Epigenetics

<h2>What are epigenetics NGS library preparation kits?</h2>
&nbsp;
Epigenetics NGS library preparation kits convert the DNA output of an upstream epigenetics workflow into an indexed sequencing library: adapters are ligated to the ends of each DNA fragment, an optional amplification step increases the total amount of library, and indexes are added to allow multiplexed sequencing of many samples in a single run. These kits are specifically optimized for epigenetics-specific inputs, including ChIP-enriched DNA (often low-yield, sheared to 200&ndash;750 bp), chromatin-accessible DNA from ATAC-seq (also low-yield and fragmented), and bisulfite-converted DNA (fragmented and chemically modified, with low-complexity sequence).
&nbsp;
<h2 dir="ltr">What You Will Discover:</h2>
&nbsp;
<ul>
<li dir="ltr">Groundbreaking single-tube processes that integrate bisulfite conversion and library preparation to chart entire genomes with extremely low input needs.</li>
<li dir="ltr">Rapid, efficient transposase-driven methods that seize open chromatin areas and dynamic regulatory environments in only a few hours.</li>
<li dir="ltr">Enzymatic restriction methods combined with targeted sequencing for affordable, high-density CpG coverage without incurring full-genome expenses.</li>
<li dir="ltr">Advanced multi-adapter ligation techniques designed to reduce bias and optimize library retrieval from fragmented or challenging DNA sources.</li>
<li dir="ltr">Post-bisulfite library preparation systems designed explicitly for sub-nanogram DNA quantities, individual cells, or extensively degraded specimens.</li>
<li dir="ltr">Locus-specific, genome-wide enrichment approaches aimed at accurately monitoring and characterizing 5-hydroxymethylcytosine alterations throughout the genome.</li>
</ul>
&nbsp;
<h2>How to choose epigenetics NGS library preparation kits</h2>
&nbsp;
Match your library prep kit to your specific epigenetics input type
ChIP-seq library prep kits are optimized for low-yield sheared chromatin enriched by immunoprecipitation, while ATAC-seq kits work with tagmented open chromatin fragments, and bisulfite or enzymatic methyl-seq library prep kits are specifically formulated for the highly fragmented, low-complexity sequence characteristics of bisulfite-converted DNA input.
Confirm the kit supports your input DNA amount
ChIP experiments, especially those targeting low-abundance transcription factors or run from limited cell numbers, often yield very little enriched DNA, and kits validated for low-input or picogram-scale library preparation are necessary to generate libraries with sufficient complexity without excessive PCR amplification introducing duplicates.
Choose an indexing strategy appropriate to your sample multiplexing plan
Dual-index strategies, using unique combinations of two distinct index sequences per sample, provide more reliable sample demultiplexing in highly multiplexed runs compared with single-index strategies, and confirming whether your kit supports the number of samples and multiplexing depth required is an important selection criterion.
Understand the impact of PCR cycles on library complexity and duplicate rate
Fewer PCR amplification cycles reduce the proportion of duplicate reads in sequencing data, which is especially important for low-complexity epigenetics libraries where overamplification can dominate output with copies of the same original fragment. Efficient ligation chemistry that increases library yield from low input helps minimize required PCR cycles.
Confirm kit compatibility with your downstream analysis pipeline
Some library prep protocols use specific adapter sequences or library structures that require corresponding adjustments in bioinformatics analysis pipelines, and confirming compatibility before sequencing helps avoid downstream analysis failures.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
ChIP-seq library prep kits optimized for low-input or ultra-low-input chromatin are designed to achieve high library complexity from picogram-scale DNA inputs that would otherwise produce low-complexity libraries with standard kits at similar PCR cycle numbers. As of 2026, library prep kits compatible with enzymatic methyl-seq (EM-seq) as well as classical bisulfite-converted DNA continue to expand, enabling researchers to leverage improved DNA quality while maintaining familiar downstream sequencing workflows.
Prepared to enhance your sequencing efficiency? Discover our complete selection of NGS Library Preparation Kits and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a quote now.

