Brands

High-Fidelity Master Mix

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
</ul>
<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

User Manual

ExtremeTaq HiFi Red Mix - Product Sheet

MSDS

ExtremeTaq™ HiFi Red Mix

AZ-1910 ExtremeTaq™ HiFi Red Mix by Azura Genomics is a 2x hot-start PCR master mix with up to 10x fidelity vs Taq, antibody-mediated hot-start, proofreading 3'→5' exonuclease, direct-load red dye, 10 kb amplicon capacity, available in 200–8000 reaction pack sizes.

Manufacturer

ExtremeTaq HiFi Red Mix 200 Reactions

ExtremeTaq HiFi Red Mix 1000 Reactions

ExtremeTaq HiFi Red Mix 2000 Reactions

Test Tubes

<h2 dir="ltr">What are Test Tubes?</h2>
 Test tubes for laboratory use are open-ended, cylindrical glass or plastic tubes used to hold, mix, incubate, and heat liquid and solid samples across a broad range of applications: bacteriological culture, tissue culture, clinical chemistry, serological assays, immunology, and general sample processing. Choose borosilicate glass for reusable, high-temperature, or chemically demanding applications; choose polystyrene disposable 12&times;75 mm tubes for flow cytometry and colorimetric assays; choose polypropylene capped tubes for microbial culture with anaerobic or gas-generating organisms.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">Glass test tubes in diverse formats for heating and chemical reaction workflows</li>
<li dir="ltr" aria-level="1">
Plastic polypropylene test tubes for routine laboratory use
</li>
<li dir="ltr" aria-level="1">
Graduated tubes for quick and easy volume estimation
</li>
<li dir="ltr" aria-level="1">
Conical and round-bottom tube formats for different applications
</li>
<li dir="ltr" aria-level="1">
Sterile disposable test tubes for clinical and diagnostic work
</li>
<li dir="ltr" aria-level="1">
Heat-resistant tubes for incubation and controlled heating steps
</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose Test Tubes</h2>
 Material: Glass vs. Polypropylene vs. Polystyrene Borosilicate glass 3.3 (ASTM E438 Type I, Class B) test tubes withstand direct flame, autoclave sterilization at 121 &deg;C, and most solvents, acids, and bases. They are reusable and autoclavable, making them cost-effective over high-volume laboratory runs. Polystyrene (PS) disposable tubes offer outstanding optical clarity for flow cytometry (FACS), cell counting, and OD-based colorimetric assays, but cannot be autoclaved and should not be used with aromatic solvents or strong acids. Polypropylene (PP) tubes are chemically resistant, autoclavable, and available with screw caps for anaerobic culture or gas-generating reactions. Rimmed vs. Rimless Rimless test tubes (fire-polished ends) allow easier insertion of rubber or silicone stoppers and are preferred for plugging with cotton wool for microbial culture. Rimmed tubes have a flared lip that facilitates pouring and is compatible with spring-metal tube closures. Confirm your closure type before ordering in bulk. Size and Volume The most common test tube dimensions in clinical and research labs: 12&times;75 mm: ~5 mL capacity; standard for flow cytometry, FACS, serology 13&times;100 mm: ~10 mL; general culture and chemistry 16&times;100 mm: ~14 mL; culture and chemistry 16&times;125 mm: ~17 mL; microbiological culture 18&times;150 mm: ~25 mL; larger-volume digests and culture Wall thickness of 1.2 mm (light wall, borosilicate 3.3) is the standard for general lab use; heavy-wall versions are used for digestions and applications involving vacuum or pressure. Sterile vs. Non-Sterile Sterile borosilicate glass culture tubes (individually wrapped or bulk-sterilized) are required for microbial culture, cell culture, and any application where contamination would invalidate the result. Non-sterile tubes are adequate for chemistry, spectroscopy, and other abiotic applications. Disposable PS tubes from Corning (PYREX&trade;) and Globe Scientific are supplied sterile or non-sterile; confirm the specification on the lot certificate. 
<h2 dir="ltr"> Specifications Context</h2>
 Borosilicate glass 3.3 test tubes have a linear thermal expansion coefficient of 3.3 &times; 10⁻⁶ K⁻&sup1;, providing resistance to thermal shock from room temperature to 300 &deg;C (direct flame) and from 0 to 121 &deg;C (autoclave cycles). Light-wall tubes (1.2 mm uniform wall thickness) provide the standard for general laboratory use; heavy-wall variants (2.0 mm) are used for digestion vessels and pressurized applications. Corning PYREX&trade; disposable round-bottom rimless glass tubes for tissue culture conform to ASTM standards and are the reference format for bacteriological serology and cell wash procedures. MBP supplies Globe Scientific and NEST Scientific test tube lines for research and clinical labs across the USA, Canada, and worldwide. &nbsp; 
If you&rsquo;re choosing between glass and plastic or trying to match the right size to your workflow, it&rsquo;s worth getting that part right early &mdash; it makes everything downstream smoother and a lot less messy. <a href="https://mbpinc.net/contact-us">Contact the MBP team now!</a>

