Biofactory Systems

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
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BioFactory media transfer caps
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Sterile tubing and connector sets
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Fluid transfer adapters for cell culture vessels
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BioFactory bottle caps and closures
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Sterile quick-connect systems
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Liquid handling accessories
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Vessel handling and support tools
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Closed-system connectors for aseptic processing
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<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
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Biofactory System Accessories

Product Literature

T Shape CPC Connector for Inner Dia #3/8 Hose, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST Scientific 749001: sterile T-shape CPC connector for 3/8" ID hose in single-use bioprocess systems. Lateral 90° port for sampling, bypass, or branching while main flow continues. Individually wrapped, 1/pk, 5/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

Manufacturer

Vent Filter Specifications

Vent Filter，0.22 μm, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST 742001: 0.22 &mu;m vent filter, 62 mm diameter, sterile, individually wrapped. Provides sterile gas exchange on NEST BioFactory systems and Erlenmeyer flask cap assemblies. 1/pk, 5/cs.

Vent Filter，0.22 μm，62 mm diameter, Individually Wrapped, Sterile, 1/pk, 5/cs

Vent Filter，0.22 μm，56 mm diameter, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST BioFactory Caps Specifications

Wide Mouth BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

NEST Scientific 740001 is a 1-chamber Wide Mouth BioFactory with 647 cm² tissue-culture-treated growth surface. Sterile, individually wrapped, 1/pk, 10/cs. Plug or vent cap options available. For large-scale adherent cell culture, viral vector production, and cell expansion workflows.

Wide Mouth Plug Seal Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Wide Mouth Vent Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Narrow Mouth Plug Seal Caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Narrow Mouth Vent caps for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Y Shape CPC Connector for Inner Dia #3/8 Hose, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST Scientific 751001: sterile Y-shape CPC connector for 3/8" ID hose in single-use bioprocess fluid systems. Splits or combines two fluid lines in a closed system. Individually wrapped, 1/pk, 5/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

