Azura Genomics

Taq PCR Master Mixes

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
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Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

User Manual

Azura 2x Taq Mix - Product Sheet

MSDS

Azura Taq Master Mix

AZ-1302 by Azura Genomics is a 2x ready-to-use Taq DNA polymerase master mix containing Taq polymerase, a proprietary PCR enhancer, and pre-optimized dNTPs and MgCl2. Formulated for high-throughput PCR, genotyping, colony PCR, and TA cloning. Available in 200-reaction (4×1.25 mL), 1000-reaction, and 2000-reaction formats.

Manufacturer

Azura 2x Taq Mix 200 reactions

Azura 2x Taq Mix 1000 reactions

Azura 2x Taq Mix 2000 reactions

High-Fidelity DNA Polymerase

<h2>What Are High-Fidelity DNA Polymerases?</h2>
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The development of high-fidelity thermostable DNA polymerases transformed PCR from a tool for detection and amplification into a precise DNA engineering platform. By combining thermostability with 3' to 5' proofreading exonuclease activity, modern high-fidelity enzymes detect and remove misincorporated bases immediately after insertion during DNA synthesis. Polymerase fidelity can significantly affect downstream results, so choosing an enzyme that meets your accuracy and performance requirements is essential.
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<h2>What you will find:</h2>
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<ul>
<li>High-fidelity DNA amplification with proofreading activity using Azura TruFi&trade; II DNA Polymerase.</li>
<li>Proofreading polymerase formulations with matched reaction buffers, including ExtremeTaq&trade; DNA Polymerase with 5x Reaction Buffer.</li>
<li>Accurate PCR amplification with Accuris High Fidelity DNA Polymerase for cloning, sequencing, and mutagenesis workflows.</li>
<li>High-fidelity enzyme systems featuring proofreading activity, including MegaFi Pro Fidelity DNA Polymerase.</li>
</ul>
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<h2>How to Choose a High-Fidelity DNA Polymerase</h2>
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Enzyme Family Selection
High-fidelity polymerases differ in processivity, extension speed, fidelity, and template compatibility. Selection should consider amplicon length, GC content, template complexity, and downstream application requirements.
Format: Enzyme vs. Master Mix
Individual high-fidelity enzymes with optimized HF buffers suit custom reaction optimization, particularly for difficult templates requiring buffer additive titration. In contrast, 2x master mixes streamline routine high-fidelity amplification for cloning and mutagenesis workflows.
Error Rate Requirements
For applications where amplicons will be cloned and sequence-verified downstream, the approximately 50- to 100-fold lower error rate of high-fidelity enzymes compared with standard Taq polymerase substantially reduces the frequency of sequence-confirmed clones carrying PCR-introduced mutations.
Extension Speed and Cycling Time
High-fidelity polymerases often extend faster than standard Taq per kilobase, which can shorten overall cycling time for long amplicons. However, optimal cycling parameters are enzyme-specific and should follow the manufacturer's recommendations.
Cloning Method Compatibility
Some high-fidelity polymerases generate blunt-ended PCR products suited to Gibson Assembly or blunt-end cloning strategies, while others may require additional steps for TA cloning. Confirm compatibility with your intended downstream cloning method before selection.
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<h2>Specifications Context</h2>
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The development of high-fidelity thermostable DNA polymerases&mdash;combining thermostability with 3' to 5' proofreading exonuclease activity&mdash;transformed PCR from a detection and amplification tool into a precise DNA engineering platform, enabling reliable cloning, site-directed mutagenesis, and sequencing-grade amplification. The proofreading mechanism corrects misincorporated bases immediately after insertion, reducing error rates to approximately 10&minus;6 per nucleotide. Selection between high-fidelity polymerases often depends on template characteristics such as GC content, amplicon length, secondary structure, and downstream cloning requirements. High-fidelity enzymes are typically stored at &minus;20 &deg;C with matched HF buffer systems, and lot-specific Certificates of Analysis (CoAs) are available upon request. For sequencing-bound amplicons, confirm that the selected polymerase's documented error rate meets the level of sequence accuracy required, as even a 50- to 100-fold improvement over standard Taq may not be sufficient for applications demanding extremely low mutation rates across very long amplicons. Product availability reflects MBP's catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP </a>today to find high-fidelity DNA polymerase solutions for streamlining your laboratory workflows.

