Name: Large Volume Centrifuge Tubes
SKU: 6334
Availability: InStock

Large Volume Centrifuge Tubes

<h2 data-section-id="kb9bww" data-start="0" data-end="42">What Are Large Volume Centrifuge Tubes?</h2>
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Large volume centrifuge tubes are polypropylene conical-bottom vessels available in 250 mL and 500 mL formats, designed for preparative and process-scale centrifugation at speeds of up to 6,000&ndash;7,500 &times; g in compatible swing-bucket or fixed-angle rotors. These tubes are used for applications such as cell harvesting from bioreactors, viral vector and vaccine production, large-scale protein precipitation, gradient preparation, and clarification of culture supernatants where 50 mL tubes are insufficient.
Polypropylene construction provides resistance to most organic solvents, stability across a temperature range of &minus;80 &deg;C to +120 &deg;C, and full autoclavability for sterile processing workflows.
Use 250 mL or 500 mL centrifuge tubes for process-scale volumes above 50 mL when compatible large-capacity rotors and support systems are available.
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<h2 dir="ltr">This section contains:</h2>
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<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/250ml-centrifuge-tubes" title="250mL Centrifuge Tubes">250mL Centrifuge Tubes </a>&ndash; Optimized for pilot-scale harvesting and efficient mid-range sample concentration.
<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/500ml-centrifuge-tubes" title="500mL Centrifuge Tubes">500mL Centrifuge Tubes</a> &ndash; Industrial-strength vessels designed for bulk bioprocessing and high-capacity separations.
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<h2 data-section-id="1riycx1" data-start="904" data-end="950">How to Choose Large Volume Centrifuge Tubes</h2>
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250 mL vs 500 mL
Choose 250 mL tubes when process volumes per tube run 150&ndash;240 mL &mdash; overfilling beyond ~80% of rated volume risks spill and cap failure. 500 mL tubes are used for volumes from 300&ndash;480 mL, common in vaccine and viral vector production, large-scale mammalian cell culture harvest, and preparative density-gradient centrifugation. The 500 mL tube has a larger diameter (95 mm) and shorter height (146 mm) than the 250 mL tube (60 mm &times; 160 mm), requiring a different rotor adapter.
Support Cushions and Rotor Compatibility
Large volume centrifuge tubes require properly fitted rotor support cushions (typically made from polyetherimide or similar materials) unless the rotor is specifically designed for direct tube support. These cushions prevent tube wall deformation under centrifugal force. Without correct support, tube failure may occur. Compatibility between tubes, cushions, and rotor buckets must always be confirmed before use.
Cap Type: Plug-Seal vs Flat Screw Cap
Plug-seal screw caps made from high-density polyethylene (HDPE) provide an internal sealing mechanism that improves leak resistance during high-force centrifugation, especially with viscous or biohazardous materials. Flat screw caps are generally used for lower-risk applications involving mixing or storage. Large volume polypropylene tubes are typically supplied with plug-seal caps rated for reliable containment under pressure conditions.
Sterility and Certification
Sterile large volume centrifuge tubes are gamma-irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶ and are required for applications such as viral vector production, vaccine manufacturing, and primary cell culture workflows. Non-sterile RNase-free and DNase-free tubes are suitable for chemical processing steps and downstream analytical procedures where sterility is not critical.
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<h2 data-section-id="1xe8cvr" data-start="3019" data-end="3066">Compare Large Volume Centrifuge Tube Formats</h2>
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250 mL polypropylene conical centrifuge tubes are designed for intermediate process-scale applications. These tubes typically measure around 60 mm in diameter and 160 mm in height and are rated for centrifugal forces up to approximately 6,000&ndash;7,500 &times; g. They are commonly supplied sterile with HDPE plug-seal caps and are packaged in bags, often in case quantities of around 70 tubes per case.
500 mL polypropylene conical centrifuge tubes are intended for large-scale processing applications involving high culture volumes or bulk precipitation workflows. These tubes typically measure around 95 mm in diameter and 146 mm in height and are rated up to approximately 6,000 &times; g. They are supplied sterile with HDPE plug-seal caps and are commonly packaged in case quantities of around 40 tubes per case.
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<h2 data-section-id="1jl751w" data-start="3881" data-end="3906">Specifications Context</h2>
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Large volume polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified sterile, RNase-free, DNase-free, and pyrogen-free. Sterile lots are typically gamma-irradiated to SAL 10⁻⁶ in accordance with ISO 11137 standards. Molded graduations allow approximate volume estimation without removing tubes from the centrifuge rotor.
As of 2026, MBP stocks both 250 mL and 500 mL large volume centrifuge tube formats. Support cushions and rotor-specific accessories are typically required and must be verified for compatibility prior to ordering.
Ready to secure your high-volume runs? <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Reach out to the Molecular Biology Products Inc. team</a> for a custom quote today.

