Cell Culture

<h2 class="p1"> What are Cell Strainers?</h2>
 Cell strainers are sterile, single-use nylon mesh filter caps that sit in the opening of a 50 mL conical centrifuge tube, used to disaggregate tissue homogenates, tumor biopsies, lymph nodes, spleens, and bone marrow aspirates into uniform single-cell suspensions for downstream applications including flow cytometry, primary cell culture, FACS sorting, and immunophenotyping. The strainer is placed filter-side down in the collection tube, the tissue suspension is added to the strainer basket and pushed through the nylon mesh using the plunger of a syringe, the wet end of a syringe barrel, or the back of a sterile 1 mL syringe. Choose 40 &micro;m for fine single-cell suspension preparation from soft tissues; 70 &micro;m for standard tissue disaggregation (the most commonly used pore size); 100 &micro;m for pre-filtration of coarse tissue homogenates and large spheroids.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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40&mu;m Cell Strainers for fine-scale molecular filtration
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70&mu;m Cell Strainers for routine primary cell isolation
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100&mu;m Cell Strainers for large cluster separation
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Sterile Nylon Mesh Strainers for precise pore consistency
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Color-Coded Strainers for easy micron-size identification
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Universal Fit Strainers for standard 50mL centrifuge tubes
</li>
</ul>
<h2 class="p1"> How to Choose Cell Strainers</h2>
 Pore Size by Application 40 &micro;m strainers (blue) produce the finest filtrate, retaining cell aggregates and larger tissue debris while passing single cells and small cell clusters. They are the standard for preparing highly uniform single-cell suspensions from soft tissues (spleen, thymus, lymph nodes) for flow cytometry, where doublets must be minimized. 40 &micro;m strainers have lower flow rates and clog more readily with dense or fibrous tissues. 70 &micro;m strainers (white) are the most commonly used pore size in cell biology &mdash; they balance efficient single-cell passage with adequate retention of fibrous connective tissue and undigested tissue chunks. Standard for tumor dissociation, liver perfusion single-cell prep, and any tissue where a moderate doublet rate is acceptable. 100 &micro;m strainers (yellow) pass cell clusters and small organoids while retaining large undissociated tissue pieces; used as a first-pass pre-filtration step before 70 &micro;m or 40 &micro;m filtration of tough fibrous tissues, and for filtering 3D tumor spheroids grown in 96-well U-bottom plates before reseeding. Color-Coding for Laboratory Workflow NEST Scientific cell strainers follow a universal color-coding convention: 40 &micro;m = blue, 70 &micro;m = white, 100 &micro;m = yellow. This matches the color convention used by BD Biosciences Falcon cell strainers, allowing unambiguous pore-size identification during multi-step tissue disaggregation protocols without reading the label at every step. 50 mL Tube Compatibility NEST Scientific cell strainers are designed with a flange diameter that fits securely into the opening of 50 mL conical centrifuge tubes from most major brands, including BD Falcon, TPP, and MidSci, per NEST Scientific product documentation. A molded tab on the upper ring facilitates sterile handling during tube loading without touching the mesh surface. Tissue Types and Recommended Pore Size For spleen, thymus, and lymph nodes: 40 &micro;m or 70 &micro;m. For bone marrow: 70 &micro;m. For tumor biopsy (mechanically or enzymatically dissociated): 40 &micro;m (after enzyme digestion) or 70 &micro;m (mechanical disaggregation only). For liver (perfusion-dissociated hepatocytes): 70 &micro;m or 100 &micro;m. For 3D spheroid disaggregation: 40 &micro;m or 70 &micro;m, depending on the desired cluster size in the filtrate. For flow cytometry sorting (minimize doublets): 40 &micro;m immediately before loading the sorter. 
<h2 class="p1"> Compare NEST Scientific Cell Strainer SKUs </h2>
<ul>
<li data-section-id="16rq5ic" data-start="0" data-end="126">40 &micro;m (Blue): NEST Catalog 258369; 50/case; suitable for fine single-cell suspension and flow cytometry pre-filtration.</li>
<li data-section-id="10iabbd" data-start="128" data-end="251">70 &micro;m (White): NEST Catalog 258368; 50/case; suitable for standard tissue disaggregation and universal applications.</li>
<li data-section-id="toszed" data-start="253" data-end="390" data-is-last-node="">100 &micro;m (Yellow): NEST Catalog 258367; 50/case; suitable for pre-filtration, coarse tissue processing, and spheroid size selection.</li>
</ul>
<h2 class="p1"> Specifications Context</h2>
 NEST Scientific cell strainers are manufactured from nylon mesh bonded to a polypropylene (PP) frame with a tab-molded upper ring for sterile handling. Pore sizes are 40 &micro;m, 70 &micro;m, and 100 &micro;m; mesh pore spacing is uniform per manufacturing lot. Compatible with 50 mL conical centrifuge tubes from BD Falcon, TPP, and MidSci per NEST Scientific USA documentation. Each strainer is sterile (gamma-irradiated), individually wrapped, non-pyrogenic, and DNase/RNase-free per lot certificate. Non-cytotoxic nylon mesh. All three pore sizes (40, 70, and 100 &micro;m) are stocked by MBP in cases of 50. 
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Cell Strainers

