Brands

<h2 dir="ltr">What Are Luciferase Reporter Lentiviral Vectors?</h2>
 Luciferase reporters have been foundational to gene expression research since their first use as reporter genes in 1985. The bioluminescent signal produced when luciferase oxidises its substrate provides a sensitive, quantitative, non-radioactive readout measurable over a wide dynamic range with standard plate reader luminometers. Lentiviral delivery produces stable, integrated reporter cell lines that maintain consistent signal over weeks to months. Accurate reporter assays depend on appropriate detection systems. Select a luciferase construct that fits your research needs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Luciferase Reporters: Reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background controls with <a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">luciferase reporter systems</a>.</li>
<li>Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li>Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li>Promoter &amp; UTR Reporter Systems: Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li>Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose a Luciferase Reporter</h2>
 Reporter Role Firefly luciferase is typically driven by the promoter or response element under investigation as the experimental reporter; Renilla luciferase &mdash; driven by a constitutive promoter (SV40 or CMV) &mdash; provides within-sample normalisation correcting for cell number, transfection efficiency, and lysis variability. Substrate and Emission Firefly luciferase uses D-luciferin and emits at ~550&ndash;570 nm (green/yellow); Renilla uses coelenterazine and emits at ~480 nm (blue-green) &mdash; both can be measured sequentially from the same lysate in a dual-luciferase assay. Stable vs. Transient Use Lentiviral delivery produces stable reporter cell lines in 1&ndash;2 weeks via antibiotic selection &mdash; essential for high-throughput compound screening, long-term pathway activity monitoring, and in vivo bioluminescence imaging. Pathway-Specific Reporters Promoter or transcriptional response element (TRE) inserts &mdash; such as Wnt/TCF-LEF, NF-&kappa;B, NFAT, or VEGF promoter &mdash; provide a quantitative readout of pathway activity when the cognate transcription factor is activated. In Vivo Imaging Needs CMV-GFP-T2A-Luciferase dual-reporter lentivectors co-express GFP and luciferase for combined fluorescence and bioluminescence tracking of implanted cells in live animals. 
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<h2 dir="ltr">Specifications Context</h2>
 Cells are lysed and mixed with the appropriate substrate in a plate reader or tube luminometer; light emission is measured in relative light units (RLU), proportional to luciferase activity and reflecting promoter activity or transgene expression level. A promoter-less dual luciferase vector establishes the minimum signal level for the assay system, identifying the true zero-activity baseline. Stable lentiviral luciferase reporter cell lines are essential tools for tracking xenograft tumour growth, metastasis, and therapy response in preclinical mouse models. EF1&alpha;-driven constructs maintain high expression for 30+ days without selection, while CMV-driven constructs may undergo progressive silencing in some cell types. Product availability reflects MBP's catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get optimized luciferase reporters for advanced cloning and CRISPR-related workflows.

Luciferase Reporter

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentiviral Vector Amplification Protocol

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Luciferase (Renilla reniformis) Lentivirus (CMV)

LVP010079 by Applied Biological Materials is a pre-packaged Renilla reniformis luciferase (CMV) lentiviral virus. Supplied at 107 IU/mL as 3 × 250 µL. Kanamycin (bacterial) + Puromycin (mammalian) selection. Coelenterazine substrate BLI. Ready to transduce. Plasmid: LV010079. Store at −80°C; shipped on dry ice.

Unit Quantity

Accession Number

Shipping Condition

Luciferase (Renilla reniformis) RFP Lentiviral Vector (Bicistronic)(minCMV)(PGK)

LV010081 by Applied Biological Materials is a 1.0 µg bicistronic lentiviral vector co-expressing Renilla reniformis luciferase (RenLuc, AAO48589) under minCMV and RFP under PGK. Dual Renilla BLI (coelenterazine) and red fluorescence tracking. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Species

Vector Map

Luciferase (Renilla reniformis) RFP Lentivirus (Bicistronic)(minCMV)(PGK)

LVP010081 by Applied Biological Materials is a pre-packaged bicistronic Renilla reniformis luciferase (minCMV) / RFP (PGK) lentiviral virus. Supplied at 107 IU/mL as 3 × 250 µL. Dual Renilla BLI (coelenterazine) + RFP tracking. Ready to transduce. Plasmid: LV010081. Store at −80°C; shipped on dry ice.

