Azura Genomics

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

User Manual

Product Literature

MSDS

AzuraQuant Probe Fast qPCR Mix LoRox

AZ-2801 by Azura Genomics: AzuraQuant™ Probe Fast qPCR Mix LoRox — 2× master mix for TaqMan, Scorpions, and molecular beacon probe assays on standard and fast cycling instruments. Low ROX passive reference. Antibody hot-start. 100–10,000 rxn packs.

Manufacturer

AzuraQuant Probe Fast qPCR Mix LoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 500 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Probe Fast qPCR Mix LoRox 5,000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox

AZ-2901 by Azura Genomics is a 2x ready-to-use probe-based qPCR master mix formulated without ROX passive reference dye. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Designed for instruments that do not require ROX normalization, including Bio-Rad CFX, Roche LightCycler, and Qiagen Rotor-Gene. Available in 100 to 10,000 reaction formats.

AzuraQuant Probe Fast qPCR Mix NoRox 100 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 500 Reactions

AzuraQuant Probe Fast qPCR Mix NoRox 2000 reactions

AzuraQuant Probe Fast qPCR Mix NoRox 5000 reactions

SYBR/Dye-Based

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

AzuraQuant Green 1-Step qPCR Mix - Product Sheet

AzuraQuant™ Green 1-Step qPCR Mix HiRox

AZ-3501 by Azura Genomics — AzuraQuant™ Green 1-Step qPCR Mix HiRox, 2× master mix + 20× AzuraSprint RT for one-step RT-qPCR directly from RNA. SYBR Green channel, HiRox for Applied Biosystems instruments. 20 µL reactions, −20°C storage, 18-month shelf life. Available 100–10,000 reaction packs.

AzuraQuant Green 1-Step qRT-PCR Kit Hi-Rox 100 Reactions

AzuraQuant Green 1-Step qPCR Mix HiRox 500 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Green 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant™ Green 1-Step qPCR Mix LoRox

AZ-3701 by Azura Genomics is a 2× one-step RT-qPCR master mix (low ROX) with a companion 20× thermostable AzuraSprint reverse transcriptase and non-inhibitory intercalating dye, enabling single-tube RNA quantification with post-amplification melt curve analysis on low-ROX compatible instruments.

AzuraQuant Green 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 500 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 1000 Reactions

AzuraQuant Green 1-Step qPCR Mix LoRox 5000 Reactions

AzuraQuant Green Fast qPCR Mix HiRox

AzuraQuant Green Fast qPCR Mix

AzuraQuant™ Green Fast qPCR Mix HiRox

AzuraQuant™ Green Fast qPCR Mix HiRox (AZ-2001) by Azura Genomics is a 2× hot-start SYBR-channel qPCR master mix with a high-ROX passive reference dye, optimised for Applied Biosystems and QuantStudio platforms. Compatible with standard and fast cycling protocols. Available in 100, 500, 2,000, and 5,000 reaction sizes from MBP Inc.

AzuraQuant Green Fast qPCR Mix HiRox, 100 reactions

AzuraQuant Green Fast qPCR Mix HiRox 500 Reactions

AzuraQuant Green Fast qPCR Mix HiRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix HiRox 5,000 reactions

AzuraQuant Green Fast qPCR Mix - Product Sheet

qPCR Reagent Compatibility Tool

AzuraQuant™ Green Fast qPCR Mix LoRox

AZ-2101 by Azura Genomics — AzuraQuant™ Green Fast qPCR Mix LoRox, 2× master mix with Azura HS Taq, SYBR Green channel, low ROX. 20 µL standard reactions, fast-cycling protocol (95°C 2 min / 5 sec denaturation / 20–30 sec anneal-extension). Stored at −20°C, 18-month shelf life. Available in 100–5,000 reaction packs.

