Erlenmeyer Flasks

Erlenmeyer Flasks Accessories

<h2 dir="ltr">&nbsp;What are Erlenmeyer Flask Accessories?</h2>
 Erlenmeyer flask accessories are the replacement caps, transfer cap assemblies, vent filters, and adapter fittings used alongside PC and PETG shake flasks to customize gas exchange, enable closed-system liquid transfer, and replace consumable caps between culture runs. Accessory selection determines whether a shake flask acts as a standard culture vessel, a sealed storage container, or a closed-system liquid transfer assembly in a cGMP workflow.
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<h2 dir="ltr">What you will find:</h2>
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<li>Accessories compatible with high-efficiency Erlenmeyer flasks in 2 L, 3 L, and 5 L formats.</li>
<li>Available in bi-directional, inverted, and multifunctional transfer cap configurations.</li>
<li>Supports both standard-neck and wide-mouth 3 L flask designs.</li>
<li>Includes options with integrated silicon tubing for transfer applications.</li>
<li>Enables customization of flask workflows through interchangeable transfer cap accessories.</li>
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<h2 dir="ltr">How to Choose Erlenmeyer Flask Accessories</h2>
 Vented Caps: Standard vs. Oversized Membrane Standard vented Erlenmeyer caps have a 0.22 &micro;m PTFE membrane in a small-diameter HDPE housing. NEST Scientific vented caps feature an oversized membrane area that increases the gas exchange surface, supporting higher oxygen mass transfer for dense suspension cultures at elevated shaker speeds. At 150 RPM with 20&ndash;25% fill, NEST oversized vented caps provide sufficient oxygen transfer for mammalian suspension cultures at densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting densities above 10 &times; 10⁶ cells/mL in a standard shake flask format, a sparged bioreactor system is more appropriate. Plug Seal Caps: Autoclave vs. Non-Autoclave HDPE plug seal caps for NEST PC Erlenmeyer flasks are compatible with autoclave cycles at 121 &deg;C when loosened 1&ndash;1.5 turns before autoclaving to prevent steam pressure buildup. Plug seal caps for NEST PETG flasks are compatible with static incubation and transport but not autoclaving (PETG maximum temperature: +60 &deg;C). Confirm whether your accessory cap is rated for autoclave use before including it in a steam sterilization cycle. Transfer Cap Assemblies for Closed-System Culture NEST customized transfer cap assemblies combine an Erlenmeyer-compatible cap body with a bi-directional transfer port, TPE tubing, and a Luer lock or CPC connector &mdash; enabling aseptic inoculation, sampling, or media addition to a running shake flask culture without opening the flask. This closed-system capability is used in cGMP process development, GMP seed train management, and contamination-critical research workflows. Transfer caps are available in sizes matching NEST 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, and 3,000 mL flask neck formats. Cap Sizing and Flask Compatibility Erlenmeyer flask caps are sized to the neck thread diameter of the flask. NEST Scientific PC and PETG Erlenmeyer flasks use proprietary screw-thread neck dimensions &mdash; confirm the specific cap catalog number matches your flask's neck size before ordering replacement caps. Do not mix cap brands across flask brands without verifying thread compatibility; mismatched caps create gas leaks at the vent membrane, contaminating cultures. Replacement Cap Frequency for Reusable PC Flasks For reusable PC flasks, replace the vented cap after each culture run to prevent membrane clogging from media splatter, condensation, and biofilm formation on the PTFE membrane. Reusing a contaminated or clogged vent membrane reduces gas exchange efficiency, creating a DO deficit that depresses cell growth without a visible contamination event. NEST vented caps for PC flasks are available in bulk packs for cost-efficient per-run replacement. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific Erlenmeyer flask accessories &mdash; including replacement vented caps, plug seal caps, and transfer cap assemblies &mdash; are manufactured from USP Class VI HDPE (cap housing) and USP Class VI PTFE (0.22 &micro;m membrane), with TPE tubing in transfer assemblies. All accessory caps are sterile and non-pyrogenic per the lot certificate. The 0.22 &micro;m membrane pore size is the standard for microbial retention in cell culture venting applications. Transfer cap assemblies compatible with the NEST Erlenmeyer flask line are available in sizes for 125 mL through 3,000 mL flasks, individually wrapped in sterile packaging. NEST Scientific is ISO 9001:2015 and ISO 13485 certified.&nbsp; 
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Erlenmeyer Flask Accessories

