DNA/RNA Methylation

<h2>What are non-bisulfite methylation kits and reagents?</h2>
&nbsp;
Non-bisulfite methylation kits and reagents are alternatives to sodium bisulfite conversion that achieve the same goal &mdash; distinguishing methylated from unmethylated cytosines at single-base or regional resolution &mdash; without the harsh chemical treatment that bisulfite introduces. The most widely adopted approach is enzymatic methyl-seq (EM-seq), which uses TET2 to oxidize 5-methylcytosine and 5-hydroxymethylcytosine and T4-BGT to protect them from subsequent APOBEC3A-mediated deamination, so that only unmodified cytosines are converted to uracil.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">qMethyl PCR assay kit uses PCR amplification for quantitative DNA methylation detection</li>
<li dir="ltr" role="presentation">One-step qMethyl PCR system for efficient methylation analysis and optimized workflow setup&nbsp;</li>
<li dir="ltr" role="presentation">Test reaction premixes for validation and optimization of assays&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose non-bisulfite methylation reagents</h2>
&nbsp;
Choose EM-seq for superior library quality over bisulfite at the same resolution
EM-seq outperforms bisulfite-converted libraries on all major quality metrics: higher library yield, fewer duplicates, more usable reads, more even GC distribution, and accurate methylation calling, without requiring any changes to downstream bioinformatic analysis pipelines since the output is the same C-to-T chemistry as bisulfite conversion.
Understand EM-seq's two-step enzymatic mechanism
In the first EM-seq step, TET2 oxidizes 5-mC and 5-hmC to protected forms while T4-BGT glucosylates 5-hmC; in the second step, APOBEC3A deaminates only the unprotected unmodified cytosines to uracil. The net result is the same as bisulfite conversion &mdash; unmethylated C reads as T &mdash; but without bisulfite's destructive chemistry.
Consider TAPS for mild, nondestructive conversion that directly marks modified cytosines
TET-assisted pyridine borane sequencing (TAPS) is a bisulfite-free method that combines TET oxidation of 5-mC and 5-hmC with pyridine borane reduction, converting modified cytosines to a thymine read while leaving unmodified cytosines intact. Unlike bisulfite and EM-seq, TAPS positively marks the modified bases rather than converting the unmodified ones, and can preserve DNA fragments over 10 kilobases.
Choose MeDIP for genome-wide enrichment without per-cytosine resolution
Methylated DNA immunoprecipitation uses an antibody specific to 5-methylcytosine or 5-hydroxymethylcytosine to pull down methylated DNA fragments, providing regional enrichment data at lower cost and without bisulfite-related DNA damage, though at lower resolution than sequencing-based conversion methods.
Confirm whether your method distinguishes 5-mC from 5-hmC or co-detects both
EM-seq detects both 5-mC and 5-hmC together and cannot distinguish them without additional steps, while TAPS also co-detects both; TAPS&beta;, a variant, can specifically call 5-mC by blocking 5-hmC before oxidation. MeDIP can be targeted specifically to 5-hmC (hMeDIP) using an anti-5-hmC antibody.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
EM-seq was effective using as little as 100 pg of DNA input, and libraries showed higher yields with fewer PCR cycles than bisulfite-converted libraries at all input amounts tested, with more even GC distribution, reduced sequencing duplicates, and more CpGs detected within genomic features per sequencing read. As of 2026, enzymatic conversion via EM-seq is broadly recommended over bisulfite conversion for new methylome analysis projects, particularly those working with limited, degraded, or high-value samples where the DNA damage of bisulfite treatment is most consequential.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, and is closely related to DNA Hydroxymethylation for 5-hmC-specific approaches. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at Molecular Biology Products Inc. (MBP)">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find dependable non-bisulfite kits and reagents for your lab.

Non-Bisulfite Kits & Reagents

MSDS

2X Test Reaction PreMix

2X Test Reaction PreMix (D5310) by Zymo Research — 500 µl, contains MSREs and SYTO 9 dye for bisulfite-free locus-specific DNA methylation quantification in the OneStep qMethyl Kit. Compatible with Bio-Rad CFX, QuantStudio, and LightCycler 480. Store at −20°C. Made in the USA.

