Brands

Filtration Centrifuge Accessories

<h2>What Are Filtration Centrifuge Accessories?</h2>
&nbsp;
Filtration centrifuge accessories are the consumable and add-on components that extend or maintain the functionality of centrifugal filter devices. These include replacement filter inserts with specific molecular weight cut-off (MWCO) membranes, centrifuge tube adapters, 96-well filter plates for high-throughput processing, and collection tubes for retentate and filtrate recovery. Since membrane inserts are designed for specific device formats and brands, selecting compatible accessories is essential for reliable performance, sample integrity, and efficient laboratory workflows.
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<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Perforated centrifugal baskets for separation processes based on filtration</li>
<li>Non-perforated centrifugal baskets for regulated containment of samples</li>
<li>Filter materials available in various sizes, including 7 &micro;m, 20 &micro;m, 30 &micro;m, and 50 &micro;m, to meet specific filtration requirements</li>
</ul>
&nbsp;
<h2>How to Choose Filtration Centrifuge Accessories</h2>
&nbsp;
Replacement Filter Inserts by MWCO
Select the MWCO based on the target molecule and application requirements. Lower MWCO values are suitable for retaining small proteins and peptides, while higher MWCO options support larger biomolecules, complexes, and viral particles. Always use replacement inserts designed for the original device format and manufacturer.
Centrifuge Tube Adapters
Adapters help position filtration devices correctly within the centrifuge rotor. Proper seating prevents uneven loading, improves filtration efficiency, and supports consistent sample recovery throughout the centrifugation process.
96-Well Filter Plates
Laboratories processing multiple samples simultaneously benefit from 96-well filtration systems. These formats support applications such as protein removal, nucleic acid cleanup, and pharmaceutical screening while maintaining compatibility with standard microplate centrifuge rotors.
Collection Tube Receivers
Collection tubes capture filtrate and concentrated samples during operation. For highly sensitive applications, dedicated single-use collection tubes help minimize cross-contamination and maintain sample purity.
Pre-Wetting Protocols
Many membrane systems perform best when pre-wetted with an appropriate buffer before sample loading. This process improves flow consistency, reduces resistance, and helps prepare the membrane for efficient filtration.
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<h2>Specifications Context</h2>
&nbsp;
Most centrifugal filtration devices operate within recommended centrifugal force ranges of 3,000&ndash;5,000 &times;g, although specific limits vary by product design and membrane type. Following manufacturer guidelines helps prevent membrane damage and ensures optimal sample recovery.
Modern filtration accessories incorporate advanced membrane technologies, improved collection systems, and high-throughput formats that support biotechnology, pharmaceutical, and life science applications. These developments continue to improve efficiency, reproducibility, and laboratory productivity.
The search for perfect accessories compatible with your filtration centrifuge is over. <a href="https://mbpinc.net/contact-us">Contact now</a> with the expert team at Molecular Biology Products Inc. to find a perfect match.

Filter material PP DLW, 20um pore size, Pack of 10

The Z600-S3-F20u by Hermle is a deep-layer-wound (DLW) polypropylene filter with a 20 µm nominal pore size, sold in a pack of 10, for use with the Hermle SIEVA-3 Filtration Centrifuge. DLW construction provides graduated particle retention from outer to core layers, extending service life versus flat-membrane alternatives at comparable pore ratings. Suitable for clarification of cell culture media, biological buffers, and reagents.

Manufacture

Filter material PP DLW, 30um pore size, Pack of 10

Z600-S3-F30u by Hermle is a polypropylene DLW filter material with 30 µm pore size for the Hermle SIEVA-3 (Z600-S3) Filtration Centrifuge, sold in packs of 10. Retains particles ≥ 30 µm from biological and process samples. Order at mbpinc.net or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Filter material PP DLW, 50um pore size, Pack of 10

Z600-S3-F50u by Hermle is polypropylene (PP) DLW filter material with a 50 µm pore size for the Hermle SIEVA-3 filtration centrifuge (Z600 series), supplied as 10 filters per pack. The 50 µm pore is the coarsest in the SIEVA-3 filter range, suited for pre-filtration, coarse debris removal, and clarification of biological samples before finer downstream filtration steps

Filter material PP DLW, 7um pore size, Pack of 10

The Z600-S3-F7u by Hermle is a 10-pack of polypropylene dry-laid web (PP DLW) filter material with a 7 µm pore size for the Hermle Z600-S3 SIEVA-3 filtration centrifuge, supporting cake filtration at up to 10,000 rpm / 7,825 xg.