DataSheet

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap

The 5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap by NEST Scientific is a sterile single-use transfer cap (cap only, 1/pk, 4/cs) in three configurations: C10411-AJE092B (TPE 92 cm 1/8&#34; ID, Luer lock sampling nozzle, 0.22 &#956;m &#934;50 mm), C10411-DZB090B (TPE 90 cm 1/4&#34; ID, 0.22 &#956;m &#934;24 mm), and C10411-GBB100B (silicone 100 cm 3/16&#34; ID, male CPC, 0.22 &#956;m &#934;24 mm); E-beam SAL 10&#8315;&#8706;, ISO 13485.

Manufacturer

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube (92cm 1/8" ID 1/4" OD) and a Sampling Nozzle, Vent Filter (0.22 μm Φ50mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube ( 90cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with Silicon Tube (100cm 3/16" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

Product Literature

Bi-directional Transfer Cap with Silicon Tube

Bi-directional Transfer Cap with Silicon Tube by NEST Scientific — bi-directional transfer cap for 125 mL Erlenmeyer flasks with 50 cm silicone tube (1/8&quot; ID &times; 1/4&quot; OD), male CPC connector with female sealing cap, and 0.22 &mu;m (&Phi;42mm) vent filter. E-beam sterile (SAL 10-6), cap only, 1/pk, 4/cs. Available in 125mL (C30821-EAC050B CPC / C30821-EFC050B Luer Lock), 250mL (C30921-EAC050B / C30921-EFC050B), 500mL (C31021-EAC050B / C31021-EFC050B), 1000mL (C31121-EAC050B / C31121-EFC050B).

125 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

125 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

250mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

250 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

500 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

500 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer Cap, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male CPC Connector with Female Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (50cm 1/8" ID 1/4" OD), Male Luer Lock Connector with Luer CapVent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

