Brands

Pipette Controller

<h2 dir="ltr"> What Are Pipette Controllers?</h2>
 A pipette controller (also called a pipetting aid, motorised pipette filler, or pipette boy) is a battery-operated instrument that accepts and drives 1&ndash;100 mL glass or plastic serological pipettes, replacing the rubber bulb, Pi-pump, and manual mouth-pipetting techniques. A motorised rotating wheel or roller generates controlled airflow to aspirate and dispense liquid at variable speeds; a hydrophobic 0.2 &micro;m inline filter prevents liquid and aerosol from reaching the motor. Controllers accept all standard serological pipette diameters (1 mL to 100 mL) through a silicone or rubber cone adapter. They are the standard dispensing tool for media preparation, cell culture reagent transfer, serial dilutions in large volumes, and reagent bottle-to-flask transfers. The disposable consumables used in the instrument. A pipette controller is required for safe, ergonomic use of serological pipettes in any volume from 1 mL to 100 mL &mdash; the rubber bulb is not a recommended alternative.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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Electronic pipette controllers for precise liquid control
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Manual pipette controllers for routine laboratory use
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Lightweight ergonomic pipette controllers
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Adjustable speed pipette controllers for controlled dispensing
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Controllers compatible with glass and plastic serological pipettes
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Sterile-use pipette controllers for sensitive applications
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High-precision aspirating controllers for accurate volume handling
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</ul>
<h2 dir="ltr"> How to Choose a Pipette Controller</h2>
 Power Source: Battery vs Rechargeable vs Mains Battery-powered controllers (AA or rechargeable internal battery) provide cordless flexibility across the bench and BSC. Rechargeable lithium-ion controllers offer 8&ndash;10 hours of continuous use per charge and charge from a base stand. Mains-powered controllers provide continuous operation without charge interruption &mdash; preferred in high-throughput cell culture facilities. Speed Control Two-speed controllers offer slow (for precise fill) and fast (for rapid dispense) modes. Variable-speed controllers with a continuous-pressure or stepless trigger allow fine-tuned flow rate adjustment for viscous media, delicate cell suspensions, or filling against gravity into narrow-neck vessels. Filter: Inline 0.2 &micro;m Hydrophobic Filter A 0.2 &micro;m hydrophobic inline filter is the critical protection component &mdash; it prevents liquid from entering the motor during accidental over-aspiration. Filters should be replaced when liquid reaches the filter or when flow resistance increases noticeably; replacement filters are sold separately for most models. Universal Cone Compatibility Confirm the controller's cone adapter accepts your pipette brand's tip diameter. Most controllers use a silicone or rubber cone that accommodates 1&ndash;100 mL serological pipettes from Falcon, Globe Scientific, and Nest Scientific without adapters. Autoclavability Controllers intended for BSC and sterile cell culture use should have an autoclavable cone, nose piece, and filter assembly. They have autoclavable lower sections; the battery/electronic module is not autoclavable. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard pipette controllers accept serological pipette diameters from 1 mL (narrow shaft) through 50 mL and 100 mL (wide-body). Replacement 0.2 &micro;m hydrophobic filters are sold in packs; filter replacement interval is typically every 6&ndash;12 months or immediately after liquid ingress. It has a runtime of approximately 8 hours per charge on a single lithium-ion battery.&nbsp; 
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Control your workflow with ease&mdash;designed for smooth aspiration, consistent dispensing, and dependable performance at every step. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP Team now.</a>

AzuraPette REACH, Long-Neck Pipette Controller, w/ QuickStand™, 115V US

A6950 by Azura Genomics is the AzuraPette REACH™ long-neck motorized serological pipette controller with QuickStand™ retractable kickstand, pressure-sensitive speed control, 8-hour rechargeable battery, and 0.8 µm inline filter. 115V US. Designed for ergonomic pipetting in biosafety cabinets.

