Brands

<h2>What are epigenetics dNTPs?</h2>
&nbsp;
Epigenetics dNTPs includes both standard deoxynucleotide triphosphate sets used across many molecular biology applications and modified nucleotide analogs relevant specifically to epigenetic research, with the most important being 5-methyl-dCTP, a base-modified analog in which the cytosine base carries a methyl group at the 5-carbon position. 5-methyl-dCTP can be incorporated into DNA via PCR or in vitro synthesis in place of standard dCTP, producing a defined, fully methylated cytosine-substituted DNA molecule useful as a positive control for methylation detection assays or as a substrate for studying methylation-related enzyme activity.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li><a href="https://mbpinc.net/reagents/epigenetics/dntps-deoxynucleotide-triphosphates/methylated">Methylated: </a>dNTP mixes used in methylation-sensitive DNA synthesis workflows.&nbsp;Methylated nucleotide analogs for DNA methylation studies and epigenetics research.&nbsp;Standard individual dNTPs (dATP, dCTP, dGTP, dTTP) for PCR analysis and routine DNA synthesis.</li>
</ul>
&nbsp;
<h2>How to choose epigenetics dNTPs</h2>
&nbsp;
Use 5-methyl-dCTP to build defined, fully methylated control DNA
Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction produces a fully methylated cytosine-substituted amplicon, providing a positive control whose methylation status is defined and known, unlike a positive control derived from genomic DNA with variable, unknown methylation patterns.
Confirm that your methylation detection assay is validated with a defined methylated control
Running a fully methylated positive control through a bisulfite conversion or enzymatic conversion workflow confirms the detection method is correctly interpreting methylated cytosines, helping catch conversion efficiency problems before they affect interpretation of real sample results.
Use methylated dNTP-synthesized DNA to study methylation-sensitive restriction digestion
Some restriction enzymes specifically cleave methylated sequences while others are inhibited by methylation, and a fully methylated DNA produced with 5-methyl-dCTP is a useful substrate for confirming whether a specific enzyme's activity is methylation-sensitive.
Be aware that 5-methyl-dCTP is also believed to be the starting substrate for TET enzyme oxidation
5-methylcytosine is believed to be the substrate for TET family enzymes that produce 5-hydroxymethylcytosine and further oxidation products, making 5-methyl-dCTP-synthesized DNA a potential substrate for in vitro TET enzyme activity studies.
Confirm nucleotide concentration and compatibility with your synthesis conditions
Standard dNTPs and modified analogs like 5-methyl-dCTP are typically supplied as concentrated solutions requiring dilution, and confirming appropriate concentration and buffer compatibility before use ensures consistent, reproducible incorporation into your target DNA.
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<h2>Specifications context</h2>
&nbsp;
5-methyl-dCTP is typically supplied as a 10 mM solution and offers the ability to enzymatically make a defined, fully methylated, cytosine-substituted DNA by PCR or in vitro synthesis, a property that makes it a valuable resource for a variety of biochemical and cellular applications in epigenetics research. As of 2026, methylated DNA probes made with 5-methyl-dCTP continue to serve as methylation reference fragments and as pull-down substrates for 5-hydroxymethylcytosine-binding proteins from cellular lysates, extending their use beyond simple positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find high-integrity dNTPs tailor-made for your research applications.

dNTPs (Deoxynucleotide Triphosphates)

dGTP (100 mM) (250 µl)

C2011-2 by Zymo Research — 2-pack of Air Permeable Sealing Covers for 96-well microcentrifuge spin-column and collection plates. Allows gas exchange while preventing cross-contamination and reducing evaporation during incubation steps. Compatible with Zymo-Spin I-96-XL Plates (C2010). Also available in 4-pack (C2011-4) and 8-pack (C2011-8).

Concentration

Storage

Manufacturer

Shipping Condition

Storage Condition

<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
</ul>
&nbsp;
<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

DataSheet

MSDS

dNTP Mix

Unit Quantity

Application

Product Features

Caution

Shipping condition

Protocol

dNTP Mix (10 mM)

dATP solution for use in nucleotide mixes, PCR, RT-PCR, library preps, etc.