<h2 dir="ltr">What are Cell Culture Erlenmeyer Flasks?</h2>
 Cell culture Erlenmeyer flasks (also called shake flasks or conical flasks) are single-use, sterile plastic vessels with a conical body, flat base, and narrow neck used for suspension cell culture on orbital shakers and incubator shakers, microbial culture, and media preparation. Unlike rectangular T-flasks designed for static monolayer culture, Erlenmeyer flasks are engineered for shaking: their wide conical base maximizes liquid surface area for oxygen transfer, while the narrow neck minimizes evaporation and contamination risk. Choose PC Erlenmeyer flasks for autoclavable reuse and high-temperature media preparation; choose PETG for single-use suspension culture and processes requiring BPA-free materials.
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<h2 dir="ltr">What you will find:</h2>
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<a href="erlenmeyer-flasks/petg-erlenmeyer-flasks">PETG Erlenmeyer flasks: </a>strong choice for cell culture applications that need excellent clarity, reliable handling, and convenient single-use performance. They are lightweight, durable, and ideal for shaker culture, media preparation, and general suspension cell workflows.
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<a href="erlenmeyer-flasks/pc-erlenmeyer-flasks">PC Erlenmeyer flasks: </a>built for applications that require greater strength and higher impact resistance. They are well-suited for demanding lab environments, including vigorous shaking, transport, and workflows where added durability is important.
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<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/consumables/cell-culture/erlenmeyer-flasks/erlenmeyer-flask-accessories">Erlenmeyer Flask Accessories: </a>Includes bi-directional, inverted, and multifunctional transfer caps, plus silicon tube assemblies for 2 L, 3 L, 3 L wide-mouth, and 5 L high-efficiency flasks, supporting sterile transfer and closed-system workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Erlenmeyer Flasks for Cell Culture</h2>
 PC vs. PETG Material PC (polycarbonate) Erlenmeyer flasks are autoclavable at 121 &deg;C (typically 5&ndash;10 cycles), shatter-proof, crystal-clear, and rated from &minus;135 &deg;C to +135 &deg;C. PC is ideal for labs that sterilize media or wash in-house. PC contains BPA. PETG Erlenmeyer flasks are single-use, BPA-free, rated from &minus;80 &deg;C to +60 &deg;C, and cannot be autoclaved. They provide a contamination-free, cleaning-free alternative to glass and PC for high-throughput suspension culture operations. Flat Base vs. Baffled Base Flat-base Erlenmeyer flasks provide gentler swirling for slow-growth cell lines and media mixing. Baffled-base flasks have three or four angled internal baffles that interrupt the swirling flow, increasing turbulence, raising dissolved oxygen (DO) by up to 2&times; compared to flat-base flasks at the same orbital speed, and reducing cell sedimentation. Baffled flasks are the standard for CHO-S, HEK293-F, Sf9, microbial, and yeast cultures requiring high oxygen transfer rates. NEST Scientific offers baffled and flat-base versions in both PC and PETG across all core volumes. Vented vs. Plug Seal Cap Vented caps contain a 0.22 &micro;m hydrophobic PTFE membrane that allows free O₂/CO₂ gas exchange while excluding microbial contamination. The NEST vented cap features an oversized vent area that improves gas exchange beyond standard-size membrane caps, supporting oxygen-demanding suspension cultures at higher cell densities. Plug seal caps create a fully sealed environment for media storage, static incubation, or transport outside the incubator. Always use vented caps for actively growing suspension cultures on an orbital shaker. Volume and Fill Ratio Erlenmeyer flasks should be filled to no more than 20&ndash;25% of the nominal flask volume for suspension shaker culture to maintain adequate headspace for oxygen transfer. A 500 mL flask running at 20% fill holds 100 mL of culture; a 1,000 mL flask holds 200 mL. Overfilling reduces oxygen transfer efficiency and creates foam that blocks the vented cap membrane. For media storage (no shaking), the fill volume can approach 80% of nominal. Shaker Compatibility NEST Scientific Erlenmeyer flasks (125 mL&ndash;3,000 mL) are compatible with standard laboratory orbital shakers, including those from Infors HT, New Brunswick Scientific (Innova), and Thermo Scientific MaxQ. Confirm flask neck O.D. and base diameter against your shaker's flask holder clips or clamp system before ordering large quantities. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade polycarbonate, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PETG Erlenmeyer flasks are E-beam sterilized and certified DNase/RNase-free, pyrogen-free per lot. Vented cap PTFE membranes are 0.22 &micro;m with an oversized vent area that increases gas exchange efficiency vs. standard-diameter membrane caps. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. Flask catalog sizes extend to high-efficiency 2 L and 5 L PC formats (NEST High Efficiency Erlenmeyer Flask line) for production-scale suspension culture. MBP stocks the NEST Scientific Erlenmeyer flask line across all major volumes and configurations. 
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Support accurate mixing and culturing with premium Erlenmeyer flasks designed for modern laboratories. <a href="https://www.mbpinc.net/contact-us" title="Contact the MBP Team" style="color: #236fa1; text-decoration: underline;">Contact the MBP team</a>&nbsp;for a formal quote.

Flyer

Erlenmeyer Flask with Bi-Directional Transfer Cap TPE Tube, Heat seal, Vent Filter

NEST Erlenmeyer Flask with Bi-directional Transfer Cap: 250 mL Erlenmeyer flask with bi-directional transfer cap, 50 cm Weldable TPE tube (1/8&quot; ID &times; 1/4&quot; OD), PTFE internal tube, 0.22 &mu;m PTFE vent filter (13.8 cm&sup2;), factory heat-sealed, E-beam sterile SAL 10&minus;6, ISO 13485 facility, DNase/RNase/pyrogen-free. 1/pk, 4/cs. Available in 250mL (C30921-AZC050A), 1000mL (C31121-AZC050A), or 2L high efficiency (C10122-AZB050A).

2L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

250mL Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (50cm 1/8" ID 1/4" OD) , Heat seal ,Vent Filter (0.22 μm 13.8cm²), Sterile, 1/pk, 4/cs

1000 mL Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (50cm1/8" ID 1/4" OD), Heat seal , Vent Filter (0.22 μm 13.8cm2), Sterile, 1/pk, 4/cs

