Brands

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
<li dir="ltr" aria-level="1">
Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
</li>
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Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
</li>
<li dir="ltr" aria-level="1">
Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
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Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
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Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

DataSheet

Product Literature

Cell Culture Flask, Vent Cap, TC Treated Specifications

Cell Culture Flask, Vent Cap, TC, Sterile - Nest Scientific

NEST Scientific 707003 is a 25 cm² TC-treated cell culture flask with HDPE vented cap, made from USP Class VI polystyrene and e-beam sterilized to SAL 10⁻⁶. Working volume 5–10 mL; 200 per case. ISO 13485:2016 and cGMP certified. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

25cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
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<h2 class="p1">What you will find:</h2>
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<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p2">What are TC-Treated Cell Culture Plates?</h2>
 TC-treated (tissue culture&ndash;treated) cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces have been modified by vacuum plasma treatment to introduce negatively charged oxygen-containing groups (carboxyl, carbonyl, hydroxyl), reducing the water contact angle from ~75&ndash;90&deg; (untreated PS) to below 10&deg; (hydrophilic). This surface modification directly enables attachment, spreading, and monolayer formation of adherent mammalian cell lines. TC-treated plates are the default format for all adherent mammalian cultures, cell-based assays, ELISA preparation, compound cytotoxicity screening, transfection, and gene expression studies.&nbsp; 
<h2 class="p1">What you will find:</h2>
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&bull; 6, 12, 24, 48, and 96 well formats for different workflows &bull; Tissue-culture-treated surfaces for reliable cell attachment &bull; Clear plates for easy monitoring under the microscope &bull; Uniform wells for consistent seeding and growth &bull; Sterile options to maintain clean conditions &bull; Lids designed to reduce contamination and evaporation
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<h2 class="p2">How to Choose TC-Treated Cell Culture Plates</h2>
 Well Format by Cell Number and Assay Type 6-well TC-treated plates (9.4 cm&sup2;/well) are the standard for Western blot preparation, siRNA transfection, viral infection, protein overexpression, and colony-forming unit (CFU) assays requiring &ge;3 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) suit medium-scale reporter assays, luciferase quantification, and ELISA from cell lysate. 24-well plates (1.9 cm&sup2;/well) are versatile for cytotoxicity dose-response in 4&ndash;6 conditions per plate, primary cell experiments, and viral plaque assays. 96-well flat-bottom TC-treated plates (0.32 cm&sup2;/well) are the universal format for cell viability (MTS, MTT, CCK-8), compound screening, ELISA, reporter gene assays, and qPCR lysate preparation across 8&ndash;12 concentrations with triplicate wells. 384-well plates reduce reagent cost per compound for HTS drug discovery programs. Lot-to-Lot TC Treatment Consistency For longitudinal drug screening, biomarker validation, or multi-site studies, lot-to-lot variation in TC treatment can alter cell attachment density, proliferation rate, and assay Z-factor. NEST Scientific verifies vacuum plasma treatment by water contact angle goniometry on each manufacturing lot; the release criterion is contact angle &lt;10&deg;. Request lot certificates when adopting TC-treated plates for GLP- or GMP-regulated assay workflows. Low-Evaporation EDGE Plates For 96-well dose-response assays running 72&ndash;96 hours, peripheral (edge) well evaporation can increase medium osmolality in the outer ring by 10&ndash;20% versus inner wells, introducing a systematic bias in IC50 determinations. NEST Low Evaporation EDGE 96-well TC-treated plates use an internal well ridge design that reduces edge-well evaporation during extended incubations. Use EDGE plates for any dose-response study where incubation time exceeds 48 hours. Cell Line Compatibility TC-treated NEST plates support attachment and monolayer culture of: HeLa (cervical), HEK293 (adherent), A549 (lung), MCF-7 (breast), U2OS (bone), CHO-K1 (ovary, adherent), Vero (kidney), MDCK (canine kidney), NIH 3T3 (mouse fibroblast), MRC-5, 2BS, 293T, L-929, and standard primary cultures including dermal fibroblasts, endothelial cells, and epithelial cells. Do NOT use TC-treated plates for Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9, Hi5, or any non-adherent cell line. Plate Reader and Automation Compatibility NEST Scientific TC-treated 96-well flat plates are compatible with all common microplate readers (BioTek Synergy H1, Molecular Devices SpectraMax i3x, PerkinElmer EnVision, BMG Labtech CLARIOstar) for absorbance, fluorescence intensity, and luminescence detection. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS dimensions for automated liquid handlers (Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek i7). 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific TC-treated cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry; the lot release criterion: contact angle &lt;10&deg;. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS footprint. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free, non-pyrogenic per lot certificate. Clear polystyrene lids included; individually wrapped in peelable film. Alphanumeric well coordinates on all &ge;12-well formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, NEST Scientific TC-treated plates across all core well formats (6- through 384-well) are stocked by MBP. 
Use treated plates when attachment drives your results. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to get reliable plates for keeping your cells anchored, healthy, and consistent across every well.&nbsp;

Treated Cell Culture Plates

Cell Culture Plates - Bulk Packaging

703002 by NEST Scientific is a 6-well flat-bottom TC-treated cell culture plate in bulk packaging (10/bag, 50/case). USP Class VI polystyrene, E-beam sterilized to SAL 10⁻⁶, pyrogen/DNase/RNase/BSE/TSE tested, ISO 7 cleanroom manufactured, 9.5 cm² per well, SBS/ANSI footprint. Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Manufacturer

6 Well Cell Culture Plate, flat, TC, sterile, Bulk, 10/pk, 50/cs

12 Well Cell Culture Plate, flat, TC, sterile, Bulk, 10/pk, 50/cs

24 Well Cell Culture Plate, flat, TC, sterile, Bulk, 10/pk, 50/cs

48 Well Cell Culture Plate, flat, TC, sterile, Bulk, 10/pk, 50/cs

96 Well Cell Culture Plate, flat, TC, sterile, Bulk, 10/pk, 100/cs

Cell Culture Plates - Individually plastic wrapped

703003 by NEST Scientific is a sterile 6-well flat-bottom TC-treated cell culture plate, individually plastic wrapped, 50/case. 100% virgin polystyrene, E-beam sterilized at SAL 10⁻⁶, non-pyrogenic, DNase/RNase-free. Low-evaporation lid, microscope-protective bottom feet, well-coordinate markings. For adherent cell culture, transfection, and primary cell expansion.