<h2 dir="ltr"> What are NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; Systems are compact, single-use, multi-layer cell culture vessels manufactured from USP Class VI polystyrene for large-scale adherent cell culture. The BioFactory provides the growth kinetics and cell attachment characteristics of standard T-flasks, scaled up to growth areas from approximately 636 cm&sup2; (1-layer) to 25,440 cm&sup2; (40-layer) in a single vessel &mdash; making it the scale-up path from T-flask and roller bottle culture for vaccine production, monoclonal antibody manufacturing, viral vector production (lentivirus, AAV, adenovirus), and biopharmaceutical protein expression from adherent cell lines.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<li>BioFactory systems: Multi-layer sterile cell culture vessels available with different chamber configurations, plug seal cap options, transfer caps, vent filters, and frost-resistant models for large-scale adherent cell culture.</li>
<li><a href="https://mbpinc.net/consumables/cell-culture/biofactory-systems/biofactory-system-accessories">BioFactory System Accessories:</a> Adaptor caps, overcaps, holders, vent filters, accessory packages, tubing sets, and preconfigured kits designed to support media handling and system operation.</li>
<li>Sterile fluid transfer components: CPC connectors, T- and Y-shaped connectors, sealing plugs, adaptor connectors, hose clamps, funnels, and filter/tubing assemblies for closed liquid transfer workflows.</li>
<li>Bioprocess tubing: Sterile C-Flex&reg; welding hose, SPT-50 hose, and inlet/outlet tubing compatible with BioFactory systems and media transfer applications.</li>
<li>Preassembled BioFactory configurations: Ready-to-use systems equipped with integrated vent filters, transfer tubing, or specialized cap assemblies to streamline cell culture setup.</li>
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<h2 dir="ltr"> How to Choose the Right BioFactory&trade; Layer Count</h2>
 Matching BioFactory Scale to Production Stage The 1-layer BioFactory (&asymp;636 cm&sup2;) is equivalent in surface area to approximately 8&ndash;9 standard T75 flasks &mdash; used for proof-of-concept scale-up studies transitioning from T-flask monolayer culture. The 2-layer BioFactory (&asymp;1,272 cm&sup2;) is the standard for pilot-scale viral antigen production and early-phase process development. The 5-layer (&asymp;3,180 cm&sup2;) and 10-layer (&asymp;6,360 cm&sup2;) formats suit mid-scale production runs for research-grade vaccine lots, transfection-derived protein, and pre-clinical viral vector batches. The 40-layer (&asymp;25,440 cm&sup2;) format is an industrial-scale format for commercial vaccine, mAb, and biopharmaceutical production. Closed Liquid Transfer and Contamination Reduction NEST BioFactory&trade; systems feature a narrow-mouth cap port compatible with aseptic tubing for closed liquid transfer &mdash; media, wash buffers, trypsin, and harvest supernatant can be introduced and removed through the tube port without opening the vessel. This closed-system operation eliminates the open-pour steps that create contamination risk in multi-layer roller bottle workflows and enables BSL-2 containment of pathogen-bearing cultures without biosafety cabinet&ndash;dependent open-handling. Wide-mouth caps allow direct media pouring for non-closed workflows. TC Treatment and Cell Compatibility All NEST BioFactory&trade; layers are TC-treated by vacuum plasma using the same surface treatment process as NEST cell culture T-flasks. NEST has published VERO cell culture data showing that cell morphology, adherence, and growth kinetics in the BioFactory are equivalent to those in standard T-flasks &mdash; cell attachment density, proliferation rate, and morphology are not altered by the scale-up. This linear scalability is the primary engineering advantage of multi-layer vessels over microcarrier bioreactors and packed-bed systems for adherent cell&ndash;derived products requiring 2D culture validation. Ultrasonic Welding and Particle Generation The BioFactory layers are joined by ultrasonic welding without chemical adhesives or extrinsic ingredients. NEST's welding line design minimizes particle generation at the weld joints, an important quality attribute for pharmaceutical production contexts where subvisible particle counts in harvest media must meet compendial limits. Automation and GMP Compatibility NEST BioFactory&trade; systems are compatible with automated media handling equipment. Multiple BioFactory units can be connected in series through the tubing ports for further scale-out. The double-bagged sterile packaging format supports GMP cleanroom entry requirements. NEST provides validation documentation packages (sterilization data, extractable/leachable certificates, biocompatibility reports) supporting GMP authorization for industrial cell culture production. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; Systems are manufactured from USP Class VI polystyrene in a Class 10,000 (ISO 7) dedicated cleanroom. All culture surfaces are TC-treated by vacuum plasma. Temperature range: approximately &minus;86 &deg;C to +64 &deg;C. E-beam sterilized to SAL 10⁻⁶; non-pyrogenic; double-bagged sterile packaging. Vent caps contain 0.22 &micro;m hydrophobic filters for gas exchange without contamination. VERO cell culture validation data available from NEST Scientific: seeding at 5 &times; 10⁴/mL in 2-layer BioFactory (400 mL) shows robust, even adherence and growth kinetics matching T-flask controls after 3 days. Cell lines validated: Vero, MRC-5, 2BS, 293T, L-929, and equivalent adherent production lines. NEST Scientific BioFactory&trade; systems across all layer configurations are carried by MBP. 
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DataSheet

NEST BioFactory Systems with 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap Specifications

NEST BioFactory Systems with 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap

773001 by NEST Scientific — 1-chamber BioFactory system with 647 cm² TC-treated growth surface. Ships with wide and narrow plug seal caps for closed bioprocess liquid transfer. Sterile, 1/pk.