Manufacturer

Shipping Condition

Storage Condition

2X Reference Reaction PreMix

2X Test Reaction-Lite PreMix

The 2X Test Reaction-Lite PreMix (D5311) by Zymo Research is the dye-free bisulfite-free DNA methylation quantitation component for the OneStep qMethyl-Lite system. Uses methylation-sensitive restriction enzymes (MSREs) to calculate percent methylation from qPCR ΔCt values — no bisulfite conversion required. Researcher supplies compatible fluorescent dye or TaqMan probe; input 5 ng–1 µg DNA; amplicons &gt;150 bp.

2X Reference Reaction-Lite PreMix

<h2>What are ELISA kits for methylation in this category?</h2>
&nbsp;
ELISA kits for DNA and RNA methylation in this category are antibody-based assays that quantify the global level of a specific epigenetic mark, most commonly 5-methylcytosine in genomic DNA, 5-hydroxymethylcytosine in genomic DNA, or N6-methyladenosine in RNA, using a plate-based immunoassay format without requiring bisulfite conversion or sequencing. Denatured, single-stranded DNA or RNA from a sample is coated on a plate well, and a mark-specific primary antibody followed by an HRP-conjugated secondary antibody generates a colorimetric signal proportional to the level of the target modification.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">5-mC DNA ELISA kits for quantitative analysis of DNA methylation&nbsp;</li>
<li dir="ltr" role="presentation">DNA controls for validation and calibration of the assay to ensure experimental&nbsp; reproducibility and accuracy&nbsp;</li>
<li dir="ltr" role="presentation">Secondary antibody reagents for specific signal generation and detection in ELISA assays</li>
<li dir="ltr" role="presentation">Coating buffers for consistent assay performance and optimized plate preparation&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose methylation ELISA kits and reagents</h2>
&nbsp;
Match your kit to the specific epigenetic mark you're quantifying
Different kits use antibodies against specific modifications: 5-methylcytosine ELISA kits quantify global DNA methylation, 5-hmC ELISA kits quantify global hydroxymethylation, and m6A ELISA kits quantify RNA N6-methyladenosine. Using a kit with the wrong antibody specificity will not report the mark you intend to measure.
Use methylation standards to build a calibration curve for quantitative results
Running a series of methylation standards with defined percentages alongside your samples allows the absorbance readout to be converted to a specific, meaningful methylation percentage, since the raw ELISA signal alone cannot be interpreted as absolute methylation level without a calibration.
Understand that ELISA measures global levels, not locus-specific methylation
A methylation ELISA gives the overall average percentage of methylation across the genome in a sample, which is useful for comparing broad methylation changes between conditions but cannot identify which specific genes or regions are differentially methylated the way bisulfite sequencing can.
Confirm the required DNA denaturation step is appropriate for your sample type
ELISA kits for DNA methylation typically require single-stranded, denatured DNA coated directly on the plate, and confirming this denaturation step is properly performed is necessary for consistent antibody binding and reproducible signal across wells.
Be aware of cross-reactivity with similar modifications when interpreting results
An antibody against 5-methylcytosine may have some cross-reactivity with 5-hydroxymethylcytosine, and confirming the antibody's published cross-reactivity data helps interpret whether your signal specifically reflects 5-mC, 5-hmC, or both, which matters for studies in cell types where 5-hmC is abundant.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The 5-methylcytosine ELISA workflow uses an indirect ELISA methodology where denatured, single-stranded DNA samples are coated directly on the plate well surfaces and a 5-mC monoclonal antibody and an HRP-conjugated secondary antibody are added sequentially, generating a colorimetric signal proportional to the total level of methylated cytosine in the sample. As of 2026, methylation ELISA kits are particularly valued for high-throughput screening studies comparing global methylation levels across many treatment conditions or patient groups where single-base-resolution data is not the primary goal.
This category sits within DNA/RNA Methylation, alongside Methylation Standards for calibration, and is related to DNA Hydroxymethylation and Non-Bisulfite Kits &amp; Reagents for higher-resolution methylation analysis. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of&nbsp; Molecular Biology Products Inc. (MBP)</a> to get high-quality ELISA Kits and Reagents for your lab.

