications Context</h2>
&nbsp;
Large volume polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified sterile, RNase-free, DNase-free, and pyrogen-free. Sterile lots are typically gamma-irradiated to SAL 10⁻⁶ in accordance with ISO 11137 standards. Molded graduations allow approximate volume estimation without removing tubes from the centrifuge rotor.
As of 2026, MBP stocks both 250 mL and 500 mL large volume centrifuge tube formats. Support cushions and rotor-specific accessories are typically required and must be verified for compatibility prior to ordering.
Ready to secure your high-volume runs? <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Reach out to the Molecular Biology Products Inc. team</a> for a custom quote today.

Large Volume Centrifuge Tubes

<h2>What Are 250 mL Centrifuge Tubes?</h2>
&nbsp;
250 mL centrifuge tubes are large-volume polypropylene conical tubes designed for processing sample volumes that exceed the capacity of standard 50 mL conical tubes. They are commonly used for cell culture harvests, density-gradient separations, protein precipitation, viral workflows, and other preparative laboratory applications.
These tubes provide an efficient solution for handling medium- to large-scale samples while remaining easier to manage than 500 mL formats. Most models feature leak-resistant caps, molded graduations, and compatibility with swing-bucket centrifuge rotors designed for large-volume processing.
&nbsp;
<h2>What we offer at MBP:</h2>
&nbsp;
<ul>
<li>250 mL centrifuge tubes with leak-proof caps and molded graduations</li>
<li>Tubes with transfer-cap configurations for closed-system fluid handling</li>
<li>Gamma-irradiated sterile tubes for biopharmaceutical and cell culture applications</li>
<li>Autoclavable polypropylene vessels for repeated sterilization cycles</li>
<li>RNase-free and DNase-free tubes for molecular biology workflows</li>
<li>Large-volume centrifuge tubes designed for demanding processing applications</li>
</ul>
&nbsp;
<h2>How to Choose 250 mL Centrifuge Tubes</h2>
&nbsp;
Rotor Compatibility Verify that your centrifuge system supports 250 mL tubes and any required adapters or support cushions. Proper rotor compatibility is essential for safe and reliable operation during centrifugation.
Sterility Requirements Sterile, gamma-irradiated tubes are recommended for cell culture, viral, and sensitive biological applications. Non-sterile options are suitable for general laboratory processing and chemical workflows where sterility is not required.
Graduations and Visibility Molded graduations provide convenient approximate volume measurements and should remain visible after repeated use and sterilization procedures.
Packaging and Supply Planning Large-volume centrifuge tubes are often supplied in moderate case quantities. Laboratories with frequent processing requirements may benefit from maintaining regular inventory levels to avoid workflow interruptions.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
250 mL centrifuge tubes are typically manufactured from high-clarity polypropylene and fitted with secure, leak-resistant caps for safe sample handling. Many products are autoclavable and suitable for temperature ranges commonly encountered in laboratory storage and sterilization procedures.
Sterile versions are available with certifications such as RNase-free, DNase-free, and non-pyrogenic status to support molecular biology and bioprocessing applications. Proper accessories, including compatible rotor cushions and adapters, should always be verified before use.
As of 2026, MBP offers large-volume centrifuge tubes for research, clinical, and bioprocessing laboratories. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for product information and technical support.

