Name: ThermalSeal® RT2 for Optically Clear for Flat-Top Plates
SKU: TS-RT2-100
Availability: InStock

<h2 dir="ltr">What are PCR and qPCR sealing films?</h2>
 PCR and qPCR sealing films are optically clear microplate seals applied to 96- and 384-well PCR plates to create an airtight barrier through every thermal cycle, preventing evaporation, condensation, and cross-well contamination while still letting fluorescence pass through to the qPCR detector. Most are pressure-sensitive adhesive (PSA) or heat-activated films built on polypropylene or polyester (PET) with low-autofluorescence chemistry. Standard formats fit SBS sheets of approximately 137 &times; 80 mm. Buy from this category whenever the seal sits on a plate that will be thermally cycled or read in real time, not stored or incubated under simple lids. 
<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Optical-grade qPCR sealing films for fluorescence-based detection</li>
<li dir="ltr" role="presentation">Standard adhesive PCR films for routine thermal cycling</li>
<li dir="ltr" role="presentation">96-well microplate sealing films for standard PCR workflows</li>
<li dir="ltr" role="presentation">384-well compatible sealing films for high-throughput assays</li>
<li dir="ltr" role="presentation">High-temperature resistant films for -40&deg;C to 120&deg;C cycling</li>
<li dir="ltr" role="presentation">Low-evaporation sealing films for reaction stability</li>
<li dir="ltr" role="presentation">DNase/RNase-free films for contamination-sensitive assays</li>
</ul>
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<h2 dir="ltr">How to choose PCR or qPCR sealing film</h2>
 Confirm true optical clarity if you read fluorescence Not every "clear" sealing film has enough optical clarity and low enough autofluorescence for real-time PCR; choose a film specifically rated for qPCR with high light transmittance and no autofluorescence so excitation and emission pass cleanly. Standard storage films can suppress the signal. Decide between adhesive (PSA) and heat-seal Pressure-sensitive adhesive films are applied with a roller or paddle at the bench and peel off; heat-seal films bond under a plate sealer at controlled temperature and pressure for the strongest barrier in high-throughput and automated qPCR. PSA suits flexible workflows; heat-seal suits volume. Match the plate, thermal cycler, and skirt Confirm the film fits 96- or 384-well plates from your supplier and seats properly under the heated lid of your thermal cycler &mdash; full-skirt, semi-skirt, and non-skirt plates have different sealing surfaces. Sheet size near 137 &times; 80 mm is the SBS norm. Check chemical and temperature resistance PCR plate sealers and thermal cyclers reach 105 &deg;C routinely, and some films are rated to 150 &deg;C; check that the film and adhesive withstand the cycler protocol and the master mix without softening, lifting at corners, or leaving residue after peeling.
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<h2 dir="ltr">Specifications context</h2>
 Quality qPCR films are defined by light transmittance (typically &ge; 90 %), absence of autofluorescence, evaporation rate (commonly &le; 3 %), thermal tolerance to about 105 &deg;C with high-temperature variants to 150 &deg;C, and a DNase/RNase- and human-DNA-free certification appropriate for amplification. Most are supplied as flat SBS-format sheets around 137 &times; 80 mm, packed 100 per box; some have perforated end tabs for one-handed peeling. As of 2026, they sit in a low-to-mid price tier, with heat-seal films at the higher end.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a>&nbsp;to find the perfect optical or standard seal for your amplification workflow. Browse the wider&nbsp;<a href="https://mbpinc.net/consumables/sealing-films">sealing films range </a>for post-PCR archiving and reorder today via <a href="https://mbpinc.net/quickorder">Quick Order</a>.

PCR & qPCR Film

Product Literature

ThermalSeal® RT2 for Optically Clear for Flat-Top Plates

ThermalSeal® RT2 (TS-RT2-100) by MBP is an optically clear plate seal for qPCR and real-time fluorescence detection through the seal during thermocycling. Rated &minus;40&deg;C to +120&deg;C. Direct alternative to Bio-Rad MSB1001 Microseal. Available for flat-top (TS-RT2-100) and raised rim (TS-RT2RR-100) plates, 100 films/pack each. Ships ambient.

Application