Brands

<h2 dir="ltr">What Are Luciferase Reporter Lentiviral Vectors?</h2>
 Luciferase reporters have been foundational to gene expression research since their first use as reporter genes in 1985. The bioluminescent signal produced when luciferase oxidises its substrate provides a sensitive, quantitative, non-radioactive readout measurable over a wide dynamic range with standard plate reader luminometers. Lentiviral delivery produces stable, integrated reporter cell lines that maintain consistent signal over weeks to months. Accurate reporter assays depend on appropriate detection systems. Select a luciferase construct that fits your research needs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Luciferase Reporters: Reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background controls with <a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">luciferase reporter systems</a>.</li>
<li>Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li>Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li>Promoter &amp; UTR Reporter Systems: Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li>Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
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<h2 dir="ltr"> How to Choose a Luciferase Reporter</h2>
 Reporter Role Firefly luciferase is typically driven by the promoter or response element under investigation as the experimental reporter; Renilla luciferase &mdash; driven by a constitutive promoter (SV40 or CMV) &mdash; provides within-sample normalisation correcting for cell number, transfection efficiency, and lysis variability. Substrate and Emission Firefly luciferase uses D-luciferin and emits at ~550&ndash;570 nm (green/yellow); Renilla uses coelenterazine and emits at ~480 nm (blue-green) &mdash; both can be measured sequentially from the same lysate in a dual-luciferase assay. Stable vs. Transient Use Lentiviral delivery produces stable reporter cell lines in 1&ndash;2 weeks via antibiotic selection &mdash; essential for high-throughput compound screening, long-term pathway activity monitoring, and in vivo bioluminescence imaging. Pathway-Specific Reporters Promoter or transcriptional response element (TRE) inserts &mdash; such as Wnt/TCF-LEF, NF-&kappa;B, NFAT, or VEGF promoter &mdash; provide a quantitative readout of pathway activity when the cognate transcription factor is activated. In Vivo Imaging Needs CMV-GFP-T2A-Luciferase dual-reporter lentivectors co-express GFP and luciferase for combined fluorescence and bioluminescence tracking of implanted cells in live animals. 
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<h2 dir="ltr">Specifications Context</h2>
 Cells are lysed and mixed with the appropriate substrate in a plate reader or tube luminometer; light emission is measured in relative light units (RLU), proportional to luciferase activity and reflecting promoter activity or transgene expression level. A promoter-less dual luciferase vector establishes the minimum signal level for the assay system, identifying the true zero-activity baseline. Stable lentiviral luciferase reporter cell lines are essential tools for tracking xenograft tumour growth, metastasis, and therapy response in preclinical mouse models. EF1&alpha;-driven constructs maintain high expression for 30+ days without selection, while CMV-driven constructs may undergo progressive silencing in some cell types. Product availability reflects MBP's catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get optimized luciferase reporters for advanced cloning and CRISPR-related workflows.

Luciferase Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Luciferase (Luciola italica) Lentivirus (minCMV)(Bicistronic)

LVP010137 by Applied Biological Materials is a pre-packaged bicistronic lentivirus expressing Luciola italica luciferase under a minimal CMV promoter, with a PGK-driven MCS in the opposite orientation. Supplied at 107 IU/ml as 3 × 250 µl aliquots (Dry Ice). For stable luciferase reporter cell lines, bioluminescence imaging, and in vivo cell tracking. Store at &minus;80°C; do not refreeze thawed aliquots.

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Luciferase (Photinus pyralis) Lentiviral Vector (Bicistronic)(minCMV)

LV010146 by Applied Biological Materials is a 1.0 µg bicistronic lentiviral plasmid vector carrying Photinus pyralis Luc2 under a minimal CMV promoter and a PGK-driven MCS in the opposite orientation (GenBank EU921841). Designed for luciferase reporter studies and dual-expression lentiviral experiments. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Luciferase (Photinus pyralis) Lentiviral Vector (UbC)

LV010087 by Applied Biological Materials is a 1.0 µg lentiviral plasmid vector carrying Photinus pyralis Luc2 luciferase (EU921841) under a UbC promoter, with Kanamycin (bacterial) and Puromycin (mammalian) resistance. For BLI reporter and stable cell line applications. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Luciferase (Photinus pyralis) Lentivirus (Bicistronic)(minCMV)

LVP010146 by Applied Biological Materials is a pre-packaged bicistronic Photinus pyralis Luc2 (minCMV) / PGK-MCS bidirectional lentiviral virus. 107 IU/mL, 3 × 250 µL. D-luciferin substrate BLI. PGK-MCS for second-gene cloning (plasmid version). Plasmid: LV010146. Store at −80°C; shipped on dry ice.

Full Sequence of LV010141

QC - Lot. No.0101

Luciferase (Photinus pyralis) RFP Lentiviral Vector (Bicistronic)(minCMV)(PGK)

LV010141 by Applied Biological Materials is a 1.0 µg bicistronic bidirectional lentiviral vector co-expressing Photinus pyralis Luc2 luciferase under minCMV and RFP under PGK. Dual-reporter construct for simultaneous BLI and fluorescence cell tracking. Requires lentiviral packaging. Store at −20°C.