5527-5528-Group-Analyzer Consumables Catalog

5527-5528-Group-TDS-5527-5528

False Bottom Tubes & Caps

5527-5528 by Globe Scientific is a matched pair of PP false bottom tubes (5527: 16×97 mm, 5 mL, threaded) and white screw caps (5528) for use as secondary barcoded sample tubes on automated analyzers. Compatible with Hitachi, Olympus, Beckman, Tosoh, Vitros, and other platforms. Tubes: 1,000/case. Caps sold separately.

Manufacture

Tube, False Bottom, 16 x 97mm (5mL), with Threads, PP, Natural, CS/1000

Tube, False Bottom, 16 x 97mm (5mL), with Threads, PP, Natural, CS/1500

Cap, Screw, for False Bottom Tubes with Threads, White, BAG/1000

Cap, Screw, for False Bottom Tubes with Threads, White, BAG/1500

Formaldehyde Control

<h2 data-section-id="ibpx2r" data-start="0" data-end="42">What Are Formaldehyde Control Products?</h2>
&nbsp;
Formaldehyde control products are workplace safety tools used to monitor and manage exposure to airborne formaldehyde in laboratory and healthcare environments. These products are commonly used in histology, pathology, anatomy, and research laboratories where formalin-based fixatives are routinely handled during specimen preparation and preservation.
Because formalin is widely used as a tissue fixative, monitoring employee exposure is an important part of laboratory safety programs. Formaldehyde control products help laboratories assess exposure levels, document monitoring activities, and support compliance with workplace safety requirements.
Laboratories that routinely use formalin should implement formaldehyde monitoring programs, with passive badge dosimeters being one of the most common methods for measuring employee exposure over a work shift.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Formalin Neutralizers for safe liquid-to-non-toxic conversion.
</li>
<li dir="ltr" aria-level="1">
Vapor Suppressant Pads for rapid absorption and odor capture.
</li>
<li dir="ltr" aria-level="1">
Decontamination Sprays to eliminate residual chemical traces.
</li>
<li dir="ltr" aria-level="1">
Spill Response Kits for immediate laboratory emergency management.
</li>
<li dir="ltr" aria-level="1">
Absorbent Granules designed for high-capacity chemical containment.
</li>
<li dir="ltr" aria-level="1">
Protective Bench Mats to trap drips before they spread.
</li>
</ul>
&nbsp;
<h2 data-section-id="y865ie" data-start="919" data-end="965">How to Choose Formaldehyde Control Products</h2>
&nbsp;
Passive Badges vs Real-Time Monitors
Passive dosimeter badges are designed to measure employee exposure over an extended sampling period, typically a full work shift. These badges are worn by personnel and provide time-weighted average (TWA) exposure data that can be used for workplace monitoring and documentation.
Real-time monitors provide immediate readings of airborne formaldehyde concentrations. They are useful for investigating ventilation issues, evaluating work areas, and assessing conditions after spills or other incidents. However, they are generally used as supplemental monitoring tools rather than replacements for long-term exposure measurements.
Detection Range
When selecting a monitoring product, it is important to ensure that the detection range is appropriate for the expected workplace environment. Products designed for laboratory use typically measure concentrations across ranges relevant to occupational exposure monitoring programs.
Monitoring Frequency
Routine monitoring schedules help laboratories track exposure levels over time. Additional monitoring may be appropriate when laboratory procedures, equipment, staffing, ventilation systems, or formalin usage patterns change significantly.
Personnel Monitoring
Employees who regularly handle formalin-containing materials or work in areas where formaldehyde may be present are commonly included in monitoring programs. Monitoring helps laboratories evaluate workplace conditions and identify opportunities for improving safety controls.
Documentation and Recordkeeping
Accurate recordkeeping is an important component of formaldehyde monitoring programs. Laboratories often maintain records that include monitoring dates, employee assignments, badge identification numbers, sampling locations, and analytical results. Certificates of analysis and related documentation may also be retained as part of quality and compliance programs.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3575" data-end="3600">Specifications Context</h2>
&nbsp;
Formaldehyde monitoring products are widely used in histology and pathology laboratories where formalin-based tissue fixation is performed. Passive monitoring systems commonly use chemically treated collection media to capture airborne formaldehyde during the sampling period, followed by laboratory analysis.
Workplace exposure monitoring programs help laboratories evaluate ventilation effectiveness, document employee exposure levels, and support occupational safety initiatives. Many laboratories incorporate formaldehyde monitoring into their broader environmental health and safety programs.
Tired of harsh fumes slowing down your team? Upgrade to a safer lab environment; browse our inventory now and<a href="https://mbpinc.net/contact-us"> hit up the MBP team</a> for a quote.

