Cell Culture

<h2 dir="ltr"> What are Cell Culture Dishes?</h2>
 Cell culture dishes are sterile, shallow, circular polystyrene containers with a lid for growing adherent or suspension mammalian cells, bacteria, yeast, and microorganisms in a CO₂ incubator or ambient environment. TC-treated (tissue culture&ndash;treated) dishes support adherent mammalian cell monolayer growth; non-treated dishes suit suspension cells, colony counting, and bacteriological applications. Choose the dish diameter based on required cell number and downstream assay volume: 35 mm for small-scale primary culture and microscopy; 60 mm for transfection and mid-scale assays; 100 mm for routine large-scale expansion, protein isolation, and virus production; 150 mm for maximum single-vessel cell yield.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="zaop1j" data-start="0" data-end="134">TC-treated and sterile cell culture dishes in standard, polystyrene (PS), Diamond SureGro, gripping ring, and external grip formats.</li>
<li data-section-id="1tw8fwo" data-start="136" data-end="215">Glass bottom cell culture dishes for microscopy-based cell culture workflows.</li>
<li data-section-id="sr7dgd" data-start="217" data-end="298">Spheroid culture products, including Spheroid Dishes and Spheroid Forming Units.</li>
<li data-section-id="s6nepr" data-start="300" data-end="358">3D Cell Floater Dishes for 3D cell culture applications.</li>
<li data-section-id="4r7o73" data-start="360" data-end="422" data-is-last-node="">Multi-Insert Dishes for multi-insert culture configurations.</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Dishes</h2>
 Diameter and Growth Area by Application 35 mm dishes (growth area approximately 8.8 cm&sup2;, working volume 2 mL) are the standard for primary cell culture, colony counting, clonal selection, and high-resolution phase-contrast microscopy from adherent monolayers, where the small diameter matches the field of view of low-magnification objectives. 60 mm dishes (growth area approximately 21 cm&sup2;, working volume 5 mL) suit transfection experiments (calcium phosphate, lipofection, PEI) and mid-scale protein or RNA harvest. 100 mm dishes (growth area approximately 55 cm&sup2;, working volume 10 mL) are the routine format for cell expansion, large-scale transfection (AAV, lentivirus production), and whole-cell protein or nucleic acid extraction. 150 mm dishes (growth area approximately 148 cm&sup2;, working volume 20&ndash;30 mL) provide maximum cell yield from a single vessel for high-yield protein production, ChIP-seq input material, and large-scale virus production. TC-Treated vs. Non-Treated Dishes TC-treated NEST dishes have vacuum plasma&ndash;modified surfaces (contact angle &lt;10&deg;) supporting adherent mammalian cell attachment, equivalent to TC-treated T-flask surfaces. Non-treated dishes (when available) maintain the hydrophobic PS surface for suspension cell maintenance, organoid culture, and bacteriological applications. Confirm surface treatment from the product label before seeding; TC-treated and non-treated dishes are visually identical. Gripping Ring Option for 100 mm Dishes NEST Scientific 100 mm dishes are available with and without an Easy-Grip gripping loop molded into the dish wall. The gripping ring provides a secure handhold through latex or nitrile gloves in a biosafety cabinet, reducing slip-and-drop contamination events during media changes and trypsin addition for large-batch 100 mm dish workflows. For labs handling &gt;20 dishes per day, gripping ring dishes are a practical quality-of-life improvement. Optical Clarity and Microscopy NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene with a flat, distortion-free growth surface, allowing phase-contrast microscopy and standard fluorescence widefield microscopy directly in the dish without cell transfer. Numeric position marks on the dish bottom allow users to re-locate specific fields between imaging sessions. For confocal, TIRF, or super-resolution imaging requiring No. 1.5 coverglass, use glass-bottom dishes or [glass-bottom cell culture plates](mbpinc.net/consumables/cell-culture/cell-culture-plates/glass-bottom-cell-culture-plates) instead. Stackability and Incubator Efficiency NEST Scientific cell culture dishes feature a raised rim geometry and a snap-fit lid that allow stable stacking in a CO₂ incubator. Stacking feet on the lid prevent the lid from sliding and ensure the inner lid face does not contact the dish rim of the dish below &mdash; maintaining sterility of the lid's inner surface during stacking. For high-throughput cell culture labs running &gt;50 dishes simultaneously, dish stackability significantly increases effective incubator shelf utilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture dishes are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per lot (criterion: contact angle &lt;10&deg;). E-beam sterilized to SAL 10⁻⁶; certified DNase/RNase-free and non-pyrogenic per lot. Flat bottom for distortion-free phase-contrast microscopy; numeric position marks on dish floor. NEST Scientific is ISO 9001:2015, ISO 13485, and ISO 11137 certified with FDA registration and CE marking. As of 2026, NEST Scientific dishes in all four sizes are stocked by MBP. 
&nbsp;
Don&rsquo;t compromise on cultural integrity. <a href="https://mbpinc.net/contact-us">Contact the Molecular Biology Products Inc. team</a> for&nbsp;high-performance labware. Request a quote today to streamline your laboratory workflow.

