Viral DNA/RNA

<h2>What are 96-Well Plate Viral DNA/RNA Co-Extraction Kits?</h2>
<p>&nbsp;</p>
<p>96-well plate viral DNA/RNA co-extraction kits purify both viral DNA and RNA from up to 96 cell-free samples simultaneously - plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media -- using the same single-buffer lysis chemistry as spin-column viral kits but operating in a Zymo-Spin I-96 spin plate processed with a 96-well plate centrifuge or vacuum manifold. Both analytes co-elute per well in DNase/RNase-free water covering RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, HPV, parvovirus) without requiring robotic automation. The format provides a significant throughput step-up from single-tube spin columns - processing 96 samples in under 30 minutes with a multichannel pipette and standard centrifuge. Choose the 96-well plate format when daily viral sample volumes exceed 24 per run, when automation infrastructure is unavailable, or when batch surveillance testing requires rapid plate-scale turnaround from clinical specimens.</p>
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<h2 data-start="0" data-end="23">What you will find:</h2>
<p data-start="0" data-end="23">&nbsp;</p>
<ul>
<li>Quick-DNA/RNA Viral 96 Kit (D7022)&nbsp;for concurrent isolation of viral DNA and viral RNA from fluid specimens.</li>
<li>96-well plate format for high-throughput sample processing.</li>
<li>Rapid purification workflow for efficient nucleic acid recovery.</li>
<li>Designed for processing multiple samples simultaneously.</li>
<li>Purified viral nucleic acids suitable for downstream molecular biology applications.</li>
</ul>
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<p>&nbsp;</p>
<h2>How to Choose a 96-Well Plate Viral DNA/RNA Co-Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Centrifuge vs. Vacuum Manifold Operation</strong></p>
<p>A 96-well plate centrifuge rotor (accommodating the Zymo-Spin I-96 Plate dimensions) is the standard equipment requirement. If a compatible plate centrifuge is unavailable, a vacuum manifold that fits 96-well spin plates provides equivalent performance and eliminates centrifuge dependency. Confirm equipment compatibility before kit purchase.</p>
<p><strong>Manual vs. Semi-Automated Pipetting</strong></p>
<p>The 96-well format is designed for manual operation with multichannel pipettes for buffer addition and sample loading. Semi-automation is achievable with a liquid handler for buffer dispensing steps, leaving centrifugation as the manual step. For full automation without centrifugation, use the Quick-DNA/RNA Viral MagBead format on a compatible robot.</p>
<p><strong>Lysis Buffer Volume Per Well</strong></p>
<p>The single-buffer lysis system adds 200 ul of lysis buffer per well of sample (1:1 v/v for 200 ul input). Ensure the plate's well volume accommodates the combined lysis buffer + sample volume (400 ul total per well) plus ethanol addition before loading. Most Zymo-Spin I-96 plate protocols include a deep-well collection plate for this mixing step.</p>
<p><strong>Positive and Negative Control Layout</strong></p>
<p>Reserve wells 11 and 12 of each row for extraction controls. Fixed control placement across all plates enables automated data review scripts to flag failed wells based on consistent control positions.</p>
<p><strong>Throughput Scaling Beyond 96</strong></p>
<p>For programs processing 192-384 samples per day, run two 96-well plates sequentially on the same centrifuge or vacuum manifold. For volumes above 384 per day, transition to the magnetic bead format on a liquid handler (KingFisher, Tecan) to process 96 samples per automated run without per-batch manual centrifugation steps.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and DNA/RNA adsorption onto the Zymo-Spin I-96 Plate matrix, with validated recovery of enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A, flaviviruses, measles, parainfluenza, and parvovirus. Nucleic acids are eluted in DNase/RNase-free water suitable for RT-PCR, NGS, and hybridization applications. 96-well plate viral extraction is the standard format in public health and reference laboratory surveillance programs, processing 96-384 clinical specimens per day, including respiratory multiplex panels, arboviral surveillance, and bloodborne pathogen screening, with throughput requirements driving adoption of this format over single-tube spin columns.</p>
<p>&nbsp;</p>
<p>For bulk pricing on Quick-DNA/RNA Viral 96 kits, centrifuge compatibility checks, or guidance on pairing with validated multiplex RT-qPCR assays,&nbsp;<a href="https://mbpinc.net/contact-us"><strong>contact the MBP team</strong></a>.&nbsp;</p>

