MagBio Reagents

MagBeads

<h2>What Are Magnetic Bead Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
Magnetic bead genomic DNA kits for blood and tissue use paramagnetic beads to capture genomic DNA released during sample lysis. Following DNA binding, a magnetic separator immobilizes the beads, and contaminants are removed by wash steps before purified DNA is eluted. Unlike spin-column workflows, magnetic bead protocols eliminate centrifugation and simplify automation, making them well-suited to high-throughput laboratories and automated liquid-handling systems.
Magnetic bead extraction is often selected when automation, reduced hands-on processing, or compatibility with high-throughput workflows is a priority. Specialized high-molecular-weight (HMW) magnetic bead chemistries are also available for applications requiring longer DNA fragments for advanced sequencing workflows.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA&trade; Magbead Plus Kit: Magnetic bead-based genomic DNA purification for blood, tissue, cultured cells, and other biological samples.</li>
<li>Automation-Compatible Workflows: Designed for seamless integration with manual and automated magnetic separation platforms.</li>
<li>High-Yield DNA Recovery: Optimized to produce purified genomic DNA suitable for PCR, qPCR, genotyping, sequencing, and other downstream molecular biology applications.</li>
<li>Streamlined Sample Processing: Eliminates centrifugation steps through magnetic bead technology, simplifying genomic DNA extraction workflows.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a Magnetic Bead Genomic DNA Kit for Blood and Tissue</h2>
&nbsp;
Sample Input and Processing Time
Most magnetic bead kits are designed to process approximately 100-400 &micro;L of whole blood or buffy coat per preparation. Tissue and cultured cell inputs vary by kit but generally support small tissue samples and several million cells per extraction. Manual workflows can often be completed in about 20 minutes because centrifugation steps are eliminated.
Yield and Purity Requirements
Magnetic bead extraction kits are capable of producing high-quality genomic DNA suitable for PCR, qPCR, genotyping, sequencing, Southern blotting, and related molecular biology applications. DNA purity is commonly comparable to silica-based purification methods, making these kits suitable for most downstream workflows.
Automation Compatibility
One of the primary advantages of magnetic bead chemistry is its compatibility with automated liquid handling platforms. Because DNA capture and washing occur through magnetic separation rather than centrifugation, these workflows can be readily adapted to 96-well and higher-throughput automated formats.
High-Molecular-Weight DNA Applications
Some magnetic bead systems are specifically optimized for high-molecular-weight genomic DNA recovery. These workflows can preserve larger DNA fragments required for long-read sequencing, linked-read assembly, structural variation analysis, and other applications where DNA integrity is critical.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Magnetic bead genomic DNA extraction kits are differentiated by their centrifuge-free workflow, automation compatibility, and flexibility across blood, buffy coat, tissue, and cultured cell samples. The magnetic separation process reduces manual handling steps while supporting consistent DNA recovery and scalable throughput.&nbsp;Many laboratories adopt magnetic bead workflows when transitioning from manual extraction methods to automated sample preparation systems. Compared with traditional spin-column methods, magnetic bead purification simplifies integration with robotic liquid handlers and high-throughput extraction platforms while maintaining DNA quality suitable for PCR, qPCR, sequencing, and genotyping applications.&nbsp;When selecting a kit, evaluate sample input requirements, automation compatibility, DNA purity specifications, and whether high-molecular-weight DNA recovery is required for downstream applications. Most kit reagents are stored according to manufacturer recommendations, and lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to get high-quality mag beads for blood/tissue genomic DNA extraction workflows to scale up your laboratory throughput.

Mag Beads

HighPrep™ Blood & Tissue DNA Kit - Protocol

MSDS

HighPrep™ Blood-Tissue DNA Kit Comparative Data

MagBio HighPrep Blood & Tissue DNA Kit

HighPrep Blood &amp; Tissue DNA Kit by MagBio Genomics uses MAG-S1 paramagnetic particles to purify genomic, mitochondrial, and viral DNA from blood (20&ndash;250&nbsp;&micro;L), tissue (up to 10&nbsp;mg), mouse tail (2&ndash;5&nbsp;mm), cultured cells, saliva, and buccal swabs via a 7-step Lyse&ndash;Bind&ndash;Wash&ndash;Elute protocol; A260/A280 &gt;1.8; 50&ndash;200&nbsp;&micro;L elution. Available in 5 preps (HPBTS-D5), 96 preps (HPBTS-D96), and 384 preps (HPBTS-D96x4) from MBP INC.

Features

Size Range

Purity

Yield

Manufacturer

HighPrep Blood & Tissue DNA Kit (5 preps)

HighPrep Blood & Tissue DNA Kit (96 preps)

HighPrep Blood & Tissue DNA Kit (384 preps)