Cell Culture Dishes

3D Cell Floater Dishes

3D Cell Floater Dish

3D Cell Floater Dishes (26035/26060/26100) by SPL Life Sciences feature a non-TC polystyrene surface that drives gravity-assisted spheroid formation without scaffolds. Available in 35mm (9.4 cm², 3 ml), 60mm (21.5 cm², 5 ml), and 90mm (57.5 cm², 12.5 ml) formats. Sterile; 3-year shelf life at 25 °C. For academic, biopharma, and drug-discovery researchers.

3D Cell Floater Dish, PS, 35X10mm, 9.4㎠, 3ml, external grip, 20/Case

3D Cell Floater Dish, PS, 60X15mm, 21.5㎠, 5ml, external grip, 20/Case

3D Cell Floater Dish, PS, 90X15mm, 57.5㎠, 12.5ml, 20/Case

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
</li>
<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
&nbsp;
<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
&nbsp;
Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

Cell Culture Flasks

3D Cell Floater Flasks

3D Cell Floater Flask with Filter Cap

Biologix 3D Cell Floater Flask, PS, filter cap, low-attachment surface for spheroid culture. 25cm²/7ml (711025, 10/case) and 75cm²/25ml (11-3846, 3/case).

3D Cell Floater Flask with Filter Cap, PS, 25cm2, 10/Case

3D Cell Floater Flask with Filter Cap, PS, 75cm2, 3/Case

<h2 class="p2"> What are Cell Culture Plates?</h2>
 Cell culture plates are sterile, single-use polystyrene multi-well vessels used to grow, treat, and analyze cells in parallel across multiple conditions in a single vessel. All formats are E-beam sterilized to SAL 10⁻⁶, certified DNase/RNase-free and non-pyrogenic, and individually wrapped in peelable film. Choose TC-treated flat-bottom plates for adherent mammalian cell culture, assays, and transfection; choose non-treated plates for suspension, hematopoietic, and hybridoma cells; choose glass-bottom plates for live-cell confocal and high-resolution fluorescence imaging.
&nbsp;
<h2 class="p1">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1rzg2e5" data-start="0" data-end="138"><a href="cell-culture-plates/non-treated-cell-culture-plates">Non-Treated Cell Culture Plates:</a> Designed for suspension cultures and non-adherent cell lines where cell attachment is not required.</li>
<li data-section-id="1f2n3q2" data-start="140" data-end="295"><a href="cell-culture-plates/treated-cell-culture-plates">Treated Cell Culture Plates:</a> Tissue-culture-treated surface promotes reliable attachment and growth of adherent cells for routine culture and assays.</li>
<li data-section-id="1dji5w9" data-start="297" data-end="442" data-is-last-node=""><a href="cell-culture-plates/glass-bottom-cell-culture-plates">Glass Bottom Cell Culture Plates:</a> Glass-bottom wells provide superior optical clarity for high-resolution microscopy and live-cell imaging.</li>
</ul>
<h2 class="p2">&nbsp;</h2>
<h2 class="p2">How to Choose Cell Culture Plates</h2>
 Well Count by Application Scale 6-well plates (9.4 cm&sup2;/well) are the standard for transfection, siRNA knockdown, Western blot sample preparation, luciferase reporter assays, and colony counting &mdash; any protocol that requires &ge;5 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) support medium-scale culture for ELISA lysate preparation, flow cytometry staining, and viral infection studies. 24-well plates (1.9 cm&sup2;/well) are versatile for drug dose-response at medium throughput, primary cell culture, and viral plaque assays. 48-well plates (0.95 cm&sup2;/well) bridge 24-well and 96-well scale for intermediate throughput experiments. 96-well plates (0.32 cm&sup2;/well) are the workhorse for high-throughput cell viability (MTS, MTT, CCK-8), cytotoxicity, ELISA, reporter gene, and compound screening assays. 