6 Well Cell Culture Plate, Flat, TC, Sterile, Individually plastic wrapped, 1/pk, 50/cs

12 Well Cell Culture Plate, Flat, TC, Sterile, Individually plastic wrapped, 1/pk, 50/cs

24 Well Cell Culture Plate, Flat, TC, Sterile, Individually plastic wrapped, 1/pk, 50/cs

48 Well Cell Culture Plate, Flat, TC, Sterile, Individually plastic wrapped, 1/pk, 50/cs

96 Well Cell Culture Plate, Flat, TC, Sterile, Individually plastic wrapped, 1/pk, 100/cs

Storage Boxes & Racks

<h2 data-section-id="1hv97bw" data-start="0" data-end="47">What Are Laboratory Storage Boxes and Racks?</h2>
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Laboratory storage boxes and racks are essential organizational systems used to store, organize, and protect cryogenic vials, microcentrifuge tubes, centrifuge tubes, and microscope slides across freezers, cold rooms, and benchtop environments. Storage boxes are typically lidded, grid-based containers used for long-term sample organization in ultra-low temperature conditions, while racks are open-frame supports used for active handling during laboratory workflows such as pipetting, vortexing, and reaction setup.
Cryogenic storage boxes are commonly designed to hold 81 vials (9&times;9 format) or 100 vials (10&times;10 format), and are compatible with storage conditions ranging from &ndash;196 &deg;C in liquid nitrogen vapor phase to +121 &deg;C for autoclave sterilization (depending on material). Tube racks, on the other hand, provide stable upright positioning of tubes during active benchwork and are not primarily intended for long-term freezer storage.
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<h2 dir="ltr">What you will find:</h2>
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Cryogenic storage boxes for vials and microtubes
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Freezer storage boxes for -20&deg;C and -80&deg;C applications
</li>
<li dir="ltr" aria-level="1">
PCR tube racks for 0.2 mL tubes and strips
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Microcentrifuge tube racks for 0.5 mL to 2 mL tubes
</li>
<li dir="ltr" aria-level="1">
Polypropylene (PP) autoclavable storage boxes
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Stackable storage boxes for space-saving organization
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Hinged lid storage boxes for secure sample protection
</li>
<li dir="ltr" aria-level="1">
Color-coded racks for easy sample identification
</li>
</ul>
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<h2 data-section-id="flyrii" data-start="1159" data-end="1199">How to Choose Storage Boxes and Racks</h2>
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Vial Format and Capacity
The first selection criterion is compatibility with tube size and grid layout. The most common format is the 81-place (9&times;9) box designed for 1.0&ndash;2.0 mL cryovials. Smaller or specialized formats may accommodate 5 mL tubes or different vial diameters depending on grid spacing. Proper matching ensures secure fit, easy retrieval, and consistent sample organization in high-density storage systems.
Temperature Range
Material selection determines allowable storage conditions. Polycarbonate cryogenic boxes are the most versatile, supporting temperatures from liquid nitrogen levels (&ndash;196 &deg;C) through &ndash;80 &deg;C freezer storage and up to autoclave sterilization at +121 &deg;C. Cardboard freezer boxes with moisture-resistant coating are designed for economical freezer storage and can withstand ultra-low temperatures, but they are not suitable for autoclaving or long-term moisture exposure.
Tube Rack Materials and Use
Polypropylene racks are widely used because they are autoclavable and chemically resistant, making them suitable for sterile workflows. ABS and polystyrene racks provide lower-cost alternatives for general laboratory bench use but are not designed for autoclave conditions. For freezer organization, stainless steel racks are often used to hold multiple cryoboxes within &ndash;80 &deg;C upright freezer systems, improving accessibility and spatial organization.
Slide Storage Boxes
Microscope slide storage boxes are designed to protect standard 25 &times; 75 mm slides. They are available in 25-, 50-, and 100-slide capacities. Polypropylene versions are autoclavable and suitable for sterilized workflows, while fiberboard or ABS versions are typically used for room-temperature archival storage and transport.
Color Coding and Sample Identification
Color-coded storage boxes are widely used in biobanking and high-throughput laboratories to quickly differentiate sample groups without opening boxes or reading labels at freezer temperatures. Common systems include multiple lid colors paired with alphanumeric grids inside the box, enabling precise sample tracking and retrieval in large inventories.
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<h2 data-section-id="1jl751w" data-start="4067" data-end="4092">Specifications Context</h2>
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Modern polycarbonate cryogenic storage boxes are engineered for extreme temperature stability, typically rated from &ndash;196 &deg;C to +121 &deg;C, allowing use across liquid nitrogen vapor phase storage, &ndash;80 &deg;C freezers, and autoclave sterilization cycles. Cardboard freezer boxes are coated to resist moisture ingress and are optimized for cost-efficient high-density storage in ultra-low temperature environments.
The 81-place (9&times;9 grid) polycarbonate cryobox remains the standard format in most biorepositories and clinical research facilities due to its compatibility with SBS footprint freezer systems and automated inventory tracking workflows. These systems are widely used in genomics, cell banking, and pharmaceutical research environments.
For product details, specifications, or bulk ordering support, <a href="https://mbpinc.net/contact-us">the MBP team can help you</a> choose the right storage solution for your laboratory needs.

Datsheet

Hexagonal Program Cooling Box

Square Program Cooling Box

Cell Freezing Box

The 200101 by NEST Scientific (FreezerCell) is an isopropanol-free controlled-rate cell freezing container that delivers −1 °C per minute when placed in a −80 °C freezer, using a foam-and-alloy design with no liquid additives. Holds 12 cryovials (1 mL or 2 mL) in a hexagonal format for even cooling distribution.