Sanger DTR

<h2 data-section-id="vnaphp" data-start="0" data-end="28">What are Sanger DTR kits?</h2>
&nbsp;
Sanger DTR (Dye Terminator Removal) kits are post-sequencing cleanup products used to remove excess fluorescent dye terminators, unincorporated nucleotides, salts, and short primer fragments from completed cycle sequencing reactions before capillary electrophoresis. During Sanger sequencing, a large excess of fluorescently labeled dideoxynucleotide terminators (ddNTPs) is required to generate DNA fragments of varying lengths. After the reaction, most of these labeled terminators remain unused.
If these contaminants are not removed, they produce strong fluorescent signals known as dye blobs at the beginning of the electropherogram. Dye blobs can obscure the first portion of the sequence, reduce base-calling accuracy, and increase sequencing failure rates. DTR cleanup kits solve this problem by separating the desired labeled DNA fragments from smaller unwanted molecules prior to loading the sample onto a sequencing instrument.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
HighPrep DTR Magnetic Reagents: A robust paramagnetic bead-based system designed for the rapid removal of BigDye&trade; terminators, primers, and non-targeted amplicons to ensure reliable, high-resolution DNA readings.
</li>
<li dir="ltr" aria-level="1">
Scalable Purification Formats: Flexible volume options ranging from 1mL to 500mL, optimized for both manual workflows and seamless integration into fully automated liquid handling workstations for high-throughput sequencing labs.
</li>
</ul>
&nbsp;
<h2 data-section-id="109ahyv" data-start="1462" data-end="1499">How to choose a Sanger DTR product</h2>
&nbsp;
Cleanup chemistry
Gel-filtration cleanup remains one of the most commonly used approaches for Sanger sequencing purification. Products such as Performa DTR remove excess dye terminators, salts, nucleotides, and short primers without introducing additional chemicals that may affect downstream analysis. Optima DTR products use proprietary resin technology to achieve similar purification performance while offering additional format flexibility.
Processing format
Single-sample spin cartridges are suitable for laboratories performing occasional sequencing runs or troubleshooting individual samples.
Eight-well strip formats provide a practical solution for laboratories processing small to medium batches without requiring a full 96-well workflow.
Ninety-six-well plate formats are the preferred option for sequencing cores and high-throughput facilities because they allow simultaneous purification of large sample sets and direct transfer into sequencing plates.
Throughput requirements
Laboratories running a few sequencing reactions per week often prioritize convenience and flexibility. High-volume sequencing facilities generally select plate-based DTR systems that minimize handling time and support automated workflows.
Alternative cleanup methods
Ethanol precipitation is a low-cost cleanup method but requires multiple reagent additions, centrifugation steps, and careful sample handling. While effective, it is more labor-intensive and can introduce variability.
Bead-based systems such as BigDye XTerminator offer rapid purification with minimal hands-on time. These systems are often selected when workflow speed is more important than maximizing read length.
Read quality and read length
For laboratories focused on obtaining the longest possible sequence reads and minimizing retests, gel-filtration DTR methods remain the preferred choice. These systems consistently produce high-quality sequence traces with strong early-read performance and reduced dye contamination.
Chemistry compatibility
Most DTR products are compatible with multiple dye-terminator sequencing chemistries, including BigDye Terminator v1.1, BigDye Terminator v3.1, DYEnamic ET, WellRED terminators, and related sequencing reagents. This broad compatibility makes them suitable for both current and legacy sequencing workflows.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4992" data-end="5017">Specifications context</h2>
&nbsp;
Modern Sanger DTR products are designed for compatibility with major capillary electrophoresis platforms and sequencing chemistries. Gel-filtration systems typically use hydrated matrices that require no additional preservatives or buffer exchanges and allow purified sequencing products to be loaded directly into sequencing plates after centrifugation.
As of 2026, gel-filtration DTR cleanup in 96-well plate format remains the most widely adopted purification method in academic, clinical, and commercial Sanger sequencing facilities because of its strong performance in read length, sequence quality, and retest reduction. Bead-based purification systems continue to gain adoption in laboratories where rapid turnaround and high-throughput processing are primary workflow priorities.
Optimize your sequencing cleanup&mdash;&nbsp;<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">contact the MBP team</a> today for a quote on our professional HighPrep DTR solutions.

<h2>What are Sanger sequencing reagents?</h2>
&nbsp;
Sanger sequencing reagents are the chemical components used in chain-termination DNA sequencing, the method developed by Frederick Sanger in which a DNA polymerase extends a primer along a single-stranded template and is terminated at random positions by the incorporation of fluorescently labeled dideoxynucleotide terminators (ddNTPs) that lack the 3'-OH group needed for further elongation. The reaction produces a nested set of labeled fragments that, when resolved by capillary electrophoresis, gives the DNA sequence. Key reagents include dye-terminator mastermix (BigDye v3.1 or equivalent), template DNA, sequencing primer, and cleanup reagents.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/sequencing/sanger-sequencing/sanger-dtr" title="Sanger DTR (Dye Terminator Removal):">Sanger DTR (Dye Terminator Removal):</a> Effective purification systems such as Magbio HighPrep DTR employs paramagnetic bead technology to effectively eliminate surplus terminators and salts, guaranteeing clear baselines and extended read lengths.
</li>
</ul>
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/sequencing/sanger-sequencing/cycle-sequencing-kit" title="Cycle Sequencing Kits:">Cycle Sequencing Kits:</a> Durable formulations like the SmartDye Terminator 3.1, designed to deliver consistent peak heights and excellent processivity across various DNA templates, including plasmids and PCR products.
</li>
</ul>
&nbsp;
<h2>How to use Sanger sequencing reagents</h2>
&nbsp;
Clean up PCR products before sequencing reactions
Residual PCR primers and unincorporated dNTPs can interfere with the sequencing reaction by competing with the sequencing primer or being incorporated by the polymerase. ExoSAP enzymatic cleanup or column-based purification removes these contaminants before the sequencing reaction is set up.
Set up sequencing reactions with the minimum recommended cycle number
Sanger sequencing cycle sequencing reactions typically require 25&ndash;30 thermocycler cycles, fewer than a standard PCR, since only one primer is used and each cycle synthesizes a new set of terminated fragments rather than doubling the product. Using too many cycles can deplete the dye-terminator terminators and reduce signal quality.
Clean up the completed sequencing reaction before capillary electrophoresis
Unincorporated dye-terminator ddNTPs must be removed before capillary electrophoresis injection, since free fluorescent ddNTPs obscure the first 25&ndash;50 bases of the read. Column purification, ethanol-EDTA precipitation, or size-exclusion spin columns are standard cleanup approaches.
Use 10&ndash;50 ng of purified PCR product as template
Too little template produces weak, noisy signal; too much produces dye blobs from terminator over-incorporation. Typically 10&ndash;50 ng of PCR product or 200&ndash;500 ng of plasmid DNA is appropriate for a single sequencing reaction.
Position the sequencing primer at least 100 bp from the target region
Sanger reads have lower quality in the first 50&ndash;100 bases, with the high-quality window running from approximately base 100 to 900 from the primer. Placing the sequencing primer so that the target region falls within this window gives the best data quality.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
BigDye Terminator v3.1 has been the dominant commercial dye-terminator chemistry since its introduction, incorporating fluorescently labeled ddNTPs with distinct emission wavelengths for reads of consistently high quality up to approximately 1000 bp. As of 2026, Sanger sequencing remains the method of choice for mutation confirmation, plasmid verification, and colony screening, because its per-sample cost and turnaround for single loci is substantially more practical than NGS for these routine targeted applications.
Ensure the precision of your sequence&mdash;<a href="https://mbpinc.net/contact-us" title="reach out to the MBP team">reach out to the MBP team</a> now for a quote on our expert Sanger sequencing services.

