Zymo Research

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

MSDS

Quick-cfDNA/cfRNA™ Digestion Buffer (150 ml)

Quick-cfDNA/cfRNA™ Digestion Buffer (R1072-1-150) by Zymo Research is a 150 ml proteinase K digestion buffer used at a 1:1 ratio with serum or plasma in the Quick-cfDNA/cfRNA Serum & Plasma Kit (R1072). Applied at 37°C for 2 hours, it prepares liquid biopsy samples for cfDNA and cfRNA column-based purification.

Manufacturer

Shipping Condition

Storage Condition

<h2>What are Cell-Free DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Cell-free DNA/RNA co-extraction kits simultaneously isolate circulating cell-free DNA (cfDNA -- ctDNA fragments of 130-250 bp, apoptotic DNA) and cell-free RNA (cfRNA -- microRNAs, lncRNAs, exosomal RNA, circulating mRNA fragments) from the same plasma, serum, or urine sample without phenol-chloroform or protein precipitation, delivering both analytes for parallel liquid biopsy genomic and transcriptomic analysis from a single 1-4 ml blood draw. Unlike dedicated cfDNA kits that miss circulating RNA species, or cfRNA kits that under-recover short cfDNA fragments, co-extraction kits are formulated to bind both nucleic acid classes - including protein-bound RNA, exosomal RNA, and miRNA - from cell-free matrices where total nucleic acid concentration is 1-100 ng/ml. Use a cfDNA/cfRNA co-extraction kit when your liquid biopsy workflow requires both somatic mutation analysis (ctDNA sequencing) and RNA expression profiling (circulating miRNA or cfRNA-seq) from the same blood draw, or when sample volume is limited and splitting between separate DNA and RNA kits would leave insufficient input for either.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-cfDNA/cfRNA&trade; Serum &amp; Plasma Kit: An efficient spin-column setup tailored for sample volumes up to 3 ml, providing reduced sequence bias and recovering as much as 515 times more microRNA.
</li>
<li dir="ltr" aria-level="1">
High-Purity Elution Technology: Nuclease-free methods enabling the separate or co-elution of nucleic acids, ensuring transfection-grade purity for delicate bioinformatics and RT-qPCR uses.
</li>
</ul>
&nbsp;
<h2>How to Choose a cfDNA/cfRNA Co-Extraction Kit</h2>
&nbsp;
Input Volume and Assay Sensitivity
cfDNA concentration in cancer patient plasma ranges from 1-1,000 ng/ml depending on tumor stage and type; circulating miRNA concentration is typically 1-100 pg/ml. For low-MAF ctDNA detection (&lt;0.1%), 2-4 ml plasma input is required to obtain sufficient copies for ddPCR or ultra-deep sequencing. For cfRNA profiling only, 200 &mu;L-1 mL is sufficient for miRNA RT-qPCR panels. The Zymo Quick-cfDNA/cfRNA Kit scales linearly up to 3 ml input.
Co-Elution vs. Separate Fractions
The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit offers the option to co-elute DNA and RNA into a single fraction or to collect separate fractions. Co-elution maximizes nucleic acid recovery in the smallest volume -- optimal for low-abundance targets; separate fractions are needed when DNA-specific (bisulfite sequencing, whole-exome) and RNA-specific (rRNA depletion, miRNA-seq library) downstream protocols cannot use the same input.
miRNA Recovery
Circulating miRNAs (18-22 nt) are small, low-abundance, and easily lost during standard DNA extraction. Confirm the co-extraction kit explicitly recovers small RNAs &lt;200 nt from plasma. The Zymo Quick-cfDNA/cfRNA Kit recovers up to 515x more microRNA than conventional phase-separation methods -- a validated difference confirmed in the Zymo product datasheet.
Phenol-Free Chemistry
For high-throughput liquid biopsy programs, phenol-free kits eliminate fume-hood requirements and toxic waste handling. The QIAGEN ccfDNA/RNA Kit and Zymo Quick-cfDNA/cfRNA Kit are both phenol-free; automated protocols using these kits can process large cohort batches without hazardous chemical handling steps.
Pre-Analytical Standardization
cfDNA/cfRNA concentrations vary with blood collection tube type, time-to-processing, and centrifugation protocol. Standardize on EDTA tubes + two-spin plasma preparation (300xg then 16,000xg) and process within 6 hours of collection or store at -80 degrees C. For samples requiring extended transport, Streck Cell-Free DNA BCT or cfRNA preservation tubes stabilize both analytes at room temperature for up to 7 days before extraction.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit enables simple spin-column isolation of all circulating cell-free DNA and RNA from serum, plasma, and biological fluids, recovering protein-bound, exosomal, miRNA, and other small RNA in addition to cfDNA. Cell-free nucleic acids scale linearly with input volume up to 3 ml. A 2022 digital PCR comparative study (PMC9805675) evaluated multiple cfDNA/cfRNA co-purification kits across plasma input volumes of 0.06-4 ml, finding that combined cfDNA/cfRNA quantification produces higher total nucleic acid concentrations than parallel separate quantification. cfDNA/cfRNA multi-omic liquid biopsy is an active area of clinical translational research for non-invasive cancer diagnosis and treatment monitoring, with studies underway at MD Anderson Cancer Center and Vanderbilt University using co-extraction formats to maximize information per blood draw.
&nbsp;
Close the gap between liquid biopsy and groundbreaking discovery&mdash;<a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> now to obtain a quote for your next-generation sequencing requirements.