Fan Pads, Formaldehyde Control

2543 by Globe Scientific is a large-format Formaldehyde Control Fan Pad (9&Prime; &times; 21&Prime;), sold in cases of 30, treated with Formalex liquid agent to eliminate formaldehyde, formalin, and glutaraldehyde vapors on contact. Used in pathology, histology, and gross dissection settings to absorb fluids and suppress hazardous aldehyde vapors at the work surface.

Formaldehyde Control, Fan Pad, Large, 9" x 21", 30/Unit

Formaldehyde Control, Fan Pad, Mini, 11" x 10-3/4", 55 Sheets/Roll, 6 Rolls/Unit

Formaldehyde Control, Fan Pad, Small, 6" x 14", 48/Unit

Formaldehyde Control, Fan Pad-GL Ultra, 8 x 11", 25/Pack, 4 Packs/Unit

Formaldehyde Control, Fad Pad-GL Ultra, 11 x 15", 25/Pack, 4 Packs/Unit

<h2>What are PCR and molecular assays for microbiomics?</h2>
&nbsp;
PCR and molecular assays in this category use quantitative PCR (qPCR) or digital PCR to measure the copy number of a defined DNA target, most often the 16S rRNA gene, to determine total bacterial load in a sample, or a specific pathogen or functional gene target for detection purposes. Unlike 16S or shotgun sequencing, which characterize the full taxonomic or functional composition of a community, these molecular assays provide a faster, more targeted quantitative or presence/absence readout for a specific question.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Femto&trade; Quantification Kits: Highly sensitive systems engineered for the detection of human, bacterial, or fungal DNA at the femtogram level with negligible sequence bias.
</li>
<li dir="ltr" aria-level="1">
High-Fidelity qPCR Premixes: Tailored master mixes designed for effective amplification and enhanced nuclear signal localization in low-biomass samples.
</li>
<li dir="ltr" aria-level="1">
Certified DNA Standards: Reference materials tested for reproducibility and No Template Controls (NTC) designed to calibrate the linear dynamic range and avert background contamination.
</li>
<li dir="ltr" aria-level="1">
ZymoTaq&trade; Specialized Polymerases: Enzymes that are heat-activated and free of nucleases, crafted to minimize non-specific amplification and guarantee high specificity in workflows associated with CRISPR.
</li>
</ul>
&nbsp;
<h2>How to choose PCR and molecular assays</h2>
&nbsp;
Use total bacterial load qPCR to screen samples before sequencing
A qPCR assay targeting the 16S rRNA gene quantifies total bacterial content in a sample, providing a useful pre-sequencing check for low-biomass samples likely to produce poor amplification or unreliable sequencing results if processed without this screening step.
Decide between qPCR and digital PCR based on sensitivity needs
Digital PCR can offer higher sensitivity and more accurate quantification than standard qPCR, particularly for low-biomass samples at the limit of detection, though at the cost of longer processing time, generally a meaningful tradeoff only when standard qPCR proves insufficient.
Confirm your qPCR assay design addresses known technical pitfalls
Well-designed 16S qPCR assays address known issues like self-priming or mispriming of unintended targets, amplification bias from non-optimal primer coverage, and amplicon size constraints for compatibility with clinical or high-throughput qPCR platforms.
Use pathogen-specific or functional gene qPCR for targeted detection
Beyond total bacterial load, qPCR can target a specific pathogen's single-copy gene or a functional gene of interest, such as an antibiotic resistance gene, providing a faster, more targeted answer than waiting for full sequencing and bioinformatic analysis.
Plan for known PCR limitations in environmental and clinical samples
Standard qPCR can be limited by reliance on standard reference materials for quantification and is prone to inhibition from substances naturally present in environmental or clinical samples, such as heavy metals or organic matter, which digital PCR can sometimes overcome with improved robustness.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
A PCR-based total bacterial quantification assay can provide an exact calculation of bacterial abundance useful for assessing whether a sample is likely to yield positive amplification before committing it to full sequencing, helping avoid wasted sequencing runs on low-biomass samples. As of 2026, digital PCR continues to see increased adoption over standard qPCR specifically for low-biomass, environmental, or inhibitor-prone sample types, where its improved sensitivity and accuracy can outweigh its longer processing time.
<a href="https://mbpinc.net/contact-us">Reach out to the expert team at MBP Inc.</a> now to ask for a quote on the appropriate PCR and molecular assays for your laboratory.