Sanger Sequencing

HighPrep™ DTR - Protocol

Flyer

HighPrep™ DTR - MSDS

HighPrep DTR - DX IFU

Magbio HighPrep DTR

HighPrep DTR — DT-70000 by MagBio Genomics — is a magnetic bead reagent for Sanger sequencing reaction cleanup that removes dye-labeled terminators and salts from ABI and MegaBACE sequencing reactions in 96-well and 384-well formats. The Bind-Wash-Elute protocol uses 10 µL per sample in 96-well mode and 5 µL per sample in 384-well mode, with elution in low-salt buffer or nuclease-free water suitable for direct injection on capillary electrophoresis systems. Available from MBP in a 1–500 mL size range (DT-70001 to DT-70500) — place orders via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Main functions

Starting material (Sample matrix)

Starting amount

Process method

Purification method

Application

Elution Volume

HighPrep DTR (1 ml)

HighPrep DTR (5 ml)

HighPrep DTR (50 mL)

HighPrep DTR (250 mL)

HighPrep DTR (500 mL)

<h2>What Are Magnetic Bead HMW DNA Extraction Kits?</h2>
&nbsp;
A magnetic bead HMW DNA extraction kit uses Solid Phase Reversible Immobilization (SPRI)-type magnetic beads to bind genomic DNA released by gentle lysis, with a magnetic separator handling wash and elution steps in place of centrifugation, columns, or vacuum manifolds, producing fragment sizes in the 50-150 kb range depending on how beads are resuspended during elution. Related entities include PacBio Nanobind-type bead chemistry, KingFisher-compatible automated magnetic particle processors, A260/280 and A260/230 purity thresholds above 1.8, and downstream long-read sequencing library preparation for PacBio and Oxford Nanopore platforms. Choose magnetic bead HMW kits when fragment sizes in the 50-150 kb range meet your long-read sequencing needs, and you want to avoid centrifugation-based shearing.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Low-shear high molecular weight DNA isolation solutions using advanced paramagnetic bead technology, including the Magbio HighPrep HMW DNA Kit.</li>
<li>High-throughput magnetic bead-based extraction systems for simultaneous recovery of intact genomic DNA, including the Quick-DNA&trade; HMW Kit.</li>
<li>Workflow-friendly HMW DNA purification kits designed to preserve long DNA fragments for long-read sequencing applications.</li>
<li>Solutions compatible with manual and automated sample processing for efficient genomic DNA recovery.</li>
<li>HMW DNA extraction products suitable for genome assembly, structural variant analysis, and other advanced genomics workflows.</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead HMW DNA Kit</h2>
&nbsp;
Bead Resuspension Method and Fragment Size Tradeoff
Fragment size from magnetic bead HMW kits depends on how beads are resuspended after washing: gentle tube tapping typically yields fragments in the 50-150 kb range, while vortexing produces somewhat smaller fragments (around 40-100 kb) but may improve recovery consistency. If your sequencing application benefits from the largest possible fragments (e.g., resolving large structural variants), prioritize tapping-based resuspension protocols even if yield is marginally lower.
Sample Input and Expected Yield
Magnetic bead HMW kits for blood, cells, and tissue typically specify inputs such as 200 ul of fresh/frozen whole blood, 1 million cells, or up to 8 mg of tissue, with a minimum yield around 3 ug of HMW gDNA&mdash;matching the input requirements of PacBio library preparation (around 1 ug above 30 kb) and Oxford Nanopore ligation kits (100 ng to 1.5 ug). Confirm your sample type matches a documented protocol within the kit family rather than assuming a single universal protocol.
No Centrifuge, Column, or Vacuum Required
Unlike silica spin-column or 96-well plate HMW approaches, magnetic bead HMW kits eliminate centrifugation, columns, and vacuum manifolds entirely, relying only on a magnetic separator for all wash and elution steps. This simplifies equipment requirements for labs without access to specialized centrifuge rotors, though a magnetic stand or automated magnetic particle processor is still required.
Purity Ratios and RNA Removal
A260/280 and A260/230 ratios greater than 1.8 are generally preferred for HMW DNA from magnetic bead kits, with near-complete RNA removal reported as a feature of bead-based HMW chemistry. Ratios below this threshold can indicate protein or salt contamination requiring troubleshooting before proceeding to expensive long-read library preparation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The defining specifications for magnetic bead HMW DNA extraction are the fragment size achievable via the bead resuspension method chosen, sample-specific input requirements relative to the kit's documented minimum yield, and purity ratios above 1.8 for both A260/280 and A260/230. A kit yielding around 3 ug of DNA in the 50-150 kb range from 200 ul whole blood, 1 million cells, or up to 8 mg tissue meets standard PacBio and Nanopore library preparation inputs without centrifugation.&nbsp;
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Magbio HighPrep HMW DNA Kit

HighPrep HMW DNA Kit by MagBio Genomics is a magnetic bead-based system that isolates high molecular weight DNA (50–300+ kb) with A260/A280 and A260/A230 &gt;1.7 from blood, tissue, cells, and bacteria — yielding ≥10 µg from 10 mg tissue — for long-read sequencing, nanopore, microarray, and genotyping applications. Available in 5-prep (HPHMW-D5), 96-prep (HPHMW-D96), and 984-prep (HPHMW-D96x4) sizes.

Starting Material (Diverse Sample Types)

Process Method

Instrument

Purification Method

Automated Extraction on Kingfi sher™ Flex

Manual Extraction

Downstream Applications

HighPrep HMW DNA Kit (5 preps)

HighPrep HMW DNA Kit (96 preps)

HighPrep HMW DNA Kit (984 preps)

<h2>What are magnetic bead genomic DNA kits for insects?</h2>
&nbsp;
A magnetic bead genomic DNA kit for insects uses paramagnetic beads to bind DNA released by a lysis and proteinase K digestion step from insect-derived material ranging from a single leg or wing to a whole body, with a magnetic separator handling wash and elution steps instead of centrifugation, processing up to 96 samples in under an hour manually or via automation. Related entities include deep-well plate sample loading, MAG-S1-type magnetic particles, manual or automated magnetic separation devices, and downstream PCR, qPCR, cloning, and sequencing applications. Choose magnetic bead kits for insect genomic DNA when working from minimal tissue input (single leg or wing) or building an automated extraction pipeline for arthropod samples.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Automated insect genomic DNA isolation using Silica-coated Mag Beads for</li>
<li dir="ltr" role="presentation">Maximum yield from small invertebrates using high-binding arthropod magnetic particles&nbsp;</li>
<li dir="ltr" role="presentation">Compatible Automated insect DNA reagents with high-throughput liquid handlers</li>
<li dir="ltr" role="presentation">Walk-away invertebrate genomic DNA purification using Functionalized Mag Beads&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a magnetic bead insect genomic DNA kit</h2>
&nbsp;
Tissue input flexibility
Magnetic bead insect kits are documented to work from a single leg or wing up to a whole insect body, across species including mosquitoes, flies, and ants, with samples loaded into deep-well plates and brought to volume with a buffer before bead-based binding. This flexibility is useful for non-destructive or minimally destructive sampling protocols where preserving the rest of the specimen (e.g., for morphological identification) is important.
Manual vs. automated throughput
Up to 96 samples of insect legs or wings can be processed in less than an hour using magnetic bead chemistry, either manually with a magnetic stand or via automated extraction systems. If your lab already runs an automated nucleic acid extraction system for other sample types, confirm the bead chemistry and plate format are compatible before adopting for insect samples.
Storage and handling of kit components
Magnetic bead particles and proteinase K solutions in insect kits commonly require refrigerated storage (2-8 degrees Celsius), while other buffer components are stored at room temperature; extracted DNA is generally stored at -20 degrees Celsius. Plan reagent storage space accordingly, especially for kits ordered in bulk for large specimen collections.
Downstream application range
DNA from magnetic bead insect kits is documented as suitable for PCR, qPCR, cloning, sequencing, and other molecular biology applications, with high yields and quality reported across the input range from single legs to whole bodies. For applications requiring higher molecular weight DNA from insects, confirm whether the kit's chemistry is optimized for HMW output or standard fragment sizes.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The specs that most differentiate magnetic bead insect kits from silica plate or spin-column alternatives are minimum tissue input (down to a single leg or wing), processing time without centrifugation (under an hour for 96 samples), and storage requirements for bead and enzyme components. A kit that processes a single leg through to a whole body with the same chemistry gives flexibility for both non-destructive and destructive sampling protocols in the same lab. Labs running automated extraction pipelines for population genetics often pair this category with <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect/96-well-plate">96-well plate insect kits</a>&nbsp;during validation, while labs processing individual specimens for method development typically start with&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect/spin-column">insect spin-column kits</a>. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA extraction kits category</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;link to related formats, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue/mag-beads">blood/tissue mag bead kits</a> use comparable bead chemistry for animal samples.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