ELISA Kits & Reagents

DataSheet

D5325-1-15/D5325-1-30 - MSDS

5-mC Coating Buffer

D5325-1-15 5-mC Coating Buffer by Zymo Research — 15 ml, stored at 4°C — coating medium for denatured ssDNA in 5-methylcytosine quantification ELISA assays. Compatible with 5-mC DNA ELISA Kit (D5325, D5326).

5-mC Coating Buffer (15 ml)

5-mC Coating Buffer (30 ml)

5-mC ELISA Buffer (250 ml)

Protocol

5-mC DNA ELISA Kit (2 x 96 Preps.)

D5326 by Zymo Research is a 5-mC DNA ELISA Kit for colorimetric quantification of global 5-methylcytosine in purified DNA. Accepts 10–200 ng DNA per well, detects methylation above 0.5% 5-mC, and completes in under 3 hours. Kit contains reagents for 2 × 96 reactions.

Detection

Equipment

Input

Sample Source

5-mC DNA ELISA Kit (96 Preps.)

The 5-mC DNA ELISA Kit (D5325) by Zymo Research quantifies global 5-methylcytosine content in 96 samples using a colorimetric anti-5-mC antibody assay (Clone 7D21) with ≥0.5% detection sensitivity, a 7-point standard curve, and a total assay time under 3 hours.

<h2>What is DNA/RNA Methylation as a reagent category?</h2>
&nbsp;
DNA/RNA Methylation covers the detection and quantification side of epigenetics: the reagents used to identify which cytosines in a DNA sample are methylated, distinguish 5-methylcytosine from 5-hydroxymethylcytosine, and detect RNA methylation marks like N6-methyladenosine (m6A). Methods range from bisulfite conversion, the historically standard approach for single-base-resolution cytosine methylation mapping, to enzymatic conversion as a less damaging alternative, to antibody-based immunoprecipitation for broad-scale enrichment of methylated DNA regions, to direct ELISA-based quantification of global methylation levels.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/methylation-standards">DNA methylation standards:</a> for accurate quantitative analysis and calibration of methylation levels
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-kits">Bisulfite conversion kits: </a>for efficient conversion of unmethylated cytosines in DNA methylation studies
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-reagents-and-buffers">Bisulfite Conversion Reagents and Buffers: </a>for enhanced processing of DNA and flexible workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/non-bisulfite-kits-and-reagents">Non-Bisulfite Kits and Reagents: </a>for alternative epigenetic profiling and methylation analysis methods
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/elisa-kits-and-reagents" style="color: #236fa1;">ELISA Kits and Reagents:</a> for quantifying sensitive DNA/RNA methylation levels
</li>
</ul>
&nbsp;
<h2>How to choose DNA/RNA methylation analysis reagents</h2>
&nbsp;
Choose bisulfite conversion for single-base-resolution cytosine methylation mapping
Bisulfite conversion converts unmethylated cytosines to uracil while leaving 5-methylcytosine largely unchanged under standard conditions, and after amplification and sequencing, the resulting base-pair difference reveals exactly which cytosines were methylated, making it the approach for single-base-resolution mapping but one that introduces significant DNA damage and degradation.
Consider enzymatic conversion as a less damaging alternative to bisulfite treatment
Enzymatic conversion methods achieve the same cytosine-to-uracil discrimination as bisulfite but through enzymatic rather than harsh chemical reactions, minimizing DNA damage and producing higher-quality libraries from fewer sequencing reads, at potentially higher reagent cost.
Use antibody-based MeDIP for broad methylation enrichment without locus-by-locus resolution
Methylated DNA immunoprecipitation uses an antibody against 5-methylcytosine or 5-hydroxymethylcytosine to enrich methylated DNA regions from a fragmented sample, providing genome-wide enrichment data without the single-base resolution of bisulfite sequencing.
Confirm your method can distinguish 5-mC from 5-hmC if that difference matters for your study
Standard bisulfite conversion cannot reliably distinguish 5-methylcytosine from 5-hydroxymethylcytosine, since both resist bisulfite-mediated deamination under standard conditions, so confirming whether your study requires that distinction helps determine whether a specialized additional step is needed.
Choose ELISA-based kits for rapid, global methylation quantification without sequencing
A 5-methylcytosine ELISA kit provides a rapid, quantitative readout of the global level of DNA methylation in a sample without requiring bisulfite conversion or sequencing, useful for broad-level comparisons across multiple conditions or samples when single-base resolution isn't needed.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its single-base resolution, but it introduces significant technical challenges for amplification and next-generation sequencing library preparation due to DNA degradation, reduced genome complexity, and alignment complexity from the cytosine-to-uracil base conversion. As of 2026, enzymatic conversion is increasingly recommended over bisulfite conversion for methylation analysis specifically because it minimizes these DNA damage and quality issues, enabling improved preservation and detection of cytosine methylation marks from fewer sequencing reads with no change to downstream analysis pipelines.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> today and get reliable DNA/RNA methylation kits for your lab.