Filtered Pipette Tips

<h2 dir="ltr"> What Are Filtered Pipette Tips?</h2>
 Filtered pipette tips contain a hydrophobic aerosol-barrier plug &mdash; polyethylene (PE) frit or cellulose &mdash; embedded inside the tip body, positioned to intercept aerosol and liquid that would otherwise travel up through the tip collar into the pipette barrel during dispensing or when handling volatile or splashing samples. The filter protects the pipette barrel from internal contamination (which requires costly professional decontamination) and protects samples from cross-contamination by previously deposited nucleic acids, proteins, or biohazardous material in the barrel. Filtered tips are the standard consumables for PCR and qPCR setup, RNA extraction, cDNA synthesis, working with radioactive or biohazardous agents, and any workflow where a single contaminated barrel could compromise a run or an experiment. Non-filtered tips remain appropriate for routine buffer, media, and reagent dispensing where cross-contamination through the barrel is not a concern. Use filtered tips for all nucleic acid work, PCR, and biohazardous samples; use standard tips for routine buffer and reagent dispensing.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li><a href="https://mbpinc.net/consumables/liquid-handling-products/pipette-tips/universal-fit-pipette-tips" title="Universal Fit Filter Tips">Universal Fit Filter Tips:</a> &ndash; Designed to fit a wide range of standard pipettes, with options including wide-bore, low-retention, sterile, RNase/DNase-free, and aerosol barrier formats to support diverse liquid handling applications while maintaining sample integrity.</li>
<li><a href="https://mbpinc.net/consumables/liquid-handling-products/filtered-pipette-tips/lts-compatible-filter-tips" title="LTS Compatible Filter Tips:">LTS Compatible Filter Tips:</a>&nbsp;Optimized for high-performance pipetting systems, featuring extended-length and rack-packed configurations for deep-vessel access, with bulk-pack options available to support reliable, high-throughput laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Filtered Pipette Tips</h2>
 Filter Material: Polyethylene Frit vs Cellulose Hydrophobic PE frits are the most common and most studied aerosol-barrier material; they block liquid ingress reliably without absorbing sample material or releasing fibres. Cellulose filters are used in some barrier tips (including the original Thermo Barrier tips); they are effective aerosol barriers but can absorb a small volume of aqueous sample, relevant at &le;10 &micro;L, where the absorbed volume is proportionally significant.
Universal Fit vs LTS-Compatible Filtered Tips Universal filtered tips use standard collar geometry (3.8 mm for &le;10 &micro;L, 6.1 mm for &le;200 &micro;L) and fit Eppendorf, Rainin Classic (non-LTS), and Thermo pipettes. LTS-compatible filtered tips use Rainin's cylindrical LTS mounting geometry and are required for Rainin LTS pipettes.&nbsp;
Low-Retention Filtered Tips Low-retention filtered tips combine an aerosol barrier with a hydrophobic inner tip wall, reducing liquid adhesion during dispensing. They are recommended for small-volume (&le;10 &micro;L) nucleic acid work, RNA, protein, and enzyme dispensing, where both contamination protection and volume accuracy are critical.
Volume Range Filtered tips are available across 10 &micro;L (P2&ndash;P10), 20 &micro;L (P20), 100 &micro;L (P100), 200 &micro;L (P200), 300 &micro;L, 1,000 &micro;L (P1000), and 1,250 &micro;L extended formats. Extended-length filtered tips reach deep into 15 mL and 50 mL conical tubes for nucleic acid elution and sample transfer steps.
Packaging Filtered tips are typically sterile-racked (96/rack, 10 racks/box); reload/refill formats are available for high-volume filter tip users.
<h2 dir="ltr"> Specifications Context</h2>
 Globe Scientific filter tips are certified free of human DNA, RNase, DNase, pyrogens, and PCR inhibitors; sterile, in flip-top or hinged racks. Sorenson Bioscience universal filter tips span 10&ndash;1,250 &micro;L in standard rack and EcoRack reload formats, pre-sterilised, DNase/RNase-free. Invitrogen Barrier Tips (Thermo Fisher) are the original reference for nucleic acid work with cellulose filter elements; compatible with Eppendorf pipettes. Globe Scientific, Nest Scientific, and Sorenson Bioscience filtered tips are stocked by MBP Inc.&nbsp; 
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Don&rsquo;t let cross-contamination stall your progress. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Reach out to the MBP team</a> today for a quote or to find the perfect fit for your specific pipette brand.

DataSheet

Filter tips, Racked, Sterile

FT-10-R-S by LABSELECT — 10 µL sterile racked filter tips, virgin PP with hydrophobic PE filter, 96 tips/rack, 10 racks/unit (960 tips/unit). Sterile, DNase-free, RNase-free, nonpyrogenic, noncytotoxic. Universal pipette fit.

Packaging

Volume

Color

Manufacturer

10 ul Filtered Tip, Racked, PreSterilized.96 x 10 Tips/Pk, 5PK/CS

10 ul Filtered Tip, Racked, PreSterilized. Extended length tip.96 x 10 Tips/Pk, 5PK/CS

20 ul Filtered Tip, Racked, PreSterilized.96 x 10 Tips/Pk, 5PK/CS

100 ul Filtered Tip, Racked, PreSterilized.96 x 10 Tips/Pk, 5PK/CS

200 ul Filtered Tip, Racked, PreSterilized.96 x 10 Tips/Pk, 5PK/CS

1000 ul Filtered Tip, Racked, PreSterilized. Extended length tip.96 x 10 Tips/Pk, 5PK/CS

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile tubing and connector sets
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

BioFactory Cap with Silicon Tube (80cm 1/4" ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with Silicon Tube (80cm 3/8" ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ42mm), without BioFactory, Sterile

BioFactory Cap with PTFE Vent Filter (0.22 μm Φ50mm), without BioFactory

Filter/inlet & outlet tubing for BioFactory

C71254 by NEST Scientific provides filter, inlet, and outlet tubing accessories for the NEST BioFactory&#8482; multi-layer cell culture system, enabling closed aseptic liquid transfer during vaccine, mAb, and large-scale adherent cell culture workflows. The vent cap incorporates a 0.22&#160;µm PTFE hydrophobic filter; the system is E-beam sterilized to SAL&#160;=&#160;10&#8315;&#8310; with no endotoxin and no animal-origin components.

