Brands

<h2>What Is Reverse Transcriptase?</h2>
&nbsp;
Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Reverse Transcriptase</h2>
&nbsp;
Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

Reverse Transcriptase

DataSheet

MSDS

PCR Product Guide

OneScript Plus Reverse Transcriptase

G237 OneScript&reg; Plus Reverse Transcriptase by Applied Biological Materials is a thermostable (50&ndash;55&deg;C), RNase H-deficient M-MLV RT for cDNA synthesis up to 15 kb in 10&ndash;15 minutes, formulated with RNaseOFF inhibitor. Supplied as 100 rxn (100 µl enzyme + 400 µl 5X RT Buffer). ISO 13485:2016 MDSAP certified; store at &minus;20&deg;C.

Unit Quantity

Application

Storage Condition

Shipping Condition

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

OneScript Plus cDNA Synthesis Kit

G236 by Applied Biological Materials is the OneScript&reg; Plus cDNA Synthesis Kit (100 rxn) built on an engineered RNase H-null, thermally stable M-MLV RT. Synthesizes cDNA up to 12 kb at 50–55°C, with RNaseOFF inhibitor stable at &lt;1 mM DTT. Three primer options (oligo(dT), random, gene-specific). ISO 13485:2016 MDSAP Certified. Equivalent to G236EU. Store at &minus;20°C.

<h2>What are PCR Cleanup Kits?</h2>
&nbsp;
PCR cleanup kits remove residual PCR reaction components -- primers (&lt;100 bp), primer dimers, unincorporated dNTPs, DNA polymerase, buffer salts, and mineral oil - from PCR amplification products using silica-membrane spin columns and a bind-wash-elute protocol. The purified PCR amplicon (&gt;=100 bp) is recovered in 20-50 ul of water or elution buffer, free of inhibitors that would interfere with downstream steps, including Sanger sequencing, restriction enzyme digestion, ligation, NGS library preparation, and in vitro transcription. The procedure takes 5-10 minutes using a standard microcentrifuge with no organic solvents, precipitation, or centrifugation spins beyond the bind/wash/elute steps. Use a PCR cleanup kit immediately after PCR whenever the downstream application requires an inhibitor-free amplicon - primers and excess dNTPs left in the reaction will reduce sequencing quality, compete in ligation reactions, and inflate NGS background noise.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Oligo Clean &amp; Concentrator: Precisely designed systems for the quick retrieval of single-stranded DNA, RNA, and oligonucleotides from enzymatic processes.
</li>
<li dir="ltr" aria-level="1">
OneStep&trade; PCR Inhibitor Removal: Tailored columns and 96-well formats engineered to eliminate polyphenols, humic acids, and lipids that hinder PCR kinetics.
</li>
<li dir="ltr" aria-level="1">
ssDNA/RNA Clean &amp; Concentrator&trade;: An adaptable method for purifying and concentrating small nucleic acid segments, ensuring isoform specificity.
</li>
<li dir="ltr" aria-level="1">
ZR-96 High-Throughput Systems: Scalable formats of 96-well plates designed for automated lab processes, guaranteeing reproducibility-validated outcomes across extensive sample groups.
</li>
</ul>
&nbsp;
<h2>How to Choose a PCR Cleanup Kit</h2>
&nbsp;
Binding Capacity
Standard PCR cleanup columns bind up to 5-25 ug of DNA, depending on the kit; 25 ug capacity (Thermo Fisher GeneJET) accommodates typical preparative PCR reactions. For high-yield PCR reactions producing &gt;25 ug of amplicon, split the reaction across two columns and pool the eluates, or use a higher-capacity kit format.
Fragment Size Range
Standard PCR cleanup kits retain fragments &gt;=100 bp and discard everything shorter. For amplicons between 70 and 100 bp (e.g., short RT-qPCR products or miRNA amplicons), use a kit with an extended lower size limit -- the Thermo Fisher GeneJET kit retains fragments from 25 bp to 20 kb, covering this range. For amplicons shorter than 25 bp, conventional silica-column cleanup will not work; use gel purification or size-selective bead cleanup (e.g., AMPure XP at adjusted bead ratios).
Minimum Elution Volume
If the downstream application requires a high DNA concentration (Sanger sequencing needs &gt;20 ng/ul input), select a kit that elutes in as little as 20-25 ul without significant yield loss.&nbsp;
pH Indicator
Kits include a pH indicator dye in the binding buffer that turns yellow when the pH is correct for DNA binding and remains orange/red if the pH is too high (causing DNA loss). This is useful when purifying PCR products amplified in high-pH buffers. Kits without pH indicators require careful attention to binding buffer volume ratios to ensure efficient DNA adsorption.
96-Well Throughput
For screening workflows producing multiple amplicons per sample, use 96-well PCR purification plates with vacuum manifolds for simultaneous processing of all 96 wells. 96-well formats are essential for high-throughput genotyping and sequencing library preparation pipelines.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key benchmarks for PCR cleanup kits: &gt;=70% DNA recovery for fragments 100 bp-10 kb, A260/A280 &gt;= 1.7 in eluate, complete removal of primers and dNTPs (confirmed by capillary electrophoresis with no peaks below 100 bp), and elution in &lt;=50 ul of water or TE. The Thermo Scientific GeneJET PCR Purification Kit specifies recovery rates up to 100% with a binding capacity of 25 ug per column in a 5-minute protocol. AAT Bioquest study comparing commercial PCR cleanup kit performance confirmed that silica-column kits consistently achieve primer and dimer removal equivalent to ethanol precipitation with significantly reduced protocol time. PCR cleanup is a mandatory step before NGS library preparation in nearly all sequencing-based workflows at institutions, including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where primer and dimer contamination in libraries produces low-complexity sequencing reads that reduce effective coverage.
&nbsp;
Purify your amplicons and remove reaction noise&mdash;<a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> now to obtain a quote for your PCR cleanup process.