Anti-5-Hydroxymethylcytosine Polyclonal Antibody

A4001-ZYM by Zymo Research — Anti-5-Hydroxymethylcytosine Polyclonal Antibody, rabbit IgG, 1 mg/ml in PBS with 0.05% sodium azide. Validated for hmeDIP, ELISA, immunohistochemistry, and dot blot detection of 5-hmC in DNA from mammalian, plant, insect, and microbial sources. Available in 25, 50, and 200 µg sizes.

Buffer Solution

Isotype

Protein Concentration

Source

Specificity

Storage

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (200 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (25 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (50 µg)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl)

A3002-ZYM by Zymo Research — Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21), mouse IgG1, 0.5 µg/µl in PBS with 0.05% sodium azide. Validated for MeDIP, ELISA, and dot blot; specific for 5-mC in single-stranded DNA with no detectable cross-reactivity to 5-hmC or unmodified cytosine. Available in 15–200 µl sizes.

Clone

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (15 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (200 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (30 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (50 µl)

<h2>What are methylation standards?</h2>
&nbsp;
Methylation standards are defined reference DNA materials with a known and confirmed methylation status, ranging from a fully methylated DNA (where every cytosine at CpG or other sites carries a methyl group) to a fully unmethylated DNA (where no cytosines are methylated) and intermediate or mixed standards containing a defined ratio of the two. The most widely used unmethylated control in bisulfite sequencing is lambda phage DNA, which is naturally unmethylated and can be spiked into a conversion reaction to estimate conversion efficiency.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Methylated and non-methylated DNA control sets for comparative epigenetic analysis and assay validation</li>
<li dir="ltr" role="presentation">Universal methylated DNA standards for methylation assessment workflows</li>
<li dir="ltr" role="presentation">Genomic DNA reference materials: for microbial and human methylation studies</li>
<li dir="ltr" role="presentation">Matched DNA sets: used in experimental models for controlled comparison of methylation status&nbsp;</li>
<li dir="ltr" role="presentation">Bisulfite-converted DNA standards: for downstream sequencing-based methylation and PCR analysis</li>
<li dir="ltr" role="presentation">Methylation spike-in controls: for sequencing normalization and high-throughput workflows</li>
<li dir="ltr" role="presentation">Mouse and human DNA methylation reference sets: for specific species epigenetic studies</li>
<li dir="ltr" role="presentation">Methylated DNA IP control kits for methylation assays involving immunoprecipitation</li>
</ul>
&nbsp;
<h2>How to use methylation standards</h2>
&nbsp;
Spike an unmethylated control into every bisulfite or enzymatic conversion reaction
Running an unmethylated DNA like lambda phage DNA alongside your samples in the same conversion reaction estimates the conversion efficiency of that specific run, since every remaining cytosine signal after conversion should be treated as a false positive, and this rate can be quantified and accounted for in downstream analysis.
Run a fully methylated positive control to confirm methylated cytosines are preserved
A fully methylated standard, such as DNA synthesized with 5-methyl-dCTP or commercially prepared methylated genomic DNA, confirms your conversion workflow correctly preserves methylated cytosines intact rather than inadvertently converting them, which would produce false-negative methylation calls.
Use a mixed or intermediate standard to calibrate quantitative methylation assays
A standard containing a defined ratio of methylated to unmethylated DNA, or a set of standards spanning a range of methylation percentages, provides a calibration curve for quantitative methylation assays like 5-methylcytosine ELISA, validating that the assay reports the expected methylation level across its full dynamic range.
Confirm standard stability before long-term storage or lot-to-lot use
Methylation control DNA has documented stability windows, and confirming that your standard's methylation status is stable under storage conditions, and consistent across lots, is necessary for reliable longitudinal monitoring of conversion efficiency.
Use standards to monitor workflow consistency over time
Running a conversion control in every batch allows longitudinal tracking of conversion efficiency across instrument runs, reagent lots, and protocol variations, helping catch workflow drift before it affects real sample results.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Lambda phage DNA is the most commonly used unmethylated bisulfite conversion control because it is naturally unmethylated, commercially available, and well characterized, and it can be aligned to the known lambda phage reference genome after sequencing to calculate residual unconverted cytosine rates, providing a direct, interpretable measure of conversion efficiency. As of 2026, fully methylated and fully unmethylated standard sets are also used alongside enzymatic methyl-seq conversions to confirm that EM-seq's enzymatic mechanism correctly protects methylated cytosines and converts unmethylated ones, independent of the conversion chemistry used.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, Non-Bisulfite Kits &amp; Reagents, and ELISA Kits &amp; Reagents. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of Molecular Biology Products Inc. (MBP)</a> to get high-quality methylation standards for your lab today.

Methylation Standards

Bisulfite Converted Universal Methylated Human DNA Standard

Bisulfite Converted Universal Methylated Human DNA Standard (D5015) by Zymo Research — pre-converted human brain genomic DNA with >95% CpG methylation, 20 ng/µl, 1 µg/50 µl (50 reactions). Includes MLH1 validation primers.