TruFi II DNA Polymerase - Product Sheet

Azura TruFi™ II DNA Polymerase

AZ-1702 by Azura Genomics is a 2 U/µL high-fidelity DNA polymerase with 3′→5′ proofreading activity, an error rate of 4.55 × 10⁻⁷ (~50× lower than Taq), and amplicon capability up to 10 kb. Supplied with a complete 5× buffer containing GC enhancers, dNTPs, and MgCl₂. Available in 200 U and 1,000 U formats.

Azura TruFi DNA Polymerase 200 units

Azura TruFi DNA Polymerase 1,000 units

Imaging and Gel Visualization

<h2>What Are Gel Documentation Systems?</h2>
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Gel documentation systems (gel docs, gel imagers, or gel visualization stations) are enclosed, darkroom-format imaging instruments that illuminate a gel or membrane from below or above using UV or visible-light sources, then capture a digital image with a mounted CCD or CMOS camera and analyze band intensities using integrated software. They replace manual photography (Polaroid film) with reproducible, quantitative digital images suitable for lab notebooks, publication figures, and archival. Key instrument components are: an enclosure (dark box or hood) to block ambient light; an illumination source (UV transilluminator at 302-365 nm, blue-LED at 470 nm, or white-light epi-illumination for Coomassie/silver-stained gels); a camera mounted above the gel (5-20+ megapixels, 8-bit or 16-bit); and imaging software for focus, exposure, and analysis. Choose a gel doc system based on the stains you use, whether quantitative analysis is required, and whether chemiluminescent western blot imaging (requiring a dark CCD or CMOS sensor) is part of your workflow.
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<h2 dir="ltr">What You Will Find:</h2>
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<ul>
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AzuraBlot Western Blotting Film &ndash; High-sensitivity, double-coated medical film designed for clear, high-contrast detection of chemiluminescent signals.
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Timed Electrophoresis Systems &ndash; Advanced 100-120V units with integrated time control to prevent overrunning and ensure consistent gel migration.
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Multicaster Imaging Kits &ndash; Specialized systems featuring versatile casting options to handle multiple gels simultaneously with professional-grade accuracy.
</li>
</ul>
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<h2>How to Choose a Gel Documentation System</h2>
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Camera resolution and bit depth
For routine agarose gel band recording (PCR verification, cloning confirmation), a 5-8 megapixel camera with 8-bit pixel depth (256 intensity levels) provides sufficient resolution. For quantitative densitometry, band comparison across samples, or publication-quality western blot figures, choose 12-20 megapixels with 16-bit depth (65,536 intensity levels). A 16-bit camera detects bands that differ by less than 10% in intensity accurately; 8-bit images compress the dynamic range and introduce quantification error for faint bands.
Illumination source compatibility
Gel documentation systems are available with UV (302 nm), blue-LED (470 nm), UV + white light (dual illumination), or multi-mode (UV + blue LED + white light + epi fluorescence for multiple dye formats). Systems with 302 nm UV transilluminators are compatible with ethidium bromide, SYBR Safe, and SafeView stains. Blue-LED systems at 470 nm are optimal for SYBR Safe, GelGreen, and SafeView Safe-Green, causing minimal DNA damage. Multi-mode systems provide the most workflow flexibility but carry a higher purchase cost. For SafeView Safe-Red (excitation 540 nm), a green-light source or white-light epi-illumination is needed.
Chemiluminescent western blot imaging
Chemiluminescent western blot detection requires a highly sensitive cooled CCD sensor (typically -25 to -40 degrees C cooling) to detect the low-level photon emission from HRP-substrate reactions. Chemiluminescence is not visible to the naked eye and requires long exposure (1 second to 30+ minutes); a gel doc system not rated for chemiluminescence will not capture western blot signals. Multi-purpose systems are designed for both gel fluorescence and chemiluminescent blot imaging on the same platform.
Software and analysis features
Integrated image analysis software should support: auto-exposure and motorized focus for hands-free gel capture; band detection and MW interpolation against a DNA ladder; lane profile plots for densitometry; TIFF and JPEG export for ImageJ and GraphPad compatibility; and electronic lab notebook (ELN) integration. Confirm that software licenses and updates are included with the instrument purchase and that the software is Windows 10/11 compatible.
Imaging area and gel format compatibility
The field of view (FOV) of the gel doc camera determines the maximum gel size imaged in a single capture. Standard FOV of 20 x 20 cm accommodates standard agarose gels and standard SDS-PAGE mini gels simultaneously. If you run 20 x 20 cm or larger wide-format agarose gels, confirm that the gel doc camera has a wide-angle lens or zoom that covers the full gel area without stitching multiple images.
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<h2>Specifications Context</h2>
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Gel documentation camera quantum efficiency (QE) above 70% in the visible and near-UV range is standard for research-grade systems; higher QE means more photons captured per exposure, enabling shorter exposure times and reduced photobleaching of gel bands. Motorized autofocus lens (8-48 mm focal range, F1.2 aperture) provides sharp focus across the full imaging area from mini to large-format gels without manual lens adjustment. 16-bit imaging dynamic range of 0-65,535 intensity values enables accurate densitometry of bands spanning a 100-fold concentration range on the same gel, while an 8-bit range (0-255) compresses signals from faint and intense bands into the same small numeric range. Integrated touchscreen gel doc systems with 12.5-inch displays eliminate the need for a connected PC for image capture, reducing benchtop complexity; USB and Wi-Fi export allow data transfer to laboratory information management systems (LIMS) and cloud storage without a tethered computer.
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Don&rsquo;t let your data fade away. Browse our imaging and visualization inventory and<a href="https://mbpinc.net/contact-us"> hit up the MBP team</a> for a custom quote today.

