MagBio Reagents

Mag Beads

<h2>What are Magnetic Bead Viral RNA Extraction Kits?</h2>
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Magnetic bead viral RNA extraction kits purify viral RNA from cell-free biological matrices - plasma, serum, nasopharyngeal swabs, urine, saliva, blood, and cell culture supernatant - by binding viral nucleic acids to paramagnetic silica-coated beads during chaotropic lysis. A magnetic field captures bead-RNA complexes during wash steps, eliminating centrifugation, and purified viral RNA elutes in 20-50 ul of RNase-free water or elution buffer suitable for direct use in RT-qPCR, RT-LAMP, and next-generation sequencing. The 96-well plate format processes all samples in a single run on a compatible liquid-handling robot, making this the standard choice for surveillance programs, diagnostic batch processing, and clinical biobank workflows. Choose a magnetic bead viral RNA kit when daily sample volumes exceed 24 extractions per session, when automation infrastructure is available, or when viral loads are low and maximum sensitivity from a small elution volume is required.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Magbio HighPrep Viral RNA Kit KingFisher for seamless, automated viral RNA extraction using specialized superparamagnetic bead technology.</li>
<li>KingFisher-compatible magnetic bead workflows designed for high-throughput pathogen processing.</li>
<li>Automation-ready viral RNA purification solutions that reduce manual handling and improve laboratory efficiency.</li>
<li>Magnetic bead-based extraction systems for reliable recovery of viral nucleic acids from diverse sample types.</li>
<li>High-quality viral RNA isolation products suitable for RT-PCR, qPCR, sequencing, and molecular diagnostics applications.</li>
<li>Scalable pathogen extraction kits optimized for routine testing, surveillance, and research workflows.</li>
</ul>
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<h2>How to Choose a Magnetic Bead Viral RNA Extraction Kit</h2>
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Automation Platform Compatibility
Confirm the kit provides validated automation scripts for your instrument before purchase. Zymo Research Quick-DNA/RNA Viral MagBead kits support Tecan Fluent, Hamilton STAR, and Beckman Biomek; Thermo Fisher MagMAX Viral/Pathogen kits are validated on the KingFisher Flex and Duo systems. Generic bead kits without platform-specific scripts require in-house protocol development that adds weeks of validation time.
RNA-Only vs. DNA/RNA Co-Extraction
For pan-pathogen surveillance panels covering both RNA viruses (influenza, SARS-CoV-2, RSV) and DNA viruses (EBV, CMV, adenovirus, HPV), select a kit explicitly validated for DNA/RNA co-extraction. RNA-only kits use binding conditions that discriminate against dsDNA and will underperform on DNA viral targets.
Sample Input Volume and Matrix
Standard magnetic bead viral RNA kits support 100-200 ul of plasma, serum, or swab eluate per well. For low-viral-load samples where concentrating a larger starting volume is critical, verify the kit protocol for 500 ul input per well and confirm bead volume scales proportionally. Samples stabilized in Zymo DNA/RNA Shield can be directly loaded without an additional inactivation step.
Elution Volume
A 20-50 ul elution concentrates viral RNA from a standard 200 ul input by 4-10-fold relative to the original matrix, improving RT-qPCR sensitivity by 0.5-1.5 Ct. For sequencing applications, a 50-100 ul elution recovers more total RNA mass, which is more important than concentration for library input requirements.
Carrier RNA
Confirm the kit includes carrier RNA in the lysis buffer for low-titer specimens. For samples with expected viral copies &lt; 1x10^3/ml, carrier RNA supplementation is not optional - it is the difference between detectable and non-detectable results.
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<h2>Specifications Context</h2>
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Performance benchmarks: &gt;=70% RNA recovery measured by spike-in controls, eluate A260/A280 &gt;= 1.7, and Ct within 2 cycles of reference extraction. Magnetic bead-based extraction has been benchmarked against the QIAGEN QIAcube commercial platform and shown comparable RT-qPCR sensitivity for SARS-CoV-2 N-gene and E-gene targets, as reported in published peer-reviewed diagnostic studies. Zymo Research Quick-DNA/RNA Viral MagBead kits (R2140-E, R2141-E) hold Emergency Use Authorization and CE-IVD designation for diagnostic use, and are used in SARS-CoV-2 clinical workflows. Store extracted viral RNA at -70C to -80C; pre-extraction sample stabilization with DNA/RNA Shield enables ambient-temperature transport for up to 30 days without loss of RNA integrity.
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
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HighPrep Viral RNA Kit (KF Format)

