Brands

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

MSDS

Zyppy Wash Buffer (Concentrate)

Zymo Research D4036-4 Zyppy™ Wash Buffer concentrate. Add ethanol 4:1 before use; 400 µl working buffer per miniprep column. Final wash step in Zyppy Plasmid Miniprep protocol. Compatible with Zyppy Miniprep Kits (D4036, D4019, D4020, D4037). Available in 6 mL, 12 mL, 24 mL, and 48 mL concentrate sizes. Room temperature storage. Made in USA.

Manufacturer

Zyppy™ Wash Buffer (Concentrate) (12 ml)

Zyppy™ Wash Buffer (Concentrate) (24 ml)

Zyppy™ Wash Buffer (Concentrate) (48 ml)

Zyppy™ Wash Buffer (Concentrate) (6 ml)

Protocol

Zyppy-96 Plasmid Kit

D4042 by Zymo Research is the Zyppy™-96 Plasmid Kit in 4 × 96 prep format (384 total), featuring a pellet-free 45-minute protocol that processes a full 96-well plate of E. coli cultures directly—no centrifuge pellet step required. Each well yields up to 5 µg of plasmid DNA at A260/280 ≥1.8 in ≥30 µl, suitable for sequencing, restriction digestion, and CRISPR construct screening.

Applicable For

Elution Volume

Equipment

Processing Time

Purity

Size Range

Yield

Shipping Condition

Storage Condition

Zyppy™-96 Plasmid Kit (4 x 96 Preps.)

Zyppy™-96 Plasmid Kit (8 x 96 Preps.)

<h2>What Are Magnetic Bead Plasmid DNA Extraction Kits?</h2>
&nbsp;
Magnetic bead plasmid DNA kits use superparamagnetic nanoparticles, typically silica-coated or anion-exchange-functionalized, suspended in optimized binding buffers to capture plasmid DNA from bacterial lysate. After alkaline lysis of E. coli culture (0.5-5 mL per reaction), the beads bind DNA; an external magnetic field then immobilizes the beads so supernatant, RNA, and proteins are washed away without centrifugation. The purified DNA is eluted in water or low-salt buffer and is ready for downstream use. Throughput scales from single-tube manual processing to 96-well plate automation on KingFisher Duo, MagnetaPure 32, or ASSIST PLUS liquid handlers. Choose magnetic bead kits when your lab runs plasmid minipreps at scale (24+ samples/run) or when centrifuge-free automation is required.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Magbio HighPrep Plasmid DNA Plus Kit for rapid, automated plasmid purification using high-binding paramagnetic particle technology.</li>
<li>Zyppy&trade;-96 Plasmid MagPrep Kit for scalable magnetic separation of high-purity vector constructs.</li>
<li>Zyppy-96 Plasmid MagBead Prep Kit for faster, column-free plasmid isolation.</li>
<li>Magnetic bead-based plasmid purification solutions compatible with high-throughput laboratory workflows.</li>
<li>Column-free plasmid DNA extraction systems that simplify processing and reduce hands-on time.</li>
<li>Products designed for efficient recovery of clean plasmid DNA for cloning, sequencing, and transfection applications.</li>
<li>Automation-friendly plasmid preparation kits suitable for 96-well and large-batch sample processing.</li>
</ul>
&nbsp;
<h2>How to Choose Magnetic Bead Plasmid Kits</h2>
&nbsp;
Automation Platform Compatibility
Bead-based kits are designed for specific magnetic rod or liquid-handler platforms. Confirm compatibility before ordering: NucleoMag Plasmid kits are validated for MagnetaPure and IsoPure Mini; MagJET kits are optimized for the KingFisher series; MagMAX Pro HT kits are designed for high-throughput centrifuge-free workflows. Using a kit outside its validated platform may require buffer volume adjustments and is not supported by the manufacturer.
Bead Type and DNA Binding Capacity
Silica-coated magnetic beads use chaotropic binding chemistry similar to spin columns; anion-exchange-functionalized beads offer higher selectivity for covalently closed circular (CCC/supercoiled) plasmid. Standard miniprep bead kits bind 5-20 ug from 1.5 mL culture; scaled protocols for 5 mL can yield up to 50 ug. Check binding capacity against your expected yield to avoid saturation.
High-Copy vs Low-Copy Plasmid
Most bead-based kits are validated for standard high-copy plasmids (pUC, pBR322 derivatives). Low-copy constructs such as fosmids or BACs require longer lysis times and may need protocol modifications; check the kit's validated plasmid types before purchasing for non-standard vectors.
Elution Volume and Concentration
Bead protocols typically elute in 50-100 uL. If downstream transfection requires high DNA concentration, choose kits that support low-volume elution (50 uL) or perform a secondary concentration step. Check that the eluate is compatible with your downstream assay buffer conditions.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Plasmid DNA purity from bead-based kits is evaluated by the A260/A280 ratio (target 1.8-2.0) and the A260/A230 ratio (&gt;1.8). Supercoiling percentage (measured by agarose gel densitometry) should exceed 80% for standard transfection applications. Automated 96-well bead workflows complete plasmid isolation in 30-60 minutes for a full plate, compared to 1-2 hours for centrifuge-based spin-column processing of the same number of samples. All kits are Research Use Only (RUO).
&nbsp;
<section class="mbp-contact-cta"></section>
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.