AzuraBlot Western Blotting Film

A8857 by Azura Genomics — AzuraBlot Western Blotting Film; double-sided emulsion; low background, high contrast; chemiluminescence and autoradiographic (¹⁴C, ³²P, ¹²⁵I, ³⁵S); 100 sheets/box; 5×7″ or 8×10″; 2-year shelf life.

AzuraBlot Western Blotting Film 5” x 7” 100 Sheets

AzuraBlot Western Blotting Film 8” x 10” 100 Sheets

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Product Literature

AzuraFlex™ cDNA Synthesis Kit

AZ-1997 AzuraFlex cDNA Synthesis Kit by Azura Genomics converts 10 pg–2 µg total RNA into cDNA (up to 9 kb) using MMLV-derived reverse transcriptase with random hexamer and oligo(dT) primers. 20 µl reactions; −20°C storage.

AzuraFlex cDNA Synthesis Kit 200 Reactions

AzuraFlex cDNA Synthesis Kit 1000 Reactions

Pipette Controller

<h2 dir="ltr"> What Are Pipette Controllers?</h2>
 A pipette controller (also called a pipetting aid, motorised pipette filler, or pipette boy) is a battery-operated instrument that accepts and drives 1&ndash;100 mL glass or plastic serological pipettes, replacing the rubber bulb, Pi-pump, and manual mouth-pipetting techniques. A motorised rotating wheel or roller generates controlled airflow to aspirate and dispense liquid at variable speeds; a hydrophobic 0.2 &micro;m inline filter prevents liquid and aerosol from reaching the motor. Controllers accept all standard serological pipette diameters (1 mL to 100 mL) through a silicone or rubber cone adapter. They are the standard dispensing tool for media preparation, cell culture reagent transfer, serial dilutions in large volumes, and reagent bottle-to-flask transfers. The disposable consumables used in the instrument. A pipette controller is required for safe, ergonomic use of serological pipettes in any volume from 1 mL to 100 mL &mdash; the rubber bulb is not a recommended alternative.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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Electronic pipette controllers for precise liquid control
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Manual pipette controllers for routine laboratory use
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Lightweight ergonomic pipette controllers
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Adjustable speed pipette controllers for controlled dispensing
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Controllers compatible with glass and plastic serological pipettes
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Sterile-use pipette controllers for sensitive applications
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High-precision aspirating controllers for accurate volume handling
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</ul>
<h2 dir="ltr"> How to Choose a Pipette Controller</h2>
 Power Source: Battery vs Rechargeable vs Mains Battery-powered controllers (AA or rechargeable internal battery) provide cordless flexibility across the bench and BSC. Rechargeable lithium-ion controllers offer 8&ndash;10 hours of continuous use per charge and charge from a base stand. Mains-powered controllers provide continuous operation without charge interruption &mdash; preferred in high-throughput cell culture facilities. Speed Control Two-speed controllers offer slow (for precise fill) and fast (for rapid dispense) modes. Variable-speed controllers with a continuous-pressure or stepless trigger allow fine-tuned flow rate adjustment for viscous media, delicate cell suspensions, or filling against gravity into narrow-neck vessels. Filter: Inline 0.2 &micro;m Hydrophobic Filter A 0.2 &micro;m hydrophobic inline filter is the critical protection component &mdash; it prevents liquid from entering the motor during accidental over-aspiration. Filters should be replaced when liquid reaches the filter or when flow resistance increases noticeably; replacement filters are sold separately for most models. Universal Cone Compatibility Confirm the controller's cone adapter accepts your pipette brand's tip diameter. Most controllers use a silicone or rubber cone that accommodates 1&ndash;100 mL serological pipettes from Falcon, Globe Scientific, and Nest Scientific without adapters. Autoclavability Controllers intended for BSC and sterile cell culture use should have an autoclavable cone, nose piece, and filter assembly. They have autoclavable lower sections; the battery/electronic module is not autoclavable. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard pipette controllers accept serological pipette diameters from 1 mL (narrow shaft) through 50 mL and 100 mL (wide-body). Replacement 0.2 &micro;m hydrophobic filters are sold in packs; filter replacement interval is typically every 6&ndash;12 months or immediately after liquid ingress. It has a runtime of approximately 8 hours per charge on a single lithium-ion battery.&nbsp; 
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Control your workflow with ease&mdash;designed for smooth aspiration, consistent dispensing, and dependable performance at every step. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP Team now.</a>

