Brands

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
&nbsp;
<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
&nbsp;
<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
&nbsp;
Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

MSDS

Tetracycline

Unit Quantity

Concentration

Purity

Storage Condition

Shipping condition

DataSheet

Tetracycline Hydrochloride

A1004-ZYM by Zymo Research — 10 mg/ml Tetracycline Hydrochloride solution (≥ 98% purity) in 95% ethanol, ready to use at 10–20 µg/ml. Broad-spectrum bacteriostatic agent for E. coli antibiotic selection, plasmid maintenance, and BAC/PAC propagation. Store at –20°C. Available in 5 ml and 5 × 5 ml formats.

Product Storage

Working Concentration

Manufacturer

Shipping Condition

Tetracycline Hydrochloride (10 mg/ml in Ethanol) (5 x 5 ml)

Tetracycline Hydrochloride (10 mg/ml in Ethanol) (5 ml)

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
</ul>
&nbsp;
<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
&nbsp;
<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

PCR & qPCR Film

ThermalSeal RTS Optically Clear, Silicone Adhesive

ThermalSeal RTS™ (TSS-RTQ-100) by MBP is an optically clear, silicone-adhesive plate seal for qPCR, long-term storage, protein crystallography, and HPLC. Direct alternative to Thermofisher 4311971 MicroAmp Optical Adhesive Film. Available in 100-seal (TSS-RTQ-100), 25-film (TSS-RTQ-25), and sterile 50-film (TSS-RTQS-50, &minus;80&deg;C to +105&deg;C) formats. Ships ambient.

Application

Temperature Range

Optical Adhesive Films For PCR, Storage & Crystallization - Replaces 4311971 by Thermofisher 100 Seals/Case

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, 25 Sealing Films/Pack

ThermalSeal RTS™, Optically Clear, Silicone Adhesive, Sterile, qPCR, long-term storage, protein crystallography & HPLC, -80°C to +105°C, 50 Sealing Films/Pack

ThermalSeal® 2™ and ThermalSeal® A™

ThermalSeal® 2™ and ThermalSeal® A™ (TS2-100 / TSA-100) by MBP feature a soft, extra thick, conformable adhesive for a very strong seal around raised wells. Listed for PCR, &minus;40&deg;C to +120&deg;C, 100 films/pack each. Ships ambient.

ThermalSeal2™, PCR, -40°C to +120°Cl, 100 Sealing Films/Pack, 100 Sealing Films/Pack

ThermalSeal® Advanced, PCR, -40°C to +120°C, 100 Sealing Films/Pack

Product Literature

ThermalSeal® RT2 for Optically Clear for Flat-Top Plates

ThermalSeal® RT2 (TS-RT2-100) by MBP is an optically clear plate seal for qPCR and real-time fluorescence detection through the seal during thermocycling. Rated &minus;40&deg;C to +120&deg;C. Direct alternative to Bio-Rad MSB1001 Microseal. Available for flat-top (TS-RT2-100) and raised rim (TS-RT2RR-100) plates, 100 films/pack each. Ships ambient.

ThermalSeal RT2™, Optically Clear for Flat-Top Plates, qPCR, -40°C to +120°C, 100 Sealing Films/Pack

ThermalSeal RT2RR™, Optically Clear for Raised Rim Plates, qPCR, -40°C to +120°C, 100 Sealing Films/Pack

ThermalSeal®, PCR and general storage

ThermalSeal® PCR and General Storage (100-THER-PLT) by MBP is a moderate-strength adhesive plate seal rated &minus;40&deg;C to +120&deg;C. Suitable for PCR thermocycling and freeze storage. Easy removal, no residue. Available non-sterile (100-THER-PLT) or sterile (STR-THER-PLT), 100 films/pack each. Ships ambient.

