Brands

<h2>What are plasmid maxiprep kits?</h2>
&nbsp;
A plasmid maxiprep kit scales SDS/alkaline lysis with silica-membrane spin columns, anion-exchange resin with gravity flow, or fully automated cartridge-based processing to 100-500 ml of overnight E. coli culture, recovering up to 400 ug per spin-column prep or 1-1.4 mg with high-yield anion-exchange chemistry, completable in 35-75 minutes depending on format. Related entities include EndoFree-type endotoxin removal for transfection, automated processors that eliminate manual centrifugation, and downstream applications including large-scale transfection, in vitro transcription/translation, and multiple parallel experiments. Choose Maxiprep when your application needs the largest routine plasmid yields (hundreds of micrograms to over 1 mg) from a single overnight culture.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Ultrafast 25-minute maxiprep kits drastically reduce processing times using specialized column technology&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Syringe-filter plasmid maxi units rapidly clear dense bacterial lysates using vacuum-free clarification systems&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid maxiprep systems for delivering transfection-grade DNA in sensitive mammalian cell lines</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-performance silica-column vectors optimized for maximum concentration and recovery of low and high-copy plasmids</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Gravity-flow anion-exchange assemblies without manual centrifugation steps, ensuring chromatography-level purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid maxiprep kit</h2>
&nbsp;
When maxiprep is the right scale
A maxiprep should be used when purifying plasmid from an overnight bacterial culture with a volume between 100-500 ml, when downstream needs exceed midiprep's 0.5-2 mg range - for example, large-scale transfection across many plates, in vitro transcription/translation requiring substantial template DNA, or banking DNA for multiple future experiments from one culture. If midiprep yields already meet your needs, the larger culture volume and processing time of maxiprep may not be justified.
Spin-column vs. anion-exchange vs. automated chemistry
Spin-column maxipreps (e.g., 60-minute procedures) recover up to roughly 400 ug per prep, while anion-exchange or high-yield chemistries can reach 1-1.4 mg from comparable culture volumes; automated cartridge-based processors can deliver endotoxin-free DNA in under 75 minutes without manual centrifugation, pouring in culture, and pressing start. Match chemistry to both your yield target and available equipment - automated processors reduce hands-on time but represent a larger equipment investment.
Endotoxin levels at maxi scale
Endotoxin concentrations tend to be higher in larger-scale preparations - standard maxipreps have been reported at 11-16 EU/ug, while EndoFree-type kits target below 0.1 EU/ug through additional washing, and "substantially endotoxin-free" is sometimes defined as under 100 EU/ug for less stringent applications. For transfection of highly sensitive cells, vaccination research, or gene therapy applications, confirm the maxiprep kit's specific endotoxin target rather than assuming "maxiprep" implies low endotoxin by default.
Lysate clearing and centrifuge requirements
Maxi-scale lysate clearing typically requires high-speed centrifugation of the full culture volume (100-500 ml) followed by a clearing column or filtration step; gravity-flow anion-exchange systems use folded filters to avoid this centrifugation, while automated processors handle clearing internally. Confirm your centrifuge has rotors compatible with 100-500 ml culture volumes before committing to a centrifugation-based maxiprep format.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for maxiprep selection are expected yield (400 ug for spin-column vs. 1-1.4 mg for high-yield/anion-exchange chemistry), endotoxin target relative to your transfection application's sensitivity, and lysate-clearing method relative to your centrifuge's capacity for 100-500 ml culture volumes. A maxiprep should specifically be used for culture volumes in the 100-500 ml range; smaller volumes are better served by <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;or&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>. Labs running large-scale transfection or in vitro transcription/translation programs typically standardize on either high-yield anion-exchange maxipreps or automated processors to balance yield, endotoxin level, and hands-on time. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;covers the full mini-to-maxi range, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a>&nbsp;link to chromosomal DNA workflows. Use MBP's&nbsp;<a href="https://mbpinc.net/quickorder">Quick Order tool</a>&nbsp;for case-quantity pricing on maxiprep kits or&nbsp;<a href="https://mbpinc.net/contact-us">contact the specialist team</a>&nbsp;for guidance on endotoxin levels and automation options for your transfection program.
<section class="mbp-contact-cta"></section>
&nbsp;
Scale-up workflow efficiency with MBP&rsquo;s dependable macro-extraction plasmid technologies. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> today to find the perfect match for your lab.

Maxiprep Kits

User Manual

ezFlow Endofree Plasmid Maxiprep

ezFlow Endofree Plasmid Maxiprep (PD1520-00) by MBP uses a proprietary Buffer RET system and ezBind™ matrix to remove endotoxin, proteins, and contaminants. Eluted with sterile water or TE buffer. Purified DNA is ion exchange resin residues free. Listed for transfection, restriction mapping, sequencing, gene therapy, and genetic vaccinations. Available in 2 (PD1520-00), 10 (PD1520-01), and 25 (PD1520-02) prep sizes.