AzuraPette REACH, Long-Neck Pipette Controller, w/ QuickStand™, 115V US

A6950 by Azura Genomics is the AzuraPette REACH™ long-neck motorized serological pipette controller with QuickStand™ retractable kickstand, pressure-sensitive speed control, 8-hour rechargeable battery, and 0.8 µm inline filter. 115V US. Designed for ergonomic pipetting in biosafety cabinets.

Single Channel Pipettors

<h2 dir="ltr">What Are Single-Channel Pipettors?</h2>
 A single-channel pipettor is the single most widely used liquid handling instrument in molecular biology, cell biology, and clinical research. It transfers precise volumes between 0.1 &micro;L and 5,000 &micro;L, one well or tube at a time, using an air-displacement piston mechanism. The piston creates a partial vacuum that aspirates liquid into a disposable tip; advancing the piston fully dispenses the liquid. A spring-loaded tip ejector releases the used tip without hand contact for contamination control. Manual models depend on finger force and offer simple, battery-free operation; electronic models add programmable aspiration/dispensing speeds, multi-dispense modes, and up to 5 custom pipetting programs.&nbsp; A set of four manual single-channel pipettors (P10, P20, P200, P1000) covers 95% of molecular biology liquid handling; add electronic models for high-throughput repetitive work.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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Adjustable volume single-channel pipettors for flexible workflows
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Fixed volume pipettors for repeatable dispensing tasks
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Mechanical pipettors for standard lab use
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Ultra-low volume micropipettors for precise &micro;L handling
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Wide-range pipettors covering &micro;L to mL volumes
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Autoclavable pipettors for sterile environments
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Lightweight ergonomic pipettors for long sessions
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</ul>
<h2 dir="ltr"> How to Choose a Single-Channel Pipettor</h2>
 Volume Range: Build a Complete Bench Set A standard four-pipettor bench set covers P10 (1&ndash;10 &micro;L), P20 (2&ndash;20 &micro;L), P200 (20&ndash;200 &micro;L), and P1000 (100&ndash;1,000 &micro;L). For sub-microliter work (sequencing, single-cell), add a P2 (0.2&ndash;2 &micro;L). For large-volume dispensing (media prep, column loading), add a P5000 (1,000&ndash;5,000 &micro;L). Avoid selecting a volume range that places your target dispense below 20% of the maximum &mdash; accuracy degrades at the low end of any range. Manual vs Electronic Manual single-channel pipettors (Eppendorf Research Plus, Gilson PIPETMAN Classic, Rainin Pipet-Lite XLS+) are the standard for routine lab work: field-calibratable, autoclavable, and requiring no power source. Electronic single-channel pipettors (Eppendorf Research Pro, Rainin Pipet-Lite XLS+ Electronic) add: programmable aspiration/dispense speed (5 speeds), multi-dispense (repeat pipetting) mode, mix mode, dilution mode, and backlight LCD volume display. Electronic models reduce hand fatigue in workflows exceeding 200&ndash;300 tip changes per day. Tip Compatibility Three tip systems cover the market: standard conical (universal fit &mdash; fits Eppendorf Research Plus, Gilson PIPETMAN, Rainin non-LTS, Thermo, Sartorius/BioHit); Rainin LTS cylindrical (requires LTS-specific tips &mdash; lower force insertion); Eppendorf epT.I.P.S. proprietary (tightest fit for Eppendorf instruments). Select the tip system that matches your pipettor purchase and existing tip inventory before committing to a brand. Autoclavability and Chemical Resistance Manual pipettors from Eppendorf, Gilson, and Rainin have fully autoclavable lower sections (121&deg;C, 15 psi, 15 min). Electronic pipettors have autoclavable nose cones only &mdash; the electronic module cannot be autoclaved. Calibration and Service All single-channel pipettors are factory-calibrated per ISO 8655. Field recalibration tools are available for Eppendorf, Gilson, and Rainin to perform in-lab gravimetric verification without external service. 
<h2 dir="ltr"> Specifications Context </h2>
 ISO 8655 Part 2 accuracy spec for a 200 &micro;L air-displacement pipettor: &plusmn;0.8 &micro;L systematic error, &le;0.3 &micro;L random error (Eppendorf Research Plus specification at 200 &micro;L). Most manual pipettors weigh 70&ndash;120 g; electronic models weigh 150&ndash;220 g. Operating temperature range: typically 15&ndash;40&deg;C. Eppendorf Research Plus, Gilson PIPETMAN Classic, and Rainin Pipet-Lite XLS+ LTS remain the three dominant single-channel pipettor platforms in North American research labs.&nbsp; 
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Ready to find your new favorite lab tool? Don&rsquo;t just settle for any grip&mdash;find the one that fits your hand and your science. <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Reach out to the MBP team</a> today for a customized quote.