ThermalSeal®, PCR and general storage, -40°C to +120°C100 Sealing Films/Pack

ThermalSeal®, Sterile, PCR and general storage, -40°C to +120°C, 100 Sealing Films/Pack

<h2>What Are DNA-Modifying Enzymes?</h2>
&nbsp;
DNA-modifying enzymes catalyze defined chemical changes to DNA molecules: T4 DNA Ligase seals nicks and joins fragments; T4 Polynucleotide Kinase (PNK) transfers a phosphate from ATP to 5'-OH termini; alkaline phosphatase removes 5'-phosphate groups to block self-ligation; Klenow fragment fills in 5' overhangs and removes 3' overhangs to generate blunt ends; DNA methyltransferases add methyl groups to adenine or cytosine residues for epigenetic studies or protection from restriction digestion. These enzymes are distinct from PCR polymerases (which amplify DNA) and restriction enzymes (which cleave at specific sequences). Use DNA-modifying enzymes any time you need to alter DNA end chemistry, join fragments, or protect sequences from downstream enzymatic cleavage.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Thermolabile UDG: An essential "cleanup" enzyme that removes carryover contamination in PCR by breaking down uracil-containing DNA, thus guaranteeing your results stay clear of artifact-induced sequence bias.
</li>
<li dir="ltr" aria-level="1">
T4 DNA Ligase: The crucial molecular adhesive, designed for the swift and efficient connection of blunt or cohesive-end DNA pieces in cloning and NGS adapter ligation.
</li>
<li dir="ltr" aria-level="1">
T4 Gene 32 Protein: A unique single-stranded DNA binding protein that maintains open templates, greatly enhancing the efficiency of enzymes in challenging amplification and recombination tests.
</li>
</ul>
&nbsp;
<h2>How to Choose DNA-Modifying Enzymes</h2>
&nbsp;
Ligation format
T4 DNA Ligase is the standard choice for cohesive-end and blunt-end ligation in cloning. For high-throughput library prep, choose ligase formulations optimized for 25 degrees C or room-temperature reaction to reduce protocol time. E. coli DNA Ligase uses NAD+ (not ATP) and is preferred for nick sealing in nick-translation reactions.
End repair requirements
Klenow fragment (3' to 5' exo-) fills 5' overhangs and removes 3' extensions to generate blunt ends required for blunt-end cloning or adaptor ligation; its 3'-&gt;5' exonuclease-minus version is used in NGS A-tailing steps to add a single 3'-dA overhang. T4 DNA Polymerase has stronger 3'-&gt;5' exonuclease activity than Klenow and is preferred for end polishing of PCR products with heterogeneous ends.
Phosphorylation/dephosphorylation
Use T4 PNK to add 5'-phosphates to PCR products or synthetic oligonucleotides before blunt-end ligation. Use alkaline phosphatase (CIP or SAP) on linearized vectors to prevent self-ligation; SAP is preferred when heat-inactivation at 65 degrees C is needed before downstream steps. Combine dephosphorylation and ligation in the same tube only after inactivating the phosphatase.
Methylation and protection
CpG methyltransferases and dam/dcm methylases protect recognition sequences from restriction cleavage or enable methylation-sensitive mapping. Dam methylase (methylates GATC sequences) and Dcm methylase (methylates CCWGG) are used to prepare templates resistant to DpnI digestion in cloning workflows where DpnI is used to remove template plasmid.
Purity certification
For RNA co-purification or RNA-sensitive workflows, confirm all DNA-modifying enzymes carry RNase-free certification. ABM lot-release QC includes RNase and DNase contamination tests; certificates of analysis are available on request through MBP.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
T4 DNA Ligase activity is defined as the amount of enzyme catalyzing 50% ligation of HindIII-cut lambda DNA fragments in 30 minutes at 16 degrees C in a 20 microliter reaction; 1 Weiss unit equals approximately 60 cohesive-end units. Optimal blunt-end ligation uses PEG 4000 at 15% (w/v) as a molecular crowding agent and runs at 25 degrees C to increase effective enzyme concentration. Streamlined NGS library prep workflows combine end repair, A-tailing, and adaptor ligation into a single-tube step, reducing total hands-on time to under 1 hour.&nbsp;
&nbsp;
Seize control of your DNA processes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a quote on our premium DNA-altering enzymes.

