Globe Scientific

Syringe Filters

<h2 dir="ltr">What are Syringe Filters?</h2>
 Syringe filters are self-contained, disposable filtration units comprising a polypropylene (PP) housing enclosing a flat-sheet membrane, with a female Luer lock inlet and a male Luer lock outlet. They are attached to a standard syringe, the sample is drawn up, and pushed through the membrane by manual plunger pressure. Standard disc diameters are 4 mm, 13 mm, 25 mm, and 33 mm; membrane pore sizes are 0.22 &micro;m (sterilizing grade) and 0.45 &micro;m (clarification grade), with 0.10 &micro;m available for mycoplasma removal. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 25 mm filter &asymp; 2.98 cm&sup2;. Globe Scientific syringe filters, a confirmed MBP brand, are available in PP housing with PES, PVDF, CA (cellulose acetate), MCE, and nylon membranes in 13 mm and 30 mm diameters, sterile (individually wrapped) and non-sterile (bulk), 0.22 &micro;m and 0.45 &micro;m. Choose syringe filters for volumes of 1&ndash;150 mL; choose vacuum bottle-top filters for volumes above 150 mL.
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<h2 dir="ltr">What you will find:</h2>
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PES Syringe Filters for high-flow aqueous filtration
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PVDF Syringe Filters for low protein binding
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Nylon Syringe Filters for organic solvent compatibility
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0.22&mu;m Syringe Filters for absolute sterilization
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0.45&mu;m Syringe Filters for clarification and pre-filtration
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PTFE Syringe Filters for aggressive chemical resistance
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Hydrophilic Syringe Filters for versatile liquid handling
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Sterile Individually Blistered Filters for aseptic security
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<h2 dir="ltr"> How to Choose Syringe Filters</h2>
 Diameter Selection by Sample Volume 13 mm syringe filters (effective area 0.92 cm&sup2;) handle up to 10&ndash;15 mL per filter before flow resistance becomes impractical. Use with 1&ndash;10 mL syringes. 25 mm syringe filters (2.98 cm&sup2;) handle 10&ndash;100 mL per filter. Use with 10&ndash;60 mL syringes. 33 mm syringe filters (approximately 5&ndash;6 cm&sup2;) handle up to 150 mL and are the largest practical syringe filter format. For volumes above 150 mL, switch to a vacuum bottle-top filter system. Matching filter diameter to sample volume prevents premature membrane clogging and excessive plunger force. Membrane Chemistry for Syringe Filters PES (Polyethersulfone): Low protein binding, high flow rate, aqueous compatibility. Standard choice for biological sample sterilization, cell culture supplement filtration (antibiotics, glutamine, growth factors), and conditioned medium aliquots. Not suitable for strong organic solvents or concentrated acids. PVDF (Polyvinylidene fluoride, hydrophilic): Low protein binding &mdash; among the lowest of all syringe filter membranes, with protein recovery rates &gt;90&ndash;99.9% documented for antibody and enzyme solutions. Compatible with mild organic solvents, weak acids, and most aqueous solutions. The first choice when filtering precious protein solutions (antibodies, enzymes, viral preparations) is where maximum protein recovery is critical. Not suitable for pure DMSO (use PTFE). Nylon (Polyamide): Low extractables, broad chemical compatibility including mixed aqueous-organic solvents, methanol, acetonitrile, and DMSO-water mixtures. Standard for HPLC mobile phase filtration and dissolution testing. Higher protein binding than PES or PVDF &mdash; avoid for biological samples where protein loss is a concern. MCE (Mixed Cellulose Ester): Low cost, aqueous applications only; moderate protein binding. Suitable for general buffer preparation, water sterilization, and non-critical sample preparation. Not compatible with organic solvents or strong acids. CA (Cellulose Acetate): Low protein binding, similar to PES. Suited for biological sample filtration and sterility testing applications. Contains surfactant treatment for wettability &mdash; check for extractable compatibility in downstream assays. PTFE (Polytetrafluoroethylene, hydrophilic): Maximum chemical resistance &mdash; compatible with pure DMSO, concentrated acids and bases, and aggressive organic solvents. The only membrane choice for 100% organic solvent filtration. For aqueous solutions, use hydrophilic PTFE (pre-wetted with methanol or IPA before aqueous filtration unless the hydrophilic version is selected). Sterile vs. Non-Sterile Syringe Filters Sterile syringe filters (individually gamma-irradiated or E-beam sterilized, individually packaged) are required for cell culture media sterilization, supplement preparation, and sample preparation for biological culture. Non-sterile bulk-packed filters (Globe Scientific non-sterile formats) are appropriate for HPLC mobile phase preparation, analytical sample clarification, and non-biological applications where sterility of the filtrate is not required. 
<h2 dir="ltr">Specifications Context</h2>
 Globe Scientific syringe filters are manufactured in a PP housing with a luer-lock inlet and outlet conforming to ISO 594 / ISO 80369 for Luer fittings. PES and PVDF sterile filters are individually gamma irradiated; each is separately wrapped and printed with an expiry date per Globe Scientific lot protocol. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 30 mm filter &asymp; 4.91 cm&sup2; (Globe Scientific welded format). Operating temperature: up to 60 &deg;C. Do not exceed the maximum operating pressure of 6 bar (87 psi). Globe Scientific is ISO 9001:2015 certified. Globe Scientific syringe filters in PES, PVDF, CA, MCE, and nylon membranes at 13 mm and 30 mm diameters are stocked by MBP. 
Request a quote today for premium syringe filters from <a href="https://mbpinc.net/contact-us">the MBP team</a> to&nbsp;find the right filtration solution for your laboratory applications.

SF-NYLN-2230-Group-Syringe Filters Brochure

Non-Sterile NYLN Syringe Filters

Diamond PureFlow syringe filters, Nylon Membrane, non-Sterile

Diamond PureFlow Non-Sterile Nylon Syringe Filters — hydrophilic nylon membrane with PP pre-filter. Available in 0.22 μm and 0.45 μm pore sizes; 13 mm and 30 mm diameters. PP housing, Female Luer Lock / Male Luer Slip. Ideal for HPLC and analytical sample preparation. SKUs: SF-NYLN-2213/2230 (0.22µm, 13mm/30mm), SF-NYLN-4513/4530 (0.45µm, 13mm/30mm).

Manufacture

Syringe Filter, Nylon Membrane, 0.22μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, Nylon Membrane, 0.45μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, Nylon Membrane, 0.22μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, Nylon Membrane, 0.45μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

SF-PTFE-2213-Group-Syringe Filters Brochure

Non-Sterile PTFE Syringe Filters

Diamond PureFlow syringe filters, PTFE Hydrophilic Membrane, non-Sterile

The Diamond PureFlow Non-Sterile Hydrophilic PTFE Syringe Filters by Globe Scientific provide the broadest chemical compatibility for HPLC sample prep, aggressive solvents, and analytical filtration. Available as SF-PTFE-2213 (0.22 µm, 13 mm), SF-PTFE-2230 (0.22 µm, 30 mm), SF-PTFE-4513 (0.45 µm, 13 mm), and SF-PTFE-4530 (0.45 µm, 30 mm). PP housing, bulk packed, 1,000/case.

Syringe Filter, PTFE Hydrophilic Membrane, 0.22μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophilic Membrane, 0.45μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophilic Membrane, 0.45μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophilic Membrane, 0.22μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

SF-PTFE-H-2213-Group-Syringe Filters Brochure

Non-Sterile PTFE-H Syringe Filters

Diamond PureFlow syringe filters, PTFE Hydrophobic Membrane, non-Sterile

Diamond PureFlow PTFE Hydrophobic Syringe Filters are non-sterile hydrophobic PTFE membrane syringe filters with a polypropylene microfiber pre-filter, designed for HPLC sample preparation, aggressive solvent filtration, and analytical instrument protection. Available in 0.22µm and 0.45µm pore sizes and 13mm and 25mm diameters, they deliver high flow rates with low sample loss. Clear housing markings identify membrane type and pore size at a glance. SKUs: SF-PTFE-H-2213 (0.22µm/13mm), SF-PTFE-H-2230 (0.22µm/30mm), SF-PTFE-H-4513 (0.45µm/13mm), SF-PTFE-H-4530 (0.45µm/30mm).

Syringe Filter, PTFE Hydrophobic Membrane, 0.22μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophobic Membrane, 0.22μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophobic Membrane, 0.45μm Porosity, 13mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Syringe Filter, PTFE Hydrophobic Membrane, 0.45μm Porosity, 30mm Diameter, PP Housing, Non-sterile, Bulk Packed, 1000/Cs

Vacuum Filtration

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
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<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Product Literature

PES Filter Specifications

Diamond PureFlow Vacuum Filter System, PES Membrane, Sterile

The Diamond PureFlow PES Vacuum Filter System Series by Globe Scientific is a Diamond PureFlow™ sterile vacuum filter system with low-protein-binding PES membrane, available in 150 mL, 250 mL, 500 mL, and 1,000 mL volumes with 0.10 µm, 0.22 µm, or 0.45 µm pore sizes. Each system includes a filter unit, graduated collection bottle, and sterile cap in USP Class VI materials. Available models: VF-FS-PES-0150-10, VF-FS-PES-0250-10, VF-FS-PES-0500-10, VF-FS-PES-1000-10, VF-FS-PES-0150-22, VF-FS-PES-0250-22, VF-FS-PES-0500-22, VF-FS-PES-1000-22, VF-FS-PES-0150-45, VF-FS-PES-0250-45, VF-FS-PES-0500-45, VF-FS-PES-1000-45.

Diamond PureFlow Vacuum Filter System, 150mL, PES Membrane,  0.10μm  Porosity, STERILE, Individually Wrapped, 100/Cs

Diamond PureFlow Vacuum Filter System, 250mL, PES Membrane,  0.10μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 500mL, PES Membrane,  0.10μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 1000mL, PES Membrane,  0.10μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 150mL, PES Membrane,  0.22μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 250mL, PES Membrane,  0.22μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 500mL, PES Membrane,  0.22μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 1000mL, PES Membrane,  0.22μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 150mL, PES Membrane,  0.45μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 250mL, PES Membrane,  0.45μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 500mL, PES Membrane,  0.45μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Diamond PureFlow Vacuum Filter System, 1000mL, PES Membrane,  0.45μm  Porosity, STERILE, Individually Wrapped, 12/Cs

Jerricans

Flyer

Jerricans Specifications

Diamond RealSeal™ Plastic Jerricans

The Diamond RealSeal&#8482; Plastic Jerrican by Globe Scientific is a rectangular HDPE jerrican with molded handle, angled spout, and leakproof PP RealSeal cap, rated to &#8722;100 &#176;C. Available in 5 L (7190005, CS/6), 10 L (7190010, CS/6), and 20 L (7190020, CS/4).

Jerrican with Handle, HDPE Bottle, 53mm PP White Screw Cap, 5 Litres (1.25 Gallons) , CS/6

Jerrican with Handle, HDPE Bottle, 53mm PP White Screw Cap, 10 Litres (2.5 Gallons), CS/6

Jerrican with Handle, HDPE Bottle, 53mm PP White Screw Cap, 20 Litres (5 Gallons), CS/4

Weighing Dishes & Boats

<h2 data-section-id="4vlupf" data-start="410" data-end="461">What Are Laboratory Weighing Dishes and Funnels?</h2>
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Laboratory weighing dishes and funnels are disposable antistatic consumables used in gravimetric sample preparation&mdash;where solid or liquid materials are weighed on analytical or top-loading balances before being transferred into reaction vessels, volumetric flasks, or tubes. Weighing dishes (also called weigh boats or pour boats) are flat-bottomed antistatic polystyrene containers used to hold samples on the balance pan during weighing. Weighing funnels are antistatic polypropylene tools with one flat side for stability on the balance and a conical stem designed to fit flask necks, allowing complete quantitative transfer of material with minimal loss. Globe Scientific offers antistatic PS weighing dishes in square, hexagonal, and pour-boat formats ranging from 10 mL to 350 mL, and antistatic PP weighing funnels in extra-small through extra-extra-large sizes. Both are single-use only because washing removes antistatic properties and affects weighing accuracy.
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<h2 data-path-to-node="15">What you will find:</h2>
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<a href="https://mbpinc.net/consumables/weighing-dishes-and-funnels/weighing-dishes" title="Weighing Dishes" style="color: #236fa1;">Weighing Dishes</a>: The Precision Interface &ndash; In the journey from balance to vessel, every milligram counts. Our dishes are the specialized bridges that ensure your "calculated mass" actually reaches the reaction.
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<a href="https://mbpinc.net/consumables/weighing-dishes-and-funnels/funnels" title="Funnels" style="color: #236fa1; text-decoration: underline;">Funnels</a>: High-Flow Transfer Logic &ndash; Stop the "glugging" and the spills. Our funnels are engineered to handle the physics of fluid and powder movement for a seamless bench workflow.
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<h2 data-section-id="1yz3s79" data-start="1436" data-end="1480">How to Choose Weighing Dishes and Funnels</h2>
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Weighing Dish Shape
Square weigh boats are used for routine laboratory weighing and are designed with sloping sides and recessed corners to minimize sample loss during transfer. Hexagonal weigh boats provide better stability on balance pans due to their molded edges and also offer improved grip during handling, making them suitable for workflows where stability matters. Pour-boat designs include a built-in spout that allows controlled and accurate dispensing of liquids or viscous samples into receiving vessels without spillage.
Weighing Dish Capacity
Small 10&ndash;20 mL dishes are used for micro-scale weighing of reagents typically under 2 g on analytical balances. Medium 50&ndash;100 mL dishes are used for routine laboratory weighing of 2&ndash;20 g samples. Large 200&ndash;350 mL dishes are used for bulk powders and higher-volume transfers on top-loading balances.
Weighing Funnel Size
Extra-small funnels are used for 5&ndash;20 mL vessels and microtubes, small funnels for 20&ndash;50 mL, medium funnels for 50&ndash;200 mL, large funnels for 200&ndash;500 mL, and extra-large funnels for 500&ndash;1000 mL flasks. The flat side ensures stable placement on the balance pan during weighing before transfer.
Color: White vs. Black
White dishes provide high contrast for dark or strongly colored samples, making them easier to visualize during transfer. Black dishes are used for light-colored or transparent samples where residue visibility is important, especially when confirming complete sample recovery.
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<h2 data-section-id="1jl751w" data-start="3653" data-end="3678">Specifications Context</h2>
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Globe Scientific antistatic polystyrene weighing dishes operate within &minus;10&deg;C to +80&deg;C and resist dilute acids, bases, and alcohols. The antistatic formulation is a specialized grade designed to reduce static charge and prevent sample loss during weighing and cannot be replaced with standard polystyrene labware. As of 2026, 20 mL and 100 mL antistatic weigh boats are widely used in pharmaceutical QC laboratories, research facilities, and academic analytical chemistry workflows where accuracy and contamination-free handling are required.
Keep sample handling clean and consistent&mdash;<a href="https://mbpinc.net/contact-us" title="MBP supports your workflow">MBP supports your workflow</a> &nbsp;with reliable weighing dishes and funnels designed for real lab conditions.

Weighing Dishes & Funnels

Weigh Dish Brochure

Diamond Shaped Weighing Boats

The Diamond Shaped Weighing Boats by Globe Scientific are antistatic white PS weighing boats with a tapered-both-ends diamond profile for directed powder transfer into narrow-necked vessels. Three volumes: 3614-5 (5 mL, CS/8,000), 3614-30 (30 mL, CS/4,000), 3614-100 (100 mL, CS/3,000).