AzuraPette Single Channel Pipette

A5200-100U by Azura Genomics is the AzuraPette™ single-channel adjustable pipette for the 10–100 µL range. Features Roto-Lock™ volume adjustment, reduced actuation forces, and a lightweight ergonomic body designed for prolonged pipetting sessions.

AzuraPette Single Channel Pipette, 10 to 100ul

AzuraPette Single Channel Pipette, 1-10 mL

AzuraPette Single Channel Pipette, 1.0 to 10ul

AzuraPette™ Single Channel Pipette, 100 to 1000ul

AzuraPette™ Single Channel Pipette, 20 to 200ul

AzuraPette™ Single Channel Pipette, 2.0 to 20ul

AzuraPette™ Single Channel Pipette, 0.2 to 2ul

AzuraPette™ Single Channel Pipette, 30 to 300ul

AzuraPette™ Single Channel Pipette, 5.0 to 50ul

AzuraPette™ Single Channel Pipette, 1-5 mL

SureStand™ Pipette Stand for 6 pipettes, up to four multi-channels, acrylic

AzuraPette™ Starter Kit, includes 1 Pipette Carousel and 4 Pipettes, 10ul, 20ul, 200ul, 1000ul

Multi-Channel Pipettors

<h2 dir="ltr">What Are Multi-Channel Pipettors?</h2>
 A multi-channel pipettor contains 8 or 12 piston-tip cone assemblies arranged in a single row at 9 mm centre-to-centre spacing &mdash; matching the well spacing of standard 96-well plates &mdash; allowing an operator to simultaneously dispense into an entire plate row (12-channel) or column (8-channel) with a single plunger depression. This reduces the time to fill a 96-well plate from ~96 individual dispenses to 12 (8-channel) or 8 (12-channel). For 384-well plates, 16-channel models or adjustable-spacing multichannel pipettors accommodate the 4.5 mm inter-well spacing. Multi-channel pipettors are the standard instrument for ELISA plate loading, serial dilution across 96-well plates, PCR master-mix dispensing, and cell-based assay setup. An 8-channel pipettor fills a 96-well plate in 12 strokes; a 12-channel pipettor fills it in 8 &mdash; choose based on whether your workflow is row-based or column-based.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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8-channel adjustable volume multi-channel pipettors
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12-channel high-throughput pipettors
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Mechanical multi-channel pipettors for routine use
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Autoclavable multi-channel pipettors
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Ergonomic lightweight pipettors for extended use
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Calibrated precision multi-channel pipettors
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Microplate-compatible pipettors for 96-well formats
</li>
</ul>
<h2 dir="ltr"> How to Choose a Multi-Channel Pipettor</h2>