Storage 

dNTP Mix (10 mM) (100 µl)

dNTP Mix (10 mM) (500 µl)

dNTP Mix, 10mM each

dNTP Mix (NT01-00) by MBP is a mixture of dATP, dCTP, dTTP and dGTP, each at 10 mM. Available in 50 µl (NT01-00), 500 µl (NT01-01), 2 mL (NT01-02), and 5 mL (NT01-03). Ships on ice.

dNTP Mix, 10mM each, 50 uL

dNTP Mix, 10mM each, 500 uL

dNTP Mix, 10mM each, 2 mL

dNTP Mix, 10mM each, 5 mL

<h2 dir="ltr">What is a dNTP Set?</h2>
 A dNTP Set is a complete set of individual, highly pure deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - each supplied as a separate 100 mM solution in water. Each nucleotide can be diluted and mixed with any of the other three to the desired final concentration or ratio, giving researchers full control over custom PCR, qPCR, or labelling mix preparation. Choose a dNTP Set when you need to prepare custom-concentration mixes, run multiple applications with different nucleotide requirements, or want individual stocks for non-equimolar formulations.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="color: #000000;" data-section-id="rmv9gj" data-start="173" data-end="310">Pre-mixed deoxynucleotide triphosphate (dNTP) sets for PCR and DNA synthesis applications, including PureStrand&trade; dNTP Set (Azura)</li>
<li style="color: #000000;" data-section-id="1u2l5t9" data-start="311" data-end="437">High-concentration nucleotide formulations such as Accuris 100mM dNTP Set for flexible reaction setup and optimization</li>
<li style="color: #000000;" data-section-id="kkyf1s" data-start="438" data-end="533">Standard dNTP set formats suitable for routine PCR workflows, including dNTP Set (G050)</li>
<li style="color: #000000;" data-section-id="1j5qt5c" data-start="534" data-end="653">Nucleotide triphosphate formulations for enzymatic and molecular biology applications, including NTP Set (G946)</li>
<li style="color: #000000;" data-section-id="1nhlqes" data-start="654" data-end="773">Ready-to-use balanced nucleotide sets supporting DNA polymerase activity in PCR and related amplification reactions</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a dNTP Set</h2>
 Custom Mix Preparation A dNTP Set's primary advantage is flexibility - preparing a working mix at any desired total concentration and ratio, including non-standard formulations for specific applications such as nucleotide analog substitution (e.g., dUTP in place of dTTP for anti-carryover-contamination strategies). Multiple Application Support Labs running PCR, qPCR, cDNA synthesis, and DNA labelling from the same stock benefit from a single dNTP Set, since each application may call for slightly different concentrations or ratios prepared from the same individual 100 mM tubes. Stock Concentration 100 mM is the standard individual stock concentration for dNTP Sets, providing substantial dilution headroom - a 100 mM stock can be diluted 1000-fold to reach a 0.1 mM working concentration, or combined at lower dilution ratios for higher-concentration premixes. Equimolar Mixing Discipline When preparing a standard equimolar mix from a dNTP Set, equal volumes of each 100 mM nucleotide are combined with water to the desired total concentration - for example, equal-volume mixing of all four 100 mM stocks yields a 25 mM-each premix at 100 mM total. Purity and Lot Testing PCR-grade dNTP Sets are typically tested at greater than 99% purity by HPLC, with concentration verified by optical density spectrometry and absence of human/E. coli DNA confirmed by qPCR - important documentation for labs requiring lot-specific quality records. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard equimolar working mix from a dNTP Set, combining equal volumes of each 100 mM individual stock yields a mix at 25 mM of each nucleotide (100 mM total); this can be further diluted to common working stocks such as 10 mM each (a 50x stock relative to the 0.2 mM PCR final concentration) or 2.5 mM each (a 10x stock). The performance of each lot is typically functionally tested in a PCR requiring all four nucleotides, with concentration verified by optical density spectrometry and purity confirmed by HPLC. dNTP Sets are stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing each individual tube thoroughly before use is recommended. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get reliable dNTP sets for your next successful experiment.

dNTP Set

dTTP (100 mM) (250 µl)

D1010 by MBP is a 100 mM dTTP (deoxythymidine triphosphate) solution supplied in a 250 µl volume, formulated for direct use in PCR, RT-PCR, nucleotide mix preparation, and library construction protocols. Store at -20°C.