PCR & Molecular Assays

Protocol

DataSheet

Femto™ Bacterial DNA Quantification Kit (100 Rxns.)

E2006 by Zymo Research — Femto™ Bacterial DNA Quantification Kit, 100 reactions, qPCR detection 20 fg to 20 ng bacterial DNA from 1 µL sample. 16S rRNA primers; selective in mixed non-bacterial backgrounds. Store at −20°C.

Detection Range

Equipment

Sample Source

Shipping Condition

Storage Condition

Femto™ Bacterial qPCR Premix (1.8 ml)

Femto™ Bacterial qPCR Premix (E2006-1) by Zymo Research — 1.8 mL, 100 reactions, targets 16S rRNA gene, detects as few as 20 fg bacterial DNA (5 copies E. coli), 20 fg–20 ng quantification range. Includes bacterial DNA standards 1–7 and NTC.

Femto™ Fungal DNA Quantification Kit (100 Rxns.)

E2007 by Zymo Research is the Femto™ Fungal DNA Quantification Kit (100 rxns) — detects as little as 20 fg of fungal DNA per reaction using SYTO® 9 and ITS1-F/ITS2-R primers. Detection range 20 fg to 20 ng. S. cerevisiae-derived 7-point standard curve. High fungal specificity; no cross-reactivity with bacterial, plant, or animal DNA.

Femto™ Fungal qPCR Premix (1.8 ml)

E2007-1 by Zymo Research — Femto Fungal qPCR Premix; 1.8 mL 2× premix; ITS-targeting primers; 20 fg–20 ng detection range; ≥2 copies S. cerevisiae; compatible with any qPCR instrument; store at –20°C.

Femto™ Human DNA Quantification Kit (100 Rxns.)

E2005 by Zymo Research is the Femto™ Human DNA Quantification Kit (100 rxns) — detects as little as 20 femtograms of human DNA per reaction using SYTO® 9 and LINE-1 primers. Detection range 20 fg to 20 ng. HeLa-derived 7-point standard curve. High specificity; no cross-reactivity with non-human DNA.

Femto™ Human qPCR Premix (1.8 ml)

E2005-1 by Zymo Research: 1.8 ml 2× qPCR premix for human DNA quantification from 20 fg to 20 ng per reaction (&lt;1 copy of human genomic DNA). Targets LINE-1 on SYBR Green channel. Compatible with any real-time qPCR instrument. Store at −20°C.