Mag Beads

MagBead

D4101 - MSDS

D4101-Protocol

D4101-AppNote-Hamilton

D4101-AppNote-Tecan

Zyppy-96 Plasmid MagBead Prep Kit

D4101 by Zymo Research: Zyppy™-96 Plasmid MagBead Prep Kit, 4×96 preps (384 total). Up to 5 µg/prep from 750 µl E. coli culture; ~60 min; centrifuge-free; ≥30 µl elution. Plasmids up to 25 kb. Compatible with robotic platforms and ZR-96 MagStand. Made in USA.

Zyppy™-96 Plasmid MagPrep Kit (4 x 96 Preps.)

Zyppy™-96 Plasmid MagPrep Kit (8 x 96 Preps.)

Zyppy™ Plasmid Miniprep Kit

D4019 by Zymo Research is a 100-prep plasmid miniprep kit using a pellet-free 8-minute direct lysis protocol. Inputs 600 µl E. coli overnight culture; yields up to 25 µg plasmid DNA (up to 25 kb) in ≥30 µl at A260/A280 ≥1.8 and ≤50 EU/µg endotoxin. Patented colored buffers provide visual lysis confirmation. Compatible with PCR, sequencing, cloning, and transfection.

Zyppy™ Plasmid Miniprep Kit (100 Preps.)

Zyppy™ Plasmid Miniprep Kit (400 Preps.)

Zyppy™ Plasmid Miniprep Kit (50 Preps.)

D4036 by Zymo Research is the Zyppy Plasmid Miniprep Kit (50 preps) — a pellet-free, 8-minute plasmid purification system isolating up to 25 µg of DNA from 600 µl of E. coli culture with A260/A280 ≥1.8 purity and ≤50 EU/µg endotoxin. Uses Zymo-Spin IIN columns; elution into ≥30 µl; handles plasmids up to 25 kb. Suitable for transfection, colony screening, sequencing, and cloning. Made in USA by Zymo Research.

Zyppy™ Plasmid Miniprep Kit (800 Preps.)

D4037 by Zymo Research is an 800-prep bulk plasmid miniprep kit that delivers up to 25 µg of plasmid DNA per preparation in just 8 minutes with A260/280 ≥1.8 and ≤50 EU/µg endotoxin—no pellet step required. The color-coded, pellet-free protocol is compatible with high- and low-copy E. coli strains and produces DNA suitable for sequencing, transfection, and restriction digestion.

DataSheet

Zyppy™-96 Plasmid Kit

A1004-ZYM by Zymo Research — 10 mg/ml Tetracycline Hydrochloride solution (≥ 98% purity) in 95% ethanol, ready to use at 10–20 µg/ml. Broad-spectrum bacteriostatic agent for E. coli antibiotic selection, plasmid maintenance, and BAC/PAC propagation. Store at –20°C. Available in 5 ml and 5 × 5 ml formats.

Zyppy™-96 Plasmid Kit (2 x 96 Preps.)

Deep Blue Lysis Buffer (30 ml)

Deep Blue Lysis Buffer (48 ml)

Neutralization/Clearing Buffer (100 ml)

Neutralization/Clearing Buffer (200 ml)

D4100-MSDS

D4100-1-MSDS

D4100-2-MSDS

D4100-3-MSDS

D4100-5-MSDS

D4100-Protocol

D4100-AppNote-Hamilton

D4100-AppNote-Tecan

Zyppy™-96 Plasmid MagPrep Kit

The D4100 by Zymo Research is a 2 × 96-prep Zyppy-96 Plasmid MagPrep Kit for magnetic bead-based high-throughput plasmid DNA purification, delivering up to 5 µg per well from plasmids up to 25 kb in 60 minutes with no centrifuge required. Endotoxin ≤50 EU/µg, A260/280 ≥ 1.8; compatible with liquid-handling robotics and downstream sequencing, restriction digestion, and transfection.