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

dsDNA Shearase

dsDNA Shearase (E2019-200) by Zymo Research — dsDNA Shearase Plus endonuclease (1 U/µL, 200 U) bundled with DNA Clean &amp; Concentrator-5 (DCC-5). Fragments 250 ng human DNA to 100–500 bp at 42°C in 20 min; elute purified fragments in as little as 10 µL. Ready for NGS and MeDIP workflows.

Enzyme Inactivation

Unit Definition

dsDNA Shearase™ (200 U)

dsDNA Shearase™ Plus (50 U)

dsDNA Shearase Plus

dsDNA Shearase Plus (E2018-200) by Zymo Research — 1 U/µL, 200 U. Fragments 250 ng human DNA to 100–500 bp in 20 min at 42°C in 10 µL. Generates 5′-phosphate/3′-OH termini ready for NGS adapter ligation. Enzyme only; for the DCC-5 bundle see E2019-200.

dsDNA Shearase™ Plus (200 U)

PCR & qPCR Film

eXTReme™ 2 Seal

eXTReme™ 2 Seal (XTR2-100) by MBP is an aluminum foil plate sealing film with excellent chemical resistance and superior performance at ultra-low temperatures. Distinct from eXTReme Seal (XTR-100, clear adhesive). Available in standard (XTR2-100) and large (XTR2-LG-100) formats, 100 films/pack each. Ships ambient.

Temperature Range

eXTReme™ 2 Seal, PCR and robotics, -20°C to +120°C, 100 Sealing Films/Pack

eXTReme™ 2 Seal, Large, PCR and robotics, -20°C to +120°C, 100 Sealing Films/Pack

