PCR Reagents & Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

cDNA Synthesis Kits

Product Literature

1st cDNA synthesis kit

1st cDNA Synthesis Kit (FS01-00) by MBP contains all the components needed for first-strand cDNA synthesis. Available in 5 (FS01-00), 20 (FS01-01), 50 (FS01-02), and 100 (FS01-03) prep sizes. Ships on ice.

Manufacturer

1st cDNA synthesis kit, 5 Preps

1st cDNA synthesis kit, 20 Preps

1st cDNA synthesis kit, 50 Preps

1st cDNA synthesis kit, 100 Preps

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

Taq PCR Master Mixes

User Manual

2x Taq PCR Mix

2x Taq PCR Mix (TP01-00) by MBP is a ready-to-use 2× PCR master mix containing Taq at 5 u/µL, 10× Buffer, 20 mM MgSO4, and loading dye. Available in 100 µl (TP01-00), 5×1 mL (TP01-01), and 20×1 mL (TP01-02). Ships on ice.

2x Taq PCR Mix, 100ul

2x Taq PCR Mix, 5 x 1 mL

2x Taq PCR Mix, 20 x 1 mL

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

SYBR/Dye-Based

2x qPCR Mix SBGR

2x qPCR Mix SBGR (QP1311-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification of target sequences. Available in 100-reaction (QP1311-01) and 500-reaction (QP1311-02) sizes. Ships on ice.

2x qPCR Mix SBGR, 100 Reactions

2x qPCR Mix SBGR, 500 Reactions

QP1313 Protocol

2x qPCR Mix, SBGR,  Rox

2x qPCR Mix SBGR Rox (QP1313-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification. Standard Rox formulation for instruments requiring a ROX reference. Available in 100-reaction (QP1313-01) and 500-reaction (QP1313-02) sizes. Ships on ice.

2x qPCR Mix, SBGR,  Rox, 100 Reactions

2x qPCR Mix, SBGR,  Rox, 500 Reactions

QP1312 Protocol

2x qPCR Mix, SBGR, Low Rox

2x qPCR Mix SBGR Low Rox (QP1312-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification. Low Rox formulation for instruments requiring a low ROX reference. Available in 100-reaction (QP1312-01) and 500-reaction (QP1312-02) sizes. Ships on ice.