Concentration

Sample Storage

<h2>What are bisulfite conversion reagents and buffers?</h2>
&nbsp;
Bisulfite conversion reagents include sodium bisulfite solution, denaturation and conversion buffers, and complete kits that bundle all components needed for the three-step chemical reaction: sulfonation of cytosine to a cytosine-bisulfite derivative, hydrolytic deamination to a uracil-bisulfite derivative, and alkali desulfonation to yield uracil. This process converts unmethylated cytosines to uracil while leaving 5-methylcytosine intact and unconverted, because 5-methylcytosine resists bisulfite-mediated deamination under standard conditions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">RNA and DNA conversion reagents for enhanced modification of cytosine in methylation workflows</li>
<li dir="ltr" role="presentation">Desulphonation buffers for stabilization of nucleic acids and post-conversion cleanup&nbsp;</li>
<li dir="ltr" role="presentation">Binding buffers used during purification for efficient capture of converted RNA and DNA</li>
<li dir="ltr" role="presentation">Wash buffers for the removal of inhibitors and improved purity of samples</li>
<li dir="ltr" role="presentation">Elution buffers for high-quality recovery of converted nucleic acids</li>
<li dir="ltr" role="presentation">DNA denaturing and digestion buffers for controlled workflow and optimized conversion efficiency</li>
<li dir="ltr" role="presentation">Dilution and reaction buffers for maintaining stable assay conditions</li>
<li dir="ltr" role="presentation">Solubilization and dissolution buffers for sample processing and preparation&nbsp;</li>
<li dir="ltr" role="presentation">Lightning conversion reagents for efficient and rapid methylation analysis workflows</li>
</ul>
&nbsp;
<h2>How to use bisulfite conversion reagents</h2>
&nbsp;
Ensure complete DNA denaturation before conversion to avoid incomplete conversion
Bisulfite only acts on cytosines in single-stranded DNA, so complete denaturation of the input DNA is the most critical first step, since failure to fully denature can leave double-stranded regions in which cytosines are shielded from the bisulfite reagent and remain unconverted.
Understand that incomplete conversion produces false-positive methylation results
If the conversion is incomplete, unconverted unmethylated cytosines will read as cytosine after sequencing, which will be incorrectly interpreted as methylated cytosine, artificially inflating the apparent methylation level of the sample in a false-positive direction.
Optimize conversion conditions to balance completeness against DNA degradation
The harsh conditions needed for complete bisulfite conversion, including high bisulfite concentration, elevated temperature, and long incubation, also fragment and degrade DNA, and optimizing conditions that achieve high conversion while minimizing degradation is a key technical challenge in bisulfite workflows.
Include an unmethylated conversion control to estimate residual incomplete conversion