96-well plate

Protocol

MSDS - D7022

D7023

DataSheet

Quick-DNA/RNA Viral 96 Kit

<p>The Quick-DNA/RNA Viral 96 Kit (D7022) by Zymo Research simultaneously recovers viral DNA and RNA in a 96-well format from plasma, serum, saliva, urine, blood, and other samples — yielding up to 5 µg DNA and 10 µg RNA per prep in DNase/RNase-free water. No organic denaturants or proteases; eluate is directly compatible with RT-PCR, qPCR, and NGS.</p>

Equipment

Purity

Sample Source

Size Range

Manufacturer

Shipping Condition

Storage Condition

Quick-DNA/RNA™ Viral 96 Kit (2 x 96)

Quick-DNA/RNA™ Viral 96 Kit (4 x 96 Preps.)

<h2>What are Magnetic Bead Viral DNA/RNA Co-Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Magnetic bead viral DNA/RNA co-extraction kits purify both viral DNA and RNA from plasma, serum, urine, nasopharyngeal swabs, saliva, blood, cellular suspensions, biopsies, fecal samples, and cell culture media stored in DNA/RNA Shield using paramagnetic silica-coated beads that bind nucleic acids from &gt;50 nt to &gt;200 kb during a single chaotropic lysis step, with magnetic separation during successive wash steps removing inhibitors - no centrifugation required during purification. The 96-well format integrates with KingFisher Flex, Tecan Fluent, and Hamilton STAR liquid-handling platforms, enabling fully automated extraction of both RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, herpesvirus, parvovirus) from 96 samples per run. Choose the magnetic bead format for viral DNA/RNA co-extraction when processing 48+ samples per run, when a compatible automation platform is available, or when the program requires EUA-associated workflows validated for SARS-CoV-2 or other respiratory panel targets.</p>
<p>&nbsp;</p>
<h2 dir="ltr">What you will find:</h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation"><span style="color: #000000;">Magbio HighPrep Viral-Bacterial DNA &amp; RNA Kit (HVB-DR0): Scalable magnetic bead chemistry optimized for the simultaneous extraction of viral and bacterial nucleic acids from diverse fluid profiles.</span></p>
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
<p dir="ltr" role="presentation"><span style="color: #000000;">Magbio HighPrep Viral DNA &amp; RNA Kit (HPV-DR0): High-efficiency magnetic isolation systems engineered for the rapid purification of viral target vectors from fluid specimens.</span></p>
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
<p dir="ltr" role="presentation"><span style="color: #000000;">Magbio HighPrep Viral-Pathogen DNA &amp; RNA Kit (HPP-DR0): Specialized bead technology designed to maximize pathogen recovery and eliminate inhibitors from challenging biological matrices.</span></p>
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
<p dir="ltr" role="presentation"><span style="color: #000000;">Quick-DNA/RNA Viral MagBead (R2140): Advanced magnetic-bead separation configurations built for fast, high-purity co-purification of total viral nucleic acids.</span></p>
</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Magnetic Bead Viral DNA/RNA Co-Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Automation Platform Compatibility</strong></p>
<p>Confirm the kit's automation scripts are available for your specific platform before purchase. The Quick-DNA/RNA Viral MagBead Kit is used in Zymo Research's SARS-CoV-2 EUA workflow and is compatible with KingFisher and other major liquid-handling platforms; contact MBP's technical team or Zymo Research's automation support for current script availability. For Tecan Fluent or Hamilton STAR, platform-specific script development or adaptation may be required.</p>
<p><strong>DNA/RNA Shield as the Collection Medium</strong></p>
<p>The Quick-DNA/RNA Viral MagBead Kit is designed around DNA/RNA Shield as the sample collection, inactivation, and storage medium. Swabs or other samples stabilized in DNA/RNA Shield load directly into the extraction protocol without additional neutralization steps. For programs using VTM (viral transport medium), UTM, or PBS as collection media, confirm compatibility with the kit's lysis buffer by testing spike-in recovery before adopting at scale.</p>
<p><strong>Carrier RNA Requirement</strong></p>
<p>Unlike the QIAGEN QIAamp Viral RNA Mini Kit, the Quick-DNA/RNA Viral MagBead Kit does not require the user to prepare and add carrier RNA to the lysis buffer - the kit's buffer system is formulated for complete lysis and efficient nucleic acid binding without this step. Eliminating the carrier RNA preparation step reduces per-batch setup time and removes one source of inter-batch variability in high-throughput automated workflows.</p>
<p><strong>Nucleic Acid Size Recovery Range</strong></p>
<p>Confirm the kit recovers the size range needed for your downstream application. The Quick-DNA/RNA Viral MagBead Kit binds nucleic acids from &gt;50 nt to &gt;200 kb - covering small viral RNAs (miRNA-sized fragments from viral genomes), standard RT-qPCR amplicon targets, and full-length viral genomes for metagenomic NGS. Applications requiring recovery of very short fragments (&lt;50 nt) need a kit specifically validated for that range.</p>
<p><strong>Regulatory Requirements</strong></p>
<p>For IVD diagnostic use or regulatory submissions, confirm the kit's current regulatory status. The Quick-DNA/RNA Viral MagBead Kit has been used in EUA workflows; its current authorizations and intended uses may have evolved since initial authorization. Always verify directly with Zymo Research or MBP's regulatory affairs team for current compliance documentation.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>The Quick-DNA/RNA Viral MagBead Kit binds small (&gt;50 nt) and large (&gt;200 kb) DNA and RNA to magnetic beads and elutes high-quality nucleic acids ready for NGS, hybridization-based detection, and RT-qPCR. The kit is used in Zymo Research's SARS-CoV-2 EUA extraction workflow and has been adopted in published multiplex respiratory panel methods and pan-pathogen metagenomics protocols. A published PMC study on integrated DNA and RNA extraction from viral pathogens causing acute respiratory infections demonstrated that magnetic bead-based co-extraction achieved equivalent or superior sensitivity to spin-column formats for both RSV (RNA virus) and adenovirus (DNA virus) from the same nasopharyngeal swab input. Magnetic bead viral co-extraction is the standard format for large public health reference laboratories and clinical metagenomics programs processing 96-1,500+ samples per day across outbreak response and surveillance networks.</p>
<p dir="ltr">&nbsp;</p>
<p dir="ltr">Contact the helpful<strong> <a href="https://mbpinc.net/contact-us">team at MBP</a> </strong>today to request a quote for your specific research requirements.</p>

