Nest Scientific Consumables

Caps

<h2 data-section-id="1j61jib" data-start="0" data-end="44">What Are Laboratory Caps and Hair Covers?</h2>
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Laboratory caps and hair covers are disposable head protection PPE designed to fully contain hair, scalp, and in many cases ears, to prevent contamination transfer into controlled laboratory, cleanroom, pharmaceutical, and clinical environments. The most common type is the bouffant cap, which provides full coverage using a gathered, elasticated design made from non-woven polypropylene.
Unlike hair nets, which contain open mesh structures, bouffant caps are made from closed, non-woven material that significantly reduces particulate shedding. This makes them the standard requirement in most ISO-classified environments where contamination control is critical.
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<h2 data-path-to-node="1">Whats inside :</h2>
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<ul style="list-style-type: disc;">
<li dir="ltr" role="presentation">High-quality PP non-woven material that provides a superior dustproof barrier and blocks microorganisms effectively.</li>
<li dir="ltr" role="presentation">Secure, stay-put fit that remains comfortable without feeling tight, even during the longest shifts.</li>
<li dir="ltr" role="presentation">Engineered for maximum airflow to prevent heat buildup while being gentle on the skin to avoid any allergic reactions.</li>
<li dir="ltr" role="presentation">The ideal choice for medical facilities, cleanrooms, and sanitation units where hygiene is the top priority.</li>
</ul>
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<h2 data-section-id="tho3ti" data-start="891" data-end="939">How to Choose Laboratory Caps and Hair Covers</h2>
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Size selection Cap sizes are measured by fully-stretched elastic band diameter: 21 inches covers most personnel with short to medium hair; 24 inches accommodates long, thick, or high-volume hair; 28 inches suits very high-volume hair, dreadlocks, or wrapped hair styles. The 21-inch format is the most widely stocked and cost-effective default for mixed-workforce labs. Over-sizing increases the risk of the cap slipping and exposing hair during a shift; under-sizing causes discomfort and reduces compliance. &nbsp; Material and barrier rating Standard non-woven spunbond PP bouffant caps at 40 gsm are appropriate for ISO 7 (Class 10,000) environments covering the majority of research, academic, clinical, and pharmaceutical lab settings. SMS caps with a meltblown middle layer provide enhanced aerosol and droplet barrier for pharmaceutical cleanrooms and controlled environments requiring higher barrier performance. Confirm the product's documented ISO classification or particle generation test data when sourcing for validated or GMP-regulated environments. &nbsp; Latex-free elastic Quality-tier bouffant caps use latex-free pleated elastic bands that provide uniform tension across different head shapes without latex allergen exposure. Pleated (gathered) elastic distributes pressure evenly and reduces the headband discomfort of non-pleated alternatives during long wear sessions. Confirm latex-free elastic in the product CoA for environments with latex allergy protocols. &nbsp; Case quantity Bouffant caps are typically supplied in 1,000-piece cases (100 per bag, 10 bags) for easy floor stock management. Separate 21-inch and 24-inch SKUs should be stocked in proportion to workforce hair length distribution. For a 15-person lab with daily cap changes, one 1,000-piece case lasts approximately 67 working days. &nbsp; Lot traceability For ISO-certified cleanroom environments, select bouffant caps with product code and lot number traceability printed on the bag and outer carton. This supports documentation requirements for ISO 7 gowning procedures and facility audits.
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<h2 data-section-id="1jl751w" data-start="4561" data-end="4586">Specifications Context</h2>
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Laboratory bouffant caps are typically manufactured from 28&ndash;40 gsm spunbond polypropylene in ISO-certified facilities and packaged in bulk cases for high-volume usage. Most standard laboratory caps are designed to meet ISO 7&ndash;9 cleanroom requirements, while higher-grade SMS and cleanroom-certified variants support stricter controlled environments.
Ready to equip your facility? <a href="https://mbpinc.net/contact-us">Reach out to the MBP team</a> for a personalized quote and keep your workspace protected with confidence.

Blue mushroom cap, PP non-woven, double elastic, 100/pk, 1000/cs

921001 by NEST Scientific is a blue disposable mushroom-style head cover made from breathable PP non-woven fabric with a double elastic band (crown + perimeter) for secure, comfortable fit. Non-sterile, single-use, latex-free. 5-year shelf life. Suitable for pharmaceutical GMP, cleanrooms, food processing, medical facilities, and labs. 100/pk, 1,000/cs.