Biologix Cell Strainers

Biologix cell strainers, PP frame/nylon mesh, radiation sterile SAL 10⁻⁶, non-pyrogenic, individually wrapped: 40µm blue (93040), 70µm natural (93070), 100µm yellow (93100). Fits 50mL centrifuge tubes.

Cell Strainer, PP/Nylon, Blue, pore size 40μm, Sterile to SAL10-6. Individually wrapped.

Cell Strainer, PP/Nylon, Natural, pore size 70μm, Sterile to SAL10-6. Individually wrapped.

Cell Strainer, PP/Nylon, Yellow, pore size 100μm, Sterile to SAL10-6. Individually wrapped.

Biologix® Cell Strainers, 40/70/100μm, Individual Package

Biologix Cell Strainers, Support Your Science Experiments!

Biologix® Cell Strainers, Individual Package, Case of 100

Biologix Sterile Nylon Mesh Cell Strainers — 15-1040 (40 µm, purple), 15-1070 (70 µm, orange), 15-1100 (100 µm, green); PP frame with molded tab; compatible with 50 ml centrifuge tubes; individually wrapped; 100/case. For single-cell suspension preparation and tissue dissociation filtration.

Brand

Model

Material

Size

Sterile

BIOLOGIX 40µM STERILE NYLON MESH CELL STRAINER WITH PURPLE POLYPROPYLENE FRAME FEATURING MOLDED TAB FOR EASY HANDLING. (COMPATIBLE WITH BIOLOGIX 50ML CENTRIFUGE TUBES.) STRAINERS COME INDIVIDUALLY WRAPPED.  100/CASE

BIOLOGIX 70µM STERILE NYLON MESH CELL STRAINER WITH ORANGE POLYPROPYLENE FRAME FEATURING MOLDED TAB FOR EASY HANDLING. (COMPATIBLE WITH BIOLOGIX 50ML CENTRIFUGE TUBES.) STRAINERS COME INDIVIDUALLY WRAPPED.  100/CASE

BIOLOGIX 100µM STERILE NYLON MESH CELL STRAINER WITH GREEN POLYPROPYLENE FRAME FEATURING MOLDED TAB FOR EASY HANDLING. (COMPATIBLE WITH BIOLOGIX 50ML CENTRIFUGE TUBES.) STRAINERS COME INDIVIDUALLY WRAPPED.  100/CASE

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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50mL Vacuum Filtration Units for small-scale sterile sampling
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150mL Vacuum Filtration Systems for rapid reagent preparation
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250mL Filtration Units for routine buffer sterilization
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500mL Vacuum Filtration Cups for mid-scale media processing
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1000mL Large Volume Filtration for industrial-scale batching
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Sterile Bottle-Top Filters for secure aseptic transfer
</li>
</ul>
<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Vacuum Filtration

Vacuum filtration unit-funnel, membrane: PES, pore size: 0.22um, diameter: 90mm, funnel volume: 500ml.

Vacuum filtration unit-funnel, membrane: CA, pore size: 0.22um, diameter: 90mm, funnel volume: 500ml, receiver volume: 500ml.

Biologix® Vacuum Filtration Unit-Funnel

Biologix Sterile Vacuum Filtration Unit-Funnel 500 ml 0.22 µm 90 mm — 09-PES2250F (PES membrane, low protein binding) and 09-CA2250F (CA membrane); funnel-only replacement units; individually packed. For sterile filtration of cell culture media and biological buffers.