Ice Free Cool Box for 12 Tubes, 1mL/2mL Tubes, Hexagonal Shape, 1/pk, 1/cs

Ice Free Cool Box for 12 Tubes, 1mL/2mL Tubes, Square Shape, 1/pk, 1/cs

Cell Scrapers

<h2 dir="ltr"> What are Cell Scrapers?</h2>
 Cell scrapers are sterile, single-use disposable tools with a polystyrene handle and a free-rotating polyethylene blade used to mechanically detach and harvest adherent mammalian cells from the TC-treated surface of culture flasks, dishes, and multi-well plates when enzymatic detachment (trypsin, Accutase, Dispase) is not appropriate. The free-rotating blade mechanism allows the angle to be adjusted by applying gentle pressure on the handle &mdash; the blade twists to align with the vessel surface for consistent, complete cell removal across the full growth area. Choose the 13 mm blade for T25 and T75 flasks and multi-well plates; choose the 20 mm blade for T175, T225, and 100&ndash;150 mm dishes, where a wider blade reduces the number of passes needed to clear the full growth surface.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Cell Scrapers with 18cm Handle for compact culture vessels</li>
<li dir="ltr" role="presentation">Cell Scrapers with 24.3cm Handle for extended reach in flasks</li>
<li dir="ltr" role="presentation">220mm Handle Scrapers with 13mm Blade for precision harvesting</li>
<li dir="ltr" role="presentation">280mm Handle Scrapers with 20mm Blade for large-surface area recovery</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Scrapers</h2>
 When to Use Cell Scrapers Instead of Trypsin Cell scraping is the correct harvesting method when:
<ol>
<li style="text-align: left;">Trypsinization damages the protein or surface marker of interest; scraping preserves surface antigens, including CD markers for flow cytometry and receptor proteins for biochemical assays.&nbsp;</li>
<li style="text-align: left;">The cell line does not detach effectively with trypsin (some primary cells, fibroblasts in dense monolayers, epithelial cells after extended culture)</li>
<li style="text-align: left;">Harvest must be complete and rapid for protein or RNA extraction protocols with RNase-sensitive targets, where trypsin incubation time introduces RNA degradation risk</li>
<li style="text-align: left;">The downstream assay uses cells in medium without trypsin carryover (some enzyme assays, reporter gene assays, and cell viability assays where trypsin activity in the sample affects results). </li>
</ol>
Blade Size by Vessel Format The 13 mm blade (catalog 710001, 220 mm handle) is designed for T25 and T75 flasks and multi-well plates (6-well through 24-well). The blade width covers approximately 1&ndash;1.5 passes across a T75 flask width for complete surface coverage. The 20 mm blade (catalog 710011, 280 mm handle) suits T175 and T225 flasks, 100 mm and 150 mm dishes, and any large-surface vessel where the wider blade reduces scraping passes needed and operator fatigue. Both blade sizes fit through the standard T-flask neck opening. Free-Rotating Blade Mechanism NEST Scientific cell scrapers use a free-rotating blade that pivots relative to the handle on a hinge mechanism. Applying gentle lateral pressure on the handle adjusts the blade angle, allowing it to maintain flush contact with the vessel floor during the scraping stroke &mdash; improving cell detachment yield compared to fixed-blade scrapers, where the blade angle may not match the vessel floor geometry. After the push stroke, rotating the handle slightly returns the blade to the correct alignment for the next stroke. Scraping Technique Add 1&ndash;2 mL of culture medium or PBS to the vessel before scraping to lubricate the surface and prevent cells from drying. Work systematically across the flask or dish in parallel overlapping strokes. For T-flasks, tilt the flask at 20&ndash;30&deg; to pool the cell suspension at the low end after scraping, then pipette out the suspension. Do not press excessively &mdash; scraping force sufficient to detach cells should not require grinding the blade into the vessel floor, which damages cells and the scraper. 
<h2 style="text-align: left;"> Specifications Context</h2>
 NEST Scientific cell scrapers (catalog 710001 and 710011) are manufactured with a polystyrene handle and polyethylene blade. E-beam sterilized per ANSI/AAMI/ISO 11137 to SAL 10⁻⁶; individually wrapped in easy-tear sterile packaging; non-pyrogenic, DNase/RNase-free per lot certificate. Performance testing is conducted on each manufacturing lot per NEST Scientific standard operating procedures. NEST Scientific cell scrapers are available through MBP in cases of 100 (1/pk &times; 100/cs). The free-rotating blade design is a confirmed feature per NEST Scientific USA product documentation. 
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<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the team at Molecular Biology Products Inc.</a> to request a quote for your laboratory supply needs.

Cell Scraper Specifications

Cell Scraper sterile, individually wrapped, 1/pk, 100/cs

NEST Scientific 710001 cell scraper: 220 mm handle, 13 mm adjustable-angle blade, sterile individually wrapped. Designed for mechanical adherent cell detachment from T-25 and T-75 flasks without enzymatic dissociation. 100 per case. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

Cell scraper, handle 220mm, blade 13mm, sterile, individually wrapped, 1/pk, 100/cs

Cell scraper, handle 280mm, blade 20mm, sterile, individually wrapped, 1/pk, 100/cs

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

MSDS

Cell-free Recovery Buffer (20 ml)

Cell-free Recovery Buffer (R1072-3-20) by Zymo Research — 20 ml replacement component for the Quick-cfDNA/cfRNA Serum &amp; Plasma Kit. Separates RNA from DNA into separate final eluates. Room temperature storage.

Shipping Condition

Storage Condition

Centrifuge Bottles

<h2 data-section-id="1x7fdl3" data-start="0" data-end="31">What Are Centrifuge Bottles?</h2>
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Centrifuge bottles are large-volume vessels made from polypropylene copolymer (PPCO) or polypropylene (PP), designed for low- to moderate-speed centrifugation of biological and chemical samples. Available in volumes ranging from 250 mL to 1,000 mL, these bottles feature a bottle-shaped body with either a flat or rounded bottom and are designed to fit swing-bucket and fixed-angle rotors used in preparative centrifuges and floor-standing centrifuge systems.
They are commonly used for large-scale mammalian cell culture harvesting, ammonium sulfate protein precipitation, bacterial cell pelleting, density-gradient separations, and biopharmaceutical process clarification where standard centrifuge tubes do not provide sufficient capacity.
Use centrifuge bottles when processing volumes exceed 250 mL per run and your centrifuge rotor is designed for bottle-format vessels. Always verify fill requirements and rotor compatibility before use.
&nbsp;
<h2 dir="ltr">In this section:</h2>
&nbsp;
250mL Centrifuge Bottles &ndash; The perfect "middle-weight" option for pilot-scale prep and mid-range harvesting.
500mL Centrifuge Bottles &ndash; Our high-capacity workhorses for bulk concentration and large-scale cell separations.
&nbsp;
<h2 data-section-id="152gqj6" data-start="991" data-end="1026">How to Choose Centrifuge Bottles</h2>
&nbsp;
Material: PPCO vs PP
Polypropylene copolymer (PPCO) centrifuge bottles offer enhanced flexibility and impact resistance compared to standard polypropylene. This helps reduce the risk of cracking during repeated autoclaving cycles or accidental handling impacts. PPCO bottles are translucent, allowing users to visually monitor samples without removing bottles from the rotor.
Standard polypropylene bottles provide similar chemical resistance but are generally more rigid. Selection should be based on the application's mechanical and chemical requirements.
Volume Selection: 250 mL, 500 mL, and 1,000 mL
250 mL centrifuge bottles are typically used for process volumes between 200 and 225 mL and are suitable for medium-scale laboratory workflows.
500 mL centrifuge bottles accommodate process volumes of approximately 400&ndash;450 mL and are commonly used for larger culture harvests and clarification procedures.
1,000 mL centrifuge bottles are designed for high-volume applications including bacterial fermentation harvesting, large-scale density-gradient preparation, and manufacturing-scale clarification processes. Because of their larger size, these bottles generally have lower maximum centrifugal force ratings than smaller formats.
Cap Type: Standard Screw Cap vs Sealing Cap
Standard polypropylene screw caps provide reliable leak protection during routine centrifugation and storage applications.
Sealing cap assemblies incorporate additional gasket-based sealing systems that improve containment during higher-speed centrifugation and when handling biohazardous materials. These sealing systems are commonly used when aerosol containment or enhanced sample security is required.
Rotor and Adapter Compatibility
Centrifuge bottles must be matched to compatible rotor systems and adapters. Bottle dimensions, neck designs, and rotor requirements vary by manufacturer and volume. Before purchasing, laboratories should verify compatibility between the bottle, rotor, and any required adapters to ensure safe operation.
Fill Level Requirements
Proper fill volume is critical for safe centrifugation. Most centrifuge bottles should be filled to at least 80% of their total capacity to prevent excessive stress on the bottle walls during centrifugation. Underfilled bottles may deform or collapse under centrifugal force. For some larger formats, maximum fill recommendations may also apply and should be verified with manufacturer specifications.
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<h2 data-section-id="1mxgh1c" data-start="3525" data-end="3561">Compare Centrifuge Bottle Formats</h2>
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250 mL PPCO centrifuge bottles are manufactured from polypropylene copolymer and are typically supplied in packs of four bottles per case. When used with appropriate sealing cap assemblies, they can support significantly higher centrifugal forces than standard-cap configurations.
500 mL PPCO centrifuge bottles provide increased sample capacity for larger-volume processing applications. These bottles are commonly supplied with standard screw caps and are packaged in case quantities of four bottles.
1,000 mL PPCO centrifuge bottles are intended for large-scale harvesting and clarification workflows. Although they provide the highest volume capacity, they generally operate at lower maximum centrifugal force ratings than smaller bottle formats. Packaging typically consists of four bottles per case.
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<h2 data-section-id="1jl751w" data-start="4384" data-end="4409">Specifications Context</h2>
&nbsp;
PPCO centrifuge bottles are manufactured from durable polypropylene copolymer materials that offer excellent chemical resistance, translucency for sample visibility, and compatibility with autoclave sterilization procedures. They are widely used with preparative centrifuge systems, including various fixed-angle and swing-bucket rotor configurations.
As of 2026, MBP supplies centrifuge bottle formats suitable for preparative centrifugation applications. Available volumes, cap assemblies, and compatibility options may vary depending on product line and manufacturer specifications.
Let's make sure your lab has the exact precision tools your research requires. Get a quote from the <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Molecular Biology Products Inc. team</a> today.