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
</li>
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Sterile Bottle-Top Filters for secure aseptic transfer
</li>
</ul>
<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Datsheet

Vacuum Filtration Systems  (PES Membrane)

Bottle Top Vacuum Filter, PES, Sterile

NEST sterile PES bottle top vacuum filters in 15 variants — five volumes (50–1000 mL) × three pore sizes (0.1, 0.22, 0.45 µm). E-beam SAL 10⁻⁶, low protein binding, 50 mm membrane. SKUs 347011–344013. 1/pk, 24/cs.

Manufacturer

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PES, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PES, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PES, Sterile, 1/pk, 24/cs

Vacuum Filtration Systems (PVDF Membrane)

Bottle Top Vacuum Filter, PVDF, Sterile

Bottle Top Vacuum Filter, PVDF, Sterile by NEST Scientific are E-beam sterilized to SAL 10⁻⁶, manufactured in an ISO 7 cleanroom, certified DNase/RNase-free and non-pyrogenic, for protein solution filtration, buffer sterilization, and media preparation. Available in 50mL, 150mL, 250mL, 500mL, and 1000mL, each in 0.10µm, 0.22µm, and 0.45µm pore sizes.

50 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

50 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

50 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

150 mL Bottle Top Vacuum Filter, 0.22μm, PVDF, Sterile,1/pk, 24/cs

150 mL Bottle Top Vacuμm Filter, 0.45μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

250 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

250 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

500 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

500 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.1 μm, PVDF, Sterile, 1/pk, 24/cs

1000 mL Bottle Top Vacuum Filter, 0.22 μm, PVDF, Sterile,1/pk, 24/cs

1000 mL Bottle Top Vacuμm Filter, 0.45 μm, PVDF, Sterile, 1/pk, 24/cs

Cryogenic Vials

<h2>What Are Cryogenic Vials?</h2>
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Cryogenic vials are specialized screw-cap laboratory containers manufactured from cryogenic-grade virgin polypropylene (PP) and designed to withstand extreme temperatures ranging from &minus;196&deg;C in liquid nitrogen storage to +121&deg;C during steam autoclaving. They serve as the primary storage vessel for biological materials requiring long-term preservation, including cell lines, serum, plasma, blood samples, DNA, RNA, microbial cultures, and other biospecimens. Common vial capacities include 1.2 mL, 1.5 mL, 1.8 mL, 2 mL, 3 mL, and 5 mL, with the 2 mL format being the most widely used standard for cell culture and biobanking applications. Most cryogenic vials feature leak-resistant screw caps with silicone sealing gaskets and dedicated writing areas for sample identification. For storage in liquid nitrogen, it is important to use vials specifically certified for cryogenic applications, as standard laboratory tubes are not designed to tolerate temperatures as low as &minus;196&deg;C.
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<h2>What You Will Find:</h2>
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<ul>
<li><a href="https://mbpinc.net/consumables/cryogenic-storage/cryogenic-vials/cryo-boxes" title="Cryo Boxes">Cryo Boxes:</a> High-durability polycarbonate storage systems with integrated grids for rapid, indexed sample retrieval in ultra-low environments.</li>
<li><a href="https://mbpinc.net/consumables/cryogenic-storage/cryogenic-vials/freezer-boxes" title="Freezer Boxes">Freezer Boxes:</a> Moisture-resistant and stackable organizers designed to maximize space and maintain thermal consistency within -20 degree celsius and -80 degree celsius freezers.</li>
<li><a href="https://mbpinc.net/consumables/cryogenic-storage/cryogenic-vials/externally-threaded-cryogenic-vials" title="Externally Threaded Cryogenic Vials">Externally Threaded Cryogenic Vials</a>: Designed with threads on the outside of the vial to minimize contamination risk and maximize the internal volume for sample storage.</li>
<li><a href="https://mbpinc.net/consumables/cryogenic-storage/cryogenic-vials/internally-threaded-cryogenic-vials" title="Internally Threaded Cryogenic Vials">Internally Threaded Cryogenic Vials</a>: Featuring a silicone O-ring seal and internal threads to provide the most secure, leak-proof closure for vapor-phase LN2 storage.</li>
</ul>
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<h2>How to Choose Cryogenic Vials</h2>
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Thread Type: Internal vs. External
Thread design influences both contamination control and workflow compatibility. Internal-thread vials position the sealing mechanism inside the neck of the vial, reducing the external surface area exposed to contaminants and making them a common choice for cell culture and biosafety-sensitive applications. External-thread vials place the threads on the outside of the vial neck, maximizing internal storage volume and improving compatibility with automated capping, decapping, and robotic handling systems. Laboratories using automated biobanking workflows often prefer external-thread formats.
Volume Selection
Vial capacity should match the intended sample type and storage requirements. The 1.8&ndash;2.0 mL size is commonly used for cell lines, serum aliquots, and general biospecimen storage because it fits standard cryogenic storage boxes. Smaller 1.2 mL formats support high-density storage of limited-volume samples, while larger 3&ndash;5 mL vials are suitable for tissue specimens, bacterial glycerol stocks, and applications requiring larger sample volumes.
Sterility and Purity Certification
For cell culture, molecular biology, and clinical research applications, cryogenic vials should be supplied with certifications confirming they are RNase-free, DNase-free, non-pyrogenic, and endotoxin-free. Many laboratories also prefer gamma-sterilized vials, which reduce contamination risk and eliminate the need for additional sterilization before use. Traceable lot documentation and certificates of conformity are often required in regulated environments.
Self-Standing vs. Round Bottom Designs
Self-standing cryogenic vials feature a molded base that allows them to remain upright on laboratory benches without additional support, reducing the risk of spills during sample handling. Round-bottom designs are compatible with a broader range of storage racks and cryogenic systems but generally require a rack or holder during bench-top use. The choice largely depends on laboratory workflow and storage equipment.
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<h2>Specifications Context</h2>
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Cryogenic vials are typically manufactured from high-purity polypropylene because of its excellent durability and resistance to ultra-low temperatures. Products intended for liquid nitrogen immersion should carry explicit certification for liquid-phase storage, as some vials are approved only for vapor-phase applications. Vial dimensions, particularly outer diameter, determine compatibility with freezer boxes, racks, and automated storage systems, so measurements should be confirmed before mixing products from different manufacturers. Modern biobanking facilities increasingly use externally threaded, 2D-barcoded cryogenic vials integrated with laboratory information management systems (LIMS) to improve inventory tracking, sample traceability, and large-scale repository management.
Protect your research legacy. <a href="https://mbpinc.net/contact-us">Connect with the Molecular Biology Products Inc. team</a> for a custom quote on our complete cryogenic storage range.