BioFactory Cap with Silicon Tube (80cm 1/4 " ID3/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with Silicon Tube (80cm 3/8 " ID5/8" OD), Male CPC Connector with Female Sealing Cap, without BioFactory, Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ42mm), without BioFactory , Sterile, 1/pk, 4/cs

BioFactory Cap with PTFE Vent Filter(0.22 μm Φ50mm), without BioFactory , Sterile, 1/pk, 4/cs

Transfer Pipettes

<h2>What are disposable transfer pipettes?</h2>
&nbsp;
Disposable transfer pipettes are single-use plastic liquid-handling tools made from low-density polyethylene (LDPE). They are designed as a one-piece bulb-and-stem system used for transferring, dispensing, and handling small volumes of liquids in laboratory workflows.
They are commonly used as a safer and more convenient alternative to glass Pasteur pipettes. Being plastic and disposable, they eliminate the risk of breakage, reduce cross-contamination, and remove the need for separate rubber bulbs. The flexible bulb allows controlled aspiration and dispensing of reagents, samples, and solutions.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Graduated transfer pipettes &ndash; for approximate volume measurement and controlled dispensing</li>
<li>Non-graduated transfer pipettes &ndash; for simple liquid transfer without measurement markings</li>
<li>Fine-tip transfer pipettes &ndash; for small droplets and precise spot dispensing</li>
<li>Sterile transfer pipettes &ndash; for microbiology and contamination-sensitive workflows</li>
<li>Non-sterile transfer pipettes &ndash; for routine laboratory use</li>
<li>Disposable Pasteur pipettes &ndash; for general single-use liquid handling</li>
</ul>
&nbsp;
<h2>How to choose transfer pipettes</h2>
&nbsp;
Tip style Fine-tip pipettes are used for precise work such as microplate spotting and small-volume handling. Standard tips are suitable for general transfers, while wide-bore designs are better for viscous liquids or blood samples. Longer stems help access deep containers.
Capacity and volume handling Select pipettes based on typical working volumes. Smaller pipettes are suitable for low-volume transfers, while larger ones are used for routine sample handling. The effective draw volume depends on bulb capacity and usage technique.
Graduated vs non-graduated Graduated pipettes allow approximate volume estimation and are useful in clinical and diagnostic workflows. Non-graduated pipettes are intended for general dispensing where precise measurement is not required.
Sterility requirements Sterile pipettes are used in microbiology, cell culture, and clinical applications where contamination control is essential. Non-sterile versions are suitable for general laboratory and teaching use.
Length and accessibility Long pipettes are preferred for deep vessels such as flasks and bottles, while shorter designs are used for compact tubes and small workspaces.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Disposable LDPE transfer pipettes are widely used laboratory consumables for general-purpose liquid handling in research, clinical, and educational environments. They are available in sterile and non-sterile formats with multiple tip designs and capacities to support different workflows.
These pipettes remain a standard alternative to glass Pasteur pipettes due to their safety, low cost, and reduced contamination risk in routine laboratory applications.
For bulk orders or lab-specific requirements, <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for assistance and pricing.

134010-Group-Transfer Pipet Brochure

134010-Group-TDS-134050

134010-Group-TDS-134070

134010-Group-TDS - 134020

134010-Group-TDS - 134010

Fine Tip Transfer Pipets Specifications

Fine Tip Transfer Pipets

134010 by Globe Scientific are LDPE fine tip transfer pipets — fine tip for precise narrow-stream delivery, sterile and non-sterile options, bench-top dispenser box with hinged front panel. Single-use for gel loading, supernatant transfer, and targeted liquid dispensing.

Transfer Pipet, 1.3mL, Fine Tip, 51mm, 500/Dispenser Box, 8 Boxes/Unit

Transfer Pipet, 1.3mL, Fine Tip, 51mm, 500/Dispenser Box

Transfer Pipet, 1.5mL, Fine Tip, 104mm, 400/Dispenser Box, 8 Boxes/Unit

Transfer Pipet, 1.5mL, Fine Tip, 104mm, 400/Dispenser Box

Transfer Pipet, 1.5mL, Fine Tip, 104mm, STERILE, Individually Wrapped, 4000/Cs

Transfer Pipet, 1.5mL, Fine Tip, 104mm, STERILE, 20/Pack, 25 Packs/Unit

Transfer Pipet, 5.8mL, Fine Tip, 147mm, 500/Dispenser Box, 10 Boxes/Unit

Transfer Pipet, 5.8mL, Fine Tip, 147mm, 500/Dispenser Box

Transfer Pipet, 5.8mL, Fine Tip, 147mm, STERILE, Paper Peel Wrap, Individually Wrapped, 100/Bag, 4 Bags/Unit

Transfer Pipet, 5.8mL, Fine Tip, 147mm, STERILE, 20/Pack, 20 Packs/Unit

Transfer Pipet, 5.8mL, Fine Tip, 157mm, 500/Dispenser Box, 10 Boxes/Unit

Transfer Pipet, 5.8mL, Fine Tip, 157mm, 500/Dispenser Box

Transfer Pipet, 5.8mL, Fine Tip, 157mm, STERILE, Individually Wrapped, 100/Bag, 4 Bags/Unit

Transfer Pipet, 5.8mL, Fine Tip, 157mm, STERILE, 20/Pack, 20 Packs/Unit

Transfer Pipet, 5mL, Fine Tip, 153mm, 250/Dispenser Box, 8 Boxes/Unit

Transfer Pipet, 5mL, Fine Tip, 153mm, 250/Dispenser Box

Transfer Pipet, 5mL, Fine Tip, 153mm, STERILE, Individually Wrapped, 400/Case

Transfer Pipet, 5mL, Fine Tip, 153mm, STERILE, 20/Bag, 20 Bags/Unit

Transfer Pipet, 8.7mL, Fine Tip, 147mm, 400/Dispenser Box, 10 Boxes/Unit

Transfer Pipet, 8.7mL, Fine Tip, 147mm, 400/Dispenser Box

Transfer Pipet, 8.7mL, Fine Tip, 147mm, STERILE, Individually Wrapped, 100/Bag, 4 Bags/Unit

