DNA/RNA Co-Extraction Kits

<h2>What are Cell-Free DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Cell-free DNA/RNA co-extraction kits simultaneously isolate circulating cell-free DNA (cfDNA -- ctDNA fragments of 130-250 bp, apoptotic DNA) and cell-free RNA (cfRNA -- microRNAs, lncRNAs, exosomal RNA, circulating mRNA fragments) from the same plasma, serum, or urine sample without phenol-chloroform or protein precipitation, delivering both analytes for parallel liquid biopsy genomic and transcriptomic analysis from a single 1-4 ml blood draw. Unlike dedicated cfDNA kits that miss circulating RNA species, or cfRNA kits that under-recover short cfDNA fragments, co-extraction kits are formulated to bind both nucleic acid classes - including protein-bound RNA, exosomal RNA, and miRNA - from cell-free matrices where total nucleic acid concentration is 1-100 ng/ml. Use a cfDNA/cfRNA co-extraction kit when your liquid biopsy workflow requires both somatic mutation analysis (ctDNA sequencing) and RNA expression profiling (circulating miRNA or cfRNA-seq) from the same blood draw, or when sample volume is limited and splitting between separate DNA and RNA kits would leave insufficient input for either.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-cfDNA/cfRNA&trade; Serum &amp; Plasma Kit: An efficient spin-column setup tailored for sample volumes up to 3 ml, providing reduced sequence bias and recovering as much as 515 times more microRNA.
</li>
<li dir="ltr" aria-level="1">
High-Purity Elution Technology: Nuclease-free methods enabling the separate or co-elution of nucleic acids, ensuring transfection-grade purity for delicate bioinformatics and RT-qPCR uses.
</li>
</ul>
&nbsp;
<h2>How to Choose a cfDNA/cfRNA Co-Extraction Kit</h2>
&nbsp;
Input Volume and Assay Sensitivity
cfDNA concentration in cancer patient plasma ranges from 1-1,000 ng/ml depending on tumor stage and type; circulating miRNA concentration is typically 1-100 pg/ml. For low-MAF ctDNA detection (&lt;0.1%), 2-4 ml plasma input is required to obtain sufficient copies for ddPCR or ultra-deep sequencing. For cfRNA profiling only, 200 &mu;L-1 mL is sufficient for miRNA RT-qPCR panels. The Zymo Quick-cfDNA/cfRNA Kit scales linearly up to 3 ml input.
Co-Elution vs. Separate Fractions
The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit offers the option to co-elute DNA and RNA into a single fraction or to collect separate fractions. Co-elution maximizes nucleic acid recovery in the smallest volume -- optimal for low-abundance targets; separate fractions are needed when DNA-specific (bisulfite sequencing, whole-exome) and RNA-specific (rRNA depletion, miRNA-seq library) downstream protocols cannot use the same input.
miRNA Recovery
Circulating miRNAs (18-22 nt) are small, low-abundance, and easily lost during standard DNA extraction. Confirm the co-extraction kit explicitly recovers small RNAs &lt;200 nt from plasma. The Zymo Quick-cfDNA/cfRNA Kit recovers up to 515x more microRNA than conventional phase-separation methods -- a validated difference confirmed in the Zymo product datasheet.
Phenol-Free Chemistry
For high-throughput liquid biopsy programs, phenol-free kits eliminate fume-hood requirements and toxic waste handling. The QIAGEN ccfDNA/RNA Kit and Zymo Quick-cfDNA/cfRNA Kit are both phenol-free; automated protocols using these kits can process large cohort batches without hazardous chemical handling steps.
Pre-Analytical Standardization
cfDNA/cfRNA concentrations vary with blood collection tube type, time-to-processing, and centrifugation protocol. Standardize on EDTA tubes + two-spin plasma preparation (300xg then 16,000xg) and process within 6 hours of collection or store at -80 degrees C. For samples requiring extended transport, Streck Cell-Free DNA BCT or cfRNA preservation tubes stabilize both analytes at room temperature for up to 7 days before extraction.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-cfDNA/cfRNA Serum &amp; Plasma Kit enables simple spin-column isolation of all circulating cell-free DNA and RNA from serum, plasma, and biological fluids, recovering protein-bound, exosomal, miRNA, and other small RNA in addition to cfDNA. Cell-free nucleic acids scale linearly with input volume up to 3 ml. A 2022 digital PCR comparative study (PMC9805675) evaluated multiple cfDNA/cfRNA co-purification kits across plasma input volumes of 0.06-4 ml, finding that combined cfDNA/cfRNA quantification produces higher total nucleic acid concentrations than parallel separate quantification. cfDNA/cfRNA multi-omic liquid biopsy is an active area of clinical translational research for non-invasive cancer diagnosis and treatment monitoring, with studies underway at MD Anderson Cancer Center and Vanderbilt University using co-extraction formats to maximize information per blood draw.
&nbsp;
Close the gap between liquid biopsy and groundbreaking discovery&mdash;<a href="https://mbpinc.net/contact-us">reach out to MBP Inc.</a> now to obtain a quote for your next-generation sequencing requirements.

Cell-free DNA/RNA

MSDS

DataSheet

Protocol

Quick-cfDNA/cfRNA™ Serum & Plasma Kit (50 Preps.)

Quick-cfDNA/cfRNA™ Serum &amp; Plasma Kit (R1072) by Zymo Research — 50 preps. Isolates cfDNA (≥50 bp) and cfRNA/miRNA (≥17 nt) from up to 3 ml serum, plasma, CSF, urine, or biological fluids. Elution 6–15 µl. Up to 515× more microRNA recovery. Co-purification (~30 min) or parallel purification modes.