250ml Centrifuge Tubes

<h2 data-section-id="57m9pt" data-start="0" data-end="36">What Are 500 mL Centrifuge Tubes?</h2>
&nbsp;
500 mL centrifuge tubes are large-format polypropylene conical vessels used for process-scale centrifugation applications where sample volumes exceed approximately 300 mL per tube. These tubes typically have standard dimensions of 95 mm outer diameter and 146 mm height, and feature a conical bottom that enables efficient pellet formation and recovery. They are rated for use up to 6,000 &times; g in a compatible swing-bucket rotor system equipped with polyetherimide (PEI) support cushions.
These tubes are commonly used in large-scale mammalian cell culture harvesting from bioreactors, viral vector production (including AAV and lentivirus), vaccine manufacturing workflows, large-volume density-gradient separations, and preparative-scale protein or nucleic acid precipitation.
500 mL centrifuge tubes are used when process volumes are 300&ndash;480 mL per run and a 250 mL tube would require multiple centrifugation cycles.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">The MBP Quality Standard:</h2>
&nbsp;
<ul>
<li dir="ltr">High-capacity 500ml vessels for industrial-strength bulk concentration.</li>
<li dir="ltr">Bi-directional transfer caps with silicone tubing for closed-system fluid handling.</li>
<li dir="ltr">Gamma-irradiated sterile containers validated for aseptic processing.</li>
<li dir="ltr">Chemical-resistant construction for durability against aggressive buffers.</li>
</ul>
&nbsp;
<h2 data-section-id="1njhdwk" data-start="971" data-end="1011">How to Choose 500 mL Centrifuge Tubes</h2>
&nbsp;
Rotor Compatibility First
500 mL centrifuge tubes require a compatible swing-bucket rotor system that can accommodate a 95 mm tube diameter along with appropriate PEI support cushions. Common compatible systems include Thermo Fisher TX-750 and equivalent swing-bucket rotor models. It is essential to confirm both rotor bucket compatibility and correct cushion part numbers before use.
Using 500 mL tubes without proper support cushions or in incompatible rotors can lead to tube deformation or failure even at relatively low centrifugal forces.
Sterility for Bioproduction Workflows
Sterile 500 mL centrifuge tubes are gamma-irradiated to a sterility assurance level (SAL) of 10⁻⁶ and are certified RNase-free, DNase-free, and non-pyrogenic. These are required for bioproduction applications such as viral vector manufacturing, vaccine production, and primary cell culture workflows.
Non-sterile tubes may be used for downstream processing steps, chemical precipitation, or other laboratory applications where sterility is not required.
Cap Integrity and Leak Prevention
500 mL tubes are equipped with HDPE plug-seal screw caps that create an internal compression seal within the tube neck. This design provides leak resistance up to 95 kPa (14 psi), which is important for handling biohazardous or high-risk samples such as BSL-2 cell lines or viral vectors.
Proper cap closure is critical before centrifugation; caps must be fully tightened without cross-threading to ensure safe operation.
Pack Size and Ordering Considerations
These tubes are typically supplied in packs of 5 tubes per bag and 8 bags per case, totaling 40 tubes per case. Because of the relatively low number of tubes per case compared to smaller formats, laboratories performing high-throughput bioprocessing often maintain standing purchase orders to avoid supply interruptions.
&nbsp;
<h2 data-section-id="1hsndwv" data-start="2893" data-end="2933">Specifications Context</h2>
&nbsp;
500 mL polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified RNase-free, DNase-free, and non-pyrogenic. Sterilization is performed via gamma irradiation in compliance with ISO 11137 standards, achieving a sterility assurance level of 10⁻⁶. Molded graduations on the tube body allow approximate volume measurement without removing the tube from the centrifuge rotor.
As of 2026, MBP supplies 500 mL large volume centrifuge tubes; availability, brand options, and lot-specific details may vary. Support cushions are required for safe operation and must be confirmed as compatible with the selected rotor system prior to ordering.
Secure your research and your results. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> for a quote.

500ml Centrifuge Tubes

Product Literature

Centrifuge Tube Closed System Solution

Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube

Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube by NEST Scientific — 50 mL centrifuge tube with bi-directional transfer cap, silicone tube (1/8&quot; ID &times; 1/4&quot; OD), male Luer Lock connector, 0.22 &mu;m &times; 24 mm vent filter. Sterile, 1/pk, 4/cs. Available in 50mL (C60622-EFB050A), 250mL (C60922-EFB050A), and 500mL (C61022-EFB050A).

Manufacturer

50mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

250mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

500mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs