Brands

Cuvettes

<h2 dir="ltr">What are Cuvettes?</h2>
 Cuvettes are small, precision-dimensioned transparent vessels used to hold liquid samples in the light path of a spectrophotometer, fluorometer, or colorimeter for measurement of absorbance, transmittance, fluorescence intensity, or fluorescence polarization. The standard cuvette has a 10 mm optical path length (the distance light travels through the sample), a square or rectangular cross-section, and an internal volume of 1&ndash;4 mL. Cuvettes are manufactured in four main materials&mdash;polystyrene (PS) or polymethylmethacrylate (PMMA) plastic, optical glass, UV-grade quartz (fused silica), and IR quartz&mdash;each covering a different wavelength range. Choose plastic cuvettes for routine visible-light absorbance (380&ndash;780 nm) where disposability prevents cross-contamination; choose optical glass for reusable visible-range work (340&ndash;2,500 nm); choose UV quartz when measurements extend into the UV range below 340 nm (190&ndash;2,500 nm).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1cg0wqn" data-start="1810" data-end="1862">Spectrophotometer cuvettes for absorbance analysis</li>
<li data-section-id="vbobpt" data-start="1863" data-end="1890">Polystyrene (PS) cuvettes</li>
<li data-section-id="14z5a6i" data-start="1891" data-end="1906">PMMA cuvettes</li>
<li data-section-id="1h4vr7o" data-start="1907" data-end="1966">Analyzer-specific cuvettes for clinical chemistry systems</li>
<li data-section-id="b3k6fl" data-start="1967" data-end="1998">Standard path length cuvettes</li>
<li data-section-id="td8a19" data-start="1999" data-end="2043">Accessories for spectrophotometer cuvettes</li>
</ul>
<h2 dir="ltr"> How to Choose Cuvettes</h2>
 Material and Wavelength Range Plastic (PS/PMMA) cuvettes transmit visible light from 380&ndash;780 nm and are single-use, eliminating cleaning and cross-contamination risk. They are unsuitable for UV measurements, organic solvents, or strong acids. Optical glass cuvettes transmit 340&ndash;2,500 nm, covering the full visible and near-infrared range; Different manufacturers recommend different procedures. UV quartz cuvettes are traditionally used for DNA/RNA quantification at OD260/OD280 because they transmit UV wavelengths below 340 nm. IR quartz extends to 3,500 nm for near-IR applications. Optical Path Length The standard 10 mm path length follows the Beer-Lambert law at normal sample concentrations. Semi-micro (5 mm path) and micro-volume cuvettes (1 mm path, or internal volumes down to 10&ndash;70 &micro;L) are used for precious or limited-volume samples. The path length directly multiplies absorbance: a 1 mm cuvette gives 1/10 the absorbance of a 10 mm cuvette for the same solution &mdash; account for this in Beer-Lambert calculations. Volume: Macro vs. Semi-Micro vs. Micro Macro cuvettes (1&ndash;4 mL) are the standard for routine spectrophotometry. Semi-micro cuvettes (0.5&ndash;1.5 mL) reduce sample consumption. Micro cuvettes (10&ndash;350 &micro;L) are used when sample volume is limited; sub-micro cuvettes (10 &micro;L or less) suit precious clinical or research samples. Confirm that your spectrophotometer's beam height and aperture are compatible with the cuvette format before purchasing micro formats. Two-Window vs. Four-Window Standard cuvettes have two optically clear windows (the light-entry and exit faces) and two frosted or ribbed side walls for gripping. Four-window (all-clear) cuvettes are required for fluorescence measurements at 90&deg; to the excitation beam &mdash; the two additional clear windows allow emitted fluorescence to exit perpendicular to the excitation path. Confirm whether your instrument requires a standard or fluorescence-grade cuvette. Reusable vs. Disposable Quartz and glass cuvettes are reusable; their higher up-front cost&nbsp;is justified for low-throughput precision work or when solvents or UV wavelengths are required. Plastic cuvettes cost fractions of a cent per use and eliminate washing time in high-throughput assay workflows. Select disposable plastic for any routine colorimetric assay (Bradford, BCA, ELISA plate reader backup, OD600 bacterial growth) where wavelengths stay in the visible range. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
The 10 mm path length standard cuvette has internal dimensions of 10 &times; 10 mm cross-section and an internal height of approximately 45 mm. Outer dimensions are 12.5 &times; 12.5 mm, fitting all standard spectrophotometer cell holders. Window parallelism tolerance is &plusmn;0.01 mm for precision quartz cuvettes; standard optical glass cuvettes have &plusmn;0.1 mm tolerance. Light transmission through UV quartz: &gt;90% at 220 nm and &gt;85% at 200 nm. For nucleic acid quantification, a UV quartz cuvette with a minimum path length of 10 mm is required for OD 260/280 measurements; the ratio of OD260/OD280 should be 1.8&ndash;2.0 for pure DNA and 1.9&ndash;2.1 for pure RNA as a purity indicator. As of 2026, plastic disposable cuvettes remain the most cost-efficient choice for labs running &gt;50 absorbance readings per day in the visible range.
&nbsp;
Need the right fit? MBP can guide you to cuvettes that match your workflow perfectly. For institutional pricing and PO-based bulk ordering, <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">contact MBP</a>&nbsp;directly.

5133-Analyzer Consumables Catalog

HITACHI: Cuvette, for use with the Hitachi 902 analyzer, 6/Set, 4 Sets/Unit (Note: New packaging) ** SPECIAL ORDER ITEM **, CS/24

5133 by Globe Scientific is an 8-place PMMA cuvette segment for the Hitachi® 902 clinical chemistry analyzer. Supplied 6 cuvettes/box, 4 boxes/case (24 cuvettes/case, 192 reaction positions). OEM-compatible Globe Scientific replacement. Special Order Item — confirm lead time with MBP before ordering. Not compatible with Hitachi 911/912 (use SKU 5131 for those).

Manufacture

<h2>What are plasmid maxiprep kits?</h2>
&nbsp;
A plasmid maxiprep kit scales SDS/alkaline lysis with silica-membrane spin columns, anion-exchange resin with gravity flow, or fully automated cartridge-based processing to 100-500 ml of overnight E. coli culture, recovering up to 400 ug per spin-column prep or 1-1.4 mg with high-yield anion-exchange chemistry, completable in 35-75 minutes depending on format. Related entities include EndoFree-type endotoxin removal for transfection, automated processors that eliminate manual centrifugation, and downstream applications including large-scale transfection, in vitro transcription/translation, and multiple parallel experiments. Choose Maxiprep when your application needs the largest routine plasmid yields (hundreds of micrograms to over 1 mg) from a single overnight culture.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Ultrafast 25-minute maxiprep kits drastically reduce processing times using specialized column technology&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Syringe-filter plasmid maxi units rapidly clear dense bacterial lysates using vacuum-free clarification systems&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid maxiprep systems for delivering transfection-grade DNA in sensitive mammalian cell lines</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-performance silica-column vectors optimized for maximum concentration and recovery of low and high-copy plasmids</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Gravity-flow anion-exchange assemblies without manual centrifugation steps, ensuring chromatography-level purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid maxiprep kit</h2>
&nbsp;
When maxiprep is the right scale
A maxiprep should be used when purifying plasmid from an overnight bacterial culture with a volume between 100-500 ml, when downstream needs exceed midiprep's 0.5-2 mg range - for example, large-scale transfection across many plates, in vitro transcription/translation requiring substantial template DNA, or banking DNA for multiple future experiments from one culture. If midiprep yields already meet your needs, the larger culture volume and processing time of maxiprep may not be justified.
Spin-column vs. anion-exchange vs. automated chemistry
Spin-column maxipreps (e.g., 60-minute procedures) recover up to roughly 400 ug per prep, while anion-exchange or high-yield chemistries can reach 1-1.4 mg from comparable culture volumes; automated cartridge-based processors can deliver endotoxin-free DNA in under 75 minutes without manual centrifugation, pouring in culture, and pressing start. Match chemistry to both your yield target and available equipment - automated processors reduce hands-on time but represent a larger equipment investment.
Endotoxin levels at maxi scale
Endotoxin concentrations tend to be higher in larger-scale preparations - standard maxipreps have been reported at 11-16 EU/ug, while EndoFree-type kits target below 0.1 EU/ug through additional washing, and "substantially endotoxin-free" is sometimes defined as under 100 EU/ug for less stringent applications. For transfection of highly sensitive cells, vaccination research, or gene therapy applications, confirm the maxiprep kit's specific endotoxin target rather than assuming "maxiprep" implies low endotoxin by default.
Lysate clearing and centrifuge requirements
Maxi-scale lysate clearing typically requires high-speed centrifugation of the full culture volume (100-500 ml) followed by a clearing column or filtration step; gravity-flow anion-exchange systems use folded filters to avoid this centrifugation, while automated processors handle clearing internally. Confirm your centrifuge has rotors compatible with 100-500 ml culture volumes before committing to a centrifugation-based maxiprep format.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for maxiprep selection are expected yield (400 ug for spin-column vs. 1-1.4 mg for high-yield/anion-exchange chemistry), endotoxin target relative to your transfection application's sensitivity, and lysate-clearing method relative to your centrifuge's capacity for 100-500 ml culture volumes. A maxiprep should specifically be used for culture volumes in the 100-500 ml range; smaller volumes are better served by <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;or&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>. Labs running large-scale transfection or in vitro transcription/translation programs typically standardize on either high-yield anion-exchange maxipreps or automated processors to balance yield, endotoxin level, and hands-on time. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;covers the full mini-to-maxi range, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a>&nbsp;link to chromosomal DNA workflows. Use MBP's&nbsp;<a href="https://mbpinc.net/quickorder">Quick Order tool</a>&nbsp;for case-quantity pricing on maxiprep kits or&nbsp;<a href="https://mbpinc.net/contact-us">contact the specialist team</a>&nbsp;for guidance on endotoxin levels and automation options for your transfection program.
<section class="mbp-contact-cta"></section>
&nbsp;
Scale-up workflow efficiency with MBP&rsquo;s dependable macro-extraction plasmid technologies. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> today to find the perfect match for your lab.

Maxiprep Kits

Product Literature

HP Plasmid Maxi Kit

HP Plasmid Maxi Kit (PD1713-00) by MBP purifies plasmid DNA from 150–250 mL bacterial cultures in less than 60 minutes. Listed for transfection, RFLP, DNA amplification, and automated sequencing. Available in 1 (PD1713-00), 10 (PD1713-01), and 25 (PD1713-02) prep sizes.

Manufacturer

HP Plasmid Maxi Kit , 1 Preps

HP Plasmid Maxi Kit , 10 Preps

HP Plasmid Maxi Kit , 25 Preps

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

HP Plasmid Midi Kit

HP Plasmid Midi Kit (PD1712-00) by MBP uses a proprietary ezBind™ matrix for exclusive and efficient DNA binding. Proteins and impurities removed by wash buffer; eluted with sterile water or Elution Buffer. Available in 2 (PD1712-00), 10 (PD1712-01), and 25 (PD1712-02) prep sizes.

HP Plasmid Midi Kit , 2 Preps

HP Plasmid Midi Kit , 10 Preps

HP Plasmid Midi Kit , 25 Preps

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

HP Plasmid Mini Kit

HP Plasmid Mini Kit (PD1711-00) by MBP uses a proprietary DNA binding system for high efficient reversible binding to a mini column. Proteins and impurities removed by wash buffer; eluted with sterile water or elution buffer. Available in 4 (PD1711-00), 50 (PD1711-01), and 250 (PD1711-02) prep sizes.

