Zymo Consumables

Devices & Instruments

Protocol

EZ-Vac Vacuum Manifold

EZ-Vac Vacuum Manifold (S7000) by Zymo Research — processes 1–20 Zymo-Spin columns simultaneously under vacuum (&ge;400 mm Hg). Linear polyethylene body, one-way Luer-Lock stopcocks, polypropylene vacuum connector. Compatible with most Zymo nucleic acid purification kits.

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EZ-Vac96 Vacuum Manifold

EZ-Vac96™ Vacuum Manifold S7003 by Zymo Research — processes up to 96 samples simultaneously using vacuum-driven column flow, eliminating centrifugation. Compatible with ZymoPURE™ 96 Plasmid Miniprep Kit (D4214, D4215); requires vacuum pump capable of up to 300 mmHg and 6.5 mm ID tubing. Chemical-resistant construction with external catch flask. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Required Equipment

Columns, Plastics & Accessories

Elbow Hose Connector (1 Pack)

Elbow Hose Connector S7004 by Zymo Research is a polypropylene 90-degree connector (3/8&quot; hose barb × 3/8&quot; male pipe thread) for connecting vacuum tubing to the EZ-Vac™ Vacuum Manifold (S7000). Supplied as a single connector.

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Plates

Elution Plate (2 Plates)

C2003 — Elution Plate (2 Plates): 96-well V-bottom collection plates with 90 µl per well capacity, for use with Zymo Research spin plate purification systems to collect purified DNA or RNA eluates during high-throughput nucleic acid purification workflows.

Spin Columns

EndoZero II Spin Columns (25 Pack)

C1060-25 by Zymo Research is a 25-pack of EndoZero™ II Spin Columns for polishing plasmid DNA purified with ZymoPURE II Midiprep, Maxiprep, or Gigaprep kits to ≤0.025 EU/µg endotoxin — 40× lower than standard transfection-grade. Accepts up to 400 µg plasmid input; 2-minute incubation + centrifugation.

EndoZero Spin Columns

C1051-10 by Zymo Research is a 10-pack of EndoZero Spin Columns engineered for use with the ZymoPURE II Maxi and ZymoPURE II Gigaprep Plasmid Purification Kits to produce endotoxin-free plasmid DNA from E. coli. The resin selectively removes LPS without co-purifying plasmid DNA, enabling the fastest available endotoxin-free Maxi/Gigaprep workflow. Available from MBP, a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many other institutes across the USA.

EndoZero Spin Columns (10 Pack)

EndoZero Spin Columns (5 Pack)

Syringe-Filters

Midi Filter (25 Pack)

C1034-25 by Zymo Research is a 25-pack of 15 mL polypropylene Midi Filters for removing cellular debris from homogenized samples prior to DNA or RNA purification on Zymo-Spin V-E Columns (C1024-25). Compatible with centrifuge and vacuum manifold processing.

Neoprene Stopper (1 Pack)

Neoprene Stopper (S7002) by Zymo Research — replacement sealing component for the EZ-Vac Vacuum Manifold (Cat# S7000). Sold as 1 Pack.

One-Way Luer-Lock Clear Stopcock (20 Pack)

The One-Way Luer-Lock Clear Stopcock by Zymo Research (S7001) is a 20-pack of transparent, single-use stopcocks with luer-lock fittings for precise vacuum flow control on the Zymo Research EZ-Vac Vacuum Manifold. Each stopcock delivers individual column control during vacuum-assisted DNA, RNA, and protein purification workflows.

PCR Strip MagStand

3DP-1002 by Zymo Research is the PCR Strip MagStand, a magnetic bead separation stand for standard 0.2 mL PCR tubes and strip tubes. Made in the USA; validated for Zymo-Seq RiboFree Total RNA Library Kit (R3000) SPRI bead cleanup and compatible with general AMPure/SPRI workflows in strip-tube format.

Silicon-A Plate (2 Plates)

Silicon-A Plate C2001 by Zymo Research is a 96-well silica-matrix plate for centrifuge-based high-throughput DNA and RNA purification, with up to 5 µg binding capacity per well, 600 µl well volume, and ≥30 µl elution. Supplied as 2 plates per pack.

DataSheet

Silicon-A™-HRC Plate (2 Plates)

C2009 by Zymo Research is the Silicon-A™-HRC Plate (2 plates, 96-well format), a replacement component for the OneStep-96 PCR Inhibitor Removal Kit (D6035). The Silicon-A™-HRC matrix removes polyphenolics, humic/fulvic acids, tannins, and melanin from DNA and RNA extracts for direct use in PCR, qPCR, or sequencing. Requires Prep Solution before use. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.