Weight Boat, Diamond Shaped, Antistatic, PS, White, 5mL, CS/8000

Weight Boat, Diamond Shaped, Antistatic, PS, White, 30mL, CS/4000

Weight Boat, Diamond Shaped, Antistatic, PS, White, 100mL, CS/3000

<h2 dir="ltr">What Are Bottle Top Dispensers?</h2>
 A bottle top dispenser is a recirculating piston pump that screws onto a reagent supply bottle and dispenses a preset volume of liquid with each plunger stroke. The piston draws liquid up through an inlet valve from the bottle and delivers a fixed volume through a discharge tube on each downstroke; unused liquid is returned to the bottle via a recirculation valve, minimising waste. They replace repetitive pipetting from large reagent bottles, reducing operator exposure, evaporation of volatile solvents, and the risk of contaminating the bulk stock. Volume ranges span 0.2&ndash;100 mL, depending on cylinder size, covering reagent preparation, media dispensing, buffer aliquoting, and acid/base chemistry. Use a bottle top dispenser whenever you dispense the same volume repeatedly from a large bottle &mdash; it reduces operator time, contamination risk, and reagent waste versus manual pipetting.
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<h2 dir="ltr">What you will find:</h2>
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Adjustable volume bottle top dispensers
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Fixed volume liquid dispensers
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Chemical-resistant dispensers for harsh reagents
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Autoclavable bottle top dispensers
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Dispensers with recirculation valves
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High-precision piston-based dispensers
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Dispensers compatible with standard reagent bottles
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Easy-calibration bottle top dispensers
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<h2 dir="ltr"> How to Choose a Bottle Top Dispenser</h2>
 Volume Range: Match Cylinder Size to Application Dispensers are available in six nominal cylinder sizes: 1 mL, 2 mL, 5 mL, 10 mL, 25 mL, 50 mL, and 100 mL. Choose the cylinder size closest to your target dispensing volume; setting a dispenser to 20% of nominal volume introduces greater inaccuracy than setting it to 50&ndash;80% of nominal. The BRAND Dispensette S spans 1&ndash;100 mL across six sizes; Eppendorf Varispenser 2 covers 0.2&ndash;100 mL. Analog vs Digital Volume Setting Analog dispensers use a mechanical, scalloped, or graduated track for volume selection &mdash; simple, battery-free, and reliable for routine dispensing. Digital dispensers display the selected volume on an LCD screen, enabling precise sub-mL volume setting and easy documentation for GLP/GMP compliance contexts. Chemical Compatibility: Material Selection Standard dispensers use polypropylene (PP) valve bodies &mdash; suitable for aqueous buffers, saline, most bases, and many organic solvents. For aggressive acids (HCl, HNO₃, HF) and halogenated solvents, select dispensers with glass cylinders and PTFE or FEP wetted parts. Dispensette S Organic (BRAND) uses a PTFE-coated cylinder and FEP discharge tube for aggressive solvents and acids; Dispensette S Trace Analysis uses borosilicate glass for high-purity acid dispensing. GL 45 Thread Compatibility Most dispensers mount on GL 45-threaded bottles (the standard for Schott Duran, BRAND, and Nalgene reagent bottles). Thread adapters (GL 24&ndash;GL 45) are typically included for compatibility with smaller bottle sizes. Recirculation Valve A recirculation valve returns excess liquid to the supply bottle without re-aspiration, maintaining dispenser prime and reducing waste &mdash; standard on most modern dispensers and required when dispensing volatile reagents. 
<h2 dir="ltr"> Specifications Context</h2>
 Volume accuracy of a well-maintained bottle top dispenser is typically &plusmn;1% of nominal volume at 20&deg;C with distilled water (BRAND Dispensette S specification). ISO 8655 Part 5 defines performance standards for piston-operated burettes; bottle top dispensers that conform to this standard carry a performance certificate. GL 45 thread (DIN EN ISO 4161) is the global standard for reagent bottle neck finish. BRAND Dispensette S and Eppendorf Varispenser 2 remain the dominant bottle top dispenser formats in North American research labs.&nbsp; 
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Precision starts at the source. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Connect with the MBP team</a> to find the right dispenser for your lab and request a customized quote.

Bottle Top Dispensers

GBTD-00-Group-Diamond SureFlow Bottle Top Dispenser Brochure

User Manual

Diamond SureFlow Bottle Top Dispenser

The Diamond SureFlow Bottle Top Dispenser (GBTD) Series by Diamond&#174; is the SureFlow&#8482; bottle top dispenser in standard and recirculating models, covering 0.25&#8211;100 mL. Features a springless valve, removable piston for cleaning without recalibration, 360&#176; in-situ recalibration, dual graduations, volume lock, and 4 bottle thread adapters. Autoclavable; 2-year warranty. Select the volume range at order; request a quote or order at mbpinc.net or contact customerservice@mbpinc.net. Available models: GBTD-2.5, GBTD-05, GBTD-10, GBTD-25, GBTD-30, GBTD-50, GBTD-60, GBTD-100.