Cap Insert for Cryogenic Vials

NEST polypropylene cap inserts for external-thread cryogenic vials in six colors: White (611201), Red (611202), Yellow (611203), Blue (611204), Green (611205), Purple (611206). Color-coded secondary ID and thread protection. 100/pk, 1,000/case.

Cap Insert for Cryogenic Vial, White, New Model, 100/pk, 1000/cs

Cap Insert for Cryogenic Vial, Red, New Model,100/pk, 1000/cs

Cap Insert for Cryogenic Vial, Yellow, New Model,100/pk, 1000/cs

Cap Insert for Cryogenic Vial, Blue, New Model,100/pk, 1000/cs

Cap Insert for Cryogenic Vial, Green, New Model, 100/pk, 1000/cs

Cap Insert for Cryogenic Vial, Purple, New Model,100/pk, 1000/cs

Carboys

Carboy, Autoclavable, with Handle, Non-Sterile

NEST autoclavable USP Grade VI PP carboys with handle, rated −40°C to 121°C. Four variants: 10 L without tap (789011), 10 L with tap (789001), 20 L without tap (789111), 20 L with tap (789101). ISO 13485 certified, lot-tested. 4–6/cs.

10L Carboy, Autoclavable, with Handle ,without Tap, Non-Sterile, 6/cs

20L Carboy, Autoclavable, with Handle ,without Tap, Non-Sterile, 3/cs

10 L Carboy, Autoclavable, with Handle, with Tap, non-sterile, 4/cs

20 L Carboy, Autoclavable, with Handle, with Tap, non-sterile, 3/cs

Cell Culture Dishes

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
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Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

DataSheet

Product Literature

Cell Culture Dish Specifications

Cell Culture Dish, TC Treated, Sterile

TC-Treated Cell Culture Dish by NEST Scientific — 35 mm TC-treated polystyrene cell culture dish, 9 cm&sup2; growth area, 1.8&ndash;2.7 mL working volume, vented lid, sterile, with numeric position indicators. 20/pk, 500/cs. Available in 35 mm (706001), 60 mm (705001), 100 mm (704004, 704001), and 150 mm (715001).

