PETG Erlenmeyer Flasks — Single-Use BPA-Free Shake Flasks for Suspension Culture

PETG Erlenmeyer flasks are single-use, BPA-free, sterile shake flasks manufactured from glycol-modified PET, rated from −80 °C to +60 °C, for suspension cell culture of CHO-S, HEK293-F, Sf9, insect cells, yeast, and bacteria on orbital shakers. NEST Scientific PETG flasks are available in 125 mL–1,000 mL with flat-base or baffled-base geometry and 0.22 µm oversized vented or plug seal HDPE caps; E-beam sterilized to SAL 10⁻⁶; ISO 9001:2015 and ISO 13485 certified.

MBP carries the full line with direct PO ordering for labs across the USA, Canada, and worldwide. Contact customerservice@mbpinc.net for further details.

PETG Erlenmeyer Flasks

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Flat Bottom PETG Erlenmeyer Flasks

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What are PETG Erlenmeyer Flasks?

PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are single-use, sterile, BPA-free conical shake flasks manufactured from glycol-modified PET for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. PETG offers optical clarity comparable to PC, a working temperature range of −80 °C to +60 °C, superior impact resistance at low temperatures versus standard PET, and a BPA-free composition — advantages over PC in single-use workflows where autoclaving is not required, and BPA-sensitive cell culture applications are involved. PETG Erlenmeyer flasks are the correct choice for high-throughput single-use suspension culture, BPA-free applications, and labs that want to eliminate autoclave and washing steps in their shake flask workflow.

What you will find:

125mL PETG Erlenmeyer Flasks for Screening Studies: Perfect for small-scale media optimization and high-speed orbital shaking.
250mL PETG Erlenmeyer Flasks for Routine Expansion: Provides a stable environment for consistent cell growth and passage maintenance.
500mL PETG Erlenmeyer Flasks for Batch Production: Optimized for increasing cell densities while maintaining superior gas exchange.
1000mL PETG Erlenmeyer Flasks for Large-Scale Recovery: Engineered for high-volume bioprocessing and bulk reagent preparation.

How to Choose PETG Erlenmeyer Flasks

PETG vs. PC for Shake Flask Culture
PETG flasks are single-use and cannot be autoclaved (maximum temperature +60 °C). PC flasks can be autoclaved 5–10 times and have a broader temperature range (−135 °C to +135 °C). For high-throughput suspension culture operations processing many flasks per week, single-use PETG eliminates all washing, autoclave queuing, and glassware inspection steps — reducing hands-on time and contamination risk from reuse. For labs with lower throughput that prepare media in-house via autoclave, PC remains the cost-effective choice. PETG is BPA-free; PC contains BPA.

Cell Line Compatibility
PETG Erlenmeyer flasks are validated for CHO-S, HEK293-F, Expi293, Jurkat, K562, Sf9 (Spodoptera frugiperda), Hi5 (Trichoplusia ni), S. cerevisiae, P. pastoris, and E. coli suspension culture. The non-treated PETG surface prevents inadvertent cell adhesion and is compatible with all standard suspension culture media, including FreeStyle CHO, Expi293, Sf-900, and ESF 921 insect cell media. PETG is chemically resistant to DMSO, ethanol, isopropanol, and standard aqueous buffers.

Baffled vs. Flat Base for PETG Flasks
Baffled-base PETG Erlenmeyer flasks are the standard for oxygen-demanding suspension cultures: CHO, HEK293-F, bacterial, and insect cell lines. The baffles generate turbulent flow during orbital shaking, raising dissolved oxygen and reducing cell sedimentation. Flat-base PETG flasks are used for media preparation, gentle mixing, and shear-sensitive cell lines where turbulent conditions cause cell damage. NEST Scientific offers baffled and flat-base versions in both 125 mL and 1,000 mL.

Vented Cap Gas Exchange
NEST Scientific PETG Erlenmeyer flasks ship with a 0.22 µm hydrophobic PTFE membrane vented cap in an HDPE housing with an oversized vent area. For suspension culture with 20–25% fill volume, vented caps at standard orbital speeds (100–150 RPM) provide adequate oxygen mass transfer to support mammalian cell densities up to approximately 5 × 10⁶ cells/mL. For cultures targeting higher densities or with very high specific oxygen uptake rates (sOUR), confirm dissolved oxygen by inline monitoring before scaling further in PETG flasks.