384-well plates are used for ultra-high-throughput screening (HTS), where reagent cost per well drives miniaturization. TC-Treated vs. Non-Treated vs. Glass Bottom TC-treated (vacuum plasma&ndash;treated, contact angle &lt;10&deg;) flat-bottom plates support adherent mammalian cell monolayer formation &mdash; required for HeLa, HEK293 (adherent), CHO-K1, Vero, A549, MCF-7, and primary cells. Non-treated plates maintain a hydrophobic surface that prevents attachment &mdash; used for suspension cell lines (Jurkat, K562, HL-60), hybridoma culture, and spheroid/organoid formation in U-bottom wells. Glass-bottom plates combine a polystyrene plate body with a borosilicate coverglass floor (0.16&ndash;0.19 mm thickness), matching the No. 1.5 coverglass standard required for confocal, TIRF, and super-resolution microscopy. Flat vs. Round (U) vs. V-Bottom Well Geometry Flat-bottom wells provide a flat imaging surface for phase-contrast and fluorescence microscopy, uniform cell monolayer coverage, and reliable OD measurements in plate readers. Round (U)-bottom wells concentrate cells and particles to the well centre by gravity &mdash; used for rosette assays, hemagglutination inhibition (HAI), and hanging-drop spheroid culture. V-bottom wells have a sharp vertex that pellets cells tightly for wash steps in flow cytometry and immunophenotyping panels. Plate Lid and Evaporation Management NEST Scientific cell culture plates ship with a clear polystyrene lid. For high-throughput assays running 72&ndash;96 hours, edge-well evaporation in 96-well and 384-well formats can introduce artefacts; NEST Low Evaporation EDGE plates have an inter-wall design that reduces edge-well evaporation by maintaining humidity in the peripheral wells during long incubations. 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. TC treatment uses vacuum plasma technology; the lot-release criterion is contact angle &lt;10&deg;. All plates conform to ANSI/SLAS 1-2004 through 4-2004 footprint and well-position standards (SBS format), ensuring compatibility with automated liquid handling platforms, plate readers, and automated incubators. E-beam sterilization to SAL 10⁻⁶. Plates are individually wrapped in peelable Tyvek-equivalent film for GMP cleanroom entry; lids are attached. Alphanumeric well markings (A&ndash;H rows, 1&ndash;12 columns for 96-well) are standard across all formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
Pick your format, culture your cells, and get consistent results every time. <a href="https://mbpinc.net/contact-us">Contact MBP </a>and get a tailored quote today!

Cell Culture Plates

3D Cell Floater Plates

3D Cell Floater Plate

3D Cell Floater Plate — PS ultra-low attachment plates for spheroid formation without special incubation. 6-well (39706), 24-well (39724), and 96-well (39796). For tumor spheroids, organoids, hepatocyte culture, and 3D drug screening.

3D Cell Floater Plate, PS, 6well, 85.4x127.6x20.2mm, 5/Case

3D Cell Floater Plate, PS, 24well, 85.4x127.6x20.2mm, 5/Case

3D Cell Floater Plate, PS, 96well, 85.4x127.6x14.4mm, 5/Case

96well Hanging Drop Plate

96Well Hanging Drop Plate

96 Well Hanging Drop Plates (331196/331296) by SPL Life Sciences enable gravity-assisted spheroid formation in 30 µl drops across a 96-well SBS-footprint (123.6×81.4 mm) format. Hole depth 9.0 mm; PS; sterile. SKU 331196 includes a standard culture plate; 331296 includes a Cell Floater flat plate for continued non-attachment culture. 5/case. For high-throughput 3D drug screening and embryoid body generation.

96Well Hanging Drop Plate with Plate, 5/Case

96Well Hanging Drop Plate Cell Floater, Flat, PS, Hole Depth 9.0mm, Sterile, 5/Case

Biologix Cell Culture Dishes with External Grip

Biologix Laboratory Supplies Cell Culture Series

BIOLOGIX  CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH WITH EXTERNAL GRIP

Biologix Clear PS TC-Treated Sterile Cell Culture Dishes with External Grip — 07-3035 (35×10 mm, 3 ml, 10/pack, 50 packs/case) and 07-3060 (60×15 mm, 5 ml, 10/pack, 50 packs/case). Sterile, TC-treated with external grip for small-scale cell culture and live-cell imaging.

