Brands

<h2 data-section-id="1yjaxog" data-start="0" data-end="23">What Are IHC Slides?</h2>
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IHC slides, also known as charged slides or adhesion slides, are microscope slides designed specifically for immunohistochemistry (IHC), histology, cytology, and pathology applications. These slides feature a permanently charged surface that helps tissue sections and cellular samples remain attached during staining procedures, heat-induced epitope retrieval (HIER), antibody incubations, and repeated wash steps.
Globe Scientific Diamond&reg; White Glass charged slides are manufactured from optical-quality white glass and come pre-cleaned and ready to use. They are available with either 90&deg; ground edges or 45&deg; beveled safety corners and are offered in multiple frosted-end color options to simplify specimen identification and laboratory workflow management.
Charged IHC slides are essential for FFPE tissue sections, frozen sections, and cytospin preparations because they help prevent tissue loss during staining and antigen retrieval procedures.
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<h2 dir="ltr">What you will find:</h2>
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Human breast cancer control slides
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FFPE kidney cancer tissue sections
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Human brain tissue microarray slides
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Paraffin-embedded colon cancer sections
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Intestine cancer diagnostic slides
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Positive charged IHC control slides
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Formalin-fixed stomach cancer samples
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Microtome-sectioned pathology slides
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<h2 data-section-id="1p4j59x" data-start="990" data-end="1017">How to Choose IHC Slides</h2>
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Hydrophobic vs Hydrophilic Surface
Most charged IHC slides use a hydrophobic positively charged surface, making them suitable for routine FFPE tissue staining, frozen sections, and cytology applications. These slides provide reliable tissue adhesion during manual immunohistochemistry workflows.
Hydrophilic charged slides are commonly used with automated staining systems where uniform reagent distribution across the slide surface is important. These specialty slides are often selected for automated IHC platforms and high-throughput laboratory workflows.
Adhesion Strength
Standard charged slides provide strong tissue adhesion suitable for most routine immunohistochemistry applications, including common antigen retrieval protocols and standard staining procedures.
For challenging specimens such as fragile frozen sections, decalcified tissues, or protocols involving extended high-temperature antigen retrieval, laboratories may choose specialty slides designed to provide enhanced adhesion performance.
Edge Style
Slides with 90&deg; ground edges provide a smooth finish that works well with automated staining and coverslipping systems.
Slides with 45&deg; beveled safety corners help reduce the risk of cuts during handling and are often preferred in laboratories that process large numbers of slides daily.
Frosted Ends and Color Coding
Frosted labeling areas allow specimen information to be written directly on the slide. Color-coded frosted ends can help distinguish patient samples, staining batches, and laboratory workflows. Multiple color options are available to support organization in busy pathology and histology laboratories.
Packaging Options
IHC slides are available in various box and case quantities to accommodate laboratories of different sizes. High-volume pathology laboratories often purchase slides in case quantities to ensure a consistent supply for routine diagnostic and research work.
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<h2 data-section-id="1jl751w" data-start="3652" data-end="3677">Specifications Context</h2>
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Globe Scientific Diamond White Glass charged microscope slides are manufactured from optical-quality white glass and feature a permanent positive surface charge. Standard dimensions are approximately 25 &times; 75 mm with a thickness of about 1 mm. Slides are supplied pre-cleaned and ready for laboratory use.
Available options include 90&deg; ground edges or 45&deg; beveled safety corners, along with frosted-end labeling areas in white, blue, red, green, yellow, orange, aqua, pink, and lavender.
Packaging formats include boxes containing 72 or 144 slides, with larger case quantities available for high-volume laboratories. Globe Scientific products are manufactured under ISO 9001:2015 quality standards.
Ready to streamline your pathology validation? Request a quote from the <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">helpful team at MBP today</a>.

IHC Slides

Human Meningioma

Human Meningioma FFPE Tissue Section HS0036 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