Manufacturer

Single Channel Pipettors

<h2 dir="ltr">What Are Single-Channel Pipettors?</h2>
 A single-channel pipettor is the single most widely used liquid handling instrument in molecular biology, cell biology, and clinical research. It transfers precise volumes between 0.1 &micro;L and 5,000 &micro;L, one well or tube at a time, using an air-displacement piston mechanism. The piston creates a partial vacuum that aspirates liquid into a disposable tip; advancing the piston fully dispenses the liquid. A spring-loaded tip ejector releases the used tip without hand contact for contamination control. Manual models depend on finger force and offer simple, battery-free operation; electronic models add programmable aspiration/dispensing speeds, multi-dispense modes, and up to 5 custom pipetting programs.&nbsp; A set of four manual single-channel pipettors (P10, P20, P200, P1000) covers 95% of molecular biology liquid handling; add electronic models for high-throughput repetitive work.
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<h2 dir="ltr">What you will find:</h2>
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Adjustable volume single-channel pipettors for flexible workflows
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Fixed volume pipettors for repeatable dispensing tasks
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Mechanical pipettors for standard lab use
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Ultra-low volume micropipettors for precise &micro;L handling
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Wide-range pipettors covering &micro;L to mL volumes
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Autoclavable pipettors for sterile environments
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Lightweight ergonomic pipettors for long sessions
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</ul>
<h2 dir="ltr"> How to Choose a Single-Channel Pipettor</h2>
 Volume Range: Build a Complete Bench Set A standard four-pipettor bench set covers P10 (1&ndash;10 &micro;L), P20 (2&ndash;20 &micro;L), P200 (20&ndash;200 &micro;L), and P1000 (100&ndash;1,000 &micro;L). For sub-microliter work (sequencing, single-cell), add a P2 (0.2&ndash;2 &micro;L). For large-volume dispensing (media prep, column loading), add a P5000 (1,000&ndash;5,000 &micro;L). Avoid selecting a volume range that places your target dispense below 20% of the maximum &mdash; accuracy degrades at the low end of any range. Manual vs Electronic Manual single-channel pipettors (Eppendorf Research Plus, Gilson PIPETMAN Classic, Rainin Pipet-Lite XLS+) are the standard for routine lab work: field-calibratable, autoclavable, and requiring no power source. Electronic single-channel pipettors (Eppendorf Research Pro, Rainin Pipet-Lite XLS+ Electronic) add: programmable aspiration/dispense speed (5 speeds), multi-dispense (repeat pipetting) mode, mix mode, dilution mode, and backlight LCD volume display. Electronic models reduce hand fatigue in workflows exceeding 200&ndash;300 tip changes per day. Tip Compatibility Three tip systems cover the market: standard conical (universal fit &mdash; fits Eppendorf Research Plus, Gilson PIPETMAN, Rainin non-LTS, Thermo, Sartorius/BioHit); Rainin LTS cylindrical (requires LTS-specific tips &mdash; lower force insertion); Eppendorf epT.I.P.S. proprietary (tightest fit for Eppendorf instruments). Select the tip system that matches your pipettor purchase and existing tip inventory before committing to a brand. Autoclavability and Chemical Resistance Manual pipettors from Eppendorf, Gilson, and Rainin have fully autoclavable lower sections (121&deg;C, 15 psi, 15 min). Electronic pipettors have autoclavable nose cones only &mdash; the electronic module cannot be autoclaved. Calibration and Service All single-channel pipettors are factory-calibrated per ISO 8655. Field recalibration tools are available for Eppendorf, Gilson, and Rainin to perform in-lab gravimetric verification without external service. 
<h2 dir="ltr"> Specifications Context </h2>
 ISO 8655 Part 2 accuracy spec for a 200 &micro;L air-displacement pipettor: &plusmn;0.8 &micro;L systematic error, &le;0.3 &micro;L random error (Eppendorf Research Plus specification at 200 &micro;L). Most manual pipettors weigh 70&ndash;120 g; electronic models weigh 150&ndash;220 g. Operating temperature range: typically 15&ndash;40&deg;C. Eppendorf Research Plus, Gilson PIPETMAN Classic, and Rainin Pipet-Lite XLS+ LTS remain the three dominant single-channel pipettor platforms in North American research labs.&nbsp; 
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Ready to find your new favorite lab tool? Don&rsquo;t just settle for any grip&mdash;find the one that fits your hand and your science. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Reach out to the MBP team</a> today for a customized quote.

AzuraPette Single Channel Pipette

A5200-100U by Azura Genomics is the AzuraPette™ single-channel adjustable pipette for the 10–100 µL range. Features Roto-Lock™ volume adjustment, reduced actuation forces, and a lightweight ergonomic body designed for prolonged pipetting sessions.