Zyppy™-96 Plasmid MagPrep Kit (2 x 96 Preps.)

MagClearing Beads (10 ml)

MagClearing Beads (20 ml)

MagClearing Beads (40 ml)

MagBinding Beads (6 ml)

MagBinding Beads (8 ml)

MagBinding Beads (16 ml)

MagBinding Beads (24 ml)

MagBead J (3 ml)

MagBead J (50 ml)

MagBead J (100 ml)

EZ-Methylation Magprep Beads (4 ml)

EZ-Methylation Magprep Beads (8 ml)

EZ-Methylation Magprep Beads (16 ml)

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
&nbsp;
Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Targeted XJa and XJb strains for faster recovery of proteins.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</li>
</ul>
&nbsp;
<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
&nbsp;
Restriction enzyme selection and buffer compatibility
Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.
Enzyme unit definitions and reaction scale
Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.
Plasmid purification grade
Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.
Dephosphorylation for preventing vector self-ligation
Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
&nbsp;
Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote for high-purity chemicals and reagents.

Chemicals/Reagents

a-Factor Mating Pheromone (500 µl)

Y1004-500 a-Factor Mating Pheromone by Zymo Research is a farnesylated dodecapeptide supplied at 1 mg/ml in methanol, 500 µl, purity ≥80% HPLC, MW 1630 Da. Induces G1 arrest and mating response in S. cerevisiae MATα cells; working concentration 0.5 µg/ml for BAR1Δ strains. Store at −20°C short-term or below −70°C long-term. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many more leading research institutes, serving labs across the United States, Canada, and internationally.

Concentration

Molecular Weight

Product Storage

Structure

Usage

<h2 dir="ltr">What Are Adenoviral Control &amp; Reporter Constructs?</h2>
 Adenoviral over-expression experiments typically include matched control constructs that use the same vector backbone, promoter configuration, and production workflow as the experimental construct, and are packaged and purified under equivalent conditions. This helps account for vector-related and adenovirus-associated innate immune responses that can influence cellular readouts independently of the transgene. Using appropriate null or reporter controls is essential for accurate interpretation of adenoviral transduction and expression data. Choose the tools that support accurate data interpretation.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
GFP Adenovirus Reporters: confirm transduction visually using high-titer viral particles expressing green fluorescent protein
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Beta-Galactosidase (LacZ) Adenoviruses: &beta;-gal markers derived from E. coli for standardized colorimetric and histochemical assays.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
pShuttle-LacZ Control Vectors: produce recombinant adenoviruses expressing LacZ.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Null Adenovirus Controls: empty viral particles for assessment of non-specific cellular responses during gene delivery.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
CMV Null Adenoviruses: used to identify promoter-specific metabolic effects. Contains CMV promoter without a transgene.
</li>
</ul>
<h2 dir="ltr"> How to Choose Adenoviral Control &amp; Reporter Constructs</h2>
 Empty Vector Matching An empty vector control &mdash; identical backbone, promoter, and packaging as the experimental adenoviral construct, with no transgene insert &mdash; establishes the baseline for adenoviral delivery effects independent of the over-expressed gene. Fluorescent Reporter Selection GFP and RFP fluorescent reporters allow tracking of transduction efficiency by microscopy or flow cytometry, matching the promoter configuration of experimental constructs. Luciferase Reporter Format Firefly luciferase and Renilla luciferase provide quantitative bioluminescent readouts; secreted NanoLuc reporters allow sampling of culture supernatant for repeated, non-destructive measurements over a time course. Immune Activation Considerations Because adenoviral delivery activates innate immune sensing in transduced cells independently of any transgene, an identical-backbone control is essential to distinguish transgene-specific effects from generalised adenoviral response. Production Workflow Matching Controls should be produced, packaged, and purified using the same protocol and timeline as experimental constructs to avoid batch-to-batch variability confounding comparisons. 
<h2 dir="ltr"> Specifications Context</h2>
 Adenoviral delivery can activate innate immune sensing in transduced cells, altering gene expression independently of the transgene; therefore, matched controls with the same Ad5 backbone and promoter configuration are required for valid interpretation of adenoviral over-expression experiments. MBP adenoviral control and reporter constructs include GFP adenovirus, &beta;-gal (LacZ) adenovirus, and null adenovirus systems, supporting qualitative and quantitative assessment of transduction efficiency. Using control and experimental constructs produced under consistent conditions reduces variability that can confound downstream comparisons across treatment groups. Recording vector identity and batch information supports reproducibility and troubleshooting. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today for the selection of matched adenoviral control and reporter constructs for your workflow.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

b-gal Adenovirus (E. coli)