PCR Cleanup Kits

MSDS - D6035

MSDS - D6035-1-30

Protocol - D6035

OneStep-96™ PCR Inhibitor Removal Kit

OneStep-96™ PCR Inhibitor Removal Kit (D6035) by Zymo Research — 96-well format, 192 preps total. Removes humic/fulvic acids, polyphenols, tannins, and melanin from DNA and RNA extracts in a single step with no centrifugation. Prep Solution also sold separately (D6035-1-30).

Applicable For

Elution Volume

Equipment

Purity

Sample Source

Sample Storage

Type

Yield

Manufacturer

OneStep-96™ PCR Inhibitor Removal Kit (2 x 96 Preps.)

Prep Solution (30 ml)

MSDS - D6030

MSDS - D6031

Protocol - D6030

Protocol - D6031

OneStep™ PCR Inhibitor Removal Kit

The OneStep PCR Inhibitor Removal Kit by Zymo Research is a spin-column PCR inhibitor removal kit that clears humic/fulvic acids, tannins, polyphenols, and melanin from DNA and RNA in ~1 minute, 50–90% recovery, 50–200 µl input/elution. Available as the standard kit (D6030, 50 preps) or updated formulation (D6031, 50 preps).

OneStep™ PCR Inhibitor Removal Kit (50 Preps.)

OneStep™ PCR Inhibitor Removal Kit (Updated) (50 Preps.)

