Brands

<h2>What are bisulfite conversion reagents and buffers?</h2>
&nbsp;
Bisulfite conversion reagents include sodium bisulfite solution, denaturation and conversion buffers, and complete kits that bundle all components needed for the three-step chemical reaction: sulfonation of cytosine to a cytosine-bisulfite derivative, hydrolytic deamination to a uracil-bisulfite derivative, and alkali desulfonation to yield uracil. This process converts unmethylated cytosines to uracil while leaving 5-methylcytosine intact and unconverted, because 5-methylcytosine resists bisulfite-mediated deamination under standard conditions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">RNA and DNA conversion reagents for enhanced modification of cytosine in methylation workflows</li>
<li dir="ltr" role="presentation">Desulphonation buffers for stabilization of nucleic acids and post-conversion cleanup&nbsp;</li>
<li dir="ltr" role="presentation">Binding buffers used during purification for efficient capture of converted RNA and DNA</li>
<li dir="ltr" role="presentation">Wash buffers for the removal of inhibitors and improved purity of samples</li>
<li dir="ltr" role="presentation">Elution buffers for high-quality recovery of converted nucleic acids</li>
<li dir="ltr" role="presentation">DNA denaturing and digestion buffers for controlled workflow and optimized conversion efficiency</li>
<li dir="ltr" role="presentation">Dilution and reaction buffers for maintaining stable assay conditions</li>
<li dir="ltr" role="presentation">Solubilization and dissolution buffers for sample processing and preparation&nbsp;</li>
<li dir="ltr" role="presentation">Lightning conversion reagents for efficient and rapid methylation analysis workflows</li>
</ul>
&nbsp;
<h2>How to use bisulfite conversion reagents</h2>
&nbsp;
Ensure complete DNA denaturation before conversion to avoid incomplete conversion
Bisulfite only acts on cytosines in single-stranded DNA, so complete denaturation of the input DNA is the most critical first step, since failure to fully denature can leave double-stranded regions in which cytosines are shielded from the bisulfite reagent and remain unconverted.
Understand that incomplete conversion produces false-positive methylation results
If the conversion is incomplete, unconverted unmethylated cytosines will read as cytosine after sequencing, which will be incorrectly interpreted as methylated cytosine, artificially inflating the apparent methylation level of the sample in a false-positive direction.
Optimize conversion conditions to balance completeness against DNA degradation
The harsh conditions needed for complete bisulfite conversion, including high bisulfite concentration, elevated temperature, and long incubation, also fragment and degrade DNA, and optimizing conditions that achieve high conversion while minimizing degradation is a key technical challenge in bisulfite workflows.
Include an unmethylated conversion control to estimate residual incomplete conversion
Including an unmethylated control DNA, such as lambda phage DNA, in the same conversion reaction provides a readout of how completely unmethylated cytosines were converted, helping quantify residual incomplete conversion rates and account for them in downstream analysis.
Be aware of specific challenges for FFPE and degraded DNA samples
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before bisulfite treatment, and the additional fragmentation during conversion can result in poor library yields; specific modified protocols and carrier reagents can help reduce further degradation during the conversion step.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its high resolution and accuracy at single-base level, but it also introduces specific DNA quality challenges for downstream library preparation and sequencing, particularly for samples already of poor quality like FFPE tissue. As of 2026, enzymatic conversion is increasingly recommended as the preferred alternative to bisulfite conversion specifically because it achieves comparable discrimination between methylated and unmethylated cytosines while minimizing the DNA damage and library complexity loss that harsh bisulfite chemistry introduces.
This category sits within DNA/RNA Methylation under Epigenetics, and for a synthetic, fully methylated positive control to run through the conversion workflow, see dNTPs (Deoxynucleotide Triphosphates). See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and request a quote today to get high-quality bisulfite conversion reagents for your lab.