6330-6332 by Globe Scientific are polypropylene centrifuge bottles in 250 mL (SKU 6330, rated to 27,500 × g) and 500 mL (SKU 6332, rated to 4,800 × g) sizes, at 80% fill. Linerless leakproof closure, autoclavable at 121°C, reusable, with excellent chemical resistance. Supplied 4 per bag for low-to-moderate speed centrifugation applications.

Manufacture

Centrifuge Bottle, 250mL, Attached Cap, PP, 4/Bag, CS/36

Centrifuge Bottle, 500mL, Attached Cap, PP, 4/Bag, CS/24

Centrifuge Tubes

<h2>What Are Centrifuge Tubes?</h2>
 Centrifuge tubes are cylindrical laboratory containers with caps that are used to separate, pellet, and store biological or chemical samples through centrifugal force. They are commonly made from polypropylene (PP) or polystyrene (PS) and are available in sizes ranging from small 0.5 mL microtubes to larger 50 mL conical tubes. These tubes are widely used for cell pelleting, protein precipitation, DNA and RNA extraction, tissue homogenate clarification, and long-term sample storage. Depending on the material, they can withstand temperatures from &minus;80 &deg;C to +121 &deg;C.
In short, the best centrifuge tube depends on your centrifuge rotor, required g-force, sample volume, and sterility needs. 
<h2>What You Will Find: </h2>
<ul>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes" title="All Centrifuge Tubes">All Centrifuge Tubes</a>: Comprehensive selection of 15 mL and 50 mL conical tubes with clear graduations for daily lab workflows.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/oak-ridge-style-centrifuge-tubes" title="Oak Ridge Style Centrifuge Tubes">Oak Ridge Style Centrifuge Tubes</a>: High-speed, chemical-resistant tubes designed to withstand extreme RCF without deformation.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/centrifuge-bottles" title="Centrifuge Bottles">Centrifuge Bottles</a>: Heavy-duty, larger-capacity vessels engineered for bulk sample processing and high-velocity runs.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes" title="Large Volume Centrifuge Tubes">Large Volume Centrifuge Tubes</a>: Industrial-strength 250 mL and 500 mL tubes optimized for pilot-scale harvesting and bioprocessing.</li>
</ul>
&nbsp;
<h2>How to Choose Centrifuge Tubes</h2>
 Volume and Rotor Compatibility
Select a tube size that matches both your sample volume and centrifuge rotor. Microtubes ranging from 0.5&ndash;2 mL are commonly used in microcentrifuges and can typically handle speeds up to 20,000 &times; g. Midi tubes in the 5&ndash;10 mL range are suitable for intermediate sample volumes, while 15 mL and 50 mL conical tubes are commonly used in clinical, research, and preparative centrifuges.
Material: Polypropylene vs. Polystyrene
Polypropylene (PP) tubes offer excellent chemical resistance and can tolerate temperatures from &minus;80 &deg;C to +121 &deg;C. They are suitable for autoclaving and exposure to many laboratory solvents. Polystyrene (PS) tubes provide superior optical clarity, making it easier to visually inspect samples and liquid levels, but they have lower resistance to solvents and extreme temperatures.
Sterility and Contamination Control
Sterile centrifuge tubes are typically gamma- or E-beam-irradiated and certified free of RNase, DNase, and pyrogens. These tubes are recommended for RNA extraction, cell culture, and clinical applications where contamination must be minimized. Non-sterile tubes may still be RNase- and DNase-free and are often suitable for routine DNA work and general sample storage.
Cap Design
Attached screw caps help prevent cap mix-ups and sample contamination, especially in high-throughput laboratories. Separate caps can be quicker to open and may be preferred in workflows that require frequent access to samples. If tubes will be used with automated systems, cap dimensions should be verified for compatibility with liquid handling equipment.
Packaging Options
Racked tubes provide convenient organization and allow direct pipetting while the tubes remain in the rack. Bulk-bagged tubes offer a more economical option for laboratories that process large volumes of samples and already have tube storage systems in place.
<h2> Comparing Common Centrifuge Tube Types</h2>
 Microtubes (0.5&ndash;2 mL) are ideal for PCR preparation, microcentrifuge applications, and storage of small sample volumes. They can generally withstand centrifugal forces up to 20,000 &times; g.
Midi tubes (5&ndash;10 mL) are designed for intermediate sample volumes such as culture supernatants and can typically tolerate forces up to 16,000 &times; g.
Sterile 15 mL conical tubes are frequently used for cell pelleting, RNA extraction, and clinical sample processing, with typical ratings between 12,000 and 15,000 &times; g.
Sterile 50 mL conical tubes are commonly selected for larger-volume applications including tissue culture work, density gradient preparation, and large-scale sample processing.
Polystyrene conical tubes in 15 mL and 50 mL formats are often chosen when visual sample inspection is important and solvent exposure is minimal.
<h2> Specifications and Features</h2>
 Polypropylene centrifuge tubes from Globe Scientific and Nest Scientific are manufactured from USP Class VI virgin resin and do not contain plasticizers or processing additives. Sterile products are typically irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶. Most 15 mL tubes feature graduation marks at 1 mL intervals, while 50 mL tubes are usually graduated every 5 mL. MBP offers both bagged and racked centrifuge tube formats, including 15 mL and 50 mL conical tubes as well as smaller microtube and midi tube options.
MBP supports procurement departments by accepting purchase orders and offering institutional pricing for qualified high-volume customers. <a href="https://mbpinc.net/contact-us">Contact the MBP team today</a> for a customized quote.