NGS Libray Preparation Kits

DataSheet

MSDS

Protocol

Zymo-Seq RRBS Library Kit

The D5460 by Zymo Research is a 24-prep all-in-one RRBS library prep kit for DNA methylation profiling at single-nucleotide resolution, accepting 10–500 ng genomic DNA input with ~2 hours hands-on time and ≥70% CpG island coverage. Includes Unique Dual Index (UDI) primers and is compatible with all Illumina platforms except HiSeq X Series.

Equipment Required

Input

Sample Sources

Sequencing Platform Compatible

Manufacturer

Shipping Condition

Storage Condition

Zymo-Seq RRBS™ Library Kit (24 Preps.)

Zymo-Seq RRBS™ Library Kit (48 Preps.)

Zymo-Seq SPLAT DNA Library Kit (12 Preps)

D5464 Zymo-Seq SPLAT DNA Library Kit by Zymo Research — 12 preps, 1–500 ng input, cfDNA, FFPE, and genomic DNA, all Illumina platforms, ~3 hours, 12 unique dual indexes.

Sample Input Type

Minimum Input

Maximum Input

Input Quality

Total Processing Time

Hands-On Time

Library Storage

Sequencing Platform Compatibility

Barcode Sequences

D5462 - MSDS

D5463 - MSDS

D5462-Protocol

Zymo-Seq Trio WGBS Library Kit

Zymo-Seq Trio WGBS Library Kit (D5462) by Zymo Research — 24-prep kit for whole genome bisulfite sequencing library preparation from 5–10 ng cfDNA, FFPE, or sheared genomic DNA. Greater than 99.5% bisulfite conversion efficiency; ~6 hours total processing; compatible with all Illumina platforms. Made in the USA.

Bisulfite Conversion

Sample Input Material

Zymo-Seq Trio WGBS Library Kit (24 Preps)

Zymo-Seq Trio WGBS Library Kit (96 Preps)

MSDS (CA)

MSDS (EU)

MSDS (US)

Zymo-Seq WGBS Library Kit (24 Rxns.)

D5465 by Zymo Research is the Zymo-Seq WGBS Library Kit (24 Rxns.), enabling whole genome bisulfite sequencing library preparation in a single tube in ~4 hours with 10–100 ng DNA input and &gt;90% CpG site coverage across mammalian genomes. Compatible with all Illumina sequencing platforms.