HP Plasmid Mini Kit , 4 Preps

HP Plasmid Mini Kit , 50 Preps

HP Plasmid Mini Kit , 250 Preps

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

MSDS

HRP Developer

D5425-4-15 by Zymo Research is HRP Developer (15 ml), a colorimetric substrate solution for use with the Quest 5-hmC DNA ELISA Kit and Quest 5-mC DNA ELISA Kit. Sold individually for component replenishment or as part of a complete kit. Enables quantitative measurement of genomic 5-hmC and 5-mC levels. Also available in 30 ml (D5425-4-30). MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Shipping Condition

Storage Condition

HRP Developer (15 ml)

HRP Developer (30 ml)

<h2>What Are Laboratory Recirculating Chillers?</h2>
&nbsp;
Laboratory recirculating chillers are compressor-based cooling instruments that circulate chilled fluid through a closed loop to remove heat from connected equipment or maintain below-ambient temperatures in a reactor, condenser, or instrument cooling line. Unlike tap-water cooling, recirculating chillers reuse coolant, eliminating water waste and ensuring stable setpoint temperatures independent of building water supply variations. Applications include rotary evaporator condenser cooling, jacketed reactor temperature control, protection of vacuum pump oil from vapor contamination, cooling of laser cavities, NMR superconducting magnets, and mass spectrometers. Modern air-cooled chillers achieve a coefficient of performance (COP) of approximately 3:1, meaning 1 kW of electrical input removes 3 kW of heat load. Choose a benchtop recirculating chiller for small-scale solvent recovery and instrument cooling; choose a floor-standing high-capacity model for jacketed reactors and multi-instrument cooling networks.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Ai Recirculating Chillers offer a user-friendly and compact solution for everyday cooling, featuring powerful centrifugal pumps that keep your routine benchtop tasks moving smoothly.
</li>
<li dir="ltr" aria-level="1">
Julabo Dynamic Temperature Control units bring incredible speed to your workflow, allowing you to jump between extreme heat and cold with the precision your specialized research demands.
</li>
<li dir="ltr" aria-level="1">
HUBER Unistat and OLED Chillers make monitoring a breeze with crystal-clear displays and smart interfaces, putting total control of your chemical reactors right at your fingertips.
</li>
<li dir="ltr" aria-level="1">
PolyScience Immersion Coolers give you the flexibility to drop temperatures quickly in any bath or tank using high-tech probes and large, easy-to-read digital displays.
</li>
<li dir="ltr" aria-level="1">
Huber Air-Cooled Immersion Units handle the toughest ultra-low cooling jobs with ease, providing heavy-duty performance without the hassle of extra plumbing or water lines.
</li>
</ul>
&nbsp;
<h2>How to Choose a Lab Recirculating Chiller</h2>
&nbsp;
Cooling capacity at target temperature
Manufacturers publish cooling capacity in watts or kW at specific setpoint temperatures. A chiller with 500 W capacity at -20 degrees C may deliver only 200 W at -40 degrees C. Match cooling capacity to the actual heat load of the connected equipment at the required operating temperature, not the ambient-temperature rating.
Temperature range
Benchtop recirculating chillers reach -10 degrees C to -30 degrees C for most solvent recovery and condenser cooling applications. Deep-cooling models reach -40 degrees C to -80 degrees C for cryogenic trapping, lyophilizer condenser cooling, and temperature-sensitive biological workflows. Mechanical cold traps used with vacuum lines typically require -40 degrees C or colder.
Air-cooled vs. water-cooled
Air-cooled chillers dissipate heat via a fan into the room air and require no plumbing. They are the standard for bench-scale labs, but raise the room temperature and reduce efficiency when the ambient temperature exceeds 25 degrees C. Water-cooled chillers are more efficient at high heat loads and in warm environments, but require facility supply and drain connections.
Reservoir volume and flow rate
Reservoir volume (4 L to 60 L) determines thermal buffering capacity; larger volumes smooth out temperature fluctuations during high-load cycles. Flow rates of 10 L/min to 42 L/min are typical. External applications with long tubing runs or large jacketed vessels require higher flow rates to minimize temperature rise along the circuit.
Coolant compatibility
Most lab chillers use deionized water or water-ethylene glycol mixtures as coolant. For applications requiring temperatures below -20 degrees C, a 30-50% ethylene glycol mixture is standard to prevent freezing in the circuit. Confirm the chiller's internal materials (stainless steel, polypropylene, or PTFE wetted parts) are compatible with the intended coolant.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Confirm coolant type compatibility, pump flow rate at target temperature (not ambient), and electrical requirements: 120V/60Hz for North America or 208-240V for high-capacity models. Modern environmentally responsible chillers use natural hydrocarbon (HC) refrigerants with low global warming potential (GWP). Closed-loop systems with optical fluid level sensors reduce maintenance downtime compared to mechanical float switches. Chillers maintaining temperature within plus or minus 0.1 degrees C or better are appropriate for precision analytical instrument cooling.
&nbsp;
Ready to beat the heat in your lab? Take a look through our chiller collection and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a friendly quote today.