Centrifuge Tube

Globe Scientific 112009 Quick-Prep™ Urine Centrifuge Tubes are 12 mL polystyrene conical tubes (16 × 100 mm) designed for use with Bayer® urine analyzers, with graduations at 0.5–10 mL and a maximum speed of 1,500 RPM. Supplied non-sterile with unattached white plug caps, case of 1,000.

Centrifuge Tube, 16 x 100mm (12mL), PS, with Separate White Plug Cap, CS/1000

Centrifuge tube, 12mL, Molded Graduations, 16.5 x 107.5mm, PS, 100/Bag, 10 Bags/Case

<h2 data-section-id="155qizo" data-start="0" data-end="33">What Are All Centrifuge Tubes?</h2>
&nbsp;
The All Centrifuge Tubes collection includes the complete range of tube sizes commonly used in research, clinical, and laboratory environments. These tubes range from small 0.5 mL and 1.5&ndash;2 mL microtubes used in microcentrifuge applications to 5 mL and 10 mL midi tubes for intermediate sample volumes, as well as 15 mL and 50 mL conical tubes designed for cell culture, density gradient preparation, and larger-volume sample processing. They are available in polypropylene (PP) for enhanced chemical resistance and temperature tolerance or polystyrene (PS) for improved optical clarity.
&nbsp;
What you will find:
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microcentrifuge-tubes" title="Microcentrifuge Tubes">Microcentrifuge Tubes</a> &ndash; Precision-engineered for small-volume sample preparation.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/screw-cap-tubes" title="Screw Cap Tubes">Screw Cap Tubes</a> &ndash; Secure, leak-proof closures for long-term storage and transport.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/15ml-and-50ml-centrifuge-tubes" title="15ml &amp; 50ml Centrifuge Tubes">15ml &amp; 50ml Centrifuge Tubes</a> &ndash; Standard conical bottom tubes for large-volume processing.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/midicentrifuge-tubes-5ml-and-10ml" title="Midicentrifuge Tubes (5ml &amp; 10ml)">Midicentrifuge Tubes (5ml &amp; 10ml)</a> &ndash; The perfect bridge for intermediate sample sizes.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microtubes-box" title="Microtubes Box">Microtubes Box</a> &ndash; Durable storage racks for organized sample management and protection.
</li>
</ul>
&nbsp;
<h2 data-section-id="1f93yys" data-start="914" data-end="963">How to Choose From All Centrifuge Tube Formats</h2>
&nbsp;
Volume and Rotor Compatibility
Selecting the correct tube size is essential for safe and efficient centrifugation. Microtubes ranging from 0.5&ndash;2 mL are designed for microcentrifuges, while 5&ndash;10 mL midi tubes fit compatible benchtop centrifuge rotors. Larger 15 mL and 50 mL conical tubes are commonly used in clinical and research centrifuges. Always verify rotor compatibility before introducing a new tube format into your workflow.
Sterility Requirements
For sensitive applications such as RNA extraction, cell culture, and clinical diagnostics, sterile tubes certified free of RNase, DNase, and pyrogens are recommended. Non-sterile tubes can provide a cost-effective solution for routine DNA analysis, protein assays, and general sample storage where sterility is not critical.
Material Selection
Polypropylene tubes offer excellent durability, can be autoclaved at 121 &deg;C, and resist many laboratory chemicals and solvents. Polystyrene tubes provide superior transparency, making it easier to monitor liquid levels and sample contents, although they are less resistant to heat and solvents.
Packaging and Inventory Planning
Microtubes are commonly available in pack sizes ranging from 500 to 1,000 units, while 15 mL and 50 mL conical tubes are often supplied in cases of 500. Laboratories with consistent usage may benefit from establishing recurring purchase orders to ensure uninterrupted supply.
&nbsp;
<h2 data-section-id="urfo3f" data-start="2395" data-end="2433">Compare Centrifuge Tube Size Groups</h2>
&nbsp;
Microtubes (0.5&ndash;2 mL) are primarily used for PCR preparation, microcentrifuge applications, and storage of small sample volumes. These are typically manufactured from polypropylene and are available in pack sizes of 500&ndash;1,000 units.
Midi tubes (5&ndash;10 mL) support intermediate-volume centrifugation and supernatant collection. They are commonly made from polypropylene and supplied in packs ranging from 100&ndash;500 units.
15 mL conical tubes are widely used for cell pelleting, RNA isolation, and clinical sample processing. They are available in both polypropylene and polystyrene formats and are generally packaged in cases of 500.
50 mL conical tubes are designed for larger-volume workflows, including tissue culture, density gradient preparation, and sample processing. Like 15 mL tubes, they are available in polypropylene and polystyrene and are commonly sold in cases of 500.
&nbsp;
<h2 data-section-id="1ezckh3" data-start="3318" data-end="3348">Specifications and Features</h2>
&nbsp;
Globe Scientific and Nest Scientific centrifuge tubes are manufactured from USP Class VI virgin polypropylene or polystyrene resin and are certified free from detectable RNase, DNase, and pyrogens. Sterile products are irradiated using gamma or E-beam methods to achieve a sterility assurance level (SAL) of 10⁻⁶. MBP offers both racked and bagged formats for 15 mL and 50 mL conical tubes, along with dispenser-box options for selected microtube product lines that support efficient use in high-throughput laboratory environments.
For procurement teams, MBP accepts purchase orders and offers institutional pricing for bulk and high-volume purchases. <a href="https://mbpinc.net/contact-ushttps://mbpinc.net/contact-us" title="Contact the MBP team" style="color: #236fa1;">Contact the MBP team</a> today to learn more

All Centrifuge Tubes

<h2 data-section-id="hcowtr" data-start="0" data-end="45">What Are 15 mL and 50 mL Centrifuge Tubes?</h2>
&nbsp;
15 mL and 50 mL centrifuge tubes are conical-bottom vessels in polypropylene (PP) or polystyrene (PS), the two most commonly used tube formats in research and clinical laboratories worldwide. The 15 mL tube is the standard for cell pelleting, RNA isolation, small-scale protein purification, and clinical sample preparation. The 50 mL tube handles larger culture volumes, gradient preparation, tissue homogenate clarification, and bulk precipitation steps. Both formats from Globe Scientific and Nest Scientific are rated to 12,000&ndash;15,000 &times; g in a supported rotor.
15 mL for cell and molecular workflows under 12 mL; 50 mL for culture volumes, gradients, and any prep where a 15 mL conical runs out of headroom.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Attached flat top screw cap: Leak-proof, one-hand use</li>
<li>Racked: Pre-organized for high-throughput</li>
<li>Self-standing: Stable skirted base</li>
<li>Sterile: For contamination-sensitive work</li>
<li>Non-sterile: General, cost-effective use</li>
<li dir="ltr" role="presentation">Bulk: High-volume, economical</li>
<li dir="ltr" role="presentation">Racked: Organized &amp; ready-to-use</li>
<li dir="ltr" role="presentation">PS racked: Extra stability with polystyrene racks</li>
</ul>
&nbsp;