Serological Pipettes

<h2 dir="ltr"> What Are Serological Pipettes?</h2>
 Serological pipettes are calibrated, graduated, sterile polystyrene disposable pipettes used with a motorised pipette controller to transfer measured volumes of liquid in the 1&ndash;100 mL range. They are the primary tool for cell culture media transfer, buffer preparation, reagent dispensing in volumes above 1 mL, and serial dilutions requiring volumetric accuracy in the millilitre range. They differ from aspirating pipettes &mdash; which have no graduations and are used only for vacuum-assisted waste removal &mdash; and from microcentrifuge tubes, which are not dispensing instruments. Serological pipettes are the correct format for any measured liquid transfer &ge;1 mL in cell culture or reagent preparation; pair them with a motorised pipette controller for safe, ergonomic operation. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">1 mL to 50 mL Serological Pipettes &ndash; Standard range for routine and high-volume liquid transfer</li>
<li dir="ltr" role="presentation">Glass Serological Pipettes &ndash; Reusable options for solvent resistance and durability</li>
<li dir="ltr" role="presentation">Sterile Serological Pipettes &ndash; Gamma-sterilized for aseptic laboratory use</li>
<li dir="ltr" role="presentation">Non-Sterile Serological Pipettes &ndash; Cost-effective option for general applications</li>
<li dir="ltr" role="presentation">Graduated Serological Pipettes &ndash; For accurate volume measurement and dispensing </li>
</ul>
&nbsp;
<h2>How to Choose Serological Pipettes</h2>
 Volume: Standard Sizes and ISO 7550 Colour Coding ISO 7550 assigns a specific colour code to each serological pipette volume: 1 mL = red; 2 mL = yellow; 5 mL = blue; 10 mL = orange; 25 mL = green; 50 mL = purple (pink/violet depending on supplier). Colour coding allows quick visual volume identification when multiple sizes are in use simultaneously in a BSC. Packaging: Individual Wrap vs Bulk Individually wrapped sterile pipettes are required for cell culture, molecular biology, and aseptic technique. Plastic/plastic peel-open packaging eliminates paper particulate from paper/plastic wrappers &mdash; important in sensitive cell culture applications and for RNA work. Bulk-packed (canister or bag) formats are cost-effective for non-sterile applications such as buffer preparation and reagent dispensing in fume hoods. Construction: 1-Piece vs 3-Piece 1 mL, 2 mL, and 5 mL serological pipettes are typically one-piece moulded polystyrene constructions. 10 mL, 25 mL, and 50 mL pipettes use 3-piece ultrasonically welded construction (Globe Scientific) for the body-to-cone and body-to-cap joints &mdash; essential for the structural integrity of larger formats under aspiration vacuum. Tip and Graduation Features Negative graduations (markings above the nominal maximum) allow volumes slightly above nominal to be read. Fine-point tips with slow drip-off improve pipetting accuracy during cell culture media transfers. Some brands offer Wobble-not-style pipettes with reduced barrel flex for easier single-hand operation. Filter Plug Material Cotton filter plugs are standard; synthetic plugs (polyethylene or polypropylene) are preferred when processing organic solvents or any media that might wick through a cotton plug.
<h2 dir="ltr"> Compare Serological Pipette Volumes </h2>
&nbsp;
<ul>
<li>1 mL &ndash; Red ISO 7550 colour, 1-piece construction, 0.01 mL graduations; used for small-volume media transfer.</li>
<li>2 mL &ndash; Yellow ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for buffer preparation.</li>
<li>5 mL &ndash; Blue ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for media preparation and dilutions.</li>
<li>10 mL &ndash; Orange ISO 7550 colour, 3-piece construction, 0.1 mL graduations; used for flask media changes.</li>
<li>25 mL &ndash; Green ISO 7550 colour, 3-piece construction, 0.5 mL graduations; used for bulk media dispensing.</li>
<li>50 mL &ndash; Purple ISO 7550 colour, 3-piece construction, 1.0 mL graduations; used for large flask media changes.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 Serological pipettes are manufactured from optically clear virgin polystyrene in Class 100,000 cleanroom conditions. Sterility: individually wrapped pipettes are EO-sterilised to SAL 10⁻⁶; non-pyrogenic, non-cytotoxic, DNase-free, and RNase-free per lot. ISO 7550 defines colour coding for serological pipettes; many US suppliers follow this standard, though it is not universally mandated. Calibrated to deliver (TD) with blow-out (marked with a double band or 'TD' marking) &mdash; all liquid must be expelled from the tip for accurate delivery. Globe Scientific, Nest Scientific, and Falcon remain the dominant serological pipette brands in North American cell culture labs.&nbsp; 
&nbsp;
Improve consistency and reduce handling variation with reliable serological pipette solutions. Request a quote from <a href="https://mbpinc.net/contact-us">the expert team at Molecular Biology Products Inc. (MBP).</a>

1864-Group-Serological Pipette Brochure

Open Ended Serological Pipettes

The Open Ended Serological Pipettes by Globe Scientific are gamma-sterilized PS pipettes with open (unplugged) tips for high-viscosity liquid dispensing. Three volumes: 1864 (2 mL, green, 270 mm, 800/box), 1868 (5 mL, blue, 310 mm, 300/box), 1872 (10 mL, red, 310 mm, 200/box). Individually wrapped, paper/plastic.

Manufacture

Serological Pipette, 2mL, PS, Open End, 270mm, STERILE, Green, Individually Wrapped, Paper/Plastic, BOX/800

Serological Pipette, 5mL, PS, Open End, 310mm, STERILE, Blue, Individually Wrapped, Paper/Plastic, BOX/300

Serological Pipette, 10mL, PS, Open End, 310mm, STERILE, Red, Individually Wrapped, Paper/Plastic, BOX/200