DNA-modifying enzymes

Thermolabile UDG

Thermal Labels

<h2 dir="ltr">What Are Laboratory Thermal Labels?</h2>
 Laboratory thermal labels are adhesive labels designed for printing by direct-thermal or thermal-transfer desktop label printers &mdash; the two print technologies used for barcode and specimen ID generation in research, clinical, and diagnostic labs. Direct-thermal labels activate a heat-sensitive coating to produce an image without ink or ribbon; thermal-transfer labels use a resin or wax ribbon to fuse ink into the label surface. Thermal labels cover a wide range of container types: cryo vials, microcentrifuge tubes, PCR strips, 15/50 mL conicals, microscope slides, and specimen collection bags. Choose thermal-transfer labels for any sample requiring chemical resistance, temperatures above +50 &deg;C, or long-term barcode legibility; choose direct-thermal for low-cost, short-to-medium-term applications.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Direct thermal labels for quick, ink-free sample identification.
</li>
<li dir="ltr" aria-level="1">
Thermal transfer rolls for high-durability, permanent barcode printing.
</li>
<li dir="ltr" aria-level="1">
Solvent-resistant labels that withstand alcohol and cleaning agents.
</li>
<li dir="ltr" aria-level="1">
Freezer-grade thermal stickers for stable low-temperature archiving.
</li>
<li dir="ltr" aria-level="1">
Perforated label rolls for easy tearing and rapid application.
</li>
<li dir="ltr" aria-level="1">
Various sizes tailored for microtube caps and side-wall labeling.
</li>
</ul>
<h2 dir="ltr"> How to Choose Laboratory Thermal Labels</h2>
 Print Technology Compatibility Confirm whether the printer is direct-thermal (no ribbon required, e.g., Zebra GK420d, DYMO LabelWriter) or thermal-transfer (ribbon required, e.g., Zebra ZT230, Datamax M-Class). The two label types are not interchangeable &mdash; a thermal-transfer label will not print correctly in a direct-thermal printer and vice versa. Temperature and Chemical Exposure Direct-thermal label coatings degrade above approximately 50&ndash;60 &deg;C and with prolonged UV exposure, limiting their use to room-temperature and short-term cold storage applications. Thermal-transfer labels with a resin ribbon produce output resistant to temperatures up to +100 &deg;C, ethanol, xylene, and sanitizing wipes &mdash; required for autoclave tracking, histology, and GLP-regulated sample management. Container and Size Match Label dimensions must match the printable area of the label and the geometry of the container. Common lab thermal label widths are 0.5&ndash;2 inches; lengths are 0.25&ndash;3 inches. Verify dimensions against the container's flat-printable surface, accounting for any wrap-around on curved vials. Roll Core and Printer Compatibility Standard roll cores are 1-inch inner diameter for compact desktop printers; 3-inch cores fit high-volume industrial label printers. Confirm the printer's maximum roll diameter and label width capability before selecting roll format. Material: Paper vs. Polyester Paper-based thermal labels cost less and are suitable for ambient-temperature, short-duration applications (reagent dating, sample intake). Polyester thermal labels are moisture-resistant, more tear-resistant, and rated for demanding environments including freezers and chemical splash &mdash; specified for long-term specimen tracking and any application where label durability is a safety or compliance requirement. 
<h2 dir="ltr"> Specifications Context</h2>
 Direct-thermal label paper weight is typically 58&ndash;80 g/m&sup2;; polyester variants are 50&ndash;100 &micro;m thick. Standard roll dimensions are 2.25 inches &times; 1.25 inches (common for specimen tubes) and 4 inches &times; 6 inches (common for shipping and larger specimen bags). Print resolution for lab barcode labels should be 300 dpi for 1D/2D barcodes on labels smaller than 1 inch &times; 0.5 inch &mdash; 203 dpi produces legible output on larger-format labels.&nbsp;
&nbsp;
Stop struggling with faded barcodes. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Ask the MBP Team</a> for a quote right now, we help you make your data permanent.

Thermometer Strips

112214 by Globe Scientific are self-adhesive liquid crystal thermometer strips that display specimen temperature in both Fahrenheit (90°F–100°F) and Celsius (32°C–38°C). Designed for immediate attachment to urine collection cups and drug-testing containers to verify specimen validity at point of collection. Available in bags of 500.

Thermometer Strips, Graduated in both Fahrenheit (90-100°) and Celsius (32-38°), BAG/500

Thermometer Strips, Graduated in both Fahrenheit (90-100°) and Celsius (32-38°), CS/4000

ThinSeal™, 100 Sealing Films/Pack

ThinSeal™ (100-THIN-PLT) by MBP is a thin, optically clear adhesive plate sealing film for microplates. Minimizes optical interference for plate reader and fluorescence scanner workflows. 100 films/pack. Ships ambient; store at room temperature.

ThinSeal™, Sterile. 100 Sealing Films/Pack

ThinSeal™ Sterile (STR-THIN-PLT) by MBP is the sterile format of ThinSeal plate sealing film. 100 sealing films per pack. Single SKU. Ships ambient. Contact MBP for additional specifications.