Luciferase (Photinus pyralis) RFP Lentivirus (Bicistronic)(minCMV)(PGK)

LVP010141 by Applied Biological Materials is a pre-packaged bicistronic Photinus pyralis Luc2 (minCMV, D-luciferin BLI) / RFP (PGK) bidirectional lentiviral virus. 107 IU/mL, 3 × 250 µL. Dual BLI + RFP tracking from a single integration. Plasmid: LV010141. Store at −80°C; shipped on dry ice.

Luciferase (Renilla reniformis) Lentiviral Vector (CMV)

LV010079 by Applied Biological Materials is a 1.0 µg lentiviral plasmid vector carrying Renilla reniformis luciferase under a CMV promoter, with Kanamycin (bacterial) and Puromycin (mammalian) resistance. For dual-luciferase assays and stable Renilla reporter cell lines. Coelenterazine substrate. Requires lentiviral packaging. Store at −20°C, non-frost-free, up to 1 year.

Storage Condition

Luciferase (Renilla reniformis) Lentivirus (CMV)

LVP010079 by Applied Biological Materials is a pre-packaged Renilla reniformis luciferase (CMV) lentiviral virus. Supplied at 107 IU/mL as 3 × 250 µL. Kanamycin (bacterial) + Puromycin (mammalian) selection. Coelenterazine substrate BLI. Ready to transduce. Plasmid: LV010079. Store at −80°C; shipped on dry ice.

Luciferase (Renilla reniformis) RFP Lentiviral Vector (Bicistronic)(minCMV)(PGK)

LV010081 by Applied Biological Materials is a 1.0 µg bicistronic lentiviral vector co-expressing Renilla reniformis luciferase (RenLuc, AAO48589) under minCMV and RFP under PGK. Dual Renilla BLI (coelenterazine) and red fluorescence tracking. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Luciferase (Renilla reniformis) RFP Lentivirus (Bicistronic)(minCMV)(PGK)

LVP010081 by Applied Biological Materials is a pre-packaged bicistronic Renilla reniformis luciferase (minCMV) / RFP (PGK) lentiviral virus. Supplied at 107 IU/mL as 3 × 250 µL. Dual Renilla BLI (coelenterazine) + RFP tracking. Ready to transduce. Plasmid: LV010081. Store at −80°C; shipped on dry ice.

<h2>What are Bioluminescence Assays?</h2>
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Bioluminescence assays use luciferase enzymes to convert specific substrates into light, producing a highly sensitive readout for gene expression studies or cell viability measurements. In ATP-based systems, light output correlates with cellular ATP levels and therefore cell viability.
Firefly luciferase uses luciferin along with ATP, magnesium, and oxygen to generate a green/yellow signal with a decay time of roughly 12 minutes. Renilla luciferase uses coelenterazine and oxygen, producing a faster-decaying blue signal and not requiring ATP.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Luciferase Assay Systems: High-performance kits like the Luciferase Assay Kit (G287), designed for rapid, flash-type bioluminescence detection that provides a direct and sensitive measure of gene induction.
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Fluorescent Control Proteins: Premium GFP Purified Protein (000033P) available as a reliable internal standard or calibration marker to validate transfection efficiency and protein localization across diverse imaging platforms.
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<h2>How to Choose a Bioluminescence Assay System</h2>
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Decide Between Single and Dual-Reporter Designs
Dual-reporter systems combine a test luciferase with a constitutively expressed control luciferase, allowing normalization for variability in transfection efficiency, cell number, and viability.
Choose NanoLuc for Maximum Sensitivity
NanoLuc is an engineered 19 kDa luciferase that produces approximately 100&ndash;150&times; brighter signal than firefly or Renilla systems and uses furimazine as its substrate.
Match Substrate and Cofactor Requirements
Firefly luciferase requires luciferin, ATP, Mg2+, and oxygen, while Renilla luciferase requires coelenterazine and oxygen only, which is important when ATP levels are part of the experimental variable.
Consider Destabilized Reporters for Faster Response
Destabilized luciferase variants have shorter intracellular half-lives, allowing more rapid detection of transcriptional changes with reduced signal lag.
Use ATP-Based Assays for Viability
ATP-based bioluminescence assays measure cellular viability rather than gene expression, as light output reflects ATP levels in metabolically active cells.
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<h2>Specifications Context</h2>
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The Dual-Luciferase Reporter system combines firefly and Renilla luciferase to allow sequential measurement using a luciferase inhibitor for normalization of experimental variability.
As of 2026, NanoLuc-based systems are increasingly used beyond traditional reporter gene assays, including protein interaction and target engagement studies due to their high brightness and low background.
Enhance your signal detection&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional bioluminescence solutions.

Bioluminescence Assays and Reagents

Luc Gene Test with G287 Report-2

Luciferase Assay Kit

Luciferase Assay Kit (G287) by ABM delivers 500 reactions for firefly luciferase reporter gene quantification in mammalian and bacterial cell cultures, with a two-reagent workflow and luminescence readout within 30 minutes.

Usage

Sterility

LV010138 Insert Sequence

Luciferase-mCherry Lentiviral Vector (CMV)

LV010138 by Applied Biological Materials is a 1.0 µg dual-reporter lentiviral vector co-expressing mCherry (CMV, constitutive) and Photinus pyralis Luc2 (3×HRE-SV40, hypoxia-inducible). With Kanamycin and Puromycin resistance. For hypoxia BLI and fluorescence tracking studies. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.