Elution Volume

Processing Time

Purity

Required Equipment

Sample Types

Size

Yield

Manufacturer

Shipping Condition

Storage Condition

<h2>What are Magnetic Bead Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Magnetic bead microbial DNA/RNA co-extraction kits purify both genomic DNA and total RNA, including small RNAs &gt;=17 nt, from microbial community samples - stool, soil, water, biofilm, swabs, and body fluids using BashingBead mechanical lysis in a 96-deep-well plate followed by binding of nucleic acids to paramagnetic silica-coated beads, magnetic separation during successive wash steps to remove PCR inhibitors, and elution in &gt;=50 ul of ZymoBIOMICS DNase/RNase-Free Water. The entire purification phase requires no centrifugation and integrates directly with KingFisher Flex, Accuris IsoPure 96, and AllSheng AutoPure 96 liquid-handling platforms. Choose the magnetic bead format for microbial DNA/RNA co-extraction when processing 48+ community samples per session, when a compatible automation platform is available, or when reproducibility across hundreds of samples is required for a human microbiome cohort, environmental monitoring, or clinical trial.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA HT Kit for high-throughput isolation of total nucleic acids from liquid clinical and biological specimens using a magnetic bead&ndash;based extraction system.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS MagBead DNA/RNA Kit for unbiased recovery of microbiome DNA and RNA without chemical bias, featuring an innovative lysis system.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA Pathogen MagBead Kit for rapid isolation of viral, bacterial, and protozoan nucleic acids from challenging vector samples using an optimized buffer
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Automation Script Availability
Confirm the kit manufacturer provides a validated, instrument-specific script for your platform before purchase. Zymo Research provides automation scripts for KingFisher Flex, KingFisher Apex, Accuris IsoPure 96, and AllSheng AutoPure 96; Tecan Fluent and Hamilton STAR scripts require adaptation. Script development and validation add 2-4 weeks; factor this into project timelines.
Combined vs. Separate Analyte Fractions
The ZymoBIOMICS MagBead DNA/RNA kit elutes a combined total nucleic acid fraction. For studies requiring separate DNA and RNA fractions from the same extraction (parallel metagenomics and metatranscriptomics), apply post-extraction enzymatic depletion (DNase I on the RNA aliquot, RNase A on the DNA aliquot) or use the ZymoBIOMICS DNA/RNA Miniprep kit, which uses column-switching architecture for native separation.
96-Well Bead-Beating Setup
The lysis step requires a 96-well bead mill compatible with ZR-96 BashingBead Lysis Racks. Validated instruments include GenoGrinder 2000, Retsch MM400 with a 96-tube rack adapter, and Bertin Precellys. The bead-beating step is the primary manual step in an otherwise automated workflow; confirm the lab has a compatible bead mill before adopting the kit.
Inhibitor Load by Sample Type
Stool samples have moderate inhibitor loads (bile salts, starch metabolites) that ZymoBIOMICS wash steps reliably remove. Soil samples -- especially high-humic tropical or peat soils - carry heavier inhibitor loads that may require reducing input mass to 10-50 mg per well and validating A260/A230 &gt;= 1.5 on initial test extractions. If A260/A230 remains below 1.5, apply an additional cleanup step with Zymo OneStep PCR Inhibitor Removal Columns before library preparation.
DNA/RNA Shield Sample Collection
For multi-site human microbiome cohort studies, use DNA/RNA Shield fecal collection tubes (Zymo R1101) as the collection device. Samples stabilized at room temperature for up to 30 days in Shield are directly loadable into the ZR-96 BashingBead Rack, streamlining the pre-extraction workflow and enabling consistent RNA preservation across diverse collection sites.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
ZymoBIOMICS MagBead DNA/RNA kit produces total nucleic acid eluted into &gt;=50 ul with A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and DNA fragment sizes suitable for 16S rRNA amplicon sequencing and shotgun metagenomics. Input specification: approximately 2 &times; 10^8 bacterial cells, 2 &times; 10^7 yeast cells, or ~40 mg human stool per well. A published 2024 high-throughput fecal microbiome DNA extraction study demonstrated that bead-beating at &gt;=3,000xg significantly improves detection of hard-to-lyse Gram-positive genera - Blautia, Bifidobacterium, and Ruminococcus compared with no bead-beating, confirming that mechanical lysis is non-negotiable for unbiased community co-extraction. ZymoBIOMICS MagBead workflows are used in NIH-funded human microbiome research and are the standard extraction approach for multi-site gut microbiome cohort studies at institutions registered with MBP, including Howard Hughes Medical Institute and MD Anderson Cancer Center.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> and get high-yield mag bead systems for your high-throughput extraction workflows.

Mag Beads

MSDS (R2150-E/R2151-E)

MSDS (R2150/R2151)

R2152-MSDS

Protocol (R2150/R2151)

Protocol (R2150-E/R2151)

R2152-Protocol

Quick-DNA/RNA HT

The Quick-DNA/RNA&#8482; HT kit by Zymo Research is a magnetic bead-based system for simultaneous co-purification of total DNA and RNA from swabs, blood, plasma, serum, saliva, tissue, and samples in transport media (UTM, VTM, DNA/RNA Shield, PAXgene). Binding capacity of 5 µg per 10 µl HT MagBinding Beads, elution in ≥15 µl, with no centrifugation required — fully compatible with automated platforms. Available as R2150 (250 preps), R2150-E (250 preps, CE-IVD), R2151 (1,000 preps), and R2151-E (1,000 preps, CE-IVD).

Binding Capacity

Sample Sources

Storage Temperature and Stability

Quick-DNA/RNA™ HT (250 preps)

Quick-DNA/RNA™ HT - Dx (250 preps)

Quick-DNA/RNA™ HT (1000 preps)

Quick-DNA/RNA™ HT - Dx (1000 preps)