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
50mL Vacuum Filtration Units for small-scale sterile sampling
</li>
<li dir="ltr" aria-level="1">
150mL Vacuum Filtration Systems for rapid reagent preparation
</li>
<li dir="ltr" aria-level="1">
250mL Filtration Units for routine buffer sterilization
</li>
<li dir="ltr" aria-level="1">
500mL Vacuum Filtration Cups for mid-scale media processing
</li>
<li dir="ltr" aria-level="1">
1000mL Large Volume Filtration for industrial-scale batching
</li>
<li dir="ltr" aria-level="1">
Sterile Bottle-Top Filters for secure aseptic transfer
</li>
</ul>
<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
&nbsp;
Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Flyer

Timer, 4 Channel, Count Up-Count Down

Globe Scientific 4-Channel Lab Timer (SKU 1004) — 4 independent count-up/count-down channels; 6-digit LCD; 99 hr, 59 min, 59 sec range; 70 dB alarm per channel; memory function; time-of-day clock. Powered by 1.5V LR44 battery (included). Dimensions: 2.75 × 2.625 × 1 in.

Manufacture

Automated Purification Systems Accessories

<h2>What Are Automated Purification System Accessories?</h2>
&nbsp;
Automated purification system accessories include consumables and support components used with automated nucleic acid purification platforms. Common accessories include tip combs, magnetic tip combs, deep-well plates, elution plates, reagent vessels, sample racks, and system control devices. These components support sample preparation, reagent handling, magnetic bead-based purification workflows, and routine instrument operation. Choose Automated Purification System Accessories to support efficient magnetic bead-based purification, minimize workflow interruptions, and maintain reproducible results.
&nbsp;
<h2>What You Will Find:</h2>
&nbsp;
<ul data-start="25" data-end="537" data-is-last-node="" data-is-only-node="">
<li data-section-id="q24m79" data-start="25" data-end="130">Tip combs and magnetic tip combs for automated magnetic bead-based nucleic acid purification workflows.</li>
<li data-section-id="5lldu5" data-start="131" data-end="229">Deep-well and elution plates designed for sample processing, washing, and nucleic acid recovery.</li>
<li data-section-id="by507b" data-start="230" data-end="325">Reagent vessels for automated handling of buffers, wash solutions, and purification reagents.</li>
<li data-section-id="u7msh5" data-start="326" data-end="420">Sample racks and support components for organized workflow setup and consumable positioning.</li>
<li data-section-id="dj84rx" data-start="421" data-end="537" data-is-last-node="">Platform-specific accessories and control devices that support routine operation of IsoPure&trade; purification systems.</li>
</ul>
&nbsp;
<h2>How to Choose Accessories for Automated Purification Systems</h2>
&nbsp;
Tip combs and magnetic tip combs
Tip combs and magnetic tip combs are used during automated magnetic bead handling and nucleic acid purification workflows. Select accessories designed for the specific purification platform to ensure proper fit, alignment, and operation.
Deep-well and elution plates
Deep-well plates are used for sample processing and reagent handling, while elution plates are used to collect purified nucleic acids following extraction. Confirm plate format and compatibility with the instrument and workflow requirements before ordering.
Reagent vessels and sample racks
Reagent vessels hold buffers, wash solutions, and other workflow reagents required during automated purification procedures. Sample racks provide organized positioning of vessels and consumables to support efficient workflow setup and operation.
System accessories
Additional accessories, including preconfigured control tablets and demonstration kits, support instrument operation, training, workflow development, and system setup activities.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Automated purification workflows depend on the correct combination of consumables and instrument accessories. Tip combs, plates, reagent vessels, and racks should be selected according to the instrument model and workflow format to ensure reliable sample handling and purification performance. Routine replacement of consumable components helps maintain workflow consistency and minimizes the risk of cross-contamination.
&nbsp;
To discuss product availability or receive guidance on accessory selection, <a href="https://mbpinc.net/contact-us">contact the MBP team.</a>

Automated Purification System Accessories

Tip Comb for IsoPure™ 96, cs 50

AP1096-TC by Accuris Instruments is a case of 50 tip combs designed for the IsoPure™ 96 Automated Purification System (AP1096). Each tip comb fits the AP1096's 96-position carousel and transfers magnetic beads through wash and elution steps in automated nucleic acid purification runs. Single-use per run; 50 combs per case for sustained high-throughput operation.