BIOLOGIX STERILE VACUUM FILTRATION UNIT-FUNNEL, MEMBRANE: PES,PORE SIZE: 0.22ΜM,DIAMETER:90MM, FUNNEL VOLUME: 500ML.   INDIVIDUALLY PACKED

BIOLOGIX STERILE VACUUM FILTRATION UNIT-FUNNEL, MEMBRANE: CA,PORE SIZE: 0.22ΜM,DIAMETER:90MM, FUNNEL VOLUME: 500ML.INDIVIDUALLY PACKED

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
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Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

Cell Culture Dishes

Cell Culture Dish, PS, TC Treated

PS TC-treated cell culture dishes — 35 mm (20035), 60 mm (20060), 90 mm (20100), 150 mm (03-1955). Sterile to SAL 10⁻⁶. External grip on 35 and 60 mm sizes. For adherent mammalian cell culture in all standard dish formats.

Cell Culture Dish,35x10mm, PS,TC treated, sterile to SAL 10-6, external grip, 10/bag, 50bags/case

Cell Culture Dish,60x10mm PS,TC treated, sterile to SAL 10-6, external grip, 10/bag, 50bags/case

Cell Culture Dish,90x20mm, PS,TC treated, sterile to SAL 10-6,  10/bag, 20bags/case

Cell Culture Dish,150x25mm, PS,TC treated, sterile to SAL 10-6,  10/bag, 12bags/case

Multi Inser Dishes

Multi Insert Dish

Multi Insert Dish — 60×20 mm, 1.77 cm², for intercellular co-culture and biosystem mimicking research. Standard (911605), with stirrer (911607), surface treated (911615), and low binding (911625). Sterile, 18/case.

Multi Insert Dish, 60X20mm, Growth Area 1.77㎠, 15ml, external Grip, Sterile, 18/Case

Multi Insert Dish with Stirrer, 60X20mm, Growth Area 1.77㎠, 15ml, Groove bottom, external Grip, Sterile, 18/Case

Multi Insert Dish Surface Treated, 60X20mm, Growth Area 1.77㎠, Sterile, 18/Case

Multi Insert Dish Low Binding, 60X20mm, Growth Area 1.77㎠, Sterile, 18/Case

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
&nbsp;
<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

Cell Culture Flasks

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
<li dir="ltr" aria-level="1">
Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
</li>
<li dir="ltr" aria-level="1">
Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
</li>
<li dir="ltr" aria-level="1">
Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
</li>
<li dir="ltr" aria-level="1">
Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
</li>
<li dir="ltr" aria-level="1">
Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

SPL Cell Culture Flask, with Filter or Plug Cap

SPL PS TC-treated cell culture flasks — 25, 75, and 175 cm² in filter cap (vented) and plug cap (sealed) configurations. Sterile to SAL 10⁻⁶, angled wide-neck design, excellent stackability. 5 flasks/bag.

Tissue Culture Flask with Plugged Cap, PS, 25cm2, TC treated, Sterile to SAL10-6, 5/bag, 40bags/case

Tissue Culture Flask with Plugged Cap, PS, 75cm2, TC treated, Sterile to SAL10-6, 5/bag, 20bags/case

Tissue Culture Flask with Plugged Cap, PS, 175cm2, TC treated, Sterile to SAL10-6, 5/bag, 8bags/case

Cell Culture Flask with Filter Cap,PS, 25cm2, TC treated, Sterile to SAL10-6. 5 Flasks/Bag, 40 Bags/Case

Cell Culture Flask with Filter Cap,PS, 75cm2, TC treated, Sterile to SAL10-6. 5 Flasks/Bag, 20 Bags/Case

Cell Culture Flask with Filter Cap,PS, 175cm2, TC treated, Sterile to SAL10-6.  5 Flasks/Bag, 8 Bags/Case