<h2>What are Spin-Column DNA/RNA Co-Extraction Kits for Cells, Tissue, and Blood?</h2>
&nbsp;
Spin-column DNA/RNA co-extraction kits for cells, tissue, and blood simultaneously purify genomic DNA and total RNA in separate fractions from the same mammalian sample using a sequential two-column silica-membrane workflow in a standard microcentrifuge. The Zymo Quick-DNA/RNA Miniprep Plus Kit loads the sample lysate onto a Zymo-Spin IIICG column that physically captures gDNA while RNA passes through to a Zymo-Spin IIC column for binding, washing, and elution - completing both DNA and RNA purification in under 15 minutes for cell pellets and 45-75 minutes (including Proteinase K digestion) for solid tissue biopsies. Choose the spin-column format for DNA/RNA co-extraction from cells, tissue, and blood when processing 1-24 samples per run, when automation infrastructure is not available, or when the fastest possible DNA + RNA turnaround from a limited biopsy sample is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Miniprep Kit for rapid co-extraction of total DNA and RNA from blood and tissues utilizing advanced spin-column technology
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Microprep Plus Kit to recover high-purity nucleic acids from extremely small or rare samples using a specialized micro-elution column
</li>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA Miniprep Plus Kit to ensure high-yield genomic DNA and total RNA yields using an ultra-clean spin-column workflow
</li>
<li dir="ltr" aria-level="1">
Blood RNA Mini Kit for the specific extraction of intact viral and total host RNA from whole blood samples using robust silica-membrane columns
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column DNA/RNA Co-Extraction Kit for Cells, Tissue, and Blood</h2>
&nbsp;
Miniprep Plus vs. Microprep Plus Format
The Miniprep Plus kit handles standard inputs (up to 5x10^6 cells or 30 mg tissue) and elutes in 25-100 ul per fraction, appropriate for most downstream applications. The Microprep Plus kit is designed for very low inputs -- 500 cells to 5,000 cells or 0.5-5 mg tissue -- with minimum elution volumes of 6-10 ul per fraction for maximum concentration from limited material. Use Microprep Plus for needle biopsy cores, laser-capture microdissected cells, single-well organoid lysates, and sorted immune cell populations.
DNA/RNA Shield for Sample Lysis and Stabilization
DNA/RNA Shield in the Miniprep Plus and Microprep Plus kits performs dual duty: it lyses cells on contact while simultaneously stabilizing DNA and RNA at room temperature, enabling sample collection and processing to be decoupled by hours or days without cold-chain handling. For tissue biopsies collected in the operating room that cannot be processed immediately, submerging in DNA/RNA Shield preserves RNA integrity for up to 30 days at room temperature -- the practical alternative to snap-freezing in liquid nitrogen.
Tissue Digestion Strategy
Soft, high-cellularity tissues (liver, spleen, kidney cortex) lyse completely in DNA/RNA Shield + Proteinase K at 55 degrees C within 30 minutes. Fibrous tissues (skeletal muscle, heart, skin, lung) require 1-2 hours of Proteinase K digestion or mechanical homogenization (bead-beating, tissue grinder) before column loading. For very fibrous tissue that resists Proteinase K digestion, add bead-beating in a lysis tube (0.5 mm beads) with DNA/RNA Lysis Buffer before proceeding to column loading.
On-Column DNase I for the RNA Fraction
Always include on-column DNase I treatment for RNA-seq and for RT-qPCR assays without intron-spanning primers. Skip DNase I only for RT-qPCR with exon-spanning primer pairs that distinguish cDNA from gDNA amplicons. The IIICG column physically depletes the majority of gDNA but does not eliminate 100%; residual gDNA in the RNA fraction produces false-positive amplification in RT-qPCR assays without an intron-spanning design.
Elution Strategy for Downstream Applications
For RNA-seq: elute RNA in 50-100 ul, quantify by Qubit RNA HS Assay, and verify RIN &gt;= 7 on a Bioanalyzer before library preparation. For RT-qPCR: elute in 25-50 ul for higher RNA concentration. For gDNA applications requiring &gt;= 5 kb fragments (WGS, WES, long-read sequencing): elute DNA in 50-100 ul and quantify by Qubit dsDNA BR Assay; avoid vortexing gDNA eluates to preserve high-molecular-weight fragment integrity.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA Miniprep Plus Kit produces gDNA (A260/A280 &gt;= 1.8, high molecular weight &gt;= 20 kb) and total RNA, including small RNAs &gt;=17 nt (A260/A280 &gt;= 1.8, RIN &gt;= 7 from fresh tissue) in under 15 minutes from cell pellets, with both analytes ready for next-generation sequencing without further cleanup. The Quick-DNA/RNA Miniprep Plus Kit is among the most widely used spin-column co-extraction kits in academic molecular biology, gene therapy, pharmacology, and cancer multi-omics research; its use is cited in published studies at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center for paired genomic and transcriptomic analyses. For needle biopsy or LCM inputs where the Miniprep Plus minimum input cannot be met, the Microprep Plus format recovers both analytes from the single-cell level without carrier RNA or pre-amplification requirements.
&nbsp;
For guidance on selecting the right spin-column co-extraction format for your input type or for pricing, <a href="https://mbpinc.net/contact-us">contact the expert team at MBP Inc. </a>

Spin-column

MSDS - D7003

MSDS - D7003T

Quick-DNA/RNA Miniprep Plus Kit

The Quick-DNA/RNA Miniprep Plus Kit (D7003) by Zymo Research isolates both high-molecular-weight DNA (≥40 kb) and total RNA including miRNA (≥17 nt) simultaneously from a single sample, supporting FFPE, blood, cells, and tissue in 50 preps with DNase I, Proteinase K, and DNA/RNA Shield included. Available in 10 preps (D7003T) and 50 preps (D7003).

Equipment

Sample Source

Size Range

Quick-DNA/RNA MiniPrep Plus Kit (50 Preps.)

Quick-DNA/RNA™ MiniPrep Plus Kit (10 Preps.)

R2145-Protocol

Quick-DNA/RNA Pathogen MagBead

Quick-DNA/RNA Pathogen MagBead by Zymo Research — magnetic bead co-purification of DNA and RNA from insect vectors (mosquitoes, ticks, fleas), tissue, and biological liquids. Binding capacity 10 µg/20 µl beads; ≥50 µl elution. Available as R2145 (96 preps) and R2146 (4×96 preps).

Equipment Needed

Materials

Sample Type

Quick-DNA/RNA™ Pathogen MagBead (96 Preps.)

Quick-DNA/RNA™ Pathogen MagBead (4 x 96 Preps.)