Manufacturer

ezFlow Endofree Plasmid Maxiprep, 2 Preps

ezFlow Endofree Plasmid Maxiprep, 10 Preps

ezFlow Endofree Plasmid Maxiprep, 25 Preps

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

Product Literature

ezFlow Endofree Plasmid Midiprep

ezFlow Endofree Plasmid Midiprep (PD1420-00) by MBP uses a proprietary ezBind™ matrix for exclusive DNA binding. No chaotropic salts in buffer; purified DNA is guanidine/anion exchange resin residues free. Eluted with sterile water or Elution Buffer. Available in 2 (PD1420-00), 10 (PD1420-01), 25 (PD1420-02), and 100 (PD1420-03) prep sizes.

ezFlow Endofree Plasmid Midiprep, 2 Preps

ezFlow Endofree Plasmid Midiprep, 10 Preps

ezFlow Endofree Plasmid Midiprep, 25 Preps

ezFlow Endofree Plasmid Midiprep, 100 Preps

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

ezFlow Endofree Plasmid Miniprep Kit

ezFlow Endofree Plasmid Miniprep Kit (PD1220-00) by MBP uses a proprietary DNA binding system for high efficient reversible binding to a mini column. Proteins and impurities removed by wash buffer; eluted with sterile water or elution buffer. Available in 4 (PD1220-00), 50 (PD1220-01), and 250 (PD1220-02) prep sizes.

ezFlow Endofree Plasmid Miniprep Kit, 4 Preps

ezFlow Endofree Plasmid Miniprep Kit, 50 Preps

ezFlow Endofree Plasmid Miniprep Kit, 250 Preps

ezFlow Endofree Plasmid Miniprep Kit II

ezFlow Endofree Plasmid Miniprep Kit II (PD1222-00) by MBP uses a proprietary DNA binding system for high efficient reversible binding to a mini column. Proteins and impurities removed by wash buffer; eluted with sterile water or elution buffer. Available in 2 (PD1222-00), 50 (PD1222-01), and 250 (PD1222-02) prep sizes.

ezFlow Endofree Plasmid Miniprep Kit II, 2 Preps

ezFlow Endofree Plasmid Miniprep Kit II, 50 Preps

ezFlow Endofree Plasmid Miniprep Kit II, 250 Preps

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

MSDS

g-DNA Wash Buffer

D3004-2 by Zymo Research is a genomic DNA wash buffer for the final column wash step in Zymo Research gDNA purification kits, removing residual salts and PCR inhibitors before elution. Available in multiple volumes (50–400 ml) for labs with varying throughput.

Shipping Condition

Storage Condition

g-DNA Wash Buffer (100 ml)

g-DNA Wash Buffer (200 ml)

g-DNA Wash Buffer (250 ml)

g-DNA Wash Buffer (2 x 200 ml)

g-DNA Wash Buffer (50 ml)