Cell-free DNA/RNA

DataSheet

Protocol

Quick-cfDNA/cfRNA™ Serum & Plasma Kit (50 Preps.)

Quick-cfDNA/cfRNA™ Serum &amp; Plasma Kit (R1072) by Zymo Research — 50 preps. Isolates cfDNA (≥50 bp) and cfRNA/miRNA (≥17 nt) from up to 3 ml serum, plasma, CSF, urine, or biological fluids. Elution 6–15 µl. Up to 515× more microRNA recovery. Co-purification (~30 min) or parallel purification modes.

Elution Volume

Processing Time

Purity

Required Equipment

Sample Types

Size

Yield

<h2>What Are Cell-Free DNA Extraction Kits?</h2>
&nbsp;
A cell-free DNA (cfDNA) extraction kit purifies circulating cfDNA fragments from plasma, serum, or urine using silica spin-column or magnetic bead-based technologies. These kits are optimized to recover the short DNA fragments commonly found in liquid biopsy samples for downstream applications such as PCR, qPCR, next-generation sequencing (NGS), and methylation analysis. Choose a cfDNA-specific kit over a general genomic DNA kit whenever short circulating DNA fragments are the primary target.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>MagBio cfKapture ccfDNA Extraction Kit: for cell-free DNA isolation using advanced magnetic bead-based technology.</li>
<li>MAGicBead&trade; cfDNA Isolation Kit: for automated, high-throughput liquid biopsy workflows using high-binding paramagnetic particles.</li>
<li>Quick-cfDNA&trade; Serum &amp; Plasma Kit: for streamlined manual benchtop purification using rapid spin-column technology.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free DNA Extraction Kit</h2>
&nbsp;
Input Volume Range
Choose a kit compatible with your typical plasma, serum, or urine input volume. Larger sample volumes generally increase total cfDNA recovery but may require additional reagents and longer processing times.
Column-Based vs. Magnetic Bead-Based Chemistry
Silica spin-column kits are widely used for manual purification workflows, while magnetic bead-based kits simplify automation and high-throughput processing on compatible liquid handling platforms.
Elution Volume for Downstream Assays
Select an elution volume that matches the input requirements of your downstream applications, such as PCR, qPCR, or NGS library preparation, to minimize additional concentration or dilution steps.
Combined DNA/RNA Recovery
If your workflow requires analysis of both circulating DNA and RNA from the same specimen, consider kits designed to recover multiple nucleic acid types from a single sample.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Key considerations when selecting a cell-free DNA extraction kit include sample input volume, purification chemistry, compatibility with manual or automated workflows, and elution volume for downstream molecular assays. Magnetic bead-based systems are commonly selected for scalable, automated liquid biopsy workflows, while spin-column kits provide reliable manual purification for routine laboratory use. The optimal kit depends on your sample type, throughput requirements, and downstream analytical methods.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Cell-Free DNA