Bisulfite Conversion Reagents & Buffers

D5001-2-MSDS

M-Dilution Buffer

D5001-2 by Zymo Research is M-Dilution Buffer (1.3 mL) — a required component of the EZ DNA Methylation Kit used to prepare DNA samples for bisulfite conversion (5 µL per reaction) and to dissolve CT Conversion Reagent (210 µL per tube). Sold separately for component replenishment.

Manufacturer

Shipping Condition

Storage Condition

M-Dilution Buffer (1.3 ml)

M-Dilution Buffer (5.2 ml)

D5005-2-MSDS

M-Dilution Buffer-Gold

The D5005-2 by Zymo Research is M-Dilution Buffer-Gold (1.5 ml), used exclusively to prepare the CT Conversion Reagent in the EZ DNA Methylation-Gold Kit for bisulfite conversion of genomic DNA. Sold as a standalone replacement when this component is depleted before other kit reagents. Made in the USA; not interchangeable with M-Dilution Buffer from the standard EZ DNA Methylation kit.

M-Dilution Buffer-Gold (1.5 ml)

M-Dilution Buffer-Gold (7 ml)

D5005-6-MSDS

M-Dissolving Buffer

D5005-6 by Zymo Research is a 500 µl standalone M-Dissolving Buffer for preparing the CT Conversion Reagent in the EZ DNA Methylation-Gold Kit (D5005/D5006). It dissolves the lyophilized reagent into a homogeneous working solution and is available separately for component replenishment. Stored at room temperature. Incompatible with EZ DNA Methylation Kit (D5001), which uses a different buffer formulation.

M-Dissolving Buffer (500 µl)

M-Dissolving Buffer (1.2 ml)

D5001-6 - MSDS

M-Elution Buffer

D5001-6 M-Elution Buffer by Zymo Research — 1 ml; dedicated elution buffer for bisulfite-converted DNA; compatible with EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits.

M-Elution Buffer (1.0 ml)

M-Elution Buffer (4 ml)

M-Elution Buffer (8 ml)

M-Elution Buffer (40 ml)

MSDS

M-Elution Buffer (50 ml)

D5049-6 by Zymo Research is M-Elution Buffer (50 mL) for eluting bisulfite-converted DNA from Zymo-Spin columns in EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning Kit workflows — 10 µL per column elution. Eluate is ready for immediate use or storage at ≤–20°C.

M-Reaction Buffer

D5020-8 M-Reaction Buffer by Zymo Research is a 1 ml replacement component for the EZ DNA Methylation-Direct Kit, stored at room temperature. Used at 160 µl per CT Conversion Reagent preparation in bisulfite conversion workflows.

M-Reaction Buffer (1 ml)

M-Reaction Buffer (4 ml)

M-Solubilization Buffer

M-Solubilization Buffer (D5020-7) by Zymo Research — 4.5 ml reagent for dissolving the CT Conversion Reagent in the EZ DNA Methylation-Direct Kit. Stored at room temperature; sold separately or as a kit component. Made in the USA.

M-Solubilization Buffer (4.5 ml)

M-Solubilization Buffer (18 ml)

D5001-4 - MSDS

M-Wash Buffer

D5001-4 by Zymo Research is a 6 mL M-Wash Buffer used in the wash step of bisulfite-converted DNA purification across the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kit series.

M-Wash Buffer (6 ml)

M-Wash Buffer (24 ml)

M-Wash Buffer (72 ml)

M-Wash Buffer (36 ml)

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

M13 Isolation Kit

M13 Isolation Kit (PD1316-00) by MBP is a spin-column kit for isolating high-purity M13 bacteriophage single-stranded DNA from bacterial cultures. Kit includes DNA mini columns, collection tubes, and all required buffers. Available in 4 (PD1316-00), 50 (PD1316-01), and 250 (PD1316-02) prep sizes. Ships ambient.