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
&nbsp;
Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
&nbsp;
<h2 data-path-to-node="5">What you will find:</h2>
&nbsp;
<ul data-path-to-node="6">
<li style="font-family: arial, helvetica, sans-serif;">
Tissue-specific primary cell culture medium kits
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Specialized growth media for endothelial and epithelial cells
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Differentiation media for progenitor and stem cells&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Serum-free bioprocessing and cryopreservation reagents
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Targeted fibroblast, smooth muscle, and myocyte growth kits
</li>
</ul>
&nbsp;
<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
&nbsp;
Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
&nbsp;
Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Fibroblast Cell Growth Medium Kit

Unit Quantity

Sterility

Appearance

Shipping Conditions

Disclaimer

<h2>What are L-glutamine and amino acid supplements?</h2>
&nbsp;
L-glutamine and amino acid supplements are cell culture additives used to support cellular metabolism, growth, viability, and biosynthesis. They provide nutrients that may become depleted during culture or are required in greater quantities than those supplied by basal media alone. These supplements are commonly added to media such as DMEM, RPMI 1640, MEM, and DMEM/F-12 to optimize cell growth and maintain healthy cultures.
L-glutamine is one of the most important supplements in mammalian cell culture because it serves as a major energy source and is required for protein synthesis, nucleotide production, and glutathione synthesis. However, because free L-glutamine is unstable in solution and gradually breaks down into ammonia and pyroglutamate, many laboratories use stabilized alternatives such as GlutaMAX (L-alanyl-L-glutamine) for extended culture applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>L-Glutamine stable formulations for sourcing of essential nitrogen</li>
<li>Non-Essential Amino Acids (NEAA) for growth media enhancement</li>
<li>Synthetic Polymer Coatings Poly-L-Ornithine and Poly-L-Lysine solutions for better cell attachment.</li>
<li>Extracellular Matrix Proteins used for cell adhesion and include high-purity Fibronectin and Vitronectin coating reagents</li>
<li>Adhesion Promotion Reagents for improving the surface properties of culture vessels.</li>
</ul>
&nbsp;
<h2>How to choose L-glutamine and amino acid supplements</h2>
&nbsp;
L-glutamine concentration and format
Most mammalian cell culture systems use L-glutamine concentrations between 2&ndash;6 mM, although requirements vary depending on the medium and cell type. It is commonly supplied as:
<ul>
<li>USP-grade powder for custom media preparation</li>
<li>200 mM liquid stock solutions for direct supplementation</li>
</ul>
Liquid stocks should be aliquoted and stored frozen to minimize repeated freeze-thaw cycles and preserve stability.
Understanding glutamine degradation
Free L-glutamine gradually breaks down in aqueous solution through a non-enzymatic process that produces ammonia and pyroglutamate. This degradation accelerates with increasing temperature, storage time, and exposure to common cell culture buffer systems.
Ammonia accumulation can negatively affect cell growth, viability, productivity, and experimental reproducibility, particularly during long culture periods without media replacement.
GlutaMAX and stable glutamine alternatives
GlutaMAX (L-alanyl-L-glutamine) is a stabilized dipeptide form of glutamine designed to resist degradation in culture media. Once taken up by cells, it is converted into usable glutamine while maintaining greater stability during incubation.
<ul>
<li>Reduced ammonia accumulation</li>
<li>Improved media stability</li>
<li>Better performance in long-term cultures</li>
<li>Less frequent media replacement requirements</li>
</ul>
For most applications, GlutaMAX can directly replace standard L-glutamine without protocol modifications.
MEM non-essential amino acids (NEAA)
NEAA supplements provide amino acids that cells can synthesize themselves but may benefit from receiving directly from the culture medium. Supplementation reduces the metabolic burden of amino acid production and often improves growth rates and cell viability.
NEAA solutions are typically supplied as 100X concentrates and diluted to 1X working concentration.
HT supplement
HT supplement contains hypoxanthine and thymidine and is commonly used following HAT selection during hybridoma production. It supports nucleotide synthesis through salvage pathways while cells recover from selection pressure.
MEM vitamin solution
Vitamin supplements provide additional growth-supporting micronutrients that may become limiting during prolonged culture or in demanding applications. These supplements are often used alongside amino acid supplementation to enhance culture performance.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The standard L-glutamine concentration used in many mammalian culture systems is approximately 2 mM, though actual requirements vary by medium formulation and cell type. Free L-glutamine remains stable as a dry powder and frozen stock solution but degrades progressively once added to liquid media.
Stabilized glutamine products such as GlutaMAX have become increasingly common because they maintain glutamine availability while minimizing ammonia accumulation during incubation. These formulations are particularly valuable in long-duration experiments and high-density culture systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get research-grade supplements for your lab.