Chillers

HUBER Chiller with OLED Display

HUBER -20C to 100C Minichiller 600-H Chiller/Heater OLED Display

HUBER -20C Minichiller 600 Compact Chiller with OLED Display

Product News: KISS® Cooling and heating circulators

HUBER Minichiller 600 and Minichiller 600-H — compact OL-controller chillers with OLED display. Minichiller600: cooling to −20°C. Minichiller600H: −20°C to +100°C heating and cooling. For routine lab temperature control.

HUBER CoolSmart Chiller

HUBER CS-Model Standard Package

HUBER CoolSmart CS series compact recirculating chillers to −20°C. Four variants: CS25 (Huber-CS25), CS50 (Huber-CS50), CS100 (Huber-CS100), CS200 (Huber-CS200). Reduced water consumption, low noise, for condenser and process cooling.

HUBER CoolSmart CS25 -20C Chiller

HUBER CoolSmart CS50 -20C Chiller

HUBER New CoolSmart CS -20°C Chillers

HUBER CoolSmart CS75 -20C Chiller

<h2>What Are Laboratory Heaters?</h2>
&nbsp;
Laboratory heaters are temperature-control instruments that raise the temperature of a fluid (deionized water, buffer, glycol, or silicone oil) and maintain it at a stable setpoint for connected equipment or for samples immersed in the bath. Open bath heaters expose the fluid surface and allow direct sample contact; closed-system heaters circulate heated fluid through external tubing to jacketed vessels, columns, or instruments without evaporation loss. Immersion circulators clip onto an existing open bath and add a heating element and pump without requiring a dedicated bath unit. Applications span reagent and media warming, enzyme activity assays, blood banking thaw protocols, hybridization reactions, and food stability testing. Choose an open bath heater for direct sample immersion at up to 100 degrees C; choose a closed-system heater for external circulation, jacketed vessels, or temperatures above 100 degrees C.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Julabo Heating Circulators use stainless steel bath tanks and powerful pumps to provide uniform warmth for high-temperature research.
</li>
<li dir="ltr" aria-level="1">
Refrigerated-Heated Circulators offer versatile temperature ranges, allowing you to switch between deep cooling and intense heating in one system.
</li>
<li dir="ltr" aria-level="1">
Ai Compact Heated Recirculators deliver reliable heating up to 100&deg;C in a space-saving design perfect for small-scale benchtop projects.
</li>
<li dir="ltr" aria-level="1">
Induction Heaters with Timers provide rapid, localized heating for specialized material science using advanced frequency technology.
</li>
<li dir="ltr" aria-level="1">
HUBER Heating Units and Fluids combine user-friendly digital interfaces with high-quality transfer media for smooth, long-term thermal management.
</li>
<li dir="ltr" aria-level="1">
PolyScience Heated Circulators feature robust safety controls and digital displays for precise management of high-temperature laboratory runs.
</li>
</ul>
&nbsp;
<h2>How to Choose a Lab Heater</h2>
&nbsp;
Heating method: open bath vs. closed system vs. immersion