M13 Isolation Kit , 4 Preps

M13 Isolation Kit , 50 Preps

M13 Isolation Kit , 250 Preps

<h2 data-section-id="185nm5c" data-start="0" data-end="38">What is classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture for decades, including DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's F-10, Ham's F-12, IMDM (Iscove's Modified Dulbecco's Medium), and DMEM/F-12. These media provide the amino acids, vitamins, glucose, salts, buffering agents, and other nutrients required to support cell survival and growth in vitro. They form the basis of many modern specialized, serum-free, and advanced media formulations used in academic research, biotechnology, pharmaceutical development, and biomanufacturing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
&nbsp;Availability of both low and high carbohydrate levels of balanced basal formulations for the distinct metabolic requirements of diverse mammalian cell lines.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Maintenance of precise physiological pH during long-term incubation cycles using optimized buffering configurations with integrated chemical stabilizers&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Nutrient-dense cell media supplemented with vitamins and essential amino acids to support&nbsp; hybridomas and rapidly proliferating continuous cultures
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Optimized serum reduction along with enhanced cell attachment using&nbsp;Multi-purpose 1:1 formulation hybrids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Salt solutions supplemented with essential minerals to facilitate enzyme preparation, cell washing, and reliable short-term osmotic maintenance.
</li>
</ul>
&nbsp;
<h2 data-section-id="1blio5n" data-start="931" data-end="964">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acids and vitamins found in MEM, along with higher glucose concentrations, making it suitable for cell types with elevated nutrient demands. It is widely used for primary cells, stem cells, and many transformed mammalian cell lines. MEM, based on the original Eagle's formulation, remains a common choice for standard monolayer culture and is available with different salt formulations to support various applications.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640 was originally developed for culturing human leukemic cells and remains the preferred medium for lymphocytes, hybridomas, and other hematopoietic cell types. Its formulation includes glutathione and elevated levels of biotin, vitamin B12, and PABA. Although designed for suspension cultures, RPMI 1640 also supports a wide range of adherent cell lines when appropriately supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were developed for CHO cells and other specialized cell types. Their formulations include additional nutrients such as zinc, putrescine, hypoxanthine, and thymidine, making them useful for hepatocyte culture, toxicity testing, viral fusion studies, and other specialized applications. These media can be used with or without serum depending on the experimental requirements.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 combines the nutrient-rich composition of DMEM with the specialized components found in Ham's F-12. The resulting formulation supports demanding cell types including MDCK cells, glial cells, fibroblasts, and endothelial cells. It is also widely used as the foundation for many serum-free and advanced culture systems.
IMDM for high-density rapid proliferation
IMDM is an enriched derivative of DMEM that contains additional amino acids, vitamins, HEPES buffer, sodium pyruvate, and potassium nitrate. Its enhanced nutrient profile makes it particularly suitable for rapidly proliferating and high-density cultures such as Jurkat cells, COS-7 cells, and macrophages.
Liquid vs powder format
Liquid classic media are supplied ready for use after the addition of serum and required supplements. Powdered media offer lower shipping costs and greater flexibility for high-volume laboratories but require reconstitution, sodium bicarbonate addition, and sterile filtration before use.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4382" data-end="4407">Specifications context</h2>
&nbsp;
Classic media are manufactured according to their original published formulations, with only stability-related modifications where necessary. Most liquid formulations contain sodium bicarbonate as the primary buffering system and require incubation in a 5&ndash;10% CO₂ atmosphere to maintain physiological pH. Powdered formulations require reconstitution, sodium bicarbonate supplementation, and sterile filtration before use.
As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the most widely used classic cell culture media across academic, biotechnology, pharmaceutical, and biomanufacturing laboratories. IMDM continues to gain popularity for high-density culture and bioprocessing applications.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents such as GlutaMAX, NEAA, sodium pyruvate, and HEPES, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized classic media for your lab.

Classic Media

M199 Medium

M199 Medium (TM502) by Applied Biological Materials is a 500 ml sterile basal medium containing L-glutamine, sodium bicarbonate (2.2 g/L), and phenol red (no HEPES) for broad-species primary cell culture, virology, and vaccine production. Supplement as required; requires 5–10% CO₂. Store at 2–8°C, protected from light.