<h2>What are 96-Well Plate Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
96-well plate viral DNA/RNA co-extraction kits purify both viral DNA and RNA from up to 96 cell-free samples simultaneously - plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media -- using the same single-buffer lysis chemistry as spin-column viral kits but operating in a Zymo-Spin I-96 spin plate processed with a 96-well plate centrifuge or vacuum manifold. Both analytes co-elute per well in DNase/RNase-free water covering RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, HPV, parvovirus) without requiring robotic automation. The format provides a significant throughput step-up from single-tube spin columns - processing 96 samples in under 30 minutes with a multichannel pipette and standard centrifuge. Choose the 96-well plate format when daily viral sample volumes exceed 24 per run, when automation infrastructure is unavailable, or when batch surveillance testing requires rapid plate-scale turnaround from clinical specimens.
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<h2 data-start="0" data-end="23">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA/RNA Viral 96 Kit (D7022)&nbsp;for concurrent isolation of viral DNA and viral RNA from fluid specimens.</li>
<li>96-well plate format for high-throughput sample processing.</li>
<li>Rapid purification workflow for efficient nucleic acid recovery.</li>
<li>Designed for processing multiple samples simultaneously.</li>
<li>Purified viral nucleic acids suitable for downstream molecular biology applications.</li>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>
</section>
</div>
&nbsp;
<h2>How to Choose a 96-Well Plate Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Centrifuge vs. Vacuum Manifold Operation
A 96-well plate centrifuge rotor (accommodating the Zymo-Spin I-96 Plate dimensions) is the standard equipment requirement. If a compatible plate centrifuge is unavailable, a vacuum manifold that fits 96-well spin plates provides equivalent performance and eliminates centrifuge dependency. Confirm equipment compatibility before kit purchase.
Manual vs. Semi-Automated Pipetting
The 96-well format is designed for manual operation with multichannel pipettes for buffer addition and sample loading. Semi-automation is achievable with a liquid handler for buffer dispensing steps, leaving centrifugation as the manual step. For full automation without centrifugation, use the Quick-DNA/RNA Viral MagBead format on a compatible robot.
Lysis Buffer Volume Per Well
The single-buffer lysis system adds 200 ul of lysis buffer per well of sample (1:1 v/v for 200 ul input). Ensure the plate's well volume accommodates the combined lysis buffer + sample volume (400 ul total per well) plus ethanol addition before loading. Most Zymo-Spin I-96 plate protocols include a deep-well collection plate for this mixing step.
Positive and Negative Control Layout
Reserve wells 11 and 12 of each row for extraction controls. Fixed control placement across all plates enables automated data review scripts to flag failed wells based on consistent control positions.
Throughput Scaling Beyond 96
For programs processing 192-384 samples per day, run two 96-well plates sequentially on the same centrifuge or vacuum manifold. For volumes above 384 per day, transition to the magnetic bead format on a liquid handler (KingFisher, Tecan) to process 96 samples per automated run without per-batch manual centrifugation steps.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and DNA/RNA adsorption onto the Zymo-Spin I-96 Plate matrix, with validated recovery of enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A, flaviviruses, measles, parainfluenza, and parvovirus. Nucleic acids are eluted in DNase/RNase-free water suitable for RT-PCR, NGS, and hybridization applications. 96-well plate viral extraction is the standard format in public health and reference laboratory surveillance programs, processing 96-384 clinical specimens per day, including respiratory multiplex panels, arboviral surveillance, and bloodborne pathogen screening, with throughput requirements driving adoption of this format over single-tube spin columns.
&nbsp;
For bulk pricing on Quick-DNA/RNA Viral 96 kits, centrifuge compatibility checks, or guidance on pairing with validated multiplex RT-qPCR assays,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

96-well plate

MSDS - D7022

D7023

Quick-DNA/RNA Viral 96 Kit

The Quick-DNA/RNA™ Viral 96 Kit is available in the following variants: D7022, D7023.The Quick-DNA/RNA Viral 96 Kit (D7022) by Zymo Research simultaneously recovers viral DNA and RNA in a 96-well format from plasma, serum, saliva, urine, blood, and other samples — yielding up to 5 µg DNA and 10 µg RNA per prep in DNase/RNase-free water. No organic denaturants or proteases; eluate is directly compatible with RT-PCR, qPCR, and NGS.

Quick-DNA/RNA™ Viral 96 Kit (2 x 96)

Quick-DNA/RNA™ Viral 96 Kit (4 x 96 Preps.)