D4076-Data Sheet

D4076 - MSDS

D4076-1-32 - MSDS

D4076-2-170 - MSDS

D4076-3-10 - MSDS

D4076-4-12 - MSDS

D4076-A - MSDS

D4076-Protocol

Quick-cfDNA™ Serum & Plasma Kit

Quick-cfDNA™ Serum &amp; Plasma Kit (D4076) by Zymo Research — 50 preps, purifies cfDNA ≥100 bp from up to 10 ml serum/plasma. Also compatible with amniotic fluid, CSF, and saliva. Elution ≥35 µl. Compatible with Streck and PAXgene tubes. Individual buffers and a bundled buffer set also sold separately.

DNA Purity

DNA Recovery

DNA Storage

Processing volume

Recovery Volume

Sample Sources

Quick-cfDNA™ Serum & Plasma Kit (50 Preps.)

S&P 5X Digestion Buffer (32 ml)

S&P DNA Binding Buffer (170 ml)

S&P DNA Prep Buffer (10 ml)

S&P DNA Wash Buffer (Concentrate) (12 ml)

Quick-cfDNA™ Serum & Plasma Buffer Set

Quick-cfRNA™ Binding Buffer (100 ml)

R1059-4-100 by Zymo Research is the Quick-cfRNA&trade; Binding Buffer supplied in a 100 ml volume for cell-free RNA (cfRNA) isolation from serum, plasma, cerebrospinal fluid, and urine. Added at a 1:1 ratio to the proteinase K-digested sample, this buffer promotes RNA adsorption onto Zymo-Spin IC Columns and enables recovery of cfRNA fragments &ge;17 nucleotides in length, including miRNA and exosomal RNA.
Storage is at room temperature. The buffer is a standalone refill component for the Quick-cfRNA Serum &amp; Plasma Kit (R1059) and is not interchangeable with the Quick-cfDNA/cfRNA Binding Buffer (R1072-2-150), which serves a different dual-extraction kit.
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Place your order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-cfRNA™ Digestion Buffer (50 ml)

Quick-cfRNA™ Digestion Buffer R1059-3-50 by Zymo Research — 50 ml, room-temperature stable — digests proteins prior to cell-free RNA isolation from serum and plasma. Use 200 µL per 200 µL sample with Proteinase K (37°C, 2 hours); Streck Blood Tube samples require an additional 55°C pre-digestion step. Available from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