Open bath heaters (sometimes called circulating water baths) are the most common format for biological and enzymatic work up to 100 degrees C. They allow direct tube or flask immersion and are easy to clean. Closed-system heaters use a smaller reservoir, a stronger pump, and a powerful heating element to push heated fluid externally, preferred for jacketed vessels, columns, or high-temperature applications. Immersion circulators convert an existing open bath into a constant-temperature bath at a lower cost than a full benchtop circulator.
Temperature range
Most open bath heaters cover ambient temperatures to 99 degrees C, which covers cell culture, hybridization, and most enzymatic workflows. Closed-system heaters reach 150 degrees C to 200 degrees C for materials testing, food stability, and polymer research. Certain high-temperature models reach 250 degrees C and above, but require compatible heat-transfer fluids such as silicone oil rather than water.
Bath volume and heating capacity
Match bath volume to your sample load. Small-footprint open baths start at 2 L to 4 L; larger models run to 28 L or more for high-throughput workflows. Heating capacity in kW determines warm-up time: 1 kW to 2 kW is typical for standard benchtop units, while closed-system heaters with external circulation often specify 3 kW or higher for rapid heat-up.
Temperature uniformity and stability
Circulating heaters outperform non-circulating models on temperature uniformity across the bath. Digital PID controllers maintain stability to plus or minus 0.1 degrees C at most biological setpoints. Open baths without circulation are acceptable for batch incubations but not for assays requiring tight temporal control.
Control and connectivity
Entry-level units offer a simple digital setpoint; advanced models include programmable temperature profiles (ramp and soak), over-temperature alarms, lockout features, RS-232/RS-485 connectivity, and compliance with IEC 61010-1 and DIN 12876.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Temperature stability specifications are measured at a steady-state setpoint with the lid closed; actual stability in use depends on room temperature fluctuations, lid openings, and sample volume. Rated working temperature in DIN 12876-1 Class I and Class II units must be at least 25 degrees C below the bath fluid flash point, relevant if ethanol or glycol solutions are used as bath fluid. For biological applications using water as the bath fluid, this constraint is not relevant. Units listing IEC 61010-2-010 certification meet safety requirements for laboratory heating equipment.&nbsp;
&nbsp;
Looking to turn up the precision in your lab? Explore our heaters and <a href="https://mbpinc.net/contact-us">reach out to the friendly MBP team</a> for a custom quote today.

Heaters

HUBER Heating Circulator

HUBER KISS 205B 200°C 5L Capacity Heating Circulator

HUBER KISS 205B 200°C 5L Capacity Heating Circulator Standard Package

HUBER CC-308B 300°C 8.5L Heating Circulator with Pilot ONE

HUBER CC-308B 300°C 8.5L Heating Circulator with Pilot ONE Standard Package

HUBER KISS K6 -25C to 200°C 4.5L Refrigerated Heated Circulator

HUBER KISS K6 -25C to 200°C 4.5L Refrigerated Heated Circulator Standard Package

Three Huber bath circulators in one listing: KISS-205B (45–200°C, 5L, 1.5–2.1kW), KISS-K6 (−25 to 200°C, 4.5L, refrigerated/heated, R-290), and CC-308B (28–300°C, 8.5L, 3kW, Pilot ONE touchscreen, ±0.02°C stability). DIN 12876 Class III/FL safety. Available at MBP INC.