<h2>What are Magnetic Bead Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Magnetic bead viral DNA/RNA co-extraction kits purify both viral DNA and RNA from plasma, serum, urine, nasopharyngeal swabs, saliva, blood, cellular suspensions, biopsies, fecal samples, and cell culture media stored in DNA/RNA Shield using paramagnetic silica-coated beads that bind nucleic acids from &gt;50 nt to &gt;200 kb during a single chaotropic lysis step, with magnetic separation during successive wash steps removing inhibitors - no centrifugation required during purification. The 96-well format integrates with KingFisher Flex, Tecan Fluent, and Hamilton STAR liquid-handling platforms, enabling fully automated extraction of both RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, herpesvirus, parvovirus) from 96 samples per run. Choose the magnetic bead format for viral DNA/RNA co-extraction when processing 48+ samples per run, when a compatible automation platform is available, or when the program requires EUA-associated workflows validated for SARS-CoV-2 or other respiratory panel targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Magbio HighPrep Viral-Bacterial DNA &amp; RNA Kit (HVB-DR0): Scalable magnetic bead chemistry optimized for the simultaneous extraction of viral and bacterial nucleic acids from diverse fluid profiles.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral DNA &amp; RNA Kit (HPV-DR0): High-efficiency magnetic isolation systems engineered for the rapid purification of viral target vectors from fluid specimens.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral-Pathogen DNA &amp; RNA Kit (HPP-DR0): Specialized bead technology designed to maximize pathogen recovery and eliminate inhibitors from challenging biological matrices.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Quick-DNA/RNA Viral MagBead (R2140): Advanced magnetic-bead separation configurations built for fast, high-purity co-purification of total viral nucleic acids.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Automation Platform Compatibility
Confirm the kit's automation scripts are available for your specific platform before purchase. The Quick-DNA/RNA Viral MagBead Kit is used in Zymo Research's SARS-CoV-2 EUA workflow and is compatible with KingFisher and other major liquid-handling platforms; contact MBP's technical team or Zymo Research's automation support for current script availability. For Tecan Fluent or Hamilton STAR, platform-specific script development or adaptation may be required.
DNA/RNA Shield as the Collection Medium
The Quick-DNA/RNA Viral MagBead Kit is designed around DNA/RNA Shield as the sample collection, inactivation, and storage medium. Swabs or other samples stabilized in DNA/RNA Shield load directly into the extraction protocol without additional neutralization steps. For programs using VTM (viral transport medium), UTM, or PBS as collection media, confirm compatibility with the kit's lysis buffer by testing spike-in recovery before adopting at scale.
Carrier RNA Requirement
Unlike the QIAGEN QIAamp Viral RNA Mini Kit, the Quick-DNA/RNA Viral MagBead Kit does not require the user to prepare and add carrier RNA to the lysis buffer - the kit's buffer system is formulated for complete lysis and efficient nucleic acid binding without this step. Eliminating the carrier RNA preparation step reduces per-batch setup time and removes one source of inter-batch variability in high-throughput automated workflows.
Nucleic Acid Size Recovery Range
Confirm the kit recovers the size range needed for your downstream application. The Quick-DNA/RNA Viral MagBead Kit binds nucleic acids from &gt;50 nt to &gt;200 kb - covering small viral RNAs (miRNA-sized fragments from viral genomes), standard RT-qPCR amplicon targets, and full-length viral genomes for metagenomic NGS. Applications requiring recovery of very short fragments (&lt;50 nt) need a kit specifically validated for that range.
Regulatory Requirements
For IVD diagnostic use or regulatory submissions, confirm the kit's current regulatory status. The Quick-DNA/RNA Viral MagBead Kit has been used in EUA workflows; its current authorizations and intended uses may have evolved since initial authorization. Always verify directly with Zymo Research or MBP's regulatory affairs team for current compliance documentation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Quick-DNA/RNA Viral MagBead Kit binds small (&gt;50 nt) and large (&gt;200 kb) DNA and RNA to magnetic beads and elutes high-quality nucleic acids ready for NGS, hybridization-based detection, and RT-qPCR. The kit is used in Zymo Research's SARS-CoV-2 EUA extraction workflow and has been adopted in published multiplex respiratory panel methods and pan-pathogen metagenomics protocols. A published PMC study on integrated DNA and RNA extraction from viral pathogens causing acute respiratory infections demonstrated that magnetic bead-based co-extraction achieved equivalent or superior sensitivity to spin-column formats for both RSV (RNA virus) and adenovirus (DNA virus) from the same nasopharyngeal swab input. Magnetic bead viral co-extraction is the standard format for large public health reference laboratories and clinical metagenomics programs processing 96-1,500+ samples per day across outbreak response and surveillance networks.
&nbsp;
Contact the helpful <a href="https://mbpinc.net/contact-us">team at MBP</a> today to request a quote for your specific research requirements.

R2140-MSDS

R2140-Protocol

Quick-DNA/RNA Viral MagBead

R2140 by Zymo Research is the Quick-DNA/RNA&trade; Viral MagBead kit, a 250-prep magnetic bead system that simultaneously isolates DNA and RNA from up to 200 &micro;l of plasma, serum, urine, saliva, whole blood, cell culture media, or swab samples. Elution volume is 30 &micro;l (standard); binding capacity is 5 &micro;g DNA/RNA per 10 &micro;l of MagBinding Beads. Size range is 50 nucleotides to ~200 kb. All steps run at room temperature.
Compatible with KingFisher, Hamilton, and Tecan automated liquid-handling platforms. CE-IVD versions (R2140-E, R2141-E) and a 1,000-prep format (R2141) are available, along with a pre-filled 96-well Load N’ Go format (R2143).
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-DNA/RNA Viral MagBead (250 Preps.)

Quick-DNA/RNA™ Viral MagBead - DX (250 Preps.)

Quick-DNA/RNA Viral MagBead (1000 Preps.)

Quick-DNA/RNA Viral MagBead - DX (1000 Preps)

Load N’ Go™ DNA/RNA Viral Kit

<h2>What are FFPE DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
FFPE DNA/RNA co-extraction kits simultaneously isolate genomic DNA and total RNA -- including small RNAs &gt;=17 nt from the same formalin-fixed paraffin-embedded tissue section using xylene-free deparaffinization, Proteinase K digestion, heat-mediated formaldehyde crosslink reversal, and Zymo-Spin column purification. Co-extraction from a single undivided FFPE section conserves precious archival material and ensures both DNA (for somatic mutation calling, CNV analysis, methylation profiling) and RNA (for expression profiling, fusion detection, nCounter panels) originate from identical tissue, eliminating the spatial heterogeneity introduced when different tissue sections or block portions are used for each analyte. Use an FFPE DNA/RNA co-extraction kit whenever archival tissue is limited, as in needle biopsy cores, small surgical biopsies, or rare tumour specimens, or when integrated multi-omic analysis (paired WES + RNA-seq, or mutation + expression correlation) is required from the same pathological specimen.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; FFPE MiniPreps: Efficient systems designed to extract high-quality total nucleic acids with reduced sequence bias, tailored for subsequent qPCR and NGS library preparation.
</li>
<li dir="ltr" aria-level="1">
Decross-linking Agents: Nuclease-free solutions formulated to effectively liberate nucleic acids from protein complexes, ensuring transfection-grade purity for delicate bioinformatics workflows.
</li>
</ul>
&nbsp;
<h2>How to Choose an FFPE DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Xylene-Free vs. Xylene-Based Deparaffinization
Zymo Research Quick-DNA/RNA FFPE Miniprep Kit uses a proprietary Deparaffinization Solution that removes paraffin without xylene, making it safer for labs without fume hoods and eliminating toxic waste disposal requirements.
Tissue Section vs. Tissue Block Curling
For formalin-fixed paraffin-embedded sections on slides, cut and scrape sections directly into the Deparaffinization Solution tube - 2-4 sections of 5-10 um thickness is the standard starting point. For tissue blocks, cut 10-20 um sections with a microtome into a 1.5 ml tube. Avoid over-cutting; excessive paraffin volume from thick sections delays deparaffinization and reduces proteinase K efficiency, leading to lower DNA and RNA yields.
DNA and RNA Fraction Separation
The Zymo Quick-DNA/RNA FFPE Miniprep Kit delivers non-crosslinked DNA and DNA-free total RNA in separate fractions, making it suitable for downstream molecular analyses from the same FFPE sample.
Amplicon Size for Downstream Assays
RNA isolated from FFPE tissue is typically fragmented because of formalin fixation. For RT-qPCR, primer pairs producing amplicons of approximately 70&ndash;150 bp generally provide the most reliable amplification. The purified RNA from the Quick-DNA/RNA&trade; FFPE MiniPrep is suitable for downstream applications including RT-PCR, next-generation sequencing (NGS), microarray analysis, and hybridization, provided library preparation and assay workflows are compatible with fragmented FFPE-derived nucleic acids.
Block Age Assessment
Request pathology records for fixation time and block age before extraction. For blocks &gt;10 years old, perform a diagnostic RT-qPCR on a short amplicon (60-80 bp) reference gene in the RNA fraction before committing to full RNA-seq library preparation. RNA from blocks with Ct &gt;35 for abundant reference genes (ACTB, GAPDH) at short amplicons typically fails RNA-seq quality thresholds.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA&trade; FFPE Miniprep Kit delivers non-crosslinked DNA and DNA-free total RNA (including small RNAs &ge;17 nt) from FFPE tissue using a xylene-free deparaffinization workflow. The purified nucleic acids are suitable for downstream applications including PCR, RT-PCR, next-generation sequencing (NGS), hybridization, and other molecular analyses compatible with FFPE-derived samples. Because formalin fixation fragments nucleic acids, downstream assays are generally designed with short amplicons (approximately 60&ndash;150 bp for RNA and 100&ndash;300 bp for DNA) to improve amplification efficiency. Co-isolation of DNA and RNA from the same FFPE specimen supports integrated genomic and transcriptomic analyses while preserving limited archival tissue.
&nbsp;
For FFPE kit selection, bulk pricing on Zymo Quick-DNA/RNA FFPE Miniprep kits, or protocol guidance for aged or low-cellularity archival blocks,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.&nbsp;