Product Fact Sheet - HighPrep Viral RNA Kit

Magbio HighPrep Viral RNA Kit KingFisher

The HighPrep Viral RNA Kit KingFisher Format by MagBio Genomics extracts viral RNA from 200 µL of nasopharyngeal swabs, whole blood, serum, plasma, and saliva in 23 minutes on KingFisher™ Flex (MVP_2Wash_200_Flex program). No organic solvents required. Optimized for SARS-CoV-2 RNA isolation. Compatible with RT-qPCR, PCR, NGS, and viral load monitoring workflows. Available in 96-prep (KFV-R96) and 2000-prep (KFV-R2000) sizes.

Features

Main functions

Starting material (Sample matrix)

Sample amount (Input)

Downstream application

Process method

Instrument

Purification method

Purification time

Manufacturer

HighPrep Viral RNA Kit KingFisher™ Format (96 Preps)

HighPrep Viral RNA Kit KingFisher™ Format (2000 Preps)

<h2>What are Magnetic Bead Viral DNA/RNA Co-Extraction Kits?</h2>
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Magnetic bead viral DNA/RNA co-extraction kits purify both viral DNA and RNA from plasma, serum, urine, nasopharyngeal swabs, saliva, blood, cellular suspensions, biopsies, fecal samples, and cell culture media stored in DNA/RNA Shield using paramagnetic silica-coated beads that bind nucleic acids from &gt;50 nt to &gt;200 kb during a single chaotropic lysis step, with magnetic separation during successive wash steps removing inhibitors - no centrifugation required during purification. The 96-well format integrates with KingFisher Flex, Tecan Fluent, and Hamilton STAR liquid-handling platforms, enabling fully automated extraction of both RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, herpesvirus, parvovirus) from 96 samples per run. Choose the magnetic bead format for viral DNA/RNA co-extraction when processing 48+ samples per run, when a compatible automation platform is available, or when the program requires EUA-associated workflows validated for SARS-CoV-2 or other respiratory panel targets.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Magbio HighPrep Viral-Bacterial DNA &amp; RNA Kit (HVB-DR0): Scalable magnetic bead chemistry optimized for the simultaneous extraction of viral and bacterial nucleic acids from diverse fluid profiles.
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<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral DNA &amp; RNA Kit (HPV-DR0): High-efficiency magnetic isolation systems engineered for the rapid purification of viral target vectors from fluid specimens.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral-Pathogen DNA &amp; RNA Kit (HPP-DR0): Specialized bead technology designed to maximize pathogen recovery and eliminate inhibitors from challenging biological matrices.
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<li dir="ltr" style="color: #000000;" aria-level="1">
Quick-DNA/RNA Viral MagBead (R2140): Advanced magnetic-bead separation configurations built for fast, high-purity co-purification of total viral nucleic acids.
</li>
</ul>
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<h2>How to Choose a Magnetic Bead Viral DNA/RNA Co-Extraction Kit</h2>
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Automation Platform Compatibility
Confirm the kit's automation scripts are available for your specific platform before purchase. The Quick-DNA/RNA Viral MagBead Kit is used in Zymo Research's SARS-CoV-2 EUA workflow and is compatible with KingFisher and other major liquid-handling platforms; contact MBP's technical team or Zymo Research's automation support for current script availability. For Tecan Fluent or Hamilton STAR, platform-specific script development or adaptation may be required.
DNA/RNA Shield as the Collection Medium
The Quick-DNA/RNA Viral MagBead Kit is designed around DNA/RNA Shield as the sample collection, inactivation, and storage medium. Swabs or other samples stabilized in DNA/RNA Shield load directly into the extraction protocol without additional neutralization steps. For programs using VTM (viral transport medium), UTM, or PBS as collection media, confirm compatibility with the kit's lysis buffer by testing spike-in recovery before adopting at scale.