<h2 data-section-id="1j0vxh6" data-start="772" data-end="821">How to Choose 15 mL and 50 mL Centrifuge Tubes</h2>
&nbsp;
Material: PP vs PS
Polypropylene tubes (Globe Scientific, Nest Scientific) are resistant to most organic solvents, stable from &minus;80 &deg;C to +121 &deg;C, and autoclavable. Polystyrene tubes offer greater optical clarity &mdash; the tube wall is transparent enough to read the sample meniscus and assess pellet quality without removing the tube from the rack. Choose PS when visual inspection is a workflow step; choose PP for all other applications.
Sterility Grade
Sterile tubes (gamma- or E-beam-irradiated, RNase-free, DNase-free, non-pyrogenic) are required for RNA work, primary cell culture, and clinical sample handling. Non-sterile, DNase/RNase-free tubes are appropriate and more cost-effective for DNA extractions, protein assays, and general preparative spins.
Cap Style: Flat-Top vs Plug-Seal
Flat-top screw caps (high-density polyethylene, HDPE) provide a flat writing surface for sample labeling and a double-threaded design that reduces cross-threading in high-throughput workflows. Plug-seal screw caps (HDPE or PP) create a secondary internal seal for leak-prone applications. Attached caps come pre-assembled on sterile tubes; separate caps are standard on non-sterile tubes.
Pack Format: Racked vs Bagged
Racked tubes (foam or PS rack, 50 &times; 15 mL or 25 &times; 50 mL per rack, 500 per case) allow direct pipetting without removing tubes and enable viewing of graduations without disturbing the rack. Bagged tubes (25 or 50 per bag, 500 per case) offer a lower cost per tube and suit labs with robotic liquid handlers or in-house racking systems.
&nbsp;
<h2 data-section-id="up19i6" data-start="2392" data-end="2431">Compare 15 mL and 50 mL Tube Options</h2>
&nbsp;
Globe Scientific 15 mL polypropylene tubes with flat-top blue caps are available in both sterile and non-sterile formats with attached or separate caps. These tubes can be purchased in racked or bagged packaging configurations, with 500 tubes per case, and are rated to withstand centrifugal forces up to 15,000 &times; g.
Globe Scientific 15 mL polystyrene tubes with red caps provide enhanced optical clarity for visual sample inspection and are available in sterile and non-sterile versions. They are supplied with attached or separate caps, offered in racked or bagged formats, and rated up to 12,000 &times; g.
Globe Scientific 50 mL polypropylene tubes with flat-top blue caps are designed for larger-volume centrifugation workflows. Available in sterile and non-sterile configurations with attached or separate caps, these tubes are supplied in racked or bagged packaging and can withstand centrifugal forces up to 15,000 &times; g.
Globe Scientific 50 mL polystyrene tubes with red caps combine large-volume capacity with excellent transparency for easy sample visualization. They are available in sterile and non-sterile formats, supplied with attached or separate caps, packaged either racked or bagged, and rated up to 12,000 &times; g.
Nest Scientific 15 mL polypropylene tubes are supplied sterile with attached caps and packaged in polystyrene racks containing 500 tubes per case. These tubes are rated to 12,000 &times; g and are commonly used in cell culture, clinical, and molecular biology applications.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3950" data-end="3975">Specifications Context</h2>
&nbsp;
Globe Scientific 15 mL and 50 mL centrifuge tubes are molded from USP Class VI virgin polypropylene or polystyrene, certified RNase-free, DNase-free, and non-pyrogenic by lot. The 15 mL PP tube is rated to 15,000 &times; g; 50 mL PP tubes to 15,000 &times; g in a supported rotor. Graduated markings on 15 mL tubes read every 1 mL (with sub-milliliter graduations below 1 mL); 50 mL tubes every 5 mL. Globe Scientific is ISO 9001:2015 certified. As of 2026, MBP stocks flat-top (blue cap) and plug-seal (red cap) variants in PP and PS, with sterile and non-sterile options available.
Ensure your high-speed separations remain consistent and safe by choosing tubes built for extreme RCF ratings. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the Molecular Biology Products Inc. team</a> for a quote.