<h2>What Are 250 mL Centrifuge Tubes?</h2>
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250 mL centrifuge tubes are large-volume polypropylene conical tubes designed for processing sample volumes that exceed the capacity of standard 50 mL conical tubes. They are commonly used for cell culture harvests, density-gradient separations, protein precipitation, viral workflows, and other preparative laboratory applications.
These tubes provide an efficient solution for handling medium- to large-scale samples while remaining easier to manage than 500 mL formats. Most models feature leak-resistant caps, molded graduations, and compatibility with swing-bucket centrifuge rotors designed for large-volume processing.
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<h2>What we offer at MBP:</h2>
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<ul>
<li>250 mL centrifuge tubes with leak-proof caps and molded graduations</li>
<li>Tubes with transfer-cap configurations for closed-system fluid handling</li>
<li>Gamma-irradiated sterile tubes for biopharmaceutical and cell culture applications</li>
<li>Autoclavable polypropylene vessels for repeated sterilization cycles</li>
<li>RNase-free and DNase-free tubes for molecular biology workflows</li>
<li>Large-volume centrifuge tubes designed for demanding processing applications</li>
</ul>
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<h2>How to Choose 250 mL Centrifuge Tubes</h2>
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Rotor Compatibility Verify that your centrifuge system supports 250 mL tubes and any required adapters or support cushions. Proper rotor compatibility is essential for safe and reliable operation during centrifugation.
Sterility Requirements Sterile, gamma-irradiated tubes are recommended for cell culture, viral, and sensitive biological applications. Non-sterile options are suitable for general laboratory processing and chemical workflows where sterility is not required.
Graduations and Visibility Molded graduations provide convenient approximate volume measurements and should remain visible after repeated use and sterilization procedures.
Packaging and Supply Planning Large-volume centrifuge tubes are often supplied in moderate case quantities. Laboratories with frequent processing requirements may benefit from maintaining regular inventory levels to avoid workflow interruptions.
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<h2>Specifications Context</h2>
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250 mL centrifuge tubes are typically manufactured from high-clarity polypropylene and fitted with secure, leak-resistant caps for safe sample handling. Many products are autoclavable and suitable for temperature ranges commonly encountered in laboratory storage and sterilization procedures.
Sterile versions are available with certifications such as RNase-free, DNase-free, and non-pyrogenic status to support molecular biology and bioprocessing applications. Proper accessories, including compatible rotor cushions and adapters, should always be verified before use.
As of 2026, MBP offers large-volume centrifuge tubes for research, clinical, and bioprocessing laboratories. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for product information and technical support.