Bottle Top Dispenser, Diamond SureFlow, Standard, 0.25mL to 2.5mL, 0.05mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 0.5mL to 5.0mL, 0.1mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 1.0mL to 10.0mL, 0.2mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 2.5mL to 25.0mL, 0.5mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 2.5mL to 30.0mL, 0.5mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 5.0mL to 50.0mL, 1.0mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 5.0mL to 60.0mL, 1.0mL increments

Bottle Top Dispenser, Diamond SureFlow, Standard, 10.0mL to 100.0mL, 2.0mL increments

Diamond SureFlow Bottle Top Dispenser Accessories

The Diamond SureFlow Bottle Top Dispenser Accessories Series by Globe Scientific is the Diamond SureFlow Bottle Top Dispenser Accessories range — bottle adapters in 28mm, 38mm, 40mm, and 45mm thread sizes, compatible with Standard and Recirculating Diamond SureFlow dispensers. Also includes calibration tool (GBTD-CALTOOL), recirculation tube (GBTD-TUBE-RECIRC), and telescopic fill tube (GBTD-TUBE-TELES). Autoclavable. Order from MBP, USA. Available models: GBTD-ADP-28, GBTD-ADP-38, GBTD-ADP-40, GBTD-ADP-45, GBTD-CALTOOL, GBTD-TUBE-RECIRC, GBTD-TUBE-TELES.

Bottle Top Dispenser Adaptor, 28mm

Bottle Top Dispenser Adaptor, 38mm

Bottle Top Dispenser Adaptor, 40mm

Bottle Top Dispenser Adaptor, 45mm

Bottle Top Dispenser Calibration Tool

Bottle Top Dispenser Recirculation Tube

Bottle Top Dispenser Telescopic Fill Tube, 2.5mL to 60mL 

GBTD-R-00-Group-Diamond SureFlow Bottle Top Dispenser Brochure

Diamond SureFlow Bottle Top Dispenser, with Recirculation

The Diamond SureFlow™ Bottle Top Dispenser with Recirculation by Globe Scientific features a springless valve, ±0.5% accuracy, full autoclavability, and recirculation to eliminate priming waste. Available in eight volume ranges: GBTD-R-2.5 (0.25–2.5 mL), GBTD-R-05 (0.5–5 mL), GBTD-R-10 (1–10 mL), GBTD-R-25 (2.5–25 mL), GBTD-R-30 (2.5–30 mL), GBTD-R-50 (5–50 mL), GBTD-R-60 (5–60 mL), and GBTD-R-100 (10–100 mL).

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 0.25mL to 2.5mL, 0.05mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 0.5mL to 5.0mL, 0.1mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 1.0mL to 10.0mL, 0.2mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 2.5mL to 25.0mL, 0.5mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 2.5mL to 30.0mL, 0.5mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 5.0mL to 50.0mL, 1.0mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 5.0mL to 60.0mL, 1.0mL increments

Bottle Top Dispenser, Diamond SureFlow, with Recirculation, 10.0mL to 100.0mL, 2.0mL increments

Cell Culture Dishes

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
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Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

TC-DISH-Group-Diamond SureGro Cell & Tissue Culture Plastics Brochure

TC Treated Dish Specifications

Diamond SureGro Cell Culture Dish, Tissue Culture Treated

Diamond SureGro Cell Culture Dishes are tissue culture-treated cell culture dishes molded from virgin USP Class VI polystyrene, irradiation-sterilized to SAL 10⁻⁶, RNase-free, DNase-free, and non-pyrogenic. Available in five sizes from 35mm (8.5 cm²) to 150mm (143.0 cm²), with lid spacers for CO₂ exchange, stacking rings, and gripping rings on select sizes. Available sizes: TC-DISH-35 (35mm), TC-DISH-60 (60mm), TC-DISH-100 (100mm), TC-DISH-100XL (100mm XL), TC-DISH-150 (150mm).