<h2 dir="ltr">What are light-sensitive sealing films?</h2>
 Light-sensitive sealing films are light-blocking microplate seals &mdash; typically aluminum foil or opaque pigmented polymer &mdash; used to protect photosensitive contents from light exposure during incubation, detection, and storage. They prevent the photobleaching of fluorophores and the photodegradation of light-labile reagents and drugs, while still stopping evaporation and well-to-well contamination. Foil versions also block gases and tolerate &minus;80 &deg;C storage, and several are pierceable for robotic access. Buy from this category whenever a sample's signal or stability depends on keeping light out of the wells.
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<h2 dir="ltr">What you will find: </h2>
<ul>
<li dir="ltr" aria-level="1">
Black opaque sealing films for full light protection
</li>
<li dir="ltr" aria-level="1">
Semi-opaque light-reducing films for controlled exposure workflows
</li>
<li dir="ltr" aria-level="1">
Standard formats for 96-well microplates (approx. 127 &times; 80 mm)
</li>
<li dir="ltr" aria-level="1">
384-well compatible sealing films for high-throughput assays
</li>
<li dir="ltr" aria-level="1">
Adhesive sealing films for secure microplate closure
</li>
<li dir="ltr" aria-level="1">
Optical-compatible films for fluorescence-based assays
</li>
<li dir="ltr" aria-level="1">
Pierceable and non-pierceable film options for workflow flexibility
</li>
<li dir="ltr" aria-level="1">
High-barrier films for evaporation and contamination control
</li>
</ul>
<h2 dir="ltr"> How to choose light-sensitive films</h2>
&nbsp;
Confirm full light-blocking Choose fully opaque foil or pigmented film, not tinted clear film, when contents are strongly photosensitive; foil blocks light completely, while pigmented polymer films balance light protection with easier handling.
Decide whether you still need optical access Light-sensitive films are not for reading through; if a protocol requires both light protection and optical detection, seal for storage with an opaque film and switch to an optical film at the read step.
Check temperature and storage rating Foil light-blocking films often double as cold storage seals rated to &minus;80 &deg;C, suiting light-sensitive compound libraries; confirm the stated range before archiving frozen samples.
<h2 dir="ltr"> Compare light-sensitive film variants.</h2>
&nbsp;
<ul>
<li data-section-id="17gvbe2" data-start="0" data-end="165">Opaque aluminum foil: Provides maximum light blocking and supports &minus;80 &deg;C storage; compatible with storage and polypropylene plates, as well as robotic probes.</li>
<li data-section-id="1jnkzrj" data-start="167" data-end="335">Pierceable foil: For photosensitive high-throughput screening (HTS) and automated sample access; compatible with polypropylene plates and liquid handling systems.</li>
<li data-section-id="1o0gauf" data-start="337" data-end="479" data-is-last-node="">Black/amber polymer film: For light-sensitive applications requiring easier benchtop handling; compatible with assay and culture plates.</li>
</ul>
<h2 dir="ltr"> Specifications context</h2>
 Light-sensitive films are characterized by opacity, temperature range, and whether they are pierceable. Aluminum foil offers complete light blocking plus a gas and moisture barrier, and often carries a &minus;80 &deg;C to +110 &deg;C rating for combined light protection and freezer storage. Pigmented polymer films are easier to handle for benchtop work. Because a conformable foil presses down into each well, it leaves a defined impression around every well that lets the user confirm a complete seal by eye, and the opaque, intact layer doubles as a tamper-evident and contamination barrier while plates move between the bench, incubator, and freezer. Because these films are not optically clear, they are removed before any fluorescence or absorbance read. As of 2026, they ship in packs of 100 or rolls in a low-to-mid price tier.&nbsp; 
&nbsp;
Instead of struggling with inconsistent results, choose a sealing solution designed specifically for light-sensitive work. <a href="https://mbpinc.net/contact-us">Connect with the MBP team</a> to find the right film format, compatibility, and bulk supply option for your application.

Light Sensitive Film

eXTReme™ FoilSeal™

eXTReme™ FoilSeal™ (XTR-FOIL-100) by MBP is an aluminum foil plate seal with excellent chemical resistance and superior performance in ultra-low temperatures. Listed for long-term storage, light sensitive assays, and robotics. &minus;80&deg;C to +120&deg;C, 100 films/pack. Standard (XTR-FOIL-100) and large (XTR-FOILLG-100) formats. Ships ambient.

eXTReme™ FoilSeal™, Long-term storage, light sensitive assays and robotics,-80°C to +120°C, 100 Sealing Films/Pack

eXTReme™ FoilSeal™, Large, Long-term storage, light sensitive assays and robotics,-80°C to +120°C, 100 Sealing Films/Pack

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
</ul>
&nbsp;
<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
&nbsp;
<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

eXTReme™ Seal

eXTReme™ Seal (XTR-100) by MBP is a clear, strong-adhesive PCR plate sealing film with chamfered corners to prevent overhang in robotic systems and plate stacks. Rated &minus;20&deg;C to +120&deg;C. Standard 96-well (XTR-100, 100 films) and large deep-well format (XTR-LG-100, replaces Thermofisher 4306311) available. Ships ambient.

eXTReme™ Seal, PCR and robotics, -20°C to +120°C, 100 Sealing Films/Pack

eXTReme™ Seal, LargeFor sealing PCR plates and deep well plates - Replaces 4306311 by Thermofisher 100 Seals/Case

eXTReme™ SealMate™ Roll

eXTReme™ SealMate™ Roll (RSMXTR2) by MBP is an extra-strong adhesive plate sealing film that minimizes evaporation yet removes cleanly without residue. Ideal for PCR, qPCR, ELISA, and storage applications. Available as roll only (RSMXTR2) or with SealMate applicator kit (SM-KIT-XTR).