Compound Microscopes

<h2>What Are Compound Microscopes?</h2>
&nbsp;
A compound microscope forms a magnified image through two sequential lens systems: the objective lens (positioned close to the specimen) provides the primary magnification; the eyepiece (ocular) lens further magnifies the image from the objective, producing a final image that the eye or camera receives. Magnification is the product of the objective magnification and the eyepiece magnification (e.g., 40x objective x 10x eyepiece = 400x total). The transmitted light illumination system (Koehler illumination) passes light from below through the thin specimen mounted on a glass slide, through the objective, and to the eyepiece. This optical configuration provides the highest resolving power of any routine light microscope type, with resolution determined by the numerical aperture (NA) of the objective and the wavelength of light used. Routine brightfield compound microscopes with 4x, 10x, 40x, and 100x (oil) objectives and 10x eyepieces cover 40x to 1000x total magnification. Phase contrast models add a phase ring in the condenser matched to each objective, enabling contrast of unstained transparent cells. Fluorescence models add an epi-fluorescence port and filter cube turret for imaging immunofluorescence-labeled sections and fluorescent markers. Choose a compound microscope for examination of specimens that have been prepared as thin slides, stained sections, or smears; it is the standard instrument for histology, clinical microbiology, hematology, and fixed-tissue fluorescence imaging.
&nbsp;
<h2 class="PDq2pG_selectionAnchorContainer" data-section-id="bxblbi" data-start="0" data-end="23">What You Will Find:</h2>
&nbsp;
<ul data-start="25" data-end="588" data-is-last-node="" data-is-only-node="">
<li data-section-id="wnrw34" data-start="25" data-end="136">
BioBlue&trade; and bScope&reg; Compound Microscopes for routine biological, educational, and laboratory microscopy.
</li>
<li data-section-id="1ybdsx8" data-start="137" data-end="238">
iScope&reg; Research Microscopes with Plan and E-Plan optics for high-quality imaging and analysis.
</li>
<li data-section-id="g8b9ar" data-start="239" data-end="350">
Phase Contrast Microscopes for enhanced observation of transparent, unstained specimens and living cells.
</li>
<li data-section-id="ijdsh0" data-start="351" data-end="446">
LED Fluorescence Microscopes for fluorescence-based cell and tissue imaging applications.
</li>
<li data-section-id="vrizlz" data-start="447" data-end="588" data-is-last-node="">
Specialized Systems, including polarization microscopes and anatomopathology microscopes for advanced research and diagnostic workflows.
</li>
</ul>
&nbsp;
<h2>How to Choose a Compound Microscope</h2>
&nbsp;
Brightfield vs. phase contrast vs. fluorescence
Brightfield compound microscopes are the entry-level configuration, adequate for stained tissue sections, Gram-stained bacterial smears, blood smears, and all applications where contrast comes from staining rather than optical manipulation. Phase contrast is required for observation of unstained, living, or lightly fixed cells, where inherent contrast is too low for brightfield, common in microbiology, parasitology, and cytology. Fluorescence (epi-fluorescence) is required for DAPI nuclear staining, immunofluorescence (IF) labeling, FISH assays, and fluorescent reporter protein imaging in fixed cells.
Objective quality: plan-achromat vs. plan-apochromat
Plan-achromat objectives correct for chromatic aberration at two wavelengths and are flat (plan) across the field, suitable for routine brightfield and basic fluorescence imaging. Plan-apochromat objectives correct for chromatic aberration at three or more wavelengths and provide superior color fidelity and fluorescence flatness across the full field, required for multi-channel fluorescence imaging, quantitative image analysis, and publication-quality fluorescence documentation. Plan-apochromat sets cost significantly more than plan-achromat sets; specify them when fluorescence image quality and accurate color reproduction matter.
Magnification set
A standard objective set for a research compound microscope: 4x (NA 0.10) for tissue overview; 10x (NA 0.30) for histological pattern assessment; 20x (NA 0.50) for cellular detail without immersion; 40x dry (NA 0.65 to 0.75) for detailed cell morphology; 63x or 100x oil immersion (NA 1.25 to 1.40) for highest-resolution organelle imaging and bacteria identification. For clinical microbiology, 100x oil is the standard for bacterial identification. For routine histology, 10x and 40x are the most used.
Illumination: LED vs. halogen
LED transmitted light illumination is now standard on most new compound microscopes, providing 50,000+ hour lamp life, instant-on operation, no color temperature shift over lifetime, and significantly lower power consumption than halogen. Halogen (6V 30W or 12V 100W) remains in use on older instruments and some entry-level models and requires bulb replacement every 50 to 200 hours. For new purchases, specify LED illumination.
Camera port and documentation
A trinocular head with a C-mount camera port is standard for documentation in research labs. The camera beam splitter on research-grade microscopes maintains eyepiece brightness while splitting a portion of the light to the camera; entry-level trinoculars may dim the eyepiece view when the camera port is in use. A CMOS or CCD camera is required; confirm the camera's pixel size against the objective magnification and required image resolution for your intended analysis.
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<h2>Specifications Context</h2>
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Oil immersion objectives (63x NA 1.40, 100x NA 1.40) require immersion oil between the objective front lens and the coverslip to achieve their rated NA. Confirm that the coverslip thickness is No. 1.5 (0.17 mm); most plan-apochromat objectives are corrected for this standard thickness. Non-standard coverslip thickness introduces spherical aberration that degrades resolution and fluorescence image quality. For routine use in clinical microbiology or hematology where high-throughput slide processing is needed, wide-field eyepieces (22 mm field number) reduce the number of microscope fields needed to survey the full slide area, improving throughput.&nbsp;
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<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>to discuss product availability or get selection guidance.

EIS-1152-PLPHI-Group-Microscopes Catalog

EIS-1152-PLPHI-Group-TDS-EIS-1152-PLPHI

EIS-1152-PLPHI-Group-TDS-EIS-1153-PLPHI

EIS-1152-PLPHI-Group-Choosing a Microscope Made Easy

iScope® for Life Science (Infinity Models) Specifications

iScope® Microscope for Phase Contrast

EIS-1152-PLPHI by Euromex is a binocular phase contrast microscope with four Plan Phase IOS objectives (10x–S100x oil), a Zernike rotating condenser (N.A. 1.25), and intensity-adjustable 3 W NeoLED™ Köhler illumination. The ergonomic 30° Siedentopf binocular head with 48–76 mm interpupillary adjustment and rackless mechanical stage makes it suitable for extended live-cell, microbiology, and teaching applications. Contact MBP for institutional pricing;