2x qPCR Mix, SBGR, Low Rox, 100 Reactions

2x qPCR Mix, SBGR, Low Rox, 500 Reactions

<h2 dir="ltr">What Is a Mycoplasma PCR Detection Kit?</h2>
 A Mycoplasma PCR detection kit provides primers, master mix, ultrapure water, and a positive control for rapid PCR-based screening of cell culture samples for Mycoplasma contamination. Because Mycoplasma species lack a cell wall and do not produce visible turbidity or media discoloration, contamination frequently goes undetected without periodic molecular testing, making these kits a standard component of cell culture quality assurance programmes. Run a Mycoplasma PCR detection kit periodically on actively maintained cell lines and before banking, sharing, or publishing results from a cell line as routine quality assurance against a contaminant that produces no visible signs. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Detection &amp; Elimination Kits: All-encompassing systems such as Mycoplasma Pro crafted to accurately identify contamination while also enabling complete eradication of infected cultures.
</li>
<li dir="ltr" aria-level="1">
PCR Detection Kits: Standardized, validated reagents that provide a quick and economical solution for regular laboratory testing and quality assurance.
</li>
<li dir="ltr" aria-level="1">
Mycoplasma gDNA Kits: Tailored genomic DNA extraction solutions specifically designed for isolating low-abundance mycoplasma templates from intricate biological specimens.
</li>
</ul>
<h2 dir="ltr"> How to Choose a Mycoplasma PCR Detection Kit</h2>
 Species/Strain Coverage Kits vary in the breadth of Mycoplasma genera and species detected -- some formats detect over 200 strains, while enhanced ("Pro"-type) formats may extend coverage to 300+ strains, targeting conserved regions shared across common contaminant species such as M. arginini, M. hyorhinis, M. orale, and others. Sample Preparation Requirements Many Mycoplasma PCR detection kits allow direct addition of cell culture media to the PCR reaction without a separate DNA extraction step, simplifying the workflow and reducing time-to-result -- confirm whether your chosen kit requires extraction or supports direct sample addition. Internal Control Availability Enhanced kit formats may include an internal amplification control, providing additional confidence that a negative result reflects true absence of contamination rather than a failed or inhibited PCR reaction -- particularly valuable for samples with potential PCR inhibitors. Detection Sensitivity Standard kits can detect contamination at typical levels found in actively growing contaminated cultures; ultra-sensitive qPCR-based Mycoplasma detection formats (a related but distinct product type) can detect as few as 10 genome copies directly from media for earlier-stage contamination detection. Result Interpretation A positive result is typically indicated by a PCR product in a defined size range (commonly in the 260-550 bp range depending on the specific primer design), distinguishable from the positive control band; a clean no-template control validates assay specificity for each run. 
<h2 dir="ltr"> Specifications Context</h2>
 Mycoplasma PCR detection kits use primers highly specific to conserved rDNA regions across Mycoplasma genomes, enabling a single primer mix to detect dozens to hundreds of species and strains -- including commonly encountered laboratory contaminants - in one reaction. The streamlined workflow (combine sample with master mix and primer mix, run PCR, analyse by gel electrophoresis) typically delivers results within 1-2 hours, in contrast to culture-based Mycoplasma testing methods that can take days to weeks. A positive result appears as a distinct PCR product within the kit's expected size range, while the included positive control confirms the reaction performed correctly even when test samples are negative. Routine screening intervals vary by institutional policy, but testing before banking a new cell line, after receiving cells from an external source, and periodically during long-term culture are common checkpoints. Mycoplasma PCR detection kits are typically stored at &minus;20 &deg;C; positive controls are included with the kit. 
Safeguard the integrity of your culture&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">reach out to the MBP team</a> now for a quote on our expert Mycoplasma detection services.

Mycoplasma PCR Detection Kit

Mycoplasma gDNA Kit

Mycoplasma gDNA Kit (GD3211-00) by MBP is designed for purification of genomic DNA from Mycoplasma samples. Available in 4 (GD3211-00), 50 (GD3211-01), and 250 (GD3211-02) prep sizes.

Mycoplasma gDNA Kit, 4 Preps

Mycoplasma gDNA Kit, 50 Preps

Mycoplasma gDNA Kit, 250 Preps

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

One-Step RT-PCR Kits

RT0201.IFU

One-Step RT-PCR Kit

One-Step RT-PCR Kit (RT0201-00) by MBP uses MMLV Reverse Transcriptase and Hot start Taq DNA polymerase for single-tube RT-PCR from total RNA or mRNA. Input RNA: 0.01 pg–2 µg; detects targets up to 4.0 kb; 100 reactions per kit. Available in 4 (RT0201-00), 50 (RT0201-01), and 250 (RT0201-02) prep sizes. Ships on ice.