L-Glutamine & Amino Acids

Fibronectin Coating Solution (1 mg/mL)

Usage

Microplate Reader Accessories

<h2>What Are Microplate Reader Accessories?</h2>
&nbsp;
Microplate reader accessories are supplementary components that extend detection range, maintain instrument performance, and support compliance in regulated workflows. They fall into four groups: optical accessories (filters, filter cubes, and polarizers for wavelength selection in fluorescence and absorbance detection); illumination accessories (replacement xenon flash lamps, deuterium lamps, or LED upgrade modules that provide the excitation light source); calibration and validation tools (absorbance validation plates with certified reference values, fluorescence uniformity plates, and luminescence performance standards used to verify reader performance); and automation and software accessories (reagent injectors for kinetic luminescence assays, plate stackers for unattended batch reading, and software module licenses for 21 CFR Part 11 compliance, spectral scanning, or advanced kinetics). Because accessory designs are manufacturer-specific, a filter block designed for one plate reader brand will not fit another; always order accessories using the instrument's model number as the primary specification parameter. Filter sets are the most commonly needed accessory for multi-mode readers; confirm the filter block format and thread size for your specific reader model before ordering, as filter cube dimensions vary significantly between manufacturers and generations.
&nbsp;
<h2 dir="ltr">What you will find: </h2>
<ul>
<li data-section-id="23xybn" data-start="25" data-end="117">Replacement filters and optical accessories for maintaining microplate reader performance.</li>
<li data-section-id="71w1ic" data-start="118" data-end="209">Calibration and validation tools to support accurate and reproducible assay measurements.</li>
<li data-section-id="6jcbpl" data-start="210" data-end="312">Microplates and consumables compatible with absorbance, fluorescence, and luminescence applications.</li>
<li data-section-id="y9aam2" data-start="313" data-end="396">Replacement components and maintenance accessories for routine instrument upkeep.</li>
<li data-section-id="qoq7y7" data-start="397" data-end="523" data-is-last-node="">Accessories designed to support ELISA, cell-based assays, nucleic acid quantification, and other microplate-based workflows.</li>
</ul>
&nbsp;
<h2>How to Choose Microplate Reader Accessories</h2>
&nbsp;
Filter sets for fluorescence and absorbance.
Fluorescence filter sets (excitation filter, dichroic mirror, emission filter) must match both the fluorophore's excitation and emission spectra and the filter cube housing format of the specific reader. Most microplate reader filters use standard 25 mm diameter circular filters; some readers use 12 mm micro-filters. Common fluorescence filter sets: FITC/SYBR Green (Ex 485/12 nm, Em 520/10 nm); DAPI/Hoechst (Ex 360/40 nm, Em 460/40 nm); TRITC/PI (Ex 544/10 nm, Em 620/10 nm); Cy5 (Ex 620/10 nm, Em 680/10 nm). For absorbance, filter-based readers require individual narrow-bandpass filters at the wavelengths required by each assay protocol: 450 nm for ELISA, 595 nm for Bradford, 562 nm for BCA, 260 nm, and 280 nm for nucleic acid and protein.
Replacement lamps
Xenon flash lamps are the standard excitation source in high-quality fluorescence and absorbance microplate readers; typical lamp lifetime is 500 million flashes (approximately 3 to 5 years of daily use). Lamp output decreases gradually before failure; rising CV% in repeated measurements of the same standard is the first sign of lamp degradation. Replacement xenon flash lamps are model-specific; confirm part number from the instrument manual before ordering. Some instrument manufacturers have transitioned to LED-based excitation sources, which have much longer lifetimes (50,000+ hours) and do not require routine lamp replacement.
Calibration and validation plates
Calibration plates contain certified optical standards (neutral density filters, fluorescent reference dyes at known concentrations, or luminescent reference materials) in an SBS-format plate footprint for installation in the plate carriage. They are used to verify photometric accuracy, linearity, and inter-well uniformity (CV% across all 96 or 384 wells). These verifications are required in ISO 17025 accredited labs and GLP/GMP environments, and recommended as best practice for any lab relying on quantitative plate reader data. Validation plates are model-specific and must be re-certified periodically (typically annually) by the manufacturer.
Reagent injectors
Reagent injectors are syringe-pump accessories that dispense a defined volume of reagent into each well of a microplate immediately before or during reading, required for luminescence kinetic assays (luciferase, beta-galactosidase, flash luminescence from coelenterazine), where the signal decays rapidly after reagent addition. Single injectors add one reagent (e.g., luciferase substrate); dual injectors allow sequential addition of two reagents for sequential flash and glow luminescence detection. Injectors are model-specific and must be installed and calibrated by the manufacturer or a qualified service engineer.
Plate stackers and automation accessories
Plate stacker modules load and unload microplates automatically from a tray of 10 to 50 plates, allowing unattended overnight batch reading. Integration with laboratory robotics and LIMS extends this to fully automated plate reading workflows. Plate stackers are model-specific and typically sold as separate accessories from the reader. Confirm that the plate stacker is compatible with the specific plate type (96-well standard, 384-well, with or without sealing film) and the plate loading orientation.
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<h2>Specifications Context</h2>
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Filter bandpass width (the FWHM of the filter transmission curve) affects sensitivity and selectivity. Narrower bandpass filters (10 nm FWHM) improve selectivity by excluding off-target fluorophore emission but transmit less light, reducing signal. Wider bandpass filters (20 to 40 nm) transmit more light for a brighter signal, but may allow bleed-through from closely overlapping fluorophores. In multiplex assays with two or more fluorophores, narrow bandpass emission filters reduce spectral cross-talk between channels. Optical density (OD) of the blocking region outside the transmission band should be at least OD 5 to OD 6 for fluorescence filters to prevent light leakage from the excitation source from reaching the detector.
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To discuss product availability or get selection guidance,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Filter for Accuris Microplate Reader, 340 nm