HighPrep™ Insect DNA Kit - Protocol

Magbio HighPrep Insect DNA Kit

HighPrep Insect DNA Kit by MagBio Genomics extracts PCR-quality genomic DNA from insect legs, wings, and whole organisms (mosquitoes, flies, ants, arthropods) using MAG-S1 paramagnetic beads; 8-step organic solvent-free protocol, 96 preps in under 1 hour, 50&ndash;200 &micro;L elution, 14-month shelf life. Available in 5 preps (HPI-D5), 96 preps (HPI-D96), and 384 preps (HPI-D96x4) from MBP INC.

Storage

Stability

HighPrep Insect DNA Kit  (5 preps)

HighPrep Insect DNA Kit  (96 preps)

HighPrep Insect DNA Kit (384 preps)

DNA/PCR

<h2>What is DNA/PCR Sequencing Cleanup?</h2>
&nbsp;
DNA/PCR sequencing cleanup purifies PCR-amplified NGS library products - indexed Illumina libraries after amplification, amplicon sequencing PCR products (16S rRNA, ITS, targeted gene panels), and long-range PCR products for long-read sequencing - removing residual PCR primers, dNTPs, thermostable polymerase, betaine, DMSO, BSA, and PCR buffer salts that inhibit sequencing cluster generation, flow cell loading, and library quantification. Perform SPRI bead cleanup after every PCR amplification step in library preparation - residual primers generate non-informative sequencing reads, betaine carryover inhibits cluster generation chemistry, and thermostable polymerase coats flow cell surfaces, reducing cluster density and data yield.
&nbsp;
<h2>What you will find</h2>
&nbsp;
<ul>
<li>Magbio HighPrep&trade; PCR: A highly flexible, bead-based reagent that simplifies post-PCR cleanup and dual-sided size selection, fitting seamlessly into your high-throughput automated platforms.</li>
<li>HighPrep&trade; PCR PB Kit: Specifically optimized for the rigorous demands of Pacific Biosciences (PacBio) workflows, this kit ensures you recover the long-read fragments your research depends on.</li>
<li>Selective Contaminant Depletion: Our buffers are engineered to remove salts and polymerases without the stress of centrifugation, delivering transfection-grade purity in every sample.</li>
</ul>
&nbsp;
<h2>How to Choose a DNA/PCR Sequencing Cleanup Reagent</h2>
&nbsp;
Bead Ratio for Target Amplicon Size
The bead-to-sample ratio determines the minimum fragment size retained. At 0.8x: retains &gt;=300 bp; removes primers and adapter dimers; best for amplicons &gt;=300 bp (16S V3-V4, ITS2). At 0.9x: retains &gt;=200 bp; removes primers and adapter dimers; standard for indexed library amplicons. At 1.0x: retains &gt;=150 bp; removes primers; useful for short-insert libraries (100-200 bp). At 1.8x: retains &gt;=100 bp; maximum primer removal; over-cleaning for library amplicons. Always calibrate the bead ratio using a test library at your target amplicon size before processing study samples; empirical calibration is required because bead lot performance and PEG concentrations vary.
Double-Side SPRI After PCR
For amplicon libraries where the PCR product range is wide (multiple bands) or where large amplification artifacts must be removed alongside primers, double-sided SPRI after PCR selects the target amplicon precisely: the first step removes amplification artifacts above the target size; the second step retains the target amplicon and discards primers and primer dimers.
Bead-Based vs. Spin Column After PCR
AMPure XP beads are preferred for high-throughput 96-well plate PCR cleanup in amplicon sequencing workflows where 96-384 individual PCR amplicons must be cleaned before pooling. Spin columns (Zymo DNA Clean &amp; Concentrator-5) are used for small-batch amplicon cleanup when concentrated eluate in 6-30 &micro;l is needed before capillary electrophoresis or other downstream analysis.
Betaine and DMSO Removal
If your PCR protocol includes betaine (1-2 M) or DMSO (1-5%), confirm complete removal by checking that the post-cleanup DNA solution is free of unusual viscosity (indicating residual betaine). At the standard bead ratio, betaine and DMSO are completely removed during the 2x 80% ethanol wash steps. If betaine or DMSO carryover is suspected (reduced sequencing cluster density), add 200 &micro;l ethanol wash before elution.
Amplicon Sequencing Pool Cleanup
After individually cleaning and quantifying each amplicon library by Qubit HS, pool at equimolar ratios before the final SPRI cleanup of the pool. Final pool cleanup at 0.9x removes any residual primer from individual library cleanup steps and normalizes the final pool for sequencing loading.
&nbsp;
<h2>Specifications Context</h2>
HighPrep&trade; PCR uses SPRI magnetic bead technology for post-PCR cleanup and DNA size selection. Adjustable bead-to-sample ratios enable efficient removal of primers, primer dimers, adapter dimers, nucleotides, enzymes, and buffer components while selectively retaining target DNA fragments. Standard ratios support PCR product cleanup, while dual-sided selections provide precise library size distribution for NGS workflows. HighPrep&trade; PCR PB Kit is optimized for PacBio sequencing, maximizing recovery of long DNA fragments while depleting smaller contaminants. The centrifugation-free workflow supports both manual and automated processing and is compatible with Illumina, ONT, and PacBio library preparation.
&nbsp;
Ready to refine your library? The MBP team is here to help&mdash;<a href="https://mbpinc.net/contact-us">contact us</a> today for a personalized quote on our PCR size selection reagents.