Transfer Pipet, 8.7mL, Fine Tip, 147mm, STERILE, 20/Pack, 20 Packs/Unit

Centrifuge Tubes

<h2>What Are Centrifuge Tubes?</h2>
 Centrifuge tubes are cylindrical laboratory containers with caps that are used to separate, pellet, and store biological or chemical samples through centrifugal force. They are commonly made from polypropylene (PP) or polystyrene (PS) and are available in sizes ranging from small 0.5 mL microtubes to larger 50 mL conical tubes. These tubes are widely used for cell pelleting, protein precipitation, DNA and RNA extraction, tissue homogenate clarification, and long-term sample storage. Depending on the material, they can withstand temperatures from &minus;80 &deg;C to +121 &deg;C.
In short, the best centrifuge tube depends on your centrifuge rotor, required g-force, sample volume, and sterility needs. 
<h2>What You Will Find: </h2>
<ul>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes" title="All Centrifuge Tubes">All Centrifuge Tubes</a>: Comprehensive selection of 15 mL and 50 mL conical tubes with clear graduations for daily lab workflows.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/oak-ridge-style-centrifuge-tubes" title="Oak Ridge Style Centrifuge Tubes">Oak Ridge Style Centrifuge Tubes</a>: High-speed, chemical-resistant tubes designed to withstand extreme RCF without deformation.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/centrifuge-bottles" title="Centrifuge Bottles">Centrifuge Bottles</a>: Heavy-duty, larger-capacity vessels engineered for bulk sample processing and high-velocity runs.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes" title="Large Volume Centrifuge Tubes">Large Volume Centrifuge Tubes</a>: Industrial-strength 250 mL and 500 mL tubes optimized for pilot-scale harvesting and bioprocessing.</li>
</ul>
&nbsp;
<h2>How to Choose Centrifuge Tubes</h2>
 Volume and Rotor Compatibility
Select a tube size that matches both your sample volume and centrifuge rotor. Microtubes ranging from 0.5&ndash;2 mL are commonly used in microcentrifuges and can typically handle speeds up to 20,000 &times; g. Midi tubes in the 5&ndash;10 mL range are suitable for intermediate sample volumes, while 15 mL and 50 mL conical tubes are commonly used in clinical, research, and preparative centrifuges.
Material: Polypropylene vs. Polystyrene
Polypropylene (PP) tubes offer excellent chemical resistance and can tolerate temperatures from &minus;80 &deg;C to +121 &deg;C. They are suitable for autoclaving and exposure to many laboratory solvents. Polystyrene (PS) tubes provide superior optical clarity, making it easier to visually inspect samples and liquid levels, but they have lower resistance to solvents and extreme temperatures.
Sterility and Contamination Control
Sterile centrifuge tubes are typically gamma- or E-beam-irradiated and certified free of RNase, DNase, and pyrogens. These tubes are recommended for RNA extraction, cell culture, and clinical applications where contamination must be minimized. Non-sterile tubes may still be RNase- and DNase-free and are often suitable for routine DNA work and general sample storage.
Cap Design
Attached screw caps help prevent cap mix-ups and sample contamination, especially in high-throughput laboratories. Separate caps can be quicker to open and may be preferred in workflows that require frequent access to samples. If tubes will be used with automated systems, cap dimensions should be verified for compatibility with liquid handling equipment.
Packaging Options
Racked tubes provide convenient organization and allow direct pipetting while the tubes remain in the rack. Bulk-bagged tubes offer a more economical option for laboratories that process large volumes of samples and already have tube storage systems in place.
<h2> Comparing Common Centrifuge Tube Types</h2>
 Microtubes (0.5&ndash;2 mL) are ideal for PCR preparation, microcentrifuge applications, and storage of small sample volumes. They can generally withstand centrifugal forces up to 20,000 &times; g.
Midi tubes (5&ndash;10 mL) are designed for intermediate sample volumes such as culture supernatants and can typically tolerate forces up to 16,000 &times; g.
Sterile 15 mL conical tubes are frequently used for cell pelleting, RNA extraction, and clinical sample processing, with typical ratings between 12,000 and 15,000 &times; g.
Sterile 50 mL conical tubes are commonly selected for larger-volume applications including tissue culture work, density gradient preparation, and large-scale sample processing.
Polystyrene conical tubes in 15 mL and 50 mL formats are often chosen when visual sample inspection is important and solvent exposure is minimal.
<h2> Specifications and Features</h2>
 Polypropylene centrifuge tubes from Globe Scientific and Nest Scientific are manufactured from USP Class VI virgin resin and do not contain plasticizers or processing additives. Sterile products are typically irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶. Most 15 mL tubes feature graduation marks at 1 mL intervals, while 50 mL tubes are usually graduated every 5 mL. MBP offers both bagged and racked centrifuge tube formats, including 15 mL and 50 mL conical tubes as well as smaller microtube and midi tube options.
MBP supports procurement departments by accepting purchase orders and offering institutional pricing for qualified high-volume customers. <a href="https://mbpinc.net/contact-us">Contact the MBP team today</a> for a customized quote.

Tubes, Caps & Racks Catalog

Tube Caps Specifications

Flange Plug Cap - For Test Tubes

Globe Scientific's polyethylene (PE) flanged plug caps provide exceptional leakresistance in test tubes, centrifuge tubes and round cuvettes. 