FFPE

Quick-DNA/RNA™ FFPE MiniPrep (50 Preps.)

Quick-DNA/RNA FFPE MiniPrep R1009 by Zymo Research: 50-prep kit for simultaneous DNA and RNA isolation from FFPE tissue. No xylene required; 50 µg binding capacity; A260/A280 ≥1.8; total RNA ≥17 nt. Supports RT-PCR, NGS, and microarray. Research use only.

<h2>What are Magnetic Bead DNA/RNA Co-Extraction Kits for Cells, Tissue, and Blood?</h2>
&nbsp;
Magnetic bead DNA/RNA co-extraction kits for cells, tissue, and blood simultaneously isolate genomic DNA and total RNA, including small RNAs &gt;=17 nt from mammalian cell pellets (up to 5 &times; 10^6), solid tissue biopsies (up to 30 mg), and whole blood (up to 50 ul) in 96-well format using paramagnetic silica-coated beads on automation platforms. The Zymo Quick-DNA/RNA MagBead Kit includes DNA/RNA Shield for sample lysis and stabilization, Proteinase K for tissue digestion, and DNase I for on-bead gDNA removal from the RNA fraction, producing both analytes in separate eluates from the same input without any centrifugation during the purification phase. Choose the magnetic bead format for DNA/RNA co-extraction from cells, tissue, and blood when processing 48+ samples per session, when running gene therapy tissue panels across 10+ organ types, or when automation infrastructure eliminates the bottleneck of manual spin-column switching for large sample sets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
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<li>Quick-DNA/RNA&trade; MagBead Kit&nbsp;for simultaneous isolation of total DNA and RNA from cells, tissues, and blood.</li>
<li>Magnetic particle&ndash;based purification for efficient nucleic acid recovery.</li>
<li>High-throughput workflow suitable for processing multiple samples.</li>
<li>Automation-ready protocol for streamlined laboratory workflows.</li>
<li>Purified DNA and RNA are compatible with downstream molecular biology applications.</li>
</ul>
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&nbsp;
<h2>How to Choose a Magnetic Bead DNA/RNA Co-Extraction Kit for Cells, Tissue, and Blood</h2>
&nbsp;
Automation Platform Compatibility
Confirm instrument scripts are available before kit purchase. The Zymo Quick-DNA/RNA MagBead Kit is validated on Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek, and KingFisher platforms. For other instruments, consult MBP's technical team or contact Zymo Research automation support for custom script development timelines and requirements.
DNase I Inclusion and Timing
The Quick-DNA/RNA MagBead Kit includes DNase I for automated on-bead treatment, which integrates into the platform workflow script without additional liquid-handling steps. For spin-column co-extraction kits, DNase I treatment adds a separate 15-minute off-robot incubation step; the bead format eliminates this, reducing total protocol time for RNA-seq-grade RNA in 96-well batches.
Tissue Homogenization Before Bead Extraction
Solid tissue requires homogenization before adding lysate to the bead extraction plate. Tissue homogenization options: (a) Proteinase K digestion in DNA/RNA Shield at 55 degrees C for 30-60 minutes (soft tissues); (b) bead-mill homogenization using a 96-well bead mill with 2.8 mm ceramic beads (fibrous tissues); (c) MagBead Lysis Tubes for direct mechanical lysis in individual tubes before transfer to the 96-well plate. Confirm the homogenization approach is compatible with the platform's liquid transfer steps.
Sample Stabilization With DNA/RNA Shield
DNA/RNA Shield added at tissue collection stabilizes both DNA and RNA at room temperature for up to 30 days, enabling same-day or next-day extraction without cold-chain handling. This is particularly important for gene therapy biodistribution studies where multiple tissue types are collected from the same animal at necropsy and must maintain RNA integrity through shipping or batch processing delays.
Small RNA Retention
Silica-bead binding in the Quick-DNA/RNA MagBead Kit retains the full RNA size spectrum, including miRNAs &gt;=17 nt. For cancer multi-omics studies requiring paired DNA sequencing, RNA-seq, and small RNA-seq from the same tumour biopsy, this single kit delivers all three analyte types from one input without additional small RNA-specific extraction steps.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Zymo Quick-DNA/RNA MagBead Kit yields high-quality gDNA and total RNA (including small RNAs &gt;=17 nt) from any biological sample, with both analytes ready for NGS, RT-qPCR, and microarray. A 2024 study validated simultaneous DNA and RNA extraction for AAV gene therapy tissue panels - adrenal gland, brain, gonads, heart, kidney, liver, lung, skeletal muscle, spinal cord, spleen- using automated co-extraction on a Beckman Coulter system, achieving a 100% pass rate for both VCN analysis (DNA) and transgene expression analysis (RNA) across 10 tissue types. Magnetic bead DNA/RNA co-extraction from tissue is standard in gene therapy pharmacology and toxicology studies, with the Zymo Quick-DNA/RNA MagBead Kit among the most cited formats in published methods papers and regulatory submission CMC sections for AAV gene therapies.
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc.</a> today and get reliable mag bead systems for automated lab workflows.