Mag Beads

MSDS

R2140-MSDS

R2140-Protocol

Quick-DNA/RNA Viral MagBead

<p>R2140 by Zymo Research is the Quick-DNA/RNA&trade; Viral MagBead kit, a 250-prep magnetic bead system that simultaneously isolates DNA and RNA from up to 200 &micro;l of plasma, serum, urine, saliva, whole blood, cell culture media, or swab samples. Elution volume is 30 &micro;l (standard); binding capacity is 5 &micro;g DNA/RNA per 10 &micro;l of MagBinding Beads. Size range is 50 nucleotides to ~200 kb. All steps run at room temperature.</p>
<p>Compatible with KingFisher, Hamilton, and Tecan automated liquid-handling platforms. A CE-IVD version (R2140-E) is available. Related: R2141 (1,000 preps).</p>
<p>MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.</p>

Yield

Quick-DNA/RNA Viral MagBead (250 Preps.)

Quick-DNA/RNA™ Viral MagBead - DX (250 Preps.)

Quick-DNA/RNA Viral MagBead (1000 Preps.)

Quick-DNA/RNA Viral MagBead - DX (1000 Preps)

Load N’ Go™ DNA/RNA Viral Kit

<h2>What are Spin-Column Viral DNA/RNA Co-Extraction Kits?</h2>
<p>&nbsp;</p>
<p>Spin-column viral DNA/RNA co-extraction kits purify viral DNA and RNA simultaneously from plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media using a single-buffer lysis that disrupts viral particles and conditions nucleic acids to bind a Zymo-Spin silica-membrane column in a standard microcentrifuge. Both DNA and RNA elute together in 30-100 ul of DNase/RNase-free water, ready for multiplex RT-qPCR targeting RNA viruses (SARS-CoV-2, influenza A/B, RSV, HIV) and DNA viruses (adenovirus, herpesvirus, HPV, parvovirus) from the same eluate in a single assay. Choose the spin-column viral DNA/RNA format when processing fewer than 50 samples per run, when only a microcentrifuge is available, or when the fastest possible turnaround from sample to detectable eluate is required for point-of-care adjacent testing.</p>
<p dir="ltr">&nbsp;</p>
<h2 dir="ltr">What you will find:</h2>
<p dir="ltr">&nbsp;</p>
<ul>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Quick-DNA/RNA&trade; Viral Kit (D7020): Efficient spin-column technology designed for the fast co-purification of high-quality viral RNA and viral DNA from liquid specimens.</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">Virus RNA Kit (R6620-00): Specialized silica-membrane columns created for sensitive extraction of viral RNA, guaranteeing pure yields for subsequent detection processes.</p>
</li>
<li dir="ltr" aria-level="1">
<p dir="ltr" role="presentation">MV Total RNA Isolation (R6313-01): Custom extraction columns designed for the retrieval of total RNA from difficult molecular biology samples, ensuring reliable recovery.</p>
</li>
</ul>
<p>&nbsp;</p>
<h2>How to Choose a Spin-Column Viral DNA/RNA Co-Extraction Kit</h2>
<p>&nbsp;</p>
<p><strong>Single-Buffer vs. Multi-Buffer Lysis</strong></p>
<p>Zymo Quick-DNA/RNA Viral Kit uses a single-step lysis buffer -- no protease incubation, no carrier RNA addition, no ethanol mixing before column loading. This reduces protocol complexity and per-batch error rate. Multi-buffer kits (QIAGEN QIAamp, Thermo PureLink) require preparation of lysis buffer + carrier RNA + ethanol before loading; they are comparable in yield but add setup time per batch.</p>