250ml Centrifuge Tubes

<h2 data-section-id="57m9pt" data-start="0" data-end="36">What Are 500 mL Centrifuge Tubes?</h2>
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500 mL centrifuge tubes are large-format polypropylene conical vessels used for process-scale centrifugation applications where sample volumes exceed approximately 300 mL per tube. These tubes typically have standard dimensions of 95 mm outer diameter and 146 mm height, and feature a conical bottom that enables efficient pellet formation and recovery. They are rated for use up to 6,000 &times; g in a compatible swing-bucket rotor system equipped with polyetherimide (PEI) support cushions.
These tubes are commonly used in large-scale mammalian cell culture harvesting from bioreactors, viral vector production (including AAV and lentivirus), vaccine manufacturing workflows, large-volume density-gradient separations, and preparative-scale protein or nucleic acid precipitation.
500 mL centrifuge tubes are used when process volumes are 300&ndash;480 mL per run and a 250 mL tube would require multiple centrifugation cycles.
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<h2 dir="ltr">The MBP Quality Standard:</h2>
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<ul>
<li dir="ltr">High-capacity 500ml vessels for industrial-strength bulk concentration.</li>
<li dir="ltr">Bi-directional transfer caps with silicone tubing for closed-system fluid handling.</li>
<li dir="ltr">Gamma-irradiated sterile containers validated for aseptic processing.</li>
<li dir="ltr">Chemical-resistant construction for durability against aggressive buffers.</li>
</ul>
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<h2 data-section-id="1njhdwk" data-start="971" data-end="1011">How to Choose 500 mL Centrifuge Tubes</h2>
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Rotor Compatibility First
500 mL centrifuge tubes require a compatible swing-bucket rotor system that can accommodate a 95 mm tube diameter along with appropriate PEI support cushions. Common compatible systems include Thermo Fisher TX-750 and equivalent swing-bucket rotor models. It is essential to confirm both rotor bucket compatibility and correct cushion part numbers before use.
Using 500 mL tubes without proper support cushions or in incompatible rotors can lead to tube deformation or failure even at relatively low centrifugal forces.
Sterility for Bioproduction Workflows
Sterile 500 mL centrifuge tubes are gamma-irradiated to a sterility assurance level (SAL) of 10⁻⁶ and are certified RNase-free, DNase-free, and non-pyrogenic. These are required for bioproduction applications such as viral vector manufacturing, vaccine production, and primary cell culture workflows.
Non-sterile tubes may be used for downstream processing steps, chemical precipitation, or other laboratory applications where sterility is not required.
Cap Integrity and Leak Prevention
500 mL tubes are equipped with HDPE plug-seal screw caps that create an internal compression seal within the tube neck. This design provides leak resistance up to 95 kPa (14 psi), which is important for handling biohazardous or high-risk samples such as BSL-2 cell lines or viral vectors.
Proper cap closure is critical before centrifugation; caps must be fully tightened without cross-threading to ensure safe operation.
Pack Size and Ordering Considerations
These tubes are typically supplied in packs of 5 tubes per bag and 8 bags per case, totaling 40 tubes per case. Because of the relatively low number of tubes per case compared to smaller formats, laboratories performing high-throughput bioprocessing often maintain standing purchase orders to avoid supply interruptions.
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<h2 data-section-id="1hsndwv" data-start="2893" data-end="2933">Specifications Context</h2>
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500 mL polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified RNase-free, DNase-free, and non-pyrogenic. Sterilization is performed via gamma irradiation in compliance with ISO 11137 standards, achieving a sterility assurance level of 10⁻⁶. Molded graduations on the tube body allow approximate volume measurement without removing the tube from the centrifuge rotor.
As of 2026, MBP supplies 500 mL large volume centrifuge tubes; availability, brand options, and lot-specific details may vary. Support cushions are required for safe operation and must be confirmed as compatible with the selected rotor system prior to ordering.
Secure your research and your results. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> for a quote.

500ml Centrifuge Tubes

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Large Volume Centrifuge Tubes by Globe Scientific are gamma-irradiation-sterilized (SAL 10⁻⁶, ISO 11137) PP tubes with attached HDPE plug-seal caps, rated to 7,500 × g; DNase/RNase-free, non-pyrogenic, autoclavable at 121°C, and freezable to −40°C. Available in 225 mL (6334, 48/case), 250 mL (6336, 48/case), and 500 mL (6338, 36/case).