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, Natural, 10000/CS

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, Blue, 10000/CS

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, Green, 10000/CS

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, Red, 10000/CS

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, White, 10000/CS

Cap, Flange Plug, 17mm, for 17mm Tubes and 15mL Centrifuge Tubes, Yellow, 10000/CS

Cap, Flange Plug, 12mm, Blue, CS/20000

Cap, Flange Plug, 12mm, Clear, CS/20000

Cap, Flange Plug, 12mm, Green, CS/20000

Cap, Flange Plug, 12mm, Black, CS/20000

Cap, Flange Plug, 12mm, Lavender, CS/20000

Cap, Flange Plug, 12mm, Orange, CS/20000

Cap, Flange Plug, 12mm, Red, CS/20000

Cap, Flange Plug, 12mm, White, CS/20000

Cap, Flange Plug, 12mm, Yellow, CS/20000

Cap, Flange Plug, 13mm, Blue, CS/20000

Cap, Flange Plug, 13mm, Clear, CS/20000

Cap, Flange Plug, 13mm, Green, CS/20000

Cap, Flange Plug, 13mm, Black, CS/20000

Cap, Flange Plug, 13mm, Lavender, CS/20000

Cap, Flange Plug, 13mm, Orange, CS/20000

Cap, Flange Plug, 13mm, Red, CS/20000

Cap, Flange Plug, 13mm, White, CS/20000

Cap, Flange Plug, 13mm, Yellow, CS/20000

Cap, Flange Plug, 16mm, Blue, CS/10000

Cap, Flange Plug, 16mm, Clear, CS/10000

Cap, Flange Plug, 16mm, Green, CS/10000

Cap, Flange Plug, 16mm, Black, CS/10000

Cap, Flange Plug, 16mm, Lavender, CS/10000

Cap, Flange Plug, 16mm, Orange, CS/10000

Cap, Flange Plug, 16mm, Red, CS/10000

Cap, Flange Plug, 16mm, White, CS/10000

Cap, Flange Plug, 16mm, Yellow, CS/10000

15ml & 50ml Centrifuge Tubes

<h2 data-section-id="155qizo" data-start="0" data-end="33">What Are All Centrifuge Tubes?</h2>
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The All Centrifuge Tubes collection includes the complete range of tube sizes commonly used in research, clinical, and laboratory environments. These tubes range from small 0.5 mL and 1.5&ndash;2 mL microtubes used in microcentrifuge applications to 5 mL and 10 mL midi tubes for intermediate sample volumes, as well as 15 mL and 50 mL conical tubes designed for cell culture, density gradient preparation, and larger-volume sample processing. They are available in polypropylene (PP) for enhanced chemical resistance and temperature tolerance or polystyrene (PS) for improved optical clarity.
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What you will find:
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<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microcentrifuge-tubes" title="Microcentrifuge Tubes">Microcentrifuge Tubes</a> &ndash; Precision-engineered for small-volume sample preparation.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/screw-cap-tubes" title="Screw Cap Tubes">Screw Cap Tubes</a> &ndash; Secure, leak-proof closures for long-term storage and transport.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/15ml-and-50ml-centrifuge-tubes" title="15ml &amp; 50ml Centrifuge Tubes">15ml &amp; 50ml Centrifuge Tubes</a> &ndash; Standard conical bottom tubes for large-volume processing.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/midicentrifuge-tubes-5ml-and-10ml" title="Midicentrifuge Tubes (5ml &amp; 10ml)">Midicentrifuge Tubes (5ml &amp; 10ml)</a> &ndash; The perfect bridge for intermediate sample sizes.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microtubes-box" title="Microtubes Box">Microtubes Box</a> &ndash; Durable storage racks for organized sample management and protection.
</li>
</ul>
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<h2 data-section-id="1f93yys" data-start="914" data-end="963">How to Choose From All Centrifuge Tube Formats</h2>
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Volume and Rotor Compatibility
Selecting the correct tube size is essential for safe and efficient centrifugation. Microtubes ranging from 0.5&ndash;2 mL are designed for microcentrifuges, while 5&ndash;10 mL midi tubes fit compatible benchtop centrifuge rotors. Larger 15 mL and 50 mL conical tubes are commonly used in clinical and research centrifuges. Always verify rotor compatibility before introducing a new tube format into your workflow.
Sterility Requirements
For sensitive applications such as RNA extraction, cell culture, and clinical diagnostics, sterile tubes certified free of RNase, DNase, and pyrogens are recommended. Non-sterile tubes can provide a cost-effective solution for routine DNA analysis, protein assays, and general sample storage where sterility is not critical.
Material Selection
Polypropylene tubes offer excellent durability, can be autoclaved at 121 &deg;C, and resist many laboratory chemicals and solvents. Polystyrene tubes provide superior transparency, making it easier to monitor liquid levels and sample contents, although they are less resistant to heat and solvents.
Packaging and Inventory Planning
Microtubes are commonly available in pack sizes ranging from 500 to 1,000 units, while 15 mL and 50 mL conical tubes are often supplied in cases of 500. Laboratories with consistent usage may benefit from establishing recurring purchase orders to ensure uninterrupted supply.
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<h2 data-section-id="urfo3f" data-start="2395" data-end="2433">Compare Centrifuge Tube Size Groups</h2>
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Microtubes (0.5&ndash;2 mL) are primarily used for PCR preparation, microcentrifuge applications, and storage of small sample volumes. These are typically manufactured from polypropylene and are available in pack sizes of 500&ndash;1,000 units.
Midi tubes (5&ndash;10 mL) support intermediate-volume centrifugation and supernatant collection. They are commonly made from polypropylene and supplied in packs ranging from 100&ndash;500 units.
15 mL conical tubes are widely used for cell pelleting, RNA isolation, and clinical sample processing. They are available in both polypropylene and polystyrene formats and are generally packaged in cases of 500.
50 mL conical tubes are designed for larger-volume workflows, including tissue culture, density gradient preparation, and sample processing. Like 15 mL tubes, they are available in polypropylene and polystyrene and are commonly sold in cases of 500.
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<h2 data-section-id="1ezckh3" data-start="3318" data-end="3348">Specifications and Features</h2>
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Globe Scientific and Nest Scientific centrifuge tubes are manufactured from USP Class VI virgin polypropylene or polystyrene resin and are certified free from detectable RNase, DNase, and pyrogens. Sterile products are irradiated using gamma or E-beam methods to achieve a sterility assurance level (SAL) of 10⁻⁶. MBP offers both racked and bagged formats for 15 mL and 50 mL conical tubes, along with dispenser-box options for selected microtube product lines that support efficient use in high-throughput laboratory environments.
For procurement teams, MBP accepts purchase orders and offers institutional pricing for bulk and high-volume purchases. <a href="https://mbpinc.net/contact-ushttps://mbpinc.net/contact-us" title="Contact the MBP team" style="color: #236fa1;">Contact the MBP team</a> today to learn more

