DNA/RNA Methylation

<h2>What are bisulfite conversion kits?</h2>
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Bisulfite conversion kits are all-in-one systems that bundle every reagent and column needed for the sodium bisulfite conversion workflow into a single, pre-optimized format, covering the denaturation and sulfonation steps, desulfonation with an alkali reagent, and a post-conversion cleanup step that removes the bisulfite and other reaction byproducts before the converted DNA is used in downstream analysis. This contrasts with standalone Bisulfite Conversion Reagents &amp; Buffers, which lets labs assemble their own optimized protocol from individual components.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Standard bisulfite conversion kits for routine DNA methylation analysis&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Combined transcriptomic and epigenetic studies with RNA and DNA methylation conversion kits</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well bisulfite conversion kits for wider-scale screening applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Automation-compatible bisulfite kits for simplified workflow integration</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Magnetic bead-based bisulfite conversion kits for enhanced purification and recovery efficiency</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Direct bisulfite conversion kits for streamlined sample processing&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Rapid conversion kits for methylation analysis workflows that are time-sensitive&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Starter kits for validation and optimized setup of bisulfite conversion protocols</li>
</ul>
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<h2>How to choose a bisulfite conversion kit</h2>
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Match kit format to your downstream sequencing or PCR application
Kit versions optimized for WGBS work at larger input scales with library prep in mind, while RRBS-compatible kits often include restriction enzyme digestion components to enrich CpG-dense regions before conversion, and MSP-focused kits may include primers or protocol guidance for post-conversion methylation-specific PCR.
Check the kit's validated input DNA range against your sample type
Different bisulfite conversion kits are validated for different input DNA ranges, and kits validated for low-input or cell-free DNA samples specifically address the additional fragmentation and yield challenges that these sample types face during the harsh chemical conversion step.
Confirm the kit includes an unmethylated conversion control or specify one separately
A quality bisulfite conversion kit should provide, or clearly specify, an unmethylated control to run alongside samples, since tracking conversion efficiency across the kit's components helps catch kit-specific variability in conversion completeness between different lots or runs.
Consider kits with optimized FFPE protocols when working with formalin-fixed tissue
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before conversion; kits with FFPE-specific protocol variants include modifications like shortened incubation times and carrier reagents to minimize the further fragmentation this already-compromised input type would suffer under standard conversion conditions.
Evaluate throughput format options to match your sample volume
Some bisulfite conversion kits are available in spin column formats suited to individual sample processing, while plate-based or automation-compatible formats support higher-throughput sample sets, making format an important practical consideration beyond kit chemistry alone.
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<h2>Specifications context</h2>
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Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis at single-base resolution, and a key advantage of using a complete kit over standalone reagents is that the included cleanup step reliably removes residual bisulfite and salt from the converted DNA, which would otherwise inhibit downstream PCR amplification and library preparation. As of 2026, enzymatic conversion kits (covered in Non-Bisulfite Kits &amp; Reagents) are increasingly positioned as an alternative that avoids the DNA damage introduced by chemical bisulfite treatment, though bisulfite conversion kits remain broadly used and well-validated across the largest range of downstream applications.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get high-quality bisulfite conversion kits for your lab.

Bisulfite Conversion Kits

Data Sheet

Protocol

EZ DNA Methylation-Direct™ Kit

The EZ DNA Methylation-Direct™ Kit (D5020) by Zymo Research performs &gt;99.5% bisulfite conversion directly from blood, tissue, cells, FFPE, and LCM samples — no prior DNA purification required. Accepts 50 pg–2 µg DNA or 10–10⁵ cells; elutes in ≥10 µl. Compatible with PCR, sequencing, microarrays, and NGS. 50 reactions (D5020) or 200 reactions (D5021).

Applications

Conversion

Elution Volume

Equipment

Input

Processing Time

Recovery

Sample Source

Manufacturer

Shipping Condition

Storage Condition

EZ DNA Methylation-Direct™ Kit (50 Rxns.)

EZ DNA Methylation-Direct™ Kit (200 Rxns.)

D5005-MSDS

D5005-Protocol

EZ DNA Methylation-Gold™ Kit

D5005 by Zymo Research converts unmethylated cytosines to uracil with &gt;99% efficiency in under 3 hours at 64°C, using 500 pg–2 µg of input DNA. The EZ DNA Methylation-Gold™ Kit (50 rxns) supports bisulfite sequencing, MSP, pyrosequencing, Illumina EPIC/450K arrays, and endonuclease-based methylation analysis. All reagents store at room temperature; converted DNA is stable at −20°C.

EZ DNA Methylation-Gold™ Kit (50 Rxns.)

EZ DNA Methylation-Gold™ Kit (200 Rxns.)

DataSheet

MSDS

Recommendations

EZ DNA Methylation-Lightning Automation Kit (1 x 96 prep)

The D5049 by Zymo Research is a 96-prep EZ DNA Methylation-Lightning Automation Kit combining &gt;99.5% bisulfite conversion efficiency (98°C 8 min + 54°C 60 min) with automated magnetic bead purification compatible with Hamilton STAR/STARlet, Tecan Fluent, and KingFisher Flex robotics. Input range 100 pg–2 µg; &gt;80% recovery; A260/A280 ≥ 1.8; optimized for Illumina Infinium arrays, WGBS, and MSP workflows.

Binding Capacity

Conversion Efficiency

DNA Purity

Input Recovery

Required Equipment

User Provided Materials

Data Sheet (Fragmented DNA)

Data Sheet (NGS)

D5030/D5031-MSDS

D5030E/D5031E-MSDS

D5030/D5031-Protocol

D5030E/D5031E-Protocol

EZ DNA Methylation-Lightning Kit

D5030 EZ DNA Methylation-Lightning™ Kit by Zymo Research delivers >99.5% bisulfite conversion efficiency with >80% DNA recovery from 100 pg–2 µg input in under 1.5 hours, 50 reactions, all components stable at room temperature.