DNA Input

Processing Time

Required Equipment

Required material not provided

Sequencing Platform

<h2>What are ChIP kits?</h2>
&nbsp;
ChIP (chromatin immunoprecipitation) kits provide all the components needed to enrich a specific protein-DNA complex from fixed, sheared chromatin using an antibody against the protein of interest, whether a histone modification like H3K4me3 or a DNA-binding protein like a transcription factor. A complete ChIP kit covers: formaldehyde crosslinking of proteins to DNA in intact cells or tissue, cell lysis and nuclear extraction, chromatin shearing to appropriate fragment sizes, immunoprecipitation using an antibody pre-bound to protein A/G beads, sequential washing to remove non-specific binding, elution of the antibody-protein-DNA complex, de-crosslinking to release the DNA, and purification of the enriched DNA for downstream analysis.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/chip-kits/buffers">Buffers: </a>for immunoprecipitation, chromatin preparation, elution, and washing workflows. Includes Chromatin Shearing Buffer, MN Digestion Buffer, and Chromatin Wash Buffers, designed to support efficient chromatin cleanup, fragmentation, and recovery in ChIP assays
</li>
</ul>
&nbsp;
<h2>How to choose and use ChIP kits</h2>
&nbsp;
Decide between sonication-based and enzymatic chromatin shearing
Sonication shears chromatin mechanically into fragments typically 200&ndash;1000 bp through several optimized burst-and-cool cycles, while enzymatic kits use micrococcal nuclease (MNase) or similar enzymes to cleave chromatin at accessible linker regions. Each approach has different resolution characteristics and cell-type-specific optimization requirements.
Confirm your ChIP kit is compatible with your antibody and magnetic bead format
Most modern ChIP kits use magnetic protein A or protein G Dynabeads to capture the antibody-antigen complex during immunoprecipitation, and matching the bead type to the antibody species and isotype is important for efficient capture and low background.
Plan sonication optimization as a required step before your ChIP experiment
Fragment size after sonication, ideally 200&ndash;750 bp for most ChIP-seq applications, must be confirmed by gel electrophoresis or Bioanalyzer before proceeding, since fragment size directly affects both immunoprecipitation efficiency and the resolution of the resulting ChIP-seq data.
Include an input chromatin control and an isotype control antibody
Running an aliquot of the pre-immunoprecipitation chromatin as an input control normalizes for differences in chromatin accessibility across the genome, while an isotype-matched antibody control establishes background enrichment levels above which true binding sites can be distinguished.
Keep all steps after cell lysis cold to prevent protein degradation
Chromatin prepared for ChIP is sensitive to protease activity and non-specific interactions after formaldehyde fixation, and including protease inhibitors in all lysis and wash buffers, as well as keeping samples cold throughout, reduces background and preserves specificity of the immunoprecipitation.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Sonication requires careful optimization for each cell type and instrument, typically involving pilot experiments at varying pulse widths, total times, and rest intervals, with fragment size evaluated by gel electrophoresis after de-crosslinking to confirm the target range of 200&ndash;750 bp before a full ChIP experiment is run. As of 2026, low-input and ultra-low-input ChIP kits continue to expand access to ChIP experiments from limited cell numbers such as primary cell populations and rare clinical samples, while nano-ChIP and CUT&amp;RUN approaches provide complementary alternatives for very low cell inputs where classical ChIP requires too many cells.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) to get premium-quality ChIP reagents and buffers for your lab.

ChIP Kits

D5209 - MSDS

MSDS - D5210

Zymo-Spin™ ChIP Kit

Zymo-Spin ChIP Kit (D5209) by Zymo Research — 10-prep chromatin immunoprecipitation kit for mammalian cells. Combines ZymoMag Protein A bead capture with Zymo-Spin micro-elution columns (≥6 µl recovery). Suitable for ChIP-qPCR and ChIP-Seq. Made in the USA.

Applicable For

Sample Source

Storage

Zymo-Spin™ ChIP Kit (10 Preps.)

Zymo-Spin™ ChIP Kit (25 Preps.)