All Centrifuge Tubes

112011-Group-Tubes, Caps & Racks Catalog

112011-Group-TDS-112030

Flared-Top Urine Centrifuge Tubes

Globe Scientific 112011 flared-top urine centrifuge tube, PS, 12 mL, 21 mm × 105 mm. Graduated; flared rim for spill-free decanting; rated to 1,500 RCF. Case of 500. No cap included.

Tube, Urine Centrifuge, 12mL, with Flared Top, PS, Graduated, 500/Unit, CS/500

Urine Collection System, 12mL Flared Top Urine Centrifuge Tube and Separate Blue Snap Cap, CS/1000

Tube, Urine Centrifuge, 12mL with Flared Top, PS, Kova Type, Graduated, 1000/Bag, 2 Bags/Unit, CS/2000

Tube, Urine Centrifuge, 12mL, with Flared Top, PS, Graduated, 500/Bag, 3 Bags/Unit, CS/1500

Tube, Urine Centrifuge, 12mL, with Sediment Bulb and Flared Top, PS, Graduated to 10 mL, 500/Bag, 3 Bags/Unit

Tube, Urine Centrifuge, 12mL, with Sediment Bulb and Flared Top, PS, Graduated to 10 mL

Tube, Urine Centrifuge, 12mL, with Flared Top, PS, Graduated, 500/Bag, 2 Bags/Unit, CS/1000

Urine Collection System, 12mL Flared Top Urine Tube and Yellow Snap Cap with Sanitary Grip, 100/Bag, 5 Bags/Unit, CS/500

Cap, Snap, PE, Blue, for Flared Top Urine Tube, 500/Bag, 3 Bags/Unit, CS/1500

Cap, Snap with Sanitary Grip, PE, Yellow, for Flared Top Urine Tube, 500/Bag, 3 Bags/Unit, CS/1500

Lytic Enzymes

<h2>What Are Lytic Enzymes?</h2>
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Lytic enzymes degrade the structural polysaccharide scaffolding of microbial cell walls, enabling cell disruption under mild conditions without mechanical force. Lysozyme (N-acetylmuramide glycanohydrolase) cleaves the beta-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine residues in bacterial peptidoglycan, lysing gram-positive bacteria efficiently and gram-negative bacteria when paired with EDTA to destabilize the outer membrane. Lyticase from Arthrobacter luteus hydrolyzes beta-1,3-glucan in yeast cell walls and is the standard reagent for generating spheroplasts from Saccharomyces cerevisiae and other yeast species. Lysostaphin is a glycyl-glycine endopeptidase that specifically cleaves the polyglycine cross-links in Staphylococcus aureus peptidoglycan. Mutanolysin cleaves beta-1,4 linkages in the alternating N-acetylmuramyl-N-acetylglucosamine backbone of gram-positive bacteria, complementing lysozyme for complete lysis of resistant species. Choose the lytic enzyme based on organism type (bacterial vs. yeast/fungal), Gram stain classification, and intended downstream workflow (DNA extraction, protein isolation, or transformation).
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Zymolyase&trade; Ultra: An elite, high-activity enzyme optimized for rapid fungal cell wall digestion and maximum nucleic acid recovery.
</li>
<li dir="ltr" aria-level="1">
Flash-Extract Lysis Solution: A streamlined reagent for immediate genomic DNA release from diverse samples, ideal for NGS integration.
</li>
<li dir="ltr" aria-level="1">
Zymolyase&trade; w/ Storage Buffer: A stabilized formulation designed to maintain long-term enzymatic potency and consistent performance across all assays.
</li>
</ul>
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<h2>How to Choose Lytic Enzymes</h2>
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Gram-positive vs. gram-negative bacteria
Lysozyme alone lyses most gram-positive bacteria efficiently at concentrations of 0.1-1 mg/mL in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA at 37 degrees C for 30-60 minutes. Gram-negative bacteria require EDTA (typically 10-50 mM) to chelate divalent cations and disrupt the outer membrane before lysozyme can access the peptidoglycan. Species with thick, atypical cell walls, such as Micrococcus, Staphylococcus, and Streptococcus, may respond better to lysostaphin, mutanolysin, or mechanical disruption combined with enzymatic lysis.
Yeast and fungal lysis
Lyticase (minimum 2,000 U/mg protein) is the reagent of choice for yeast spheroplast generation and DNA/RNA extraction from Saccharomyces, Candida, Pichia, Kluyveromyces, and related species. A typical protocol uses 10-100 U of lyticase per OD600 unit of yeast cells in 1 M sorbitol buffer at 30 degrees C for 30-60 minutes. Kitalase (from Rhizoctonia solani) combines beta-1,3-glucanase with protease, pectinase, and amylase activity, providing broader lytic activity for resistant fungal species.
DNA purity requirements
For microbiome research and 16S/18S rDNA amplification, lytic enzymes must be certified free of contaminating DNA. Lyticase lots used in microbiome studies are QC-tested by 35-cycle PCR amplification of 16S and 18S rDNA using universal primer sets; confirm this certification with each lot before use in amplicon sequencing workflows.
Downstream compatibility
Most lytic enzymes are inactivated by subsequent SDS treatment or heating to 95 degrees C for 5 minutes, making them compatible with standard nucleic acid extraction kits. Lyticase requires removal before silica column binding; heat inactivation at 70 degrees C for 10 minutes or proteinase K digestion at 55 degrees C for 30 minutes accomplishes this. Confirm inactivation conditions in the manufacturer's protocol before proceeding.
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<h2>Specifications Context</h2>
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Lysozyme activity is measured in units per milligram of protein, where 1 unit is defined as the decrease in absorbance of 0.001 per minute at 450 nm when Micrococcus lysodeikticus cells are used as substrate at pH 6.24 and 25 degrees C. Lyticase activity is expressed as units per milligram protein; 1 unit releases 1 nmol of glucose from yeast cell walls per minute at 30 degrees C. Lysozyme is stable for 12 months at 4 degrees C as a lyophilized powder and should be dissolved in 10 mM sodium acetate, pH 4.5-5.0, for storage in solution. Lytic enzyme combinations with mechanical disruption (bead beating) are standard for complete lysis of difficult samples, including Mycobacterium, spore-forming bacteria, and thick-walled fungi in clinical and microbiome research workflows. For downstream removal of protein from lysates, see <a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors/proteases">proteases,</a> including Proteinase K, for nucleic acid cleanup.
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Streamline your path to discovery&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today to secure a quote for our advanced lytic reagents.