Including an unmethylated control DNA, such as lambda phage DNA, in the same conversion reaction provides a readout of how completely unmethylated cytosines were converted, helping quantify residual incomplete conversion rates and account for them in downstream analysis.
Be aware of specific challenges for FFPE and degraded DNA samples
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before bisulfite treatment, and the additional fragmentation during conversion can result in poor library yields; specific modified protocols and carrier reagents can help reduce further degradation during the conversion step.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its high resolution and accuracy at single-base level, but it also introduces specific DNA quality challenges for downstream library preparation and sequencing, particularly for samples already of poor quality like FFPE tissue. As of 2026, enzymatic conversion is increasingly recommended as the preferred alternative to bisulfite conversion specifically because it achieves comparable discrimination between methylated and unmethylated cytosines while minimizing the DNA damage and library complexity loss that harsh bisulfite chemistry introduces.
This category sits within DNA/RNA Methylation under Epigenetics, and for a synthetic, fully methylated positive control to run through the conversion workflow, see dNTPs (Deoxynucleotide Triphosphates). See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and request a quote today to get high-quality bisulfite conversion reagents for your lab.

Bisulfite Conversion Reagents & Buffers

D5001-1 - MSDS

D5003-1 - MSDS

CT Conversion Reagent

D5001-1 CT Conversion Reagent by Zymo Research — 10 bisulfite conversions; &gt;99% non-methylated C conversion; &gt;99% methylated C protection; input 500&nbsp;pg&ndash;2&nbsp;&micro;g; compatible with EZ DNA Methylation kit series.

CT Conversion Reagent (1 Tube for 10 Conversions)

CT Conversion Reagent (5 x 10 Rxns.)

CT Conversion Reagent (96 Conversions) (1 Bottle)

D5325-5-1-MSDS

D5325-5-2-MSDS

DNA Controls

DNA Controls D5325-5-1 by Zymo Research — Negative Control (0% 5-mC) for the 5-mC DNA ELISA Kit. E. coli genomic dsDNA at 100 ng/µl, 50 µl per unit, stored at −20°C. Used for standard curve construction and assay baseline in global DNA methylation quantification.

Negative Control (100 ng/µl) (50 µl)

Positive Control (100 ng/µl) (50 µl)

DNA Denaturing Buffer (1 ml)

DNA Denaturing Buffer (D5101-4-1) by Zymo Research — 1 ml buffer for DNA strand denaturation in the Methylated-DNA IP Kit (D5101) MeDIP workflow. Stable from 0°C to room temperature. Sold separately or as part of the D5101 kit. Made in the USA.

E. coli Non-Methylated Genomic DNA (5 µg/20 µl)

D5016 by Zymo Research is 5 µg (250 ng/µL, 20 µL) of fully unmethylated E. coli genomic DNA from Dam–/Dcm– strain ER2925, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Used as a negative control or bisulfite conversion efficiency spike-in in WGBS, RRBS, MSP, and epigenetic array workflows. Store at ≤–20°C.