Diamond SureGro Cell Culture Dish, Tissue Culture Treated, 35 x 10mm, Sterile, 10/Bag, 500/Case

Diamond SureGro Cell Culture Dish, Tissue Culture Treated, 60 x 15mm with Gripping Ring, Sterile, 10/Bag, 500/Case

Diamond SureGro Cell Culture Dish, Tissue Culture Treated, 100 x 20mm with Gripping Ring, Sterile, XL Growth Area, 10/Bag, 300/Case

Diamond SureGro Cell Culture Dish, Tissue Culture Treated, 100 x 15mm with Gripping Ring, Sterile, 10/Bag, 500/Case

Diamond SureGro Cell Culture Dish, Tissue Culture Treated, 150 x 20mm, Sterile, 5/Bag, 100/Case

Cell Culture Flasks

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
&nbsp;
<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
&nbsp;
Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

<h2 dir="ltr">What are Non-Treated Cell Culture Flasks?</h2>
 Non-treated cell culture flasks are sterile polystyrene T-flasks that retain the original hydrophobic, unmodified surface of the polystyrene substrate &mdash; no plasma, corona, or chemical surface treatment is applied. The hydrophobic surface minimizes cell attachment, making these flasks the correct vessel for cell types that grow in suspension or require minimal surface contact: suspension-adapted CHO, Jurkat, K562, HL-60, hematopoietic progenitors, hybridomas for monoclonal antibody production, insect cell lines (Sf9, Hi5), yeast, and bacteria. NEST Scientific non-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms, E-beam sterilized to SAL 10⁻⁶, and available in T25, T75, T175, and T225 sizes with vented (0.2 &micro;m PTFE membrane) or plug seal caps. 
Use non-treated flasks exclusively for cell types that do not require surface attachment; placing adherent mammalian cell lines in a non-treated flask causes cell death within 24&ndash;48 hours. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Suspension-Optimized Surface, like naturally hydrophobic polystyrene, ensures cells remain in suspension without surface interference.
</li>
<li dir="ltr" aria-level="1">
Scalable Formats precision-engineered in T25, T75, T175, and T225 sizes to fit various research scales.
</li>
<li dir="ltr" aria-level="1">
Aseptic Security with certified RNase-, DNase-, and Pyrogen-free to maintain the highest purity standards.
</li>
<li dir="ltr" aria-level="1">
Ergonomic Access with angled neck design facilitates seamless pipette access and reduces the risk of contamination during media changes.
</li>
</ul>
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">How to Choose Non-Treated Cell Culture Flasks</h2>
 Application: Suspension Mammalian Cells Suspension-adapted cell lines &mdash; CHO-S, HEK293-F, Expi293, and HEK293-EBNA variants used in transient transfection &mdash; require non-treated flasks for static suspension culture between spinner flask passages or when the scale is too small for orbital shaker flasks. Cells must be agitated (shaken, pipetted, or rocked) periodically to prevent settling when in static non-treated flasks with volumes &gt;5 mL. Application: Hematopoietic and Immune Cells Non-adherent hematopoietic cell lines (Jurkat T-cells, K562 erythroleukemia, HL-60 promyelocytes, Raji B-cells) and primary hematopoietic cells (peripheral blood mononuclear cells (PBMCs), bone marrow progenitors) must be cultured in non-treated vessels. TC-treated surfaces activate integrin signaling in hematopoietic cells, inducing undesired differentiation and stress responses. Application: Hybridoma Culture Hybridoma cells for monoclonal antibody (mAb) production are non-adherent B-cell fusions that grow as suspension cultures. Non-treated T75 and T225 flasks, combined with HT-supplemented DMEM/HAT medium, are the standard vessels for hybridoma selection and expansion. Scale-up from T225 non-treated flasks typically progresses to spinner or hollow-fiber bioreactors. Application: Insect Cells Sf9 (Spodoptera frugiperda) and Hi5 (Trichoplusia ni) insect cells used in baculovirus expression vector systems (BEVS) require non-treated polystyrene for monolayer culture. On TC-treated surfaces, Sf9 cells attach so strongly that they are difficult to recover. Non-treated flasks allow Sf9 to loosely adhere and detach easily with tapping, or to grow as a loosely attached monolayer that tolerates trypsin-free passaging. Application: Microbial Culture Non-treated polystyrene T-flasks with plug seal caps can serve as culture vessels for E. coli, Bacillus subtilis, yeast (S. cerevisiae, P. pastoris), and other microorganisms where the large surface area per volume is needed, and standard Erlenmeyer flask orbital shaking is unavailable. Ventilated caps support aerobic culture; confirm that the vented 0.2 &micro;m membrane is compatible with your aeration requirements. Cap Selection for Non-Treated Flasks Vented caps (0.2 &micro;m PTFE membrane) are standard for suspension cultures in a CO₂ incubator. For microbial aerobic culture without a CO₂ incubator, vented caps with 0.2 &micro;m pore still provide adequate aeration. Plug seal caps are used for anaerobic culture, transport, and short-term storage.
<h2> Specifications Context</h2>
 NEST Scientific non-treated cell culture flasks are manufactured from 100% virgin polystyrene with no surface modification. Contact angle of non-treated PS surface is approximately 75&ndash;90&deg; (hydrophobic), confirmed by water contact angle measurement per NEST lot QC data. E-beam sterilized in ISO 8 cleanrooms to SAL 10⁻⁶; DNase-free, RNase-free, and non-pyrogenic per lot certificate. Vented caps use a 0.2 &micro;m hydrophobic PTFE membrane. As of 2026, NEST Scientific non-treated flasks are stocked by MBP in all four sizes (T25, T75, T175, T225) with vented and plug seal options, in case quantities for PO-based institutional ordering. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Elevate your suspension culture workflows. <a href="https://mbpinc.net/contact-us">Contact Molecular Biology Products Inc.</a> for your Non-Treated Cell Culture Flasks today!

