Brands

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green Fast qPCR Mix HiRox

AzuraQuant Green Fast qPCR Mix

MSDS

AzuraQuant™ Green Fast qPCR Mix HiRox

AzuraQuant™ Green Fast qPCR Mix HiRox (AZ-2001) by Azura Genomics is a 2× hot-start SYBR-channel qPCR master mix with a high-ROX passive reference dye, optimised for Applied Biosystems and QuantStudio platforms. Compatible with standard and fast cycling protocols. Available in 100, 500, 2,000, and 5,000 reaction sizes from MBP Inc.

Manufacturer

AzuraQuant Green Fast qPCR Mix HiRox, 100 reactions

AzuraQuant Green Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Green Fast qPCR Mix HiRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix HiRox 5,000 reactions

User Manual

AzuraQuant Green Fast qPCR Mix - Product Sheet

qPCR Reagent Compatibility Tool

AzuraQuant™ Green Fast qPCR Mix LoRox

AZ-2101 by Azura Genomics — AzuraQuant™ Green Fast qPCR Mix LoRox, 2× master mix with Azura HS Taq, SYBR Green channel, low ROX. 20 µL standard reactions, fast-cycling protocol (95°C 2 min / 5 sec denaturation / 20–30 sec anneal-extension). Stored at −20°C, 18-month shelf life. Available in 100–5,000 reaction packs.

AzuraQuant Green Fast qPCR Mix LoRox 100 reactions

AzuraQuant Green Fast qPCR Mix LoRox 500 Reactions

AzuraQuant Green Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix LoRox 5000 reactions

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Product Literature

AzuraQuant™ II cDNA Synthesis Kit

AZ-2501 by Azura Genomics is a 2-tube first-strand cDNA synthesis kit built on HighTherm™ II reverse transcriptase. Converts 4 pg to 2 µg total RNA to cDNA in 25 minutes using a combined random hexamer and anchored oligo(dT) strategy. Available in 25, 100, and 500 reaction formats for 2-step RT-qPCR and RT-PCR workflows.

AzuraQuant II cDNA Synthesis Kit 25 Reactions

AzuraQuant II cDNA Synthesis Kit 100 Reactions

AzuraQuant II cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit - Product Sheet

AzuraQuant™ cDNA Synthesis Kit

AZ-1995 by Azura Genomics is the AzuraQuant™ cDNA Synthesis Kit for 2-step RT-qPCR and RT-PCR. Two-tube format: 5x Buffer and 20x HighTherm™ RT with RNase inhibitor, plus random hexamer and anchored oligo(dT) primers. RNA input from 4 pg. Available in 25, 100, and 500 reaction sizes.

AzuraQuant cDNA Synthesis Kit 25 Reactions

AzuraQuant cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit 100 reactions

AzuraView™ GreenFast qPCR Blue Mix HR

AZ-2401 by Azura Genomics — AzuraView GreenFast qPCR Blue Mix HR; 2X SYBR Green master mix; Azura HS Taq; inert blue tracer dye; increased resistance to freeze-thaw; compatible with all standard qPCR instruments; 100 reactions (1×1 mL).

AzuraView GreenFast qPCR Blue Mix HR 100 Reactions

AzuraView GreenFast qPCR Blue Mix HR 500 Reactions

AzuraView GreenFast qPCR Blue Mix HR 2,000 Reactions

AzuraView GreenFast qPCR Blue Mix HR 5,000 Reactions

AzuraView GreenFast qPCR Blue Mix - Product Sheet

AzuraView™ GreenFast qPCR Blue Mix LR

AzuraView™ GreenFast qPCR Blue Mix LR (AZ-2301) by Azura Genomics — 2× ultra-sensitive intercalating dye master mix with Azura HS Taq, blue tracer dye, low ROX. Standard and fast cycling compatible, stable through 40 freeze-thaw cycles. Available from 100 to 10,000 reactions.

AzuraView GreenFast qPCR Blue Mix LR 100 Reactions

AzuraView GreenFast qPCR Blue Mix LR 500 Reactions

AzuraView GreenFast qPCR Blue Mix LR 2000 Reactions

AzuraView GreenFast qPCR Blue Mix LR 5,000 Reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

AzuraView Probe Fast qPCR Mix Blue - Product Sheet

AzuraView™ Probe Fast qPCR Mix Blue

AZ-2601B by Azura Genomics is an inhibitor-tolerant 2× master mix formulated for probe-based real-time qPCR, including TaqMan® and molecular beacon assays, with a blue inert tracer dye for visual confirmation of pipetting and compatibility with standard and fast-cycling thermal cyclers.