AzuraPette Single Channel Pipette, 10 to 100ul

AzuraPette Single Channel Pipette, 1-10 mL

AzuraPette Single Channel Pipette, 1.0 to 10ul

AzuraPette™ Single Channel Pipette, 100 to 1000ul

AzuraPette™ Single Channel Pipette, 20 to 200ul

AzuraPette™ Single Channel Pipette, 2.0 to 20ul

AzuraPette™ Single Channel Pipette, 0.2 to 2ul

AzuraPette™ Single Channel Pipette, 30 to 300ul

AzuraPette™ Single Channel Pipette, 5.0 to 50ul

AzuraPette™ Single Channel Pipette, 1-5 mL

SureStand™ Pipette Stand for 6 pipettes, up to four multi-channels, acrylic

AzuraPette™ Starter Kit, includes 1 Pipette Carousel and 4 Pipettes, 10ul, 20ul, 200ul, 1000ul

Multi-Channel Pipettors

<h2 dir="ltr">What Are Multi-Channel Pipettors?</h2>
 A multi-channel pipettor contains 8 or 12 piston-tip cone assemblies arranged in a single row at 9 mm centre-to-centre spacing &mdash; matching the well spacing of standard 96-well plates &mdash; allowing an operator to simultaneously dispense into an entire plate row (12-channel) or column (8-channel) with a single plunger depression. This reduces the time to fill a 96-well plate from ~96 individual dispenses to 12 (8-channel) or 8 (12-channel). For 384-well plates, 16-channel models or adjustable-spacing multichannel pipettors accommodate the 4.5 mm inter-well spacing. Multi-channel pipettors are the standard instrument for ELISA plate loading, serial dilution across 96-well plates, PCR master-mix dispensing, and cell-based assay setup. An 8-channel pipettor fills a 96-well plate in 12 strokes; a 12-channel pipettor fills it in 8 &mdash; choose based on whether your workflow is row-based or column-based.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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8-channel adjustable volume multi-channel pipettors
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12-channel high-throughput pipettors
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Mechanical multi-channel pipettors for routine use
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Autoclavable multi-channel pipettors
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Ergonomic lightweight pipettors for extended use
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Calibrated precision multi-channel pipettors
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Microplate-compatible pipettors for 96-well formats
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</ul>
<h2 dir="ltr"> How to Choose a Multi-Channel Pipettor</h2>
 8-Channel vs 12-Channel 8-channel pipettors (8 rows) are used when working column-by-column across a 96-well plate &mdash; the standard for PCR setup, ELISA, and serial dilutions where samples run down columns. 12-channel pipettors (12 columns) are used when filling row-by-row, common for reagent dispensing from reservoirs across all 12 columns simultaneously. Both configurations dispense the same 96 wells per plate but in different orientations. Volume Range Common multi-channel volume ranges: 0.5&ndash;10 &micro;L (for low-volume PCR or sequencing), 5&ndash;50 &micro;L, 10&ndash;100 &micro;L, and 20&ndash;200 &micro;L (the most widely used range for ELISA and cell-based assays), and 30&ndash;300 &micro;L. Select the range that places your most frequent dispense volume at 50&ndash;80% of the maximum for best accuracy. Manual vs Electronic Manual multi-channel pipettors (Eppendorf Research Plus Multichannel, Gilson PIPETMAN Multichannel) require no battery and are field-calibratable. Electronic multi-channel pipettors (Eppendorf Xplorer Multichannel, Rainin Pipet-Lite L8 LTS Electronic) add programmable speed, multi-dispense mode, and reduced plunger force &mdash; particularly valuable for high-throughput plate workflows. Tip Compatibility Multi-channel pipettors use the same tip collar geometry as single-channel models: standard conical for Eppendorf Research Plus and Gilson PIPETMAN (universal-fit 8-strip tips); LTS cylindrical for Rainin L8/L12 LTS (LTS 8-strip tips). Confirm tip format matches the multichannel instrument before ordering tip strips. Adjustable Tip Spacing Eppendorf Research Plus Move It and equivalent adjustable-spacing models allow spacing between channels to be set to 4, 6, 8, or 12 mm &mdash; enabling use with 24-well, 48-well, and 384-well plate formats without a dedicated instrument for each format. 
<h2 dir="ltr"> Specifications Context</h2>
 Multi-channel pipettors are subject to ISO 8655 Part 6 (multichannel piston-operated volumetric instruments). Standard accuracy specification for a 200 &micro;L 8-channel model is &plusmn;1.5 &micro;L systematic error; inter-channel CV should be &le;1.0% to ensure well-to-well reproducibility in plate-based assays. Autoclavable lower sections are standard on Eppendorf Research Plus and Gilson PIPETMAN multichannel models. Eppendorf Research Plus Multichannel and Rainin Pipet-Lite L8/L12 LTS remain the dominant manual multichannel platforms in North American research labs.&nbsp; 
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Tired of the one-well-at-a-time grind? Let&rsquo;s speed things up.<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">&nbsp;Reach out to the MBP team</a> today for a quote on the multi-channel system that fits your lab&rsquo;s pace.