AzuraQuant Green Fast qPCR Mix LoRox 100 reactions

AzuraQuant Green Fast qPCR Mix LoRox 500 Reactions

AzuraQuant Green Fast qPCR Mix LoRox 2,000 reactions

AzuraQuant Green Fast qPCR Mix LoRox 5000 reactions

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

AzuraQuant™ II cDNA Synthesis Kit

AZ-2501 by Azura Genomics is a 2-tube first-strand cDNA synthesis kit built on HighTherm™ II reverse transcriptase. Converts 4 pg to 2 µg total RNA to cDNA in 25 minutes using a combined random hexamer and anchored oligo(dT) strategy. Available in 25, 100, and 500 reaction formats for 2-step RT-qPCR and RT-PCR workflows.

AzuraQuant II cDNA Synthesis Kit 25 Reactions

AzuraQuant II cDNA Synthesis Kit 100 Reactions

AzuraQuant II cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit - Product Sheet

AzuraQuant™ cDNA Synthesis Kit

AZ-1995 by Azura Genomics is the AzuraQuant™ cDNA Synthesis Kit for 2-step RT-qPCR and RT-PCR. Two-tube format: 5x Buffer and 20x HighTherm™ RT with RNase inhibitor, plus random hexamer and anchored oligo(dT) primers. RNA input from 4 pg. Available in 25, 100, and 500 reaction sizes.

AzuraQuant cDNA Synthesis Kit 25 Reactions

AzuraQuant cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit 100 reactions

AzuraView™ GreenFast qPCR Blue Mix HR

AZ-2401 by Azura Genomics — AzuraView GreenFast qPCR Blue Mix HR; 2X SYBR Green master mix; Azura HS Taq; inert blue tracer dye; increased resistance to freeze-thaw; compatible with all standard qPCR instruments; 100 reactions (1×1 mL).

AzuraView GreenFast qPCR Blue Mix HR 100 Reactions

AzuraView GreenFast qPCR Blue Mix HR 500 Reactions

AzuraView GreenFast qPCR Blue Mix HR 2,000 Reactions

AzuraView GreenFast qPCR Blue Mix HR 5,000 Reactions

AzuraView GreenFast qPCR Blue Mix - Product Sheet

AzuraView™ GreenFast qPCR Blue Mix LR

AzuraView™ GreenFast qPCR Blue Mix LR (AZ-2301) by Azura Genomics — 2× ultra-sensitive intercalating dye master mix with Azura HS Taq, blue tracer dye, low ROX. Standard and fast cycling compatible, stable through 40 freeze-thaw cycles. Available from 100 to 10,000 reactions.

AzuraView GreenFast qPCR Blue Mix LR 100 Reactions

AzuraView GreenFast qPCR Blue Mix LR 500 Reactions

AzuraView GreenFast qPCR Blue Mix LR 2000 Reactions

AzuraView GreenFast qPCR Blue Mix LR 5,000 Reactions

AzuraView Probe Fast qPCR Mix Blue - Product Sheet

AzuraView™ Probe Fast qPCR Mix Blue

AZ-2601B by Azura Genomics is an inhibitor-tolerant 2× master mix formulated for probe-based real-time qPCR, including TaqMan® and molecular beacon assays, with a blue inert tracer dye for visual confirmation of pipetting and compatibility with standard and fast-cycling thermal cyclers.