Quick-DNA/RNA™ MagBead

Quick-DNA/RNA MagBead by Zymo Research — magnetic bead co-purification of genomic DNA (≥40 kb) and total RNA (including microRNA ≥17 nt) from cells, tissue, FFPE, blood, and environmental samples. Binding capacity 15 µg/30 µl beads; ≥50 µl elution; A260/A280 &gt;1.8. Available as R2130 (96 preps) and R2131 (4×96 preps).

Recommended Materials

Sample Preservation

Size Limits

Storage

Quick-DNA/RNA™ MagBead (96 Preps.)

Quick-DNA/RNA™ MagBead (4 x 96 Preps.)

Quick-DNA/RNA™ Microprep Plus Kit

The Quick-DNA/RNA Microprep Plus Kit by Zymo Research is a co-purification kit that simultaneously isolates genomic DNA (≥40 kb, ≤5 µg) and total RNA (≥17 nt, ≤10 µg) from the same sample — cells, tissue, blood, FFPE, and more — in a single workflow. Available in 50-prep (D7005) and 10-prep (D7005T) formats.

Quick-DNA/RNA™ Microprep Plus Kit (50 Preps.)

Quick-DNA/RNA™ Microprep Plus Kit (10 Preps.)

Quick-DNA/RNA™ Miniprep

Quick-DNA/RNA™ Miniprep Kit (D7001) by Zymo Research — 50 preps, simultaneous DNA and RNA purification in &lt;15 min. Up to 100 µg DNA / 50 µg RNA per prep; A260/A280 &gt;1.8; DNA ≥40 kb, RNA ≥17 nt. Lysis Buffer and DNA Prep Buffer also sold separately.

Quick-DNA/RNA™ Miniprep (50 Preps.)

DNA/RNA Lysis Buffer (200 ml)

DNA/RNA Lysis Buffer (50 ml)

DNA Prep Buffer (10 ml)

DNA Prep Buffer (25 ml)

<h2>What are Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column environmental/microbial DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA - including small RNAs 17-200 nt - from feces, soil, water, biofilm, swabs, plant material, and body fluids using bead-beating mechanical disruption with BashingBeads (0.1 mm + 0.5 mm) for unbiased lysis of all microbial cell types, followed by sequential Zymo-Spin column purification in a standard microcentrifuge. The ZymoBIOMICS DNA/RNA Miniprep Kit delivers DNA and RNA in separate fractions - the primary advantage over the combined total nucleic acid eluate of the MagBead format - enabling parallel metagenomic and metatranscriptomic library preparation from one undivided environmental input without post-extraction enzymatic separation steps. Choose the spin-column format when processing 1-24 environmental samples per run, when separate DNA and RNA fractions are required from the outset, or when automation infrastructure is unavailable.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Water Kit to isolate high-purity nucleic acids from water samples using specialized column purification
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Pathogen MiniPrep Kit for rapid co-extraction of pathogen genomes utilizing robust spin-column technology
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS&trade; DNA/RNA Mini Kit to ensure unbiased microbiome profile recoveries using an innovative lysis system&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Separate vs. Combined Fractions
The ZymoBIOMICS DNA/RNA Miniprep natively delivers separate DNA and RNA by column switching: gDNA binds the IIIC column while RNA flows through to the IC column. No post-extraction enzymatic treatment is needed to separate the analytes. This is the critical differentiator from the MagBead format, which requires DNase I treatment of the combined eluate to prepare an RNA fraction for RNA-seq.
PCR Inhibitor Removal Validation
Always measure A260/A230 on both eluates after extraction. Values below 1.5 indicate inhibitor carryover from humic acids (soil) or bile salts (feces) that will suppress PCR amplification and NGS library preparation. If A260/A230 &lt; 1.5, reduce input mass to 50-100 mg and add an optional pass through Zymo-Spin III-HRC Inhibitor Removal Columns on the RNA eluate before downstream use.
Bead-Beating Instrument
A vortex with a horizontal adapter (Vortex-Genie 2 at max speed x 5 min) validates for standard fecal and soil samples. A dedicated bead mill (Precellys, FastPrep-24) at 6,000-6,500 rpm for 40-60 seconds per cycle provides more consistent lysis for tough-walled Gram-positive organisms in high-diversity environmental samples; use 2-3 cycles with 30-second cooling intervals on ice to prevent RNA degradation from heat.
DNA/RNA Shield for Sample Stabilization
DNA/RNA Shield preserves microbial community RNA at room temperature for 30 days. Add Shield directly to the sample tube immediately after collection - before any transport or refrigeration delay - to capture the metabolic RNA expression state at the moment of collection. Shield-stabilized samples load directly into BashingBead Lysis Tubes without additional preparation.
Input Scaling for Low-Biomass vs. High-Biomass Samples
Standard input for feces: 250 mg. Standard input for topsoil: 200 mg. For low-biomass environmental samples (filtered water, air, oligotrophic deep soil): collect maximum biomass by filtering the largest feasible volume and add carrier RNA (5 ng per extraction) to the lysis step to improve column binding of trace nucleic acids. For high-biomass samples (feces, compost), reduce input to 50-100 mg to avoid column overloading and inhibitor carryover.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for the ZymoBIOMICS DNA/RNA Miniprep Kit: DNA yield 2-15 ug from 250 mg feces (A260/A280 &gt;= 1.8, fragment size &gt;= 15 kb for standard input); RNA yield 2-10 ug from 250 mg feces (A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, small RNAs 17-200 nt included). The kit uses Zymo-Spin column technology with OneStep PCR Inhibitor Removal Technology validated for feces, soil, plant material, water, biofilm, swabs, saliva, and body fluids, producing inhibitor-free nucleic acids ready for RT-PCR, arrays, sequencing, and microbiome analyses. The ZymoBIOMICS DNA/RNA Miniprep is widely used in gut microbiome and soil metatranscriptomics research at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
&nbsp;
For bulk pricing on ZymoBIOMICS DNA/RNA Miniprep kits, bead-beating instrument recommendations, or protocol guidance for low-biomass or high-inhibitor environmental samples,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Quick-DNA/RNA™ Pathogen MiniPrep Kit