<h2>What are Cell-Free RNA Extraction Kits?</h2>
&nbsp;
Cell-free RNA (cfRNA) extraction kits isolate circulating RNA molecules - microRNAs, long non-coding RNAs, exosomal RNA, and mRNA fragments - from blood plasma, serum, urine, cerebrospinal fluid, and saliva, where endogenous RNA concentrations range from 1-100 ng/ml. Unlike standard total RNA kits designed for cell lysates containing micrograms of intracellular RNA per sample, cell-free RNA kits are built for the low-abundance, fragmented, and often vesicle-packaged RNA species present in acellular body fluids. They incorporate carrier RNA to prevent adsorption loss, handle input volumes of 200 ul to 5 mL, and use binding chemistries optimized for small RNAs &lt;200 nt. Use a cell-free RNA kit - not a standard total RNA or viral RNA kit - whenever your input is plasma, serum, urine, or another cell-depleted biofluid and your target is circulating miRNA, cfRNA, or exosomal RNA.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1fsegxt" data-start="25" data-end="105">Rapid purification of circulating cell-free RNA (cfRNA) from plasma and serum.</li>
<li data-section-id="dahqiz" data-start="106" data-end="181">Efficient recovery of low-abundance cell-free RNA from biological fluids.</li>
<li data-section-id="85g4hb" data-start="182" data-end="245">Zymo-Spin&trade; spin-column technology for reliable RNA isolation.</li>
<li data-section-id="exy4np" data-start="246" data-end="296">Streamlined workflow for fast sample processing.</li>
<li data-section-id="mtq4uw" data-start="297" data-end="369" data-is-last-node="">Purified cfRNA suitable for downstream molecular biology applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Cell-Free RNA Extraction Kit</h2>
&nbsp;
Input Volume and Throughput
Standard cell-free RNA kits accept 200 &mu;L-1 mL of plasma or serum per extraction; this input volume is appropriate for RT-qPCR quantification of abundant miRNA species and exosomal RNA panels. For discovery-phase cfRNA-seq, where rare transcripts are targets, use high-volume extraction formats to increase RNA mass from low-abundance circulating species.
Small RNA Enrichment
Circulating miRNAs are the most studied cfRNA analytes; kits that enrich for RNA &lt;200 nt deliver higher sensitivity for miRNA panels than kits recovering large and small RNAs together.&nbsp;
Carrier RNA and Spike-In Controls
Every cell-free RNA extraction should include both a carrier RNA (added to the lysis buffer to improve recovery) and an exogenous spike-in control RNA - typically Caenorhabditis elegans miR-39 (cel-miR-39) at 1.6 &times; 10^8 copies per extraction - added at the lysis step. Spike-ins normalize inter-sample variation in extraction efficiency and enable detection of failed extractions. Confirm whether carrier RNA is supplied in the kit or purchased separately.
Magnetic Bead vs. Spin-Column Format
Spin-column cfRNA kits (Zymo Quick-RNA Serum &amp; Plasma) are the standard for 1-24 samples per session and require only a microcentrifuge. Magnetic bead formats are preferred for higher-volume inputs and automation, and demonstrate superior recovery of fragmented low-abundance RNA from dilute matrices in published comparative studies.
Phenol-Free vs. Phenol-Containing Lysis
Most current cell-free RNA kits use guanidinium-based, phenol-free lysis buffers, avoiding the hazardous handling requirements of phenol-containing reagents. Phenol-chloroform-based extraction of cfRNA is used in research settings for the highest yield but introduces extraction bias for certain miRNA species and requires additional purification steps; column-based phenol-free kits are preferred for clinical and high-throughput biomarker applications.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for cell-free RNA extraction: recovery of cel-miR-39 spike-in within 2 Ct of the expected value, A260/A280 &gt;= 1.7 (lower values are acceptable for extremely dilute cfRNA samples), and consistent inter-sample Ct variation &lt;1.5 Ct for reference miRNAs across a batch. Circulating miRNA biomarkers -- including miR-21, miR-155, miR-210, miR-126, and miR-200 family members - are under clinical evaluation as non-invasive cancer, cardiovascular, and neurological disease biomarkers, with studies published by groups at institutions including MD Anderson Cancer Center and Vanderbilt University. Pre-analytical variables -- plasma preparation method, freeze-thaw cycles, hemolysis - are major sources of inter-study variability in cfRNA data; standardized two-spin plasma preparation protocols are recommended before extraction.
&nbsp;
For ordering assistance, bulk pricing, or protocol guidance on selecting the right kit for your sample type, <a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

Cell-free RNA

White Paper

Quick-cfRNA™ Serum and Plasma (50 Preps.)

Quick-cfRNA™ Serum and Plasma Kit (R1059) by Zymo Research isolates cell-free RNA (≥17 nt, including microRNA) from up to 3 mL of plasma, serum, CSF, amniotic fluid, or urine in a spin-column workflow with 6–15 µl concentrated elution. No phenol-chloroform required. 50-prep format for liquid biopsy and cfRNA biomarker research.