Storage of Unused Sterile Flasks
Store unused NEST Scientific PETG Erlenmeyer flasks at room temperature (15–30 °C) in the original sealed sterile bag, away from UV light. Shelf life is typically 3 years from the production date per the lot certificate. Inspect the bag seal before use; a compromised seal invalidates sterility. Remove from the bag inside a biosafety cabinet immediately before inoculation.

Specifications Context

NEST Scientific PETG Erlenmeyer flasks are manufactured from USP Class VI medical-grade PETG in ISO 8 cleanrooms. E-beam sterilized to SAL 10⁻⁶; each lot certified DNase/RNase-free, pyrogen-free, non-cytotoxic. Temperature range: −80 °C to +60 °C. HDPE cap with 0.22 µm PTFE hydrophobic membrane; oversized vent area for improved gas transfer. NEST Scientific is ISO 9001:2015 and ISO 13485 certified; FDA registered. NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL with baffled and flat-base options are stocked by MBP.

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FAQ

PETG Erlenmeyer flasks are single-use, BPA-free, sterile shake flasks for suspension cell culture of CHO-S, HEK293-F, Jurkat, K562, Sf9, Hi5, yeast, and bacterial cultures on orbital shakers. Their PETG construction is rated from −80 °C to +60 °C, provides glass-like clarity, and eliminates autoclave and washing steps compared to reusable PC or glass shake flasks.

PETG Erlenmeyer flasks cannot be autoclaved — PETG deforms irreversibly at temperatures above +60 °C. They are supplied pre-sterilized by E-beam irradiation to SAL 10⁻⁶ as single-use vessels. For shake flask applications requiring in-house autoclave sterilization or reuse, choose PC (polycarbonate) Erlenmeyer flasks, which withstand 5–10 cycles at 121 °C.

PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are BPA-free — BPA is not present in PET or PETG polymer chemistry. This makes PETG flasks the preferred shake flask material for hormone-sensitive cell lines (estrogen receptor–positive), stem cell culture, and any process requiring documentation that no BPA-containing plasticware contacted the culture. PC flasks contain BPA as a monomer consequence.

For active suspension shaker culture, a 500 mL PETG Erlenmeyer flask should be filled to 20–25% of nominal volume — 100–125 mL working volume. Exceeding 125 mL fill reduces headspace oxygen transfer, generates foam that blocks the 0.22 µm vent cap membrane, and increases shear stress from turbulent liquid dynamics. For static media storage with no shaking, fill can approach 80% of nominal volume.

PETG Erlenmeyer flasks are validated for CHO-S (suspension-adapted Chinese Hamster Ovary), HEK293-F, Expi293, Jurkat T-cells, K562, HL-60, Sf9 and Hi5 insect cells, Saccharomyces cerevisiae, Pichia pastoris, and E. coli. The non-treated PETG inner surface is compatible with all suspension culture media — FreeStyle CHO, Expi293, Sf-900 III SFM, ESF 921 — without promoting unwanted cell adhesion.

Standard orbital shaker speeds for mammalian suspension culture in PETG baffled Erlenmeyer flasks are 100–150 RPM with a 25–50 mm orbital throw. Bacterial and yeast cultures in baffled PETG flasks typically require 150–250 RPM for adequate oxygen transfer. Confirm shear tolerance for your specific mammalian cell line before exceeding 150 RPM — above this threshold, shear-sensitive lines may show reduced viability.

Baffled-base PETG flasks have internal angled baffles that disrupt circular rotational flow during orbital shaking, generating turbulence that increases dissolved oxygen transfer by up to 2× compared to flat-base formats at the same RPM. Flat-base PETG flasks produce gentler, lower-shear rotational mixing suited for media preparation, gentle suspension of shear-sensitive cells, and culture stages where turbulence is undesirable.

MBP stocks NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL volumes with flat-base and baffled-base configurations and 0.22 µm vented or plug seal caps, available for PO-based case-quantity ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. Contact MBP for current pricing and available configurations.

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