Brand

Model

Material

Dish style DxH(mm)

BIOLOGIX 35X10MM (3ML WORKING VOLUME) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH WITH EXTERNAL GRIP. 10/PACK, 50 PACKS/CASE

BIOLOGIX 60X15MM (5ML WORKING VOLUME) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH WITH EXTERNAL GRIP. 10/PACK, 50 PACKS/CASE

<h2 dir="ltr"> What are Cell Scrapers?</h2>
 Cell scrapers are sterile, single-use disposable tools with a polystyrene handle and a free-rotating polyethylene blade used to mechanically detach and harvest adherent mammalian cells from the TC-treated surface of culture flasks, dishes, and multi-well plates when enzymatic detachment (trypsin, Accutase, Dispase) is not appropriate. The free-rotating blade mechanism allows the angle to be adjusted by applying gentle pressure on the handle &mdash; the blade twists to align with the vessel surface for consistent, complete cell removal across the full growth area. Choose the 13 mm blade for T25 and T75 flasks and multi-well plates; choose the 20 mm blade for T175, T225, and 100&ndash;150 mm dishes, where a wider blade reduces the number of passes needed to clear the full growth surface.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Cell Scrapers with 18cm Handle for compact culture vessels</li>
<li dir="ltr" role="presentation">Cell Scrapers with 24.3cm Handle for extended reach in flasks</li>
<li dir="ltr" role="presentation">220mm Handle Scrapers with 13mm Blade for precision harvesting</li>
<li dir="ltr" role="presentation">280mm Handle Scrapers with 20mm Blade for large-surface area recovery</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Scrapers</h2>
 When to Use Cell Scrapers Instead of Trypsin Cell scraping is the correct harvesting method when:
<ol>
<li style="text-align: left;">Trypsinization damages the protein or surface marker of interest; scraping preserves surface antigens, including CD markers for flow cytometry and receptor proteins for biochemical assays.&nbsp;</li>
<li style="text-align: left;">The cell line does not detach effectively with trypsin (some primary cells, fibroblasts in dense monolayers, epithelial cells after extended culture)</li>
<li style="text-align: left;">Harvest must be complete and rapid for protein or RNA extraction protocols with RNase-sensitive targets, where trypsin incubation time introduces RNA degradation risk</li>
<li style="text-align: left;">The downstream assay uses cells in medium without trypsin carryover (some enzyme assays, reporter gene assays, and cell viability assays where trypsin activity in the sample affects results). </li>
</ol>
Blade Size by Vessel Format The 13 mm blade (catalog 710001, 220 mm handle) is designed for T25 and T75 flasks and multi-well plates (6-well through 24-well). The blade width covers approximately 1&ndash;1.5 passes across a T75 flask width for complete surface coverage. The 20 mm blade (catalog 710011, 280 mm handle) suits T175 and T225 flasks, 100 mm and 150 mm dishes, and any large-surface vessel where the wider blade reduces scraping passes needed and operator fatigue. Both blade sizes fit through the standard T-flask neck opening. Free-Rotating Blade Mechanism NEST Scientific cell scrapers use a free-rotating blade that pivots relative to the handle on a hinge mechanism. Applying gentle lateral pressure on the handle adjusts the blade angle, allowing it to maintain flush contact with the vessel floor during the scraping stroke &mdash; improving cell detachment yield compared to fixed-blade scrapers, where the blade angle may not match the vessel floor geometry. After the push stroke, rotating the handle slightly returns the blade to the correct alignment for the next stroke. Scraping Technique Add 1&ndash;2 mL of culture medium or PBS to the vessel before scraping to lubricate the surface and prevent cells from drying. Work systematically across the flask or dish in parallel overlapping strokes. For T-flasks, tilt the flask at 20&ndash;30&deg; to pool the cell suspension at the low end after scraping, then pipette out the suspension. Do not press excessively &mdash; scraping force sufficient to detach cells should not require grinding the blade into the vessel floor, which damages cells and the scraper. 
<h2 style="text-align: left;"> Specifications Context</h2>
 NEST Scientific cell scrapers (catalog 710001 and 710011) are manufactured with a polystyrene handle and polyethylene blade. E-beam sterilized per ANSI/AAMI/ISO 11137 to SAL 10⁻⁶; individually wrapped in easy-tear sterile packaging; non-pyrogenic, DNase/RNase-free per lot certificate. Performance testing is conducted on each manufacturing lot per NEST Scientific standard operating procedures. NEST Scientific cell scrapers are available through MBP in cases of 100 (1/pk &times; 100/cs). The free-rotating blade design is a confirmed feature per NEST Scientific USA product documentation. 
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the team at Molecular Biology Products Inc.</a> to request a quote for your laboratory supply needs.

Cell Scrapers

BIOLOGIX CELL SCRAPERS

Biologix Cell Scrapers (70-1180 / 70-1250): polyethylene, pivoting 18 mm blade, sterile, individually wrapped. 180 mm handle (100/case) for standard flasks; 250 mm handle (200/case) for deeper vessels. Enzyme-free adherent cell detachment.

BIOLOGIX DISPOSABLE POLYETHYLENE STERILE CELL SCRAPER (HANDLE LENGTH: 180MM, BLADE LENGTH: 18MM). SCRAPERS COME INDIVIDUALLY WRAPPED.  100/CASE

BIOLOGIX DISPOSABLE POLYETHYLENE STERILE CELL SCRAPER (HANDLE LENGTH: 250MM, BLADE LENGTH: 18MM). SCRAPERS COME INDIVIDUALLY WRAPPED.  200/CASE

Biologix Cell Culture Dishes without External Grip

Biologix® Cell Culture Dishes-150x25mm

BIOLOGIX CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH

07-31XX Biologix Clear PS TC-Treated Sterile Cell Culture Dishes — 07-3100 (90×20 mm, 12.5 ml, 10/pack, 20 packs/case) and 07-3150 (150×25 mm, 35 ml, 10/pack, 12 packs/case). Sterile, TC-treated polystyrene for standard adherent cell culture.

BIOLOGIX 90X20MM (12.5ML WORKING VOLUME) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH. 10/PACK, 20 PACKS/CASE

BIOLOGIX 150X25MM (35ML WORKING VOLUME) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE DISH. 10/PACK, 12 PACKS/CASE

<h2 class="p2">What are TC-Treated Cell Culture Plates?</h2>
 TC-treated (tissue culture&ndash;treated) cell culture plates are sterile, single-use multi-well polystyrene plates whose well surfaces have been modified by vacuum plasma treatment to introduce negatively charged oxygen-containing groups (carboxyl, carbonyl, hydroxyl), reducing the water contact angle from ~75&ndash;90&deg; (untreated PS) to below 10&deg; (hydrophilic). This surface modification directly enables attachment, spreading, and monolayer formation of adherent mammalian cell lines. TC-treated plates are the default format for all adherent mammalian cultures, cell-based assays, ELISA preparation, compound cytotoxicity screening, transfection, and gene expression studies.&nbsp; 
<h2 class="p1">What you will find:</h2>
&nbsp;
&bull; 6, 12, 24, 48, and 96 well formats for different workflows &bull; Tissue-culture-treated surfaces for reliable cell attachment &bull; Clear plates for easy monitoring under the microscope &bull; Uniform wells for consistent seeding and growth &bull; Sterile options to maintain clean conditions &bull; Lids designed to reduce contamination and evaporation
&nbsp;
<h2 class="p2">How to Choose TC-Treated Cell Culture Plates</h2>
 Well Format by Cell Number and Assay Type 6-well TC-treated plates (9.4 cm&sup2;/well) are the standard for Western blot preparation, siRNA transfection, viral infection, protein overexpression, and colony-forming unit (CFU) assays requiring &ge;3 &times; 10⁶ cells per condition. 12-well plates (3.8 cm&sup2;/well) suit medium-scale reporter assays, luciferase quantification, and ELISA from cell lysate. 24-well plates (1.9 cm&sup2;/well) are versatile for cytotoxicity dose-response in 4&ndash;6 conditions per plate, primary cell experiments, and viral plaque assays. 96-well flat-bottom TC-treated plates (0.32 cm&sup2;/well) are the universal format for cell viability (MTS, MTT, CCK-8), compound screening, ELISA, reporter gene assays, and qPCR lysate preparation across 8&ndash;12 concentrations with triplicate wells. 384-well plates reduce reagent cost per compound for HTS drug discovery programs. Lot-to-Lot TC Treatment Consistency For longitudinal drug screening, biomarker validation, or multi-site studies, lot-to-lot variation in TC treatment can alter cell attachment density, proliferation rate, and assay Z-factor. NEST Scientific verifies vacuum plasma treatment by water contact angle goniometry on each manufacturing lot; the release criterion is contact angle &lt;10&deg;. Request lot certificates when adopting TC-treated plates for GLP- or GMP-regulated assay workflows. Low-Evaporation EDGE Plates For 96-well dose-response assays running 72&ndash;96 hours, peripheral (edge) well evaporation can increase medium osmolality in the outer ring by 10&ndash;20% versus inner wells, introducing a systematic bias in IC50 determinations. NEST Low Evaporation EDGE 96-well TC-treated plates use an internal well ridge design that reduces edge-well evaporation during extended incubations. Use EDGE plates for any dose-response study where incubation time exceeds 48 hours. Cell Line Compatibility TC-treated NEST plates support attachment and monolayer culture of: HeLa (cervical), HEK293 (adherent), A549 (lung), MCF-7 (breast), U2OS (bone), CHO-K1 (ovary, adherent), Vero (kidney), MDCK (canine kidney), NIH 3T3 (mouse fibroblast), MRC-5, 2BS, 293T, L-929, and standard primary cultures including dermal fibroblasts, endothelial cells, and epithelial cells. Do NOT use TC-treated plates for Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9, Hi5, or any non-adherent cell line. Plate Reader and Automation Compatibility NEST Scientific TC-treated 96-well flat plates are compatible with all common microplate readers (BioTek Synergy H1, Molecular Devices SpectraMax i3x, PerkinElmer EnVision, BMG Labtech CLARIOstar) for absorbance, fluorescence intensity, and luminescence detection. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS dimensions for automated liquid handlers (Tecan Fluent, Hamilton STAR, Beckman Coulter Biomek i7). 
<h2 class="p2"> Specifications Context</h2>
 NEST Scientific TC-treated cell culture plates are manufactured from 100% virgin polystyrene in ISO 8 cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry; the lot release criterion: contact angle &lt;10&deg;. Plates conform to ANSI/SLAS 1-2004 through 4-2004 SBS footprint. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free, non-pyrogenic per lot certificate. Clear polystyrene lids included; individually wrapped in peelable film. Alphanumeric well coordinates on all &ge;12-well formats. NEST Scientific is ISO 9001:2015 and ISO 13485 certified with FDA registration. As of 2026, NEST Scientific TC-treated plates across all core well formats (6- through 384-well) are stocked by MBP. 
Use treated plates when attachment drives your results. <a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to get reliable plates for keeping your cells anchored, healthy, and consistent across every well.&nbsp;

Treated Cell Culture Plates

BIOLOGIX CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS

Biologix TC-Treated Sterile Cell Culture Plates with Lids — 07-6006 (6-well, 3 ml), 07-6012 (12-well, 2 ml), 07-6024 (24-well, 1 ml), 07-6048 (48-well, 0.5 ml), 07-6096 (96-well, 0.2 ml); clear PS; individually wrapped; 50/case. Standard footprint, TC-treated, with lid.

Wells

BIOLOGIX 6-WELL (EXTERNAL DIMENSIONS: 85.4MM(W)X127.6MM(L)X20.2MM(H) | WELL DIMESIONS: 35MM(D)X17.5MM(H) | WORKING VOLUME: 3ML) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS. PLATES COME INDIVIDUALLY WRAPPED.  50/CASE

BIOLOGIX 12-WELL (EXTERNAL DIMENSIONS: 85.4MM(W)X127.6MM(L)X20.2MM(H) | WELL DIMESIONS: 21.9MM(D)X17.5MM(H) | WORKING VOLUME: 2ML) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS. PLATES COME INDIVIDUALLY WRAPPED.  50/CASE

BIOLOGIX 24-WELL (EXTERNAL DIMENSIONS: 85.4MM(W)X127.6MM(L)X20.2MM(H) | WELL DIMESIONS: 15.5MM(D)X17.5MM(H) | WORKING VOLUME: 1ML) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS. PLATES COME INDIVIDUALLY WRAPPED.  50/CASE

BIOLOGIX 48-WELL (EXTERNAL DIMENSIONS: 85.4MM(W)X127.6MM(L)X20.2MM(H) | WELL DIMESIONS: 9.75MM(D)X17.5MM(H) | WORKING VOLUME: 0.5ML) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS. PLATES COME INDIVIDUALLY WRAPPED.  50/CASE

BIOLOGIX 96-WELL (EXTERNAL DIMENSIONS: 85.4MM(W)X127.6MM(L)X14.4MM(H) | WELL DIMESIONS: 6.5MM(D)X10.8MM(H) | WORKING VOLUME: 0.2ML) CLEAR POLYSTYRENE (TC-TREATED) STERILE CELL CULTURE PLATES WITH LIDS. PLATES COME INDIVIDUALLY WRAPPED.  50/CASE