AzuraView Probe Fast qPCR Mix Blue 100 reactions

AzuraView Probe Fast qPCR Mix Colorless 100 reactions

AzuraView Probe Fast qPCR Mix Blue 500 reactions

AzuraView Probe Fast qPCR Mix Colorless 500 reactions

AzuraView Probe Fast qPCR Mix Blue 2000 reactions

AzuraView Probe Fast qPCR Mix Colorless 2000 reactions

AzuraView Probe Fast qPCR Mix Blue 5000 reactions

AzuraView Probe Fast qPCR Mix Colorless 5000 reactions

Direct PCR Master Mixes

<h2>What Are Direct PCR Master Mixes?</h2>
&nbsp;
Direct PCR master mixes are specially formulated 2x PCR reagents designed to amplify nucleic acid targets directly from crude biological samples such as blood, buccal swabs, tissue punches, cultured cells, or other minimally processed material without a separate DNA or RNA extraction step. This is achieved through inhibitor-tolerant polymerase formulations, often combined with proprietary buffer additives that counteract the effects of hemoglobin, melanin, polysaccharides, and other common PCR inhibitors present in crude lysates.&nbsp;Choose a direct PCR master mix for high-throughput genotyping or rapid screening applications where eliminating the extraction step provides meaningful time and cost savings, provided the application tolerates the typically modest reduction in amplification efficiency compared to extracted-template PCR.
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<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Direct PCR &amp; Fast Extraction Kits: Streamlined solutions such as the Flash-Extract&trade; PCR Kit and Accuris Fast Extraction PCR Kit that enable rapid PCR workflows from minimally processed samples, reducing preparation time and simplifying genotyping applications.</li>
<li>Genotyping Kits: Specialized products including the Azura&trade; Mouse Genotyping Kit and Accuris 1 Hour Mammalian Genotyping Kit, designed for reliable genotyping workflows using tissue, tail, ear, or other biological samples.</li>
</ul>
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<h2>How to Choose a Direct PCR Master Mix</h2>
&nbsp;
Sample Type Compatibility
Direct PCR formulations vary in the crude sample types they are validated for. Blood, tissue, cells, and swabs each present different inhibitor profiles, so confirm the master mix is validated for your specific sample type before adopting it for a new workflow.
Inhibitor Tolerance Mechanism
Inhibitor tolerance is typically achieved through a combination of enzyme engineering and buffer additives that bind or neutralize inhibitory compounds. The specific combination affects which inhibitor types, such as heme from blood or melanin from pigmented tissue, the mix is best suited to handle.
Lysis Step Requirements
Some direct PCR master mixes require a brief lysis step, such as a short heat or detergent treatment, before amplification, while others are formulated for direct addition of the crude sample to the PCR reaction. Confirm the workflow complexity matches your throughput needs.
Amplicon Size and Application
Direct PCR is well established for short-to-moderate amplicon genotyping and screening applications. Very long amplicons or applications requiring maximum yield from limited template may still benefit from a brief extraction step even when a direct-compatible mix is available.
Comparison to Extraction-Based PCR
Direct PCR master mixes generally show good performance for routine genotyping but may exhibit somewhat reduced sensitivity or yield compared to PCR from purified template. For applications where extraction-free convenience outweighs a modest performance trade-off, direct PCR offers substantial time savings, particularly at high sample throughput.
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<h2>Specifications Context</h2>
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Direct PCR master mixes enable PCR directly from crude samples through heat-stable polymerases tolerant of inhibitor compounds and lysis buffer components, combined with optimized buffer systems designed to neutralize hemoglobin, melanin, polysaccharides, and other inhibitors commonly found in blood, tissue, and plant material. The time savings from eliminating extraction are most significant in high-throughput genotyping pipelines, where extraction across hundreds of samples can represent a substantial fraction of total workflow time. As with any inhibitor-tolerant formulation, performance can vary by specific sample matrix. Validating a direct PCR master mix against a small panel of representative samples before deploying it across a full study is considered good practice. Direct PCR master mixes are typically stored at -20&deg;C. Lot-specific Certificates of Analysis (CoAs) are available on request.&nbsp;
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Streamline your amplification workflow&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional Direct PCR solutions.

Mouse Genotyping Kit - Product Sheet

Azura™ Mouse Genotyping Kit

AZ-1851 by Azura Genomics: mouse genotyping kit combining rapid lysis buffer (15-min extraction, no columns) with Azura 2× HS Red Mix for direct gel loading. Full workflow in ~1 hour. Available in 80-, 400-, and 2,000-reaction packs.