 8-Channel vs 12-Channel 8-channel pipettors (8 rows) are used when working column-by-column across a 96-well plate &mdash; the standard for PCR setup, ELISA, and serial dilutions where samples run down columns. 12-channel pipettors (12 columns) are used when filling row-by-row, common for reagent dispensing from reservoirs across all 12 columns simultaneously. Both configurations dispense the same 96 wells per plate but in different orientations. Volume Range Common multi-channel volume ranges: 0.5&ndash;10 &micro;L (for low-volume PCR or sequencing), 5&ndash;50 &micro;L, 10&ndash;100 &micro;L, and 20&ndash;200 &micro;L (the most widely used range for ELISA and cell-based assays), and 30&ndash;300 &micro;L. Select the range that places your most frequent dispense volume at 50&ndash;80% of the maximum for best accuracy. Manual vs Electronic Manual multi-channel pipettors (Eppendorf Research Plus Multichannel, Gilson PIPETMAN Multichannel) require no battery and are field-calibratable. Electronic multi-channel pipettors (Eppendorf Xplorer Multichannel, Rainin Pipet-Lite L8 LTS Electronic) add programmable speed, multi-dispense mode, and reduced plunger force &mdash; particularly valuable for high-throughput plate workflows. Tip Compatibility Multi-channel pipettors use the same tip collar geometry as single-channel models: standard conical for Eppendorf Research Plus and Gilson PIPETMAN (universal-fit 8-strip tips); LTS cylindrical for Rainin L8/L12 LTS (LTS 8-strip tips). Confirm tip format matches the multichannel instrument before ordering tip strips. Adjustable Tip Spacing Eppendorf Research Plus Move It and equivalent adjustable-spacing models allow spacing between channels to be set to 4, 6, 8, or 12 mm &mdash; enabling use with 24-well, 48-well, and 384-well plate formats without a dedicated instrument for each format. 
<h2 dir="ltr"> Specifications Context</h2>
 Multi-channel pipettors are subject to ISO 8655 Part 6 (multichannel piston-operated volumetric instruments). Standard accuracy specification for a 200 &micro;L 8-channel model is &plusmn;1.5 &micro;L systematic error; inter-channel CV should be &le;1.0% to ensure well-to-well reproducibility in plate-based assays. Autoclavable lower sections are standard on Eppendorf Research Plus and Gilson PIPETMAN multichannel models. Eppendorf Research Plus Multichannel and Rainin Pipet-Lite L8/L12 LTS remain the dominant manual multichannel platforms in North American research labs.&nbsp; 
&nbsp;
Tired of the one-well-at-a-time grind? Let&rsquo;s speed things up.<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">&nbsp;Reach out to the MBP team</a> today for a quote on the multi-channel system that fits your lab&rsquo;s pace.

AzuraPette™ 12-Channel Pipette

AzuraPette™ 12-Channel Pipette (A5212-10U) by Azura Genomics — 1.0–10 µL volume range, 12-channel, lightweight low-force design with Roto-Lock™ volume adjustment. Ideal for 96-well plate liquid-handling workflows.

AzuraPette™ 12-Channel Pipette, 1.0 to 10ul

AzuraPette™ 12-Channel Pipette, 20 to 200ul

AzuraPette™ 12-Channel Pipette, 2.0 to 20ul

AzuraPette™ 12-Channel Pipette, 30 to 300ul

AzuraPette™ Pipette Controller with QuickStand

AzuraPette™ Pipette Controller with QuickStand (A6080) by Azura Genomics — 6 oz, ambidextrous, 8-hour rechargeable battery (3.7 V/750 mAh), pressure-sensitive speed control, 0.45 µm aerosol filter, patented retractable QuickStand™ kickstand. For 10 mL, 25 mL, and 50 mL serological pipettes.