AzuraView Probe Fast qPCR Mix Blue 100 reactions

AzuraView Probe Fast qPCR Mix Colorless 100 reactions

AzuraView Probe Fast qPCR Mix Blue 500 reactions

AzuraView Probe Fast qPCR Mix Colorless 500 reactions

AzuraView Probe Fast qPCR Mix Blue 2000 reactions

AzuraView Probe Fast qPCR Mix Colorless 2000 reactions

AzuraView Probe Fast qPCR Mix Blue 5000 reactions

AzuraView Probe Fast qPCR Mix Colorless 5000 reactions

Direct PCR Master Mixes

<h2>What Are Direct PCR Master Mixes?</h2>
&nbsp;
Direct PCR master mixes are specially formulated 2x PCR reagents designed to amplify nucleic acid targets directly from crude biological samples such as blood, buccal swabs, tissue punches, cultured cells, or other minimally processed material without a separate DNA or RNA extraction step. This is achieved through inhibitor-tolerant polymerase formulations, often combined with proprietary buffer additives that counteract the effects of hemoglobin, melanin, polysaccharides, and other common PCR inhibitors present in crude lysates.&nbsp;Choose a direct PCR master mix for high-throughput genotyping or rapid screening applications where eliminating the extraction step provides meaningful time and cost savings, provided the application tolerates the typically modest reduction in amplification efficiency compared to extracted-template PCR.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Direct PCR &amp; Fast Extraction Kits: Streamlined solutions such as the Flash-Extract&trade; PCR Kit and Accuris Fast Extraction PCR Kit that enable rapid PCR workflows from minimally processed samples, reducing preparation time and simplifying genotyping applications.</li>
<li>Genotyping Kits: Specialized products including the Azura&trade; Mouse Genotyping Kit and Accuris 1 Hour Mammalian Genotyping Kit, designed for reliable genotyping workflows using tissue, tail, ear, or other biological samples.</li>
</ul>
&nbsp;
<h2>How to Choose a Direct PCR Master Mix</h2>
&nbsp;
Sample Type Compatibility
Direct PCR formulations vary in the crude sample types they are validated for. Blood, tissue, cells, and swabs each present different inhibitor profiles, so confirm the master mix is validated for your specific sample type before adopting it for a new workflow.
Inhibitor Tolerance Mechanism
Inhibitor tolerance is typically achieved through a combination of enzyme engineering and buffer additives that bind or neutralize inhibitory compounds. The specific combination affects which inhibitor types, such as heme from blood or melanin from pigmented tissue, the mix is best suited to handle.
Lysis Step Requirements
Some direct PCR master mixes require a brief lysis step, such as a short heat or detergent treatment, before amplification, while others are formulated for direct addition of the crude sample to the PCR reaction. Confirm the workflow complexity matches your throughput needs.
Amplicon Size and Application
Direct PCR is well established for short-to-moderate amplicon genotyping and screening applications. Very long amplicons or applications requiring maximum yield from limited template may still benefit from a brief extraction step even when a direct-compatible mix is available.
Comparison to Extraction-Based PCR
Direct PCR master mixes generally show good performance for routine genotyping but may exhibit somewhat reduced sensitivity or yield compared to PCR from purified template. For applications where extraction-free convenience outweighs a modest performance trade-off, direct PCR offers substantial time savings, particularly at high sample throughput.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Direct PCR master mixes enable PCR directly from crude samples through heat-stable polymerases tolerant of inhibitor compounds and lysis buffer components, combined with optimized buffer systems designed to neutralize hemoglobin, melanin, polysaccharides, and other inhibitors commonly found in blood, tissue, and plant material. The time savings from eliminating extraction are most significant in high-throughput genotyping pipelines, where extraction across hundreds of samples can represent a substantial fraction of total workflow time. As with any inhibitor-tolerant formulation, performance can vary by specific sample matrix. Validating a direct PCR master mix against a small panel of representative samples before deploying it across a full study is considered good practice. Direct PCR master mixes are typically stored at -20&deg;C. Lot-specific Certificates of Analysis (CoAs) are available on request.&nbsp;
&nbsp;
Streamline your amplification workflow&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Direct PCR solutions.

Mouse Genotyping Kit - Product Sheet

Azura™ Mouse Genotyping Kit

AZ-1851 by Azura Genomics: mouse genotyping kit combining rapid lysis buffer (15-min extraction, no columns) with Azura 2× HS Red Mix for direct gel loading. Full workflow in ~1 hour. Available in 80-, 400-, and 2,000-reaction packs.