Magbio HighPrep PCR

HighPrep PCR by MagBio Genomics is a paramagnetic bead reagent for post-PCR cleanup and NGS-ready DNA size selection, retaining fragments ≥100 bp at 1.8× ratio, with >70% DNA recovery, ≥15 µL elution, 18-month shelf life at 2–8°C, and KingFisher Flex automation compatibility. Available in 1mL (AC-60001), 5mL (AC-60005), 50mL (AC-60050), 250mL (AC-60250), and 500mL (AC-60500).

RNA recovery

RNA Purity

HighPrep PCR (1 mL)

HighPrep PCR (5 mL)

HighPrep PCR (50 mL)

HighPrep PCR (250 mL)

HighPrep PCR (500 mL)

<h2>What are Magnetic Bead Genomic DNA Kits for Plant Tissue?</h2>
&nbsp;
A magnetic bead genomic DNA kit for plant tissue uses paramagnetic beads to bind genomic DNA released from lysed plant cells, while a magnetic separator holds the beads in place during wash and elution steps. This approach eliminates the need for centrifugation, vacuum manifolds, and silica columns that can become problematic when processing polysaccharide-rich plant lysates. Related workflows include automated magnetic particle processing, SPRI-based DNA purification, and downstream PCR, qPCR, genotyping, and sequencing applications. Choose magnetic bead kits for plant genomic DNA when working with difficult plant tissues or when building an automated&nbsp;extraction workflow.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Automated plant genomic DNA isolation using silica-coated magnetic beads for high-throughput extraction workflows.</li>
<li>High-binding plant magnetic particles designed to maximize DNA recovery from a wide range of plant specimens.</li>
<li>Paramagnetic bead cleanup reagents for post-extraction removal of polyphenols and other contaminants.</li>
<li>Plant DNA extraction reagents compatible with automated liquid handling systems and magnetic particle processors.</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Plant Genomic DNA Kit</h2>
&nbsp;
Sample Input and Throughput
Magnetic bead plant DNA kits commonly process up to 96 samples of approximately 50 mg fresh tissue or 15 mg dried tissue per sample in under one hour. Confirm that your typical sample input falls within the recommended range to achieve consistent DNA yield and purity.
Clogging-Prone Tissue Types
Fibrous plant tissues and samples rich in polysaccharides can challenge traditional column-based extraction methods. Magnetic bead chemistry binds DNA directly in solution, reducing issues associated with clogged filters and inconsistent flow rates.
Manual Versus Automated Processing
Magnetic bead kits can be processed manually using a magnetic stand or integrated into automated magnetic particle processors for higher throughput. Laboratories already using automated extraction systems should verify compatibility with existing instruments and plate formats.
Standard Versus High-Molecular-Weight DNA
Some magnetic bead kits are optimized for high-molecular-weight DNA suitable for long-read sequencing and genome assembly applications, while standard formats are designed for PCR, qPCR, and routine genotyping workflows.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The key specifications that distinguish magnetic bead plant DNA kits are processing speed, automation compatibility, resistance to clogging from plant debris, and DNA fragment size output. Many workflows can process up to 96 samples in under one hour without centrifugation or vacuum filtration, making them attractive for high-throughput laboratories.&nbsp;Laboratories working with fibrous, polysaccharide-rich, or otherwise challenging plant tissues often evaluate magnetic bead chemistry when column-based methods prove inefficient. The format is also widely adopted in automated workflows due to its compatibility with liquid handling systems and magnetic particle processors.&nbsp;
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the specialist team</a> for guidance on magnetic bead chemistry selection, automation platform compatibility, and workflow optimization.

Protocol

Magbio HighPrep Plant DNA Plus Kit

HighPrep Plant DNA Plus Kit (HPPP-D0) by MagBio Genomics — magnetic bead-based plant DNA extraction without grinding. MAG-D1 particles deliver A260/A280 &ge;1.8, fragments &gt;50 kb. Compatible with PCR, NGS, Southern blotting, and cloning. Manual or automated processing. Available in 5 (HPPP-D5), 50 (HPPP-D50), 100 (HPPP-D100), and 400 (HPPP-D100x4) prep sizes.

Sample amount (Input)

Sample sources

Instrument/Equipment

HighPrep Plant DNA Plus Kit (5 Preps)

HighPrep Plant DNA Plus Kit (50 Preps)

HighPrep Plant DNA Plus Kit (100 Preps)

HighPrep Plant DNA Plus Kit (400 Preps)