AzuraPette™ Pipette Controller, w/ QuickStand™

Replacement battery, lithium, 3.7V, 750mAh

Replacement filter, 0.45um, pack of 5

Replacement Charger for Electronic Pipette Controller

SYBR/Primer-Probe

<h2 dir="ltr"> What Is a SYBR/Primer-Probe Master Mix?</h2>
 A SYBR/Primer-Probe master mix is a 2x formulation positioned to support both dye-based (SYBR Green) and primer-probe (hydrolysis probe, TaqMan-style) detection chemistries, typically built on a common hot-start Taq, dNTP, MgCl2, and buffer backbone with formulation adjustments that accommodate either an intercalating dye or a labelled probe, depending on the assay. Choose this combined format when your lab runs both SYBR Green and probe-based assays and wants to standardise on fewer master mix products across the qPCR workflow.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="tosb50" data-start="110" data-end="247">One-step RT-qPCR kits that combine reverse transcription and real-time PCR in a single tube to streamline RNA quantification workflows.</li>
<li data-section-id="11s385w" data-start="248" data-end="363">Hot-start enzyme systems are designed to reduce non-specific amplification during reaction setup and thermal cycling.</li>
<li data-section-id="1sl7e5s" data-start="364" data-end="494">Probe-based detection chemistries for specific RNA target quantification in gene expression and pathogen detection applications.</li>
<li data-section-id="7bdacc" data-start="495" data-end="613">HiROX, LoROX, and NoROX formulation options to support compatibility with a wide range of real-time PCR instruments.</li>
<li data-section-id="19ytf2e" data-start="614" data-end="737">Optimized one-step workflows that reduce sample handling and help minimize contamination risk in high-throughput studies.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR/Primer-Probe Master Mix</h2>
 Assay Portfolio Mix Labs running a mix of SYBR Green gene expression assays and probe-based genotyping or pathogen-detection assays can benefit from standardising on a combined-compatible master mix line, reducing the number of distinct products to validate, store, and track across the facility. Reference Dye Configuration Confirm whether the specific product includes ROX or other passive reference dyes as standard, since probe-based assays in particular often rely on ROX for instrument normalisation, while some SYBR-only workflows may use ROX-free instruments. Validation Across Chemistries When adopting a combined-compatible master mix for the first time, validating performance separately for both a representative SYBR Green assay and a representative probe-based assay is recommended, since buffer optimisations that favour one chemistry slightly can sometimes trade off against the other. Primer and Probe Concentration Guidelines Typical primer-probe qPCR setups use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M; confirm the master mix manufacturer's recommended concentration ranges for both components when switching from a single-chemistry product. Throughput and Inventory Simplification For core facilities supporting multiple research groups with different assay types, a combined-compatible master mix can simplify inventory management and reduce the risk of using the wrong master mix for a given assay type. 
<h2 dir="ltr"> Specifications Context</h2>
 Standard real-time PCR master mixes are 2x concentrated and contain dNTPs, MgCl2, and DNA polymerase as their common backbone; a SYBR/primer-probe combined format extends this backbone with formulation choices intended to perform acceptably whether the researcher adds SYBR Green dye for dye-based detection or a labelled probe for probe-based detection. In probe-based setups, primer pairs are commonly used at a final concentration around 0.5 &micro;M with probes at 0.25-0.5 &micro;M, while SYBR-based protocols may additionally include a small amount of random hexamer primer in RT-qPCR contexts. Real-time PCR master mixes of this type are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-yield&nbsp;SYBR/Primer-Probe Master Mixes for your lab.

AzuraQuant Probe 1-Step qPCR Mix - Product Sheet

AzuraQuant Probe 1-Step qPCR Mix HiRox

AZ-3901 AzuraQuant Probe 1-Step qPCR Mix HiRox by Azura Genomics is a 2× RT-qPCR master mix with high ROX reference dye, compatible with TaqMan® and Scorpions® probes, 1 pg–1 µg RNA input, 20 µl reactions, stored at −20°C.

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 500 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit HiRox 5,000 reactions

AzuraQuant Probe 1-Step qPCR Mix LoRox

AZ-4101 by Azura Genomics is the AzuraQuant Probe 1-Step qPCR Mix LoRox — a 2× hot-start RT-qPCR master mix with 20× AzuraSprint Reverse Transcriptase for one-tube RT-qPCR using TaqMan, Scorpions, or molecular beacon probes. Available from 100 to 10,000 reactions. LoRox formulation for low passive reference instruments.

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 100 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit LoRox 500 reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 1,000 Reactions

AzuraQuant Probe One-Step qPCR Mix LoRox 5,000 Reactions

AzuraQuant Probe 1-Step qPCR Mix NoRox

AZ-4301 by Azura Genomics is AzuraQuant™ Probe 1-Step qPCR Mix NoRox — a 2× RT-qPCR master mix containing Azura HS Taq and AzuraSprint thermostable RT. Compatible with TaqMan®, Scorpions®, and molecular beacon probes. Available in 100 to 10,000 reaction formats. No ROX passive reference dye.