Unit Quantity

Sterility

Appearance

Disclaimer

Caution

Orbital Shaker Accessories

<h2>What are orbital shaker accessories?</h2>
&nbsp;
Orbital shaker accessories are the platform attachments and holders that secure specific vessel types to a shaker platform during operation. Erlenmeyer flasks typically require spring-clip clamps that hold the flask securely while allowing unrestricted liquid movement, microplates use dedicated plate holders, tube rack inserts accommodate centrifuge and microcentrifuge tubes, and non-slip mats provide flexible placement for vessels during lower-speed mixing applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>
Dedicated Flask Platforms: Pre-configured platforms designed to securely hold specific sizes of Erlenmeyer flasks or standard microplates.
</li>
<li>
Universal Magnetic Platforms: Versatile, metallic base platforms engineered for instant, tool-free swapping of magnetic clamps and tube racks.
</li>
<li>
Magnetic Flask Clamps: Single-size and combo magnetic clamps designed for quick attachment to hold 25ml up to 500ml Erlenmeyer flasks.
</li>
<li>
Magnetic Tube Racks: Specially designed magnetic racks to accommodate culture tubes, microplates, and large conical bottles in various orientations.
</li>
</ul>
&nbsp;
<h2>How to choose orbital shaker accessories</h2>
&nbsp;
Select Erlenmeyer flask clamps by flask volume
Flask clamps are designed for specific flask sizes, including 125 mL, 250 mL, 500 mL, 1 L, and larger culture flasks. Each clamp is engineered to fit the dimensions of a particular flask size, ensuring secure retention during shaking.
Confirm compatibility with your shaker platform
Accessory mounting systems vary between manufacturers and shaker models. Before ordering, verify that the clamp, holder, or rack is listed as compatible with the specific orbital shaker platform being used.
Choose a microplate holder suitable for your application
Microplate holders secure standard microplates during incubation and mixing procedures. Different holder designs support different operating speeds and plate formats, making compatibility and intended use important selection criteria.
Use a non-slip platform mat for flexible vessel placement
Non-slip mats provide a simple solution for holding bottles, flasks, trays, and other containers at low to moderate shaking speeds. They are useful when vessel configurations change frequently and dedicated clamps are not required.
Verify tube rack capacity and platform fit
Tube rack inserts are available for common laboratory tube formats, including microcentrifuge tubes and conical centrifuge tubes. Confirm that the rack dimensions fit within the shaker platform and that the rack accommodates the desired number of samples per run.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Orbital shaker accessories are designed to keep vessels securely positioned while exposed to continuous circular motion. Flask clamps, plate holders, and tube racks should be selected according to vessel dimensions, operating speed, and platform compatibility. Stainless steel spring-clip clamps remain one of the most widely used accessory formats for laboratory culture applications due to their durability and secure retention.
<a href="https://mbpinc.net/contact-us">Conatct helpful team at MBP</a> for a quick customised quote.

MAGic Clamp™ COMBO, for 125ml, 250ml, 500ml flasks

The H1000-MR-CMB by Benchmark Scientific is the MAGic Clamp™ COMBO — a single magnetic snap-on clamp unit covering 125 ml, 250 ml, and 500 ml Erlenmeyer flasks plus one microplate position (max 4 plates). Compatible with the Incu-Shaker, Orbi-Shaker, and Orbi-Shaker CO2 (BT4001). Installs and swaps without tools using the patented MAGic Clamp magnetic mounting system.

Manufacture

MAGic Clamp™ Tube Rack, 12 x 1.5/2.0ml tubes, horizontal

H1000-MR-T15H MAGic Clamp Tube Rack by Benchmark Scientific holds 12 × 1.5/2.0 ml microcentrifuge tubes in horizontal orientation with magnetic attachment — no screws or pins required. Autoclavable; fits up to 6 racks on compatible Benchmark Orbi-Shaker and Incu-Shaker platforms. Order at mbpinc.net or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.