Nest BioFactory 1 Chamber, 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap, Total culture area: 647cm2, TC, Sterile, 1/pk, 8/cs (with 8 sterile wide vent caps and 8 sterile narrow vent caps included separately)

Nest BioFactory 2 Chamber, 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap, Total culture area: 1279cm2, TC, Sterile, 1/pk, 8/cs (with 8 sterile wide vent caps and 8 sterile narrow vent caps included separately)

Nest BioFactory 5 Chamber, 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap, Total culture area: 3175cm2, TC, Sterile, 1/pk, 4/cs (with 4 sterile wide vent caps and 4 sterile narrow vent caps included separately)

Nest BioFactory 10 Chamber, 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap, Total culture area: 6335cm2, TC, Sterile, 1/pk, 6/cs (with 6 sterile wide vent caps and 6 sterile narrow vent caps included separately)

Nest BioFactory 40 Chamber, 1 Wide Plug Seal Cap + 1 Narrow Plug Seal Cap, Total culture area: 25295cm2, TC, Sterile, 1/pk, 2/cs (with 2 sterile wide vent caps and 2 sterile narrow vent caps included separately)

NEST BioFactory Systems with 2 Narrow Plug Seal Caps Specifications

NEST BioFactory Systems with 2 Narrow Plug Seal Caps

772001 by NEST Scientific is a single-use, sterile BioFactory™ 1-layer cell culture system with 647 cm² TC-treated polystyrene growth surface and 2 narrow plug seal caps. Designed for mammalian cell scale-up, vaccine production, mAb manufacturing, and biopharmaceutical process development; available 1/pack, 8/case.

Nest BioFactory 1 Chamber; 2 Narrow Plug Seal Caps; Total culture area: 647cm2; TC; sterile, 1/pk, 8/cs (with 16 sterile narrow vent caps included separately)

Nest BioFactory 2 Chamber; 2 Narrow Plug Seal Caps; Total culture area: 1279cm2; TC; sterile, 1/pk, 8/cs (with 16 sterile narrow vent caps included separately)

Nest BioFactory 5 Chamber; 2 Narrow Plug Seal Caps; Total culture area: 3175cm2; TC; sterile, 1/pk, 4/cs (with 8 sterile narrow vent caps included separately)

Nest BioFactory 10 Chamber; 2 Narrow Plug Seal Caps; Total culture area: 6335cm2; TC; sterile, 1/pk, 6/cs (with 12 sterile narrow vent caps included separately)

Nest BioFactory 40 Chamber; 2 Narrow Plug Seal Caps; Total culture area: 25295cm2; TC; sterile, 1/pk, 2/cs (with 4 sterile narrow vent caps included separately)

NEST BioFactory Systems with 2 Wide Plug Seal Caps Specifications

NEST BioFactory Systems with 2 Wide Plug Seal Caps

771001 by NEST Scientific is a single-chamber BioFactory™ cell culture system with 632 cm² of TC-treated growth area and 2 wide plug seal caps. E-beam sterilized to SAL 10⁻⁶, certified pyrogen-free and BSE/TSE-free, manufactured in an ISO 7 cleanroom to ISO 13485:2016 standards. Supplied 1/pk, 8/cs. Scalable to 2-, 5-, 10-, and 40-chamber configurations for bioproduction and vaccine work.

Nest BioFactory 1 Chamber; 2 Wide Plug Seal Caps; Total culture area: 647cm2; TC; sterile, 1/pk, 8/cs (with 16 sterile wide vent caps included separately)

Nest BioFactory 2 Chamber; 2 Wide Plug Seal Caps; Total culture area: 1279cm2; TC; sterile, 1/pk, 8/cs (with 16 sterile wide vent caps included separately)

Nest BioFactory 5 Chamber; 2 Wide Plug Seal Caps; Total culture area: 3175cm2; TC; sterile, 1/pk, 4/cs (with 8 sterile wide vent caps included separately)

Nest BioFactory 10 Chamber; 2 Wide Plug Seal Caps; Total culture area: 6335cm2; TC; sterile, 1/pk, 6/cs (with 12 sterile wide vent caps included separately)

Nest BioFactory 15 Chamber, -20C Frost Resistance, 2 Wide Plug Seal Caps, TC, Sterile, 1/pk, 6/cs (with 12 Sterile Vent Caps Included Separately)