<h2>What are prestained protein ladders?</h2>
&nbsp;
Prestained protein ladders are protein molecular weight standards in which each protein band carries a covalent colored dye, typically coupling most bands in blue with two specifically colored bands, often green around 25 kDa and red around 75 kDa, that serve as easily visible reference anchors for quick orientation. Because the dye is visible without staining, the ladder is used to monitor protein migration in real time during SDS-PAGE and, critically, to verify that protein bands have transferred from the gel to the PVDF, nitrocellulose, or nylon membrane before antibody incubation in western blotting.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">PureView&trade; Prestained Protein Ladder for clear visualization of&nbsp; bands in SDS-PAGE</li>
<li dir="ltr" role="presentation">SmartCheck&trade; Tri-color Extended Range Plus Protein Marker for broad coverage of protein size&nbsp;</li>
<li dir="ltr" role="presentation">Opti-Protein Marker for accurate molecular weight estimation of proteins</li>
<li dir="ltr" role="presentation">Opti-Protein XL Marker for wider range analysis of proteins&nbsp;</li>
<li dir="ltr" role="presentation">Opti-Protein Ultra Marker for efficient protein separation workflows</li>
</ul>
&nbsp;
<h2>How to use prestained protein ladders</h2>
&nbsp;
Load prestained ladders directly from the tube without preparation
Most modern prestained protein ladders are supplied in gel loading buffer and are ready to load directly without heating, dilution, or addition of reducing agent, since modifying the formulation can cause dye dissociation or protein aggregation. Following the manufacturer's specific note about preparation avoids introducing variability.
Confirm the ladder's kDa range brackets your target protein's expected molecular weight
Match your target protein's expected size to the ladder's coverage: a mid-range ladder (10&ndash;180 kDa) suits most antibody targets in typical western blot experiments, while a broad-range (10&ndash;245 kDa) or extra-broad (3.5&ndash;260 kDa) ladder is needed when the target protein is very large.
Use the colored reference bands for quick gel and blot orientation
Colored reference bands at fixed kDa positions, typically green at 25 kDa and red at 75 kDa, allow you to orient the ladder pattern on the gel or membrane at a glance without counting all bands from the well, making it fast to identify the bands bracketing your target protein.
Check the manufacturer's MW table calibrated for your specific SDS-PAGE buffer system
Pre-stained ladder proteins have apparent molecular weights that differ depending on the SDS-PAGE buffer system used, such as Tris-Glycine versus Bis-Tris MOPS or MES, because the buffer's pH and composition affect SDS binding and protein charge. Using the manufacturer's annotated molecular weight values calibrated for your specific buffer gives more accurate sizing than a generic table.
Verify western blot transfer by confirming the prestained bands are visible on the membrane
After transfer, all or most prestained ladder bands should be visible on the blot membrane; missing or faint bands in a particular region indicate incomplete transfer in that molecular weight zone, guiding troubleshooting before antibody incubation and detection.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Prestained protein ladders are loaded at approximately 3&ndash;5 &micro;l per lane for gel visualization during SDS-PAGE, or at 1.5&ndash;2.5 &micro;l per lane for western blot transfer, since less ladder per lane is needed for adequate band visibility on the membrane than for gel visualization. As of 2026, ready-to-load prestained protein ladder formats supplied pre-formulated in SDS-PAGE loading buffer that require no heating, dilution, or reducing agent remain the dominant format for routine SDS-PAGE and western blotting, since preparation-free direct loading eliminates one class of user handling errors.
<a href="https://mbpinc.net/contact-us">Contact the helpful team at Molecular Biology Products Inc. (MBP)</a> and find the best prestained protein ladders for your next experiment.

Prestained Protein Ladders

Protein Markers

Opti-Protein Marker

Opti-Protein Marker G252 (ABM) — pre-stained protein molecular weight standard for SDS-PAGE and Western blot. Ready to load (5–10 µl/lane). Visible during electrophoresis for real-time migration monitoring and transfer verification. Store at −20 °C.

Shipping condition

Opti-Protein Ultra Marker

Opti-Protein Ultra Marker (G623) by Applied Biological Materials is a ready-to-use pre-stained protein ladder (10 bands, 6.5–270 kDa, 500 µl/100 loads) with three color-coded reference bands (orange at 270 &amp; 30 kDa, green at 52 kDa) for SDS-PAGE sizing and Western blot transfer monitoring. Load 5 µl. Store at 4°C (3 months) or –20°C (24 months). Updated version of G252.

Opti-Protein XL Marker

Opti-Protein XL Marker G266 (ABM) — pre-stained high-molecular-weight protein ladder for SDS-PAGE and Western blot. Ready to load (5–10 µl/lane). Visible during electrophoresis for real-time migration monitoring of large proteins. Store at −20 °C.

PCR & qPCR Film

Opti-Seal qPCR and Storage Films

Opti-Seal qPCR and Storage Films G495 — 50 µm polyolefin with encapsulated silicone adhesive. Optically clear, low auto-fluorescence, heat-resistant seal. Fits raised-rim 96-well plates (78.6 × 139.7 mm). 100 films/box. For qPCR, sample storage, and protein crystallization.