35mm Cell Culture Dish, TC, sterile 20/pk, 500/cs

60mm Cell Culture Dish, TC, sterile 20/bag, 500/cs

100mm Cell Culture Dish, TC, sterile 5/bag, 300/cs

100mm Cell Culture Dish, TC, sterile 20/bag, 300/cs

150mm Cell Culture Dish, TC, sterile 5/pk,100/cs

Cell Culture Dish, with Gripping Ring Specifications

Cell Culture Dish, with Gripping Ring, TC Treated, Sterile

Cell Culture Dish with Gripping Ring by NEST Scientific — TC-treated USP Grade VI polystyrene, plasma-etched, E-beam sterilized to SAL 10⁻⁶; pyrogen-free, cytotoxin-free, DNase/RNase-free, and BSE/TSE-free. ISO 7 cleanroom manufactured. Available in 35 mm (706201), 60 mm (705201), and 100 mm (704201).

35mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

60mm Cell Culture Dish, with Gripping Ring, TC, Sterile, 20/pk, 500/cs

100mm Cell Culture Dish, with Gripping Ring, TC, Sterile 20/bag, 300/cs

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
</li>
<li dir="ltr" aria-level="1">
Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
</li>
<li dir="ltr" aria-level="1">
Aseptic Security with certified RNase-, DNase-, and Pyrogen-free to maintain the highest purity standards.
</li>
<li dir="ltr" aria-level="1">
Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
</li>
</ul>
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

Cell Culture Flask, Plug Seal Cap, Non-Treated Specifications

Cell Culture Flask, Plug Seal Cap, Non-treated, Sterile - Nest Scientific

Cell Culture Flask, Plug Seal Cap, Non-Treated by NEST Scientific — 25 cm² T-25 cell culture flask with plug seal cap and non-treated surface. 50 mL total volume, sterile, 10/pk. For suspension cells in CO₂-independent media or transport applications. Available in 25 cm² (707011), 75 cm² (708011), 175 cm² (709011), and 225 cm² (721011).

25cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Plug Seal Cap, Non-Treated, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
<li dir="ltr" aria-level="1">
Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
</li>
<li dir="ltr" aria-level="1">
Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
</li>
<li dir="ltr" aria-level="1">
Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
</li>
<li dir="ltr" aria-level="1">
Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
</li>
<li dir="ltr" aria-level="1">
Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

Cell Culture Flask, Plug Seal Cap, Treated Specifications

Cell Culture Flask, Plug Seal Cap, TC, Sterile - Nest Scientific

The NEST Scientific 707001 Series are TC-treated cell culture flasks with an HDPE plug seal cap, manufactured from USP Class VI virgin polystyrene. E-beam sterilized to SAL 10⁻⁶, DNase/RNase-free, cGMP compliant (ISO 13485:2016, ISO 9001, ISO 7 cleanroom). Working volume 5–10 mL; maximum 30 mL. Ships 10/bag, 200/case. Vent-cap variant: 707003. Available in four growth surface sizes: 25 cm² T-25 (707001), 75 cm² T-75 (708001), 175 cm² T-175 (709001), and 225 cm² T-225 (721001) — all TC-treated, plug seal cap, sterile.

25cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Plug Seal Cap, TC, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Flask, Vent Cap, Non-Treated Specifications

Cell Culture Flask, Vent Cap, Non-Treated, Sterile - Nest Scientific

Cell Culture Flasks, Vent Cap, Non-Treated by NEST Scientific — 25 cm² T-25 cell culture flask with vent cap and non-treated surface. 50 mL total volume, sterile, 10/pk. Designed for suspension cells, hybridoma culture, and applications where surface adhesion must be prevented. Available in four sizes: 707013 (25cm²), 708013 (75cm²), 709013 (175cm²), 721013 (225cm²).

25cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Vent Cap, Non-Treated, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Flask, Vent Cap, TC Treated Specifications

Cell Culture Flask, Vent Cap, TC, Sterile - Nest Scientific

NEST Scientific Cell Culture Flasks with Vent Cap are TC-treated flasks made from USP Class VI polystyrene and e-beam sterilized to SAL 10⁻⁶. ISO 13485:2016 and cGMP certified. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>. Available in four sizes: 25, 75, 175, and 225 cm² — all TC-treated, vent cap, sterile.

25cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 50mL, 10/pk, 200/cs

75cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 250mL, 5/pk, 100/cs

175cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 750mL, 5/pk, 40/cs

225cm2 Cell Culture Flask, Vent Cap, TC, sterile, Maximum Working Volume 950mL, 5/pk, 25/cs

Cell Culture Plates

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
&nbsp;
<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