Unit Quantity

Vector Map

Titer 

Compound Microscopes

<h2>What Are Compound Microscopes?</h2>
&nbsp;
A compound microscope forms a magnified image through two sequential lens systems: the objective lens (positioned close to the specimen) provides the primary magnification; the eyepiece (ocular) lens further magnifies the image from the objective, producing a final image that the eye or camera receives. Magnification is the product of the objective magnification and the eyepiece magnification (e.g., 40x objective x 10x eyepiece = 400x total). The transmitted light illumination system (Koehler illumination) passes light from below through the thin specimen mounted on a glass slide, through the objective, and to the eyepiece. This optical configuration provides the highest resolving power of any routine light microscope type, with resolution determined by the numerical aperture (NA) of the objective and the wavelength of light used. Routine brightfield compound microscopes with 4x, 10x, 40x, and 100x (oil) objectives and 10x eyepieces cover 40x to 1000x total magnification. Phase contrast models add a phase ring in the condenser matched to each objective, enabling contrast of unstained transparent cells. Fluorescence models add an epi-fluorescence port and filter cube turret for imaging immunofluorescence-labeled sections and fluorescent markers. Choose a compound microscope for examination of specimens that have been prepared as thin slides, stained sections, or smears; it is the standard instrument for histology, clinical microbiology, hematology, and fixed-tissue fluorescence imaging.
&nbsp;
<h2 class="PDq2pG_selectionAnchorContainer" data-section-id="bxblbi" data-start="0" data-end="23">What You Will Find:</h2>
&nbsp;
<ul data-start="25" data-end="588" data-is-last-node="" data-is-only-node="">
<li data-section-id="wnrw34" data-start="25" data-end="136">
BioBlue&trade; and bScope&reg; Compound Microscopes for routine biological, educational, and laboratory microscopy.
</li>
<li data-section-id="1ybdsx8" data-start="137" data-end="238">
iScope&reg; Research Microscopes with Plan and E-Plan optics for high-quality imaging and analysis.
</li>
<li data-section-id="g8b9ar" data-start="239" data-end="350">
Phase Contrast Microscopes for enhanced observation of transparent, unstained specimens and living cells.
</li>
<li data-section-id="ijdsh0" data-start="351" data-end="446">
LED Fluorescence Microscopes for fluorescence-based cell and tissue imaging applications.
</li>
<li data-section-id="vrizlz" data-start="447" data-end="588" data-is-last-node="">
Specialized Systems, including polarization microscopes and anatomopathology microscopes for advanced research and diagnostic workflows.
</li>
</ul>
&nbsp;
<h2>How to Choose a Compound Microscope</h2>
&nbsp;
Brightfield vs. phase contrast vs. fluorescence
Brightfield compound microscopes are the entry-level configuration, adequate for stained tissue sections, Gram-stained bacterial smears, blood smears, and all applications where contrast comes from staining rather than optical manipulation. Phase contrast is required for observation of unstained, living, or lightly fixed cells, where inherent contrast is too low for brightfield, common in microbiology, parasitology, and cytology. Fluorescence (epi-fluorescence) is required for DAPI nuclear staining, immunofluorescence (IF) labeling, FISH assays, and fluorescent reporter protein imaging in fixed cells.
Objective quality: plan-achromat vs. plan-apochromat
Plan-achromat objectives correct for chromatic aberration at two wavelengths and are flat (plan) across the field, suitable for routine brightfield and basic fluorescence imaging. Plan-apochromat objectives correct for chromatic aberration at three or more wavelengths and provide superior color fidelity and fluorescence flatness across the full field, required for multi-channel fluorescence imaging, quantitative image analysis, and publication-quality fluorescence documentation. Plan-apochromat sets cost significantly more than plan-achromat sets; specify them when fluorescence image quality and accurate color reproduction matter.
Magnification set
A standard objective set for a research compound microscope: 4x (NA 0.10) for tissue overview; 10x (NA 0.30) for histological pattern assessment; 20x (NA 0.50) for cellular detail without immersion; 40x dry (NA 0.65 to 0.75) for detailed cell morphology; 63x or 100x oil immersion (NA 1.25 to 1.40) for highest-resolution organelle imaging and bacteria identification. For clinical microbiology, 100x oil is the standard for bacterial identification. For routine histology, 10x and 40x are the most used.
Illumination: LED vs. halogen
LED transmitted light illumination is now standard on most new compound microscopes, providing 50,000+ hour lamp life, instant-on operation, no color temperature shift over lifetime, and significantly lower power consumption than halogen. Halogen (6V 30W or 12V 100W) remains in use on older instruments and some entry-level models and requires bulb replacement every 50 to 200 hours. For new purchases, specify LED illumination.
Camera port and documentation
A trinocular head with a C-mount camera port is standard for documentation in research labs. The camera beam splitter on research-grade microscopes maintains eyepiece brightness while splitting a portion of the light to the camera; entry-level trinoculars may dim the eyepiece view when the camera port is in use. A CMOS or CCD camera is required; confirm the camera's pixel size against the objective magnification and required image resolution for your intended analysis.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Oil immersion objectives (63x NA 1.40, 100x NA 1.40) require immersion oil between the objective front lens and the coverslip to achieve their rated NA. Confirm that the coverslip thickness is No. 1.5 (0.17 mm); most plan-apochromat objectives are corrected for this standard thickness. Non-standard coverslip thickness introduces spherical aberration that degrades resolution and fluorescence image quality. For routine use in clinical microbiology or hematology where high-throughput slide processing is needed, wide-field eyepieces (22 mm field number) reduce the number of microscope fields needed to survey the full slide area, improving throughput.&nbsp;
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>to discuss product availability or get selection guidance.