User Manual

MSDS

Flash-Extract Lysis Solution

AZ-3410 by Azura Genomics: single-tube lysis solution releasing PCR-ready genomic DNA from mouse tails, ear punches, plant leaves, bacteria, and saliva in 8 minutes. Complete workflow under 60 minutes. Available in 100-, 400-, and 2,000-reaction packs. Store at −20°C.

Flash-Extract Lysis Solution 100 reactions

Flash-Extract Lysis Solution 400 reactions

Direct PCR Master Mixes

<h2>What Are Direct PCR Master Mixes?</h2>
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Direct PCR master mixes are specially formulated 2x PCR reagents designed to amplify nucleic acid targets directly from crude biological samples such as blood, buccal swabs, tissue punches, cultured cells, or other minimally processed material without a separate DNA or RNA extraction step. This is achieved through inhibitor-tolerant polymerase formulations, often combined with proprietary buffer additives that counteract the effects of hemoglobin, melanin, polysaccharides, and other common PCR inhibitors present in crude lysates.&nbsp;Choose a direct PCR master mix for high-throughput genotyping or rapid screening applications where eliminating the extraction step provides meaningful time and cost savings, provided the application tolerates the typically modest reduction in amplification efficiency compared to extracted-template PCR.
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<h2>What You Will Find</h2>
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<ul>
<li>Direct PCR &amp; Fast Extraction Kits: Streamlined solutions such as the Flash-Extract&trade; PCR Kit and Accuris Fast Extraction PCR Kit that enable rapid PCR workflows from minimally processed samples, reducing preparation time and simplifying genotyping applications.</li>
<li>Genotyping Kits: Specialized products including the Azura&trade; Mouse Genotyping Kit and Accuris 1 Hour Mammalian Genotyping Kit, designed for reliable genotyping workflows using tissue, tail, ear, or other biological samples.</li>
</ul>
&nbsp;
<h2>How to Choose a Direct PCR Master Mix</h2>
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Sample Type Compatibility
Direct PCR formulations vary in the crude sample types they are validated for. Blood, tissue, cells, and swabs each present different inhibitor profiles, so confirm the master mix is validated for your specific sample type before adopting it for a new workflow.
Inhibitor Tolerance Mechanism
Inhibitor tolerance is typically achieved through a combination of enzyme engineering and buffer additives that bind or neutralize inhibitory compounds. The specific combination affects which inhibitor types, such as heme from blood or melanin from pigmented tissue, the mix is best suited to handle.
Lysis Step Requirements
Some direct PCR master mixes require a brief lysis step, such as a short heat or detergent treatment, before amplification, while others are formulated for direct addition of the crude sample to the PCR reaction. Confirm the workflow complexity matches your throughput needs.
Amplicon Size and Application
Direct PCR is well established for short-to-moderate amplicon genotyping and screening applications. Very long amplicons or applications requiring maximum yield from limited template may still benefit from a brief extraction step even when a direct-compatible mix is available.
Comparison to Extraction-Based PCR
Direct PCR master mixes generally show good performance for routine genotyping but may exhibit somewhat reduced sensitivity or yield compared to PCR from purified template. For applications where extraction-free convenience outweighs a modest performance trade-off, direct PCR offers substantial time savings, particularly at high sample throughput.
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<h2>Specifications Context</h2>
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Direct PCR master mixes enable PCR directly from crude samples through heat-stable polymerases tolerant of inhibitor compounds and lysis buffer components, combined with optimized buffer systems designed to neutralize hemoglobin, melanin, polysaccharides, and other inhibitors commonly found in blood, tissue, and plant material. The time savings from eliminating extraction are most significant in high-throughput genotyping pipelines, where extraction across hundreds of samples can represent a substantial fraction of total workflow time. As with any inhibitor-tolerant formulation, performance can vary by specific sample matrix. Validating a direct PCR master mix against a small panel of representative samples before deploying it across a full study is considered good practice. Direct PCR master mixes are typically stored at -20&deg;C. Lot-specific Certificates of Analysis (CoAs) are available on request.&nbsp;
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Streamline your amplification workflow&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Direct PCR solutions.