Manufacture

iScope binocular microscope with EWF 10x/22mm eyepieces, plan phase PLPHi 10/20/S40/S100x oil IOS objectives, Zernike phase contrast disc condenser with darkfield stop, rackless stage and Köhler 3W NeoLED™ illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, plan phase PLPHi 10/20/S40/S100x oil IOS objectives, Zernike phase contrast disc condenser with darkfield stop, rackless stage and Köhler 3W NeoLED™ illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, plan phase PLPHi 10/20/S40/S100x oil IOS objectives, Zernike phase contrast disc condenser with darkfield stop, rackless stage and Köhler 3W NeoLED™ illumination, with HD-Mini camera, color High definition 2MP high speed camera with 13 inch HD screen, 1280 x 1080p, stand-alone usage with standard 32GB SD card, HDMI output. With built-in mouse-driven capture software

EIS-1152-PLI-Group-Microscopes Catalog

EIS-1152-PLI-Group-TDS-EIS-1152-PLI

EIS-1152-PLI-Group-TDS-EIS-1153-PLI

EIS-1152-PLI-Group-Choosing a Microscope Made Easy

iScope® Microscope with Plan PLI

EIS-1152-PLI by Euromex is the iScope® binocular microscope with EWF 10×/22 mm eyepieces, quintuple reversed nosepiece, Plan IOS 2×/4×/10×/S40×/S100× oil objectives (20×–1000×), 3W NeoLED™ with iCare energy-saving sensor, PH/DF condenser slot, and large 187 × 230 mm rackless stage. CE marked, 10-year warranty.

iScope binocular microscope with EWF 10x/22mm eyepieces, plan PLi 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ Köhler illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, PLi plan 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ Köhler illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, PLi plan 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ Köhler illumination, with HD-Mini camera, color High definition 2MP high speed camera with 13 inch HD screen, 1280 x 1080p, stand-alone usage with standard 32GB SD card, HDMI output. With built-in mouse-driven capture software

EIS-1053-PLPOLRI-Group-Microscopes Catalog

EIS-1053-PLPOLRI-Group-TDS-EIS-1053-PLPOLRI

EIS-1053-PLPOLRI-Group-Choosing a Microscope Made Easy

iScope® Polarization Model Specifications

iScope® Polarization Microscope

EIS-1053-PLPOLRI by Euromex is the iScope® trinocular polarization microscope with Plan PLPOLRi IOS objectives (5x/10x/20x/S50x), 3W NeoLED™ Köhler illumination, 50W halogen epi-illumination, rotatable 60° polarizer, Bertrand lens, and a 160mm rackless round stage. Magnification 50x–500x.

iScope trinocular microscope with EWF 10x/20mm eyepieces, plan polarization PLPOLRi 5/10/20/S50x IOS objectives, 160mm round stage. Rotatable 60° polarizer and slider with analyzer. Bertrand lens. 3W NeoLED™ brightfield Köhler illumination and reflected attachment with 50W halogen epi-illumination

iScope trinocular microscope with EWF 10x/20mm eyepieces, plan polarization PLPOLi 5/10/20/S50x IOS objectives, 160mm round stage. Rotatable 60° polarizer and slider with analyzer. Bertrand lens. 3W NeoLED™ brightfield Köhler illumination and reflected attachment with 50W halogen epi-illumination, with HD-Mini camera, color High definition 2MP high speed camera with 13 inch HD screen, 1280 x 1080p, stand-alone usage with standard 32GB SD card, HDMI output. With built-in mouse-driven capture software

Microscopes - Catalog

Choosing a Microscope Made Easy

iScope®Microscope with E-Plan PLI

The EIS-1152-EPLI iScope® by Euromex is a binocular compound microscope with E-Plan EPLi IOS objectives (4×–100× oil), 40×–1,000× total magnification, EWF 10×/22 mm eyepieces, 3W NeoLED™ Köhler illumination, a 79×52 mm X-Y mechanical stage, and an iCare auto-shutoff sensor — 408×210×255 mm, 9.0 kg.

iScope binocular microscope with EWF 10x/22mm eyepieces, E-plan EPLi 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, E-plan EPLi 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ illumination

iScope trinocular microscope with EWF 10x/22mm eyepieces, E-plan EPLi 4/10/S40/S100x oil IOS objectives, rackless stage and 3W NeoLED™ illumination, with HD-Mini camera, color High definition 2MP high speed camera with 13 inch HD screen, 1280 x 1080p, stand-alone usage with standard 32GB SD card, HDMI output. With built-in mouse-driven capture software

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
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Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

jGCaMP7c-P2A-LOCa3 (coexpressing LOCa3 and a state-of-the-art green calcium indicator jGCaMP7c)

Unit Quantity

jRCaMP1b-P2A-LOCa3 (coexpressing LOCa3 and a state-of-the-art red calcium indicator jRCaMP1b)

mKate2-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)

Vector

United States

Texas

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