Sample Type

<h2>What Are 96-Well Plate Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
96-well plate genomic DNA kits for blood and tissue replace individual spin columns with silica-binding or glass-fiber plates that enable simultaneous purification of genomic, mitochondrial, or pathogen DNA from up to 96 samples per run. These systems are designed for whole blood, serum, plasma, lymphocytes, cultured cells, and selected tissue samples, providing efficient high-throughput DNA extraction for research and molecular testing workflows.&nbsp;Choose a 96-well plate genomic DNA kit when sample volumes justify batch processing on a plate-compatible centrifuge or vacuum manifold.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Streamlined high-throughput processing using 96-well plate formats for simultaneous genomic DNA purification.</li>
<li>High-purity DNA extraction from blood and tissue samples using optimized purification chemistries.</li>
<li>Compatibility with both automated and manual workflows for scalable sample preparation in research and diagnostic laboratories.</li>
<li>Purified DNA suitable for sequencing, PCR, qPCR, genotyping, and other downstream molecular biology applications.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Blood and Tissue Genomic DNA Kit</h2>
&nbsp;
Sample Input and Well Capacity
Most kits accept up to 200 &micro;L of whole blood, serum, plasma, or approximately 5 &times; 106 cultured cells per well. Exceeding recommended input volumes can reduce DNA binding efficiency and overall recovery.
Centrifuge Rotor vs. Vacuum Manifold Compatibility
96-well plate kits typically require either a centrifuge equipped with a compatible 96-well plate rotor and deep bucket carriers or a vacuum manifold system. If your laboratory lacks a plate-compatible centrifuge, confirm vacuum manifold compatibility before selecting a kit.
Yield and Elution Volume
A typical extraction yields approximately 6 &micro;g of genomic DNA from 200 &micro;L of whole blood, with elution volumes commonly around 200 &micro;L using TE buffer or nuclease-free water. Processing times generally range from 45 minutes to a few hours, depending on workflow design.
Throughput and Sample Type Considerations
These kits are optimized for high-throughput processing and can handle large sample batches efficiently. When processing multiple sample types within the same plate, additional pretreatment steps may be required for certain tissues or cell types.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The key specifications for 96-well plate genomic DNA extraction kits include sample input volume, DNA yield, elution volume, and compatibility with centrifuge-based or vacuum-manifold workflows. Most kits in this category are designed for high-throughput purification of genomic DNA from blood, tissue, and cultured cell samples while maintaining consistent recovery across large sample batches.&nbsp;Products such as the Quick-DNA 96 Kit, Quick-DNA 96 Plus Kit, 96-well Blood DNA Kit, and 96-well Tissue DNA Kit support parallel processing in a plate-based format, helping laboratories scale routine genotyping, PCR, qPCR, and sequencing workflows. Typical workflows combine optimized lysis and silica-based DNA binding chemistry to remove inhibitors while producing purified DNA suitable for downstream molecular biology applications.&nbsp;When selecting a kit, confirm compatibility with your laboratory's centrifuge rotor, 96-well carrier, or vacuum manifold system, as equipment requirements vary between platforms. Most kit components are stored at room temperature, while any included enzyme reagents should be stored according to the manufacturer's recommendations. Lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>today to discuss high-throughput genomic DNA extraction workflows and receive expert guidance on selecting the right 96-well plate solution for your laboratory.

96-well plate

Quick-DNA 96 Kit

The Quick-DNA 96 Kit by Zymo Research is a high-throughput 96-well genomic DNA purification kit (≤5 µg/well, ≥40 kb, A260/A280 &gt;1.8) from blood, cells, and tissue; compatible with PCR, genotyping, bisulfite conversion, and NGS. Available in 2×96 (D3010), 4×96 (D3011), and 10×96 (D3012) prep formats.

ZR-96 Quick-DNA™ Kit (2 x 96 Preps.)

ZR-96 Quick-DNA™ (4 x 96 Preps.)

ZR-96 Quick-DNA™ (10 x 96 Preps.)

D4070-Data Sheet

D4070 - MSDS

D4070-Protocol

Quick-DNA 96 Plus Kit

D4070 by Zymo Research: Quick-DNA™ 96 Plus Kit, 2×96 preps. Extracts genomic DNA from biofluids, cells, and solid tissues. 5 µg/well binding capacity; ≥15 µl elution; Proteinase K digestion at 55°C. Typical yield: 5–7 µg from 100 µl whole blood. Made in USA.

Equipment

Size Range

Workflow

Quick-DNA™ 96 Plus Kit (2 x 96 Preps.)

Quick-DNA™ 96 Plus Kit (4 x 96 Preps.)

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

Quick-DNA Fecal/Soil Microbe MicroPrep Kit (50 Preps.)

D6012 Quick-DNA Fecal/Soil Microbe MicroPrep Kit by Zymo Research — 50 preps, ≤15 min, ≤50 mg feces or ≤250 mg soil input, inhibitor-free DNA, ≤5 µg yield, ≥20 µl elution.

Applicable For

Processing Volume

Sample Source

Sample Storage

Type

D4077-1-MSDS

Quick-DNA MagBinding Buffer

Zymo Research Quick-DNA™ MagBinding Buffer — binding buffer for Zymo Quick-DNA MagBead DNA purification kits. Two volumes: D4077-1-150 (150 mL) and D4077-1-250 (250 mL). Standalone component for replenishment or throughput scale-up.