<h2>What are epigenetics dNTPs?</h2>
&nbsp;
Epigenetics dNTPs includes both standard deoxynucleotide triphosphate sets used across many molecular biology applications and modified nucleotide analogs relevant specifically to epigenetic research, with the most important being 5-methyl-dCTP, a base-modified analog in which the cytosine base carries a methyl group at the 5-carbon position. 5-methyl-dCTP can be incorporated into DNA via PCR or in vitro synthesis in place of standard dCTP, producing a defined, fully methylated cytosine-substituted DNA molecule useful as a positive control for methylation detection assays or as a substrate for studying methylation-related enzyme activity.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li><a href="https://mbpinc.net/reagents/epigenetics/dntps-deoxynucleotide-triphosphates/methylated">Methylated: </a>dNTP mixes used in methylation-sensitive DNA synthesis workflows.&nbsp;Methylated nucleotide analogs for DNA methylation studies and epigenetics research.&nbsp;Standard individual dNTPs (dATP, dCTP, dGTP, dTTP) for PCR analysis and routine DNA synthesis.</li>
</ul>
&nbsp;
<h2>How to choose epigenetics dNTPs</h2>
&nbsp;
Use 5-methyl-dCTP to build defined, fully methylated control DNA
Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction produces a fully methylated cytosine-substituted amplicon, providing a positive control whose methylation status is defined and known, unlike a positive control derived from genomic DNA with variable, unknown methylation patterns.
Confirm that your methylation detection assay is validated with a defined methylated control
Running a fully methylated positive control through a bisulfite conversion or enzymatic conversion workflow confirms the detection method is correctly interpreting methylated cytosines, helping catch conversion efficiency problems before they affect interpretation of real sample results.
Use methylated dNTP-synthesized DNA to study methylation-sensitive restriction digestion
Some restriction enzymes specifically cleave methylated sequences while others are inhibited by methylation, and a fully methylated DNA produced with 5-methyl-dCTP is a useful substrate for confirming whether a specific enzyme's activity is methylation-sensitive.
Be aware that 5-methyl-dCTP is also believed to be the starting substrate for TET enzyme oxidation
5-methylcytosine is believed to be the substrate for TET family enzymes that produce 5-hydroxymethylcytosine and further oxidation products, making 5-methyl-dCTP-synthesized DNA a potential substrate for in vitro TET enzyme activity studies.
Confirm nucleotide concentration and compatibility with your synthesis conditions
Standard dNTPs and modified analogs like 5-methyl-dCTP are typically supplied as concentrated solutions requiring dilution, and confirming appropriate concentration and buffer compatibility before use ensures consistent, reproducible incorporation into your target DNA.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
5-methyl-dCTP is typically supplied as a 10 mM solution and offers the ability to enzymatically make a defined, fully methylated, cytosine-substituted DNA by PCR or in vitro synthesis, a property that makes it a valuable resource for a variety of biochemical and cellular applications in epigenetics research. As of 2026, methylated DNA probes made with 5-methyl-dCTP continue to serve as methylation reference fragments and as pull-down substrates for 5-hydroxymethylcytosine-binding proteins from cellular lysates, extending their use beyond simple positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find high-integrity dNTPs tailor-made for your research applications.

dNTPs (Deoxynucleotide Triphosphates)

dATP (100 mM) (250 µl)

D1005 by Molecular Biology Products (MBP Inc.) is a 100 mM dATP (deoxyadenosine triphosphate) solution supplied in a 250 µL volume. Ready for direct use in PCR, RT-PCR, nucleotide mix preparation, library preparation, and other molecular biology applications requiring individual dNTP components at a defined molar concentration.

Concentration

dCTP (100 mM) (250 µl)

D1020 by Zymo Research is 100 mM dCTP solution (250 µl, 25 µmol) with >99% trisphosphate purity by HPLC, free of nuclease and phosphatase contaminants. Use 0.5 µl per 50 µl PCR reaction for a 0.25 mM final concentration; compatible with ZymoTaq™ and other standard DNA polymerases. Store at -20°C (12-month guarantee) or -80°C for extended periods. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

dGTP (100 mM) (250 µl)

C2011-2 by Zymo Research — 2-pack of Air Permeable Sealing Covers for 96-well microcentrifuge spin-column and collection plates. Allows gas exchange while preventing cross-contamination and reducing evaporation during incubation steps. Compatible with Zymo-Spin I-96-XL Plates (C2010). Also available in 4-pack (C2011-4) and 8-pack (C2011-8).

dTTP (100 mM) (250 µl)

D1010 by MBP is a 100 mM dTTP (deoxythymidine triphosphate) solution supplied in a 250 µl volume, formulated for direct use in PCR, RT-PCR, nucleotide mix preparation, and library construction protocols. Store at -20°C.

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

dsDNA Shearase

dsDNA Shearase (E2019-200) by Zymo Research — dsDNA Shearase Plus endonuclease (1 U/µL, 200 U) bundled with DNA Clean &amp; Concentrator-5 (DCC-5). Fragments 250 ng human DNA to 100–500 bp at 42°C in 20 min; elute purified fragments in as little as 10 µL. Ready for NGS and MeDIP workflows.