AzuraPette™ 12-Channel Pipette

AzuraPette™ 12-Channel Pipette (A5212-10U) by Azura Genomics — 1.0–10 µL volume range, 12-channel, lightweight low-force design with Roto-Lock™ volume adjustment. Ideal for 96-well plate liquid-handling workflows.

AzuraPette™ 12-Channel Pipette, 1.0 to 10ul

AzuraPette™ 12-Channel Pipette, 20 to 200ul

AzuraPette™ 12-Channel Pipette, 2.0 to 20ul

AzuraPette™ 12-Channel Pipette, 30 to 300ul

AzuraPette™ Pipette Controller with QuickStand

AzuraPette™ Pipette Controller with QuickStand (A6080) by Azura Genomics — 6 oz, ambidextrous, 8-hour rechargeable battery (3.7 V/750 mAh), pressure-sensitive speed control, 0.45 µm aerosol filter, patented retractable QuickStand™ kickstand. For 10 mL, 25 mL, and 50 mL serological pipettes.

AzuraPette™ Pipette Controller, w/ QuickStand™

Replacement battery, lithium, 3.7V, 750mAh

Replacement filter, 0.45um, pack of 5

Replacement Charger for Electronic Pipette Controller

SYBR/Primer-Probe

<h2 dir="ltr"> What Is a SYBR/Primer-Probe Master Mix?</h2>
 A SYBR/Primer-Probe master mix is a 2x formulation positioned to support both dye-based (SYBR Green) and primer-probe (hydrolysis probe, TaqMan-style) detection chemistries, typically built on a common hot-start Taq, dNTP, MgCl2, and buffer backbone with formulation adjustments that accommodate either an intercalating dye or a labelled probe, depending on the assay. Choose this combined format when your lab runs both SYBR Green and probe-based assays and wants to standardise on fewer master mix products across the qPCR workflow.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="tosb50" data-start="110" data-end="247">One-step RT-qPCR kits that combine reverse transcription and real-time PCR in a single tube to streamline RNA quantification workflows.</li>
<li data-section-id="11s385w" data-start="248" data-end="363">Hot-start enzyme systems are designed to reduce non-specific amplification during reaction setup and thermal cycling.</li>
<li data-section-id="1sl7e5s" data-start="364" data-end="494">Probe-based detection chemistries for specific RNA target quantification in gene expression and pathogen detection applications.</li>
<li data-section-id="7bdacc" data-start="495" data-end="613">HiROX, LoROX, and NoROX formulation options to support compatibility with a wide range of real-time PCR instruments.</li>
<li data-section-id="19ytf2e" data-start="614" data-end="737">Optimized one-step workflows that reduce sample handling and help minimize contamination risk in high-throughput studies.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR/Primer-Probe Master Mix</h2>
 Assay Portfolio Mix Labs running a mix of SYBR Green gene expression assays and probe-based genotyping or pathogen-detection assays can benefit from standardising on a combined-compatible master mix line, reducing the number of distinct products to validate, store, and track across the facility. Reference Dye Configuration Confirm whether the specific product includes ROX or other passive reference dyes as standard, since probe-based assays in particular often rely on ROX for instrument normalisation, while some SYBR-only workflows may use ROX-free instruments. Validation Across Chemistries When adopting a combined-compatible master mix for the first time, validating performance separately for both a representative SYBR Green assay and a representative probe-based assay is recommended, since buffer optimisations that favour one chemistry slightly can sometimes trade off against the other. Primer and Probe Concentration Guidelines Typical primer-probe qPCR setups use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M; confirm the master mix manufacturer's recommended concentration ranges for both components when switching from a single-chemistry product. Throughput and Inventory Simplification For core facilities supporting multiple research groups with different assay types, a combined-compatible master mix can simplify inventory management and reduce the risk of using the wrong master mix for a given assay type. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard real-time PCR master mixes are 2x concentrated and contain dNTPs, MgCl2, and DNA polymerase as their common backbone; a SYBR/primer-probe combined format extends this backbone with formulation choices intended to perform acceptably whether the researcher adds SYBR Green dye for dye-based detection or a labelled probe for probe-based detection. In probe-based setups, primer pairs are commonly used at a final concentration around 0.5 &micro;M with probes at 0.25-0.5 &micro;M, while SYBR-based protocols may additionally include a small amount of random hexamer primer in RT-qPCR contexts. Real-time PCR master mixes of this type are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-yield&nbsp;SYBR/Primer-Probe Master Mixes for your lab.