<h2>What are Bioluminescence Assays?</h2>
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Bioluminescence assays use luciferase enzymes to convert specific substrates into light, producing a highly sensitive readout for gene expression studies or cell viability measurements. In ATP-based systems, light output correlates with cellular ATP levels and therefore cell viability.
Firefly luciferase uses luciferin along with ATP, magnesium, and oxygen to generate a green/yellow signal with a decay time of roughly 12 minutes. Renilla luciferase uses coelenterazine and oxygen, producing a faster-decaying blue signal and not requiring ATP.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Luciferase Assay Systems: High-performance kits like the Luciferase Assay Kit (G287), designed for rapid, flash-type bioluminescence detection that provides a direct and sensitive measure of gene induction.
</li>
<li dir="ltr" aria-level="1">
Fluorescent Control Proteins: Premium GFP Purified Protein (000033P) available as a reliable internal standard or calibration marker to validate transfection efficiency and protein localization across diverse imaging platforms.
</li>
</ul>
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<h2>How to Choose a Bioluminescence Assay System</h2>
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Decide Between Single and Dual-Reporter Designs
Dual-reporter systems combine a test luciferase with a constitutively expressed control luciferase, allowing normalization for variability in transfection efficiency, cell number, and viability.
Choose NanoLuc for Maximum Sensitivity
NanoLuc is an engineered 19 kDa luciferase that produces approximately 100&ndash;150&times; brighter signal than firefly or Renilla systems and uses furimazine as its substrate.
Match Substrate and Cofactor Requirements
Firefly luciferase requires luciferin, ATP, Mg2+, and oxygen, while Renilla luciferase requires coelenterazine and oxygen only, which is important when ATP levels are part of the experimental variable.
Consider Destabilized Reporters for Faster Response
Destabilized luciferase variants have shorter intracellular half-lives, allowing more rapid detection of transcriptional changes with reduced signal lag.
Use ATP-Based Assays for Viability
ATP-based bioluminescence assays measure cellular viability rather than gene expression, as light output reflects ATP levels in metabolically active cells.
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<h2>Specifications Context</h2>
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The Dual-Luciferase Reporter system combines firefly and Renilla luciferase to allow sequential measurement using a luciferase inhibitor for normalization of experimental variability.
As of 2026, NanoLuc-based systems are increasingly used beyond traditional reporter gene assays, including protein interaction and target engagement studies due to their high brightness and low background.
Enhance your signal detection&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional bioluminescence solutions.

Bioluminescence Assays and Reagents

Luc Gene Test with G287 Report-2

Luciferase Assay Kit

Luciferase Assay Kit (G287) by ABM delivers 500 reactions for firefly luciferase reporter gene quantification in mammalian and bacterial cell cultures, with a two-reagent workflow and luminescence readout within 30 minutes.

Usage

Sterility

Storage Condition

LV010138 Insert Sequence

Luciferase-mCherry Lentiviral Vector (CMV)

LV010138 by Applied Biological Materials is a 1.0 µg dual-reporter lentiviral vector co-expressing mCherry (CMV, constitutive) and Photinus pyralis Luc2 (3×HRE-SV40, hypoxia-inducible). With Kanamycin and Puromycin resistance. For hypoxia BLI and fluorescence tracking studies. Requires lentiviral packaging. Shipped on ice pack; store at −20°C.

Luciferase-mCherry Lentivirus (CMV)

LVP010138 by Applied Biological Materials is a pre-packaged Luciferase-mCherry dual-reporter lentiviral virus. mCherry (CMV, constitutive) + Photinus pyralis Luc2 (3×HRE-SV40, hypoxia-inducible, D-luciferin). Kanamycin + Puromycin selection. 108 IU/mL, 3 × 250 µL. Plasmid: LV010138. Store at −80°C; shipped on dry ice.

Titer

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Lysosome Labeling Lentivirus (LAMP1)(GFP)

LVP718 Lysosome Labeling Lentivirus (LAMP1)(GFP) by Applied Biological Materials delivers LAMP1-GFP for stable green fluorescent lysosomal labeling in transduced mammalian cells. Green-channel counterpart to LVP719 (LAMP1-RFP); spectrally compatible with RFP-based organelle markers for dual-color imaging. Store at &minus;80&deg;C.

Vector

Titer	

Lysosome Labeling Lentivirus (LAMP1)(RFP)

LVP719 Lysosome Labeling Lentivirus (LAMP1)(RFP) by Applied Biological Materials delivers a LAMP1-RFP fusion for stable red fluorescent labeling of the lysosomal compartment in transduced mammalian cells. Spectrally compatible with GFP-based markers for dual-channel imaging; store at &minus;80&deg;C.