<p><strong>Input Volume Flexibility</strong></p>
<p>Standard spin-column viral kits accept 50-200 ul of cell-free sample. For low-titer samples requiring larger input volumes to increase sensitivity, check the kit for extended-input protocols; some kits allow 400-1,000 ul plasma with vacuum manifold loading. For samples in the DNA/RNA Shield, direct loading is validated without dilution.</p>
<p><strong>DNA+RNA vs. RNA-Only Recovery</strong></p>
<p>Confirm you need co-extraction before ordering. If your panel includes only RNA viral targets (influenza, RSV, SARS-CoV-2), an RNA-only viral spin-column kit produces equivalent sensitivity with a simpler protocol. Co-extraction is essential when your panel includes HPV, adenovirus, CMV, EBV, parvovirus, or any other DNA virus target - RNA-only kits will under-recover or miss these targets.</p>
<p><strong>Elution Volume and Downstream Assay</strong></p>
<p>Elute in 30-50 ul for RT-qPCR sensitivity; the higher nucleic acid concentration produces Ct improvement of 0.5-1.5 cycles compared to 100 ul elution. For multiplex NGS library preparation, elute in 100 ul and quantify by Qubit dsDNA BR and RNA HS assays separately before library input normalization.</p>
<p><strong>Throughput Scaling</strong></p>
<p>When daily volumes exceed 24 samples, the spin-column format creates centrifuge bottlenecks. At &gt;50 samples per run, transition to the Quick-DNA/RNA Viral 96-well plate format (vacuum manifold) or Quick-DNA/RNA Viral MagBead format (automation) for higher throughput.</p>
<p>&nbsp;</p>
<h2>Specifications Context</h2>
<p>&nbsp;</p>
<p>Performance benchmarks for spin-column viral DNA/RNA co-extraction: &gt;=70% recovery of RNA and DNA spike-in controls, Ct within 2 cycles of reference extraction for validated viral targets, and A260/A280 &gt;= 1.7 in eluates. The Zymo Quick-DNA/RNA Viral Kit uses a single buffer system validated for enteroviruses, rhinoviruses, coronaviruses (SARS-CoV-2), HIV, HCV, influenza A, flaviviruses (dengue, Zika), measles, parainfluenza, and parvovirus; the kit is used in Zymo's SARS-CoV-2 extraction workflow and has been adopted in published multiplex respiratory panel methods. Spin-column viral co-extraction kits are the standard format in clinical research labs processing 10-50 patient samples per run for research-use multiplex RT-qPCR panels at institutions including Vanderbilt University and MD Anderson Cancer Center.</p>
<p>&nbsp;</p>
<p>For kit recommendations, bulk pricing on Quick-DNA/RNA Viral spin-column kits, or guidance on pairing with multiplex RT-qPCR panels,&nbsp;<a href="https://mbpinc.net/contact-us"><strong>contact the MBP team</strong></a>. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.</p>

Spin-Column

MSDS - D7020-1

Quick-DNA/RNA™ Viral Kit

<p>D7020 by Zymo Research co-isolates viral DNA and RNA from up to 400 µL of plasma, serum, urine, blood, saliva, or swab samples in ~5–10 minutes hands-on time. The included DNA/RNA Shield™ 2X lyses viral particles, inactivates infectious agents, and stabilizes nucleic acids at ambient temperature—supporting safe room-temperature sample transport. Eluate is ready for NGS, RT-qPCR, and hybridization detection.</p>