<h2 dir="ltr">What are PC Erlenmeyer Flasks?</h2>
 PC (polycarbonate) Erlenmeyer flasks are autoclavable, shatter-proof, optically clear shake flasks manufactured from USP Class VI medical-grade polycarbonate for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. Polycarbonate provides a working temperature range of &minus;135 &deg;C to +135 &deg;C and withstands 5&ndash;10 autoclave cycles at 121 &deg;C &mdash; the defining advantage of PC over PETG for labs that steam-sterilize media or require reusable, re-sterilizable shake flasks. PC Erlenmeyer flasks are the correct choice when autoclavability, reuse, or processing at elevated temperature (&gt;60 &deg;C) is required; for single-use BPA-free workflows, PETG Erlenmeyer flasks are the alternative.
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<h2 dir="ltr">What you will find:</h2>
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Vent Filter Caps featuring 0.22&micro;m hydrophobic membranes to facilitate continuous, sterile gas exchange while preventing contamination and evaporation.
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Seal Caps designed for airtight liquid storage, transport, and non-aerated incubation steps.
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Flat Bottom Flasks optimized for uniform heating and stability on standard orbital shakers.
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Baffled Bottom Flasks engineered with integrated "flutes" to increase turbulence and oxygen mass transfer, ideal for high-density yeast and bacterial cultures.
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Volume Scalability with a comprehensive range from 125mL pilots to 5L high-efficiency production vessels, allowing for a seamless transition from bench-scale research to bulk bioprocessing.
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High-Efficiency Wide-Mouth Designs facilitate&nbsp;rapid filling and easy access for large-volume pipetting and automated dispensers.
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<h2 dir="ltr"> How to Choose PC Erlenmeyer Flasks</h2>
 Flat Base vs. Baffled Base for Your Cell Line Flat-base PC flasks produce laminar rotational flow during orbital shaking, providing low-shear mixing appropriate for shear-sensitive primary cells, some hybridoma lines, and media preparation. Baffled-base PC flasks generate turbulence during shaking that substantially increases dissolved oxygen (DO) transfer &mdash; critical for fast-growing CHO-S, HEK293-F, Expi293, bacterial (E. coli, B. subtilis), and yeast cultures where DO depletion limits cell density and productivity. NEST Scientific offers both base geometries across all standard volumes from 125 mL to 3,000 mL, with High Efficiency baffled formats extending to 5,000 mL for production-scale operations. Vented Cap vs. Plug Seal Cap Vented caps (0.22 &micro;m hydrophobic PTFE membrane, HDPE housing) allow continuous gas exchange during shaker culture while maintaining a sterile barrier. The NEST oversized vented cap membrane provides greater gas transfer area than standard-dimension vented caps, supporting high-density cultures. Plug seal caps are gas-impermeable HDPE caps used for static media storage in PC flasks, transport between incubators, and autoclave cycles (caps must be loosened 1&ndash;1.5 turns before autoclaving to prevent pressure buildup). Never use a sealed plug cap on a flask in an orbital shaker incubator &mdash; medium acidification and DO depletion occur within hours. Volume Selection and Fill Ratio NEST Scientific PC flasks are available in 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, 3,000 mL, and 5,000 mL (High Efficiency). Working fill for shaker culture: 20&ndash;25% of nominal volume. Working volumes: 25&ndash;30 mL in 125 mL; 50&ndash;60 mL in 250 mL; 100&ndash;125 mL in 500 mL; 200&ndash;250 mL in 1,000 mL; 400&ndash;500 mL in 2,000 mL. Scale-up from T-flask to Erlenmeyer: transition from T75 (15&ndash;25 mL static) to a 125 mL PC baffled flask when moving to suspension-adapted cell lines requiring orbital shaking. Autoclave Protocol for PC Flasks PC Erlenmeyer flasks can be autoclaved at 121 &deg;C for 15&ndash;20 minutes (standard gravity cycle). Loosen cap 1&ndash;1.5 turns before autoclaving; retighten after cooling. Repeated autoclaving causes progressive hydrolytic degradation of carbonate linkages &mdash; inspect for crazing, discoloration, or dimensional distortion after each cycle and retire flask at first sign of damage. Do not use autoclaved PC flasks for vacuum applications. BPA Consideration Polycarbonate contains bisphenol A (BPA) as an inherent part of its monomer chemistry. For applications involving estrogen receptor&ndash;positive cell lines (MCF-7, T47D, ZR-75), primary endocrine cells, or long-term culture runs exceeding 14 days, PETG Erlenmeyer flasks (BPA-free) are the safer choice. For standard bacterial, yeast, CHO, and HEK293 suspension culture, trace BPA from USP Class VI PC does not affect results in published culture protocols. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PC Erlenmeyer flasks are manufactured from USP Class VI medical-grade PC resin in ISO 8 cleanrooms, gamma sterilized per ISO 11137 to SAL 10⁻⁶, and individually wrapped in sterile packaging. PC tensile strength: 55&ndash;75 MPa; maintains impact resistance &gt;50 J/m at &minus;40 &deg;C. Vented cap 0.22 &micro;m hydrophobic PTFE membrane confirmed non-pyrogenic. Flask base diameter and neck geometry are designed for compatibility with standard orbital shaker clips from Infors HT, New Brunswick Innova, and Thermo Scientific MaxQ platforms &mdash; confirm flask O.D. against your specific shaker clamp before bulk ordering. NEST Scientific PC Erlenmeyer flasks up to 5 L (High Efficiency line) are stocked by MBP.
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Connect with the <a href="https://mbpinc.net/contact-us" title="MBP team" style="color: #236fa1; text-decoration: underline;">MBP team</a> today to secure the exact volumes and cap configurations required for your laboratory&rsquo;s next breakthrough.