<h2 dir="ltr">What are Cell Culture Inserts?</h2>
 Cell culture inserts (also called Transwell inserts or permeable supports) are sterile, single-use polystyrene well inserts with a permeable membrane floor that suspend cells above the bottom of a standard multi-well plate, creating separate apical (upper, inside insert) and basolateral (lower, outside insert in the well below) compartments. The membrane allows diffusion of media, nutrients, metabolites, and signaling molecules between the two compartments while physically separating the two cell populations.&nbsp;Choose PET membranes for imaging and clear-bottom visualization; choose PC membranes for superior cell attachment; choose pore size based on whether cells need to migrate through the membrane (&ge;3 &micro;m) or be retained on it (0.4 &micro;m and 1 &micro;m).
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" role="presentation">6 Inserts / 6-Well Plate for Tissue Engineering - Ideal for skin models and complex cell differentiation where a larger surface area is required.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 12-Well Plate for Transport Assays - Balanced format for nutrient diffusion studies and metabolic monitoring.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 24-Well Plate for&nbsp;High Throughput Screening - Designed for migration and chemotaxis assays with efficient reagent use.</li>
<li style="color: #000000;">SPLInsert&trade; Hanging formats with transparent, sterile PC or PET membranes.</li>
<li style="color: #000000;">SPLInsert&trade; Standing format for cell culture insert applications.</li>
<li style="color: #000000;">100 mm cell culture insert dishes with 3.0 &mu;m PC membranes, translucent construction, tissue-culture treatment, and sterile packaging.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose Cell Culture Inserts</h2>
 PET vs. PC Membrane PET (polyethylene terephthalate) membranes are optically transparent, allowing direct visualization of cells growing on the membrane by standard phase-contrast and fluorescence microscopy without removing the insert. PET is the first choice when imaging cells on the membrane is part of the experimental readout (tight-junction immunofluorescence, GFP localization, wound-healing on the membrane). PC (polycarbonate) membranes provide superior cell attachment due to their higher surface energy &mdash; often used for primary epithelial, endothelial, and intestinal cells that are difficult to culture on PET. PC membranes are opaque, blocking transmitted light microscopy through the membrane. Pore Size by Application 0.4 &micro;m pore inserts prevent cell migration and cell-cell contact between apical and basolateral compartments while allowing free diffusion of molecules &lt;0.4 &micro;m (nutrients, cytokines, small molecules, metabolites). Standard for epithelial barrier function assays (TEER measurement, permeability assays), secretion studies (measuring cytokine secretion from the basolateral side), and any co-culture where cell-cell contact must be prevented. 1 &micro;m pore inserts similarly prevent cell migration but allow slightly larger molecule diffusion &mdash; used for exosome transport studies. 3 &micro;m pore inserts allow poorly migratory cells (some endothelial, fibroblast lines) to migrate through. 8 &micro;m pore inserts support tumor cell migration, invasion, chemotaxis, and hematotaxis assays &mdash; the standard pore size for Boyden chamber&ndash;style migration and invasion assays. Plate Format and Insert Configuration NEST Scientific inserts are pre-packed in multi-well plates: 6-well plate format (6 inserts per plate), 12-well plate format (12 inserts per plate), 24-well plate format (12 inserts per plate). Inserts are centered in wells by their flange geometry, preventing the membrane from touching the well sides and creating a wicking path. The apical-basolateral gap (distance from membrane floor to well bottom) is designed to allow media access to the basolateral face and pipette access to the lower chamber for sampling. TC-Treated vs. Non-Treated Membrane TC-treated membrane inserts promote adherent mammalian cell monolayer formation on the insert membrane. Non-treated membrane inserts (some PC formats) are used for migration and invasion assays where cells in the apical chamber must actively migrate through the membrane pore to reach the basolateral side &mdash; a non-treated surface reduces passive cell adhesion that would produce false-positive migration signals. Transferability NEST Scientific cell culture inserts can be transferred from one plate to a fresh plate for media changes, cumulative secretion studies, and time-course experiments &mdash; a key advantage over the non-insert well format. Transfer the insert using sterile forceps or the insert flange. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture inserts are manufactured from polystyrene with PET or PC membranes in TC-treated (vacuum plasma, contact angle &lt;10&deg;) or non-treated formats. Inserts are packed in individually sealed, peelable Tyvek film and blister packaging for sterility assurance &mdash; one plate per pack, sterile. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free and non-pyrogenic per lot. The insert flange geometry is designed to center the insert in the well, preventing membrane-wall contact and wicking. NEST Scientific cell culture inserts in PET and PC membranes across the four pore sizes (0.4, 1, 3, 8 &micro;m) and three plate formats (6-, 12-, 24-well) are available from MBP. 
&nbsp;
Bridge the gap between routine culture and physiological discovery. Optimize your laboratory procurement with&nbsp;Molecular Biology Products Inc. <a href="https://mbpinc.net/contact-us">Connect with our specialists</a> for a customized quote today.