One-Step RT-PCR Kit, 4 Preps

One-Step RT-PCR Kit, 50 Preps

One-Step RT-PCR Kit, 250 Preps

<h2 dir="ltr">What Is Taq DNA Polymerase?</h2>
 Taq DNA polymerase, isolated from the thermophilic bacterium Thermus aquaticus, was the enzyme that made modern PCR possible. Its thermostability &mdash; retaining activity after repeated denaturation cycles at 94-95&deg;C &mdash; eliminated the need to add fresh enzyme after each cycle, a limitation of earlier PCR approaches using non-thermostable polymerases.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
High-Purity Enzymes: Versatile standalone polymerases, including Azura&trade; and Accuris series, available in flexible formats from 500 to 6000 units to suit both small-scale projects and high-volume industrial applications.
</li>
<li dir="ltr" aria-level="1">
Optimized Performance: Specialized formulations designed for superior processivity and flexible reaction tuning, allowing for high-yield synthesis across diverse and challenging DNA templates.
</li>
</ul>
<h2 dir="ltr"> How to Choose Taq DNA Polymerase</h2>
 Standard vs. Hot-Start Standard Taq is active immediately at room temperature, suitable for most routine genotyping and screening applications. Hot-start Taq formulations remain reversibly inactive until a high-temperature activation step, improving specificity for multiplex or low-abundance template reactions. Buffer System Compatibility Optimised 10x PCR buffers are formulated to complement the enzyme's activity profile &mdash; using mismatched buffer systems from other suppliers can affect yield and specificity, so matched buffer-enzyme pairs are recommended. MgCl2 Titration Needs MgCl2 concentration is one of the most influential variables in PCR specificity and yield. Separate MgCl2 solutions allow titration for templates or primer sets requiring optimisation beyond standard buffer concentrations. Concentration and Unit Format Enzyme is typically supplied in units (U) reflecting incorporation activity per unit time &mdash; confirm the unit concentration matches your typical reaction scale to avoid excessive dilution or waste. Application Scope Genotyping, colony screening after transformation, diagnostic band confirmation, and general routine amplification are the primary applications for standard and hot-start Taq DNA polymerase. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Taq DNA polymerase&rsquo;s thermostability &mdash; the ability to withstand repeated 94&ndash;95 &deg;C denaturation cycles without loss of activity &mdash; enabled practical PCR workflows based on a single thermostable enzyme. The enzyme lacks 3&prime;&rarr;5&prime; proofreading exonuclease activity, resulting in a higher error rate than high-fidelity polymerases (e.g., Phusion, Q5, KOD, Pfu-based systems), making it suitable for genotyping and screening but less appropriate for cloning- or sequencing-grade amplification.
Standard storage is at &minus;20 &deg;C, and many formulations are generally tolerant to multiple freeze&ndash;thaw cycles depending on buffer composition; lot-specific CoAs are available on request. In laboratories using both standard and hot-start Taq formulations, clear labelling and separation of storage conditions help prevent cross-use, particularly in assays where hot-start specificity is critical. A side-by-side validation on a known template when adopting a new Taq formulation can help establish expected amplification efficiency and specificity before routine use. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
Refine your amplification results&mdash;<a href="https://mbpinc.net/contact-us" title="contact the MBP team" style="color: #236fa1;">contact the MBP team</a> today for a quote on our professional Taq Polymerase solutions.

Taq DNA Polymerase

Taq DNA Polymerase (E121-00) by MBP is supplied at 5 u/µL with 10&times; Buffer and MgSO4. Available in 50 U (E121-00), 500 U (E121-01), 1,000 U (E121-02), and 5,000 U (E121-03) pack sizes. Ships on ice.

Taq DNA Polymerase , 50 U

Taq DNA Polymerase , 500 U

Taq DNA Polymerase , 1000 U

Taq DNA Polymerase ,5000 U

<h2 dir="ltr"> What is a dNTP Mix?</h2>
 A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing.&nbsp; Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand&trade; Ultra-Pure dNTP Mix (Azura)</li>
<li>Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats</li>
<li>Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility</li>
<li>Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions</li>
<li>Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications</li>
</ul>
&nbsp;
<h2>How to Choose a dNTP Mix</h2>
 Concentration Format Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions. Equimolar Accuracy Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation. Throughput Considerations For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility. Compatibility with Master Mixes Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix. Application Range A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable. 
<h2 dir="ltr"> Specifications Context</h2>
 To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 &micro;L of the premix is added per 50 &micro;L reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at &minus;20 &deg;C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.

dNTPs Mix

dNTP Mix, 10mM each

dNTP Mix (NT01-00) by MBP is a mixture of dATP, dCTP, dTTP and dGTP, each at 10 mM. Available in 50 µl (NT01-00), 500 µl (NT01-01), 2 mL (NT01-02), and 5 mL (NT01-03). Ships on ice.