AzuraQuant Probe 1-Step qPCR Mix No-Rox 100 Reactions

AzuraQuant Probe 1-Step qPCR Mix No-Rox 500 Reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 1,000 reactions

AzuraQuant Probe 1-Step qRT-PCR Kit NoRox 5,000 reactions

Primer-Probe

<h2 dir="ltr"> What Is a Primer-Probe Master Mix?</h2>
 A primer-probe master mix is a 2x concentrated formulation built around a hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimised buffer, designed for use with hydrolysis-probe detection chemistries such as TaqMan. Unlike SYBR Green dye-based mixes, primer-probe formulations rely on a separately added, fluorescently labelled, sequence-specific probe combined with target-specific primers to generate the detection signal, with specificity coming from both primer and probe sequence matching. Choose a primer-probe master mix when assay specificity is critical, when multiplexing several targets in one well is needed, or when working with an established probe-based assay design.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="qhwnhl" data-start="279" data-end="413">Probe-based qPCR master mixes with HiROX, LoROX, and ROX-free options for compatibility across a range of real-time PCR instruments.</li>
<li data-section-id="1cnihmh" data-start="414" data-end="543">Hot-start polymerase formulations designed to support specific target detection in hydrolysis probe (TaqMan-style) qPCR assays.</li>
<li data-section-id="51tf3n" data-start="544" data-end="643">Blue tracking dye probe master mixes for easier reaction setup and visual pipetting confirmation.</li>
<li data-section-id="cjeacg" data-start="644" data-end="749">2X ready-to-use probe qPCR master mixes containing optimized buffers, dNTPs, and polymerase components.</li>
<li data-section-id="xfwfro" data-start="750" data-end="884">One-step probe RT-qPCR formats that combine reverse transcription and real-time PCR amplification in a single tube for RNA analysis.</li>
</ul>
<h2 dir="ltr"> How to Choose a Primer-Probe Master Mix</h2>
 Reference Dye Inclusion Primer-probe master mixes commonly include ROX as a passive reference dye for signal normalisation across wells on instruments that require it -- confirm whether your real-time PCR instrument needs ROX and whether the chosen master mix includes it or requires separate addition. Multiplexing Capability A core advantage of probe-based chemistry is multiplexing -- detecting multiple targets in a single well using probes labelled with spectrally distinct fluorophores. Confirm the master mix is validated for the number of multiplexed targets your assay requires. Probe Chemistry Compatibility While TaqMan-style hydrolysis probes are the most common, other probe chemistries such as Scorpion primers, exist; confirm the master mix is formulated for compatibility with your specific probe chemistry. Primer and Probe Concentration Guidelines Standard setups commonly use primer pairs at a final concentration around 0.5 &micro;M and probes at 0.25-0.5 &micro;M -- confirm the master mix manufacturer's recommended ranges, since probe concentration affects both signal intensity and background. One-Step vs. Two-Step Application Primer-probe master mixes are used in both two-step workflows (with a separately generated cDNA template) and as the chemistry component within one-step RT-qPCR kits -- confirm which configuration matches your workflow when selecting a product. 
<h2 dir="ltr"> Specifications Context</h2>
 In probe-based qPCR, the 5'-3' exonuclease activity of the Taq DNA polymerase cleaves the dual-labelled probe annealed to the target sequence during amplification, separating a fluorescent reporter from its quencher and generating signal proportional to target abundance -- the mechanism underlying the widely used TaqMan probe system. Because both primer and probe sequence must match the target for signal generation, probe-based assays generally offer higher specificity than dye-based detection, and because probes can be labelled with spectrally distinct fluorophores, multiple targets can be detected in a single well. Standard reaction setup adds primer pairs at approximately 0.5 &micro;M and probes at 0.25-0.5 &micro;M to the 2x master mix. Primer-probe master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized Primer-Probe real-time master mixes for your lab.

AzuraQuant Probe Fast qPCR Mix HiRox

AZ-2701 by Azura Genomics — AzuraQuant Probe Fast qPCR Mix HiRox; 2× probe-based mix; high ROX passive reference; TaqMan®, Scorpions®, molecular beacon compatible; antibody hot-start; standard and fast cycling; 100–5000 rxn formats.