Cell Culture Inserts

SPLInsert Hangings

SPLInsert™ Hanging, PC Membrane, Transparent, Sterile

SPLInsert™ Hanging Inserts with polycarbonate (PC) membrane by SPL Life Sciences are sterile, rim-supported transwell inserts in 6-well, 12-well, and 24-well formats at 3 µm or 8 µm pore sizes. Growth areas 0.33–4.52 cm²; membrane thickness 10–23 µm. Six SKUs (35106–35224). For cell migration, co-culture, and barrier function assays in research and biopharma labs.

SPLInsert™ Hanging, 6 Inserts/6 well plate, PC, 3μm, Growth Area 4.52㎠, 1.5~2.5ml, Translucent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PC, 8μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PC, 3.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PC, 8.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PC, 3μm, Growth Area 0.33㎠, 0.2~0.35ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PC, 8μm, Growth Area 0.33㎠, 0.2~0.35ml,  Translucent, Sterile, 48/Case

SPLInsert™ Hanging, PET Membrane, Transparent, Sterile

SPLInsert™ Hanging PET Membrane Inserts (37006 series) by SPL: transparent PS frame, PET membrane, low protein binding, sterile. Available for 6-well (4.52 cm²), 12-well (0.90 cm²), and 24-well (0.33 cm²) plates in 0.4, 3, and 8 µm pore sizes. 24–48 inserts/case.

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 0.4μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 3μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 8μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 0.4μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 3.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 8.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 0.4μm, Growth Area 0.33㎠, 0.2~0.35m, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 3μm, Growth Area 0.33㎠, 0.2~0.35ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 8μm, Growth Area 0.33㎠, 0.2~0.35ml, Transparent, Sterile, 48/Case

SPLInsert Standing

SPLInsert™ Standing

SPLInsert Standing transwell inserts (SPL Life Sciences): PC or PET track-etched membrane, 0.4–8µm pore, 6-well (4.06cm²) or 24-well (0.60cm²) format, sterile, DNase/RNase-free. 8 variants, 6 or 12 inserts per plate.

SPLInsert™ Standing, 6 Inserts/6 well plate, PC, 8μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PC, 8μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 0.4μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 3μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 8μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 0.4μm, Growth Area 0.60㎠, 0.2~0.6m, Transparent, Sterile, 48/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 3μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 48/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 8μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 48/Case

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
</li>
<li dir="ltr" aria-level="1">
<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
</li>
</ul>
<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