<h2>What are bisulfite conversion reagents and buffers?</h2>
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Bisulfite conversion reagents include sodium bisulfite solution, denaturation and conversion buffers, and complete kits that bundle all components needed for the three-step chemical reaction: sulfonation of cytosine to a cytosine-bisulfite derivative, hydrolytic deamination to a uracil-bisulfite derivative, and alkali desulfonation to yield uracil. This process converts unmethylated cytosines to uracil while leaving 5-methylcytosine intact and unconverted, because 5-methylcytosine resists bisulfite-mediated deamination under standard conditions.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">RNA and DNA conversion reagents for enhanced modification of cytosine in methylation workflows</li>
<li dir="ltr" role="presentation">Desulphonation buffers for stabilization of nucleic acids and post-conversion cleanup&nbsp;</li>
<li dir="ltr" role="presentation">Binding buffers used during purification for efficient capture of converted RNA and DNA</li>
<li dir="ltr" role="presentation">Wash buffers for the removal of inhibitors and improved purity of samples</li>
<li dir="ltr" role="presentation">Elution buffers for high-quality recovery of converted nucleic acids</li>
<li dir="ltr" role="presentation">DNA denaturing and digestion buffers for controlled workflow and optimized conversion efficiency</li>
<li dir="ltr" role="presentation">Dilution and reaction buffers for maintaining stable assay conditions</li>
<li dir="ltr" role="presentation">Solubilization and dissolution buffers for sample processing and preparation&nbsp;</li>
<li dir="ltr" role="presentation">Lightning conversion reagents for efficient and rapid methylation analysis workflows</li>
</ul>
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<h2>How to use bisulfite conversion reagents</h2>
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Ensure complete DNA denaturation before conversion to avoid incomplete conversion
Bisulfite only acts on cytosines in single-stranded DNA, so complete denaturation of the input DNA is the most critical first step, since failure to fully denature can leave double-stranded regions in which cytosines are shielded from the bisulfite reagent and remain unconverted.
Understand that incomplete conversion produces false-positive methylation results
If the conversion is incomplete, unconverted unmethylated cytosines will read as cytosine after sequencing, which will be incorrectly interpreted as methylated cytosine, artificially inflating the apparent methylation level of the sample in a false-positive direction.
Optimize conversion conditions to balance completeness against DNA degradation
The harsh conditions needed for complete bisulfite conversion, including high bisulfite concentration, elevated temperature, and long incubation, also fragment and degrade DNA, and optimizing conditions that achieve high conversion while minimizing degradation is a key technical challenge in bisulfite workflows.
Include an unmethylated conversion control to estimate residual incomplete conversion
Including an unmethylated control DNA, such as lambda phage DNA, in the same conversion reaction provides a readout of how completely unmethylated cytosines were converted, helping quantify residual incomplete conversion rates and account for them in downstream analysis.
Be aware of specific challenges for FFPE and degraded DNA samples
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before bisulfite treatment, and the additional fragmentation during conversion can result in poor library yields; specific modified protocols and carrier reagents can help reduce further degradation during the conversion step.
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<h2>Specifications context</h2>
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Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its high resolution and accuracy at single-base level, but it also introduces specific DNA quality challenges for downstream library preparation and sequencing, particularly for samples already of poor quality like FFPE tissue. As of 2026, enzymatic conversion is increasingly recommended as the preferred alternative to bisulfite conversion specifically because it achieves comparable discrimination between methylated and unmethylated cytosines while minimizing the DNA damage and library complexity loss that harsh bisulfite chemistry introduces.
This category sits within DNA/RNA Methylation under Epigenetics, and for a synthetic, fully methylated positive control to run through the conversion workflow, see dNTPs (Deoxynucleotide Triphosphates). See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and request a quote today to get high-quality bisulfite conversion reagents for your lab.

Bisulfite Conversion Reagents & Buffers

D5001-3-MSDS

M-Binding Buffer

The D5001-3 by Zymo Research is M-Binding Buffer (20 ml), the Zymo-Spin column binding reagent for purification of bisulfite-converted DNA in EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits. Sold as a standalone replacement buffer for labs running high-volume bisulfite conversion workflows. Made in the USA.

Manufacturer

M-Binding Buffer (20 ml)

M-Binding Buffer (80 ml)

M-Binding Buffer (250 ml)

M-Binding Buffer (100 ml)

D5005-3-MSDS

M-Binding Buffer-Gold

D5005-3 by Zymo Research is the M-Binding Buffer-Gold (30 ml), used in the Zymo-Spin IC column binding step following bisulfite conversion in the EZ DNA Methylation-Gold Kit (D5005/D5006). Conditions converted single-stranded DNA for silica membrane capture in the Gold Kit's 3-hour workflow (&gt;99% conversion, &gt;75% recovery). Not interchangeable with the M-Binding Buffer from EZ DNA Methylation Kit (D5001). Room temperature storage. Made in USA by Zymo Research.

M-Binding Buffer-Gold (30 ml)

M-Binding Buffer-Gold (125 ml)

M-Desulphonation Buffer

D5001-5 by Zymo Research is M-Desulphonation Buffer (10 ml), the in-column desulphonation reagent for the EZ DNA Methylation Kit, EZ DNA Methylation-Gold Kit, and EZ DNA Methylation-Direct Kit. It is ordered as a standalone replacement when this component is depleted before other kit contents. Also available in 40 ml (D5002-5) and 80 ml (D5040-5) formats.

M-Desulphonation Buffer (10 ml)

M-Desulphonation Buffer (40 ml)

M-Desulphonation Buffer (80 ml)

MSDS

M-Digestion Buffer

D5020-9 by Zymo Research — M-Digestion Buffer (2X); 4 mL; proteinase K digestion buffer for EZ DNA Methylation-Direct workflows; 50 pg–2 µg DNA input; compatible with blood, tissue, FFPE, LCM; 50°C 20 min (standard) or 4 hr (FFPE).