Flash-Extract PCR Kit - Product Sheet

Flash-Extract™ PCR Kit

AZ-3310 by Azura Genomics is the Flash-Extract™ PCR Kit: integrated DNA extraction and PCR amplification from mouse tissue, plant, bacteria, and saliva in under 60 minutes. No centrifugation required. Available in 100-, 400-, and 2000-reaction packs.

Flash-Extract PCR Kit 100 reactions

Flash-Extract PCR Kit 400 reactions

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
&nbsp;
<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p1"> What are Non-Treated Cell Culture Plates?</h2>
 Non-treated cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces retain the original hydrophobic, unmodified polystyrene character &mdash; no plasma, corona, or chemical treatment is applied. The hydrophobic surface (contact angle ~75&ndash;90&deg;) prevents cell attachment, making these plates the correct choice for suspension cell lines, hematopoietic cells, hybridoma culture, spheroid and organoid formation, and any experiment where surface adhesion would alter the cell phenotype or experimental readout. Non-treated plates are the correct default for all suspension-growing cell lines and for any 3D culture format &mdash; spheroids, organoids, embryoid bodies &mdash; where surface attachment is undesired.
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<h2 class="p1">What you will find:</h2>
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<ul>
<li>6, 12, 24, 48, and 96 well formats for different workflows</li>
<li>Untreated surfaces with no coating or modification</li>
<li>Clear plates for easy observation and monitoring</li>
<li>Uniform wells for consistent sample handling</li>
<li>Sterile options to maintain clean conditions</li>
<li>Lids designed to reduce contamination and evaporation</li>
</ul>
<h2 class="p1"> How to Choose Non-Treated Cell Culture Plates</h2>
 Suspension Cell Lines Requiring Non-Treated Plates Non-treated flat-bottom plates support maintenance and assays for Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells, THP-1 monocytes, MOLM-13, and Daudi cells. Primary hematopoietic cells &mdash; PBMCs, CD34⁺ progenitors, NK cells, and bone marrow isolates &mdash; require non-treated surfaces to prevent integrin-triggered differentiation signals from TC-treated polystyrene. For hybridoma culture during HAT selection, non-treated 96-well U-bottom plates are the standard format. U-Bottom vs. Flat-Bottom for Non-Treated Plates Non-treated flat-bottom plates are the standard for suspension cell viability assays, cytotoxicity screens, and ELISA from non-adherent lysates, where wells need a uniform optical path and a flat imaging surface. Non-treated U-bottom 96-well plates concentrate non-adherent cells to the well center by gravity, producing cell pellets that are easier to aspirate during wash steps &mdash; essential for immunophenotyping panels with multiple antibody labeling and wash cycles before flow cytometry. Spheroid and 3D Culture in Non-Treated Plates Non-treated round-bottom (U-bottom) 96-well plates are widely used for hanging-drop spheroid formation and gravity-aggregated tumor spheroid culture. The hydrophobic, non-adhesive well surface combined with U-bottom geometry causes cells to aggregate at the well vertex by gravity, forming compact spheroids over 24&ndash;72 hours without scaffolding. Spheroids formed in non-treated U-bottom 96-well plates are directly measurable in the plate using a plate reader or confocal microscope with an appropriate working distance. Assay Compatibility Non-treated plates are fully compatible with cell viability reagents (CellTiter-Glo, CellTiter-Aqueous MTS, PrestoBlue), cytokine ELISA from cell supernatants, luciferase reporter assays from suspension-cell lysates, and flow cytometry sample preparation. For assays requiring a flat optical surface with minimal well-to-well distance variation, flat-bottom non-treated plates are preferred over U-bottom. Sterility and Purity Grade NEST Scientific non-treated plates are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free, non-pyrogenic, and individually wrapped in peelable sterile film. They are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms under ISO 9001:2015 and ISO 13485 quality management. No additional surface chemistry or coating is applied; the non-treated specification is confirmed by water contact angle measurement per NEST lot release protocol. 
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific non-treated plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms; the non-treated well surface has a water contact angle of ~75&ndash;90&deg; (hydrophobic) confirmed per lot release. All plates conform to ANSI/SLAS SBS footprint standards (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers and plate readers. Alphanumeric well coordinates are printed on all plates &ge;12-well format. Package box double functions as a reagent reservoir for multi-channel pipette operations per NEST design. As of 2026, NEST Scientific non-treated plates in all core formats are stocked by MBP. 
&nbsp;
Use non-treated plates when you want full control over how your cells interact with the surface. Keep conditions simple. Keep results predictable. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> now to get a tailored quote.

Non-Treated Cell Culture Plates

Flat Bottom Cell Culture Plates, Sterile, Non-Treated

NEST Scientific 703011 is a sterile 6-well flat-bottom cell culture plate with non-treated hydrophobic polystyrene surface. Features a low-evaporation lid and corner feet for microscopy. Individually wrapped, 1/pk, 50/cs — for suspension culture, spheroid formation, and applications where cell attachment must be prevented.