Azura Mouse Genotyping Kit 80 Reactions

Azura Mouse Genotyping Kit 400 Reactions

Azura Mouse Genotyping Kit 2000 Reactions

<h2>What is Chemicals as a reagent category?</h2>
&nbsp;
Chemicals covers the broad range of chemical and biochemical reagents used across molecular biology, cell biology, biochemistry, and general laboratory work: biochemically relevant compounds like vitamins, buffer salts, amino acids, and enzyme substrates; general laboratory chemicals including solvents, acids, bases, and inorganic salts; and research-grade specialty chemicals for specific applications. Unlike application-specific categories such as Epigenetics or Electrophoresis, which organize reagents by research workflow, Chemicals organizes by the nature of the material itself.
&nbsp;
<h2 dir="ltr">What you'll Discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents" title="Biochemical Reagents:">Biochemical Reagents:</a> High-quality metabolic inducers such as IPTG (G116), L-Arabinose (A2001-ZYM), and Cumate (CH065), along with essential buffers and enzyme stabilizers like TRIS (CH059), DTT (G025), and PMSF (G034).
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/inorganic-reagents-and-compounds" title="Inorganic Reagents &amp; Compounds:">Inorganic Reagents &amp; Compounds:</a> Ultra-pure essential chemical substances like Sodium Azide (CH052) designed for accurate laboratory buffer enhancement and sample conservation methodologies.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/detergents-and-surfactants" title="Detergents &amp; Surfactants:">Detergents &amp; Surfactants:</a> Enhanced cell lysis and denaturing agents featuring 20% SDS (CH100) and concentrated 10X Tris-Glycine-SDS Buffer (CH125) for protein electrophoresis setup.
</li>
</ul>
&nbsp;
<h2>How to navigate Chemicals</h2>
&nbsp;
Start with Biochemical Reagents for biologically relevant compounds
Biochemical Reagents covers the subset of the catalog most relevant to molecular and cell biology workflows: vitamins and cofactors, buffer salts and ready-to-use buffer solutions, amino acids, and other compounds with specific roles in biological systems.
Check purity grade requirements before ordering a research chemical
Research-grade chemicals vary in purity from technical grade to ACS-certified, HPLC-grade, and molecular biology grade, with the appropriate grade depending on how the chemical is used; cell culture and enzymatic applications generally require higher purity than non-biological analytical or general lab use.
Confirm whether your chemical needs are better served by a specialty category
Some research chemicals belong more naturally in application-specific sections of the catalog, such as chromatin immunoprecipitation buffers in Epigenetics or gel running buffers in Electrophoresis, while general-purpose chemicals and biochemical reagents not tied to a single workflow type belong in this Chemicals branch.
Consider bulk ordering for high-consumption research chemicals
Common research chemicals like buffer salts, solvents, and vitamins are used in large quantities by active research labs, and bulk pricing can represent meaningful savings on frequently replenished reagents.
Look for certificates of analysis when accuracy matters for your application
Quantitative applications, including analytical standards, enzyme kinetics studies, and defined cell culture supplementation, require chemicals with documented lot-specific purity and concentration, typically confirmed by certificate of analysis provided with each lot.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Research-grade purity designations for laboratory chemicals follow widely adopted convention systems including ACS grade (meets American Chemical Society purity specifications), HPLC grade (verified purity for chromatographic applications), and molecular biology grade (certified free from nuclease, protease, and other activities that would interfere with molecular biology applications). As of 2026, pre-made, ready-to-use solutions for common biochemical reagents like PBS, HEPES buffer, and Tris-HCl continue to see growing adoption over powder or concentrated formats in labs prioritizing convenience and reproducibility over reagent cost per use.
<a href="https://mbpinc.net/contact-us">Reach out to the friendly team at MBP</a> now to get a quote for your particular research needs.

Chemicals

B-Galactosidase

Unit Quantity

Storage Condition

Shipping condition

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

B-globin-CRE-IRES-dsRED-DLX2 Adenovirus

Shipping Condition

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
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<h2>Specifications context</h2>
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The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

BAC/PAC Isolation KIt Protocol

BAC/PAC Isolation Kit

BAC/PAC Isolation Kit (PD1311-00) by MBP rapidly purifies BAC, PAC, cosmid, and P1 DNA from small volumes of bacterial cultures using a modified alkaline lysis procedure adapted for spin columns. Available in 4 (PD1311-00), 50 (PD1311-01), and 250 (PD1311-02) prep sizes.