HUBER KISS 205B 200Â°C 5L Capacity Heating Circulator

HUBER KISS K6 -25C to 200Â°C 4.5L Refrigerated Heated Circulator

HUBER CC-308B 300Â°C 8.5L Heating Circulator with Pilot ONE

HUBER Stepless controlled VPC Bypass M30x1,5

HUBER Stepless controlled VPC bypass valve, M30×1.5 thread, for Huber Unistat temperature control systems. Not pre-mounted — retrofit installation. Temperature range: −90°C to +200°C.

HUBER M90 -90°C to +200°C Heat Transfer Fluid 10L

HUBER M90 -90°C to +200°C Heat Transfer Fluid 10L Standard Package

HUBER M20 -20°C to +235°C Heat Transfer Fluid 10L

HUBER M20 -20°C to +235°C Heat Transfer Fluid 10L Standard Package

HUBER M60 -60°C to +200°C Heat Transfer Fluid 10L

HUBER M60 -60°C to +200°C Heat Transfer Fluid 10L Standard Package

HUBER Transfer Fluid 10L

Huber thermal transfer fluids in 10 L cans: M90 (−90°C to +200°C, M90-10L-6479), M20 (−20°C to +235°C, M20-10L-6162), and M60 (−60°C to +200°C, M60-10L-6166). For Huber Unistats and jacketed reactor temperature control circuits.

HUBER DW-Therm M90 -90C to +200C Thermal Transfer Fluid 10L

HUBER M20 -20C to +235C Heat Transfer Fluid 10L

HUBER M60 -60C to +200C Heat Transfer Fluid 10L

HUBER Unistat with Pilot ONE

HUBER Unistat 405 -45°C to 250°C with Pilot ONE

HUBER Unistat Tango -45°C to 250°C with Pilot ONE

HUBER Unistat 815 -85°C to 250°C with Pilot ONE

HUBER Unistat 915w -90°C to 250°C with Pilot ONE

HUBER Unistat T305 300°C with Pilot ONE – 208V

HUBER Unistat 825 -85°C to 250°C with Pilot ONE

HUBER Unistat 950w -90°C to 200°C with Pilot ONE

HUBER Unistat 905 -90°C to 250°C with Pilot ONE

Pilot ONE multi-touch controller von Huber

HUBER Unistat series single-fluid dynamic temperature control systems with Pilot ONE 5.7-inch touchscreen. Nine variants from −45°C/+250°C (Unistat 405) to −90°C/+250°C (Unistat 915w) and +300°C (T305, 208V). For jacketed reactor control, synthesis, and winterization.

HUBER Unistat 405 -45Â°C to 250Â°C with Pilot ONE

HUBER Unistat Tango -45Â°C to 250Â°C with Pilot ONE

HUBER Unistat 815 -85Â°C to 250Â°C with Pilot ONE

HUBER Unistat 915w -90Â°C to 250Â°C with Pilot ONE

HUBER Unistat T305 300Â°C with Pilot ONE - 208V

HUBER Unistat 825 -85Â°C to 250Â°C with Pilot ONE

HUBER Unistat 950w -90Â°C to 200Â°C with Pilot ONE

HUBER Unistat 905 -90Â°C to 250Â°C with Pilot ONE

HUBER Unistat 510 -50Â°C to 250Â°C with Pilot ONE

HUBER with Pilot ONE

HUBER Ministat 230 -40°C to 200°C with Pilot ONE

HUBER Ministat 230 -40°C to 200°C with Pilot ONE Standard Package

HUBER CC-508 -55°C to +300°C 5L with Pilot ONE

HUBER CC-508 -55°C to +300°C 5L with Pilot ONE Standard Package

HUBER CC-902 -90°C to +200°C 5L with Pilot ONE

HUBER CC-902 -90°C to +200°C 5L with Pilot ONE Standard Package

Huber refrigerated/heating circulators with Pilot ONE 5.7&#34; colour touchscreen, available in −40°C to +200°C (3.2 L), −55°C to +200°C (5 L), and −90°C to +200°C (5 L) configurations. All achieve ±0.02°C stability with M16×1 pump connections and RS232/Ethernet/USB connectivity.