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<h2>What are methylation standards?</h2>
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Methylation standards are defined reference DNA materials with a known and confirmed methylation status, ranging from a fully methylated DNA (where every cytosine at CpG or other sites carries a methyl group) to a fully unmethylated DNA (where no cytosines are methylated) and intermediate or mixed standards containing a defined ratio of the two. The most widely used unmethylated control in bisulfite sequencing is lambda phage DNA, which is naturally unmethylated and can be spiked into a conversion reaction to estimate conversion efficiency.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" role="presentation">Methylated and non-methylated DNA control sets for comparative epigenetic analysis and assay validation</li>
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<li dir="ltr" role="presentation">Genomic DNA reference materials: for microbial and human methylation studies</li>
<li dir="ltr" role="presentation">Matched DNA sets: used in experimental models for controlled comparison of methylation status&nbsp;</li>
<li dir="ltr" role="presentation">Bisulfite-converted DNA standards: for downstream sequencing-based methylation and PCR analysis</li>
<li dir="ltr" role="presentation">Methylation spike-in controls: for sequencing normalization and high-throughput workflows</li>
<li dir="ltr" role="presentation">Mouse and human DNA methylation reference sets: for specific species epigenetic studies</li>
<li dir="ltr" role="presentation">Methylated DNA IP control kits for methylation assays involving immunoprecipitation</li>
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<h2>How to use methylation standards</h2>
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Spike an unmethylated control into every bisulfite or enzymatic conversion reaction
Running an unmethylated DNA like lambda phage DNA alongside your samples in the same conversion reaction estimates the conversion efficiency of that specific run, since every remaining cytosine signal after conversion should be treated as a false positive, and this rate can be quantified and accounted for in downstream analysis.
Run a fully methylated positive control to confirm methylated cytosines are preserved
A fully methylated standard, such as DNA synthesized with 5-methyl-dCTP or commercially prepared methylated genomic DNA, confirms your conversion workflow correctly preserves methylated cytosines intact rather than inadvertently converting them, which would produce false-negative methylation calls.
Use a mixed or intermediate standard to calibrate quantitative methylation assays
A standard containing a defined ratio of methylated to unmethylated DNA, or a set of standards spanning a range of methylation percentages, provides a calibration curve for quantitative methylation assays like 5-methylcytosine ELISA, validating that the assay reports the expected methylation level across its full dynamic range.
Confirm standard stability before long-term storage or lot-to-lot use
Methylation control DNA has documented stability windows, and confirming that your standard's methylation status is stable under storage conditions, and consistent across lots, is necessary for reliable longitudinal monitoring of conversion efficiency.
Use standards to monitor workflow consistency over time
Running a conversion control in every batch allows longitudinal tracking of conversion efficiency across instrument runs, reagent lots, and protocol variations, helping catch workflow drift before it affects real sample results.
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<h2>Specifications context</h2>
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Lambda phage DNA is the most commonly used unmethylated bisulfite conversion control because it is naturally unmethylated, commercially available, and well characterized, and it can be aligned to the known lambda phage reference genome after sequencing to calculate residual unconverted cytosine rates, providing a direct, interpretable measure of conversion efficiency. As of 2026, fully methylated and fully unmethylated standard sets are also used alongside enzymatic methyl-seq conversions to confirm that EM-seq's enzymatic mechanism correctly protects methylated cytosines and converts unmethylated ones, independent of the conversion chemistry used.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, Non-Bisulfite Kits &amp; Reagents, and ELISA Kits &amp; Reagents. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of Molecular Biology Products Inc. (MBP)</a> to get high-quality methylation standards for your lab today.

Methylation Standards

D5013-Data Sheet

D5013 - MSDS

D5013-Protocol

Human Methylated & Non-Methylated (WGA) DNA Set

D5013 by Zymo Research is a human DNA control set comprising whole-genome amplified (WGA) methylated (&gt;95% CpG methylated) and non-methylated DNA from the HCT116 DKO cell line, plus RASSF1 primers, for use as positive and negative controls in bisulfite sequencing, MSP, and methylation array workflows.

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Human Methylated & Non-Methylated (WGA) DNA Set (DNA w/ Primers)

Human WGA Non-Methylated DNA (5 µg/20 µl)

Human WGA Methylated DNA (5 µg/20 µl)

D5014-Data  Sheet

D5014 - MSDS

D5014-Protocol

Human Methylated & Non-methylated DNA Set

The D5014 by Zymo Research is a Human Methylated &amp; Non-methylated DNA Set containing two HCT116 DKO-derived genomic DNA standards at 250 ng/µl (5 µg each): D5014-1 (&lt;5% global methylation, DNMT1/DNMT3b knockout) and D5014-2 (enzymatically CpG-methylated), plus a DAPK1 primer set for within-run bisulfite conversion and MSP assay validation. Store ≤−20°C up to 24 months.

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Human Methylated & Non-methylated DNA Set (DNA w/ Primers)

Human HCT116 DKO Non-Methylated DNA (5 µg/20 µl)

Human HCT116 DKO Methylated DNA (5 µg/20 µl)

Human Ovarian Cancer

Human Ovarian Cancer FFPE Tissue Section HS0046 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Ovarian High Grade Serous Cancer

Human Ovarian High Grade Serous Cancer FFPE Tissue Section HS0047 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Primary Cells

Human Primary Follicle Outer Root Sheath Cells

T4021-2 by ABM — per ABM spec sheet: Human Primary Dendritic Cells (CD123+/BDCA-2+/BDCA-4+); 5×10⁵ cells/1.0 ml, BSL-II. Seeding 40,000 cells/cm²; PriGrow TM4021 + G299/G422. Note: MBP listing name states ORS cells — verify product identity via CoA. MTA required.

Human Primary Friedriech's Ataxia (FA) Fibroblast Cell Line 281 (Copy)

T5446-1 Human Primary Friedreich's Ataxia Fibroblast Cell Line 281 by ABM — FA patient (male, age 19, onset age 11; GAA alleles 630/806), 5×10⁵ cells/1.0 ml, BSL-II. Seeding 10,000 cells/cm²; PriGrow III + 15% FBS + NEAA required. Dual cryopreservation. MTA required.

Human Rectal Adenocarcinoma

Human Rectal Adenocarcinoma FFPE Tissue Section HS0025 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Rectum

Human Rectum FFPE Tissue Section HS0028 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
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Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
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<h2 data-path-to-node="5">What you will find:</h2>
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Tissue-specific primary cell culture medium kits
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Specialized growth media for endothelial and epithelial cells
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Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
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Differentiation media for progenitor and stem cells&nbsp;
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Serum-free bioprocessing and cryopreservation reagents
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Targeted fibroblast, smooth muscle, and myocyte growth kits
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<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
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Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
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Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Human Smooth Muscle Cell Growth Medium Kit

Human Smooth Muscle Cell Growth Medium Kit (TM108) by Applied Biological Materials is an antibiotic-free kit (TM108 basal medium 500 ml + TM108-S supplement mix) formulated for optimal human smooth muscle cell growth under 5% CO₂. Complete medium expires 30 days post-supplementation. Store basal at 4–8°C; supplement at –20°C.

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Disclaimer

Human Spleen

Human Spleen FFPE Tissue Section HS0051 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Stomach

Human Stomach FFPE Tissue Section HS0029 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.