BAC/PAC Isolation Kit , 4 Preps

BAC/PAC Isolation Kit , 50 Preps

BAC/PAC Isolation Kit , 250 Preps

Sample Storage Container

<h2 dir="ltr">What are Urine Collection Products?</h2>
 Urine collection products for laboratory use are containers and preservative systems designed to collect, stabilize, and transport urine specimens for urinalysis (UA), urine culture (C&amp;S), nucleic acid-based testing (NAAT for STIs), cell-free DNA (cfDNA) liquid biopsy, and 24-hour timed collections. Formats include sterile cups (30&ndash;60 mL), preservative tubes (5&ndash;50 mL), vacuum-draw urine transfer tubes, 24-hour collection containers (2&ndash;3 L), and preservative-prefilled devices for ambient-temperature stabilization of DNA, RNA, and proteins. Choose urine collection products with an active preservative when specimens cannot be processed within 2 hours of collection, when samples must be shipped to a centralized lab, or when nucleic acid integrity over 24+ hours is required.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Sterile urine collection containers for routine diagnostic sampling</li>
<li dir="ltr" role="presentation">Urine transport tubes for secure handling and transfer workflows</li>
<li dir="ltr" role="presentation">Preservative urine tubes for culture and urinalysis stability</li>
<li dir="ltr" role="presentation">Midstream urine collection systems for contamination reduction</li>
<li dir="ltr" role="presentation">24-hour urine collection containers for metabolic testing workflows</li>
<li dir="ltr" role="presentation">Leak-resistant screw-cap urine tubes for safe transport</li>
<li dir="ltr" role="presentation">Graduated urine containers with volume markings for easy measurement</li>
<li dir="ltr" role="presentation">Pediatric urine collection solutions for low-volume sampling</li>
<li dir="ltr" role="presentation">Closed-system urine transfer devices for reduced exposure handling</li>
<li dir="ltr" role="presentation">General laboratory urine collection consumables for research use</li>
</ul>
<h2 dir="ltr"> How to Choose Urine Collection Products</h2>
 Application: Culture vs. NAAT vs. Nucleic Acid Research For culture (C&amp;S), use sterile cups or boric acid preservative tubes. For NAAT (STI panels for Chlamydia, Gonorrhea), use sterile cups or manufacturer-validated transfer tubes; confirm with your platform's IFU (e.g., Hologic Aptima Combo 2 uses a specific urine transport tube). For nucleic acid research (cfDNA, RNA, protein), use dedicated urine preservative tubes (Norgen, Streck) that stabilize analytes for up to 72 hours at room temperature. Volume: Random vs. 24-Hour Collection Random midstream urine (10&ndash;30 mL) is adequate for urinalysis, culture, and NAAT. Timed 24-hour collections (total volume 500&ndash;3000 mL) require large-volume containers with the appropriate preservative for the ordered panel. Mixing 24-hour containers with different preservatives is a common error&mdash;verify the correct preservative type with the laboratory before dispensing to the patient. Preservative Compatibility Boric acid tubes maintain bacterial counts for culture up to 24 hours at room temperature. Boric acid is NOT compatible with NAAT workflows&mdash;it inhibits PCR. For NAAT, use plain sterile containers or manufacturer-specific transfer tubes. For liquid biopsy cfDNA, Streck Cell-Free DNA Urine Preservative maintains cell integrity and prevents genomic DNA contamination for up to 72 hours. Sterility All urine containers intended for culture must be certified sterile. Containers for NAAT must be certified DNase-free and RNase-free to prevent nucleic acid contamination from the container. Confirm sterility and nuclease-free certification in the manufacturer's datasheet before use in molecular workflows. Automated Compatibility For high-throughput labs using automated urine analyzers (Sysmex UF-5000, Siemens CLINITEK), confirm that the collection tube format and cap design are compatible with tube-handling robotics. Copan UriSponge&reg; (FDA-cleared October 2024) is a boric-acid-free device validated for direct loading on WASP&reg; automation systems. 
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<h2 dir="ltr">Specifications Context</h2>
 Standard urine collection cups are manufactured from transparent polypropylene (PP) or polystyrene (PS) with wide-mouth, screw-cap lids. Sterile cups rated for culture meet ISO 7886 and are non-pyrogenic. Boric acid preservative tubes contain approximately 1.8% boric acid, stabilizing bacterial counts by creating a bacteriostatic environment. Norgen urine preservative tubes (5&ndash;50 mL, available in multiple sizes) stabilize DNA, RNA, microRNA, and protein from fresh urine for over 2 years at room temperature per manufacturer validation data. As of 2026, liquid biopsy studies using urine cfDNA routinely use Streck Cell-Free DNA Urine Preservative (3 mL per 30 mL urine) as the standard stabilization protocol in published clinical research protocols. 
Need help picking the right urine collection setup? <a href="https://mbpinc.net/contact-us">Contact MBP</a>, and we&rsquo;ll help you sort it out fast. No confusion, just the right fit for your workflow.

Urine Collection Products

5538-Tubes, Caps & Racks Catalog

5538-TDS-5538

BECKMAN: 13mm Plug Cap for IDS Beckman Specimen Automation Track (2nd Generation), Blue, 500/Bag

5538 by Globe Scientific — blue 13 mm polyethylene plug caps for the Beckman IDS Specimen Automation Track (2nd Generation). 500 caps per bag. Cost-effective OEM replacement for Beckman automation track consumables. Available from MBP Inc. worldwide.

Manufacture

United States

Texas

mbpinc