15ml & 50ml Centrifuge Tubes

<h2 data-section-id="kb9bww" data-start="0" data-end="42">What Are Large Volume Centrifuge Tubes?</h2>
&nbsp;
Large volume centrifuge tubes are polypropylene conical-bottom vessels available in 250 mL and 500 mL formats, designed for preparative and process-scale centrifugation at speeds of up to 6,000&ndash;7,500 &times; g in compatible swing-bucket or fixed-angle rotors. These tubes are used for applications such as cell harvesting from bioreactors, viral vector and vaccine production, large-scale protein precipitation, gradient preparation, and clarification of culture supernatants where 50 mL tubes are insufficient.
Polypropylene construction provides resistance to most organic solvents, stability across a temperature range of &minus;80 &deg;C to +120 &deg;C, and full autoclavability for sterile processing workflows.
Use 250 mL or 500 mL centrifuge tubes for process-scale volumes above 50 mL when compatible large-capacity rotors and support systems are available.
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<h2 dir="ltr">This section contains:</h2>
&nbsp;
<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/250ml-centrifuge-tubes" title="250mL Centrifuge Tubes">250mL Centrifuge Tubes </a>&ndash; Optimized for pilot-scale harvesting and efficient mid-range sample concentration.
<a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes/500ml-centrifuge-tubes" title="500mL Centrifuge Tubes">500mL Centrifuge Tubes</a> &ndash; Industrial-strength vessels designed for bulk bioprocessing and high-capacity separations.
&nbsp;
<h2 data-section-id="1riycx1" data-start="904" data-end="950">How to Choose Large Volume Centrifuge Tubes</h2>
&nbsp;
250 mL vs 500 mL
Choose 250 mL tubes when process volumes per tube run 150&ndash;240 mL &mdash; overfilling beyond ~80% of rated volume risks spill and cap failure. 500 mL tubes are used for volumes from 300&ndash;480 mL, common in vaccine and viral vector production, large-scale mammalian cell culture harvest, and preparative density-gradient centrifugation. The 500 mL tube has a larger diameter (95 mm) and shorter height (146 mm) than the 250 mL tube (60 mm &times; 160 mm), requiring a different rotor adapter.
Support Cushions and Rotor Compatibility
Large volume centrifuge tubes require properly fitted rotor support cushions (typically made from polyetherimide or similar materials) unless the rotor is specifically designed for direct tube support. These cushions prevent tube wall deformation under centrifugal force. Without correct support, tube failure may occur. Compatibility between tubes, cushions, and rotor buckets must always be confirmed before use.
Cap Type: Plug-Seal vs Flat Screw Cap
Plug-seal screw caps made from high-density polyethylene (HDPE) provide an internal sealing mechanism that improves leak resistance during high-force centrifugation, especially with viscous or biohazardous materials. Flat screw caps are generally used for lower-risk applications involving mixing or storage. Large volume polypropylene tubes are typically supplied with plug-seal caps rated for reliable containment under pressure conditions.
Sterility and Certification
Sterile large volume centrifuge tubes are gamma-irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶ and are required for applications such as viral vector production, vaccine manufacturing, and primary cell culture workflows. Non-sterile RNase-free and DNase-free tubes are suitable for chemical processing steps and downstream analytical procedures where sterility is not critical.
&nbsp;
<h2 data-section-id="1xe8cvr" data-start="3019" data-end="3066">Compare Large Volume Centrifuge Tube Formats</h2>
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250 mL polypropylene conical centrifuge tubes are designed for intermediate process-scale applications. These tubes typically measure around 60 mm in diameter and 160 mm in height and are rated for centrifugal forces up to approximately 6,000&ndash;7,500 &times; g. They are commonly supplied sterile with HDPE plug-seal caps and are packaged in bags, often in case quantities of around 70 tubes per case.
500 mL polypropylene conical centrifuge tubes are intended for large-scale processing applications involving high culture volumes or bulk precipitation workflows. These tubes typically measure around 95 mm in diameter and 146 mm in height and are rated up to approximately 6,000 &times; g. They are supplied sterile with HDPE plug-seal caps and are commonly packaged in case quantities of around 40 tubes per case.
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<h2 data-section-id="1jl751w" data-start="3881" data-end="3906">Specifications Context</h2>
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Large volume polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified sterile, RNase-free, DNase-free, and pyrogen-free. Sterile lots are typically gamma-irradiated to SAL 10⁻⁶ in accordance with ISO 11137 standards. Molded graduations allow approximate volume estimation without removing tubes from the centrifuge rotor.
As of 2026, MBP stocks both 250 mL and 500 mL large volume centrifuge tube formats. Support cushions and rotor-specific accessories are typically required and must be verified for compatibility prior to ordering.
Ready to secure your high-volume runs? <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Reach out to the Molecular Biology Products Inc. team</a> for a custom quote today.

Large Volume Centrifuge Tubes

<h2>What Are 250 mL Centrifuge Tubes?</h2>
&nbsp;
250 mL centrifuge tubes are large-volume polypropylene conical tubes designed for processing sample volumes that exceed the capacity of standard 50 mL conical tubes. They are commonly used for cell culture harvests, density-gradient separations, protein precipitation, viral workflows, and other preparative laboratory applications.
These tubes provide an efficient solution for handling medium- to large-scale samples while remaining easier to manage than 500 mL formats. Most models feature leak-resistant caps, molded graduations, and compatibility with swing-bucket centrifuge rotors designed for large-volume processing.
&nbsp;
<h2>What we offer at MBP:</h2>
&nbsp;
<ul>
<li>250 mL centrifuge tubes with leak-proof caps and molded graduations</li>
<li>Tubes with transfer-cap configurations for closed-system fluid handling</li>
<li>Gamma-irradiated sterile tubes for biopharmaceutical and cell culture applications</li>
<li>Autoclavable polypropylene vessels for repeated sterilization cycles</li>
<li>RNase-free and DNase-free tubes for molecular biology workflows</li>
<li>Large-volume centrifuge tubes designed for demanding processing applications</li>
</ul>
&nbsp;
<h2>How to Choose 250 mL Centrifuge Tubes</h2>
&nbsp;
Rotor Compatibility Verify that your centrifuge system supports 250 mL tubes and any required adapters or support cushions. Proper rotor compatibility is essential for safe and reliable operation during centrifugation.
Sterility Requirements Sterile, gamma-irradiated tubes are recommended for cell culture, viral, and sensitive biological applications. Non-sterile options are suitable for general laboratory processing and chemical workflows where sterility is not required.
Graduations and Visibility Molded graduations provide convenient approximate volume measurements and should remain visible after repeated use and sterilization procedures.
Packaging and Supply Planning Large-volume centrifuge tubes are often supplied in moderate case quantities. Laboratories with frequent processing requirements may benefit from maintaining regular inventory levels to avoid workflow interruptions.
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<h2>Specifications Context</h2>
&nbsp;
250 mL centrifuge tubes are typically manufactured from high-clarity polypropylene and fitted with secure, leak-resistant caps for safe sample handling. Many products are autoclavable and suitable for temperature ranges commonly encountered in laboratory storage and sterilization procedures.
Sterile versions are available with certifications such as RNase-free, DNase-free, and non-pyrogenic status to support molecular biology and bioprocessing applications. Proper accessories, including compatible rotor cushions and adapters, should always be verified before use.
As of 2026, MBP offers large-volume centrifuge tubes for research, clinical, and bioprocessing laboratories. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for product information and technical support.