Optical Sealing Films

Optical Sealing Films are available in the following variants: SF-UC-500, SF-UC-550.SF-UC Optical Sealing Films by Novasbio are ultra-clear polyolefin qPCR plate seals with non-fluorescing pressure-sensitive adhesive, rated −80°C to +105°C and certified DNase/RNase/human-DNA-free. Universal format fits flat-top, non-skirted, and semi-skirted 96-well plates. Available from MBP Inc.

Packaging

Applications

QPCR Optical Seal, Ultra Clear, pressure sensitive adhesive for real-time PCR 100/PK 5PK/CS

PCR, QPCR Optical Seal, strong adhesive for real-time PCR and PCR 100/PK 5PK/CS

Plate Sealer Accessories

<h2>What Are Plate Sealer Accessories?</h2>
&nbsp;
Plate sealer accessories include consumable sealing materials and instrument hardware used with heat plate sealers. Consumable sealing materials, such as sealing films and foil seals, are the most frequently replaced accessories, with one seal used per plate. Films and foils vary in optical properties, adhesion, temperature resistance, and pierceability, making it important to select the appropriate type for PCR, qPCR, sample storage, or automated liquid handling. Instrument hardware accessories, such as sealing frames, adapter inserts, and replacement components, are model-specific and designed to maintain proper sealer performance. Before ordering, confirm whether your application requires an optically clear film, a pierceable foil, or a cryogenic-rated foil.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Optically clear qPCR sealing films with peelable, pierceable, and strong-bond options.</li>
<li>Peelable sealing films compatible with most microplate types for PCR and qPCR workflows.</li>
<li>Strong-bond sealing films for polypropylene (PP) plates requiring secure sample containment.</li>
<li>Foil plate seals with peelable or strong-bond options for PP plates.</li>
<li>Foil seals suitable for DMSO applications and cold storage from -200&deg;C to 110&deg;C.</li>
</ul>
&nbsp;
<h2>How to Choose Plate Sealer Accessories</h2>
&nbsp;
Seal material: film vs. foil
Polymer films are optically transparent and required for qPCR, fluorescence assays, and plate readers that detect through the seal. They are suitable for thermal cycling and short-term storage. Aluminum foil seals provide superior evaporation protection and are preferred for long-term storage, solvent resistance, and applications that do not require optical read-through.
Optical properties for qPCR
Use optically clear, low-autofluorescence sealing films for real-time qPCR to maximize fluorescence detection sensitivity. Confirm compatibility with your PCR instrument and fluorescence detection wavelengths.
Cryogenic performance
For storage at -80&deg;C or below, select sealing materials designed to maintain adhesion and barrier integrity through repeated freeze-thaw cycles. Cryogenic-rated foil seals provide the most reliable long-term performance.
Pierceable vs. non-pierceable seals
Pierceable foil seals allow robotic or manual pipette access without removing the seal, making them suitable for automated workflows. Non-pierceable seals provide the highest barrier protection for storage but must be removed before accessing samples.
Format adapters and sealing frames
Sealing frames and adapter inserts help align sealing materials and non-standard microplate formats during heat sealing. Verify compatibility with both your plate sealer model and microplate format before ordering.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Film thickness influences evaporation protection, optical clarity, and piercing performance. Polymer films are typically 60 to 100 micrometers thick, while aluminum foils are generally 50 to 70 micrometers thick. Also confirm whether your plate sealer uses pre-cut sealing sheets or roll-format sealing material, as these formats are not interchangeable.
&nbsp;
To discuss product availability or get selection guidance,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Optically clear sealing film for qPCR, Peelable, for most plate types, 100/pk

The MS1000-PCR1 by Benchmark Scientific is an optically clear, peelable qPCR sealing film supplied as 125 × 78 mm sheets (100/pack). Compatible with PP, PC, and PE 96-well plates, it maintains fluorescence-grade optical clarity across –110°C to 200°C and allows post-run sample access via its peelable adhesive—designed for use with the Benchmark MS1000 SmartSeal sealer and most standard heat sealers.

Optically clear sealing film for qPCR, Pierceable, for most plate types, 100/pk

MS1000-PCR3 by Benchmark Scientific is an optically clear, pierceable qPCR sealing film (125 mm × 78 mm) rated from −200°C to +110°C, compatible with PP, PC, and PE microplates. Each pack contains 100 sheets. Designed for use with the Accuris SmartSeal sealer (MS1000) and most standard thermal plate sealers, it keeps plates sealed during thermal cycling while allowing robotic tip piercing between workflow steps.