<h2 dir="ltr">What are Syringe Filters?</h2>
 Syringe filters are self-contained, disposable filtration units comprising a polypropylene (PP) housing enclosing a flat-sheet membrane, with a female Luer lock inlet and a male Luer lock outlet. They are attached to a standard syringe, the sample is drawn up, and pushed through the membrane by manual plunger pressure. Standard disc diameters are 4 mm, 13 mm, 25 mm, and 33 mm; membrane pore sizes are 0.22 &micro;m (sterilizing grade) and 0.45 &micro;m (clarification grade), with 0.10 &micro;m available for mycoplasma removal. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 25 mm filter &asymp; 2.98 cm&sup2;. Globe Scientific syringe filters, a confirmed MBP brand, are available in PP housing with PES, PVDF, CA (cellulose acetate), MCE, and nylon membranes in 13 mm and 30 mm diameters, sterile (individually wrapped) and non-sterile (bulk), 0.22 &micro;m and 0.45 &micro;m. Choose syringe filters for volumes of 1&ndash;150 mL; choose vacuum bottle-top filters for volumes above 150 mL.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
PES Syringe Filters for high-flow aqueous filtration
</li>
<li dir="ltr" aria-level="1">
PVDF Syringe Filters for low protein binding
</li>
<li dir="ltr" aria-level="1">
Nylon Syringe Filters for organic solvent compatibility
</li>
<li dir="ltr" aria-level="1">
0.22&mu;m Syringe Filters for absolute sterilization
</li>
<li dir="ltr" aria-level="1">
0.45&mu;m Syringe Filters for clarification and pre-filtration
</li>
<li dir="ltr" aria-level="1">
PTFE Syringe Filters for aggressive chemical resistance
</li>
<li dir="ltr" aria-level="1">
Hydrophilic Syringe Filters for versatile liquid handling
</li>
<li dir="ltr" aria-level="1">
Sterile Individually Blistered Filters for aseptic security
</li>
</ul>
<h2 dir="ltr"> How to Choose Syringe Filters</h2>
 Diameter Selection by Sample Volume 13 mm syringe filters (effective area 0.92 cm&sup2;) handle up to 10&ndash;15 mL per filter before flow resistance becomes impractical. Use with 1&ndash;10 mL syringes. 25 mm syringe filters (2.98 cm&sup2;) handle 10&ndash;100 mL per filter. Use with 10&ndash;60 mL syringes. 33 mm syringe filters (approximately 5&ndash;6 cm&sup2;) handle up to 150 mL and are the largest practical syringe filter format. For volumes above 150 mL, switch to a vacuum bottle-top filter system. Matching filter diameter to sample volume prevents premature membrane clogging and excessive plunger force. Membrane Chemistry for Syringe Filters PES (Polyethersulfone): Low protein binding, high flow rate, aqueous compatibility. Standard choice for biological sample sterilization, cell culture supplement filtration (antibiotics, glutamine, growth factors), and conditioned medium aliquots. Not suitable for strong organic solvents or concentrated acids. PVDF (Polyvinylidene fluoride, hydrophilic): Low protein binding &mdash; among the lowest of all syringe filter membranes, with protein recovery rates &gt;90&ndash;99.9% documented for antibody and enzyme solutions. Compatible with mild organic solvents, weak acids, and most aqueous solutions. The first choice when filtering precious protein solutions (antibodies, enzymes, viral preparations) is where maximum protein recovery is critical. Not suitable for pure DMSO (use PTFE). Nylon (Polyamide): Low extractables, broad chemical compatibility including mixed aqueous-organic solvents, methanol, acetonitrile, and DMSO-water mixtures. Standard for HPLC mobile phase filtration and dissolution testing. Higher protein binding than PES or PVDF &mdash; avoid for biological samples where protein loss is a concern. MCE (Mixed Cellulose Ester): Low cost, aqueous applications only; moderate protein binding. Suitable for general buffer preparation, water sterilization, and non-critical sample preparation. Not compatible with organic solvents or strong acids. CA (Cellulose Acetate): Low protein binding, similar to PES. Suited for biological sample filtration and sterility testing applications. Contains surfactant treatment for wettability &mdash; check for extractable compatibility in downstream assays. PTFE (Polytetrafluoroethylene, hydrophilic): Maximum chemical resistance &mdash; compatible with pure DMSO, concentrated acids and bases, and aggressive organic solvents. The only membrane choice for 100% organic solvent filtration. For aqueous solutions, use hydrophilic PTFE (pre-wetted with methanol or IPA before aqueous filtration unless the hydrophilic version is selected). Sterile vs. Non-Sterile Syringe Filters Sterile syringe filters (individually gamma-irradiated or E-beam sterilized, individually packaged) are required for cell culture media sterilization, supplement preparation, and sample preparation for biological culture. Non-sterile bulk-packed filters (Globe Scientific non-sterile formats) are appropriate for HPLC mobile phase preparation, analytical sample clarification, and non-biological applications where sterility of the filtrate is not required. 
<h2 dir="ltr">Specifications Context</h2>
 Globe Scientific syringe filters are manufactured in a PP housing with a luer-lock inlet and outlet conforming to ISO 594 / ISO 80369 for Luer fittings. PES and PVDF sterile filters are individually gamma irradiated; each is separately wrapped and printed with an expiry date per Globe Scientific lot protocol. Effective filtration areas: 13 mm filter &asymp; 0.92 cm&sup2;; 30 mm filter &asymp; 4.91 cm&sup2; (Globe Scientific welded format). Operating temperature: up to 60 &deg;C. Do not exceed the maximum operating pressure of 6 bar (87 psi). Globe Scientific is ISO 9001:2015 certified. Globe Scientific syringe filters in PES, PVDF, CA, MCE, and nylon membranes at 13 mm and 30 mm diameters are stocked by MBP. 
Request a quote today for premium syringe filters from <a href="https://mbpinc.net/contact-us">the MBP team</a> to&nbsp;find the right filtration solution for your laboratory applications.