Carrier RNA Requirement
Unlike the QIAGEN QIAamp Viral RNA Mini Kit, the Quick-DNA/RNA Viral MagBead Kit does not require the user to prepare and add carrier RNA to the lysis buffer - the kit's buffer system is formulated for complete lysis and efficient nucleic acid binding without this step. Eliminating the carrier RNA preparation step reduces per-batch setup time and removes one source of inter-batch variability in high-throughput automated workflows.
Nucleic Acid Size Recovery Range
Confirm the kit recovers the size range needed for your downstream application. The Quick-DNA/RNA Viral MagBead Kit binds nucleic acids from &gt;50 nt to &gt;200 kb - covering small viral RNAs (miRNA-sized fragments from viral genomes), standard RT-qPCR amplicon targets, and full-length viral genomes for metagenomic NGS. Applications requiring recovery of very short fragments (&lt;50 nt) need a kit specifically validated for that range.
Regulatory Requirements
For IVD diagnostic use or regulatory submissions, confirm the kit's current regulatory status. The Quick-DNA/RNA Viral MagBead Kit has been used in EUA workflows; its current authorizations and intended uses may have evolved since initial authorization. Always verify directly with Zymo Research or MBP's regulatory affairs team for current compliance documentation.
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<h2>Specifications Context</h2>
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The Quick-DNA/RNA Viral MagBead Kit binds small (&gt;50 nt) and large (&gt;200 kb) DNA and RNA to magnetic beads and elutes high-quality nucleic acids ready for NGS, hybridization-based detection, and RT-qPCR. The kit is used in Zymo Research's SARS-CoV-2 EUA extraction workflow and has been adopted in published multiplex respiratory panel methods and pan-pathogen metagenomics protocols. A published PMC study on integrated DNA and RNA extraction from viral pathogens causing acute respiratory infections demonstrated that magnetic bead-based co-extraction achieved equivalent or superior sensitivity to spin-column formats for both RSV (RNA virus) and adenovirus (DNA virus) from the same nasopharyngeal swab input. Magnetic bead viral co-extraction is the standard format for large public health reference laboratories and clinical metagenomics programs processing 96-1,500+ samples per day across outbreak response and surveillance networks.
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Contact the helpful <a href="https://mbpinc.net/contact-us">team at MBP</a> today to request a quote for your specific research requirements.

HighPrep Viral/Bacterial DNA/RNA Kit

MSDS

Magbio HighPrep Viral-Bacterial DNA & RNA Kit

HighPrep Viral-Bacterial DNA &amp; RNA Kit by MagBio Genomics is a magnetic bead-based co-isolation system for viral and bacterial DNA and RNA from whole blood, serum, plasma, saliva, and nasopharyngeal swabs, running in 23 minutes on the KingFisher Flex platform — available in 5-prep (HVB-DR5), 96-prep (HVB-DR96), and 384-prep (HVB-DR96x4) formats.

HighPrep Viral-Bacterial DNA & RNA Kit (5 preps)

HighPrep Viral-Bacterial DNA & RNA Kit (96 preps)

HighPrep Viral-Bacterial DNA & RNA Kit (384 preps)

HighPrep Viral/Pathogen DNA/RNA Kit

Magbio HighPrep Viral-Pathogen DNA & RNA Kit

MagBio HighPrep Viral-Pathogen DNA & RNA Kit by MagBio Genomics: magnetic bead-based simultaneous extraction of viral, bacterial, and fungal DNA and RNA from blood, plasma, serum, saliva, urine, and swabs. MAG-S1 beads; ~52 min on KingFisher Flex with ready-made script. 96-prep and 384-prep formats. RT-qPCR, PCR, and NGS compatible. Available in 5-prep (HPP-DR5), 96-prep (HPP-DR96), and 384-prep (HPP-DR96x4) sizes.