<h2>What are Magnetic Bead Plant RNA Extraction Kits?</h2>
&nbsp;
Magnetic bead plant RNA extraction kits purify total RNA from plant tissue -- leaves, stems, roots, seeds, and other plant organs -- using mechanical disruption in a specialized plant lysis buffer followed by binding of RNA to paramagnetic silica-coated beads, magnetic separation during successive wash steps to remove polyphenols, polysaccharides, and other plant-specific inhibitors, and elution in RNase-free water without centrifugation for the RNA purification phase. The 96-well format processes all samples simultaneously on liquid-handling robots (KingFisher Flex, Tecan Fluent, Hamilton STAR), enabling the scale needed for plant breeding programs, genome-wide association studies (GWAS) linked to expression, and abiotic stress response screens, processing hundreds of individual plant samples per day. Choose magnetic bead plant RNA extraction when processing more than 48 plant samples per session, when automation infrastructure is available, or when high-polysaccharide lysates cause consistent column clogging in spin-column formats.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="bjjl4q" data-start="25" data-end="128">Magbio HighPrep Plant RNA Plus Kit for rapid isolation of total RNA from complex plant tissues.</li>
<li data-section-id="1r07f8r" data-start="129" data-end="191">Magnetic bead&ndash;based purification for efficient RNA recovery.</li>
<li data-section-id="s295jn" data-start="192" data-end="258">Automation-ready workflow for high-throughput sample processing.</li>
<li data-section-id="6vw6z8" data-start="259" data-end="318">Effective extraction from challenging plant sample types.</li>
<li data-section-id="1u9s43l" data-start="319" data-end="399" data-is-last-node="">High-quality total RNA suitable for downstream molecular biology applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Plant RNA Kit</h2>
&nbsp;
Automation Platform and Script Availability
Confirm the kit manufacturer provides validated automation scripts for your instrument before purchase. Thermo Fisher MagMAX Plant RNA Isolation Kit provides KingFisher Flex and Apex scripts; Zymo Direct-zol-96 MagBead supports Tecan and Hamilton in addition to KingFisher. For other platforms, contact MBP's technical team to confirm script availability or the feasibility of in-house protocol adaptation.
CTAB vs. Guanidinium Lysis
Guanidinium-based lysis buffers are compatible with most magnetic bead RNA kits and are the default for standard plant species. For high-polyphenol species (Vitis, Pinus, Citrus) where guanidinium lysis produces brown, inhibitor-rich lysates, CTAB-based upstream lysis can be adapted for magnetic bead purification by adjusting the lysate-to-ethanol ratio before bead addition; confirm protocol compatibility with the specific kit before attempting CTAB lysis with magnetic bead formats.
Tissue Disruption Before Bead Extraction
Magnetic bead kits do not include mechanical disruption; plant tissue must be ground in liquid nitrogen to a fine powder or bead-beaten in lysis buffer before adding the lysate to the bead extraction plate. For 96-sample throughput, a 96-well bead mill system paired with a 96-deep-well plate is the most efficient tissue disruption setup for plant material at scale.
Small RNA Retention
Confirm the kit retains RNAs &gt;=17 nt from plant tissue, including miRNAs, ta-siRNAs, and siRNAs. Silica bead formats generally retain the full RNA size spectrum; check the kit datasheet for the minimum RNA size retained.
Post-Extraction Quality Assessment
For large batch processing, run a representative subset (1 plate per 5 batches) on a Bioanalyzer RNA Nano chip to monitor RIN values over time. Declining RIN values across batches signal RNase contamination of reagents or incomplete tissue disruption that reduces RNA integrity before bead binding.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Quality targets for magnetic bead plant RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, RIN &gt;= 7, and yield of 1-20 ug from 50 mg leaf tissue. For large-scale RNA-seq (&gt;48 samples per run), RNA-seq library preparation kits validated for automated input (Zymo-Seq RiboFree Plant) are required downstream. High-throughput plant RNA-seq for quantitative trait loci (eQTL) studies and genomic selection in crop improvement programs at plant research institutes regularly processes 96-well batches of plant RNA, with magnetic bead extraction enabling the scale these programs require.&nbsp;
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc. </a>to get optimized mag bead systems for high-throughput workflows.

HighPrep Plant RNA Plus Kit - Protocol

Magbio HighPrep Plant RNA Plus Kit

HighPrep Plant RNA Plus Kit by MagBio Genomics purifies RNA from 10&ndash;50 mg of plant tissue or 10&ndash;20 mg of seeds using MAG-R5 magnetic beads; A260/A280 &ge;1.8; integrated DNase I digestion (37&deg;C, 15 min); removes polysaccharides and phenolics; KingFisher&trade; Flex compatible. Available in 10 preps (HPPP-R10), 96 preps (HPPP-R96), and 384 preps (HPPP-R96x4) from MBP INC.

Sample Source

HighPrep Plant RNA Plus Kit (10 Preps)

HighPrep Plant RNA Plus Kit (96 Preps)

HighPrep Plant RNA Plus Kit (384 Preps)

MagBead

<h2>What Are Magnetic Bead Plasmid DNA Extraction Kits?</h2>
&nbsp;
Magnetic bead plasmid DNA kits use superparamagnetic nanoparticles, typically silica-coated or anion-exchange-functionalized, suspended in optimized binding buffers to capture plasmid DNA from bacterial lysate. After alkaline lysis of E. coli culture (0.5-5 mL per reaction), the beads bind DNA; an external magnetic field then immobilizes the beads so supernatant, RNA, and proteins are washed away without centrifugation. The purified DNA is eluted in water or low-salt buffer and is ready for downstream use. Throughput scales from single-tube manual processing to 96-well plate automation on KingFisher Duo, MagnetaPure 32, or ASSIST PLUS liquid handlers. Choose magnetic bead kits when your lab runs plasmid minipreps at scale (24+ samples/run) or when centrifuge-free automation is required.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Magbio HighPrep Plasmid DNA Plus Kit for rapid, automated plasmid purification using high-binding paramagnetic particle technology.</li>
<li>Zyppy&trade;-96 Plasmid MagPrep Kit for scalable magnetic separation of high-purity vector constructs.</li>
<li>Zyppy-96 Plasmid MagBead Prep Kit for faster, column-free plasmid isolation.</li>
<li>Magnetic bead-based plasmid purification solutions compatible with high-throughput laboratory workflows.</li>
<li>Column-free plasmid DNA extraction systems that simplify processing and reduce hands-on time.</li>
<li>Products designed for efficient recovery of clean plasmid DNA for cloning, sequencing, and transfection applications.</li>
<li>Automation-friendly plasmid preparation kits suitable for 96-well and large-batch sample processing.</li>
</ul>
&nbsp;
<h2>How to Choose Magnetic Bead Plasmid Kits</h2>
&nbsp;
Automation Platform Compatibility
Bead-based kits are designed for specific magnetic rod or liquid-handler platforms. Confirm compatibility before ordering: NucleoMag Plasmid kits are validated for MagnetaPure and IsoPure Mini; MagJET kits are optimized for the KingFisher series; MagMAX Pro HT kits are designed for high-throughput centrifuge-free workflows. Using a kit outside its validated platform may require buffer volume adjustments and is not supported by the manufacturer.
Bead Type and DNA Binding Capacity
Silica-coated magnetic beads use chaotropic binding chemistry similar to spin columns; anion-exchange-functionalized beads offer higher selectivity for covalently closed circular (CCC/supercoiled) plasmid. Standard miniprep bead kits bind 5-20 ug from 1.5 mL culture; scaled protocols for 5 mL can yield up to 50 ug. Check binding capacity against your expected yield to avoid saturation.
High-Copy vs Low-Copy Plasmid
Most bead-based kits are validated for standard high-copy plasmids (pUC, pBR322 derivatives). Low-copy constructs such as fosmids or BACs require longer lysis times and may need protocol modifications; check the kit's validated plasmid types before purchasing for non-standard vectors.
Elution Volume and Concentration
Bead protocols typically elute in 50-100 uL. If downstream transfection requires high DNA concentration, choose kits that support low-volume elution (50 uL) or perform a secondary concentration step. Check that the eluate is compatible with your downstream assay buffer conditions.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Plasmid DNA purity from bead-based kits is evaluated by the A260/A280 ratio (target 1.8-2.0) and the A260/A230 ratio (&gt;1.8). Supercoiling percentage (measured by agarose gel densitometry) should exceed 80% for standard transfection applications. Automated 96-well bead workflows complete plasmid isolation in 30-60 minutes for a full plate, compared to 1-2 hours for centrifuge-based spin-column processing of the same number of samples. All kits are Research Use Only (RUO).
&nbsp;
<section class="mbp-contact-cta"></section>
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.