EZ DNA Methylation-Lightning™ Kit (50 Rxns.)

EZ DNA Methylation-Lightning™ Kit (50 Rxns.) (CE-IVD)

EZ DNA Methylation-Lightning™ Kit (10 Rxns.)

EZ DNA Methylation-Lightning™ Kit (200 Rxns.)

EZ DNA Methylation-Lightning™ Kit (200 Rxns.) CE-IVD

EZ DNA Methylation-Startup™ Kit (50 Rxns.)

D5024 by Zymo Research is the EZ DNA Methylation-Startup™ Kit (50 rxns) — an all-inclusive bisulfite conversion kit including EZ DNA Methylation-Direct Kit, Universal Methylated Human DNA Standard with control primers, and ZymoTaq DNA Polymerase PreMix. Conversion efficiency >99.5%; input 50 pg–2 µg; recovery >80%; 4-hour processing.

Applicable For

D5001-MSDS

D5001-Protocol

D5001-App Note

EZ DNA Methylation™ Kit

D5001 by Zymo Research is the EZ DNA Methylation Kit — a 50-reaction bisulfite conversion system delivering &gt;99% cytosine conversion from 500 pg to 2 µg input DNA, with &gt;80% recovery and ≥10 µl elution volume. Validated for Illumina Infinium MethylationEPIC BeadChip, NGS bisulfite sequencing, MSP, and pyrosequencing. Requires a thermocycler with heated lid; total processing time 12–16 hours. Made in USA by Zymo Research.

EZ DNA Methylation™ Kit (50 Rxns.)

EZ DNA Methylation™ Kit (200 Rxns.)

EZ RNA Methylation™ Kit

EZ RNA Methylation™ Kit R5001 by Zymo Research combines RNA denaturation and bisulfite conversion in a single step, achieving &gt;99% cytosine conversion and &gt;80% RNA recovery from 0.5–1 µg input in 50 minutes. Converted RNA is compatible with RT-PCR, Sanger sequencing, next-generation sequencing library preparation, and microarray analysis for RNA m5C methylation profiling.

Purity

EZ RNA Methylation™ Kit (50 Preps.)

EZ RNA Methylation™ Kit (200 Preps.)

D5023-Protocol

EZ-96 DNA Methylation-Direct Kit

EZ-96 DNA Methylation-Direct Kit (D5022) by Zymo Research — 96-well shallow format, 192 total reactions. Direct bisulfite conversion from blood, tissue, cells, or FFPE without prior DNA extraction. Input: 10 cells to 10⁵ cells or 50 pg–2 µg DNA. Elutes in 30 µL. WGBS and targeted methylation ready.

Deep-well

EZ-96 DNA Methylation-Direct™ Kit (Shallow-well) (2 x 96 Rxns.)

EZ-96 DNA Methylation-Direct™ Kit (Deep-well) (2 x 96 Rxns.)

App Note with Tecan

EZ-96 DNA Methylation-Direct MagPrep

D5044 by Zymo Research — EZ-96 DNA Methylation-Direct MagPrep; 96-well magnetic bead bisulfite conversion; &gt;99.5% conversion; 50 pg–2 µg input; direct from blood, tissue, FFPE, LCM; ≥25 µL elution; ~4 hr; 4×96 rxns.

EZ-96 DNA Methylation-Direct™ MagPrep (4 x 96 Rxns.)

EZ-96 DNA Methylation-Direct™ MagPrep (8 x 96 Rxns.)

EZ-96 DNA Methylation-Gold MagPrep

D5042 by Zymo Research converts 96 DNA samples per plate to bisulfite form with &gt;99% efficiency using a two-stage protocol (98°C/10 min + 64°C/2.5 hrs) and magnetic bead cleanup. The EZ-96 DNA Methylation-Gold™ MagPrep (4×96 reactions) is validated for the Tecan Freedom EVO and compatible with Illumina EPIC arrays and WGBS. D5043 provides 8×96 reactions.

EZ-96 DNA Methylation-Gold™ MagPrep (4 x 96 Rxns.)

EZ-96 DNA Methylation-Gold™ MagPrep (8 x 96 Rxns.)

D5007-Protocol

D5008-Protocol

EZ-96 DNA Methylation-Gold™ Kit

D5007 by Zymo Research is a 2 × 96 reaction EZ-96 DNA Methylation-Gold Kit using one-step thermal denaturation + bisulfite conversion at 64°C for 2.5 hours—enabling same-day processing. Achieves &gt;99% conversion efficiency, &gt;75% DNA recovery, 30 µl elution. Accepts 500 pg–2 µg input DNA per well. Compatible with WGBS, RRBS, MSP, pyrosequencing, and Illumina methylation arrays.

EZ-96 DNA Methylation-Gold™ Kit (2 x 96 Rxns.) - Shallow Well

EZ-96 DNA Methylation Gold™ Kit (2 x 96 Rxns.) - Deep Well

D5046/D5047-MSDS

D5046-E/D5047-E-MSDS

D5046/D5047-Protocol

D5046-E/D5047-E-Protocol

App Note with Hamilton

EZ-96 DNA Methylation-Lightning MagPrep

D5046 by Zymo Research processes 96 samples per plate via magnetic beads, achieving &gt;99.5% bisulfite conversion in ~68 minutes with 100 pg–2 µg DNA input. The EZ-96 DNA Methylation-Lightning™ MagPrep (4×96 reactions) is validated for the Tecan Freedom EVO and compatible with Illumina EPIC arrays, WGBS, and targeted BS-Seq. D5047 provides 8×96 reactions for larger cohorts.