PC Erlenmeyer Flasks

Flat Bottom PC Conical Erlenmeyer Flasks

NEST Scientific flat-bottom PC Erlenmeyer flasks for shaker culture, ISO 10993 medical-grade PC, E-beam SAL 10⁻⁶, autoclavable to 121°C. Eight variants: 125/250/500/1,000 mL × seal cap (781101/782101/783101/784101) or PTFE vent cap (781111/782111/783111/784111). 6–24/cs.

125 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Seal Caps, Sterile,1/pk, 6/cs

125 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, Flat Bottom, Vent Filter Caps, Sterile,1/pk, 6/cs

Flat Bottom PC High Efficient Erlenmeyer Flasks

NEST Flat-Bottom PC High Efficiency Erlenmeyer Flask: 2 L flat-bottom polycarbonate (ISO 10993) high-efficiency Erlenmeyer flask. E-beam sterile SAL 10&minus;6, DNase/RNase-free, autoclavable to 121&deg;C. Plug seal or vent cap. 1/pk, 4/cs. For mammalian and insect cell culture on orbital shakers. Available in 2L (785101 seal cap, 785111 vent cap), 3L (786101 seal cap, 786111 vent cap), and 5L (787001 seal cap, 787011 vent cap).

2L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

2L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

<h2 dir="ltr"> What are PETG Erlenmeyer Flasks?</h2>
 PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are single-use, sterile, BPA-free conical shake flasks manufactured from glycol-modified PET for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. PETG offers optical clarity comparable to PC, a working temperature range of &minus;80 &deg;C to +60 &deg;C, superior impact resistance at low temperatures versus standard PET, and a BPA-free composition &mdash; advantages over PC in single-use workflows where autoclaving is not required, and BPA-sensitive cell culture applications are involved.&nbsp; PETG Erlenmeyer flasks are the correct choice for high-throughput single-use suspension culture, BPA-free applications, and labs that want to eliminate autoclave and washing steps in their shake flask workflow.
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<h2 dir="ltr">What you will find:</h2>
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125mL PETG Erlenmeyer Flasks for Screening Studies: Perfect for small-scale media optimization and high-speed orbital shaking.
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250mL PETG Erlenmeyer Flasks for Routine Expansion: Provides a stable environment for consistent cell growth and passage maintenance.
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500mL PETG Erlenmeyer Flasks for Batch Production: Optimized for increasing cell densities while maintaining superior gas exchange.
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1000mL PETG Erlenmeyer Flasks for Large-Scale Recovery: Engineered for high-volume bioprocessing and bulk reagent preparation.
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<h2 dir="ltr"> How to Choose PETG Erlenmeyer Flasks</h2>
 PETG vs. PC for Shake Flask Culture PETG flasks are single-use and cannot be autoclaved (maximum temperature +60 &deg;C). PC flasks can be autoclaved 5&ndash;10 times and have a broader temperature range (&minus;135 &deg;C to +135 &deg;C). For high-throughput suspension culture operations processing many flasks per week, single-use PETG eliminates all washing, autoclave queuing, and glassware inspection steps &mdash; reducing hands-on time and contamination risk from reuse. For labs with lower throughput that prepare media in-house via autoclave, PC remains the cost-effective choice. PETG is BPA-free; PC contains BPA. Cell Line Compatibility PETG Erlenmeyer flasks are validated for CHO-S, HEK293-F, Expi293, Jurkat, K562, Sf9 (Spodoptera frugiperda), Hi5 (Trichoplusia ni), S. cerevisiae, P. pastoris, and E. coli suspension culture. The non-treated PETG surface prevents inadvertent cell