<h2 class="p2">What are TC-Treated Cell Culture Plates?</h2>
 TC-treated (tissue culture&ndash;treated) cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces have been modified by vacuum plasma treatment to introduce negatively charged oxygen-containing groups (carboxyl, carbonyl, hydroxyl), reducing the water contact angle from ~75&ndash;90&deg; (untreated PS) to below 10&deg; (hydrophilic). This surface modification directly enables attachment, spreading, and monolayer formation of adherent mammalian cell lines. TC-treated plates are the default format for all adherent mammalian cultures, cell-based assays, ELISA preparation, compound cytotoxicity screening, transfection, and gene expression studies.&nbsp; 
<h2 class="p1">What you will find:</h2>
&nbsp;
&bull; 6, 12, 24, 48, and 96 well formats for different workflows &bull; Tissue-culture-treated surfaces for reliable cell attachment &bull; Clear plates for easy monitoring under the microscope &bull; Uniform wells for consistent seeding and growth &bull; Sterile options to maintain clean conditions &bull; Lids designed to reduce contamination and evaporation
&nbsp;
<h2 class="p2">How to Choose TC-Treated Cell Culture Plates</h2>
 Well Format by Cell Number and Assay Type 6-well TC-treated plates (9.4 cm&sup2;/well) are the standard for Western blot preparation, siRNA transfection, viral infection, protein overexpression, and colony-forming unit (CFU) assays requiring &ge;3 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) suit medium-scale reporter assays, luciferase quantification, and ELISA from cell lysate. 24-well plates (1.9 cm&sup2;/well) are versatile for cytotoxicity dose-response in 4&ndash;6 conditions per plate, primary cell experiments, and viral plaque assays. 96-well flat-bottom TC-treated plates (0.32 cm&sup2;/well) are the universal format for cell viability (MTS, MTT, CCK-8), compound screening, ELISA, reporter gene assays, and qPCR lysate preparation across 8&ndash;12 concentrations with triplicate wells. 384-well plates reduce reagent cost per compound for HTS drug discovery programs. Lot-to-Lot TC Treatment Consistency For longitudinal drug screening, biomarker validation, or multi-site studies, lot-to-lot variation in TC treatment can alter cell attachment density, proliferation rate, and assay Z-factor. NEST Scientific verifies vacuum plasma treatment by water contact angle goniometry on each manufacturing lot; the release criterion is contact angle &lt;10&deg;. Request lot certificates when adopting TC-treated plates for GLP- or GMP-regulated assay workflows. Low-Evaporation EDGE Plates For 96-well dose-response assays running 72&ndash;96 hours, peripheral (edge) well evaporation can increase medium osmolality in the outer ring by 10&ndash;20% versus inner wells, introducing a systematic bias in IC50 determinations. NEST Low Evaporation EDGE 96-well TC-treated plates use an internal well ridge design that reduces edge-well evaporation during extended incubations. Use EDGE plates for any dose-response study where incubation time exceeds 48 hours. Cell Line Compatibility TC-treated NEST plates support attachment and monolayer culture of: HeLa (cervical), HEK293 (adherent), A549 (lung), MCF-7 (breast), U2OS (bone), CHO-K1 (ovary, adherent), Vero (kidney), MDCK (canine kidney), NIH 3T3 (mouse fibroblast), MRC-5, 2BS, 293T, L-929, and standard primary cultures including dermal fibroblasts, endothelial cells, and epithelial cells. Do NOT use TC-treated plates for Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9, Hi5, or any non-adherent cell line. Plate Reader and Automation Compatibility NEST Scientific TC-treated 96-well flat plates are compatible with all common microplate readers (BioTek Synergy H1, Molecular Devices SpectraMax i3x, PerkinElmer EnVision, BMG Labtech CLARIOstar) for absorbance, fluorescence intensity, and luminescence detection. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS dimensions for automated liquid handlers (Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek i7). 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific TC-treated cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry; the lot release criterion: contact angle &lt;10&deg;. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS footprint. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free, non-pyrogenic per lot certificate. Clear polystyrene lids included; individually wrapped in peelable film. Alphanumeric well coordinates on all &ge;12-well formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, NEST Scientific TC-treated plates across all core well formats (6- through 384-well) are stocked by MBP. 
Use treated plates when attachment drives your results. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to get reliable plates for keeping your cells anchored, healthy, and consistent across every well.&nbsp;

Treated Cell Culture Plates

Cell Culture Plates - Bulk Packaging

Cell Culture Plates - Bulk Packaging by NEST Scientific are TC-treated flat-bottom plates in bulk packaging (10/bag). USP Class VI polystyrene, E-beam sterilized to SAL 10⁻⁶, pyrogen/DNase/RNase/BSE/TSE tested, ISO 7 cleanroom manufactured, SBS/ANSI footprint. Available in 6-well (703002), 12-well (712002), 24-well (702002), 48-well (748002), and 96-well (701002). Available through MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.