Nest BioFactory 40 Chamber, 2 Wide Plug Seal Caps; TC, Sterile, 25295cm2, Sterile,1/pk, 2/cs (with 4 sterile wide vent caps included separately)

NEST BioFactory Systems with 2 Solid Overcaps Specifications

NEST BioFactory Systems with 2 Solid Overcaps

771422 by NEST Scientific — 40-chamber BioFactory cell culture system, 25,295 cm&sup2; total culture area, USP Class VI virgin polystyrene, TC-treated, 2 solid overcaps + 4 vented overcaps, E-beam sterile. 1/pk, 2/cs.

Nest Biofactor 40 Chamber with 2 Solid Overcaps, Total Culture Area: 25295 cm2, TC, Sterile, 1/pk, 2/cs (with 4 Sterile Vented Overcaps Included Separately)

Nest Biofactor 10 Chamber with 2 Solid Overcaps, Total Culture Area: 6335 cm2, TC, Sterile, 1/pk, 6/cs (with 12 Sterile Vented Overcaps Included Separately)

NEST BioFactory Systems with Filter and Transfer Cap,TPE Tube

BZD080A by NEST Scientific is a 5-chamber BioFactory cell culture system (3,160 cm² total area) pre-installed with a 0.22 µm PTFE vent filter and 80 cm TPE transfer tube (1/4" ID, 3/8" OD). E-beam sterile SAL 10⁻⁶, pyrogen-free, animal-product-free, 1/pk, 2/cs. Eliminates manual assembly for large-scale adherent cell culture. Order from MBP, a registered vendor to Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

1 Chamber BioFactory with Filter and Transfer Cap,TPE Tube (80cm 1/4" ID 3/8" OD) , Heat seal ,Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 2/cs

2 Chamber BioFactory with Filter and Transfer Cap,TPE Tube (80cm 1/4" ID 3/8" OD) , Heat seal ,Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 2/cs

5 Chamber BioFactory with Filter and Transfer Cap,TPE Tube (80cm 1/4" ID 3/8" OD) , Heat seal ,Vent Filter (0.22 μm 20cm²), Sterile, 1/pk, 2/cs

10 Chamber BioFactory Pre Installed with a Vent Filter (0.22 μm 62 mm Diameter) and a 80 mm Hose(1/4" ID, 3/8" OD )

C71554-BZD080A by NEST Scientific is a 10-chamber BioFactory™ cell culture system pre-installed with a 0.22 µm PTFE vent filter (50 mm) and a 60 cm TPE transfer hose (1/4″ ID, 3/8″ OD), providing 6,320 cm² of TC-treated growth surface ready for sterile media and cell transfer without additional assembly. E-beam sterilized SAL 10⁻⁶, ISO 13485:2016, 2 units per case.

BioFactory (-20°C Frost Resistance) Specifications

NEST BioFactory Systems with -20C Frost Resistance, 2 Wide Plug Seal Caps

771392 by NEST Scientific is a 10-chamber BioFactory cell culture system with 6,320 cm² TC-treated surface, sterile, with -20 °C frost resistance validated for three freeze-thaw cycles. Includes 2 wide plug seal caps; 12 sterile vent caps shipped separately. Dimensions: 335 × 205 × 201 mm. Cases of 6 units.

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NEST BioFactory Adaptor Caps Specifications

Adaptor Caps for BioFactories, Individually Wrapped, Sterile

NEST Scientific 740201 sterile adaptor caps for BioFactory multi-layer cell culture vessels, individually wrapped. Enables closed-system tubing and hose connections to NEST BioFactory ports for media exchange and harvest in large-scale adherent cell culture. Multiple cap types and materials available.

Adaptor Cap Wide Mouth to Narrow Mouth for BioFactories, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 1/4" (5.8 mm) ID Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

Adaptor Cap Wide Mouth to 3/8" ID (9.5 mm) Hose for BioFactories, HDPE, Individually Wrapped, Sterile, 1/pk, 10/cs

United States

Texas

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