adhesion and is compatible with all standard suspension culture media, including FreeStyle CHO, Expi293, Sf-900, and ESF 921 insect cell media. PETG is chemically resistant to DMSO, ethanol, isopropanol, and standard aqueous buffers. Baffled vs. Flat Base for PETG Flasks Baffled-base PETG Erlenmeyer flasks are the standard for oxygen-demanding suspension cultures: CHO, HEK293-F, bacterial, and insect cell lines. The baffles generate turbulent flow during orbital shaking, raising dissolved oxygen and reducing cell sedimentation. Flat-base PETG flasks are used for media preparation, gentle mixing, and shear-sensitive cell lines where turbulent conditions cause cell damage. NEST Scientific offers baffled and flat-base versions in both 125 mL and 1,000 mL. Vented Cap Gas Exchange NEST Scientific PETG Erlenmeyer flasks ship with a 0.22 &micro;m hydrophobic PTFE membrane vented cap in an HDPE housing with an oversized vent area. For suspension culture with 20&ndash;25% fill volume, vented caps at standard orbital speeds (100&ndash;150 RPM) provide adequate oxygen mass transfer to support mammalian cell densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting higher densities or with very high specific oxygen uptake rates (sOUR), confirm dissolved oxygen by inline monitoring before scaling further in PETG flasks. Storage of Unused Sterile Flasks Store unused NEST Scientific PETG Erlenmeyer flasks at room temperature (15&ndash;30 &deg;C) in the original sealed sterile bag, away from UV light. Shelf life is typically 3 years from the production date per the lot certificate. Inspect the bag seal before use; a compromised seal invalidates sterility. Remove from the bag inside a biosafety cabinet immediately before inoculation. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG Erlenmeyer flasks are manufactured from USP Class VI medical-grade PETG in ISO 8 cleanrooms. E-beam sterilized to SAL 10⁻⁶; each lot certified DNase/RNase-free, pyrogen-free, non-cytotoxic. Temperature range: &minus;80 &deg;C to +60 &deg;C. HDPE cap with 0.22 &micro;m PTFE hydrophobic membrane; oversized vent area for improved gas transfer. NEST Scientific is ISO 9001:2015 and ISO 13485 certified; FDA registered. NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL with baffled and flat-base options are stocked by MBP. 
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PETG Erlenmeyer Flasks

PETG Erlenmeyer Flask, Flat base

Flat Bottom PETG Erlenmeyer Flasks

Flat Bottom PETG Erlenmeyer Flask by NEST Scientific — 125 mL flat-bottom PETG Erlenmeyer flask with USP Class VI plug seal cap. E-beam sterile (SAL 10-6), cGMP/ISO 13485 facility, pyrogen-free, DNase/RNase-free. 1/pk, 24/cs. Available in 125 mL (781001/781011), 250 mL (782001/782011), 500 mL (783001/783011), and 1000 mL (784001/784011); seal or vent cap.

125ml PETG Erlenmeyer Flask, Flat base, Plug Seal Cap, Sterile,1/pk, 24/cs

250ml PETG Erlenmeyer Flask, Flat base, Plug Seal Cap, Sterile,1/pk, 12/cs

500ml PETG Erlenmeyer Flask, Flat base, Plug Seal Cap, Sterile,1/pk, 12/cs

1000ml PETG Erlenmeyer Flask, Flat base, Plug Seal Cap, Sterile,1/pk, 6/cs

125ml PETG Erlenmeyer Flask, Flat base, Vent Cap, Sterile,1/pk, 24/cs

250ml PETG Erlenmeyer Flask, Flat base, Vent Cap, Sterile,1/pk, 12/cs

500ml PETG Erlenmeyer Flask, Flat base, Vent Cap, Sterile,1/pk, 12/cs

1000ml PETG Erlenmeyer Flask, Flat base, Vent Cap, Sterile,1/pk, 6/cs

High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube & Vent Filter

NEST Scientific autoclavable polycarbonate baffled Erlenmeyer flasks with bi-directional TPE tube transfer cap and 0.22 µm vent filter. Three volumes: 2 L (C20122-AZB050A), 3 L (C20222-AZB050A), 5 L with large membrane vent (C10442-AKA060A, formerly 787081). E-beam sterile SAL 10⁻⁶. 4/cs.