AzuraQuant Probe 1-Step qPCR Mix - Product Sheet

MSDS

User Manual

AzuraQuant Probe 1-Step qPCR Mix HiRox

AZ-3901 AzuraQuant Probe 1-Step qPCR Mix HiRox by Azura Genomics is a 2× RT-qPCR master mix with high ROX reference dye, compatible with TaqMan® and Scorpions® probes, 1 pg–1 µg RNA input, 20 µl reactions, stored at −20°C.

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 500 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant Probe 1-Step qPCR Mix LoRox

AZ-4101 by Azura Genomics is the AzuraQuant Probe 1-Step qPCR Mix LoRox — a 2× hot-start RT-qPCR master mix with 20× AzuraSprint Reverse Transcriptase for one-tube RT-qPCR using TaqMan, Scorpions, or molecular beacon probes. Available from 100 to 10,000 reactions. LoRox formulation for low passive reference instruments.

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 500 reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 1,000 Reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 5,000 Reactions

AzuraQuant Probe 1-Step qPCR Mix NoRox

AZ-4301 by Azura Genomics is AzuraQuant™ Probe 1-Step qPCR Mix NoRox — a 2× RT-qPCR master mix containing Azura HS Taq and AzuraSprint thermostable RT. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Available in 100 to 10,000 reaction formats. No ROX passive reference dye.

AzuraQuant Probe 1-Step qPCR Mix No-Rox 100 Reactions

AzuraQuant Probe 1-Step qPCR Mix No-Rox 500 Reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 5,000 reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

Product Literature

AzuraQuant Probe Fast qPCR Mix HiRox

AZ-2701 by Azura Genomics — AzuraQuant Probe Fast qPCR Mix HiRox; 2× probe-based mix; high ROX passive reference; TaqMan®, Scorpions®, molecular beacon compatible; antibody hot-start; standard and fast cycling; 100–5000 rxn formats.

AzuraQuant Probe Fast qPCR Mix HiRox 100 reactions

AzuraQuant Probe Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 2,000 reactions

AzuraQuant Probe Fast qPCR Mix HiRox, 5,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox

AZ-2801 by Azura Genomics: AzuraQuant™ Probe Fast qPCR Mix LoRox — 2× master mix for TaqMan, Scorpions, and molecular beacon probe assays on standard and fast cycling instruments. Low ROX passive reference. Antibody hot-start. 100–10,000 rxn packs.

AzuraQuant Probe Fast qPCR Mix LoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 500 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 5,000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox

AZ-2901 by Azura Genomics is a 2x ready-to-use probe-based qPCR master mix formulated without ROX passive reference dye. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Designed for instruments that do not require ROX normalization, including Bio-Rad CFX, Roche LightCycler, and Qiagen Rotor-Gene. Available in 100 to 10,000 reaction formats.

AzuraQuant Probe Fast qPCR Mix NoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix NoRox 2000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 5000 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green 1-Step qPCR Mix - Product Sheet

AzuraQuant™ Green 1-Step qPCR Mix HiRox

AZ-3501 by Azura Genomics — AzuraQuant™ Green 1-Step qPCR Mix HiRox, 2× master mix + 20× AzuraSprint RT for one-step RT-qPCR directly from RNA. SYBR Green channel, HiRox for Applied Biosystems instruments. 20 µL reactions, −20°C storage, 18-month shelf life. Available 100–10,000 reaction packs.

AzuraQuant Green 1-Step qRT-PCR Kit Hi-Rox 100 Reactions

AzuraQuant Green 1-Step qPCR Mix HiRox 500 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant™ Green 1-Step qPCR Mix LoRox

AZ-3701 by Azura Genomics is a 2× one-step RT-qPCR master mix (low ROX) with a companion 20× thermostable AzuraSprint reverse transcriptase and non-inhibitory intercalating dye, enabling single-tube RNA quantification with post-amplification melt curve analysis on low-ROX compatible instruments.