Media Storage Bottles

<h2 dir="ltr">What are PET Media Bottles?</h2>
 PET (polyethylene terephthalate) media storage bottles are single-use, sterile, optically clear plastic containers for storing tissue culture media, fetal bovine serum, PBS, HBSS, trypsin-EDTA, and other aqueous laboratory solutions. PET is manufactured by an injection stretch-blow molding (ISBM) process that produces glass-like optical clarity, low gas permeability (especially to CO₂ and O₂ compared to polystyrene), excellent mechanical strength, and a smooth inner surface that minimizes liquid residue. PET bottles are BPA-free, non-autoclavable, and rated for use from &minus;60 &deg;C to +60 &deg;C.
&nbsp;They are the dominant single-use format for media and sera storage in cell culture because of their glass-comparable clarity, low extractable profile, and cost-effectiveness compared to borosilicate glass. Choose PET media bottles as the default single-use format for ready-to-use media and sera storage, cold room archiving, and any application where autoclaving is not required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
60mL PET Media Bottles for small-scale sampling
</li>
<li dir="ltr" aria-level="1">
125mL Sterile PET Bottles for routine reagent handling
</li>
<li dir="ltr" aria-level="1">
250mL PET Storage Bottles for mid-range media volumes
</li>
<li dir="ltr" aria-level="1">
500mL/0.5L PET Bottles for standard laboratory preparations
</li>
<li dir="ltr" aria-level="1">
1000mL/1L PET Media Bottles for large-batch stock solutions
</li>
<li dir="ltr" aria-level="1">
2L High-Volume PET Bottles for industrial-scale storage
</li>
<li dir="ltr" aria-level="1">
5L PET Media Vessels for bulk liquid containment
</li>
<li dir="ltr" aria-level="1">
Double-layer Packaged Bottles for enhanced cleanroom safety
</li>
<li dir="ltr" aria-level="1">
Square Shoulder Tray-Packed Bottles for secure, organized transport
</li>
</ul>
<h2 dir="ltr"> How to Choose PET Media Bottles</h2>
 Round vs. Square Format Round PET media bottles (Boston round, GL45) are directly compatible with bottle-top vacuum filtration systems and standard lab bottle accessories. Square PET media bottles (NEST Scientific catalog 334xxx) feature flat sides that stack efficiently in refrigerators and cold rooms, reducing footprint by approximately 25% versus round bottles and preventing rolling when stored horizontally. NEST PET square bottles have molded graduations (not printed), a white labeling area on two sides, and embossed markings for durable identification. Volume Selection PET media bottles are available in 30 mL, 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL volumes. A 500 mL bottle accommodates a standard 500 mL aliquot of cell culture media (DMEM, RPMI, MEM) or a standard FBS lot aliquot. The 1,000 mL bottle holds a full 1 L media preparation. Each bottle includes approximately 15% additional headspace above the nominal volume for thawing expansion (&minus;20 &deg;C frozen FBS thaw) and transfer convenience. Cap Material NEST Scientific PET media bottle caps are made from HDPE (high-density polyethylene), free of heavy metals per regulatory testing. The cap is designed through a one-shot mold process without an inner gasket, fitting securely onto the bottle neck with enhanced sealing performance &mdash; an important feature for leak-proof horizontal storage and air transport. Gas Barrier Properties PET and PETG both reduce gas permeability to CO₂ and O₂ compared to polystyrene, maintaining medium pH and preventing dissolved gas equilibration during storage. For pH-sensitive media containing bicarbonate buffers, PET and PETG are materially superior to polystyrene bottles, which allow CO₂ outgassing and pH shift during storage at room temperature or 4 &deg;C. Sterility and Lot Traceability NEST Scientific PET media bottles are gamma irradiated or E-beam sterilized; each lot is certified DNase/RNase-free, human DNA-free, pyrogen-free, and non-cytotoxic per lot certificate. Lot numbers are printed on each bottle and packaging for traceability. Shelf life is 3 years from the production month per NEST lot release data. PET bottles meet FDA requirements for materials used in contact with sterile liquid pharmaceuticals. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PET square media bottles are manufactured from 100% virgin PET resin via the ISBM process in ISO 8 cleanrooms. Material meets USP Class VI and ISO 10993 biocompatibility, FDA requirements, and USP &lt;661&gt; container testing for plastics in contact with sterile solutions. Operating temperature: &minus;60 &deg;C to +60 &deg;C; not autoclavable. Smooth inner wall minimizes residue; molded graduations on two sides. The HDPE cap is heavy-metal-free. Packaging is double-bagged in sterile format for GMP-compatible cleanroom entry. Shelf life: 3 years from production month. As of 2026, NEST Scientific PET media bottles are stocked by MBP in the full size range. 
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Don&rsquo;t wait for a compromised sample to upgrade your containment. <a href="https://mbpinc.net/contact-us">Contact now </a>and request a quote from Molecular Biology Products Inc. and keep your lab results (and your PI) stress-free.

PET Media Bottles

SQUARE CULTURE MEDIUM BOTTLE, PET, STERILE

Biologix Square Culture Medium Bottles PET Sterile — sterile square PET bottles for cell culture media storage and dispensing. Two variants: 06-1000 (1000 ml) and 06-1500 (500 ml); 24 bottles/bag, 2 bags/case. Available from MBP.

SQUARE CULTURE MEDIUM BOTTLE, 1000ML, PET, STERILE; 24 BOTTLES/BAG, 2 BAGS/CASE

SQUARE CULTURE MEDIUM BOTTLE, 500ML, PET, STERILE; 24 BOTTLES/BAG, 2 BAGS/CASE

STERILE VACUUM FILTRATION UNIT, MEMBRANE: PES

Sterile Vacuum Filtration Unit 09-PES4510 with low-protein-binding PES membrane. Pre-assembled and sterile, ready for media sterilization and filtration of aqueous buffers and biological reagents. Connect to vacuum line or peristaltic pump.

Spheroid Dishes

Spheroid Dish

Spheroid Dishes (110350/111350) by SPL Life Sciences combine a PS dish body with a 200 µm PE mesh well (137 µm thick) for spheroid containment and collection. Sterile to SAL 10⁻⁶; DNase/RNase-free. Two variants: 13 mm untreated well (110350) and 25 mm low-binding treated well (111350). 3/pack, 15/case. For spheroid culture, media exchange, and downstream analysis.