The Quick-DNA/RNA&trade; Pathogen MiniPrep Kit (R1043) by Zymo Research is a 200-preparation spin-column kit for simultaneous isolation of DNA and RNA from vector-borne pathogens including viruses, bacteria, and protozoa. Compatible vectors include mosquitoes (&le;50 per prep), ticks (1 engorged or &le;5 flat adults or &le;20 nymphs), fleas (&le;10), and deer flies (1 adult), as well as mammalian and avian tissue (&le;10 mg).
Binding capacity: 50 &micro;g per column; size range: 50 nt to ~200 kb; elution: &ge;25 &micro;L (standard 50 &micro;L). All components store at room temperature. The 50-prep version is R1042.
MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Quick-DNA/RNA™ Pathogen MiniPrep (200 Preps.)

Quick-DNA/RNA™ Pathogen MiniPrep (50 Preps.)

<h2>What are Spin-Column Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column viral DNA/RNA co-extraction kits purify viral DNA and RNA simultaneously from plasma, serum, urine, nasopharyngeal swabs, saliva, CSF, and cell culture media using a single-buffer lysis that disrupts viral particles and conditions nucleic acids to bind a Zymo-Spin silica-membrane column in a standard microcentrifuge. Both DNA and RNA elute together in 30-100 ul of DNase/RNase-free water, ready for multiplex RT-qPCR targeting RNA viruses (SARS-CoV-2, influenza A/B, RSV, HIV) and DNA viruses (adenovirus, herpesvirus, HPV, parvovirus) from the same eluate in a single assay. Choose the spin-column viral DNA/RNA format when processing fewer than 50 samples per run, when only a microcentrifuge is available, or when the fastest possible turnaround from sample to detectable eluate is required for point-of-care adjacent testing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-DNA/RNA&trade; Viral Kit (D7020): Efficient spin-column technology designed for the fast co-purification of high-quality viral RNA and viral DNA from liquid specimens.
</li>
<li dir="ltr" aria-level="1">
Virus RNA Kit (R6620-00): Specialized silica-membrane columns created for sensitive extraction of viral RNA, guaranteeing pure yields for subsequent detection processes.
</li>
<li dir="ltr" aria-level="1">
MV Total RNA Isolation (R6313-01): Custom extraction columns designed for the retrieval of total RNA from difficult molecular biology samples, ensuring reliable recovery.
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Single-Buffer vs. Multi-Buffer Lysis
Zymo Quick-DNA/RNA Viral Kit uses a single-step lysis buffer -- no protease incubation, no carrier RNA addition, no ethanol mixing before column loading. This reduces protocol complexity and per-batch error rate. Multi-buffer kits (QIAGEN QIAamp, Thermo PureLink) require preparation of lysis buffer + carrier RNA + ethanol before loading; they are comparable in yield but add setup time per batch.
Input Volume Flexibility
Standard spin-column viral kits accept 50-200 ul of cell-free sample. For low-titer samples requiring larger input volumes to increase sensitivity, check the kit for extended-input protocols; some kits allow 400-1,000 ul plasma with vacuum manifold loading. For samples in the DNA/RNA Shield, direct loading is validated without dilution.
DNA+RNA vs. RNA-Only Recovery
Confirm you need co-extraction before ordering. If your panel includes only RNA viral targets (influenza, RSV, SARS-CoV-2), an RNA-only viral spin-column kit produces equivalent sensitivity with a simpler protocol. Co-extraction is essential when your panel includes HPV, adenovirus, CMV, EBV, parvovirus, or any other DNA virus target - RNA-only kits will under-recover or miss these targets.
Elution Volume and Downstream Assay
Elute in 30-50 ul for RT-qPCR sensitivity; the higher nucleic acid concentration produces Ct improvement of 0.5-1.5 cycles compared to 100 ul elution. For multiplex NGS library preparation, elute in 100 ul and quantify by Qubit dsDNA BR and RNA HS assays separately before library input normalization.
Throughput Scaling
When daily volumes exceed 24 samples, the spin-column format creates centrifuge bottlenecks. At &gt;50 samples per run, transition to the Quick-DNA/RNA Viral 96-well plate format (vacuum manifold) or Quick-DNA/RNA Viral MagBead format (automation) for higher throughput.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column viral DNA/RNA co-extraction: &gt;=70% recovery of RNA and DNA spike-in controls, Ct within 2 cycles of reference extraction for validated viral targets, and A260/A280 &gt;= 1.7 in eluates. The Zymo Quick-DNA/RNA Viral Kit uses a single buffer system validated for enteroviruses, rhinoviruses, coronaviruses (SARS-CoV-2), HIV, HCV, influenza A, flaviviruses (dengue, Zika), measles, parainfluenza, and parvovirus; the kit is used in Zymo's SARS-CoV-2 extraction workflow and has been adopted in published multiplex respiratory panel methods. Spin-column viral co-extraction kits are the standard format in clinical research labs processing 10-50 patient samples per run for research-use multiplex RT-qPCR panels at institutions including Vanderbilt University and MD Anderson Cancer Center.
&nbsp;
For kit recommendations, bulk pricing on Quick-DNA/RNA Viral spin-column kits, or guidance on pairing with multiplex RT-qPCR panels,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.

Spin-Column

MSDS - D7020-1

Quick-DNA/RNA™ Viral Kit

Zymo Research Quick-DNA/RNA™ Viral Kit: co-isolates viral DNA and RNA from ≤400 µL plasma/serum/urine/blood/swab in ~5–10 min; includes DNA/RNA Shield 2× for room-temp sample inactivation. D7020 (50 preps), D7021 (200 preps); Viral DNA/RNA Buffer (D7020-1-25/100 mL).