Name: Taq DNA Polymerase
SKU: E121-00
Availability: InStock

<h2 dir="ltr">What Is Taq DNA Polymerase?</h2>
 Taq DNA polymerase, isolated from the thermophilic bacterium Thermus aquaticus, was the enzyme that made modern PCR possible. Its thermostability &mdash; retaining activity after repeated denaturation cycles at 94-95&deg;C &mdash; eliminated the need to add fresh enzyme after each cycle, a limitation of earlier PCR approaches using non-thermostable polymerases.
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<h2 dir="ltr">What you will find:</h2>
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High-Purity Enzymes: Versatile standalone polymerases, including Azura&trade; and Accuris series, available in flexible formats from 500 to 6000 units to suit both small-scale projects and high-volume industrial applications.
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Optimized Performance: Specialized formulations designed for superior processivity and flexible reaction tuning, allowing for high-yield synthesis across diverse and challenging DNA templates.
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<h2 dir="ltr"> How to Choose Taq DNA Polymerase</h2>
 Standard vs. Hot-Start Standard Taq is active immediately at room temperature, suitable for most routine genotyping and screening applications. Hot-start Taq formulations remain reversibly inactive until a high-temperature activation step, improving specificity for multiplex or low-abundance template reactions. Buffer System Compatibility Optimised 10x PCR buffers are formulated to complement the enzyme's activity profile &mdash; using mismatched buffer systems from other suppliers can affect yield and specificity, so matched buffer-enzyme pairs are recommended. MgCl2 Titration Needs MgCl2 concentration is one of the most influential variables in PCR specificity and yield. Separate MgCl2 solutions allow titration for templates or primer sets requiring optimisation beyond standard buffer concentrations. Concentration and Unit Format Enzyme is typically supplied in units (U) reflecting incorporation activity per unit time &mdash; confirm the unit concentration matches your typical reaction scale to avoid excessive dilution or waste. Application Scope Genotyping, colony screening after transformation, diagnostic band confirmation, and general routine amplification are the primary applications for standard and hot-start Taq DNA polymerase. 
<h2 dir="ltr"> Specifications Context</h2>
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Taq DNA polymerase&rsquo;s thermostability &mdash; the ability to withstand repeated 94&ndash;95 &deg;C denaturation cycles without loss of activity &mdash; enabled practical PCR workflows based on a single thermostable enzyme. The enzyme lacks 3&prime;&rarr;5&prime; proofreading exonuclease activity, resulting in a higher error rate than high-fidelity polymerases (e.g., Phusion, Q5, KOD, Pfu-based systems), making it suitable for genotyping and screening but less appropriate for cloning- or sequencing-grade amplification.
Standard storage is at &minus;20 &deg;C, and many formulations are generally tolerant to multiple freeze&ndash;thaw cycles depending on buffer composition; lot-specific CoAs are available on request. In laboratories using both standard and hot-start Taq formulations, clear labelling and separation of storage conditions help prevent cross-use, particularly in assays where hot-start specificity is critical. A side-by-side validation on a known template when adopting a new Taq formulation can help establish expected amplification efficiency and specificity before routine use. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
Refine your amplification results&mdash;<a href="https://mbpinc.net/contact-us" title="contact the MBP team" style="color: #236fa1;">contact the MBP team</a> today for a quote on our professional Taq Polymerase solutions.

Taq DNA Polymerase

Taq DNA Polymerase (E121-00) by MBP is supplied at 5 u/µL with 10&times; Buffer and MgSO4. Available in 50 U (E121-00), 500 U (E121-01), 1,000 U (E121-02), and 5,000 U (E121-03) pack sizes. Ships on ice.