Magbio HighPrep Plasmid DNA Plus Kit

HighPrep Plasmid DNA Plus Kit by MagBio Genomics purifies plasmid DNA from 1.0&ndash;1.5 mL E. coli cultures using magnetic bead technology; high-copy yield ~10&ndash;15 &micro;g, 50 &micro;L elution, &lt;60 min for 96 preps; supports plasmids, BACs, PACs, cosmids, and fosmids; KingFisher&trade; Flex compatible. Available in 5 (HPPL-DP5), 96 (HPPL-DP96), 384 (HPPL-DP96x4), 1,920 (HPPL-DP96x20), and 3,840 preps (HPPL-DP96x40) from MBP INC.

Lysate clearance

HighPrep Plasmid DNA Plus Kit (5 Preps)

HighPrep Plasmid DNA Plus Kit (96 preps)

HighPrep Plasmid DNA Plus Kit (384 preps)

HighPrep Plasmid DNA Plus Kit (1920 preps)

HighPrep Plasmid DNA Plus Kit (3840 preps)

RNA/c-DNA

<h2>What are RNA/cDNA Sequencing Cleanup Reagents?</h2>
&nbsp;
RNA/cDNA sequencing cleanup reagents purify RNA and complementary DNA during RNA sequencing library preparation workflows by removing enzymes, buffer salts, unincorporated nucleotides, short nucleic acid fragments, and other reaction contaminants that can interfere with downstream processing and sequencing. These reagents are commonly used after RNA processing, cDNA synthesis, library construction, amplification, and other enzymatic steps to improve nucleic acid purity and workflow performance.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Magbio HighPrep&trade; RNA Elite: A high-performance magnetic reagent optimized for the selective recovery of RNA and cDNA from complex enzymatic reactions.</li>
<li>RNA Purification Magnetic Beads: High-affinity matrices engineered for rapid, centrifugation-free isolation, delivering reproducibility-tested yields in automated workflows.</li>
</ul>
&nbsp;
<h2>How to Choose an RNA/cDNA Sequencing Cleanup Reagent</h2>
&nbsp;
RNA vs. cDNA Workflows
Select a cleanup reagent compatible with the nucleic acid being processed. RNA-focused cleanup reagents are designed for purification and recovery of RNA products, while many magnetic bead-based systems can also be used for cDNA cleanup, concentration, and size-selection workflows.
Cleanup vs. Size Selection
Cleanup workflows remove reaction contaminants such as enzymes, salts, and unincorporated nucleotides. Size-selection workflows enrich RNA or cDNA fragments within a desired size range when required by the downstream library preparation protocol.
Throughput Requirements
Magnetic bead-based cleanup systems support both low-throughput manual processing and higher-throughput workflows that benefit from automation and 96-well format compatibility.
Automation Compatibility
Magnetic bead-based purification eliminates centrifugation during the cleanup process and can be integrated into automated liquid-handling and magnetic separation workflows.
Quality Assessment
Following cleanup, RNA and cDNA quality are commonly assessed using fluorometric quantification methods and fragment analysis platforms to verify concentration, integrity, and size distribution prior to sequencing.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
RNA/cDNA sequencing cleanup reagents provide purification of RNA and cDNA products generated during library preparation and other enzymatic workflows. Magnetic bead-based systems enable cleanup, concentration, and size selection without organic extraction or vacuum filtration and are compatible with both manual and automated processing.&nbsp;MagBio HighPrep RNA Elite supports the cleanup and recovery of RNA and cDNA products used in transcriptomics and sequencing workflows, while RNA Purification Magnetic Beads provide rapid magnetic bead-based purification and recovery of RNA samples without centrifugation.&nbsp;These reagents are widely used in RNA sequencing, transcriptomics, gene expression analysis, and related molecular biology workflows where contaminant removal, nucleic acid recovery, and workflow scalability are important for library quality and downstream sequencing performance.
&nbsp;
Ensure the fidelity of your molecular data&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our advanced RNA/cDNA cleanup solutions.

HighPrep RNA Elite - MSDS

HighPrep RNA Elite - DX IFU

HighPrep RNA Elite

Magbio HighPrep RNA Elite

HighPrep RNA Elite by MagBio Genomics is a paramagnetic bead reagent for manual and automated purification of miRNA, siRNA, aRNA, total RNA, and single-stranded cDNA, compatible with 96-well and 384-well formats; no centrifugation required, 14-month shelf life at 4&ndash;8&deg;C. Available in 1 mL (RC-90001), 5 mL (RC-90005), 50 mL (RC-90050), 250 mL (RC-90250), and 500 mL (RC-90500) from MBP INC.

No. of reactions in 96 well format

No.of reactions In 384 Well Format

HighPrep RNA Elite (1 mL)

HighPrep RNA Elite (5 mL)

HighPrep RNA Elite (50 mL)

HighPrep RNA Elite (250 mL)

HighPrep RNA Elite (500 mL)

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
&nbsp;
Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
&nbsp;
Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Protocol - HighPrep™ Total RNA Isolation Kit - iSWAB™

Magbio HighPrep Total RNA Isolation Kit-iSWAB™

HighPrep Total RNA Isolation Kit-iSWAB (ISTOR-R0) by MagBio Genomics — magnetic bead-based RNA extraction from 600 µL buccal swab lysate (MAWI iSWAB-RNA V2). Elutes 30–50 µL, OD260/280 1.8–2.0, DNase I included. RT-qPCR, RNA-seq, cDNA synthesis compatible. Available in 5 (ISTOR-R5), 50 (ISTOR-R50), 100 (ISTOR-R100), and 400 (ISTOR-R100x4) prep sizes.