Syringe Filters

Membrane: PES, pore size: 0.22μm, diameter: 13mm. Female luer lock + male luer slip. Individually packed.

Membrane: PES, pore size: 0.22μm, diameter: 25mm. Female luer lock + male luer slip. Individually packed.

Membrane: PES, pore size: 0.22μm, diameter: 30mm. Female luer lock + male luer slip. Individually packed.

Membrane: PES, pore size: 0.22μm, diameter: 33mm. Double luer lock. Individually packed.

Membrane: PES, pore size: 0.45μm, diameter: 33mm. Double luer lock. Individually packed.

Membrane: PES, pore size: 0.22μm, diameter: 33mm. Female luer lock + male luer slip. Individually packed.

Membrane: PES, pore size: 0.45μm, diameter: 33mm. Female luer lock + male luer slip. Individually packed.

Membrane: CA, pore size: 0.22μm, diameter: 30mm. Female luer lock + male luer slip. Individually packed.

Membrane: CA, pore size: 0.45μm, diameter: 30mm. Female luer lock + male luer slip. Individually packed.

Membrane Specifications

BIOLOGIX STERILE SYRINGE FILTER, MEMBRANE: PES

08-PES1322R Biologix Sterile PES Syringe Filter — 0.22 µm; 13 mm diameter (smallest format, lowest hold-up volume); sterile; individually packed. Ideal for small-volume (1–10 ml) sterile filtration of precious biological samples.

BIOLOGIX STERILE SYRINGE FILTER, MEMBRANE: PES,PORE SIZE: 0.22ΜM,DIAMETER:33MM. FEMALE LUER LOCK + MALE LUER SLIP. INDIVIDUALLY PACKED, 100 PCS/PACK, 4 PACKS/CASE

08-PES3322M Biologix Sterile PES Syringe Filter — 0.22 µm; 33 mm diameter; female Luer lock + male Luer slip; individually packed; 100/pack, 4 packs/case (400/case). High-capacity 33 mm format for larger-volume sterile syringe filtration of cell culture media.

BIOLOGIX STERILE SYRINGE FILTER, MEMBRANE: PES,PORE SIZE: 0.45ΜM,DIAMETER:33MM. FEMALE LUER LOCK + MALE LUER SLIP. INDIVIDUALLY PACKED, 100 PCS/PACK, PACKS/CASE

08-PES3345M Biologix Sterile PES Syringe Filter — 0.45 µm; 33 mm diameter; female Luer lock + male Luer slip; individually packed; 100/pack. For clarification filtration, pre-filtration, and particulate removal from biological solutions.

Biologix Cell Culture Plate, TC Treated, PS, Flat-Bottom

Biologix PS TC-treated flat-bottom cell culture plates — individually wrapped sterile. 6-well (30006), 12-well (30012), 48-well (30048), 96-well (30096). SBS footprint 85.4×127.6 mm. For adherent mammalian cell culture and HTS assays.