Enzyme Inactivation

Unit Definition

dsDNA Shearase™ (200 U)

dsDNA Shearase™ Plus (50 U)

dsDNA Shearase Plus

dsDNA Shearase Plus (E2018-200) by Zymo Research — 1 U/µL, 200 U. Fragments 250 ng human DNA to 100–500 bp in 20 min at 42°C in 10 µL. Generates 5′-phosphate/3′-OH termini ready for NGS adapter ligation. Enzyme only; for the DCC-5 bundle see E2019-200.

dsDNA Shearase™ Plus (200 U)

<h2>What are methylated dNTPs?</h2>
&nbsp;
Methylated dNTPs are base-modified deoxynucleotide analogs, the most important being 5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate), in which the cytosine base carries a methyl group at the carbon-5 position. This is the same position methylated in naturally occurring genomic 5-methylcytosine, the primary DNA methylation mark studied in epigenetics research. Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction or in vitro synthesis produces a defined, fully cytosine-methylated DNA template from a known sequence.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
5-Methyl dCTP (10 mM) (100 &micro;l): for high-purity enzymatic incorporation into methylated DNA strands.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
5-Methylcytosine dNTP Mix (10 mM) (250 &micro;l): pre-validated nucleotide blends for controlled epigenetic assay workflows.
</li>
</ul>
&nbsp;
<h2>How to use methylated dNTPs</h2>
&nbsp;
Substitute 5-methyl-dCTP for dCTP in PCR to synthesize defined methylated DNA
A PCR reaction using 5-methyl-dCTP in place of standard dCTP produces a fully methylated amplicon in which every cytosine carries the 5-methyl modification, providing a positive control with a known and consistent methylation status distinct from the partial, variable methylation of a genomic DNA control.
Use methylated DNA as a positive control for bisulfite or enzymatic conversion workflows
Running 5-methyl-dCTP-synthesized fully methylated DNA through your bisulfite or enzymatic conversion protocol confirms the method correctly preserves methylated cytosines while converting unmethylated ones, catching conversion efficiency problems at the control stage before they affect interpretation of real samples.
Test methylation-sensitive restriction enzymes using 5-methyl-dCTP-produced substrates
Some restriction enzymes cut methylated sequences while others are inhibited by methylation at their recognition site, and a PCR-synthesized, fully methylated substrate of known sequence provides a clean, defined test of whether a specific enzyme is methylation-sensitive or methylation-dependent.
Use as an in vitro substrate for TET enzyme oxidation studies
Since 5-methylcytosine is the starting substrate for TET family enzyme oxidation into 5-hydroxymethylcytosine and further products, fully methylated DNA synthesized with 5-methyl-dCTP provides a defined, homogeneous substrate for in vitro TET enzyme activity assays.
Store methylated dNTPs appropriately to maintain activity
Methylated dNTP analogs are generally stored at -20&deg;C, similar to standard dNTPs, with short-term exposure to ambient temperature acceptable but prolonged room-temperature storage avoided to prevent hydrolysis of the phosphate groups.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
5-methyl-dCTP is typically supplied as a 10 mM solution at pH 7.0 and can be diluted using sterile distilled water or TE buffer, and it can be used in PCR, RT-PCR, and other DNA synthesis applications at the same concentration as standard dCTP in a reaction mix, with 5-methylated DNA probes made this way serving as methylation reference fragments or as pull-down substrates for 5-hydroxymethylcytosine-binding proteins. As of 2026, 5-methyl-dCTP-synthesized control DNA remains a standard tool in epigenetics research labs specifically because it provides a modification-defined substrate without relying on the variable or partially characterized methylation patterns of genomic positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and get high-quality methylated (dNTPs) for your lab.

Methylated

5-Methylcytosine dNTP Mix (10 mM) (250 µl)

dNTP mixture containing the modified deoxynucleotide 5-mC for use with PCR, mass spec, etc.