HighPrep Total RNA Isolation Kit - iSWAB™ (5 preps)

HighPrep Total RNA Isolation Kit - iSWAB™ (50 preps)

HighPrep Total RNA  Isolation Kit - iSWAB™ (100 preps)

HighPrep Total RNA  Isolation Kit - iSWAB™  (400 preps)

HighPrep™ Total RNA Plus Kit - Protocol

DataSheet

Magbio HighPrep Total RNA Plus Kit

HighPrep Total RNA Plus Kit by MagBio Genomics is a magnetic bead-based total RNA extraction system for swab lysates, tissue (~30&nbsp;mg), whole blood (200&nbsp;&micro;L), and cultured cells (1&times;106); 7-step protocol with on-bead DNase treatment, OD260/280 purity 1.9&ndash;2.0, &ge;30&nbsp;&micro;L elution, KingFisher Flex compatible. Available in 5 preps (HPTOR-R5), 50 preps (HPTOR-R50), 100 preps (HPTOR-R100), and 400 preps (HPTOR-R100x4) from MBP INC.

Downstream application

HighPrep Total RNA Plus Kit (5 preps)

HighPrepTotal RNA Plus Kit (50 preps)

HighPrep Total RNA Plus Kit (100 preps)

HighPrep Total RNA Plus Kit (400 preps)

<h2>What are Magnetic Bead Viral DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Magnetic bead viral DNA/RNA co-extraction kits purify both viral DNA and RNA from plasma, serum, urine, nasopharyngeal swabs, saliva, blood, cellular suspensions, biopsies, fecal samples, and cell culture media stored in DNA/RNA Shield using paramagnetic silica-coated beads that bind nucleic acids from &gt;50 nt to &gt;200 kb during a single chaotropic lysis step, with magnetic separation during successive wash steps removing inhibitors - no centrifugation required during purification. The 96-well format integrates with KingFisher Flex, Tecan Fluent, and Hamilton STAR liquid-handling platforms, enabling fully automated extraction of both RNA viruses (SARS-CoV-2, influenza, HIV) and DNA viruses (adenovirus, herpesvirus, parvovirus) from 96 samples per run. Choose the magnetic bead format for viral DNA/RNA co-extraction when processing 48+ samples per run, when a compatible automation platform is available, or when the program requires EUA-associated workflows validated for SARS-CoV-2 or other respiratory panel targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Magbio HighPrep Viral-Bacterial DNA &amp; RNA Kit (HVB-DR0): Scalable magnetic bead chemistry optimized for the simultaneous extraction of viral and bacterial nucleic acids from diverse fluid profiles.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral DNA &amp; RNA Kit (HPV-DR0): High-efficiency magnetic isolation systems engineered for the rapid purification of viral target vectors from fluid specimens.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Magbio HighPrep Viral-Pathogen DNA &amp; RNA Kit (HPP-DR0): Specialized bead technology designed to maximize pathogen recovery and eliminate inhibitors from challenging biological matrices.
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Quick-DNA/RNA Viral MagBead (R2140): Advanced magnetic-bead separation configurations built for fast, high-purity co-purification of total viral nucleic acids.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Viral DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Automation Platform Compatibility
Confirm the kit's automation scripts are available for your specific platform before purchase. The Quick-DNA/RNA Viral MagBead Kit is used in Zymo Research's SARS-CoV-2 EUA workflow and is compatible with KingFisher and other major liquid-handling platforms; contact MBP's technical team or Zymo Research's automation support for current script availability. For Tecan Fluent or Hamilton STAR, platform-specific script development or adaptation may be required.
DNA/RNA Shield as the Collection Medium
The Quick-DNA/RNA Viral MagBead Kit is designed around DNA/RNA Shield as the sample collection, inactivation, and storage medium. Swabs or other samples stabilized in DNA/RNA Shield load directly into the extraction protocol without additional neutralization steps. For programs using VTM (viral transport medium), UTM, or PBS as collection media, confirm compatibility with the kit's lysis buffer by testing spike-in recovery before adopting at scale.
Carrier RNA Requirement
Unlike the QIAGEN QIAamp Viral RNA Mini Kit, the Quick-DNA/RNA Viral MagBead Kit does not require the user to prepare and add carrier RNA to the lysis buffer - the kit's buffer system is formulated for complete lysis and efficient nucleic acid binding without this step. Eliminating the carrier RNA preparation step reduces per-batch setup time and removes one source of inter-batch variability in high-throughput automated workflows.
Nucleic Acid Size Recovery Range
Confirm the kit recovers the size range needed for your downstream application. The Quick-DNA/RNA Viral MagBead Kit binds nucleic acids from &gt;50 nt to &gt;200 kb - covering small viral RNAs (miRNA-sized fragments from viral genomes), standard RT-qPCR amplicon targets, and full-length viral genomes for metagenomic NGS. Applications requiring recovery of very short fragments (&lt;50 nt) need a kit specifically validated for that range.
Regulatory Requirements
For IVD diagnostic use or regulatory submissions, confirm the kit's current regulatory status. The Quick-DNA/RNA Viral MagBead Kit has been used in EUA workflows; its current authorizations and intended uses may have evolved since initial authorization. Always verify directly with Zymo Research or MBP's regulatory affairs team for current compliance documentation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The Quick-DNA/RNA Viral MagBead Kit binds small (&gt;50 nt) and large (&gt;200 kb) DNA and RNA to magnetic beads and elutes high-quality nucleic acids ready for NGS, hybridization-based detection, and RT-qPCR. The kit is used in Zymo Research's SARS-CoV-2 EUA extraction workflow and has been adopted in published multiplex respiratory panel methods and pan-pathogen metagenomics protocols. A published PMC study on integrated DNA and RNA extraction from viral pathogens causing acute respiratory infections demonstrated that magnetic bead-based co-extraction achieved equivalent or superior sensitivity to spin-column formats for both RSV (RNA virus) and adenovirus (DNA virus) from the same nasopharyngeal swab input. Magnetic bead viral co-extraction is the standard format for large public health reference laboratories and clinical metagenomics programs processing 96-1,500+ samples per day across outbreak response and surveillance networks.
&nbsp;
Contact the helpful <a href="https://mbpinc.net/contact-us">team at MBP</a> today to request a quote for your specific research requirements.

Viral DNA/RNA Kit - Performance Data

Viral DNA/RNA Kit - Sensitivity

Comparative Data - How do we fare against key competitors?

Magbio HighPrep Viral DNA & RNA Kit

HighPrep Viral DNA &amp; RNA Kit — HPV-DR0 by MagBio Genomics — isolates total viral nucleic acids (DNA and RNA) from whole blood, serum, plasma, saliva, nasopharyngeal swabs, and other body fluids using MAG-S1 magnetic beads, with 200–400 µL input and 30–100 µL elution in nuclease-free water. Optimized for SARS-CoV-2 RNA and compatible with KingFisher Flex automation (23 min) and other liquid handling workstations; no organic solvents required. Available from MBP in 5 (HPV-DR5), 96 (HPV-DR96), and 384 (HPV-DR96x4) prep sizes — order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.