Filter for Accuris Microplate Reader, 370 nm

Filter for Accuris Microplate Reader, 380 nm

Filter for Accuris Microplate Reader, 405 nm

Filter for Accuris Microplate Reader, 415 nm

Filter for Accuris Microplate Reader, 420 nm

Filter for Accuris Microplate Reader, 450 nm

Filter for Accuris Microplate Reader, 470 nm

Filter for Accuris Microplate Reader, 492 nm

Filter for Accuris Microplate Reader, 510 nm

Filter for Accuris Microplate Reader, 520 nm

Filter for Accuris Microplate Reader, 532 nm

Filter for Accuris Microplate Reader, 540 nm

Filter for Accuris Microplate Reader, 546 nm

Filter for Accuris Microplate Reader, 560 nm

Filter for Accuris Microplate Reader, 562 nm

Filter for Accuris Microplate Reader, 570 nm

Filter for Accuris Microplate Reader, 578 nm

Filter for Accuris Microplate Reader, 590 nm

Filter for Accuris Microplate Reader, 595 nm

Filter for Accuris Microplate Reader, 600 nm

Filter for Accuris Microplate Reader, 620 nm

Filter for Accuris Microplate Reader, 630 nm

Filter for Accuris Microplate Reader, 650 nm

Filter for Accuris Microplate Reader, 663 nm

Filter for Accuris Microplate Reader, 665 nm

Filter for Accuris Microplate Reader, 680 nm

Filter for Accuris Microplate Reader, 690 nm

Filter for Accuris Microplate Reader, 700 nm

Filter for Accuris Microplate Reader, 750 nm

Calibration plate for MR9600 SmartReader 96

Accuris Microplate Reader Filters

Accuris SmartReader 96, models MR9600 and MR9600-T

Filter for Accuris Microplate Reader

The MR9600-FLT by Accuris (Benchmark Scientific) is a narrowband interference filter for the Accuris SmartReader 96 MR9600 microplate absorbance reader. Available across 26 wavelengths from 340 to 750 nm—including 405, 450, 492, 562, 595, and 630 nm—each filter loads directly into the MR9600 8-position filter wheel to support ELISA, BCA, Bradford, MTS, and other colorimetric assays.