Microscopes - Catalog

Choosing a Microscope Made Easy

bScope® with EPI-fluorescence

bScope Microscope for LED fluorescence

The EBS-3153-PLFI bScope® by Euromex is a trinocular LED fluorescence compound microscope with 4 excitation channels (B/G/V/UV), Plan Fluarex PLFi IOS objectives (4×–100× oil), HWF 10×/22 mm eyepieces, 3W NeoLED™ Köhler transmitted illumination, and a 75×36 mm X-Y rackless stage — CE certified with a 10-year warranty.

Manufacture

bScope trinocular microscope for LED fluorescence, HWF 10x/22mm eyepieces and quintuple nosepiece with Plan Fluarex PLFi 4/10/S40/S100x oil infinity corrected objectives, 131 x 152/197mm stage with integrated mechanical 75 x 36mm rackless X-Y stage.3W NeoLED™ Köhler illumination and integrated power supply. Fluorescence attachment with 4 LED's for B, G, V and UV excitation, 4 filter sets. Supplied without rechargeable batteries

bScope® for Phase Contrast

bScope® Microscope for Phase Contrast

EBS-1153-PLPHI by Euromex is a trinocular bScope® compound microscope for phase contrast, fitted with Plan Phase IOS objectives (10×, 20×, 40×, 100× oil), HWF 10×/20mm eyepieces, and a Zernike A.N. 1.25 condenser for high-contrast visualisation of unstained specimens. The 3W NeoLED™ Köhler illumination system supports cordless operation, and the antimicrobial protective layer suits shared laboratory environments. CE certified with a 10-year warranty; available from MBP for labs across the USA and Canada.

bScope trinocular microscope, HWF 10x/20mm eyepieces and quintuple nosepiece with plan phase PLPHi 10/20/S40/S100x oil infinity corrected objectives, 131 x 152/197mm stage with integrated mechanical 75 x 36mm rackless X-Y stage. Zernike phase contrast condenser with iris diaphragm and filter holder, 3W NeoLED™ Köhler illumination and integrated power supply. Supplied without rechargeable batteries

bScope trinocular microscope, HWF 10x/20mm eyepieces and quintuple nosepiece with plan phase PLPHi 10/20/S40/S100x oil infinity corrected objectives, 131 x 152/197mm stage with integrated mechanical 75 x 36mm rackless X-Y stage. Zernike phase contrast condenser with iris diaphragm and filter holder.3W NeoLED™ Köhler illumination and integrated power supply. Supplied without rechargeable batteries, with CMEX-18 Pro, 18.0MP digital USB-3 camera with 1/2.3 inch CMOS sensor

United States

Texas

mbpinc