2L High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

3L High Efficiency Erlenmeyer Flask with Baffles, with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

(old item # 787081) 5L High Efficiency Erlenmeyer Flask with Large Membrane Size Bi-directional Transfer Cap with TPE Tube (60cm 1/8" ID 1/4" OD), Sterile, 1/pk, 4/cs

High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube, Male CPC Connector & Vent Filter

NEST High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap — complete sterile flask-and-cap bundle (1/pk, 4/cs) in two volumes: C10223-DBE060A (3 L) and C10423-DBE060A (5 L). Both feature TPE tube (60 cm, 1/4&#34; ID), male CPC connector, and 0.22 μm vent filter (20 cm&#178;); E-beam SAL 10&#8315;&#8706;, ISO 13485.

3L High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (60cm 1/4" ID 7/16" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-Directional Transfer Cap, TPE Tube (60cm 1/4" ID 7/16" OD), Male CPC Connector with Female Sealing Cap ,Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 4/cs

High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube & Vent Filter

High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap by NEST Scientific — 3 L high efficiency Erlenmeyer flask with bi-directional transfer cap, 50 cm TPE tube (1/8&quot; ID &times; 1/4&quot; OD), 0.22 &mu;m &times; 24 mm vent filter. Sterile, 1/pk, 4/cs. Available as C10222-AZB050A (3L, 50cm tube), C10422-AZB050A (5L, 50cm tube), or C10422-AZB090A (5L, 90cm tube).

3L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap, TPE Tube (50cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (90cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

High Efficiency Erlenmeyer Flask by NEST Scientific — 2L high efficiency Erlenmeyer flask, USP Class VI polycarbonate, with bi-directional transfer cap, TPE tube (50cm, 1/4&quot; ID &times; 3/8&quot; OD), 0.22 &mu;m PTFE vent filter. E-beam sterilized, DNase/RNase-free. 1/pk, 4/cs. Available in 2 L (C10123-BZC050A), 3 L (C10223-BZC050A), and 5 L (C10423-BZC090A).

2L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm) , Sterile, 1/pk, 4/cs

3L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), , Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask with Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), Sterile, 1/pk, 4/cs

PC Conical Erlenmeyer Flasks, with Baffles

The NEST Scientific 781201 Series PC Erlenmeyer Shaker Flasks are polycarbonate (PC) Erlenmeyer flasks with baffled or flat-base bottom, USP Class VI-compliant, sterile, autoclavable to 121 °C, with a polypropylene seal cap. The baffled geometry enhances dissolved oxygen transfer and cell suspension uniformity in orbital shaker culture. Ships 24 per case. Also available in 250 mL, 500 mL, and 1,000 mL. Available in six volumes (125 mL, 250 mL, 500 mL, 1000 mL, 2 L, 3 L) each with Seal Cap or Vent Filter Cap — twelve SKUs total, all sterile, 1/pk.

125 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, with Baffles, Seal Caps, Sterile,1/pk, 6/cs

2L PC Conical Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 6/cs

3L PC Conical Erlenmeyer Flasks, Flat Base, Seal Cap, Sterile, 1/pk, 4/cs

125 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 24/cs

250 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 12/cs

500 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 12/cs

1000 mL PC Conical Erlenmeyer Flasks, with Baffles, Vent Filter Caps, Sterile,1/pk, 6/cs

2L PC Conical Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 6/cs

3L PC Conical Erlenmeyer Flasks, Flat Base, Vent Filter Cap, Sterile, 1/pk, 4/cs

PC High Efficient Erlenmeyer Flasks, with Baffles

PC Baffled High Efficiency Erlenmeyer Flask by NEST Scientific — 2 L USP Class VI polycarbonate high efficiency Erlenmeyer flask with baffled bottom and plug seal cap. E-beam sterile (SAL 10-6), cGMP/ISO 13485 facility, double-bagged. 1/pk, 4/cs. Available in 2L (785105 seal cap, 785115 vent cap), 3L (786105 seal cap, 786115 vent cap), and 5L (787005 seal cap, 787015 vent cap).

2L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

2L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

3L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, with Baffles, Seal Cap, Sterile, 1/pk, 4/cs

5L PC High Efficient Erlenmeyer Flasks, with Baffles, Vent Filter Cap, Sterile, 1/pk, 4/cs

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