Quick-DNA™ MagBinding Buffer (150 ml)

Quick-DNA™ MagBinding Buffer (250 ml)

<h2>What Are Spin-Column Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
A spin-column genomic DNA kit for blood and tissue combines a proteinase K-based direct lysis step with a silica-membrane spin column to purify genomic, mitochondrial, or viral DNA from animal blood, tissue, and cultured cells without phenol, chloroform, or alcohol precipitation. Related entities include direct-lysis protocols, dried blood spot supplementary workflows, TissueLyser mechanical disruption, and downstream PCR, genotyping, and sequencing library preparation.&nbsp;Use spin-column formats for individual or small-batch genomic DNA extraction from blood, cells, or tissue using a standard microcentrifuge.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Efficient isolation of high-quality genomic DNA from blood, saliva, tissues, and other biological samples using rapid spin-column purification.&nbsp;</li>
<li>Maximize DNA recovery with optimized lysis and binding chemistry while minimizing inhibitor, protein, and contaminant interference</li>
<li>Midiprep, microprep, and miniprep formats available to support varied sample input volumes&nbsp;</li>
<li>User-friendly workflows and streamlined protocols with fast processing times for efficient genomic DNA extraction&nbsp;</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Blood/Tissue Genomic DNA Kit</h2>
&nbsp;
Lysis Approach by Sample Type
Whole blood, buffy coat, buccal cells, and cultured cells generally undergo direct proteinase K lysis without mechanical disruption. In contrast, fibrous or fatty tissues such as muscle, liver, or skin often require mechanical homogenization prior to the lysis step to ensure complete cell disruption.
Anticoagulant and Blood Sample Handling
These kits typically support fresh or frozen blood collected in EDTA, citrate, or heparin. Dried blood spots generally require supplementary protocols and additional equipment beyond standard spin-column workflows.
Column Elution Chemistry
Elution is typically performed using Tris-based buffer or nuclease-free water, with volumes ranging from approximately 50&ndash;200 &micro;L depending on kit design and sample type. Water elution may be preferred for EDTA-sensitive downstream applications.
Scaling Beyond a Single Column
For higher throughput workflows, 96-well plate formats using the same silica-membrane chemistry enable simultaneous processing of multiple samples per centrifuge run. Spin-column formats remain best suited for method development and low-to-moderate sample volumes.
&nbsp;
<h2>Specifications Context:</h2>
&nbsp;
The most important parameters for spin-column blood/tissue genomic DNA kits are sample-type compatibility and elution buffer composition, as these determine whether additional pretreatment is required and whether the eluate is directly compatible with downstream workflows. A typical 200 &micro;L whole blood input yields low-microgram quantities of genomic DNA in an elution volume of approximately 50&ndash;200 &micro;L.
&nbsp;
Use MBP's <a href="mbpinc.net/quickorder">Quick Order tool</a> for bulk pricing or <a href="mbpinc.net/contact-us">contact the specialist team</a> to confirm pretreatment requirements for challenging tissue types.

Spin-column

Quick-DNA Microprep Kit

Zymo Research Quick-DNA™ MicroPrep Kit for genomic DNA isolation from low-cell-count and micro-input samples. Chaotropic lysis + Proteinase K + Zymo-Spin silica column, ≥10 µl elution. D3020 (50 preps) and D3021 (200 preps).

Quick-DNA™ MicroPrep (50 Preps.)

Quick-DNA™ MicroPrep (200 Preps.)

Quick-DNA Miniprep

The Quick-DNA™ Miniprep Kit by Zymo Research provides rapid, Proteinase K-free gDNA isolation from whole blood (up to 200 µl), plasma, serum, cultured cells, and buccal samples—yielding 3–7 µg per 100 µl blood (up to 25 µg total) with A260/A280 &gt;1.8, DNA up to 40 kb+, in ≥50 µl eluate. Available as D3024 (50 Preps.) and D3025 (200 Preps.). The inhibitor-free output is directly compatible with PCR, qPCR, restriction digestion, and bisulfite conversion.