Non-Treated Cell Culture Flasks

Brochure

Non-Treated Flask Specifications

Diamond SureGro Cell Culture Flask, Non-treated

The Diamond SureGro Non-Treated Cell Culture Flask Series by Globe Scientific is a Diamond SureGro™ non-treated cell culture flask with white vented cap, sterile, RNase-free, DNase-free, and non-pyrogenic, designed for suspension cell culture in CO₂ incubators. Non-treated polystyrene surface prevents cell attachment for hybridoma, lymphocyte, and other non-adherent line propagation. For research use only. Available from MBP, Houston TX. Available models: TC-FLSK-25-V-NT, TC-FLSK-75-V-NT, TC-FLSK-182-V-NT, TC-FLSK-225-V-NT.

Diamond SureGro Cell Culture Flask, Non-treated, 50mL, Vent Cap, Sterile, 10/Bag, 200/Case

Diamond SureGro Cell Culture Flask, Non-treated, 250mL, Vent Cap, Sterile, 5/Bag, 100/Case

Diamond SureGro Cell Culture Flask, Non-treated, 600mL, Vent Cap, Sterile, 5/Bag, 40/Case

Diamond SureGro Cell Culture Flask, Non-treated, 850mL, Vent Cap, Sterile, 5/Bag, 25/Case

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
<li dir="ltr" aria-level="1">
Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
</li>
<li dir="ltr" aria-level="1">
Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
</li>
<li dir="ltr" aria-level="1">
Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
</li>
<li dir="ltr" aria-level="1">
Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
</li>
<li dir="ltr" aria-level="1">
Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

TC Treated Flask Specifications

Diamond SureGro Cell Culture Flask, Tissue Culture Treated

Diamond SureGro™ Cell Culture Flasks TC Treated (TC-FLSK-V) – tissue-culture-treated polystyrene flasks with enhanced SureGro™ surface for superior cell attachment and monolayer formation. Available in T-25, T-75, T-150, and T-175 sizes. Vented cap with hydrophobic membrane. Gamma-irradiated, sterile, non-cytotoxic, non-pyrogenic, DNase/RNase-free. Angled neck, optically clear base. Labcon. Available in 25, 75, 182, and 225 cm² with Vent Cap (-V) or Plug Cap (-P): TC-FLSK-25-V, TC-FLSK-25-P, TC-FLSK-75-V, TC-FLSK-75-P, TC-FLSK-182-V, TC-FLSK-182-P, TC-FLSK-225-V, TC-FLSK-225-P.

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 25cm2, Vent Cap, Sterile, 10/Bag, 200/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 75cm2, Vent Cap, Sterile, 5/Bag, 100/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 182cm2, Vent Cap, Sterile, 5/Bag, 40/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 225cm2, Vent Cap, Sterile, 5/Bag, 25/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 25cm2, Plug Cap, Sterile, 10/Bag, 200/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 75cm2, Plug Cap, Sterile, 5/Bag, 100/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 182cm2, Plug Cap, Sterile, 5/Bag, 40/Case

Diamond SureGro Cell Culture Flask, Tissue Culture Treated, 225cm2, Plug Cap, Sterile, 5/Bag, 25/Case

United States

Texas

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