250ml Centrifuge Tubes

<h2 data-section-id="57m9pt" data-start="0" data-end="36">What Are 500 mL Centrifuge Tubes?</h2>
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500 mL centrifuge tubes are large-format polypropylene conical vessels used for process-scale centrifugation applications where sample volumes exceed approximately 300 mL per tube. These tubes typically have standard dimensions of 95 mm outer diameter and 146 mm height, and feature a conical bottom that enables efficient pellet formation and recovery. They are rated for use up to 6,000 &times; g in a compatible swing-bucket rotor system equipped with polyetherimide (PEI) support cushions.
These tubes are commonly used in large-scale mammalian cell culture harvesting from bioreactors, viral vector production (including AAV and lentivirus), vaccine manufacturing workflows, large-volume density-gradient separations, and preparative-scale protein or nucleic acid precipitation.
500 mL centrifuge tubes are used when process volumes are 300&ndash;480 mL per run and a 250 mL tube would require multiple centrifugation cycles.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">The MBP Quality Standard:</h2>
&nbsp;
<ul>
<li dir="ltr">High-capacity 500ml vessels for industrial-strength bulk concentration.</li>
<li dir="ltr">Bi-directional transfer caps with silicone tubing for closed-system fluid handling.</li>
<li dir="ltr">Gamma-irradiated sterile containers validated for aseptic processing.</li>
<li dir="ltr">Chemical-resistant construction for durability against aggressive buffers.</li>
</ul>
&nbsp;
<h2 data-section-id="1njhdwk" data-start="971" data-end="1011">How to Choose 500 mL Centrifuge Tubes</h2>
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Rotor Compatibility First
500 mL centrifuge tubes require a compatible swing-bucket rotor system that can accommodate a 95 mm tube diameter along with appropriate PEI support cushions. Common compatible systems include Thermo Fisher TX-750 and equivalent swing-bucket rotor models. It is essential to confirm both rotor bucket compatibility and correct cushion part numbers before use.
Using 500 mL tubes without proper support cushions or in incompatible rotors can lead to tube deformation or failure even at relatively low centrifugal forces.
Sterility for Bioproduction Workflows
Sterile 500 mL centrifuge tubes are gamma-irradiated to a sterility assurance level (SAL) of 10⁻⁶ and are certified RNase-free, DNase-free, and non-pyrogenic. These are required for bioproduction applications such as viral vector manufacturing, vaccine production, and primary cell culture workflows.
Non-sterile tubes may be used for downstream processing steps, chemical precipitation, or other laboratory applications where sterility is not required.
Cap Integrity and Leak Prevention
500 mL tubes are equipped with HDPE plug-seal screw caps that create an internal compression seal within the tube neck. This design provides leak resistance up to 95 kPa (14 psi), which is important for handling biohazardous or high-risk samples such as BSL-2 cell lines or viral vectors.
Proper cap closure is critical before centrifugation; caps must be fully tightened without cross-threading to ensure safe operation.
Pack Size and Ordering Considerations
These tubes are typically supplied in packs of 5 tubes per bag and 8 bags per case, totaling 40 tubes per case. Because of the relatively low number of tubes per case compared to smaller formats, laboratories performing high-throughput bioprocessing often maintain standing purchase orders to avoid supply interruptions.
&nbsp;
<h2 data-section-id="1hsndwv" data-start="2893" data-end="2933">Specifications Context</h2>
&nbsp;
500 mL polypropylene centrifuge tubes are manufactured from high-clarity polypropylene resin and are certified RNase-free, DNase-free, and non-pyrogenic. Sterilization is performed via gamma irradiation in compliance with ISO 11137 standards, achieving a sterility assurance level of 10⁻⁶. Molded graduations on the tube body allow approximate volume measurement without removing the tube from the centrifuge rotor.
As of 2026, MBP supplies 500 mL large volume centrifuge tubes; availability, brand options, and lot-specific details may vary. Support cushions are required for safe operation and must be confirmed as compatible with the selected rotor system prior to ordering.
Secure your research and your results. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> for a quote.

500ml Centrifuge Tubes

Centrifuge Tube Closed System Solution

Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube

C60622-EFB050A by NEST Scientific — 50 mL centrifuge tube with bi-directional transfer cap, silicone tube (1/8&quot; ID &times; 1/4&quot; OD), male Luer Lock connector, 0.22 &mu;m &times; 24 mm vent filter. Sterile, 1/pk, 4/cs.

50mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

250mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

500mL Centrifuge Tube with Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

Universal Centrifuge Accessories

<h2>What Are Universal Centrifuge Accessories?</h2>
&nbsp;
Universal centrifuge accessories are the interchangeable components that determine the range of applications a universal centrifuge can perform. These include swing-out rotor buckets for blood tubes, conical tubes, microplates, and large bottles; fixed-angle rotors for high-speed pelleting; hematocrit and cytospin rotors; tube adapters; and aerosol-tight bio-containment lids. Selecting the appropriate accessories allows a single centrifuge to support multiple laboratory workflows efficiently and safely.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Tube adapters and inserts for compatibility with multi-volume samples&nbsp;
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Fixed-angle and swing-out rotors for varied centrifugation applications
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Lids and sealed buckets for safe and reliable operations
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
PCR adapters and microplate carriers for high-throughput workflows
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Centrifuge tubes, holders, and supporting aids for efficient and complete setups
</li>
</ul>
&nbsp;
<h2>How to Choose Universal Centrifuge Accessories</h2>
&nbsp;
Swing-Out Bucket Selection by Application
Choose swing-out buckets according to the sample formats used in your laboratory. Blood tube buckets support routine clinical applications, conical tube buckets are suitable for cell culture and molecular biology, microplate carriers enable high-throughput workflows, and large-bottle carriers accommodate large-volume sample processing.
Fixed-Angle Rotors
Fixed-angle rotors provide higher centrifugal forces for microtube, PCR tube, and small-volume sample processing. Ensure that both the rotor and centrifuge motor specifications support the required operating speeds and sample formats.
Cytospin Clip Rotors
Cytospin rotors are designed for preparing microscope slides from low-cellularity samples such as body fluids. Verify compatibility with the required funnel sizes and use bio-containment features when processing potentially infectious materials.
Bio-Containment Lids
Aerosol-tight lids help contain biological samples during centrifugation and support safe laboratory practices. Regular inspection of sealing components and O-rings ensures reliable containment and long-term performance.
Reducing Adapter Inserts
Adapter inserts allow smaller tubes to fit securely within larger bucket positions. Using properly matched adapters improves balance, prevents movement during operation, and helps maintain consistent centrifugation results.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Modern universal centrifuge systems support interchangeable accessory configurations that enable rapid transitions between clinical, research, cell culture, and cytology workflows. Swing-out buckets, fixed-angle rotors, and microplate carriers provide flexibility while maintaining compatibility with a broad range of vessel formats.
Routine inspection and maintenance of accessories, including adapters, lids, and sealing components, help ensure safe operation and extend equipment lifespan. Advances in bio-containment systems and modular rotor designs continue to improve laboratory efficiency and versatility.
Time to upgrade your universal centrifuge accessories. <a href="https://mbpinc.net/contact-us">Contact the expert team </a>of Molecular Biology Products Inc. (MBP) and the find perfect match today.

Centrifuge Tube, 175mL, PP, sterile, with screw-cap, 20 bags of 6 tubes, 120/cs

C0175 by MTC Bio is a 175 ml polypropylene centrifuge tube, sterile, RNase/DNase/pyrogen-free, rated to 6,000 × g with screw-cap closure and molded graduations. Supplied 120/cs (20 bags of 6). Standard format for large-volume cell harvest, nucleic acid precipitation, and bulk sample processing.

Centrifuge Tube, 225mL, PP, sterile, with screw-cap, 5 per bag, 17 bags per case, 85/cs

The C0225 by Globe Scientific is a sterile 225ml polypropylene centrifuge tube with an attached HDPE plug-seal screw cap, rated to 7,500 xg, autoclavable at 121°C, and freezable to −40°C. Gamma-irradiated to SAL 10−6, RNase/DNase-free, and non-pyrogenic. Supplied 85 per case (5 per bag, 17 bags).

Centrifuge Tube, 250mL, PP, sterile, with screw-cap, 14 bags of 5 tubes, 70/cs

The C1250 by MTC Bio is a 250 ml PP sterile centrifuge tube with leak-proof screw cap, rated to 6,000 xg. RNase-free, DNase-free, and pyrogen-free with molded graduations 0.5–250 ml. 70 tubes per case (14 bags of 5). Ideal for cell harvesting, protein precipitation, and drug discovery. Available from MBP Inc.