HighPrep Viral-Pathogen DNA & RNA Kit (5 preps)

HighPrep Viral-Pathogen DNA & RNA Kit (96 preps)

HighPrep Viral-Pathogen DNA & RNA Kit (384 preps)

DNA normalization and Concentration

<h2>What is DNA normalization and concentration for NGS?</h2>
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DNA normalization for NGS is the process of adjusting all individual indexed sequencing libraries to the same molar concentration before pooling them into a single multiplexed sequencing run, so that each library contributes approximately the same number of sequencing reads to the total run output. Concentration may also be needed when a library is too dilute after cleanup or elution. SPRI (solid-phase reversible immobilization) magnetic bead-based cleanup, most commonly using AMPure XP beads, is the standard method for post-library-prep cleanup, size selection, and concentration in NGS workflows.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio MagQuant Plus DNA Kit: for automated, high-throughput nucleic acid concentration and normalization workflows
</li>
</ul>
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<h2>How to normalize and concentrate NGS libraries</h2>
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Quantify each library by qPCR to calculate molar concentration accurately
Kapa Library Quantification qPCR kits or equivalent measure the amplifiable, adapter-ligated molecules in a library, providing a molar concentration based on insert size that can be used to pool libraries at equal molar ratios. Fluorometric quantitation gives mass per volume but not molar concentration, and libraries with different insert sizes have different mass-to-moles conversion factors.
Calculate library molar concentration using measured mass and average insert size
If molar quantitation by qPCR is not available, the molar concentration of a library can be estimated from its fluorometric mass concentration and the average insert size from Bioanalyzer or TapeStation: molar concentration (nM) = (mass in ng/&micro;l &times; 10^6) / (insert size in bp &times; 660 g/mol/bp). This converts mass to moles using the average molecular weight per base pair.
Pool indexed libraries at equal molar ratios for balanced depth
After calculating the molar concentration of each library, pool volumes delivering equal moles of each library into the final pool. Even small quantitation errors translate into coverage imbalances between samples in the sequencing output.
Use SPRI bead cleanup to concentrate libraries and remove adapter dimers
SPRI bead cleanup with AMPure XP beads at the appropriate bead-to-sample ratio selects for DNA fragments above a size cutoff while removing smaller adapter dimers and free adapter molecules. The final elution in a smaller volume of buffer concentrates the library. The bead ratio determines the minimum retained fragment size, with higher ratios retaining smaller fragments.
Verify the pooled library before sequencing
After pooling and final SPRI cleanup, measure the pooled library by qPCR and Bioanalyzer to confirm molar concentration and size distribution before loading, since loading concentration determines cluster density and affects sequencing performance.
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<h2>Specifications context</h2>
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AMPure XP SPRI beads use polyethylene glycol and sodium chloride to precipitate DNA onto paramagnetic beads in a size-dependent manner, making the bead-to-sample volume ratio the key parameter for controlling the minimum retained fragment size. As of 2026, automated bead-based normalization kits that combine quantitation and normalization remain available for high-throughput settings, though most academic labs perform manual quantitation and normalization before pooling.
Browse through the catalog and <a href="https://mbpinc.net/contact-us">contact the expert team</a> to book high-quality DNA normalization and concentration products today.

MagQuant_Plus_DNA Kit V2 - Protocol_REV3

Magbio MagQuant Plus DNA Kit V2

MagQuant Plus DNA Kit V2 by MagBio Genomics is a magnetic bead kit for DNA normalization and quantitation in NGS, amplicon, and genomic DNA workflows — no standard curve, no centrifugation, 25&ndash;50&nbsp;&micro;L elution, 96/384-well compatible. Available in 10 (MQP-50010), 96 (MQP-50096), 384 (MQP-50384), 1,920 (MQP-51920), and 5,000 preps (MQP-55000) from MBP INC.